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The Significance Of Beta-72 Asparagine Methylation In C-Phycocyanin., Beth Ann Thomas
The Significance Of Beta-72 Asparagine Methylation In C-Phycocyanin., Beth Ann Thomas
LSU Historical Dissertations and Theses
A novel post-translationally modified asparagine, $\gamma$-N-methylasparagine (NMA), has been characterized at the $\beta$-72 site in many phycobiliproteins. The behavior of a NMA-containing peptide under Edman degradation conditions employed for sequence analysis was examined; NMA can be reproducibly identified in protein sequences by careful attention to repetitive yields and the presence of minor peaks. The effects of asparagine methylation on photosynthetic rates in the cyanobacterium Synechococcus PCC 7942 and two methylase-minus mutants was measured by steady-state oxygen evolution in whole cells. The methylase-minus mutants demonstrated lower rates of electron transfer through Photosystem II under conditions in which phycobilisomes were preferentially illuminated …
Differential Susceptibility Of Guanine Nucleotide-Binding Proteins To Pertussis Toxin-Catalyzed Adp-Ribosylation In Brain Membranes Of Two Congeneric Marine Fishes, T. F. Murray, J. F. Siebenaller
Differential Susceptibility Of Guanine Nucleotide-Binding Proteins To Pertussis Toxin-Catalyzed Adp-Ribosylation In Brain Membranes Of Two Congeneric Marine Fishes, T. F. Murray, J. F. Siebenaller
Faculty Publications
No abstract provided.
Structures Of And Allelic Diversity And Relationships Among The Major Outer Membrane Protein (Ompa) Genes Of The Four Chlamydial Species, B. Kaltenboeck, K. G. Kousoulas, J. Storz
Structures Of And Allelic Diversity And Relationships Among The Major Outer Membrane Protein (Ompa) Genes Of The Four Chlamydial Species, B. Kaltenboeck, K. G. Kousoulas, J. Storz
Faculty Publications
DNA sequences coding for 81% of the ompA gene from 24 chlamydial strains, representing all chlamydial species, were determined from DNA amplified by polymerase chain reactions. Chlamydial strains of serovars and strains with similar chromosomal restriction fragment length polymorphism had identical ompA DNA sequences. The ompA sequences were segregated into 23 different ompA alleles and aligned with each other, and phylogenetic relationships among them were inferred by neighbor-joining and maximum parsimony analyses. The neighbor-joining method produced a single phylogram which was rooted at the branch between two major clusters. One cluster included all Chlamydia trachomatis ompA alleles (trachoma group). The …
Glycine And Alanine Supplementation Of Culture Medium Enhances Development Of In Vitro Matured And Fertilized Cattle Embryos, K. Moore, K. R. Bondioli
Glycine And Alanine Supplementation Of Culture Medium Enhances Development Of In Vitro Matured And Fertilized Cattle Embryos, K. Moore, K. R. Bondioli
Faculty Publications
One-cell cattle embryos were prepared by in vitro oocyte maturation and fertilization (IVM/IVF) and cultured with or without oviductal cells. Embryos were evaluated after 7 days in culture to determine the percentage developing from the 1-cell stage to the morula or blastocyst stage. The combination of glycine (2 mM) and alanine (1 mM) with oviductal cells (experiment 1) improved embryo development over that in control culture (29 vs. 13%; p < 0.05). An optimum response was obtained with 10 mM glycine and 1 mM alanine in coculture (experiments 2 and 3). In experiment 4, the effects of glycine (0 or 10 mM), alanine (0 or 1 mM), and the presence or absence of oviductal cells were tested. In the absence of oviductal cells, the addition of glycine, alanine, or glycine and alanine combined improved embryonic development over that in control medium (45, 33, 42 vs. 24%, p < 0.001, p < 0.05, p < 0.001, respectively). However, the effect of the combination of glycine and alanine was not different from that of glycine alone when oviductal cells were absent (42 vs. 45%, p > 0.10). In the presence of oviductal cells, glycine or the combination of glycine and alanine improved embryonic development over that in the control medium with cells (47, 55 vs. 37%, …
Response Of Early Lactation Cows To Fat Supplementation In Diets With Different Nonstructural Carbohydrate Concentrations, James C. Coomer, Henry E. Amos, Cathy C. Williams, Jeffrey G. Wheeler
Response Of Early Lactation Cows To Fat Supplementation In Diets With Different Nonstructural Carbohydrate Concentrations, James C. Coomer, Henry E. Amos, Cathy C. Williams, Jeffrey G. Wheeler
Faculty Publications
