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Site-Specific Deoxyribonucleic Acid Modifications For Use With Chemical Cages, John P. Casey Jr May 2009

Site-Specific Deoxyribonucleic Acid Modifications For Use With Chemical Cages, John P. Casey Jr

Honors Capstones

No abstract provided.


Louisiana Agriculture Winter, 2009 Jan 2009

Louisiana Agriculture Winter, 2009

Louisiana Agriculture

No abstract provided.


Preparation Of The Chinese Sweet Leaf Tea Extract And Its Anti-Obesity Effect In Rodents, Gar Yee Koh Jan 2009

Preparation Of The Chinese Sweet Leaf Tea Extract And Its Anti-Obesity Effect In Rodents, Gar Yee Koh

LSU Master's Theses

The aqueous leaf extract of Rubus suavissimus (Chinese sweet leaf tea) was active in inhibiting angiogenesis, which could be used to suppress adipogenesis and cause weight loss. Following this revelation and approach, the anti-obesity effect was thoroughly investigated in normal and obese rat models. The investigations began with the preparation of quality-controlled extract. First, the crude extraction methods were examined leading to the characterization of the chemical composition. Second, the impure components were removed by alcohol precipitation and column chromatography, resulting in a standardized sweet leaf tea extract (RUS). Third, three bioavailable compounds identified from the urine analyses were re-constituted …


Development And Application Of Dissociable Antibody Microarray (Dama) Staining Technique, Guanyuan Fu Jan 2009

Development And Application Of Dissociable Antibody Microarray (Dama) Staining Technique, Guanyuan Fu

LSU Doctoral Dissertations

Dissociable Antibody Microarray (DAMA) staining is a novel technique that integrates protein microarrays with conventional immunostaining techniques. It can simultaneously determine the expression and subcellular localizations (SCLs) of hundreds of proteins in cultured cells. I optimized this technology for protein expression and SCL profiling, and generated expression profile data analysis program DAMAPEP, molecular image database management program ChipView and automatic SCL assignment program DAMASCL. We demonstrated the application of this technique in the identification of potential biomarkers for breast cancer. We compared the expression profiles of 312 proteins among ten breast cell lines and identified 10 differentially expressed proteins. Among …


Bottom Boundary Layer Physics And Sediment Transport Along A Transgressive Sand Body, Ship Shoal, South-Central Louisiana: Implications For Fluvial Sediments And Winter Storms, Daijiro Kobashi Jan 2009

Bottom Boundary Layer Physics And Sediment Transport Along A Transgressive Sand Body, Ship Shoal, South-Central Louisiana: Implications For Fluvial Sediments And Winter Storms, Daijiro Kobashi

LSU Doctoral Dissertations

Ship Shoal, a shore-parallel sand body, was recently recognized as having a unique physical and biological environment and also as a potential sand resource for coastal restoration in coastal Louisiana. Little is known regarding such dynamics, in concert with fluvial sediments and winter storms, influenced in unique ecosystems, and likely in future potential sand mining. This dissertation addresses such the morphodynamics and sedimentary processes and their implications for the mining from the shoal using field measurements and numerical modeling studies. During the winter-spring season, fluvial sediment plumes shifted from the prevailing west to southeast during the post-frontal phases, resulted in …


Numerical Simulation Of Local Temperature Distortions During Ice Nucleation Of Cells In Suspension, D. Kandra, R. V. Devireddy Nov 2008

Numerical Simulation Of Local Temperature Distortions During Ice Nucleation Of Cells In Suspension, D. Kandra, R. V. Devireddy

Faculty Publications

Knowledge of intercellular ice formation in cells embedded in an extra-cellular suspension is essential for effective design of freezing protocols. The presence of cell membrane causes super-cooling of the intra-cellular region, which nucleates at much lower temperatures than the surrounding extra-cellular space and is accompanied by the exothermic release of the latent heat. This is a dynamic process and causes thermal distortions in and around the cell where nucleation occurs. In the present study, an attempt has been made to numerically determine the magnitude of thermal distortion (ΔT) and the time (dt) it takes for this distortion to damp out …


Carbon, Nitrogen, Sodium And Phosphorous Concentrations And Ratios In Spartina Alterniflora (Loiser) From Sites Of Various Freshwater And Nutrient Inputs In Coastal Louisiana Wetlands, Margaret Frances Williamson May 2008

Carbon, Nitrogen, Sodium And Phosphorous Concentrations And Ratios In Spartina Alterniflora (Loiser) From Sites Of Various Freshwater And Nutrient Inputs In Coastal Louisiana Wetlands, Margaret Frances Williamson

Honors Capstones

No abstract provided.


