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Capillary And Microdevice Electrophoretic Tools For Genetic Analyses: Heteroduplex Analysis For Tuberculosis Drug Susceptibility And Ligase Detection Reaction For Colorectal Cancer Detection, Gloria Thomas

LSU Doctoral Dissertations

The traditional format of electrophoresis, the slab gel, is quickly being replaced by capillary and microdevice platforms, which offer improvements in cost, resolution, speed, quantitation and automation in genetic analyses. These techniques also employ a variety of separation matrices while the slab gel is limited to agarose and polyacrylamide. The research presented here explores the use of these electrophoretic formats for the detection of single nucleotide mutations using two genetic models associated with human disease. Slab gel based heteroduplex analysis (HDA), a popular mutation scanning method, uses a specially designed universal heteroduplex generator (UHG) containing controlled variation to enhance the …

Synthesis And Study Of New Materials For The Visual Detection Of Biomolecules, Ming He

LSU Doctoral Dissertations

The visual determination of saccharides has been of interest for well over a century. Sugars are a relatively challenging class of compounds to analyze. They exhibit great structural similarity as well as transparency in the visible region. The sensing of specific saccharides could aid the monitoring of disease or industrial fermentation products. Herein I present new methods allowing for simple and rapid visual detection of sialic acid, fructose and a homologous series of prototypical neutral oligosaccharides, the linear maltodextrins, containing up to seven glucose residues. The new methods allow for selective color detection of fructose in a large excess of …

Production Of Transgenic Goats By Somatic Cell Nuclear Transfer, Brett C. Reggio

LSU Doctoral Dissertations

A series of experiments were conducted to produce transgenic goats by somatic cell nuclear transfer (NT). In all experiments, donor cells were electrically fused to enucleated metaphase II oocytes, then chemically activated. In a preliminary study to evaluate embryonic development following NT with proliferating or quiescent fibroblast or cumulus cells, significantly more embryos reconstructed with quiescent cumulus cells fused (77%) compared with proliferating cumulus cells (41%), proliferating fibroblasts (36%) or quiescent fibroblasts (37%). Improved development to the eight- to sixteen-cell stage was observed when fibroblast cells were serum starved (serum starved 39% vs. serum fed 15%). However, there was no …

Body And Soul: Food, The Female (In) Corporeal, And The Narrative Effects Of Mind/Body Duality, Andrea Adolph

LSU Doctoral Dissertations

This study combines philosophical, historical, and cultural modes of inquiry in order to explore what has occurred when selected authors have attempted to "write the body." Augmented by archival and primary cultural research, the dissertation is grounded in the experiential, "everyday" qualities of women's lives. Samples of women's cultural materials such as beauty, cookery, and household management texts, and popular women's magazines serve as informative backdrops for an investigation of middle- and working-class British and Anglo-Irish women's culture during the twentieth century. This study investigates some of the ways in which women have thought about food in relation to more …

Doing Homework: Negotiations Of The Domestic In Twentieth-Century Novels Of Teaching, Margaret M. Watson

LSU Doctoral Dissertations

In this project, I analyze seven twentieth-century novels of teaching in order to investigate how notions of “home” and “school” are constructed, connected, and perpetuated in popular teaching narratives. Images of teachers in much of this century’s fiction often rest on views of the school as home that are derived from stereotypes of gender, race, and nationality—stereotypes that can be both inaccurate and repressive. For this reason, I examine these texts in light of how they negotiate school space with domestic space (“domestic” both as personal or familial, and as public or national). I contend that many of these narratives …

Effects Of A Screening Instrument And Parent Handouts On Physicians Recognition And Intervention Of Childrens Behavioral And Emotional Problems, Heather Rech Applegate

LSU Doctoral Dissertations

This study examined the effects of a screening instrument and parent handouts on pediatric residents' recognition and intervention of children's behavioral and emotional problems. Four pediatric residents and 52 parent-child dyads attending a pediatric primary care clinic participated in the study. A multiple baseline design across residents was used. The effects of the interventions were assessed by measuring ten target behaviors of the pediatric residents. After being trained to use the screening instrument, residents' increased the number and variety of questions they asked regarding behavioral and emotional issues. Attempts at intervention by the residents showed small but reliable increases when …

