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The Purification Of Mcrb And Mcrc Proteins In Mcrbc Restriction System Of Escherichia Coli K12 And Dna Sequencing Of The Region Downstream Of Mcrc., Ling Zheng Jan 1992

The Purification Of Mcrb And Mcrc Proteins In Mcrbc Restriction System Of Escherichia Coli K12 And Dna Sequencing Of The Region Downstream Of Mcrc., Ling Zheng

LSU Historical Dissertations and Theses

The McrBC restriction system of Escherichia coli K12 degrades methylcytosine-containing foreign DNA. Two genes mcrB and mcrC had been shown to be required for the McrBC restriction (Ross, et al., J. Bacteriol., 1989b). To explore the involvement of additional gene(s), a 1.7 kb fragment downstream of the mcrC was cloned and sequenced. Also mutants containing deletion in mcrBC or the downstream region were isolated by transposon excision. Genetic complementation studies confirmed that only DNA containing mcrB and mcrC gene on a high copy plasmid were needed to fully restored McrBC restriction. Deletion of the 1.7 kb fragment from or addition …

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The Keepers And The Kept: The First Hundred Years Of The Tennessee State Prison System, 1830-1930. (Volumes I And Ii)., Larry D. Gossett Jan 1992

The Keepers And The Kept: The First Hundred Years Of The Tennessee State Prison System, 1830-1930. (Volumes I And Ii)., Larry D. Gossett

LSU Historical Dissertations and Theses

Tennessee built its first state penitentiary in Nashville in 1830 on Sixteenth and Church Streets on land that is now a downtown parking lot. Revisions in the criminal code made many offenses punishable by imprisonment instead of corporal or capital punishment, and the convict population grew steadily. Continuing concern was expressed by the General Assembly that the penitentiary be self-supporting, and much effort was expended at all levels of government to accomplish that end. This study covers the history of approximately the first one hundred years of the Tennessee State Prison System. It begins with an overview of the criminal …

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American Civil Religion And The Presidential Rhetoric Of Jimmy Carter., Michael James Adee Jan 1992

American Civil Religion And The Presidential Rhetoric Of Jimmy Carter., Michael James Adee

LSU Historical Dissertations and Theses

Scholars have long speculated about the role of American civil religion and political discourse. Of particular interest to rhetorical scholars is the function of a civil religion idiom within presidential discourse. The cornerstone of this study is founded upon Rod Hart's ideas in The Political Pulpit (1977) wherein the nature of American civil religion is described through the metaphor of a legal contract. A rhetorical approach to civil religion provides the framework for this study. Nine major public speeches by Carter as candidate and president, from 1974 to 1979, are examined in order to locate and identify the symbolic breeches …

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Comparative Study Of Anionic And Nonionic/Anionic Surfactant Systems In Micellar Electrokinetic Capillary Chromatography., Edward Lester Little Jan 1992

Comparative Study Of Anionic And Nonionic/Anionic Surfactant Systems In Micellar Electrokinetic Capillary Chromatography., Edward Lester Little

LSU Historical Dissertations and Theses

Micellar electrokinetic capillary chromatography (MECC) is a rapidly growing technique allowing separation of electrically neutral compounds with the high separation efficiencies typically observed in capillary zone electrophoresis. This technique is still in its infancy and subsequently suffers from several inherent problems which must be addressed such as the limited range of solute elution and clear protocol for optimization of separations. This work addresses these problems with primary emphasis on using mixed nonionic/anionic surfactant systems as opposed to pure surfactants. In Chapter One, the fundamental theory behind MECC is discussed along with a brief synopsis of previous studies involving different surfactant …

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Louisiana Agriculture Magazine, Fall 1991, Lsu Agricultural Experiment Station Oct 1991

Louisiana Agriculture Magazine, Fall 1991, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

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Theoretical Aspects Of Intense Field Harmonic Generation, Anne L. Huillier, Kenneth J. Schafer, Kenneth C. Kulander Aug 1991

Theoretical Aspects Of Intense Field Harmonic Generation, Anne L. Huillier, Kenneth J. Schafer, Kenneth C. Kulander

