Digital Commons Network^™

Jak/Stat3 Regulated Global Gene Expression Dynamics During Late-Stage Reprogramming Process, Ling Wang, Zongliang Jiang, Delun Huang, Jingyue Duan, Chang Huang, Shannon Sullivan, Kaneha Vali, Yexuan Yin, Ming Zhang, Jill Wegrzyn, Xiuchun Cindy Tian, Young Tang

Faculty Publications

© 2018 The Author(s). Background: The generation of induced pluripotent stem cells (iPSCs) has underdefined mechanisms. In addition, leukemia inhibitory factor (LIF) activated Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) pathway is the master regulator for naïve-state pluripotency achievement and maintenance. However, the regulatory process to attain naïve pluripotent iPSCs is not well understood. Results: We performed transcriptome analysis to dissect the genomic expression during mouse iPSC induction, with or without blocking the JAK/STAT3 activity. We describe JAK/STAT3 signaling-specific biological events such as gametogenesis, meiotic/mitotic cell cycle, and DNA repair, and JAK/STAT3-dependent expression of key transcription factors such …

State Of Crime, 2018: A Look At Crime And Crime Response Needs In East Baton Rouge Parish, Hillar C. Moore Iii

Reports

No abstract provided.

My Year As A Fed, Prosanta Chakrabarty

Faculty Publications

No abstract provided.

Chemotherapeutic Applications Of Rhodamine Based Nanogumbos, Nimisha Bhattarai

LSU Doctoral Dissertations

The work presented in this dissertation employs nanomaterials derived from a group of uniform materials based on organic salts (GUMBOS) for selective chemotherapeutic applications. GUMBOS, similar to ionic liquids, are organic salts consisting of a bulky cationic and anionic moiety. In contrast to ionic liquids, these materials have melting points ranging from 25–250 °C, making them solid phase at room temperature. Similar to ionic liquids, GUMBOS display tunable properties, such as hydrophobicity and solubility, through counter ion variation. These tunable properties provide a variety of applications for these GUMBOS, including selective chemotherapeutics applications. The …

The Amino Terminus Of Herpes Simplex Virus 1 Glycoprotein K (Gk) Is Required For Gb Binding To Akt, Release Of Intracellular Calcium, And Fusion Of The Viral Envelope With Plasma Membranes, Farhana Musarrat, Nithya Jambunathan, Paul J.F. Rider, V. N. Chouljenko, K. G. Kousoulas

Faculty Publications

© 2018 American Society for Microbiology. Previously, we have shown that the amino terminus of glycoprotein K (gK) binds to the amino terminus of gB and that deletion of the aminoterminal 38 amino acids of gK prevents herpes simplex virus 1 (HSV-1) infection of mouse trigeminal ganglia after ocular infection and virus entry into neuronal axons. Recently, it has been shown that gB binds to Akt during virus entry and induces Akt phosphorylation and intracellular calcium release. Proximity ligation and two-way immunoprecipitation assays using monoclonal antibodies against gB and Akt-1 phosphorylated at S473 [Akt-1(S473)] confirmed that HSV-1(McKrae) gB interacted with …

The Mediating Role Of Korean Immigrant Mothers’ Psychological Well-Being In The Associations Between Social Support And Authoritarian Parenting Style, You Jung Seo, Charissa S.L. Cheah, Sevgi Bayram Özdemir, Craig H. Hart, Christy Y.Y. Leung, Shuyan Sun

Faculty Publications

© 2017, Springer Science+Business Media, LLC. We examined the mediating role of Korean immigrant mothers’ psychological well-being in the associations between mothers’ emotional vs. instrumental support received from their kin, and their authoritarian parenting style with their preschoolers using longitudinal data. First-generation Korean immigrant mothers with preschool-aged children (N = 158; Mmaternal age = 36.11 years, SD = 3.90; Mchild age = 4.43 years, SD = 1.10) residing in Maryland, U.S., participated in three assessment waves. Each assessment wave was 6 months apart. Mothers reported on the amount of perceived emotional and instrumental support they received from their kin, their …

Pilot Study Of The Tart Cherry Juice For The Treatment Of Insomnia And Investigation Of Mechanisms, Jack N. Losso, John W. Finley, Namrata Karki, Ann G. Liu, Alfredo Prudente, Russell Tipton, Ying Yu, Frank L. Greenway

Faculty Publications

Background: Insomnia is common in the elderly and is associated with chronic disease, but use of hypnotics increases the incidence of falls. Montmorency tart cherry juice has improved insomnia by self-report questionnaire. Study Question: Is insomnia confirmed by polysomnography and is tryptophan availability a potential mechanism for treating insomnia? Study Design: A placebo-controlled balanced crossover study with subjects older than 50 years and insomnia were randomized to placebo (2 weeks) or cherry juice (2 weeks) (240 mL 2 times/d) separated by a 2-week washout. Measures and Outcomes: Sleep was evaluated by polysomnography and 5 validated questionnaires. Serum indoleamine 2,3-dioxygenase (IDO), …

Unintended Consequences For Tanf: An Incubator For Domestic Violence?, Kathryn Rose Deleo

Honors Capstones

No abstract provided.