Pathogenic Mechanisms Of Photobacterium Damselae Subspecies Piscicida In Hybrid Striped Bass, Ahmad A. Elkamel

LSU Doctoral Dissertations

Photobacterium damselae subspecies piscicida, previously known as Pasteurella piscicida, is an important pathogen of hybrid striped bass and many fish species cultured in brackish water in the United States, Japan, Europe, and the Mediterranean. The purpose of this study is to investigate virulence mechanisms that contribute to the pathogenesis of this organism. The ability of P. damselae to survive/replicate within hybrid striped bass macrophages was evaluated with an in vitro killing assay. Results indicated that the numbers of bacteria recovered from macrophages at 3, 6, 12, and 18 hours of incubation increased significantly over time. In contrast, the numbers of …

Relationship Between Weight Loss And Body Image In Obese Individuals Seeking Weight Loss Treatment, Deborah Lynn Reas

LSU Doctoral Dissertations

The present study investigated the nature and extent of changes in body image following weight loss treatment in an obese sample and examined the role of weight loss in predicting body image improvement. Participants were 53 obese individuals (BMI > 30) recruited from the Pennington Biomedical Research Center in Baton Rouge, the Scripps Clinic in San Diego, CA, and the Weight Management Center at the St. Charles Hospital in New Orleans. Measures of psychological functioning and a figural body image rating procedure (Body Image Assessment for Obesity; BIA-O, Williamson, et al., 2000) were administered at baseline and after a 6-month follow-up …

Synthesis And Properties Of Novel Macrocycles And Related Chromophoric Benzenoid Compounds, Jorge O. Escobedo Cordova

LSU Doctoral Dissertations

The synthesis and properties of some macrocyclic compounds of interest are presented. Resorcinarenes with functionalized conjugated rigid rods made of oligo-p-phenylenes would lead to an entirely new class of functional, soluble, well-defined architectures impacting a variety of multidisciplinary research efforts. The general synthetic design described in this document could be readly extrapolated to include other electronic systems such as heteroaromatics and polyenes. An extensive collaborative study of chromophore formation in resorcinarene solutions is also described here. The study reveals that upon oxidation, the reversible conversion to acyclic precursors occur first, and then these precursors undergo oxidation to quinoid-type compounds (xanthenes). …

Neo-Onnagata: Professional Cross-Dressed Actors And Their Roles On The Contemporary Japanese Stage, William Hamilton Armstrong Iv

LSU Doctoral Dissertations

Neo-Onnagata: Professional Cross-dressed Actors and Their Roles on the Contemporary Japanese Stage explores the representation of male and female gender in the contemporary Japanese theatre. I particularly discuss a specialized subset of Japanese actor: the neo-onnagata, a contemporary theatre counterpart to Japan's highly stylized classical kabuki tradition of cross-dressed representation. This dissertation represents my attempt to provide these basic aims: to situate the contemporary Japanese cross-dresser in Japanese tradition, to show how cross-dressing acts as a sharp social commentary and mirror, and to introduce some little-represented cross-dressing actors of the contemporary Japanese stage to the academic community at large. In …

The Effects Of Local Hydrodynamics On Mass Transfer In Disordered Porous Media, Gang Guo

LSU Doctoral Dissertations

Interfacial mass transfer in disordered media was studied experimentally and numerically. The dissolution of solid benzoic acid spheres in packed columns showed the existence of spatial variations in mass transfer coefficients in monodisperse and polydisperse packings at the same overall Peclet number. The concept of a local Peclet number (single-particle average) was introduced to quantify the effect of local hydrodynamics on local mass transfer. Correlations between Sherwood number and Peclet number having the form Sh_i=A·(x_iPe)^m were used to quantify data from various sites in each packing. These experiments also showed that the exponent m varies …