Faculty Publications

We present theoretical studies of high-order harmonic generation in a rare-gas medium. The experimental results obtained at Saclay with a 1064 nm Nd-YAG laser in the 1013 W cm-2 intensity range are summarized. The harmonic emission strengths first decrease rather steeply for the first orders, then form a long plateau up to the 21st harmonic in xenon, or up to the 33rd harmonic in argon, before decreasing again rather abruptly. The theoretical description of these experiments consists first in the calculation of the photoemission spectra emitted by a single atom. The spectra are obtained by numerically integrating a time-dependent Schrôdinger …

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Louisiana Agriculture Magazine, Summer 1991, Lsu Agricultural Experiment Station Jul 1991

Louisiana Agriculture Magazine, Summer 1991, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

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Louisiana Agriculture Magazine, Spring 1991, Lsu Agricultural Experiment Station Apr 1991

Louisiana Agriculture Magazine, Spring 1991, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

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The Effect Of Co-Culture Systems From Non-Reproductive Origins On The Development Of Superovulated And In Vitro Fertilized Bovine Embryos., Hak-Nam Kim Jan 1991

The Effect Of Co-Culture Systems From Non-Reproductive Origins On The Development Of Superovulated And In Vitro Fertilized Bovine Embryos., Hak-Nam Kim

LSU Historical Dissertations and Theses

The objective of this experiment was to compare in vitro development of bovine embryos obtained from superovulation and in vitro fertilization (IVF) in two unique monolayer co-culture systems, bovine fetal spleen cell monolayers (BFS) and chick embryo fibroblast monolayers (CEF). To test the possible harmful effects of these co-culture systems, 225 murine morula-stage embryos were cultured for 72 hours with these monolayers. The percentage of hatched embryos was 62.7% in culture medium alone, 76% in BFS and 57.3% in CEF. BFS and CEF did not induce any detectable harmful effect on these developing murine embryos. When 60 transferable morula-stage bovine …

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Simplified Procedure For The Isolation Of Intact Chloroplasts From Chlamydomonas Reinhardtii, Catherine B. Mason, Sharon Matthews, Terry M. Bricker, James V. Moroney Jan 1991

Simplified Procedure For The Isolation Of Intact Chloroplasts From Chlamydomonas Reinhardtii, Catherine B. Mason, Sharon Matthews, Terry M. Bricker, James V. Moroney

Faculty Publications

A simple procedure that yields highly purified intact chloroplasts from Chlamydomonas reinhardtii is described. This procedure involves breakage of cell wall-deficient cells by passing them through a narrow bore syringe needle. The intact chloroplasts are then purified from the crude homogenate by differential centrifugation and Percoll gradient centrifugation. This procedure generates relatively high yields of chloroplasts capable of CO2 fixation. These chloroplasts were characterized by electron microscopy, marker enzyme analysis, and ferricyanide exclusion. Transmission electron microscopy indicates that these chloroplasts retain their pyrenoids and eyespots. Scanning electron microscopy confirms that the characteristic cup shape of C. reinhardtii chloroplasts persists in …

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A Quest For Legitimacy: Impression Management Strategies Used By An Organization In Crisis., Rachel Harris Caillouet Jan 1991

A Quest For Legitimacy: Impression Management Strategies Used By An Organization In Crisis., Rachel Harris Caillouet

LSU Historical Dissertations and Theses

The purpose of this study was to investigate impression management strategies used by an organization in crisis and to develop a typology of impression management strategies used by an organization. The focus of the study was Marine Shale Processors (MSP), an environmental company located in Amelia, Louisiana, which recycles hazardous and non-hazardous materials into a non-toxic aggregate. Impression management strategies used by MSP in statements to stakeholders were examined using multiple methods. Also, the study examined whether or not different strategies were utilized with different stakeholders, whether or not management and non-management relied on the same strategies with stakeholders, and …

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Genetics Of Resistance To Preharvest Aflatoxin Accumulation In Maize Containing The Lfy Gene., Daniel Preston Gorman Jan 1991

Genetics Of Resistance To Preharvest Aflatoxin Accumulation In Maize Containing The Lfy Gene., Daniel Preston Gorman

