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Mechanisms Of Resistance To Aflatoxin Accumulation By Aspergillus Flavus In Maize Genotype Gt-Mas:Gk., Baozhu Guo Jan 1995

Mechanisms Of Resistance To Aflatoxin Accumulation By Aspergillus Flavus In Maize Genotype Gt-Mas:Gk., Baozhu Guo

LSU Historical Dissertations and Theses

Maize genotype differences in aflatoxin production were observed when kernels of resistant GT-MAS:gk and thirteen susceptible commercial hybrids were inoculated with Aspergillus flavus using a laboratory kernel screening assay. GT-MAS:gk supported the lowest levels of aflatoxin in both intact and endosperm-wounded kernels. Treating intact kernels with KOH effected substantial increases in aflatoxin accumulation in GT-MAS:gk, but only marginal increases in Pioneer 3154. Removing wax from the surface of GT-MAS:gk kernels greatly increased aflatoxin accumulation. These results indicated that GT-MAS:gk resistance was associated with an intact pericarp (wax and cutin layers), acting as a physical barrier, along with internal biochemical factors. …

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Remediation Of Contaminated Soils Using A Plant Based Surfactant., Raghava Rao Kommalapati Jan 1995

Remediation Of Contaminated Soils Using A Plant Based Surfactant., Raghava Rao Kommalapati

LSU Historical Dissertations and Theses

A plant based surfactant extracted from fruit pericarps of Sapindus mukurossi (Ritha) is employed for the first time to investigate remediation of contaminated soils. A method for preparing natural surfactant solutions was developed and the solutions were characterized followed by generation and characterization of colloidal gas aphron (CGA) suspensions produced with these solutions. The variation of hexachlorobenzene (HCB) solubility with natural surfactant solutions was estimated and desorption of HCB from soils was investigated with batch and on-dimensional column experiments. Bioenhancement of soil microorganisms in natural surfactant solutions was studied under both aerobic and anaerobic conditions. The empirical formula for natural …

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Mechanisms Of Chemically-Induced Hepatocarcinogenesis In Western Mosquitofish (Gambusia Affinis)., Jerry Mchugh Law Jr Jan 1995

Mechanisms Of Chemically-Induced Hepatocarcinogenesis In Western Mosquitofish (Gambusia Affinis)., Jerry Mchugh Law Jr

LSU Historical Dissertations and Theses

A need exists for fish sentinel species for monitoring environmental changes. These studies were performed to examine the suitability of western mosquitofish (Gambusia affinis) as a sentinel species and to explore basic mechanisms of hepatocarcinogenesis. Neoplastic lesions were induced in laboratory-reared mosquitofish fry and adults using single pulse exposures to 1 or 10 ppm methylazoxymethanol acetate. Early changes in the liver of exposed fish included severe cytotoxicity, megalocytosis, and widespread apoptosis. Neoplastic changes were found in the liver in approximately 20% of the exposed fish by 8 weeks after exposure and increased to approximately 70% by 41 weeks. These included …

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The Transcendental Element In The Absent Presence., Maggie Burnaman Martin Jan 1995

The Transcendental Element In The Absent Presence., Maggie Burnaman Martin

LSU Historical Dissertations and Theses

"The Transcendental Element in the Absent Presence" analyzes the absent presence, the rhetorical and literary states of being there in the mind of the perceiving individual, though not there physically. It seeks to answer: What does the term "absent presence" mean? Is there a difference between rhetorical and literary absent presences? If so, how is each manifest through the reading process? And, what sustains these absent presences? Evidenced through selected works of Plato, Aristotle, New Testament writers, Sidney, Shakespeare, and Dickinson, the study argues for the intellectually, spiritually, or aesthetically transcendent quality of the absent presence. Any encounter between reader, …

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Henry James And The Process Of Autobiography., Paul S. Nielsen Jan 1995

