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Experimental And Computational Tools For Single Cell Analysis In Cancer Diagnostics, Manibarathi Vaithiyanathan
Experimental And Computational Tools For Single Cell Analysis In Cancer Diagnostics, Manibarathi Vaithiyanathan
LSU Doctoral Dissertations
Substantial evidence shows that cellular heterogeneity commonly exists within an isogenic or clonal population. Whether in isolation or caused through a combination of the above events, cellular heterogeneity can dramatically influence cellular decision making and cell fate, however, this can be masked by the average response from a population. One approach to solve this issue is to analyze a population at the individual cell level. The goal of this work is to develop high-throughput experimental and computational platforms to screen and quantify single cancer cells for specific intracellular enzyme activities. An interdisciplinary approach was taken to 1) better understand the …
Impacts Of The Deepwater Horizon Oil Spill On Reproduction And Ontogeny Of Gulf Killifish (Fundulus Grandis), Charles Alexander Brown
Impacts Of The Deepwater Horizon Oil Spill On Reproduction And Ontogeny Of Gulf Killifish (Fundulus Grandis), Charles Alexander Brown
LSU Doctoral Dissertations
On April 20, 2010, the Deepwater Horizon oil spill released an estimated 779 million liters of Macondo-252 crude oil into the Gulf of Mexico, making it the largest marine oil spill in history. Over a thousand kilometers of marshland that many species of fish use as a spawning grounds and nurseries was oiled, exposing breeding adult fish and their offspring to oil. My dissertation investigates the impacts of the Deepwater Horizon oil spill and associated remediation efforts on the reproduction and ontogeny of a sentinel ecotoxicological species, the Gulf killifish (Fundulus grandis). Concerns were raised regarding use of …
Influence Of Neighboring Clonal-Colonies On Aflatoxin Production By Aspergillus Flavus, Rebecca R. Sweany, Kenneth E. Damann
Influence Of Neighboring Clonal-Colonies On Aflatoxin Production By Aspergillus Flavus, Rebecca R. Sweany, Kenneth E. Damann
Faculty Publications
Aspergillus flavus is an ascomycete fungus that infects and contaminates corn, peanuts, cottonseed, and treenuts with acutely toxic and carcinogenic aflatoxins. The ecological function of aflatoxin production is not well understood; though not phytotoxic, aflatoxin may be involved in resisting oxidative stress responses from infection or drought stress in plants. Observation of aflatoxin stimulation in 48-well plates in response to increasing inoculated wells sparked an investigation to determine if A. flavus volatiles influence aflatoxin production in neighboring colonies. Experiments controlling several culture conditions demonstrated a stimulation of aflatoxin production with increased well occupancy independent of pH buffer, moisture, or isolate. …
A Study Of Particle-Laden Flows From Meso And Micro-Scale Perspectives, Daniel Guedes De Oliveira
A Study Of Particle-Laden Flows From Meso And Micro-Scale Perspectives, Daniel Guedes De Oliveira
LSU Doctoral Dissertations
Particle-laden flows are investigated numerically from a meso-scale perspective using Computational Fluid Dynamics coupled with Discrete Element Method (CFD-DEM) and from a micro-scale perspective using Particle Resolved Direct Numerical Simulation (PR-DNS). For the former, the dynamics of a pseudo-2D pulsed fluidized bed (PFB) consisting of 400,000 to 800,000 particles was investigated (Chapters 2 and 3). The focus is on the capabilities of CFD-DEM to (1) reproduce pattern formation in these systems and (2) further the understanding of the dynamics of PFB's as a function of pulsation parameters. In Chapter 4, a two-spheres system is investigated with a recently implemented PR-DNS …
Concentrations, Sources And Health Risks Of Particulate Matter In Southeast Us: Current Status And Future Changes, Fenglin Han