Twenty-four high producing (36.7 kg/d, initially) Holstein cows in early lactation were used in a 63-d lactation trial to study the effects of low (28%), medium (31%). and high (37%) nonstructural carbohydrate concentrations in diets with added fat. Diets with isonitrogenous (17% CP) and fed as TMR with 41% sorghum silage. Nonstructural carbohydrate concentrations were altered by substitution of corn gluten feed and soybean hulls (50:50) for wheat and corn in the concentrate portion of the diet. Fat was added at 3% of DM in the form of yellow grease. Production of milk (38.5, 38.9, and 40.4 kg/d) and 3.5% …
Response Of Early Lactation Cows To Fat Supplementation In Diets With Different Nonstructural Carbohydrate Concentrations, James C. Coomer, Henry E. Amos, Cathy C. Williams, Jeffrey G. Wheeler
Response Of Early Lactation Cows To Fat Supplementation In Diets With Different Nonstructural Carbohydrate Concentrations, James C. Coomer, Henry E. Amos, Cathy C. Williams, Jeffrey G. Wheeler
Faculty Publications
Twenty-four high producing (36.7 kg/d, initially) Holstein cows in early lactation were used in a 63-d lactation trial to study the effects of low (28%), medium (31%). and high (37%) nonstructural carbohydrate concentrations in diets with added fat. Diets with isonitrogenous (17% CP) and fed as TMR with 41% sorghum silage. Nonstructural carbohydrate concentrations were altered by substitution of corn gluten feed and soybean hulls (50:50) for wheat and corn in the concentrate portion of the diet. Fat was added at 3% of DM in the form of yellow grease. Production of milk (38.5, 38.9, and 40.4 kg/d) and 3.5% …
Louisiana Agriculture Magazine, Winter 1993, Lsu Agricultural Experiment Station
Louisiana Agriculture Magazine, Winter 1993, Lsu Agricultural Experiment Station
Louisiana Agriculture
No abstract provided.
Methodological Content And Teaching Delivery Used By Vocational Teacher Education Faculty In University Council And Other Selected Higher Education Institutions., Robert Barry Blair
Methodological Content And Teaching Delivery Used By Vocational Teacher Education Faculty In University Council And Other Selected Higher Education Institutions., Robert Barry Blair
LSU Historical Dissertations and Theses
The purpose of this study was to identify the methodological content and teaching delivery used by vocational teacher education faculty in University Council for Vocational Education (UCVE) institutions and other selected higher education institutions (NON-UCVE). The population for this study was defined as: full-time vocational teacher education faculty in programs at Research I, Research II, and Doctorate Granting Colleges and Universities I where comprehensive vocational education programs were offered. Administrative leaders for each institution identified the vocational teacher education faculty which established the frame for the study. A stratified-random cluster sample of vocational teacher educators from UCVE institutions and NON-UCVE …
Investigations Into Methods For The Extraction And Detection Of Chlorinated Pesticides In Aquatic Animals., Heidi Marie Lott
Investigations Into Methods For The Extraction And Detection Of Chlorinated Pesticides In Aquatic Animals., Heidi Marie Lott
LSU Historical Dissertations and Theses
Methodologies for the extraction and analysis of chlorinated pesticides from aquatic species are reviewed and compared. New multiresidue isolation techniques using Matrix Solid Phase Dispersion (MSPD) are presented for the extraction and subsequent gas chromatographic, electron capture detector determination of 14 chlorinated pesticides ($\alpha$-BHC, $\beta$-BHC, lindane, heptachlor, aldrin, heptachlor epoxide, p,p$\sp\prime$-DDE, dieldrin, endrin, 4,4$\sp\prime$-DDD, endrin aldehyde, p,p$\sp\prime$-DDT, endosulfan sulfate, and methoxychlor) from crayfish (Procambarus clarkii) hepatopancreas, oysters (Crassostrea virginica) and fish muscle tissues. Pureed crayfish hepatopancreata or oysters or fish muscle fillets (0.5-g aliquots) were fortified with the 14 pesticides, plus $\delta$-BHC as an internal standard, before being blended with …
Immunologic And Pathologic Responses Of Macaca Mulatta Experimentally Infected With Simian Immunodeficiency Virus And Campylobacter Jejuni., Robert Wayne Kornegay
Immunologic And Pathologic Responses Of Macaca Mulatta Experimentally Infected With Simian Immunodeficiency Virus And Campylobacter Jejuni., Robert Wayne Kornegay
LSU Historical Dissertations and Theses
