High-Throughput Droplet Barcoding And Automated Image Analysis In Microfluidic Droplet Trapping Array, Manibarathi Vaithiyanathan

LSU Master's Theses

Molecularly-targeted therapeutics and personalized medicine have dramatically increased the median survival rate of patients suffering from cancer. However, cellular heterogeneity and the personalized nature of cancer have resulted in the limited success of single drug treatments which has led to the use of multiple therapeutic combinations. This has required the development of new analytical methods capable of multiplexed high-throughput screening (HTS) technologies necessary to identify is single or multi-agent therapies are effective in ex vivo samples like liquid biopsies. Droplet microfluidic devices have garnered significant interest to facilitate high-throughput, single cell analysis of heterogeneous populations. However, these devices are still …

A Study Of The Urban Red Fox (Vulpes Vulpes) Population In Baton Rouge, Louisiana Using Social Media, Ahsennur Soysal

LSU Master's Theses

Foxes are timid yet resourceful animals that are integrated into many urban environments. Because they are elusive, collecting information about the number of urban foxes, their diet and spatial distribution, their interactions with the ecological community in their urban habitat, as well as residents’ response to them, is difficult. Involving stakeholders to participate in the data collection on wildlife via citizen science on social media is one way to overcome this complication, while simultaneously engaging residents in the ecology happening around them. Therefore, we used social media as the platform to engage the public to document and map the foxes …

Expression, Purification And Characterization Of Egf27 From The Notch1 Abruptex Region, Huimin Zhong

LSU Master's Theses

Notch proteins are transmembrane proteins and act as receptors in Notch signaling, which is related to diverse developmental diseases including cancer.^1-2 The mouse Notch1 protein contains 36 tandem epidermal growth factor-like (EGF) repeats in the extracellular domain (ECD).^1-2 Our research project focused on the structural study of the Abruptex region consisting of EGF24-29 in the ECD. The fact that this region is a potential competitor to a Notch ligand, because of its high glycosylation and flexibility, makes it important to solve the three-dimensional structure and investigate the glycosylation effect on the structure of the Abruptex region.³ By …

Allende, Castro, And Pinochet: An Analysis Of Forms Of Address In Political Speeches, Sophie Green

LSU Master's Theses

The central discussion of this thesis concentrates on the uses of ‘we’ and ‘they’ within the corpus of six speeches given by Latin American political leaders in the mid-to-late 20th century. Existing research explains that one’s definition of self as well as a country’s national identity is largely based on the perception of ‘other’ (Costelloe 2014: 322). In other words, the manner in which political speakers discuss the collective ‘we’ and refer to foreign adversaries with the use of ‘they’ can greatly influence national identity. Therefore, the purpose of this study is to analyze the importance of address forms and …

The "Missing Audience": A Query Into The Future Of The Orchestra And The Potential Benefits Of Bringing Live Classical Music To The Community Through Informal Performances, Natalie Wei-Ting Chang

LSU Doctoral Dissertations

In this dissertation, I discuss the traditional organizational model adopted by symphony orchestras in the United States as non-profit arts organizations that are struggling to maintain solvency within the current philanthropic, political, and digital contexts. As part of the discussion, I conduct field research within the local area of the city of Baton Rouge, Louisiana, in providing demonstrations of live and informal classical performance in various businesses and institutions while collecting data via surveys from willing adult participants (ages 18 and above) of all demographics, specifically lower income areas. The survey analysis gives important insights into public perception of symphony …

Mechanistic Modeling Of Nanoparticle-Stabilized Supercritical Co2 Foams And Its Implication In Field-Scale Eor Applications, Doris Patricia Ortiz Maestre

LSU Master's Theses

Previous experimental studies show that nanoparticle-stabilized supercritical CO₂ foams (or, NP CO₂ foams) can be applied as an alternative to surfactant foams, in order to reduce CO₂ mobility in gas injection enhanced oil recovery (EOR). These nanoparticles, if chosen correctly, can be an effective foam stabilizer attached at the fluid interface in a wide range of physicochemical conditions.