Concentrations, Sources And Health Risks Of Particulate Matter In Southeast Us: Current Status And Future Changes, Fenglin Han
LSU Doctoral Dissertations
Evaluation of long-term air quality trends and prediction of future air quality may help to design and demonstrate effectiveness of control strategies and guide future air quality management. As one of the six criteria air pollutants (CAPs) regulated by the US Environmental Protection Agency (EPA), particulate matter with aerodynamic diameter of 2.5 µm or less (PM2.5) is of great interests since it has adverse effects on human health, visibility and climate. This study aims to understand the current status and future changes in concentrations, sources, and health risks of PM2.5 in Southeast US. Firstly, observations were analyzed …
Ageism And Embodied Stereotypes: A Study Of Adult Learners In Community College At Midlife, Marla Jane Erwin
Ageism And Embodied Stereotypes: A Study Of Adult Learners In Community College At Midlife, Marla Jane Erwin
LSU Doctoral Dissertations
Adult students are generally classified as a single group for study, yet developmental psychologists recognize separate developmental periods during adulthood that suggest adult students at midlife may experience development within higher education differently that younger adult students, in part due to ageism expressed at individual, institutional and internalized levels. This project applies the concept of lifespan developmental periods to distinguish students at midlife as a focus of inquiry using a mixed method design. Twenty-nine faculty and 205 students responded to the Relating to Older People Evalution (ROPE; Cherry & Palmore, 2008) to assess self-reports of both positive and negative ageist …
Anthropogenic, Direct Pressures On Coastal Wetlands, A Newton, J Icely, S Cristina, G M. Perillo, A Newton, D Ashan, S Cragg, Y M. Luo, C Tu, Y Li, H B. Zhang, R Ramesh, D L. Forbes, C Solidoro, B Bejaoui, S Gao, R Pastres, H Kelsey, D Taillie, N Nhan, A C. Brito, L I. De, K U. R, C
Anthropogenic, Direct Pressures On Coastal Wetlands, A Newton, J Icely, S Cristina, G M. Perillo, A Newton, D Ashan, S Cragg, Y M. Luo, C Tu, Y Li, H B. Zhang, R Ramesh, D L. Forbes, C Solidoro, B Bejaoui, S Gao, R Pastres, H Kelsey, D Taillie, N Nhan, A C. Brito, L I. De, K U. R, C
Faculty Publications
Coastal wetlands, such as saltmarshes and mangroves that fringe transitional waters, deliver important ecosystem services that support human development. Coastal wetlands are complex social-ecological systems that occur at all latitudes, from polar regions to the tropics. This overview covers wetlands in five continents. The wetlands are of varying size, catchment size, human population and stages of economic development. Economic sectors and activities in and around the coastal wetlands and their catchments exert multiple, direct pressures. These pressures affect the state of the wetland environment, ecology and valuable ecosystem services. All the coastal wetlands were found to be affected in some …
Influence Of Macrobenthos (Meretrix Meretrix Linnaeus) On Erosion-Accretion Processes In Intertidal Flats: A Case Study From A Cultivation Zone, B. Shi, P.D. Pratolongo, Y. Du, J. Li, S.L. Yang, J. Wu, K. Xu, Y.P. Wang
Influence Of Macrobenthos (Meretrix Meretrix Linnaeus) On Erosion-Accretion Processes In Intertidal Flats: A Case Study From A Cultivation Zone, B. Shi, P.D. Pratolongo, Y. Du, J. Li, S.L. Yang, J. Wu, K. Xu, Y.P. Wang
Faculty Publications
No abstract provided.
Louisiana Digital Library Collections As Data Intro Survey: Selected Survey Results To Support Reports And Publications, Sl Ziegler, Leah Powell Duncan, Elizabeth Joan Kelly
Louisiana Digital Library Collections As Data Intro Survey: Selected Survey Results To Support Reports And Publications, Sl Ziegler, Leah Powell Duncan, Elizabeth Joan Kelly
Faculty Publications
No abstract provided.