Five Macaca mulatta were infected with SIV/DELTA$\sb{\rm B670}.$ Approximately 70 days later, this group (SIV) and five age, sex, weight matched not SIV-infected (NEG) M. mulatta were inoculated orally with approximately $3\times10\sp{10}$ Campylobacter jejuni. Small and large bowel mucosal biopsies, bowel contents, sera, blood, and rectal swabs were collected before and after C. jejuni inoculation. Biopsy cryosections were stained with histologic stains and immunocytochemically for lymphocyte subsets, plasmacytes, macrophages, and B-cells. Coded stained sections were examined semiquantitatively or quantitatively using computerized image analysis. Circulating lymphocyte subsets were determined using FACS analysis. Campylobacter jejuni was recultured from 4/5 SIV and 4/5 …
Windows To Critical Thinking: Perspectives On Pedagogy In Henry James's "The Bostonians", Carson Mccullers's "The Heart Is A Lonely Hunter", And Frederick Douglass's "Narrative Of The Life Of Frederick Douglass, An American Slave"., James Thomas Mullane
LSU Historical Dissertations and Theses
Many times teachers restrict students to viewing literature through a single "preferred" window or from a rather small set of tiny windows, thus hindering their ability to think critically and make choices for themselves. It is my contention that our pedagogy should facilitate a "school of windows" in which students are offered many different vantage points from which to "see" literature--an environment which, instead of monologically conditioning them to accept content without criticism or question, dialogically allows them their own place and importance in active discourse. This study delineates the power that literature has to enable students to acquire these …
The Absent Father In Modern Drama., Paul Nagim Rosefeldt
The Absent Father In Modern Drama., Paul Nagim Rosefeldt
LSU Historical Dissertations and Theses
Theories of modern drama point out the powerful sense of loss and alienation that is woven into the very fabric of modern drama. This sense of loss is connected with the death of God and the destruction of absolute value systems. This study, however, takes the theory of loss one step further, tying the absence of God in modern drama to the absence of the father. The psychoanalytical theories of Sigmund Freud, Jacques Lacan, and Carl Jung view the father as a disembodied presence standing above and outside of culture. These psychological theories of the father can be connected to …
Daily Stress And Smoking., Kathryn Leigh Nathan
Daily Stress And Smoking., Kathryn Leigh Nathan
LSU Historical Dissertations and Theses
This study examined daily minor stress and cigarette smoking in adult habitual smokers. In this study, 55 subjects monitored daily stress, daily state anxiety, and daily cigarette intake for 21 consecutive days. Subjects also completed measures of trait anxiety, self-reported smoking motives, recent major life events, and social support. These variables, along with gender, were used to predict associations between daily cigarette intake and scores on daily stress and anxiety inventories. Results of within- and between-subjects time series correlational analyses showed significant associations between scores on measures of daily stress and daily cigarette intake. While subjects as individuals showed marked …
Nitrogen Composition And Feeding Value Of Annual Forages As Affected By Nitrogen Fertilization And Defoliation Frequency., Youbo Zhang
LSU Historical Dissertations and Theses
Initially, various solvents were tested for their efficacy in isolating soluble nitrogen (SN) from annual ryegrass and berseem clover. Plant nitrogen (PN) solubilities were similar in.5 M NaCl, autoclaved rumen fluid, borate phosphate buffer and Burroughs' solution. The mean PN solubilities across forages ranged from 12% for.1 M acetic acid to 45% for.2% NaOH. At 4$\sp\circ$C, 15% TCA precipitated the most soluble plant protein nitrogen (SPPN). Nitrogen (N) yield, concentrations of PN, SN, SPPN and soluble nonprotein nitrogen (SNPN) increased linearly as N fertilization (NF) increased from 0 to 335 kg N/ha. Decreasing defoliation interval (DI) from 6- to 2-wk …
Dual Diagnosis In Persons With Severe Or Profound Mental Retardation: A Reliability Study Of The Dash., Jay Anthony Sevin
Dual Diagnosis In Persons With Severe Or Profound Mental Retardation: A Reliability Study Of The Dash., Jay Anthony Sevin
LSU Historical Dissertations and Theses
The present study was designed to extend the literature on assessing psychopathology in persons with mental retardation. The primary goal of the study was to evaluate the psychometric properties of the Diagnostic Assessment for the Severely Handicapped (DASH), focusing on reliability issues. A secondary goal was to examine the prevalence of psychopathology and behavior problems in the severely handicapped. Subjects included 658 institutionalized adults with severe or profound mental retardation. Adequate reliability of DASH items and subscales, with a few notable exceptions, was obtained. Prevalence rates of various behavior disorders and symptoms of mental illness are presented. Practical implications of …