Influence Of Cooling Medium On Low Temperature Strength Of Asphalt Binders, Mihai Marasteanu, Augusto Cannone Falchetto, Sreelatha Balamurugan, Ioan I. Negulescu

Faculty Publications

The effect of three different cooling media, ethanol, potassium acetate (PA) and air, on the strength of asphalt binder at low temperature is investigated with a modified Bending Beam Rheometer (BBR) and the Direct Tension Tester (DTT). Similar strength values are obtained in air and PA and significantly lower values are obtained in ethanol. The use of fluorescing markers demonstrates that ethanol diffuses in the asphalt binder, while diffusion does not occur when PA is used. Using size effect theory, BBR strengths in PA and in air are found to be equivalent to DTT strength in PA.

A Practical Beginner's Guide To Cyclic Voltammetry, Noémie Elgrishi, Kelley J. Rountree, Brian D. Mccarthy, Eric S. Rountree, Thomas T. Eisenhart, Jillian L. Dempsey

Faculty Publications

© 2017 The American Chemical Society and Division of Chemical Education, Inc. Despite the growing popularity of cyclic voltammetry, many students do not receive formalized training in this technique as part of their coursework. Confronted with self-instruction, students can be left wondering where to start. Here, a short introduction to cyclic voltammetry is provided to help the reader with data acquisition and interpretation. Tips and common pitfalls are provided, and the reader is encouraged to apply what is learned in short, simple training modules provided in the Supporting Information. Armed with the basics, the motivated aspiring electrochemist will find existing …

Metal-Based Microchannel Heat Exchangers Made By Molding Replication And Assembly, Wen Jin Meng, Fanghua Mei

College of Engineering Patents

Compression molding of metals is used to make microchannel heat exchangers. Heat transfer can be improved by employing controlled microchannel surface roughness. Flux-free bonding is achieved using a eutectic thin-film intermediate layer. Seals are leak-tight, mechanically strong, and uniform across multiple contact areas. The metal heat exchangers may be mass-produced inexpensively, and are useful for applications including the cooling of computer chips and other high-power electronic devices, air conditioning, refrigeration, condenser plates, radiators, fuel cell heat management, and instant water heating.

Reference Quality Genome Assemblies Of Three Parastagonospora Nodorum Isolates Differing In Virulence On Wheat, Jonathan K. Richards, Nathan A. Wyatt, Zhaohui Liu, Justin D. Faris, Timothy L. Friesen

Faculty Publications

Parastagonospora nodorum, the causal agent of Septoria nodorum blotch in wheat, has emerged as a model necrotrophic fungal organism for the study of host-microbe interactions. To date, three necrotrophic effectors have been identified and characterized from this pathogen, including SnToxA, SnTox1, and SnTox3. Necrotrophic effector identification was greatly aided by the development of a draft genome of Australian isolate SN15 via Sanger sequencing, yet it remained largely fragmented. This research presents the development of nearly finished genomes of P. nodorum isolates Sn4, Sn2000, and Sn79-1087 using long-read sequencing technology. RNAseq analysis of isolate Sn4, consisting of eight time points covering …

Multigrid Methods For Saddle Point Problems: Darcy Systems, Susanne C. Brenner, Duk Soon Oh, Li Yeng Sung

Faculty Publications

We design and analyze multigrid methods for the saddle point problems resulting from Raviart–Thomas–Nédélec mixed finite element methods for the Darcy system in porous media flow. Uniform convergence of the W-cycle algorithm in a nonstandard energy norm is established. Extensions to general second order elliptic problems are also addressed.