Syntheses And Evaluation Of Phthalocyanine Derivatives For Applications In Photodynamic And Boron Neutron Capture Therapies For Cancer, Hairong Li Jan 2008

Syntheses And Evaluation Of Phthalocyanine Derivatives For Applications In Photodynamic And Boron Neutron Capture Therapies For Cancer, Hairong Li

LSU Doctoral Dissertations

Chapter I introduced the background of phthalocyanines including its physical properties and various synthetic methods of free-base and metallo-phthalocyanines. The background of PDT and BNCT was introduced and the objective of ideal sensitizers was described. Chapter II described the synthesis and characterization of a series of pyridiloxy-substituted zinc phthalocyanines with different length of alkyl groups. Pyridiloxy-substituted silica phthalocyanines bearing different associated axial ligands, were alkylated with methyl or PEG chains. Biological study was performed on these cationic pyridiloxy-substituted zinc phthalocyanines. Chapter III introduced the synthesis and photophysical properties of A3B-type zinc phthalocyanines conjugated with one or two cobalta-carborane cages. They …


The Chlamydomonas Genome Reveals The Evolution Of Key Animal And Plant Functions, Sabeeha S. Merchant, Simon E. Prochnik, Olivier Vallon, Elizabeth H. Harris, Steven J. Karpowicz, George B. Witman, Astrid Terry, Asaf Salamov, Lillian K. Fritz-Laylin, Laurence Maréchal-Drouard, Wallace F. Marshall, Liang Hu Qu, David R. Nelson, Anton A. Sanderfoot, Martin H. Spalding, Vladimir V. Kapitonov, Qinghu Ren, Patrick Ferris, Erika Lindquist, Harris Shapiro, Susan M. Lucas, Jane Grimwood, Jeremy Schmutz, Igor V. Grigoriev, Daniel S. Rokhsar, Arthur R. Grossman, Pierre Cardol, Heriberto Cerutti, Guillaume Chanfreau, Chun Long Chen, Valérie Cognat, Martin T. Croft, Rachel Dent Oct 2007

The Chlamydomonas Genome Reveals The Evolution Of Key Animal And Plant Functions, Sabeeha S. Merchant, Simon E. Prochnik, Olivier Vallon, Elizabeth H. Harris, Steven J. Karpowicz, George B. Witman, Astrid Terry, Asaf Salamov, Lillian K. Fritz-Laylin, Laurence Maréchal-Drouard, Wallace F. Marshall, Liang Hu Qu, David R. Nelson, Anton A. Sanderfoot, Martin H. Spalding, Vladimir V. Kapitonov, Qinghu Ren, Patrick Ferris, Erika Lindquist, Harris Shapiro, Susan M. Lucas, Jane Grimwood, Jeremy Schmutz, Igor V. Grigoriev, Daniel S. Rokhsar, Arthur R. Grossman, Pierre Cardol, Heriberto Cerutti, Guillaume Chanfreau, Chun Long Chen, Valérie Cognat, Martin T. Croft, Rachel Dent

Faculty Publications

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ∼120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, …


The Benefits Of Bathing Buds: Water Calyces Protect Flowers From A Microlepidopteran Herbivore, Jane E. Carlson, Kyle E. Harms Aug 2007

The Benefits Of Bathing Buds: Water Calyces Protect Flowers From A Microlepidopteran Herbivore, Jane E. Carlson, Kyle E. Harms