By using NP CO₂ foam experiments available in the literature, this study performs two tasks: (i) presenting how a mechanistic foam model can be used to fit experimental data and determine required model parameters, and (ii) investigating the …

Evaluating The Effect Of Chitosan, Water-Soluble Chitosan And Chitosan Application Techniques On The Quality Of Refrigerated Catfish Fillets, Franklin Bonilla Torres

LSU Master's Theses

Chitosan (CH) is a biopolymer synthetized by deacetylation of chitin, it is found in the exoskeleton of crustaceans, cell wall of fungi, and insects. CH’s water insolubility limited the used of this compound in some food systems. However, enzymatic hydrolysis of CH yields water-soluble chitosan (WSC). The aim of this study was to evaluate the effect of chitosan, water-soluble chitosan, and chitosan application techniques on the quality of refrigerated catfish fillets. This research was conducted in two separated studies. In the first study, three solution treatments were prepared: (1) a 1% acetic acid (AA) solution, (2) a 0.5% CH solution …

Producing "Fabulous": Commodification And Ethnicity In Hair Braiding Salons, Sylviane Ngandu-Kalenga Greensword

LSU Doctoral Dissertations

Black women wearing fabulous braids are a striking feature of the Afro-diasporic cultural landscape. However, the braiders and salon owners who enable this aesthetic engineering are seldom acknowledged. This dissertation investigates the experience and role of Caribbean and West and Central African women in the hair braiding industry, a rapidly growing business in the U.S. I address the complexity of these women’s multiple social roles and the multiple consciousness (King, 1988) associated with their demographic characteristics (color, ethnicity, gender, nationality, and immigrant status). The commonalities between the braiders and their mostly African American customers contrast vividly with their perception of …

Paleoenvironmental Reconstruction Using Coral Microatolls (Sp. Siderastrea Siderea) From The Gulf Of Gonâve, Haiti, Gilman Reno Ouellette Jr.

LSU Doctoral Dissertations

Natural and anthropogenic climate changes present unique and serious risks to society. In order to plan and implement adaptation and mitigation strategies successfully, regional manifestations of broad-scale climate changes must be understood robustly. The Caribbean region contains part of the Western Hemisphere Warm Pool (WHWP), a major sea surface temperature (SST) feature that plays a key role in North Atlantic climate. Climate variability in the Caribbean is captured poorly by climate models, due in part to a lack of continuous, multidecadal instrumental climate records from the region. I explored three research questions in this dissertation: Does non-systematic bias exist in …

Electromagnetic Resonant Scattering In Layered Media With Fabrication Errors, Emily Anne Mchenry

LSU Doctoral Dissertations

In certain layered electromagnetic media, one can construct a waveguide that supports a harmonic electromagnetic field at a frequency that is embedded in the continuous spectrum. When the structure is perturbed, this embedded eigenvalue moves into the complex plane and becomes a “complex resonance” frequency. The real and imaginary parts of this complex frequency have physical meaning. They lie behind anomalous scattering behaviors known collectively as “Fano resonance”, and people are interested in tuning them to specific values in optical devices. The mathematics involves spectral theory and analytic perturbation theory and is well understood [16], at least on a theoretical …

Incorporation Of Biorationals And Trap Crop For Stink Bug Management In Soybeans, Kukuh Hernowo

LSU Doctoral Dissertations

Stink bugs are one of the most important seed sucking pests impacting soybean production in Louisiana. Exploration of chemicals that exhibit attraction or repellent activities toward this major pest would be beneficial to develop a push-pull strategy against this pest complex. Spinosad and neem are two commercially available natural insecticides that were claimed to have attractant and repellent activities against stink bugs. To test the potential of these chemicals, a series of experiments was conducted to investigate the olfactory, tactile, feeding, and oviposition preference of stink bugs toward different commercial spinosad products and neem. Additionally, two years of field experiments …

Hpx Smart Executors, Zahra Khatami, Lukas Troska, Hartmut Kaiser, J. Ramanujam, Adrian Serio

Faculty Publications

The performance of many parallel applications depends on loop-level parallelism. However, manually parallelizing all loops may result in degrading parallel performance, as some of them cannot scale desirably to a large number of threads. In addition, the overheads of manually tuning loop parameters might prevent an application from reaching its maximum parallel performance. We illustrate how machine learning techniques can be applied to address these challenges. In this research, we develop a framework that is able to automatically capture the static and dynamic information of a loop. Moreover, we advocate a novel method by introducing HPX smart executors for determining …

Precarious Democracy: "It Can't Happen Here" As The Federal Theatre's Site Of Mass Resistance, Macy Donyce Jones

LSU Doctoral Dissertations

The scholarly consensus of the Federal Theatre Project (FTP) is that it was a massive undertaking set to employ theatre professionals during the Great Depression. That undertaking resulted in vibrant, relevant theatre that helped to build a theatre audience across the nation. Outside of the overview-style scholarship, specialized studies have delved into the FTP as a community-building enterprise, a site of racial/ethnic study, and an essential new play creator.