Methods For Broad-Scale Plant Phenology Assessments Using Citizen Scientists’ Photographs, Vijay V. Barve, Laura Brenskelle, Daijiang Li, Brian J. Stucky, Narayani V. Barve, Maggie M. Hantak, Bryan S. Mclean, Daniel J. Paluh, Jessica A. Oswald, Michael W. Belitz, Ryan A. Folk, Robert P. Guralnick
Methods For Broad-Scale Plant Phenology Assessments Using Citizen Scientists’ Photographs, Vijay V. Barve, Laura Brenskelle, Daijiang Li, Brian J. Stucky, Narayani V. Barve, Maggie M. Hantak, Bryan S. Mclean, Daniel J. Paluh, Jessica A. Oswald, Michael W. Belitz, Ryan A. Folk, Robert P. Guralnick
Faculty Publications
© 2020 Barve et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America Premise: Citizen science platforms for sharing photographed digital vouchers, such as iNaturalist, are a promising source of phenology data, but methods and best practices for use have not been developed. Here we introduce methods using Yucca flowering phenology as a case study, because drivers of Yucca phenology are not well understood despite the need to synchronize flowering with obligate pollinators. There is also evidence of recent anomalous winter flowering events, but with unknown spatiotemporal extents. Methods: We …
Impaired Purine Homeostasis Plays A Primary Role In Trimethoprim-Mediated Induction Of Virulence Genes In Burkholderia Thailandensis, Sudarshan S. Thapa, Anne Grove
Impaired Purine Homeostasis Plays A Primary Role In Trimethoprim-Mediated Induction Of Virulence Genes In Burkholderia Thailandensis, Sudarshan S. Thapa, Anne Grove
Faculty Publications
© 2020 John Wiley & Sons Ltd One of the most commonly prescribed antibiotics against Burkholderia infections is co-trimoxazole, a cocktail of trimethoprim and sulfamethoxazole. Trimethoprim elicits an upregulation of the mal gene cluster, which encodes proteins involved in synthesis of the cytotoxic polyketide malleilactone; trimethoprim does so by increasing expression of the malR gene, which encodes the activator MalR. We report that B. thailandensis grown on trimethoprim exhibited increased virulence against Caenorhabditis elegans. This enhanced virulence correlated with an increase in expression of the mal gene cluster. Notably, inhibition of xanthine dehydrogenase by addition of allopurinol led to similar …
The Extended Specimen Network: A Strategy To Enhance Us Biodiversity Collections, Promote Research And Education, James Lendemer, Barbara Thiers, Anna K. Monfils, Jennifer Zaspel, Elizabeth R. Ellwood, Andrew Bentley, Katherine Levan, John Bates, David Jennings, Dori Contreras, Laura Lagomarsino, Paula Mabee, Linda S. Ford, Robert Guralnick, Robert E. Gropp, Marcy Revelez, Neil Cobb, Katja Seltmann, M. Catherine Aime
The Extended Specimen Network: A Strategy To Enhance Us Biodiversity Collections, Promote Research And Education, James Lendemer, Barbara Thiers, Anna K. Monfils, Jennifer Zaspel, Elizabeth R. Ellwood, Andrew Bentley, Katherine Levan, John Bates, David Jennings, Dori Contreras, Laura Lagomarsino, Paula Mabee, Linda S. Ford, Robert Guralnick, Robert E. Gropp, Marcy Revelez, Neil Cobb, Katja Seltmann, M. Catherine Aime
Faculty Publications
No abstract provided.