Faculty Publications

Protective floral structures may evolve in response to the negative effects of floral herbivores. For example, water calyces - liquid-filled, cup-like structures resulting from the fusion of sepals - may reduce floral herbivory by submerging buds during their development. Our observations of a water-calyx plant, Chrysothemis friedrichsthaliana (Gesneriaceae), revealed that buds were frequently attacked by ovipositing moths (Alucitidae), whose larvae consumed anthers and stigmas before corollas opened. Almost 25% of per-plant flower production was destroyed by alucitid larvae over two seasons, far exceeding the losses to all other floral herbivores combined. Experimental manipulation of water levels in calyces showed that …


Deficits In Social Skills And Feeding Behaviors Associated With Adults Diagnosed With Autistic Disorder Living In An Institutionalized Setting, Cindy Terlonge Graham Jan 2007

Deficits In Social Skills And Feeding Behaviors Associated With Adults Diagnosed With Autistic Disorder Living In An Institutionalized Setting, Cindy Terlonge Graham

LSU Master's Theses

Autistic disorder, a syndrome beginning in infancy and persisting into adulthood, has captured the attention of researchers and clinicians alike. Although this disorder has been studied since the mid-1940s, there is a lack of literature addressing certain adaptive skills in adults with autism. The aim of this study was to examine the differences in social and feeding skills between individuals with and without autistic disorder. Given the importance of acquiring these skills to facilitate proper adjustment and decrease potential health risks, better understanding of these behaviors in persons with autism is warranted. Participants comprised three separate groups: an autism group, …


Juvenile Crawfish (Procambarus Clarkii) Lc50 Mortality From South Louisiana Crude, Peanut And Mineral Oil, Okey Umejuru Jan 2007

Juvenile Crawfish (Procambarus Clarkii) Lc50 Mortality From South Louisiana Crude, Peanut And Mineral Oil, Okey Umejuru

LSU Master's Theses

Nearly every continent has species of crawfish that inhabit wetlands or are near coastal areas where petroleum and other oils are produced, transported, and accidentally released. Most crawfish cannot tolerate polluted water. Thus, they could serve as biomonitors of fossil fuel, biofuel, and other oil releases and assess potential negative impacts such oils have on wetland and coastal ecosystems. The objectives of this study were to estimate the acute toxicity of three oils to juvenile crawfish and determine if toxicity was due to poisoning or interference with oxygen transfer across the gills. Juvenile crawfish (Procambarus clarkii) were tested for acute …


Fabrication Of Perforated Polymer Membranes Using Imprinting Technology, Anish Roychowdhury Jan 2007

Fabrication Of Perforated Polymer Membranes Using Imprinting Technology, Anish Roychowdhury

LSU Master's Theses

The goal of the present study was to fabricate perforated membrane structures in polymers with the pore diameter ranging from 10 ìm down to the sub ìm scale. The perforated membrane structure plays an important role in many biological systems. Thus the ability to reproduce such architecture will help with the study of biological systems by mimicking biological cell membrane-like structures and open new vistas in the study of transport behavior in cell biology and the separation of biological vesicles. Such structures also have potential uses as components in polymer optics and modular micro/nanofluidic devices. Use of polymer substrates is …


Synthesis And Properties Of Isoporphyrins And Related Derivatives For Application In Photodynamic Therapy, Sandra Celinah Mwakwari Jan 2007

Synthesis And Properties Of Isoporphyrins And Related Derivatives For Application In Photodynamic Therapy, Sandra Celinah Mwakwari

LSU Doctoral Dissertations

Isoporphyrins are tautomers of porphyrins with an interrupted macrocyclic conjugation owing to the presence of a sp3 hybridized meso-carbon. Due to their absorption at long wavelengths (~800nm), isoporphyrins are potential candidates as photosensitizers in photodynamic therapy (PDT), a ternary modality of cancer treatment. They are also of biological interest due to their unique redox properties among porphyrin derivatives and could be a new class of near-IR dyes. Isoporphyrins are known to be unstable with respect to transformation back to the fully conjugated porphyrin chromophore except when the meso-carbon is geminally substituted and for this reason a detailed study of this …


Floral Traits, Pollinator Behavior, And Plant Reproduction: Tests Of Natural And Sexual Selection In The Hummingbird-Pollinated Herb Chrysothemis Friedrichsthaliana, Jane Elizabeth Carlson Jan 2007