My scholarship fills a hole that previous FTP scholarship has left open. The FTP was a political machine engaged in producing pro-American propaganda. That aspect of production has been largely left …

Unfriend Me! Applicant Reactions To The Use Of Social Networking Information During The Hiring Process, Byron Shane Lowery

LSU Doctoral Dissertations

In today’s world, it seems everyone has a profile on at least one social networking website. Sites like Facebook, Twitter, and LinkedIn have millions of users. As such, it should come to no surprise that information contained on these sites is being used in various ways. One of the more controversial uses of this information is to screen potential job applicants during the hiring process. Indeed, a growing trend among organizations has been to gather data on applicants in order to identify better employees. However, there is a growing concern about how applicants will react to this practice. Unfavorable reactions …

Teachers' Experience Of A Flood In Their School Community: Their Beliefs, Perceptions, And Thoughts About Practice, Caroline Tolentino

LSU Master's Theses

The increasing number of flooding incidences in Louisiana exposes a significant number of children to the possible traumatic effects of this natural disaster. Flooding takes a toll not only on families and children, but on teachers as well. While the effects of other types of disasters on children have been considered in previous studies, research has not thoroughly addressed the effects of flooding on children and on early childhood teachers. Teachers can be very instrumental in helping young children cope and making sure their needs are met after the experience of a traumatic event (Perry & Szalavitz, 2008; Le Brocque, …

Coupled Plasmon/Molecule Dynamics Near Core-Shell Nanoparticles: Synthesis, Ultrafast Characterization, And Quantum/Classical Modeling, Holden Tyler Smith

LSU Doctoral Dissertations

In this dissertation, the resonance coupling of chromaphoric dyes adsorbed on the surface of plasmonic nanoparticles (NPs) are investigated using a combined theoretical and experi- mental approach. Colloidal gold, gold-silver core-shell, silver-gold core-shell, and gold-silver- gold core-shell-shell NPs are considered. These NPs are thiolated with mercaptosuccinic acid, and malachite green dyes are adsorbed to the surface of the nanoparticle (NP) via elec- trostatic interactions. Second harmonic generation is used to determine the surface to the isotherm of the molecular dyes to the colloidal nanoparticle surface. Adsorption isotherms show that the SHG intensity increases as the concentration of dye increases, reaching …

Biased Genetic Screen Identifies Novel Genes Involved In Antiviral Defense, Tianyun Long

LSU Doctoral Dissertations

ABSTRACT

RNA interference (RNAi) mediates potent antiviral response across kingdoms. In Caenorhabditis elegans nematodes, antiviral RNAi requires a virus sensor that is conserved in mammals and is amplified by secondary small interfering RNAs that are produced in a Dicer-independent manner.

To better understand worm antiviral RNAi, I carried out a biased genetic screen, aiming to identify novel antiviral RNAi genes. To speed up the gene discovery process, the reporter worms used for this genetic screen were engineered to contain extra copies of 4 known antiviral RNAi genes. Therefore, genetic alleles derived from these 4 genes will be automatically rejected during …

The Characterization Of Alpha-Carbonic Anhydrases Through Mutagenesis And Subcellular Localization In Arabidopsis Thaliana, Lance E. Pounds

LSU Master's Theses

Carbonic anhydrases (CAs) are zinc metalloenzymes which catalyze the interconversion of carbon dioxide and bicarbonate. It is hypothesized that CA activity facilitates CO₂ flux in leaf mesophyll cells to maintain optimal rates of photosynthesis or to maintain a pool of inorganic carbon for other metabolic processes. There are three classes of CAs (α, β, and γ) in the model plant Arabidopsis thaliana. The βCAs and γCAs have been well studied, but the αCAs have received relatively little study. There are eight αCA genes in Arabidopsis. This study investigates which αCAs are expressed in shoots, their tissue-specific expression, their …

