Digital Commons Network^™

Injectable Antioxidant And Oxygen-Releasing Lignin Composites To Promote Wound Healing, Swathi Balaji, Walker D. Short, Benjamin W. Padon, Jorge A. Belgodere, Sarah E. Jimenez, Naresh T. Deoli, Anna C. Guidry, Justin C. Green, Tanuj J. Prajapati, Fayiz Farouk, Aditya Kaul, Dongwan Son, Olivia S. Jung, Carlos E. Astete, Myungwoong Kim, Jangwook P. Jung

Faculty Publications

The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignin to confer antioxidation to the extracellular microenvironments of wounds and to deliver oxygen from the dissociation of calcium peroxide for enhanced vascularization and healing responses without eliciting inflammatory responses. Elemental analysis showed 17 times higher quantity of calcium in the oxygen-releasing nanoparticles. Lignin composites including the oxygen-generating nanoparticles released around 700 ppm oxygen per day at least for 7 days. By modulating the concentration of the methacrylated gelatin, we were able to maintain the injectability of …

Development, Survival, And Fecundity Of Helicoverpa Zea (Lepidoptera: Noctuidae) On Five Weedy Host Plants Common Across The Mid-South Usa, James P. Glover, Maribel Portilla, Gadi V.P. Reddy

Faculty Publications

Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) is a highly mobile and polyphagous pest known to feed on over 300 cultivated and wild host plant species. Larvae cause significant economic damage to several commercial row crops over multiple generations each growing season. The objective of this study was to compare biological fitness of H. zea on 5 weedy host plants commonly found across the mid-south US. Crimson clover (Trifolium incarnatum L.), white clover (Trifolium repens L.), hairy vetch (Vicia villosa Roth), kudzu (Pueraria montana [Lour.] Merr. (all Fabaceae), and honeysuckle (Lonicera japonica Thunb.; Caprifoliaceae) were evaluated as primary food sources for H. …

The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya

LSU Doctoral Dissertations

Carbonic anhydrases (CAs) are zinc-metalloenzymes that interconvert two inorganic carbon (Ci) species, CO₂ and HCO₃^-. In Arabidopsis thaliana, there are eight αCA genes, six βCA genes, three γCA genes, and two γCA-like genes. The majority of CA research in plants has focused on finding a link between CA activity and photosynthesis rates. Since the CA genes are expressed in different plant tissues and multiple CA isoforms are distributed among various organelles of the plant cell, I hypothesize that CAs facilitate CO₂ diffusion among cell compartments and maintain Ci pools for carbon-requiring reactions by interconverting …

Optimization Of Host Cell-Compatible, Antimicrobial Peptides Effective Against Biofilms And Clinical Isolates Of Drug-Resistant Bacteria, Jenisha Ghimire, Robert J. Hart, Anabel Soldano, Charles H. Chen, Shantanu Guha, Joseph P. Hoffmann, Kalen M. Hall, Leisheng Sun, Benjamin J. Nelson, Timothy K. Lu, Jay K. Kolls, Mario Rivera, Lisa A. Morici, William C. Wimley

Faculty Publications

Here, we describe the continued synthetic molecular evolution of a lineage of host-compatible antimicrobial peptides (AMP) intended for the treatment of wounds infected with drug-resistant, biofilm-forming bacteria. The peptides tested are variants of an evolved AMP called d-amino acid CONsensus with Glycine Absent (d-CONGA), which has excellent antimicrobial activities in vitro and in vivo. In this newest generation of rational d-CONGA variants, we tested multiple sequence-structure-function hypotheses that had not been tested in previous generations. Many of the peptide variants have lower antibacterial activity against Gram-positive or Gram-negative pathogens, especially variants that have altered hydrophobicity, secondary structure potential, or spatial …

Assessing The Nutritional Value Of Dubia Roaches (Blaptica Dubia) As A Feeder Insect In Reptiles, Elise Barras

LSU Master's Theses

Dubia roaches (Blaptica dubia) have emerged on the market as a high protein and low-fat alternative to other feeder insects for captive reptiles. However, much like other insects, dubia roaches naturally have an inverse calcium to phosphorus (Ca:P) ratio. Offering insects to reptiles that are deficient in calcium can lead to nutritional disease (e.g., nutritional secondary hyperparathyroidism). For dubia roaches to be a valuable feeder insect, their Ca:P ratio must be corrected. Several studies have assessed their nutritional composition, but no studies have manipulated the calcium content of these insects. Using a high calcium (8.3% calcium, dry matter) …

Influence Of Metabolic Interventions On Animal Aging And Lifespan, Bhaswati Ghosh

LSU Doctoral Dissertations

Aging is associated with metabolic rewiring. Most lifespan-extending longevity interventions target metabolic pathways. Genetic or pharmacological metabolic approaches that mediate longer lifespan and healthspan, are often linked to detrimental effects on human health. Here, I show that supplementation of botanical extracts from Artemisia scoparia (SCO), dramatically increases animal lifespan. Mechanistically, this lifespan extension is dependent on AMPK-DAF-16 signaling via inhibition of the insulin pathway. In SCO-fed worms, longer lifespan was accompanied by higher fat storage and improved resiliency to age-related physiological stress. Notably, DAF-16, the master regulator of longevity, activated the fatty acid desaturation processes, which promoted the accumulation of …