Floral Traits, Pollinator Behavior, And Plant Reproduction: Tests Of Natural And Sexual Selection In The Hummingbird-Pollinated Herb Chrysothemis Friedrichsthaliana, Jane Elizabeth Carlson

LSU Doctoral Dissertations

Pollinators may promote floral evolution by selectively visiting preferred floral phenotypes. Herbivores may also influence the evolution of floral traits, by reducing the fitness of poorly-defended plants, such as those without protective sepals. In this dissertation, I investigate the reproductive and evolutionary consequences of floral visitors for two floral traits of the Neotropical herb Chrysothemis friedrichsthaliana (Gesneriaceae). I focus on the trait male-biased nectar production, which results from coincident temporal shifts in nectar production rates and sexual function within flowers. First, I review theory relevant to male- and female-biased nectar production, and I generate hypotheses to explain its evolution in …


The Spectral And Photophysical Characterization Of Water Solubilizing Metal Phthalocyanines For Highly Sensitive Detection In Biological Studies, Vera Tamisha Verdree Jan 2007

The Spectral And Photophysical Characterization Of Water Solubilizing Metal Phthalocyanines For Highly Sensitive Detection In Biological Studies, Vera Tamisha Verdree

LSU Doctoral Dissertations

Efforts to improve the technology for DNA analyses stimulated the work presented here to develop a series of fluorescent probes that would aid in the increased multiplexing capabilities allowing the ability to detect multiple targets in a single measurement. Typically, spectral discrimination has been used to identify each DNA fragment in sequencing or diagnostic applications by means of unique emission properties from several labeling dyes. This has been accomplished with labels that fluoresce in the visible region. The limitations with these dyes is the broad emission profile making discrimination difficult, high intrinsic biological fluorescence and structural differences of the dyes …


Syntheseses [Sic] And Evaluation Of Porphyrin Derivatives For Applications In Medicine And In Material Science, Erhong Hao Jan 2007

Syntheseses [Sic] And Evaluation Of Porphyrin Derivatives For Applications In Medicine And In Material Science, Erhong Hao

LSU Doctoral Dissertations

Chapter 1 presents a concise introduction to porphyrins, especially on the synthesis of meso-tetraphenylporphyrins, which were synthesized during the course of my research and were used for further purposes in the following chapters. Chapter 2 describes the synthesis of a series of cobaltacarborane-porphyrin conjugates for the boron neutron capture therapy (BNCT) of tumors. Using an efficient and high yield ring-opening reaction, N-substituted porphyrins were also synthesized. The spectroscopic and structural properties of these compounds are presented. These high percentage boron and amphiphilic compounds are promising boron delivery agents for BNCT (prelimary cellular studies using some of these compounds have been …


Synthesis And Functionalizations Of Tetrapyrrole Derivatives, Lijuan Jiao Jan 2007

Synthesis And Functionalizations Of Tetrapyrrole Derivatives, Lijuan Jiao

LSU Doctoral Dissertations

Chapter 1 of this Dissertation presents a brief overview of the history of tetrapyrrole derivatives and of their fundamental properties. Overviews of porphyrins, benzoporphyrins, chlorins and porphycenes are presented. Work presented in Chapter 2 through Chapter 4 mainly focuses on the syntheses and functionalization of chromophore-extended porphyrin derivatives. Several new synthetic routes for the syntheses and functionalizations of extended porphyrins either at the β-position or at the meso-position of porphyrin are developed. From these improved synthetic routes, the regio-selective syntheses of porphyrin derivatives are described. Chapter 2 mainly focuses on the syntheses of β,β'-fused methylenepropanoporphyrins and related porphyrin dimers. Chapter …


Adult Phyllophaga Ephilida Host Plant Feeding Preference, Aboubacar Diagne, Richard N. Story, Abner M. Hammond Sep 2006

Adult Phyllophaga Ephilida Host Plant Feeding Preference, Aboubacar Diagne, Richard N. Story, Abner M. Hammond

