Digital Commons Network^™

Sperm Cryopreservation Of A Live-Bearing Fish, The Platyfish Xiphophorus Couchianus, Changjiang Huang, Qiaoxiang Dong, Terrence R. Tiersch

Faculty Publications

The objectives of this study were to evaluate the effects of cryoprotectant, osmotic pressure, cooling rate, equilibration time, and sperm-to-extender ratio, as well as somatic relationships of body length, body weight, and testis weight to sperm density in the platyfish Xiphophorus couchianus. Sperm motility and survival duration after thawing were significantly different between cryopreservation with dimethyl sulfoxide (DMSO) and glycerol, with the highest motility at 10 min after thawing obtained with 14% glycerol. With subsequent use of 14% glycerol as cryoprotectant, the highest motility after thawing was observed with Hanks' balanced salt solution (HBSS) across a range of 240-300 mOsm/kg. …

Macrocycle-Derived Functional Xanthenes And Progress Towards Concurrent Detection Of Glucose And Fructose, Oleksandr Rusin, Onur Alpturk, Ming He, Jorge O. Escobedo, Shan Jiang, Fareed Dawan, Kun Lian, Matthew E. Mccarroll, Isiah M. Warner, Robert M. Strongin

Faculty Publications

The detection of saccharides in biological media is of great current importance for the monitoring of disease states. We have previously reported that solutions of boronic acid-functionalized macrocycles form acyclic oligomeric materials in situ. The oligomers contain fluorescent xanthene moieties. Current efforts are aimed at modulating the spectroscopic responses of these materials for the analysis of specific sugars. We describe conditions whereby the xanthene boronic acids exhibit high colorimetric fructose selectivity. In contrast, at physiological levels selective glucose monitoring can be achieved via fluorescence. Additionally, we describe a method which exhibits promise for detecting both glucose and fructose at dual …

The Chlamydomonas Reinhardtii Proteins Ccp1 And Ccp2 Are Required For Long-Term Growth, But Are Not Necessary For Efficient Photosynthesis, In A Low-Co2 Environment, Steve V. Pollock, Davey L. Prout, Ashley C. Godfrey, Stephane D. Lemaire, James V. Moroney

Faculty Publications

The unicellular green alga Chlamydomonas reinhardtii acclimates to a low-CO2 environment by modifying the expression of a number of messages. Many of the genes that increase in abundance during acclimation to low-CO2 are under the control of the putative transcription factor Cia5. C. reinhardtii mutants null for cia5 do not express several of the known low-CO2 inducible genes and do not grow in a low-CO2 environment. Two of the genes under the control of Cia5, Ccp1 and Ccp2, encode polypeptides that are localized to the chloroplast envelope and have a high degree of similarity to members of the mitochondrial carrier …

The Most Fearful Ordeal: Original Coverage Of The Civil War, William D. Bushnell

Civil War Book Review

The Big Apple's perspective

News from the frontlines

Louisiana Agriculture Summer, 2004, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Annotations, Cwbr_Editor

Civil War Book Review

No abstract provided.

Halfbreed: The Remarkable True Story Of George Bent Caught Between The Worlds Of The Indian And The White Man, Barbara Cloud

Civil War Book Review

Culture clash

The Cheyenne and Manifest Destiny

Sperm Cryopreservation Of Green Swordtail Xiphophorus Helleri, A Fish With Internal Fertilization, Changjiang Huang, Qiaoxiang Dong, Ronald B. Walter, Terrence R. Tiersch

Faculty Publications

Sperm cryopreservation for fishes with internal fertilization is essentially unexplored although many species of these fishes are valuable biomedical research models. To explore methods for sperm cryopreservation within the live-bearing genus Xiphophorus, this study used X. helleri to evaluate the effects of cryoprotectant, osmotic pressure, cooling rate, equilibration time, and sperm-to-extender ratio. Sperm motility and survival duration after thawing showed significant differences among different cryoprotectants with the highest motility at 10min after thawing obtained with 14% glycerol. With subsequent use of 14% glycerol as the cryoprotectant, the highest motility after thawing was observed with Hanks' balanced salt solution (HBSS) at …