Incidence Of Aphelenchoides Besseyi In Rice In Louisiana And Host Status Of The Most Widely Planted Cultivars, Felipe Mendes Carvalho Godoy

LSU Master's Theses

Aphelenchoides besseyi, the causal agent of white tip disease of rice, has been considered a minor pest of rice during the past 50 years in the United States. Recently this nematode has been found in a number of quarantine samples in Louisiana and Arkansas. Objectives of this research were to determine incidence of this nematode in commercial seed sold to producers in Louisiana and to determine the host status of major cultivars currently produced in the state. During 2015-2016, a total of 216 seed samples representing 3 medium grain, 18 long grain, and 4 long grain hybrid cultivars were …

A Political Theory Of The Chinese Stock Market, Liang Kong

LSU Doctoral Dissertations

The Chinese stock market crash of 2015 attracted much attention from both the media and academia. Yet it was not a unique incident. The Chinese stock market fluctuates more frequently and drastically than most mature stock markets. The purpose of this work is to explain these unusual stock market fluctuations through a political lens. Traditional financial models and behavioral finance cannot sufficiently explain the unusual fluctuations of the Chinese stock market. Traditional financial models find that economic forces cannot explain all fluctuations in China’s stock market. Behavioral finance attributes the fluctuations to investor’s irrational behavior without explaining why investors behave …

Conflict Reduction Techniques For Gpu Transactional Memory Systems, Sui Chen

LSU Doctoral Dissertations

The continued evolution of GPUs have enabled the use of irregular algorithms which involve fine-grained data sharing between threads, as well as transaction processing applications such as databases. Transactional Memory (TM) is derived from databases, which by itself is a programming construct that simplifies the programming of parallel workloads and combines the advantages of traditional approaches including fine-grained and coarse-grained locking. While hardware support for TM has started to enter mainstream commodity products, it is much farther from becoming reality on the GPU and is still being researched. In this dissertation, we study the challenges for supporting transactional workloads on …

Heat, Acid And Osmotic Stress Tolerance Of Leuconostoc Mesenteroides As Influenced By Prior Exposure To Various Mild Stress Conditions, Ingrid C. Osorio Rodriguez

LSU Master's Theses

The importance of Leuconostoc mesenteroides is recognized for its contribution to taste in cultured dairy products. It has been cited for its potential role as a probiotic. Consumer demand exists for new dairy products with health benefits. In the manufacture of probiotic-products survival of Leuconostoc mesenteroides would depend on its ability to tolerate challenging processing and storage conditions. Improved viability by prior exposure to mild stresses can increase stress tolerance toward a more severe stress. Furthermore, it can result in cross-protection due to connection of several proteins in response to stresses. Objective was to evaluate the effect of prior …

Evaluation Of Winter Management Methods To Enhance Survival Of The Giant Salvinia Weevil, Cyrtobagous Salviniae, Lori Robyn Moshman

LSU Master's Theses

Giant salvinia (Salvinia molesta Mitchell) is a noxious aquatic weed in the southeastern United States. Dense plant mats create ecological and economic consequences by displacing native species and impacting freshwater industries. Biological control programs using the salvinia weevil (Cyrtobagous salviniae Calder and Sands, Coleoptera: Curculionidae) have limited efficacy in giant salvinia’s northernmost range due to the weevil’s low cold tolerance and long-term establishment rates. Spunbonded polypropylene (SBP) fabrics and manipulation of plant density were evaluated as two strategies for managing giant salvinia mats during winter. Greenhouse, laboratory, and field experiments were conducted to determine the suitability of these …

Self-Assembly Mechanisms Of Organosilanes And Porphyrins Investigated With Scanning Probe Microscopy, Phillip Charles Chambers Ii

LSU Doctoral Dissertations

This dissertation details the development of new fabrication strategies for the preparation of spatially selective surfaces by combining techniques of particle lithography and scanning probe microscopy (SPM). This combination of lithography and nanoscale surface characterization was applied to study the mechanisms of molecular level surface-assembly of organosilanes and porphyrin on surfaces of Si(111). Particle lithography was used to investigate the surface assembly of 4-chloromethylphenyltrichlorosilane (CMPS) within exposed sites of nanoholes in selected solvents and at selected temperatures to gain insight into the details of self-polymerization. Nanopillars of CMPS were generated under selected conditions of solvent and temperature and characterized with …