Cell-Specific Role Of Il4rα Signaling In Allergic Asthma, Ishita Choudhary

LSU Doctoral Dissertations

IL-4 and IL-13 play a critical role in allergic asthma pathogenesis via their common receptor, i.e., interleukin 4 receptor alpha (IL4Rα). However, the cell type-specific role of IL4Rα-mediated signaling in allergic asthma has remained unclear. We hypothesized that the role of IL4Rα signaling in allergic asthma is cell-type specific. To test our hypothesis, we challenged myeloid cell-specific IL4Rα-deficient (LysMcre^+/+/IL4Rα^fl/fl), myeloid cell-specific IL4Rα-sufficient (LysMcre^+/+/IL4Rα^wt/wt), airway epithelial cell-specific IL4Rα-deficient (CCSP-Cre⁺/IL4Rα^fl/fl), and airway epithelial cell-specific IL4Rα-sufficient (CCSP-Cre^-/IL4Rα^fl/fl) mice with either mixed allergens (MA) or normal saline three …

Evaluation Of Reproductive Phenology, Space Use, And Ecology Of The Eastern Wild Turkey (Meleagris Gallopavo Silvestris) In West-Central Louisiana, Chad Argabright

LSU Master's Theses

Nest site selection is a driving demographic force behind eastern wild turkey (Meleagris gallopavo silvestris) populations. However, previous research was likely not focused on the actual time of nest site selection, considering that nest site selection is likely only able to occur on the day of the first egg being laid. My objective was to determine if selection for any vegetation characteristics was occurring on the first day of laying. I estimated the path taken from the roost to the nest on the first day of egg laying (i.e., laying path) using GPS data collected from 164 unique …

Effects Of Stereotypes On Black Women Audiences, Darian M. Shorts

LSU Master's Theses

This study focuses on the effects that televised racial stereotypes have on the self-perception of viewers who identify as Black women. This paper lists three commonly used stereotypes for Black women in television and provides detailed background and analysis of each. There were three goals that I wanted to achieve with this study. The first goal of this study was to measure the amount of stereotyped entertainment these specific viewers consume. The second goal of this study was to understand the positive and negative effects that racial stereotypes have on Black women. The last goal of this study was to …

20-Year Assessment Of Total Suspended Sediment (Tss) Variability In Barataria Bay From Modis Ocean Color Using A Combination Of Adaptive Semi-Analytical And Neural Network Algorithms, Bijaylaxmi Sahoo

LSU Master's Theses

Barataria Bay, a hydrologically dynamic basin in the northern Gulf of Mexico, exhibits a distinct spatio-temporal distribution of total suspended sediment (TSS). However, studies on sediment distribution are limited by availability of in-situ data as well as the limitation of ocean color algorithms for suspended sediments in shallow optically complex waters. Barataria Bay TSS concentration profile is complex, influenced in the upper basin by fresh water sources such as the Davis Pond Freshwater Diversion, and in the lower bay by the river plume and marine influence near the mouth of the bay. To efficiently study the sediment dynamics in the …

The Influence Of Native Language And Sentence Form On Memory Of Motion Events, Stephanie L. Lopez

LSU Master's Theses

This study utilized four experiments to investigate the extent to which native language influences memory in accordance with linguistic relativity. In Experiment 1, monolingual English speakers and Spanish/English bilinguals were divided into a verbal encoding condition and a verbal suppression encoding condition and watched motion events of low or high physical salience. Participants engaged in a recognition memory task followed by an event memory similarity judgment task. In Experiment 2, native monolingual English speakers were divided into an English-like (or manner-on-verb) description group, a Spanish-like (or path-on-verb) description group, mimicking the language groups of Experiment 1 respectively, and a verbal …

Cannabis Use Across The Menstrual Cycle: The Impact Of Negative Affect And Coping Motives, Paige Morris

LSU Master's Theses

Women appear to use more cannabis during the premenstrual and menstrual phases of the menstrual cycle, presumably because women use cannabis to manage increases in negative affect associated with these phases. Yet little work has tested this hypothesis or examined individual difference variables associated with greater coping-motivated cannabis use. The current study tested whether state negative affect interacts with coping motives to predict greater cannabis use during the premenstrual and/or menstrual phases and whether trait anxiety or difficulties with emotion regulation moderate these relations. Female undergraduate students with regular monthly menstrual cycles and who were not using hormonal contraceptives ( …

The Non-Apprehension Of Selfhood And Metaphysical Freedom, Christopher Rinderle

LSU Master's Theses

In this work I take on one of the most axiomatic assumptions humans possess. The sense of self is embedded so deeply inside each one of us that to question it seems utterly ridiculous. Fortunately, I do not outright reject the notion of selfhood. I investigate what it is and is not through multiple angles – ancient and contemporary – which leads to investigations of free will and responsibility.