Henry James And The Process Of Autobiography., Paul S. Nielsen

LSU Historical Dissertations and Theses

James's autobiographies differ from most by dramatizing so extensively the process of recovery and reanimation of memory, the act that signifies autobiographical activity. They therefore reveal a great deal about the generic nature of autobiographical recollection. James's Major Phase, from 1900 to his death in 1916, was chiefly and gloriously autobiographical in purpose and crowns his career with an autobiographical production of astonishing variety, extent, and creativity. The proto-autobiographical material includes biography of a culture (William Wetmore Story and His Friends), travel memoir and cultural analysis (The American Scene), and recapitulation and intimate disclosure of his creative life (the Prefaces …

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The Relationship Between Daily Stress And Neuropsychological Test Performance., Mark Edward Todd Jan 1995

The Relationship Between Daily Stress And Neuropsychological Test Performance., Mark Edward Todd

LSU Historical Dissertations and Theses

Much research has focused on identifying the causes and risk factors associated with Alzheimer's disease (AD). Surprisingly, stress, which has been implicated in the course of a variety of other diseases, has been generally ignored in research on AD despite the fact that several different areas lend indirect support for a relationship between stress and the progression of AD. In this study, the effect of daily stress on the clinical course of AD was examined. Also, given that no study has examined the effects of daily stress on neuropsychological test performance, this relationship was examined as well. Forty-five out-patients with …

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Arsenic Characterization In Soil And Arsenic Effects On Canola Growth., Michael Scott Cox Jan 1995

Arsenic Characterization In Soil And Arsenic Effects On Canola Growth., Michael Scott Cox

LSU Historical Dissertations and Theses

Reactions of soil arsenic with arsenic addition and the effects of soil arsenic on canola were studied because of a lack of information on this subject. The reactions of different pools of soil arsenic to arsenic addition were studied. The effects of soil arsenic and arsenic form and concentration in solution on canola growth and nutrient uptake were also investigated and an attempt to model arsenic uptake with a mechanistic computer model was made. In a solution study, rate of inorganic arsenic did not appear to effect arsenic accumulation in roots and shoots of canola. However, shoot and root arsenic …

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Functional Anatomy Of The Integument And Subcutaneous Structures Of The Head, Neck And Thorax Of The Domestic Turkey, Meleagris Gallopavo., Kumudini Nishantha De Silva Jan 1995

Functional Anatomy Of The Integument And Subcutaneous Structures Of The Head, Neck And Thorax Of The Domestic Turkey, Meleagris Gallopavo., Kumudini Nishantha De Silva

LSU Historical Dissertations and Theses

The microdissection of the integument and subcutaneous structures of the head, neck, and thorax of the Domestic Turkey, Meleagris gallopavo, revealed a complex system with several distinct layers of connective tissue, smooth and striated musculature, and fat bodies (corpora adiposa). These layers show structural variations across the body surface, and the patterns of variation of the different layers correlate with one another. The smooth apterial muscles and the striated subcutaneous muscles complement each other in moving certain feather tracts. Fat tissue is deposited in the dermis as dermal fat, in the Fascia superficialis, and in distinct fat bodies associated with …

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The Evolution Of The Romantic Ballet: The Libretti And Enchanter Characters Of Selected Romantic Ballets From The 1830s Through The 1890s., Tamara Lee Gebelt Jan 1995

The Evolution Of The Romantic Ballet: The Libretti And Enchanter Characters Of Selected Romantic Ballets From The 1830s Through The 1890s., Tamara Lee Gebelt

LSU Historical Dissertations and Theses

With the collapse of Neoclassicism and its worn-out mythological subject matter, the middle class increasingly attended performances previously dominated by aristocratic audiences. New artistic tastes developed; people sought a return to nature and all its mysteries. An interest in folklore, fairy tales, legends, and old literature accompanied a timeless human need for periodic escapism and fantasy. Ballet became a major vehicle for that need. When Marie Taglioni initiated dancing on pointe as the Sylphide in La Sylphide (1832), ballet gained favor, and the Romantic era was born in dance. Ballet flourished, and new scientific methods for training dancers included a …