The Worst Passions Of Human Nature: White Supremacy In The Civil War North, Andrew Turner
The Worst Passions Of Human Nature: White Supremacy In The Civil War North, Andrew Turner
Civil War Book Review
The New York City riots of July 1863 exhibited the potential of the violent tension that ran in a steady stream throughout the Northern states during the middle of the Civil War. As white New Yorkers rioted to oppose the Enrollment Act of 1863 imposed by the United States government, they quickly turned their ire toward African Americans, with deeply seated white supremacy and racially motivated fear prompting their deadly reprisals against innocent black New Yorkers. Sadly, as illustrated by Paul Escott’s needed new book, The Worst Passions of Human Nature: White Supremacy in the Civil War North, …
Informe Tecnico De Los Trabajos De Campo Del Proyecto De Investigación Arqueológica Cerro San Isidro, Valle De Nepeña, Costa De Ancash -- Temporada 2019, David Chicoine, Jeisen Navarro
Informe Tecnico De Los Trabajos De Campo Del Proyecto De Investigación Arqueológica Cerro San Isidro, Valle De Nepeña, Costa De Ancash -- Temporada 2019, David Chicoine, Jeisen Navarro
Faculty Publications
No abstract provided.
Phlebotomines (Diptera: Psychodidae) From A Urban Park Of Belém, Pará State, Northern Brazil And Potential Implications In The Transmission Of American Cutaneous Leishmaniasis, Yetsenia D.V. Sánchez Uzcátegui, Thiago Vasconcelos Dos Santos, Fernando T. Silveira, Patricia K.S. Ramos, Eduardo José M. Dos Santos, Marinete M. Póvoa, Lane Foil
Phlebotomines (Diptera: Psychodidae) From A Urban Park Of Belém, Pará State, Northern Brazil And Potential Implications In The Transmission Of American Cutaneous Leishmaniasis, Yetsenia D.V. Sánchez Uzcátegui, Thiago Vasconcelos Dos Santos, Fernando T. Silveira, Patricia K.S. Ramos, Eduardo José M. Dos Santos, Marinete M. Póvoa, Lane Foil
Faculty Publications
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America. In urban ecotourism parks, the life cycle of American cutaneous leishmaniasis (ACL) agents can remain established, where phlebotomines may comprise potential risks for visitors. The present study aimed to survey the phlebotomine fauna of a forest park 'Bosque Rodriques Alves-Jardim Botânico da Amazônia' (BRAJBA), in the urban area of Belém, Brazil. The park was monthly surveyed in 2018 using CDC light traps placed in ground and canopy strata. Leishmania spp. isolated from dissected females were characterized by polymerase chain reaction-restriction fragment length polymorphism …
Tropism, Pathology, And Transmission Of Equine Parvovirus-Hepatitis, Joy Ellen Tomlinson, Mason Jager, Alyssa Struzyna, Melissa Laverack, Lisa Ann Fortier, Edward Dubovi, Lane D. Foil, Peter D. Burbelo, Thomas J. Divers, Gerlinde R. Van De Walle
Tropism, Pathology, And Transmission Of Equine Parvovirus-Hepatitis, Joy Ellen Tomlinson, Mason Jager, Alyssa Struzyna, Melissa Laverack, Lisa Ann Fortier, Edward Dubovi, Lane D. Foil, Peter D. Burbelo, Thomas J. Divers, Gerlinde R. Van De Walle
Faculty Publications