Structural Analysis And Methionine Ehancement Of The Bean Seed Storage Protein Phaseolin., John M. Dyer
Structural Analysis And Methionine Ehancement Of The Bean Seed Storage Protein Phaseolin., John M. Dyer
LSU Historical Dissertations and Theses
Common beans are widely utilized as a food source, yet are limited as a complete source of protein due to low levels of methionine, an essential amino acid for humans. A protein engineering strategy was developed to increase the methionine content of phaseolin, the primary seed storage protein in common beans. The engineering strategy consists of three major parts. In the first part, a set of biophysical probes was developed to characterize the stability of wild-type and modified phaseolin proteins. I used absorbance, fluorescence emission, circular dichroism, and fluorescence polarization anisotropy to monitor phaseolin denaturation induced by urea, guanidinium chloride, …
Depressive Symptoms In Prepubertal Children., Lesley Stabinsky Compton
Depressive Symptoms In Prepubertal Children., Lesley Stabinsky Compton
LSU Historical Dissertations and Theses
This study was designed: (1) to explore interinformant reliability between preadolescent students, their teachers, and their parents; (2) to investigate the use of standardized and non-standardized academic variables as predictors of depression; and (3) to assess differences in depression between black and white students. One hundred and twenty, fourth and fifth grade students and teachers participated. Sixty-eight parents also volunteered for this study. The Child Depression Inventory and the modified Bellevue Index of Depression were administered to students, teachers, and parents. Significant but moderate interinformant reliability was found between teachers and students and between teachers and parents on both assessment …
The Development Of An Inventory To Measure Bereavement., David Nicholas Hayes
The Development Of An Inventory To Measure Bereavement., David Nicholas Hayes
LSU Historical Dissertations and Theses
This study describes the development of a self-report inventory to measure bereavement. The inventory was called the Hayes Bereavement Inventory (HBI). Items for the inventory were provided by four grief therapists with at least three years of experience in bereavement counseling. Items were written in a format similar to the Beck Depression Inventory. Four hundred and forty adults bereaved in the past five years completed the bereavement inventory, three widely used self-report measures of depression, anxiety, and anger, and the Eysenck Personality Questionnaire. Respondents lost loved ones to death from a variety of causes which included medical diseases, car accidents, …
Psychosocial Co-Factors And Hiv Disease Status., Bradley Thomas Thomason
Psychosocial Co-Factors And Hiv Disease Status., Bradley Thomas Thomason
LSU Historical Dissertations and Theses
Concern over the devastating effects of the Human Immunodeficiency Virus (HIV) has been met with rigorous research efforts by allied health professions. Identifying variables, or co-factors, that contribute to the progression of HIV has been of increased interest to health professionals in recent years. Social scientists as well as medical professionals have begun to offer evidence that certain psychosocial co-factors are important in predicting the health status of HIV-positive individuals. A few models have been offered to illustrate the interrelations among psychosocial co-factors and HIV disease. Stress, social support, coping, and mood states constitute the most frequently researched variables in …
Sulfadimethoxine Analysis In Channel Catfish: A Model Drug Residue Monitoring Program For Aquaculture., Calvin Charles Walker
Sulfadimethoxine Analysis In Channel Catfish: A Model Drug Residue Monitoring Program For Aquaculture., Calvin Charles Walker
LSU Historical Dissertations and Theses
Analytical methods for extraction, detection, and quantitation of sulfadimethoxine (SDM) residues in channel catfish muscle and plasma were developed for a tiered residue monitoring program. The plasma and muscle concentrations of SDM and its primary metabolite in channel catfish, 4-N-acetylsulfadimethoxine (N-acetyl SDM), were determined in SDM medicated fish. Drug extraction methods using matrix solid phase dispersion (MSPD), drug screening methods using enzyme-linked immunoassay (ELISA), and drug residue quantitation methods using high pressure liquid chromatography (LC) are presented. All methods were developed for the simultaneous extraction and analysis of the parent compound and metabolite. MSPD extracts of muscle or plasma were …