LSU Historical Dissertations and Theses

Preharvest infection of maize (Zea mays L.) grain by Aspergillus flavus Link ex Fries and subsequent aflatoxin contamination is a serious problem, especially in the southeastern USA. Studies on the genetics of resistance to aflatoxin contamination in maize are quite limited, but a better understanding of this aspect is needed for developing resistant germplasm. The objective of the first field study was to determine the genetics of resistance to aflatoxin contamination in maize possessing the leafy (Lfy) gene. Seven Lfy synthetic genotypes were crossed in a diallel fashion, and the resulting 21 single crosses were evaluated for aflatoxin contamination in …

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Stereoelectronics Of Carboxylate-Imidazolium Hydrogen Bonds In Models Of The Aspartate-Histidine Couple In Serine Proteases., Nabeel Abdel Raouf Nabulsi Jan 1991

Stereoelectronics Of Carboxylate-Imidazolium Hydrogen Bonds In Models Of The Aspartate-Histidine Couple In Serine Proteases., Nabeel Abdel Raouf Nabulsi

LSU Historical Dissertations and Theses

The geometry of the carboxylate-imidazolium hydrogen bond in the crystalline state and the effect of microenvironment are investigated in models of the aspartate-histidine (Asp-His) couple. Synthetic methods leading to the preparation of intramolecular models, possessing syn and anti-oriented hydrogen bonds, also are described. A single-pot procedure has been developed for converting phenoxyacetonitriles into their respective benzimidazole derivative in higher yields than the traditional two step procedure. An efficient single-pot procedure also has been developed for converting benzofuran to its acetylene derivative, 2-acetoxyphenylacetylene. This method involves in situ acetylation of the unstable phenol intermediate. Nine intermolecular imidazolium-benzoate couples have been prepared …

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A Study Of The Metal Clusters In The Nitrogenase Proteins Of Azotobacter Vinelandii., Melinda Ehrlich Oliver Jan 1991

A Study Of The Metal Clusters In The Nitrogenase Proteins Of Azotobacter Vinelandii., Melinda Ehrlich Oliver

LSU Historical Dissertations and Theses

Electron Paramagnetic Resonance (EPR) spectroscopy was used to examine the paramagnetism of the clusters in nitrogenase from Azotobacter vinelandii. The EPR spectrum of nitrogenase (Av1 and Av2) was examined in the presence of an extrinsic paramagnet, DyEDTA, at different concentrations of NaCl, during thionine oxidation (Av1), and in the presence of MgATP (Av2). Cross combinations of Av1 and Av2 were also investigated in the presence and absence of the chemical cross-linker, EDC as well as with the nitrogenase components from Clostridium pasteurianum. From relaxation enhancement measurements, the distance of closest approach of DyEDTA to the intrinsic metal clusters in Av1 …

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Isolation And Characterization Of Macrophage Inhibitory Compounds From Mycobacterium Paratuberculosis., Murray Elison Hines Ii Jan 1991

Isolation And Characterization Of Macrophage Inhibitory Compounds From Mycobacterium Paratuberculosis., Murray Elison Hines Ii

LSU Historical Dissertations and Theses

Fractions exhibiting activity detrimental to the killing function of activated bovine monocyte/macrophages towards Candida albicans were obtained from Mycobacterium paratuberculosis. Active fractions were initially derived by ultrasonication of bacterial samples followed by ammonium sulfate precipitation, ion-exchange chromatography, gel filtration chromatography, heat precipitation, acid precipitation and alkali treatment. Two different fractions that exhibited marked inhibition of macrophage killing ability were derived and partially characterized. Novel methodology was developed to accomplish the task of lysis and fractionation of mycobacteria utilizing the Matrix Solid Phase Dispersion (MSPD) technique that appeared to be much more efficient and is certainly less time consuming and less …

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A Study Of The Effects Of Prediction Activities On Instructional Outcomes In High School Genetics., Anne Spratlan Sinclair Jan 1991

A Study Of The Effects Of Prediction Activities On Instructional Outcomes In High School Genetics., Anne Spratlan Sinclair