© 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. Equine parvovirus-hepatitis (EqPV-H) has recently been associated with cases of Theiler's disease, a form of fulminant hepatic necrosis in horses. To assess whether EqPV-H is the cause of Theiler's disease, we first demonstrated hepatotropism by PCR on tissues from acutely infected horses. We then experimentally inoculated horses with EqPV-H and 8 of 10 horses developed hepatitis. One horse showed clinical signs of liver failure. The onset of hepatitis was temporally associated with seroconversion and …
Response Of Liver Metabolic Pathways To Ketogenic Diet And Exercise Are Not Additive, Tai Yu Huang, Felicia R. Goldsmith, Scott E. Fuller, Jacob Simon, Heidi M. Batdorf, Matthew C. Scott, Nabil M. Essajee, John M. Brown, David H. Burk, Christopher D. Morrison, Susan J. Burke, J. Jason Collier, Robert C. Noland
Response Of Liver Metabolic Pathways To Ketogenic Diet And Exercise Are Not Additive, Tai Yu Huang, Felicia R. Goldsmith, Scott E. Fuller, Jacob Simon, Heidi M. Batdorf, Matthew C. Scott, Nabil M. Essajee, John M. Brown, David H. Burk, Christopher D. Morrison, Susan J. Burke, J. Jason Collier, Robert C. Noland
Faculty Publications
© Lippincott Williams & Wilkins. Purpose Studies suggest ketogenic diets (KD) produce favorable outcomes (health and exercise performance); however, most rodent studies have used a low-protein KD, which does not reflect the normal- to high-protein KD used by humans. Liver has an important role in ketoadaptation due to its involvement in gluconeogenesis and ketogenesis. This study was designed to test the hypothesis that exercise training (ExTr) while consuming a normal-protein KD (NPKD) would induce additive/synergistic responses in liver metabolic pathways. Methods Lean, healthy male C57BL/6J mice were fed a low-fat control diet (15.9% kcal protein, 11.9% kcal fat, 72.2% kcal …
Chronicle Of A Tragedy: A Comparison Of Early Modern Iberian Literature To La Manada, Graceanne Lacombe
Chronicle Of A Tragedy: A Comparison Of Early Modern Iberian Literature To La Manada, Graceanne Lacombe
Honors Capstones
No abstract provided.
The Lonely Only: An Analysis Of The Theorizing Of United States Quota Culture Competiveness On The Modern-Day Black Woman, Russell Chamblee Wilder
The Lonely Only: An Analysis Of The Theorizing Of United States Quota Culture Competiveness On The Modern-Day Black Woman, Russell Chamblee Wilder
Honors Capstones
No abstract provided.
Louisiana Agriculture Winter, 2020, Lsu Agcenter
Louisiana Agriculture Winter, 2020, Lsu Agcenter
Louisiana Agriculture
No abstract provided.
Susceptibility Of Swine Cells And Domestic Pigs To Sars-Cov-2, David A. Meekins, Igor Morozov, Jessie D. Trujillo, Natasha N. Gaudreault, Dashzeveg Bold, Mariano Carossino, Bianca L. Artiaga, Sabarish V. Indran, Taeyong Kwon, Velmurugan Balaraman, Daniel W. Madden, Heinz Feldmann, Jamie Henningson, Wenjun Ma, Udeni B. R. Balasuriya, Juergen A. Richt
Susceptibility Of Swine Cells And Domestic Pigs To Sars-Cov-2, David A. Meekins, Igor Morozov, Jessie D. Trujillo, Natasha N. Gaudreault, Dashzeveg Bold, Mariano Carossino, Bianca L. Artiaga, Sabarish V. Indran, Taeyong Kwon, Velmurugan Balaraman, Daniel W. Madden, Heinz Feldmann, Jamie Henningson, Wenjun Ma, Udeni B. R. Balasuriya, Juergen A. Richt
Faculty Publications
No abstract provided.