Perceptions Of Active Graduate Faculty At A Research Extensive University Regarding Electronic Submission Of Theses And Dissertations (Etds), Ursula Irene Anna Goldsmith

LSU Doctoral Dissertations

This study investigates and explores faculty perceptions toward Electronic Theses and Dissertations (ETDs) during the implementation of ETDs at a university located in the southern portion the United States. Louisiana State University and Agriculture and Mechanical College (LSU) is the flagship university for the state of Louisiana and one of only 25 universities nationwide holding both land-grant and sea-grant status. The Carnegie Foundation for the Advancement of Teaching classified LSU as Doctoral/Research Universities-Extensive. Chapter 1 provides the rationale for this study, the importance of higher education, the importance and role of the faculty advisor in graduate education as influencing the …

Television And Video Game Violence: Age Differences And The Combined Effects Of Passive And Interactive Violent Media, Kelly Stephen Meyers

LSU Doctoral Dissertations

The present research examined the combined effects of violent video games and violent TV programs on third and sixth-grade boys’ thoughts and behavior. In individual sessions, demographic information about the children’s television viewing and video game playing habits was collected. Participants were exposed to one of six following media conditions for 15 minutes; a) watch a violent (wrestling) or a non-violent video segment (basketball), b) play a violent or a non-violent game, or c) watch a violent or non-violent video segment and then play a video game containing the same characters and content. The potential for violent media to prime …

Numerical Study Of Water Coning Control With Downhole Water Sink (Dws) Completions In Vertical And Horizontal Wells, Solomon Ovueferaye Inikori

LSU Doctoral Dissertations

Approximately 2.5 billion dollars is spent annually to solve the problem of produced water in oil and gas wells. Downhole Water Sink (DWS) technology is one industry solution to control water coning in oil wells. DWS technology involves the segregated production of oil and water through separate completions with zonal isolation packer. However, several problems have been experienced in the application of the technology in watered-out oil wells. This study identified two factors that could aid in a better modeling of the technology in old vertical wells – inclusion of capillary transition pressures and relative permeability hysteresis. It also identified …

Violence And The Scapegoat In American Film: 1967-1999, Paul E. Graham Iii

LSU Doctoral Dissertations

This study addresses the proliferation of cinematic violence since the demise of the MPAA’s Production Code in 1966. Bonnie and Clyde and The Wild Bunch were films that projected violence to comment on the civil fervent caused by the Vietnam War. Yet the floodgates these films opened allowed for virtually unlimited and graphic displays of bloodshed to redden big screens for the next three decades. Using the theories of René Girard, namely the scapegoating motif, this study proposes readings of film that, through cinematic ambiguity, contain humanitarian statements against violence by examining the consequences of using force to cause pain. …

Hypoxanthine Phosphoribosyl Transferase And Early Embryo Development, Michael Gerard Collins Ii

LSU Doctoral Dissertations

The objectives of the first experiment were to ascertain if there were any differences between two mouse areola and adipose tissue cell lines. These cell lines differed only in the presence or absence of the hypoxanthine phosphoribosyl transferase (HPRT) gene. It was found that the cell lines were similar for all parameters. The objectives of the second experiment were to determine if the HPRT gene had an effect on the development of preimplantation mouse embryo. To test this transgenic mouse lines were created. The embryo sex and genotype ratio, the embryo diameter, and the embryo developmental patterns, using a Collin-Ryan …

Numerical Studies Of Reactive Polymer Flows In Porous Materials, Honggao Liu

LSU Doctoral Dissertations

This dissertation presents a new numerical model of reactive polymer flow in heterogeneous porous media. A moment representation of the log-normal polymer molecular weight distribution is used to model polymer as a multi-component species. Three leading moments are used to simulate the polymer transport and reaction processes in a two-dimensional porous medium. A new operator splitting technique that allows the moment equations for polymerization to be incorporated into a finite-difference transport model is developed. The novelty of this approach is the use of two different dependent variables (for the transport versus reaction parts of the problem). It is significant from …