Faculty Publications

Limited biological information about Phyllophaga ephilida, a major sweet potato pest in Louisiana, is available. In 2001 and 2002, a study was conducted in the laboratory to investigate the feeding preference of adult Phyllophaga ephilida (Say) (Coleoptera: Scarabaeidae) for the foliage of eight woody plant species: water oak (Quercus nigra L.), live oak (Quercus virginiana Mill.), red maple (Acer rubrum L.), slash pine (Pinus caribaea Morelet), pecan (Carya illinoensis (Wangenh) K. Koch), sweetgum (Liquidambar styraciflua L.), southern magnolia (Magnolia grandiflora L.), and American elm (Ulmus americana L.). Beetles were placed in an arena with the eight host plants and allowed …


Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco (Nicotiana Tabacum L.) Leaf Disks: Evaluation Of The Co-Cultivation Conditions To Increase Beta-Glucuronidase Gene Activity, Sunjung Park Jan 2006

Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco (Nicotiana Tabacum L.) Leaf Disks: Evaluation Of The Co-Cultivation Conditions To Increase Beta-Glucuronidase Gene Activity, Sunjung Park

LSU Master's Theses

Agrobacterium tumefaciens-mediated transformation is generally used for genetic transformation of higher plants. Several experimental factors important for the increase of beta-glucuronidase (GUS) reporter gene activity were evaluated in this study using leaf disks of tobacco (Nicotiana tabacum L. cv. Xanthi). We found that co-cultivation temperature at 20C is the most critical factor to obtain the reproducible enhancement of GUS activity. pCAMBIA 1305.01 resulted in higher GUS activity than the other two pCAMBIA vectors 1301 and 1305.02. The highest GUS activity and transformation efficiency were achieved under the following experimental conditions: Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA1305.01 was grown overnight at …


Cellular Efflux Of Auxin Catalyzed By The Arabidopsis Mdr/Pgp Transporter Atpgp1, Markus Geisler, Joshua J. Blakeslee, Rodolphe Bouchard, Ok Ran Lee, Vincent Vincenzetti, Anindita Bandyopadhyay, Boosaree Titapiwatanakun, Wendy Ann Peer, Aurèlien Bailly, Elizabeth L. Richards, Karin F.K. Ejendal, Aaron P. Smith, Célia Baroux, Ueli Grossniklaus, Axel Müller, Christine A. Hrycyna, Robert Dudler, Angus S. Murphy, Enrico Martinoia Oct 2005

Cellular Efflux Of Auxin Catalyzed By The Arabidopsis Mdr/Pgp Transporter Atpgp1, Markus Geisler, Joshua J. Blakeslee, Rodolphe Bouchard, Ok Ran Lee, Vincent Vincenzetti, Anindita Bandyopadhyay, Boosaree Titapiwatanakun, Wendy Ann Peer, Aurèlien Bailly, Elizabeth L. Richards, Karin F.K. Ejendal, Aaron P. Smith, Célia Baroux, Ueli Grossniklaus, Axel Müller, Christine A. Hrycyna, Robert Dudler, Angus S. Murphy, Enrico Martinoia

Faculty Publications

Directional transport of the phytohormone auxin is required for the establishment and maintenance of plant polarity, but the underlying molecular mechanisms have not been fully elucidated. Plant homologs of human multiple drug resistance/P-glycoproteins (MDR/PGPs) have been implicated in auxin transport, as defects in MDR1 (AtPGP19) and AtPGP1 result in reductions of growth and auxin transport in Arabidopsis (atpgp1, atpgp19), maize (brachytic2) and sorghum (dwarf3). Here we examine the localization, activity, substrate specificity and inhibitor sensitivity of AtPGP1. AtPGP1 exhibits non-polar plasma membrane localization at the shoot and root apices, as well as polar localization above the root apex. Protoplasts from …


Yellow Fever In The Felicianas: The Epidemic Of 1878 And Its Effects Upon The Residents Of These Rural Parishes, Mary Jane Duke Russell Jan 2005

Yellow Fever In The Felicianas: The Epidemic Of 1878 And Its Effects Upon The Residents Of These Rural Parishes, Mary Jane Duke Russell