Framework For A Community Health Observing System For The Gulf Of Mexico Region: Preparing For Future Disasters, P Sandifer, L Knapp, M Lichtveld, R Manley, P Sandifer, R Caffey, D Cochran, T Collier, K Ebi, L Engel, J Farrington, M Finucane, C Hale, D Halpern, E Harville, L Hart, Y Hswen, B Kirkpatrick, B Mcewen, G Morris, R Orbach, L Palinkas, M Partyka, D Porter, G Prather, T Rowles, G Scott, T Seeman, H Solo-Gabriele, E Svendsen, T Tincher, J Trtanj, A H. Walker, R Yehuda
Framework For A Community Health Observing System For The Gulf Of Mexico Region: Preparing For Future Disasters, P Sandifer, L Knapp, M Lichtveld, R Manley, P Sandifer, R Caffey, D Cochran, T Collier, K Ebi, L Engel, J Farrington, M Finucane, C Hale, D Halpern, E Harville, L Hart, Y Hswen, B Kirkpatrick, B Mcewen, G Morris, R Orbach, L Palinkas, M Partyka, D Porter, G Prather, T Rowles, G Scott, T Seeman, H Solo-Gabriele, E Svendsen, T Tincher, J Trtanj, A H. Walker, R Yehuda
Faculty Publications
The Gulf of Mexico (GoM) region is prone to disasters, including recurrent oil spills, hurricanes, floods, industrial accidents, harmful algal blooms, and the current COVID-19 pandemic. The GoM and other regions of the U.S. lack sufficient baseline health information to identify, attribute, mitigate, and facilitate prevention of major health effects of disasters. Developing capacity to assess adverse human health consequences of future disasters requires establishment of a comprehensive, sustained community health observing system, similar to the extensive and well-established environmental observing systems. We propose a system that combines six levels of health data domains, beginning with three existing, national surveys …
Who’S Afraid Of Ebola? Epidemic Fires And Locative Fears In The Information Age, W. Shrum, J. Aggrey, A. Campos, J. Pamplona Da Costa, J. Joseph, P. Kreimer, R. Kroeger, L. Medina, P. Miller, A. Palackal, A. Pandal De La Peza, A. Traore
Who’S Afraid Of Ebola? Epidemic Fires And Locative Fears In The Information Age, W. Shrum, J. Aggrey, A. Campos, J. Pamplona Da Costa, J. Joseph, P. Kreimer, R. Kroeger, L. Medina, P. Miller, A. Palackal, A. Pandal De La Peza, A. Traore
Faculty Publications
Epidemics have traditionally been viewed as the widespread occurrence of infectious disease within a community, or a sudden increase above what is typical. But modern epidemics are both more and less than the diffusion of viral entities. We argue that epidemics are 'fire objects', using a term coined by Law and Singleton: They generate locative fears through encounters that focus attention on entities that are unknown or imprecisely known, transforming spaces and humans into indeterminate dangers, alternating appearance and absence. The Ebola epidemic of 2014 had more complex impacts than the number of infections would suggest. We employ multi-sited qualitative …
Artificial Intelligence And Covid-19: Deep Learning Approaches For Diagnosis And Treatment, M. B. Jamshidi, A. Lalbakhsh, J. Talla, Z. Peroutka, F. Hadjilooei, P Lalbakhsh, M. Jamshidi, L. La Spada, M. Mirmozafari, M. Dehghani, A. Sabet, Sa. Roshani, So. Roshani, N. Bayat-Makou, B. Mohamadzade, Z. Malek, A. Jamshidi, S. Kiani, H. Hashemi-Dezaki, W. Mohyuddin
Artificial Intelligence And Covid-19: Deep Learning Approaches For Diagnosis And Treatment, M. B. Jamshidi, A. Lalbakhsh, J. Talla, Z. Peroutka, F. Hadjilooei, P Lalbakhsh, M. Jamshidi, L. La Spada, M. Mirmozafari, M. Dehghani, A. Sabet, Sa. Roshani, So. Roshani, N. Bayat-Makou, B. Mohamadzade, Z. Malek, A. Jamshidi, S. Kiani, H. Hashemi-Dezaki, W. Mohyuddin
Faculty Publications