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A Normative Study Of The Diagnostic Assessment For The Severely Handicapped (Dash) Scale., Martha Lyle Hamilton Jan 1995

A Normative Study Of The Diagnostic Assessment For The Severely Handicapped (Dash) Scale., Martha Lyle Hamilton

LSU Historical Dissertations and Theses

The prevalence of psychopathology in a large institutionalized population (N = 1259) of individuals with severe and profound mental retardation was examined, using the Diagnostic Assessment for the Severely Handicapped (DASH) scale. Over 60% of the sample exhibited at least one form of disorder according to newly established DASH criteria. Forty percent exhibited two or more disorders. Differences between severely and profoundly mentally retarded groups were found, especially in terms of the duration of symptoms. Profoundly mentally retarded persons had significantly more persistent symptoms. Items were examined for frequency and duration, and items of critical importance to diagnosis were identified. …

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Flow Cytometric Analysis Of Avian Blood Cells: Differentiation Of Erythrocytes And Leukocytes By Fluorescence., William Weaver King Jan 1995

Flow Cytometric Analysis Of Avian Blood Cells: Differentiation Of Erythrocytes And Leukocytes By Fluorescence., William Weaver King

LSU Historical Dissertations and Theses

Automated analyzers have revolutionized diagnostic hematology in mammalian species. These commercial instruments utilize flow cytometric technology to enumerate blood cell concentrations. Because of the nuclei present in most non-mammalian erythrocytes and thrombocytes, these instruments are unable to calculate leukocyte counts in birds, amphibians, reptiles, and fish. These investigations sought to determine if three commonly used methodologies in flow cytometry could sufficiently differentiate avian erythrocytes, leukocytes, and thrombocytes, and ultimately form a basis for performing total leukocyte counts. Fluorescein isothiocyanate (FITC) and thiazole orange (TO) were used to stain samples of whole, erythrocyte-, and leukocyte-enriched chicken blood. Although fluorescent microscopic and …

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Attraction Vs. Repulsion: The Narrative Ambivalence Of Gender In Byron's "Don Juan"., Melinda A. Piontek Jan 1995

Attraction Vs. Repulsion: The Narrative Ambivalence Of Gender In Byron's "Don Juan"., Melinda A. Piontek

LSU Historical Dissertations and Theses

Previous studies of gender in Byron's Don Juan, such as those by Susan Wolfson and Louis Crompton, have concentrated primarily on identifying gender ambivalence and attributing that ambivalence to factors outside the text, such as Byron's ambivalence toward his personal homoeroticism as well as to the social attitudes of Regency England toward questions of gender ambiguity. In this dissertation, I propose to turn the critical gaze back to the text in order to go beyond identifying gender ambivalence to track how that ambivalence works within Don Juan. In order to bring into focus the serial and episodic nature of Byron's …

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Changes Of Antioxidants And Fatty Acid Composition In Stabilized Rice Bran During Storage., Taisun Shin Jan 1995

Changes Of Antioxidants And Fatty Acid Composition In Stabilized Rice Bran During Storage., Taisun Shin

LSU Historical Dissertations and Theses

The effects of extrusion temperature and post extrusion holding time, microwave heating, and gamma irradiation on antioxidants and lipid composition in rice bran were studied. Post extrusion holding time had no apparent effect on rice bran stability. Increased holding times reduced (p $<$ 0.05) total vitamin E. Oryzanol concentration was lower (p $<$ 0.05) only with 6 min holding time. Inactivation of lipolytic enzymes was obtained by extrusion temperatures of 120, 130, and 140$\sp\circ$C. Increased extrusion temperatures reduced the retention of vitamin E and oryzanol during storage. An extrusion temperature of 110$\sp\circ$C provided the highest retention of vitamin E and oryzanol during storage although FFA level was higher (p $<$ 0.05) than that of the other extrusion temperatures from 105 to 375 days. There were no significant (p $>$ 0.05) decreases in individual vitamin E vitamers until 4 min microwave heating. After 8 min heating, individual E vitamers decreased significantly (p $$ 0.05) in FFA level by 24 weeks of storage with microwave heating for 12 min. Microwave heating for 4, 8, and 12 min did not provided adequate inactivation of lipolytic enzymes. Total vitamin …