Relationship Between Vegetation And Soil Formation In A Rapidly Submerging Coastal Marsh, J. A. Nyman, R. D. Delaune, H. H. Roberts, W. H. Patrick
Relationship Between Vegetation And Soil Formation In A Rapidly Submerging Coastal Marsh, J. A. Nyman, R. D. Delaune, H. H. Roberts, W. H. Patrick
Faculty Publications
In a Terrebonne Basin (Louisiana) marsh, Cs dating indicated that vertical accretion (0.98cm yr ) was extremely rapid relative to other marshes, but insufficient to counter submergence (1.38cm yr ). Mineral and organic matter accumulation were similar to that in other marshes. This marsh is threatened by a positive feedback loop of plant flooding stress and inadequate vertical accretion. Inadequate plant growth limits vertical accretion, which further increases flooding and decreases plant production. Thus plant production partly determines the degree of submergence some coastal and estuarine marshes will tolerate. -from Authors 137 -1 -1
The Poetry In-Between: Presence And Absence In Whitman, Rimbaud, And Hopkins., Jonathan Flint Alexander
The Poetry In-Between: Presence And Absence In Whitman, Rimbaud, And Hopkins., Jonathan Flint Alexander
LSU Historical Dissertations and Theses
"The Poetry In-Between: Presence and Absence in Whitman, Rimbaud, and Hopkins" analyzes three major nineteenth-century poets and their development of a poetics which has as its chief focus of concern the issue of presencing an eternal and universal "Other" by which to assess self identity. After the Kantian critique and the seeming reduction of human knowledge to phenomenal perceptions, early nineteenth-century poets and theorists feared the entrapment and isolation of the self in subjective awareness. The romantics, such as Friedrich Schlegel, sought ways to overcome such alienating subjectivity and ultimately conceived of the poet as a privileged spokesman and arbiter …
Studies On Hepatitis A Virus: I. Expression Of Viral Capsid Peptides In Escherichia Coli And Their Application For An Immunoassay. Ii. Development Of An Assay For Viral Detection By Anti-Hav Antibodies., Luis Antonio Baez
LSU Historical Dissertations and Theses
A panel of eleven monoclonal antibodies of the IgG and IgM class were developed against the HM 175 strain of the Hepatitis A virus. Immunoglobulin specificity for the Hepatitis A virus was determined and the antibodies were applied toward the development of an antigen capture assay linked to a nucleic acid amplification step (RT-PCR), for detection of the Hepatitis A virus. The neutralizing mouse monoclonal antibody and a rabbit polyvalent serum were assessed as capture antibodies for the immunoaffinity stage of the assay. Fifty microliter microfuge tubes were coated with the capture antibody. Formalin inactivated HAV was diluted to concentrations …
Effects Of Subtitled Video During Transactional Task Practice On Oral Communicative Performance Of Fifth Semester College Students Of French Learning With Multimedia Courseware., Isabel Borras
LSU Historical Dissertations and Theses
The purpose of this study was to investigate the effectiveness of subtitled video during transactional task practice on oral communicative performance of fifth semester college students of French learning with multimedia courseware. Task practice was provided by the multimedia package, "Practicing Spoken French" (PSF) specifically designed by the researcher for this study. The package, which integrates HyperCard and videodisc technologies, allows for individually customized practice of two video-based oral tasks, description and narration. Drawing on Salomon's theories about the effects of media coding elements and perception of task on cognition and learning, the study used a 2 x 2 factorial …
Peasant Subsistence In Northwestern Haiti: Geography, Cultural Ecology, And Rural Development., Edward Cameron Britton
Peasant Subsistence In Northwestern Haiti: Geography, Cultural Ecology, And Rural Development., Edward Cameron Britton
LSU Historical Dissertations and Theses
Geographers are challenged to explain "the why of where." This study grapples with "whys" of peasant subsistence in contemporary rural Haiti. Cultural ecology, one of the fundamental themes in cultural geography, examines the interplay between cultural traditions and the realities of subsistence in a given physical environment. Haitian cultural traditions derive from a rich melange of folkways from West Africa, Indian Middle America, and Western Europe. The land is semiarid and hilly in Northwestern Haiti, the poorest, driest, and most sparsely settled of Haiti's provinces. Crops and livestock betray traditional preferences in foodways, and reveal much about the constraints of …