Multigeneration Effects Of Chronic Exposure To Polycyclic Aromatic Hydrocarbons In Gulf Killifish (Fundulus Grandis), Andrea Yammine

LSU Master's Theses

The goal of this study was to examine potential adaptation within two generations of Gulf killifish exposed to polycyclic aromatic hydrocarbons (PAH) while also investigating the use of mucus as an effective and non-lethal alternative to determine concentrations of 11-ketotestosterone (11-KT) and vitellogenin (VTG) of F. grandis. Multiple biomarkers were used to assess adaptation within these fish, such as lysozyme (enzyme critical in innate immunity), and 11-ketotestosterone and vitellogenin (endocrine hormones often affected by PAHs).

Mucal concentrations of 11-KT and VTG were determined and evaluated for effectiveness through comparative assays with blood plasma. This method was then applied to …

Weather Communication On Twitter: Identifying Popular Content And Optimal Warning Format Via Case Studies And A Survey Analysis, Joshua D. Eachus

LSU Doctoral Dissertations

The use of Twitter as a channel for weather information inspires a deeper analysis of key information nodes during episodes of high impact weather, especially local meteorologists. To optimize communication on the channel, it is important to understand what kinds of messages produce exposure and attention among users—which translates to knowledge that could improve the reach of a warning. Literature identifies two key models that well describe the cognitive processing of tweets and warnings. The Protective Action Decision Model (PADM) describes risk perception and the factors that enable or disable one from acting on a warning. Particularly through environmental and …

Perpetual Integral Functionals Of Brownian Motion And Blowup Of Semilinear Systems Of Spdes, Eugenio Guerrero, José Alfredo López-Mindela

Communications on Stochastic Analysis

No abstract provided.

Ethical Leadership: A Study Of Traits And Behaviors Of Leaders In Higher Education Today, Samuel L. Wyatt

LSU Doctoral Dissertations

The primary purpose of this study was to determine the traits and behaviors of an ethical leader as perceived by tenured and tenure-track faculty at a research university (RU/VH) in the southeastern portion of the United States. The researcher utilized a researcher-designed survey instrument which consisted of 44 possible traits and behaviors of an ethical leader and selected demographic characteristics.

Examination of the study results revealed that the majority of respondents were male (66.20%) and tenured (75.00%). Additionally, the largest group were Professors (49.60%), and had been at the study institution for less than l0 years (38.20%). Most of the …

The Stringent Response Induced By Phosphate Limitation Promotes Purine Salvage In Agrobacterium Fabrum, Smitha Sivapragasam, Dinesh K. Deochand, Jacob K. Meariman, Anne Grove

Faculty Publications

© 2017 American Chemical Society. Agrobacterium fabrum induces tumor growth in susceptible plant species. The upregulation of virulence genes that occurs when the bacterium senses plant-derived compounds is enhanced by acidic pH and limiting inorganic phosphate. Nutrient starvation may also trigger the stringent response, and purine salvage is among the pathways expected to be favored under such conditions. We show here that phosphate limitation induces the stringent response, as evidenced by production of (p)ppGpp, and that the xdhCSML operon encoding the purine salvage enzyme xanthine dehydrogenase is upregulated ∼15-fold. The xdhCSML operon is under control of the TetR family transcription …

Interaction Of Cocoa Powder With Intestinal Microbiota, Martha M. Escoto Sabillon

LSU Master's Theses

Cocoa is the fully fermented and dried seed of the cacao tree (Theobroma cacao L.) which has prebiotic properties, due to their high concentration of polyphenols. Therefore, the ingestion of cocoa could cause changes in the proportions of the intestinal microbiota that can influence the intestinal immune response. The objective of this study was to determine the effect of alkalization process of the cocoa bean in the diversity of the gut microbiota. The samples were “lavado” unprocessed cocoa powder, “natural” unprocessed cocoa powder, “D-11-S” as alkalized cocoa powder, “D-11-B” heavily alkalized cocoa powder, and raw cocoa “shells” and a control …