LSU Master's Theses

This research documents the spread of yellow fever across the rural Louisiana parishes of East Feliciana and West Feliciana in 1878 and examines the reactions and responses of the residents to medical, social and economic stresses produced by that epidemic. Descriptive details highlight the variability of individual ideas and mindsets at play against the backdrop of accepted paradigms, belief systems and current technology. In 1878 the Aëdes aegypti mosquito had not yet been identified as the vector of the arbovirus (arthropod borne virus) that causes yellow fever. A short history of yellow fever in the United States and a discussion …


Synthetic Development And Application Of Compounds With Biological Relevance, Jeffery Caleb Clark Jan 2005

Synthetic Development And Application Of Compounds With Biological Relevance, Jeffery Caleb Clark

LSU Doctoral Dissertations

Nature has provided a vast number of compounds that have medicinal application. The use of these compounds provided by nature however, is limited by the amount of a specific compound that nature provides. The synthesis of these molecules and their derivatives provides an even greater number of compounds for biological screening. Various pyrroles, the heterocyclic building blocks of porphyrins, can be prepared synthetically over several steps. The implementation of carboranes on pyrrole rings intrinsically provides substrates that can be converted into porphyrins bearing carborane substituents. The condensation of these carboranylpyrroles with carboranylaldehydes provides carboranylporphyrins bearing a higher order of carborane …


Seasonal Occurrence Of Phyllophaga Species And Biological Studies Of Phyllophage Ephilida (Say) On Sweet Potato, Ipomoea Batatas (L) Lam, In Louisiana, Aboubacar Diagne Jan 2004

Seasonal Occurrence Of Phyllophaga Species And Biological Studies Of Phyllophage Ephilida (Say) On Sweet Potato, Ipomoea Batatas (L) Lam, In Louisiana, Aboubacar Diagne

LSU Doctoral Dissertations

The biology of Phyllophaga ephilida (Say) was studied in Louisiana with both laboratory and field experiments from 1998 through 2003. The seasonal occurrence of common adult Phyllophaga species in sweet potato growing areas was surveyed with blacklight traps. P. ephilida and P. crinita (Burmeister) were the most common species at all locations. The attractiveness of seven methyl esters of L-isoleucine and L-valine blends to P. ephilida males was evaluated in sweet potato fields in 1999 and 2000. The 100% methyl ester of L-isoleucine was significantly more attractive than all other blend ratios that were tested. The life cycle of P. …


Evaluation Of Injury And Management Strategies For Stink Bugs (Heteroptera: Pentatomidae) On Cotton, Gossypium Hirsutum L., Melissa Marie Willrich Jan 2004

Evaluation Of Injury And Management Strategies For Stink Bugs (Heteroptera: Pentatomidae) On Cotton, Gossypium Hirsutum L., Melissa Marie Willrich

LSU Doctoral Dissertations

The effects of brown stink bug, Euschistus servus (Say), and southern green stink bug, Nezara viridula (L.), feeding on pre-flowering, flowering, and senescing cotton, Gossypium hirsutum L., plants were evaluated in field studies. Vegetative stage seedlings and flower buds (squares) were not significantly injured by adults or nymphs of either species in no-choice studies. Brown stink bug adults induced boll abscission, and reduced seedcotton yield and seed germination in bolls accumulating „T 350, „T 550, and 101 to „T 600 heat units beyond anthesis, respectively. In free-choice tests, boll preference was evaluated during each of the initial five weeks of …


Agronomic And Molecular Characterization Of Louisiana Native Spartina Alterniflora Accessions, Alicia Beatriz Ryan Jan 2003

Agronomic And Molecular Characterization Of Louisiana Native Spartina Alterniflora Accessions, Alicia Beatriz Ryan

LSU Doctoral Dissertations

Coastal erosion and wetland deterioration are serious and widespread problems affecting Louisiana’s coastal zone. The application of agronomic and molecular techniques for improving crop species is well documented. However, these have not been routinely applied to species of ecological and environmental value. Spartina alterniflora is used extensively for shoreline protection and tidal marsh restoration because of its aggressive spreading habit and tolerance to salinity. The progress of marsh revegetation projects is limited by the costs and labor associated with vegetative propagation of Spartina. Hence, a breeding program was initiated to develop improved smooth cordgrass accessions with superior seed producing ability …