LSU Historical Dissertations and Theses

The purpose of this study was to examine the instructional effects of incorporating prediction activities in a high school biology genetics curriculum. Criteria for instructional effectiveness included enhanced levels of classroom discussion and interaction, improved subject-related attitude, higher achievement motivation, and greater mastery of genetics concepts. Genetics was chosen as the domain of research because of the multiple variables operating which make it amenable to the making of predictions. Four experienced high school biology teachers taught an experimental and a control class. Students in the experimental classes made written predictions using researcher-developed prediction activities as an introduction to 19 genetics …

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The Use Of Supertitles By American Opera Companies., Christina Margaret Alves Jan 1991

The Use Of Supertitles By American Opera Companies., Christina Margaret Alves

LSU Historical Dissertations and Theses

The main purpose of this monograph was to examine the use of supertitles in foreign language opera by American opera companies. Supertitles are the translation of an opera's text projected simultaneously above the stage during an opera performance. The use of supertitles is one of the most controversial issues in the opera world. Although the primary purpose is to enable the audience to understand a foreign text, there is an ongoing controversy over whether supertitles enhance an opera performance or detract from it. Many opera companies are using supertitles, but there are still those who have not made the investment …

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Poly(Arylene Ether Sulfones): Synthesis, Modifications And Applications., Marietta Navarro Aniano-Ilao Jan 1991

Poly(Arylene Ether Sulfones): Synthesis, Modifications And Applications., Marietta Navarro Aniano-Ilao

LSU Historical Dissertations and Theses

Poly(arylene ether sulfones) were synthesized by aromatic nucleophilic polymerization of the monomers; 4,4$\sp\prime$-biphenol, 4-chlorophenyl sulfone, hydroquinone, and tert-butylhydroquinone. Different conditions of temperature and solvent systems were used to determine the best synthetic approaches for the preparation of poly-(4,4$\sp\prime$-biphenol/4-chlorophenyl sulfone) (1:1), poly-(4-4$\sp\prime$-biphenol/4-chlorophenyl sulfone/hydroquinone) (1:2:1), and poly (4-4$\sp\prime$-biphenol/4-chlorophenyl sulfone/tert-butylhydroquinone) (1:2:1). The use of aprotic high boiling solvent mixtures of N-methyl-2-pyrrolidone (NMP), and N-cyclohexyl-2-pyrrolidone (CHP), facilitated the formation of polymers with structures which can easily be controlled by varying the ratio of monomers, and by varying the concentration of reactants. The polymers were functionalized and modified by silylation, amination and aminomethylation. Directed silylation …

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Social Networks And Linguistic Accommodation Of Mainland Chinese In An Urban American Chinese Community., Hong Chi Jan 1991

Social Networks And Linguistic Accommodation Of Mainland Chinese In An Urban American Chinese Community., Hong Chi

LSU Historical Dissertations and Theses

The growing number of immigrants from both Taiwan and Mainland China have brought speakers of different Mandarin varieties into contact in an American context. In the Chinese community in Los Angeles, Mainland Putonghua speakers are found to accommodate their language to the local Taiwan Mandarin speakers. Social network analysis, a model oriented toward the individual, is adapted here for analyzing this linguistic situation. The conversation of 35 informants was recorded within a naturally occurring context and their social network scores were compared with their scores for nine linguistic variables selected for this study. It was found that the accommodation process …

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Nuclear Transplantation: Intracellular Release And Extracellular Influx Of Calcium In Response To Electrofusion Pulse And Its Effect On Murine Oocyte Activation And Embryonic Development., Lee F. Rickords Jan 1991

Nuclear Transplantation: Intracellular Release And Extracellular Influx Of Calcium In Response To Electrofusion Pulse And Its Effect On Murine Oocyte Activation And Embryonic Development., Lee F. Rickords

LSU Historical Dissertations and Theses

The transplantation of early embryonic cell nuclei into oocytes in which the maternal chromosomes have been removed is a method by which multiple offspring of the same genotype can be produced. One critical factor required for successful development of nuclear transfer derived embryos is inducing the release of the recipient ooplasm from meiotic arrest (activation). This is accomplished during fusion of the two cells. The DC electrofusion pulse used to fuse the two cells together also activates the recipient ooplasm to resume meiotic events, however, the efficiency of electrofusion pulse induced activation remains low. It is known that activation of …