Solute Transport In Layered And Heterogeneous Soils, Liuzong Zhou

LSU Doctoral Dissertations

Better understanding of transport of dissolved chemicals in soils and aquifers is important to evaluate and remediate contaminated soils and aquifers. Because of the nature of heterogeneity of field porous media, studies on transport processes in non-homogeneous media are necessary. In this study, transport of solutes in layered and heterogeneous media was investigated using numerical approximations. For layered soils, transport properties were assumed homogeneous within individual layers but different between layers. For heterogeneous systems, either a time-dependent or distance-dependent dispersivity was considered to represent the effects of heterogeneity. In a series of simulations of transport in two-layered soils, we found …

Subversive Aspects Of American Musical Theatre, Donald Elgan Whittaker Iii

LSU Doctoral Dissertations

Critical discourse regarding musical theatre takes, for the most part, the form of a profound silence, due presumably to a dismissal of the genre as simplistic and insubstantial. Not only have the elements of musical theatre been present in the majority of theatrical history, but many of the greatest theories regarding theatre have included these elements, including Brecht and Wagner. Musicals have also often concerned themselves with the Other, centering and sympathizing him/her in a manner unavailable to non-musical works. The Others that have thus been positioned are often delineated from hegemonic groups which are concretely those in power, but …

The Effects Of Songs In The Foreign Language Classroom On Text Recall And Involuntary Mental Rehearsal, Claudia Smith Salcedo

LSU Doctoral Dissertations

This study investigated the effect of music on text recall and involuntary mental rehearsal (din) with students from four college-level Beginning Spanish classes. Two groups heard texts as songs, one group heard the same texts as speech, and one group was the control group. For the text recall variable, a cloze test was administered at the end of each song treatment to determine total words recalled. Students from one of the music groups heard the melody of the song while testing. For the din variable, students were asked to report on the amount of this phenomenon experienced. Data was collected …

Delayed Phytotoxicity Syndrome In Louisiana Rice Caused By The Use Of Thiobencarb Herbicide, Chiliang Chen

LSU Doctoral Dissertations

Thiobencarb (TB), widely used for the control of broadleaf weeds, grasses, and sedges in rice fields, is considered safe for rice plants when used at recommended rates. TB's reductive dechlorination product, dechlorinated thiobencarb (DTB), is highly toxic to rice. TB is naturally transformed into DTB in field soils in certain areas in Japan and the United States. The resultant syndrome is called delayed phytotoxicity syndrome (DPS). This research was conducted to characterize DPS in Louisiana, to compare the toxicity of TB and DTB to rice, to determine uptake and retention rates of TB and DTB by rice, to confirm that …

Control Of Two Perennial Grasses In Southern Turfgrasses, Ronald Eugene Strahan

LSU Doctoral Dissertations

Knotgrass (Paspalum distichum L.) and torpedograss (Panicum repens L.) are perennial grasses that are extremely difficult to remove in established turfgrass. Studies were conducted to determine the potential for controlling these troublesome perennial weeds. Pot studies were conducted for two years to evaluate several herbicides with multiple modes of action for knotgrass control. In graminicides studies, 8 weeks after initial application (WAI) single or split applications of fluazifop or quizalofop provided excellent control (88 to 95%). In contrast, single or split applications of fenoxaprop at 0.21 kg ha-1 and single applications of diclofop at 0.84 kg ha-1 were no better …

Origin Of Lateral Variation Of Seismic Wave Velocities And Density In The Deep Mantle, Shun Ichiro Karato, Bijaya B. Karki

Faculty Publications

Strong constraints can be placed on the origin of heterogeneity of seismic wave velocities and density if the observed ratios of various parameters are compared with mineral physics predictions. They include the shear to compressional wave velocity heterogeneity ratio, R ≡ δ log V / δ log V , the bulk sound to shear wave velocity heterogeneity ratio, R ≡ δ log V / δ log V , and the density to velocity heterogeneity ratio, R ≡ δ log ρ / δ log V . Using mineral physics considerations, we calculate these ratios in the lower mantle corresponding to the …

F01rs Sgb No. 21 (Election Code), Abbott, Fisher

Student Senate Enrolled Legislation

A BILL

To amend Article III, Section 7 of the Student Government Election Code as it relates to active campaigning and recognized student organizations

Louisiana Agriculture Fall, 2001, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Faculty Senate Resolution 02-01, Louisiana State University And Agricultural & Mechanical College

LSU Faculty Senate Publications

Faculty Senate Resolution acknowledging and thanking the Governor and members of the Louisiana Legislature for their support of higher education in the last legislative session, adopted on September 7, 2001.