COVID-19 outbreak has put the whole world in an unprecedented difficult situation bringing life around the world to a frightening halt and claiming thousands of lives. Due to COVID-19 & x2019;s spread in 212 countries and territories and increasing numbers of infected cases and death tolls mounting to 5,212,172 and 334,915 (as of May 22 2020), it remains a real threat to the public health system. This paper renders a response to combat the virus through Artificial Intelligence (AI). Some Deep Learning (DL) methods have been illustrated to reach this goal, including Generative Adversarial Networks (GANs), Extreme Learning Machine (ELM), …
Sars-Cov-2 Infection, Disease And Transmission In Domestic Cats, N N. Gaudreault, J D. Trujillo, M Carossino, D A. Meekins, N N. Gaudreault, D W. Madden, S V. Indran, D Bold, V Balaraman, T Kwon, B L. Artiaga, K Cool, A Garcia-Sastre, W J. Ma, W C. Wilson, J Henningson, U Balasuriya, J A. Richt
Sars-Cov-2 Infection, Disease And Transmission In Domestic Cats, N N. Gaudreault, J D. Trujillo, M Carossino, D A. Meekins, N N. Gaudreault, D W. Madden, S V. Indran, D Bold, V Balaraman, T Kwon, B L. Artiaga, K Cool, A Garcia-Sastre, W J. Ma, W C. Wilson, J Henningson, U Balasuriya, J A. Richt
Faculty Publications
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19) and responsible for the current pandemic. Recent SARS-CoV-2 susceptibility studies in cats show that the virus can replicate in these companion animals and transmit to other cats. Here, we present an in-depth study of SARS-CoV-2 infection, disease and transmission in domestic cats. Cats were challenged with SARS-CoV-2 via intranasal and oral routes. One day post challenge (DPC), two sentinel cats were introduced. Animals were monitored for clinical signs, clinicopathological abnormalities and viral shedding. Postmortem examinations were performed at 4, 7 and 21 DPC. Viral RNA …
Small Molecule Inhibitors Of The Bfrb-Bfd Interaction Decrease Pseudomonas Aeruginosa Fitness And Potentiate Fluoroquinolone Activity, Achala N.D.Punchi Hewage, Huili Yao, Baskar Nammalwar, Krishna Kumar Gnanasekaran, Scott Lovell, Richard A. Bunce, Kate Eshelman, Sahishna M. Phaniraj, Molly M. Lee, Blake R. Peterson, Kevin P. Battaile, Allen B. Reitz, Mario Rivera
Small Molecule Inhibitors Of The Bfrb-Bfd Interaction Decrease Pseudomonas Aeruginosa Fitness And Potentiate Fluoroquinolone Activity, Achala N.D.Punchi Hewage, Huili Yao, Baskar Nammalwar, Krishna Kumar Gnanasekaran, Scott Lovell, Richard A. Bunce, Kate Eshelman, Sahishna M. Phaniraj, Molly M. Lee, Blake R. Peterson, Kevin P. Battaile, Allen B. Reitz, Mario Rivera
Faculty Publications
© 2019 American Chemical Society. All rights reserved. The iron storage protein bacterioferritin (BfrB) is central to bacterial iron homeostasis. The mobilization of iron from BfrB, which requires binding by a cognate ferredoxin (Bfd), is essential to the regulation of cytosolic iron levels in P. aeruginosa. This paper describes the structure-guided development of small molecule inhibitors of the BfrB-Bfd protein-protein interaction. The process was initiated by screening a fragment library and followed by obtaining the structure of a fragment hit bound to BfrB. The structural insights were used to develop a series of 4-(benzylamino)- A nd 4-((3-phenylpropyl)amino)-isoindoline-1,3-dione analogs that selectively …
Female Reproductive State Is Associated With Changes In Distinct Arginine Vasotocin Cell Types In The Preoptic Area Of Astatotilapia Burtoni, Julie M. Butler, Chase M. Anselmo, Karen P. Maruska
Female Reproductive State Is Associated With Changes In Distinct Arginine Vasotocin Cell Types In The Preoptic Area Of Astatotilapia Burtoni, Julie M. Butler, Chase M. Anselmo, Karen P. Maruska
Faculty Publications