Rice Nucleosome Patterns Undergo Remodeling Coincident With Stress-Induced Gene Expression, Qi Zhang, Dong Ha Oh, Sandra Feuer Ditusa, Mangu V. Ramanarao, Niranjan Baisakh, Maheshi Dassanayake, Aaron P. Smith

Faculty Publications

© 2018 The Author(s). Background: Formation of nucleosomes along eukaryotic DNA has an impact on transcription. Major transcriptional changes occur in response to low external phosphate (Pi) in plants, but the involvement of chromatin-level mechanisms in Pi starvation responses have not been investigated. Results: We mapped nucleosomes along with transcriptional changes after 24-h of Pi starvation in rice (Oryza sativa) by deep sequencing of micrococcal nuclease digested chromatin and ribosome-depleted RNA. We demonstrated that nucleosome patterns at rice genes were affected by both cis- and trans-determinants, including GC content and transcription. Also, categorizing rice genes by nucleosome patterns across the …

Experimental Evidence Of A Pathogen Invasion Threshold, Tad A. Dallas, Martin Krkošek, John M. Drake

Faculty Publications

© 2018 The Authors. Host density thresholds to pathogen invasion separate regions of parameter space corresponding to endemic and diseasefree states. The host density threshold is a central concept in theoretical epidemiology and a common target of human and wildlife disease control programmes, but there is mixed evidence supporting the existence of thresholds, especially in wildlife populations or for pathogens with complex transmission modes (e.g. environmental transmission). Here, we demonstrate the existence of a host density threshold for an environmentally transmitted pathogen by combining an epidemiological model with a microcosm experiment. Experimental epidemics consisted of replicate populations of naive crustacean …

Evaluation Of Growth Characteristics, Yield, Marketability And Nitrate Levels Of Lettuce (Lactuca Sativa L) Cultivars Produced In South Louisiana, William D. Afton

LSU Master's Theses

Lettuce (Lactuca sativa L.) is an important leafy vegetable crop grown in worldwide food systems with the United States ranking second behind China in total production. In 2009 the USDA Food Economic Research Service reported 1,057,715 hectares of lettuce grown in the world. Lettuce is a common market crop grown across the United States. Nitrate (NO^3-) accumulation in lettuce and other leafy vegetables has been found to be a potential health threat and when consumed by humans, potentially causing methaemoglobinaemia and other diseases. There are four main types of lettuce and numerous cultivars within each type. Fresh …

A Case Study: The Attitudes Of A Teaching Assistant Of French Toward Instructional Technology, Farida Emelia Ngandu Tshiebue

LSU Doctoral Dissertations

Technology has shaped the personal experiences of Teaching Assistants (TAs) of French at the collegiate level in the United States (US), what they feel about the technology tools that are accessible in their work environment, and how they use these tools in their practice of teaching. Yet, there is limited research on the specific topic of the attitudes of TAs of French toward the instructional technologies that are available to them. This study sought to investigate the attitudes of a singular TA of French at a southern US university toward instructional technology (IT) by exploring the dynamics of IT use …

Image-Based Modeling Of Flow Through Porous Media: Development Of Multiscale Techniques For The Pore Level, Timothy Wayne Thibodeaux

LSU Doctoral Dissertations

Increasingly, imaging technology allows porous media problems to be modeled at microscopic and sub-microscopic levels with finer resolution. However, the physical domain size required to be representative of the media prohibits comprehensive micro-scale simulation. A hybrid or multiscale approach is necessary to overcome this challenge. In this work, a technique was developed for determining the characteristic scales of porous materials, and a multiscale modeling methodology was developed to better understand the interaction/dependence of phenomena occurring at different microscopic scales. The multiscale method couples microscopic simulations at the pore and sub-pore scales. Network modeling is a common pore-scale technique which employs …

Biomedical Applications Of Hydrogels And Gumbos, Kelsey Elizabeth Mcneel

LSU Doctoral Dissertations

Sodium deoxycholate (NaDC) is a small bile salt that was used in this dissertation to produce gelation of tris(hydroxymethyl)amino-methane (TRIS) solutions above, below, and near the pKa of NaDC. These solutions respectively yielded a neutral gelator, a charged gelator, and a mixture of each. Impacts of ionic interactions on gel formation were studied in detail and showed that pH can be used to modify many hydrogel properties including sol-gel temperature, crystallinity, and mechanical strength. It was also observed that pH modification of the hydrogels affected nanoparticle formation. Nanoparticles derived from a Group of Uniform Materials Based on Organic Salts (nanoGUMBOS), …

An Evaluation Of The Groundwater Microbial Community's Toluene Biogenesis Potential At A Site In Southeast Louisiana, Michael A. Griffin Jr.