An Α-Helical Domain Within The Carboxyl Terminus Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoprotein B (Gb) Is Associated With Cell Fusion And Resistance To Heparin Inhibition Of Cell Fusion, Timothy P. Foster, Jeff M. Melancon, Konstantin G. Kousoulas

Faculty Publications

Previous studies from our laboratory indicated that a 28-amino-acid carboxyl-terminal truncation of gB caused extensive virus-induced cell fusion (Baghian et al., 1993, J Virol 67, 2396-2401). We tested the ability of additional truncations and mutations within gB to cause cell fusion in the recently established virus-free cell fusion assay (Turner et al., 1998, J. Virol. 72, 873-875). Deletion of the carboxyl-terminal 28 amino acids of gB (gBΔ28), which removed part of the predicted α-helical structure H17b, caused extensive cell fusion. A gB truncation specified by gBΔ36, which removed the entire H17b domain, caused as much cell fusion as the gBΔ28 …

Measurement Of The Rate Of Ve + D → P + P + E− Interactions Produced By 8B Solar Neutrinos At The Sudbury Neutrino Observatory, Q. R. Ahmad, R. C. Allen, T. C. Andersen, J. D. Anglin, G. Bühler, J. C. Barton, E. W. Beier, M. Bercovitch, J. Bigu, S. Biller, R. A. Black, I. Blevis, R. J. Boardman, J. Boger, E. Bonvin, M. G. Boulay, M. G. Bowler, T. J. Bowles, S. J. Brice, M. C. Browne, T. V. Bullard, T. H. Burritt, K. Cameron, J. Cameron, Y. D. Chan, M. Chen, H. H. Chen, X. Chen

Faculty Publications

Solar neutrinos from 8B decay have been detected at the Sudbury Neutrino Observatory via the charged current (CC) reaction on deuterium and the elastic scattering (ES) of electrons. The flux of νe’s is measured by the CC reaction rate to be φCC(ve) = 1.75 ± 0.07(stat)+0.12−0.11(syst) ± 0.05(theor) × 106 cm−2s−1. Comparison of φCC(ve) to the Super-Kamiokande Collaboration’s precision value of the flux inferred from the ES reaction yields a 3.3σ difference, assuming the systematic uncertainties are normally distributed, providing evidence of an active non-ve component in the solar flux. The total flux of active 8B neutrinos is determined to …

Polymerized Oligopeptide-Surfactant Chiral Micelles, Isiah M. Warner, Eugene J. Billiot, Shahab A. Shamsi, Stefan J. Thibodeaux

College of Science Patents

Chiral separations can be enhanced through the use of polymerized dipeptide-surfactant or oligopeptide-surfactant chiral micelles. Because polymerized micelles eliminate much of the complex dynamic behavior associated with conventional micelles, polymerized chiral micelles have stronger chiral recognition properties than do otherwise-identical, "conventional" or non-polymerized chiral micelles. Recovery of chiral ligands from polymerized chiral micelles is often easier, as the chiral ligands may typically be recovered by simple extraction with an appropriate organic solvent. By contrast, recovering the solute from a conventional, non-polymerized micellar medium by extraction with an organic solvent frequently results in the formation of troublesome emulsion systems. Polymerized chiral …

Internal Parasites Of Cattle In Louisiana And Other Southern States (Research Information Sheet #104), James C. Williams, Alvin F. Loyacano

LSU AgCenter Research Information Sheets

Cattle of all ages, but particularly young cattle, are affected by a diversity of internal parasites. This publication includes information on the different types and effects of treatments.