Genome Analysis: Mutation Analysis Using Near Infrared Laser-Induced Fluorescence (Nir-Lif) And Single Molecule Detection In Microfluidic Devices, Musundi Ben Wabuyele Jan 2003

Genome Analysis: Mutation Analysis Using Near Infrared Laser-Induced Fluorescence (Nir-Lif) And Single Molecule Detection In Microfluidic Devices, Musundi Ben Wabuyele

LSU Doctoral Dissertations

A number of genotyping methods have been developed for mutation analysis, each of which has its own unique advantage. DNA amplification via polymerase chain reaction (PCR) provides an unlimited supply of material for subsequent genetic analysis even in case where only a single copy of the DNA molecule is present in the sample. Research presented in this dissertation first examines the efficiency of in vitro amplification of single copy DNA with subsequent sequencing analysis of PCR product. The PCR products (amplicons) were investigated for possible alteration, distortions or mutations due to the amplification process. The sequencing data for single copy …


Determination And Consideration Of Flight Potential In A Laboratory Population Of True Armyworm (Lepidoptera: Noctuidae), Lizhi Luo, S. J. Johnson, A. M. Hammond, J. D. Lopez, J. P. Geaghan, K. R. Beerwinkle, J. K. Westbrook Jan 2002

Determination And Consideration Of Flight Potential In A Laboratory Population Of True Armyworm (Lepidoptera: Noctuidae), Lizhi Luo, S. J. Johnson, A. M. Hammond, J. D. Lopez, J. P. Geaghan, K. R. Beerwinkle, J. K. Westbrook

Faculty Publications

Flight potential, a poorly understood phenomenon in the migratory true armyworm, Pseudaletia unipuncta (Haworth), was investigated in a laboratory population using tethered-flight techniques. True armyworm exhibited strong flight potential relative to other known migratory noctuids whose flight potentials were previously determined by similar techniques. Flight potential was generally low for 1-d-old moths and increased with age to day 5 of adult life. Flight speed and distance flown decreased for female moths from day 5 to day 10 as their reproductive system developed. The greatest average flight speed, total flight duration, and distance flown was exhibited by 5-d-old females and 10-d-old …


Reproductive Biology Of Smooth Cordgrass (Spartina Alterniflora), Xiaobing Fang Jan 2002

Reproductive Biology Of Smooth Cordgrass (Spartina Alterniflora), Xiaobing Fang

LSU Master's Theses

Smooth cordgrass (S. alterniflora) is a perennial grass that dominates the salt marsh in tidal wetlands along the Atlantic and Gulf Coast of North America and has been used for preventing soil erosion and restoring wetlands. Accessions collected from south Louisiana were studied to investigate flowering phenology, pollen viability, crossability, and seed production. S. alterniflora exhibited protogynous flowering where stigmas exserted 2 to 5 days from the floret prior to anthesis. Pollen shed primarily between 8:00 and 10:00 AM. Pollens were viable with average germination of 69% and stigma was receptive after exsertion. Pollen germinated in 15 minutes and pollen …


Artificial Peptides Containing Ca,A- Disubstituted Amino Acids: Synthesis, Conformational Studies, And Application As Β-Strand Mimics, Yanwen Fu Jan 2002

Artificial Peptides Containing Ca,A- Disubstituted Amino Acids: Synthesis, Conformational Studies, And Application As Β-Strand Mimics, Yanwen Fu

LSU Doctoral Dissertations

Short peptides containing Cα,α-dipropylglycine (Dpg) at alternating sequence positions were synthesized and examined for conformational behavior. Peptide assembly was performed using Fmoc-solid-phase chemistry where the coupling with PyAOP could be significantly enhanced at elevated temperature. Circular dichroism (CD) and NMR conformational studies revealed that incorporation of Dpg residues induced folded structures into peptides. It was observed that Dpg residues adopted helical conformation in a helix-promoting sequence. The resulting helical structure was comprised of consecutive β-turns whose structure was stabilized by salt bridge in aqueous solution. In this study, the preparation of sterically and polyfunctional Cα,α-disubstituted amino …