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Encoding Imperialism: Homelessness In American Naturalism, 1890-1918., Janet M. Whyde Jan 1995

Encoding Imperialism: Homelessness In American Naturalism, 1890-1918., Janet M. Whyde

LSU Historical Dissertations and Theses

Homelessness occurs with uncommon regularity in the works of American naturalists, and in each case, the result of a character's homelessness results in a crisis of social identity and self definition. This pattern recurs in the works of the canonically identified naturalists, such as Stephen Crane, Frank Norris, and Theodore Dreiser, as well as other writers who have only been tangentially associated with naturalism--Edith Wharton and Paul Laurence Dunbar, for example. In this study, I analyze the relationship between homelessness as it is represented in the novels and the political debate over the United States's imperialist aspirations at the turn …

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Biogeographic, Ecological, And Evolutionary Aspects Of South American Austral Migration, With Special Reference To The Family Tyrannidae., Robert Terry Chesser Jan 1995

Biogeographic, Ecological, And Evolutionary Aspects Of South American Austral Migration, With Special Reference To The Family Tyrannidae., Robert Terry Chesser

LSU Historical Dissertations and Theses

South American austral migrants are bird species that breed in temperate South America in the southern summer and migrate north, towards or into northern South America, for the southern winter. Austral migration is similar to other avian migration systems in many ways, but its uniquely South American locale is reflected geographically by the relatively short-distance migrations of most austral migrants, and taxonomically by the numerical dominance of the suboscine family Tyrannidae (the tyrant-flycatchers), of which more than 70 species migrate. Detailed examination of the distributions of austral migrant flycatchers revealed that each is to some extent unique, but that discernible …

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The Pathogenesis Of Equine Endometritis Due To Streptococcus Zooepidemicus., Robert Crawford Causey Jan 1995

The Pathogenesis Of Equine Endometritis Due To Streptococcus Zooepidemicus., Robert Crawford Causey

LSU Historical Dissertations and Theses

The ability of uterine isolates of Streptococcus zooepidemicus to resist phagocytosis was explored by the organism's ability to grow in equine fresh blood. Although isolates grew in fresh blood of many horses, any given isolate was killed by blood of certain horses. Killing required leukocytes, heat labile and heat stable components of plasma and appeared to follow phagocytosis of the organism by neutrophils. This led to the working hypothesis that killing was mediated by phagocytosis requiring complement and isolate-specific antibody to variable surface antigens. Variation in killing between horses was attributed to differences in circulating isolate-specific antibody. This is a …

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Using Drawing Tasks To Communicate Ideas About Photosynthesis: A Conceptual Change Strategy For Use In The Elementary School Classroom., Byron Levy Launey Jan 1995

Using Drawing Tasks To Communicate Ideas About Photosynthesis: A Conceptual Change Strategy For Use In The Elementary School Classroom., Byron Levy Launey

LSU Historical Dissertations and Theses

It is difficult for a teacher to determine if a learner has acquired an accurate concept of the topic being taught. Most of the children in this study had sufficient language skills to communicate successfully with their teachers even though they held inappropriate concepts of photosynthesis. This study examined the use of drawing tasks to assess children's ideas related to photosynthesis in an elementary-grade classroom. Two research questions guided the study to determine if this strategy was a valid improvement over traditional methods of classroom instruction. The first question asked if elementary-grade students receiving instruction about photosynthesis would acquire and …

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The Influence Of Subject Sophistication On The Ability To Feign Mild Head Injury Symptoms., Roy Clayton Martin Jan 1995