Memory In Black And White: Race, Commemoration, And The Post-Bellum Landscape, Paul A. Shackel

Civil War Book Review

Interview with Dr. Paul A. Shackel

by Leah Wood Jewett

Measurements Of Compton Scattered Transition Radiation At High Lorentz Factors, Gary L. Case, P. Parker Altice, Michael L. Cherry, Joachim Isbert, Donald Patterson, John W. Mitchell

Faculty Publications

X-ray transition radiation can be used to measure the Lorentz factor of relativistic particles. Standard transition radiation detectors (TRDs) typically incorporate thin plastic foil, foam, or fiber radiators and gas-filled X-ray detectors, and are sensitive up to γ ∼ 104. To reach Lorentz factors up to γ ∼ 105, thicker, denser radiators can be used, which consequently produce X-rays of harder energies (≳ 100 keV). At these energies, scintillator detectors are more efficient in detecting the hard X-rays, and Compton scattering of the X-rays out of the path of the particle becomes important. The Compton scattering can be utilized to …

Identification Of A New Chloroplast Carbonic Anhydrase In Chlamydomonas Reinhardtii, Mautusi Mitra, Scott M. Lato, Ruby A. Ynalvez, Ying Xiao, James V. Moroney

Faculty Publications

Carbonic anhydrases (CA) are zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. The three evolutionarily unrelated families of CAs are designated α-, β-, and γ-CA. Aquatic photosynthetic organisms have evolved different forms of CO2 concentrating mechanisms (CCMs) to aid Rubisco in capturing CO2 from the surrounding environment. One aspect of all CCMs is the critical roles played by various specially localized extracellular and intracellular CAs. Five CAs have previously been identified in Chlamydomonas reinhardtii, a green alga with a well-studied CCM. Here we identify a sixth gene encoding a β-type CA. This new β-CA, designated Cah6, is distinct from …

The Herpes Simplex Virus Type 1 Ul20 Protein Modulates Membrane Fusion Events During Cytoplasmic Virion Morphogenesis And Virus-Induced Cell Fusion, Timothy P. Foster, Jeffrey M. Melancon, Joel D. Baines, Konstantin G. Kousoulas

Faculty Publications

The herpes simplex virus type 1 (HSV-1) UL20 protein is an important determinant for virion morphogenesis and virus-induced cell fusion. A precise deletion of the UL20 gene in the HSV-1 KOS strain was constructed without affecting the adjacent UL20.5 gene. The resultant KOS/UL20-null virus produced small plaques of 8 to 15 cells in Vero cells while it produced wild-type plaques on the complementing cell line G5. Electron microscopic examination of infected cells revealed that the KOS/UL20-null virions predominantly accumulated capsids in the cytoplasm while a small percentage of virions were found as enveloped virions within cytoplasmic vacuoles. Recently, it was …

Simple Entanglement Measure For Multipartite Pure States, Feng Pan, Dan Liu, Guoying Lu, J. P. Draayer

Faculty Publications

A simple entanglement measure for multipartite pure states is formulated based on the partial entropy of a series of reduced density matrices. Use of the proposed new measure to distinguish disentangled, partially entangled, and maximally entangled multipartite pure states is illustrated. © 2004 Springer Science+Business Media, Inc.

S04rs Sgb No. 30 (Roo), Lobianco, Bergeron

Student Senate Enrolled Legislation

A BILL

To Amend the LSU A&M Student Senate Rules of Order to provide for an alternative means of selecting legislative staff

Louisiana Agriculture Spring, 2004, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Feature Essay, John Carlevale

Civil War Book Review

Wildfires of History:

The transforming power of battle

Crofton's Fire, Thomas Dyja

Civil War Book Review

Shell shocked:

A 19th century man's disillusionment with warfare

Pen Of Fire: John Moncure Daniel, Mark R. Cheathem

Civil War Book Review

Diplomacy and journalism:

A life of letters with the Richmond Examiner