© 2020 Wiley Periodicals LLC Nonapeptides play a crucial role in mediating reproduction, aggression, and parental care across taxa. In fishes, arginine vasotocin (AVT) expression is related to social and/or reproductive status in most male fishes studied to date, and is linked to territorial defense, paternal care, and courtship. Despite a plethora of studies examining AVT in male fishes, relatively little is known about how AVT expression varies with female reproductive state or its role in female social behaviors. We used multiple methods for examining the AVT system in female African cichlid fish Astatotilapia burtoni, including immunohistochemistry for AVT, in …
Halobacterium Bonnevillei Sp. Nov., Halobaculum Saliterrae Sp. Nov. And Halovenus Carboxidivorans Sp. Nov., Three Novel Carbon Monoxide-Oxidizing Halobacteria From Saline Crusts And Soils, Marisa R. Myers, G. M. King
Halobacterium Bonnevillei Sp. Nov., Halobaculum Saliterrae Sp. Nov. And Halovenus Carboxidivorans Sp. Nov., Three Novel Carbon Monoxide-Oxidizing Halobacteria From Saline Crusts And Soils, Marisa R. Myers, G. M. King
Faculty Publications
© 2020 The Authors. Three novel carbon monoxide-oxidizing Halobacteria were isolated from Bonneville Salt Flats (Utah, USA) salt crusts and nearby saline soils. Phylogenetic analysis of 16S rRNA gene sequences revealed that strains PCN9T, WSA2T and WSH3T belong to the genera Halobacterium, Halobaculum and Halovenus, respectively. Strains PCN9T, WSA2T and WSH3T grew optimally at 40 °C (PCN9T) or 50 °C (WSA2T, WSH3T). NaCl optima were 3 M (PCN9T, WSA2T) or 4 M NaCl (WSH3T). Carbon monoxide was oxidized by all isolates, each of which contained a molybdenum-dependent CO dehydrogenase. G+C contents for the three respective isolates were 66.75, 67.62, and …
Speciation-By-Depth On Coral Reefs: Sympatric Divergence With Gene Flow Or Cryptic Transient Isolation?, Carlos Prada, Michael E. Hellberg
Speciation-By-Depth On Coral Reefs: Sympatric Divergence With Gene Flow Or Cryptic Transient Isolation?, Carlos Prada, Michael E. Hellberg
Faculty Publications
© 2020 The Authors. Journal of Evolutionary Biology published by John Wiley & Sons Ltd on behalf of European Society for Evolutionary Biology The distributions of many sister species in the sea overlap geographically but are partitioned along depth gradients. The genetic changes leading to depth segregation may evolve in geographic isolation as a prerequisite to coexistence or may emerge during primary divergence leading to new species. These alternatives can now be distinguished via the power endowed by the thousands of scorable loci provided by second-generation sequence data. Here, we revisit the case of two depth-segregated, genetically isolated ecotypes of …
The Avr4 Effector Is Involved In Cercosporin Biosynthesis And Likely Affects The Virulence Of Cercospora Cf. Flagellaris On Soybean, Josielle Santos Rezende, Marija Zivanovic, Maria Izabel Costa De Novaes, Zhi Yuan Chen
The Avr4 Effector Is Involved In Cercosporin Biosynthesis And Likely Affects The Virulence Of Cercospora Cf. Flagellaris On Soybean, Josielle Santos Rezende, Marija Zivanovic, Maria Izabel Costa De Novaes, Zhi Yuan Chen
Faculty Publications
One of the most devastating fungal diseases of soybean in the southern USA is Cercospora leaf blight (CLB), which is caused mainly by Cercospora cf. flagellaris. Recent studies found that the fungal effector AVR4, originally identified in Cladosporium fulvum as a chitin-binding protein, is highly conserved among other Cercospora species. We wanted to determine whether it is present in C. cf. flagellaris and, if so, whether it plays a role in the pathogen infection of soybean. We cloned the Avr4 gene and created C. cf. flagellaris ∆avr4 mutants, which produced little cercosporin and significantly reduced expression of cercosporin biosynthesis genes. …