A Southern Social Ethic: Political Economy In The Nineteenth-Century South. Mississippi, 1840-1910. (Volumes I And Ii)., Bradley G. Bond
A Southern Social Ethic: Political Economy In The Nineteenth-Century South. Mississippi, 1840-1910. (Volumes I And Ii)., Bradley G. Bond
LSU Historical Dissertations and Theses
As a study of the southern social ethic, this work discusses some of the most lasting themes of southern historiography: race and class, continuity and discontinuity. The social ethic might best be defined as a collection of ideas, at times contradictory, that suggest southerners' concepts of life in a good republic, citizenship, and proper economic behavior. It also examines the reality of life in a rural state as it experienced the process of modernization. The first third of the dissertation offers a definition of the social ethic. Liberty and virtue, white southerners believed, inhered in all who avoided enslavement, the …
Comparisons Of Spontaneous Mutation Rates And Estimates Of Numbers Of Cell Divisions In The Germlines Of Three Metazoans., Joni Boncille Drost
Comparisons Of Spontaneous Mutation Rates And Estimates Of Numbers Of Cell Divisions In The Germlines Of Three Metazoans., Joni Boncille Drost
LSU Historical Dissertations and Theses
Germline mutation rates measured per generation per locus in three species of Metazoa are used to address questions of the constancy of mutation rates per generation, per year and per germcell division. Null mutation rates per generation are: 3.6 $\times$ 10$\sp{-6}$ for human X-linked disease, 8.9 $\times$ 10$\sp{-6}$ for Specific Locus Test (SLT) in male mice, 3.2 $\times$ 10$\sp{-6}$ for SLT in female mice, 8.0 $\times$ 10$\sp{-6}$ for SLT in male Drosophila melanogaster (D.m.), 1.6 $\times$ 10$\sp{-6}$ for SLRL test in male D.m. and 2.0 $\times$ 10$\sp{-6}$ for SLRL test in female D.m. They were found to be very similar …
New Approaches And Techniques For Drawing Lines On Raster Devices., Philip Arthur Graham
New Approaches And Techniques For Drawing Lines On Raster Devices., Philip Arthur Graham
LSU Historical Dissertations and Theses
Two basic approaches to drawing lines on raster devices are discussed and improved upon. The first is the recursive bisection algorithm, a method recently proposed by John Rankin, which uses a fractal approach to draw lines. The second is the double-step algorithm, a method proposed by Xiaolin Wu and Jon Rokne, which is based on the traditional Bresenham approach to drawing lines. Although a number of line drawing algorithms exist, the algorithms presented are of interest because the double-step algorithm is one of the fastest line drawing algorithms. Furthermore, since lines are self-similar and fractals have been found to be …
Disclosure Decisions And Patterns After A Near-Death Experience., Regina M. Hoffman
Disclosure Decisions And Patterns After A Near-Death Experience., Regina M. Hoffman
LSU Historical Dissertations and Theses
As a dimension of privacy regulation, self-disclosure is the means through which individuals manage the psychological boundary area between the inner, private world and the outer, public world of interaction with others. In communication research, a gap has been noted between self-disclosure as defined by scientific method and self-disclosure as it is experienced by people in their daily life. This study reduces that gap by investigating disclosure decisions about a major life experience through the thick perspectives of the individuals themselves. Disclosure is examined as a context-embedded communicative process. To provide a thick description of disclosure decisions, an ethnographic methodology …
The Death Of A Dialect: Brule Spanish In Ascension Parish, Louisiana., Charles Edward Holloway
The Death Of A Dialect: Brule Spanish In Ascension Parish, Louisiana., Charles Edward Holloway
LSU Historical Dissertations and Theses
The goals of this dissertation are: (1) to describe the Brule dialect, (2) to investigate aspects of language death, and (3) to provide important information to the area of Hispanic dialectology. The vestigial Spanish dialect spoken by the Brule dwellers of Ascension Parish, Louisiana is at the brink of linguistic extinction. The "Brule dwellers" have remained isolated from other Spanish-speaking groups since they arrived in Louisiana from the Canary Islands in the late 1700's. The specific phonological, morphological and syntactic characteristics of this unique variety of Spanish are documented here. Although the close relationship of Brule Spanish to the dialects …