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The Differential Effects Of Either X-Irradiation Or Thermal Stress On The Two Major Cell Types Of The Blastocyst Stage Murine Embryo., Joe Steve Capehart Jan 1991

The Differential Effects Of Either X-Irradiation Or Thermal Stress On The Two Major Cell Types Of The Blastocyst Stage Murine Embryo., Joe Steve Capehart

LSU Historical Dissertations and Theses

The first objective of this investigation was to determine if either X-irradiation or thermal stress exerted differential effects on the two major cell types of the blastocyst stage murine embryo. Results of this study indicate that X-irradiation dosages of 0 rads, 500 rads, 600 rads, 700 rads, 800 rads, 900 rads or 1000 rads had no effect on the ability of treated embryos to either hatch or develop into trophoblastic outgrowths (P $<$.05). In contrast, X-irradiation treatment over the levels tested did have a detrimental effect on the ability of embryos to develop into trophblastic outgrowths possessing an ICM region (P $>$.05). X-irradiation over the levels tested had no effect on the mean two-dimensional outgrowth surface areas ($\mu$m$\sp2$) (P $>$.05). However, X-irradiation was found to have a …

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Study On Two Chitin-Binding Proteins From Vibrio Parahaemolyticus., Otto Silva Gildemeister Jan 1991

Study On Two Chitin-Binding Proteins From Vibrio Parahaemolyticus., Otto Silva Gildemeister

LSU Historical Dissertations and Theses

Two chitin-binding proteins from Vibrio parahaemolyticus are secreted into the culture medium after the addition of chitin or oligomers of N-acetylglucosamine, but only to lower levels of expression with a few other saccharides. The first protein exhibited a very salt-resistant chitinase activity with activity maxima at pH 6 and 9. The enzyme is stable for 30 minutes at 40$\sp\circ$C, and maximal activity is reached at 50$\sp\circ$C. The chitinase hydrolyzes optimally chitin oligomer substrates with degree of polymerization of 4 and higher. The hydrolytic activity upon the chitotrimer is low; the dimer cannot be hydrolyzed by the enzyme. When incubated with …

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An Analysis Of Educational Films: An African-American Perspective. (Volumes I And Ii)., Brenda Garibaldi Hatfield Jan 1991

An Analysis Of Educational Films: An African-American Perspective. (Volumes I And Ii)., Brenda Garibaldi Hatfield

LSU Historical Dissertations and Theses

Historically African-Americans have been marginalized and subordinated in critical discourse and research in curriculum studies and educational media. There is a vital need for research directed toward African-Americans as subjects and viewers of media used in school curricula. Cultural theorists in curriculum and educational media studies are interested in knowing how social, political and ideological messages are produced and circulated in society, and how student audiences use them to make sense of their experiences. The purpose of this research is to contribute to a better understanding of how racial representations and social identities are coded and produced in educational films, …

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Characterization And Potential Utilization Of Proteases From The Hepatopancreas Of Crawfish, Procambarus Clarkii., Hyeung-Rak Kim Jan 1991

Characterization And Potential Utilization Of Proteases From The Hepatopancreas Of Crawfish, Procambarus Clarkii., Hyeung-Rak Kim

LSU Historical Dissertations and Theses

Four electrophoretically homogenous anionic trypsins, tentatively named trypsin A, B, C, and D, were isolated from crawfish hepatopancreas. Purity was increased 56, 100, 87, and 64-fold with approximately 2.4, 8.1, 5.1, and 3.0% yield for trypsin A, B, C, and D, respectively. The molecular weights of trypsin A, B, C, and D were estimated to be 23,800, 27,900, 24,800, and 31,400, respectively, using Sephacryl S-200 gel filtration. Isoelectric points and amino acid profiles of the trypsins were similar. Caseinolytic activity was consistently highest between pH 5.5 and 10.0 for all trypsins. Amidolytic and esterolytic activities were maximum in a narrower …

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Louisiana Agriculture Magazine, Winter 1991-1992, Lsu Agricultural Experiment Station Jan 1991

Louisiana Agriculture Magazine, Winter 1991-1992, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

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Lsu General Catalog 1991-1992, Louisiana State University And Agricultural And Mechanical College Jan 1991

Lsu General Catalog 1991-1992, Louisiana State University And Agricultural And Mechanical College

LSU General Catalog

The LSU General Catalog describes all undergraduate and graduate departments and programs with degree requirements and courses offered for each one. The General Catalog includes information on registration and financial aid as well as academic services offered to all students.