The Influence Of Subject Sophistication On The Ability To Feign Mild Head Injury Symptoms., Roy Clayton Martin

LSU Historical Dissertations and Theses

The present study examined the effects that personal experience with head injury, task specific instruction and brain injury knowledge have on the ability to feign postconcussion symptoms and neuropsychological performance patterns seen in mild head injured patients. A sample of non-head injured and head injured college students served as the experimental subjects. These subjects were randomly assigned to conditions in which they were told to either do their best, feign deficits consistent with mild head injury without task specific instruction, or feign deficits while given task specific instruction. Subjects were also classified into one of two head injury knowledge groups …

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Seen And Unseen Cities: Embodied Worlds In Epic And The Novel., Eamon G. Halpin Jan 1995

Seen And Unseen Cities: Embodied Worlds In Epic And The Novel., Eamon G. Halpin

LSU Historical Dissertations and Theses

By all accounts, the city has ceased to function as a cosmos, a coherent world which can offer meaning and validity to the lives of its inhabitants. If the ancient city presented the very image of order, the city since the advent of the Industrial Revolution has appeared to us as a jungle, a wilderness, a wasteland, an endless labyrinth--all images which suggest an essentially chaotic space, one which lacks any organizing principle or rationale. Moreover, if the city once offered the individual the greatest possible realization of his freedom, it now appears as the space in which he is …

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A Two Dimensional Vertically Integrated Moving Boundary Hydrodynamic Model In Curvilinear Coordinates., Vibhas Aravamuthan Jan 1995

A Two Dimensional Vertically Integrated Moving Boundary Hydrodynamic Model In Curvilinear Coordinates., Vibhas Aravamuthan

LSU Historical Dissertations and Theses

A two dimensional, vertically averaged, hydrodynamic mathematical model on an adaptive boundary fitted grid is developed. This model addresses numerous drawbacks in the traditional finite difference models and incorporates such features as moving boundaries due to tidal incursion, and the ability to resolve irregular coastline geometries. A semi-implicit finite difference scheme is developed in order to overcome time step restrictions due to the gravity wave stability criterion. An eulerian-lagrangian formulation is used to solve the hydrodynamic equations on unsteady grid systems. The model is applied to study circulation features in Lake Pontchartrain (a medium sized physical system) and to the …

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Solid Removal Schemes And The Rotating Biological Contactor In Recirculating Fish Culture Systems., Aurelio Alfonso Delos Reyes Jr Jan 1995

Solid Removal Schemes And The Rotating Biological Contactor In Recirculating Fish Culture Systems., Aurelio Alfonso Delos Reyes Jr

LSU Historical Dissertations and Theses

The performance of combinations of the Norpac$\sp\circler$ tube RBC with a bead filter (System I), a tube settler (System II), and a sand filter (System III) in pilot scale recirculating systems stocked with tilapia were evaluated and compared. Good fit empirical equations were obtained relating TAN and NO$\sp2$-N (both exponential), and DO (linear) with daily amount of feed. Likewise, linear equations relating bead filter or sand filter pressure with feed, and flow with pressure were obtained. Systems I and II maintained favorable water quality up to the maximum feed load studied of 3 kg/day. System III effectively handled only 1.2 …

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Notions Of Happiness In Rousseau's "Julie"., Peter Buttross Jr Jan 1995

Notions Of Happiness In Rousseau's "Julie"., Peter Buttross Jr

LSU Historical Dissertations and Theses

This enquiry first establishes both the importance and the general meaning of the notions of happiness in Rousseau's corpus. Both private and public happiness are seen as the overarching intent of his life as a thinker. The possibility of a tertiary notion of happiness--between private and public--is presented with the introduction of the subject work Julie; or The New Heloise. Themes considered at some length are passion/virtue, happiness/duty, love/friendship, as well as the notions of Platonic and courtly love--not to mention the dichotomy motion/rest. At the end of Part One of the novel, the lovers are forcibly separated, and the …