LSU Master's Theses

The broad goal of the research described in this thesis was to better understand the potential for biological toluene production to occur in groundwater at a Superfund Site located in southeast Louisiana. Previous literature reported that bacterial isolates under laboratory test conditions and undefined microbial communities in anaerobic sludge digesters and seasonally stratified lakes can produce toluene biogenically. Most of the research reported on this subject previously, however, has been on systems in Europe and little research has been reported previously on such phenomena in North America.

In the research described here, studies were conducted utilizing groundwater from a Superfund …

Diversionary Behavior During Congestion, Venkata Vamsikrishna Peddisetty

LSU Master's Theses

Most people in the US prefer to travel on freeways, but when there is congestion they divert onto local routes to avoid travel delay. This study measures the traffic diversion induced by an increase in congestion on I-10 freeway in Baton Rouge during congestion, using a Bluetooth Detection System. The main objective of the study was to identify the difference in the level of congestion on the I-10 between the Mississippi River Bridge and the I-10/I-12 split and the level of congestion on local roads that triggers traffic to divert from the freeway to parallel arterials. Other objectives include measuring …

Gumbo Yearbook, Class Of 2018, Louisiana State University And Agricultural And Mechanical College

Gumbo Yearbook

The Gumbo yearbook chronicles the entire academic year at LSU. In words and especially photos, the Gumbo shows the people, places, and events that make each year unique. In addition to formal portraits of schools and departments, the book contains hundreds of snapshots of students with their friends and dozens of pages showcasing organizations.

Lsu General Catalog 2018-2019, Louisiana State University And Agricultural And Mechanical College

LSU General Catalog

The LSU General Catalog describes all undergraduate and graduate departments and programs with degree requirements and courses offered for each one. The LSU General Catalog includes information on registration and financial aid as well as academic services offered to all students.

Challenges Of Elephant Conservation: Insights From Oral Histories Of Colonialism And Landscape In Tsavo, Kenya, Peter Ngugi Kamau, Andrew Sluyter

Faculty Publications

No abstract provided.

Baton Rouge Area Violence Elimination: Final Report, Cecile C. Guin, Jada Thomas-Smith, Elizabeth Winchester, Tracey E. Rizzuto, Aimee H. Moles, Steven Maberry, Haojie Zhu, Judith L. Rhodes, Juan J. Barthelemy

Reports

No abstract provided.

Fall 2018, Tom Barber

Civil War Book Review

No abstract provided.

Lincoln's Lieutenants: The High Command Of The Army Of The Potomac, Andrew S. Bledsoe

Civil War Book Review

Inside the Union High Command The Army of the Potomac, arguably the Union's most important field army, has been the subject of many historical examinations. Classic works like Bruce Catton's beautifully-written narrative trilogy and Rusell H. Beatie's analytical multivolume study have, for years, r....

Novel Spiroplasma Spp. Cultured From Brains And Lymph Nodes From Ruminants Affected With Transmissible Spongiform Encephalopathy, Frank O. Bastian, James Lynch, Sue Hagius, Xiaochu Wu, Greg Mccormick, Donald G. Luther, Philip H. Elzer

Faculty Publications

© 2017 American Association of Neuropathologists, Inc. Spiroplasma spp., tiny filterable wall-less bacteria, are consistently associated with the transmissible spongiform encephalopathies (TSE). Spiral forms have been transiently isolated from TSE-affected brain tissues in SP4 growth media designed for isolation of Spiroplasma spp., but the isolate could not be propagated in SP4 media. A bacterium must grow in vitro in cell-free cultures to allow full characterization of a suspect pathogen. Here, a novel Spiroplasma sp. was isolated from scrapie- and chronic wasting disease (CWD)-affected brains and lymph nodes. Filtrates of tissue homogenates inoculated into Brucella media incubated for 14 days at …

Nanotechnology Considerations For Poultry And Livestock Production Systems - A Review, Mohamed I. El Sabry, Kenneth W. Mcmillin, Cristina M. Sabliov

Faculty Publications

© 2018 by Mohamed I. El Sabry. The global animal productivity should proportionally increase to meet the food needs of a growing population. This article presents an overview of the current and promising nano-applications in poultry and livestock production systems that could offer opportunities for improved efficiencies and productivity. Some basic information on nanotechnology and the economics of nanotechnology is provided. Poultry and animal production systems, current situation and available tools and techniques are presented in parallel with animal health care, animal nutrition, animal shetter and food processing nano-applications and their advantages. These applications are directly or indirectly related to …

Sacred Vs. Profane In The Great War: A Neutral’S Indictment, Marty Miller

Faculty Publications

No abstract provided.