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A Multivariate Analysis Of Voter Turnout In Louisiana Contested Nonpartisan Judicial Elections, 1981-1988., Olethia Davis Jan 1991

A Multivariate Analysis Of Voter Turnout In Louisiana Contested Nonpartisan Judicial Elections, 1981-1988., Olethia Davis

LSU Historical Dissertations and Theses

The purpose of this dissertation is to determine what factors account for variations in voter turnout in Louisiana contested nonpartisan trial and intermediate appellate court elections. The central questions to be addressed are: (1) what is the precise relationship between incumbency or prior judicial experience, campaign spending, and the race of judicial candidates, ceteris paribus (with all else remaining the same), on electoral participation in judicial elections in the State of Louisiana from 1981 to 1988 and (2) is the electorate that participates in judicial elections typical or atypical of the electorate that participates in presidential general races in terms …

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A Syntactic Language Computer Analysis Of Depressed Versus Non-Depressed Females., Daniel Merton Christy Jan 1991

A Syntactic Language Computer Analysis Of Depressed Versus Non-Depressed Females., Daniel Merton Christy

LSU Historical Dissertations and Theses

This study sought to examine the interrelationship of cognition and language in the clinical syndrome of depression. It was proposed that the types of cognitive differences in depression proposed by Beck and other cognitive theorists would be reflected in language usage, and especially in syntax. Using the Beck Depression Inventory (BDI), subjects were classified as either depressed (BDI $\geq$ 16) or non-depressed (BDI $\leq$ 4). Subjects were asked to speak into a tape recorder for 3-5 minutes on each of three stimuli, a positive event, a negative event, and a neutral opinion stimulus. The language samples were then analyzed using …

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The Introduction Into Tobacco Plants Of Genes Which Encode Some Of The Natural Components Of The Humoral Immune Response Of Hyalophora Cecropia., Luis Julio Cesar Destefano Beltran Jan 1991

The Introduction Into Tobacco Plants Of Genes Which Encode Some Of The Natural Components Of The Humoral Immune Response Of Hyalophora Cecropia., Luis Julio Cesar Destefano Beltran

LSU Historical Dissertations and Theses

A number of plant pathogenic bacteria has been shown to be highly sensitive to SB-37, a derivative of cecropin B (one of the three cecropins from the giant silk moth Hyalophora cecropia). The lytic activity exhibited by SB-37 has been shown to increase up to twenty-fold in the presence of chicken egg white lysozyme. The cDNAs genes encoding SB-37, attacin E (another component of the humoral response of Hyalophora cecropia), and chicken lysozyme have been introduced into tobacco (var Xanthi) and potato (a new cultivar 86007) plants by Agrobacterium-mediated transformation using co-integrate and binary vectors. The genes were placed under …

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The Immunoepidemiology Of Lung Cancer And Smoking: Lymphocyte Subsets., Joan Peters Blackmon Jan 1991

The Immunoepidemiology Of Lung Cancer And Smoking: Lymphocyte Subsets., Joan Peters Blackmon

LSU Historical Dissertations and Theses

The relative proportions of lymphocyte subsets in the peripheral blood constitute a measure of immune status. Results of research to establish whether or not individuals with lung cancer differ in their lymphocyte composition have conflicted due to failure to control for factors other than cancer that affect lymphocyte balance. This study was designed to quantitatively estimate the effects of variables such as disease (lung cancer), smoking, age, circadian and circannual rhythms, and dietary factors on lymphocyte subsets. Questionnaires were given to 64 white males with primary lung cancer and 219 cancer-free white males and a blood sample was obtained. Lymphocytes …

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