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Responses Of Mammalian Cells To Membrane-Disrupting Peptides., Wai Chun Jennifer Lo Jan 1995

Responses Of Mammalian Cells To Membrane-Disrupting Peptides., Wai Chun Jennifer Lo

LSU Historical Dissertations and Theses

Responses of cultured mouse fibroblasts, human erythrocytes, bovine erythrocytes, and liposomes with different phosphatidylcholine (PC) and sphingomyelin (SPM) ratios to both natural and synthetic lytic peptides were characterized. Peptide-induced morphological alterations of the plasma membrane of cells were examined by various light microscopic techniques and scanning electron microscopy (SEM). The ability of natural and synthetic peptides to kill cells and cause increased permeability of liposomes was evaluated using Trypan Blue (TB) dye exclusion assay and fluorescent dye leakage assay, respectively. Differential interference contrast microscopy, SEM, and fluorescence studies revealed characteristic structural and lipid changes in the plasma membrane of lytic …

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Effects Of Dietary Fatty Acid Ethyl Esters On Polar And Nonpolar Tissue Lipids In Red Drum, Sciaenops Ocellatus., Carmen Mayyuk Chan Jan 1995

Effects Of Dietary Fatty Acid Ethyl Esters On Polar And Nonpolar Tissue Lipids In Red Drum, Sciaenops Ocellatus., Carmen Mayyuk Chan

LSU Historical Dissertations and Theses

Red drum juveniles of mean initial weight 9.23 $\pm$ 0.27 g were kept in indoor aquaria. The water was maintained at a salinity of 12 $\pm$ 0.5 ppt. The fish were fed six isonitrogenous (38% crude protein) and isocaloric (1.36 $\times$ 10$\sp4$ kJ/kg), purified diets differing in lipid content, for nine weeks, to investigate the effects of selected dietary essential fatty acids (EFA) on growth performance and tissue fatty acid composition. Each diet, except the low-fat (LF) diet, contained 8% total lipid from (1) menhaden fish oil (MFO), (2) oleate (18:1n-9) supplemented with linoleate (18:2n-6), (3) linolenate (18:3n-3), (4) a …

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Synthesis And Characterization Of Aminoalkylcarbamoyl Cellulosics., Doris Ann Culberson Jan 1995

Synthesis And Characterization Of Aminoalkylcarbamoyl Cellulosics., Doris Ann Culberson

LSU Historical Dissertations and Theses

The synthesis and characterization of chitin analogs based on water soluble amino cellulose derivatives are described. This family of derivatives exhibits properties suitable for applications as biodegradable carriers for pharmacons and agricultural chemicals, and production of high molecular weight polymeric quaternary salts. The chemical and physical properties of the group of amino cellulose derivatives prepared from the amidation of methyl carboxymethyl cellulose or the aminolysis of cyanoethylated cellulose with different diamines are analyzed. Converting the carboxy-methyl cellulose to a methyl ester produces a derivative more receptive to amidation. When the methyl carboxyl-methyl cellulose is treated with an excess of diamines, …

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Daily Variations In Protein Synthesis And Tissue Responsiveness To Growth Hormone, Prolactin And Insulin-Like Growth Factor-I In The Gulf Killifish, Fundulus Grandis Baird And Girard., Zeleke Negatu Jan 1995

Daily Variations In Protein Synthesis And Tissue Responsiveness To Growth Hormone, Prolactin And Insulin-Like Growth Factor-I In The Gulf Killifish, Fundulus Grandis Baird And Girard., Zeleke Negatu

LSU Historical Dissertations and Theses

Circadian variations of $\sp{14}$C-glycine incorporation into protein and the responsiveness of muscle tissue to growth hormone, prolactin and insulin-like growth factor-I were examined in gulf killifish, Fundulus grandis. Incorporation of label during a 5 h period following $\sp{14}$C-glycine injection was tested at different times of day (LD 12:12). Incorporation varied during the day in all the tissues examined (scale, muscle, liver and intestine). The times of peak incorporations differed among the tissues. Scales showed peak incorporation during the dark hours. Peak incorporation by muscle occurred at the onset of light. These patterns were similar in male and female fish. The …

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Investigation In The Theory Of Stochastic Processes., Xiao Liang Li Jan 1995

Investigation In The Theory Of Stochastic Processes., Xiao Liang Li

LSU Historical Dissertations and Theses

In Chapter I of this dissertation, we present a pedagogical introduction to the basic concepts of stochastic theory and review the progress made as well as the outstanding unsolved problems in the field. For the rest of this dissertation, we use a generalized quantum Langevin equation (GLE) approach to investigate various properties of quantum dissipative systems. In Chapter II, calculations for the displacement and random force correlation functions for Brownian motion are generalized to the case of an arbitrary heat bath for a damped harmonic quantum oscillator. The mean square displacement of such an oscillator is then evaluated for both …

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The Habitat, Ecology And Epidemiology Of Eastern Equine Encephalomyelitis In Southeast Louisiana., Kenny L. Samui Jan 1995

The Habitat, Ecology And Epidemiology Of Eastern Equine Encephalomyelitis In Southeast Louisiana., Kenny L. Samui

LSU Historical Dissertations and Theses

Many unanswered questions on the epidemiology and ecology of eastern equine encephalomyelitis (EEE) still preclude a complete understanding of its natural history. Among these are the distribution and epidemic vectors of EEE virus (EEEV) in different regions, habitats, and foci; its inter-epidemic maintenance, and overwintering mechanisms. Phase 1 of a research project designed to find answers to the above questions with regard to southeast Louisiana was conducted over 1992 and 1993, and consisted of several individual studies. These included 3 mosquito studies to determine major horse- and bird-feeding mosquito species that could be the EEEV epidemic vectors in southeast Louisiana; …

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A Formal Methodology For Deriving Purely Functional Programs From Z Specifications Via The Intermediate Specification Language Funz., Linda Bostwick Sherrell Jan 1995

A Formal Methodology For Deriving Purely Functional Programs From Z Specifications Via The Intermediate Specification Language Funz., Linda Bostwick Sherrell

LSU Historical Dissertations and Theses

In recent years, both formal methods and software reuse have been increasingly advocated as a means of alleviating the ills of the software crisis. During this same time period, purely functional programming languages, which have a long history in the realm of rapid prototyping, have emerged as a viable medium for real-world applications. Since these trends are likely to continue, this work describes a methodology that facilitates the derivation of purely functional programs from existing Z specifications. A unique aspect of the methodology is its incorporation of an intermediate specification language (FunZ) during the design phase of software development. Most …

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Cloning, Expression, Primary Structure, And Insertion Mutagenesis Of The Pyruvate Formate-Lyase Enzyme Of Aeromonas Hydrophila., Mohamed Alaa Eldin Abdelalim Awad Jan 1995

Cloning, Expression, Primary Structure, And Insertion Mutagenesis Of The Pyruvate Formate-Lyase Enzyme Of Aeromonas Hydrophila., Mohamed Alaa Eldin Abdelalim Awad

LSU Historical Dissertations and Theses

The pyruvate formate-lyase enzyme (Pfl), a key enzyme in alcoholic fermentation, catalyzes the anaerobic conversion of pyruvate into acetyl-CoA and formate in prokaryotes. The pyruvate formate-lyase gene (pfl) of the facultative anaerobe Aeromonas hydrophila was cloned and sequenced. Subsequently, the Pfl was characterized based on the deduced amino acid sequence and evaluated in vivo following insertion mutagenesis. Finally, the pathogenesis of a defined mutant was compared to wild type A. hydrophila in channel catfish. The pfl of A. hydrophila was identified from two recombinant pBluescript SK(-) clones excised from a $\lambda$ ZAP II phage library. Both clones carried the same …

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