In the first chapter, I discuss the Buddhist anātman or non-self. I argue that the historical Buddha did not endorse the idea that the self must be overcome through enlightenment – …

Characterizing Marr Homologs That Mediate Bacterial Fitness And Survival, George Chukwuemeka Nwokocha

LSU Doctoral Dissertations

Plant disease outbreaks are on the rise, threatening food security in many parts of the globe. To address these enormous challenges, new intervention strategies like bio-controls are being used as an important sustainable alternative to mitigate the negative effects of pathogens and increase the quality and productivity of agricultural crops. MarR family transcriptional regulators have been studied for their involvement in regulating bacterial virulence genes that help colonize plant hosts and therefore could serve as targets for biocontrol. The transcriptional regulator PecS, a member of the MarR family, is encoded by select bacterial pathogens and we show here that PecS …

The Abolitionist Spectator: Resistant Readings Of Punishment, Rehabilitation, And Reform At The Louisiana State Penitentiary, Kathryn Marie Morris

LSU Doctoral Dissertations

For the majority of the twenty-first century, Louisiana has been the global leader in rates of incarceration. Despite its prevalence, many people encounter prisons and punishment only through representations in movies, television, and the news, remaining distant from the actual processes of punishment and prisons built in remote areas. The Louisiana State Penitentiary at Angola, however, is unique in the number of opportunities it presents for the public to enter the prison for large-scale events like the famous Angola Prison Rodeo, select drama club performances, or to visit the Angola Prison Museum. These opportunities are often read cynically, as moments …

The Regulation Of Autoimmunity Genes In Pancreatic Β-Cells By Nf-Κb, Thomas Malone Martin

LSU Doctoral Dissertations

Type 1 diabetes (T1D) is an autoimmune disease where the immune system targets the pancreatic islet beta-cells, which are responsible for insulin production and secretion, leading to a state of insulin insufficiency. Many of the molecular details of disease onset and progression remain poorly understood, including key aspects of cytokine signaling. Therefore, we have examined some of the consequences of inflammatory cytokine signaling as a critical module relevant to T1D. Using interleukin-1 (IL-1) signaling as a well-defined paradigm for inflammation, we investigated key components of this signaling pathway in pancreatic beta-cells. We identified ICAM-1, one of the proteins expressed in …

Exploring The Interactive, Linguistic, And Conceptual Dimensions Of Parent Input And Their Role In Children's Pragmatic Development, Nickolas T. Day

LSU Master's Theses

The development of children’s pragmatic language, which can be defined as conversational competence in social contexts, has been attributed to variation in the language they hear in their environment. When studying the impact language input has on pragmatic development, it is important for clinicians and linguists to measure both the quantity and quality of this language input. Measures of quality, however, are much more relevant when studying the development of pragmatic language, as discussed in Zhang (2020) and Hirsh-Pasek et al. (2015). Rowe and Snow (2016) discussed three dimensions of language quality: interactive, linguistic and conceptual. There is a current …

Identifying Key Language Research Priorities In Autistic Children According To Parents, Taylor Hale

LSU Master's Theses

The lack of involvement of the autistic community and its stakeholders in autism research has led to a call to action. At present, autism researchers have limited knowledge about stakeholders’ priorities for research. The current study aimed to bridge the disconnect between the extant autism research and the recent neurodiversity movement by surveying key stakeholders – parents of autistic children – on their perspectives on autism research that focuses on language. Twenty-six parents of autistic children completed an online survey on their views on autism language research with an option to participate in a follow-up interview. Six parents participated in …

Gulf Sturgeon Mesohabitat Use And Movement In The Lower Pearl River Louisiana-Mississippi, Amanda Popovich

LSU Master's Theses

After spring migrations to natal rivers Gulf Sturgeon Acipenser oxyrinchus desotoi reside in freshwater holding areas prior to fall emigrations to nearby estuaries. Limited published information on the physical habitat characteristics of these holding areas is available, especially for juvenile fish in the western portion of the Gulf Sturgeon’s distribution. To further investigate freshwater occupancy and movement patterns, 32 acoustic receivers were deployed from late April to October of 2021 and 2022 in 1600-m reaches distributed throughout the lower Pearl River, Louisiana-Mississippi, the westernmost river within their distribution. Generalized linear mixed models and multi-state models were used to determine what …

Effect Of Furosemide Administration On Plasma Analytes And Urine Enzyme Excretion In Two Reptilian Species, Kathryn Metcalf

LSU Master's Theses

Renal disease is a common ailment of captive reptiles that is often closely linked to chronic, subclinical states of dehydration. Currently, the diagnosis of renal disease in reptiles is poorly characterized and often relies on invasive diagnostic techniques (e.g., renal biopsy) for definitive diagnosis. A potential outlet for the further characterization of renal dysfunction in reptiles is the induction of dehydration by furosemide therapy. Furosemide is a loop diuretic that is commonly used in mammalian patients for the treatment of congestive heart failure. The utility of furosemide in reptiles is poorly characterized, however, both experimental and clinical utility of this …

The Possibility Of Relationships With Others, Joshua Severud

LSU Master's Theses

Levinas offers a rich philosophical insight into the kind of responsibility and ethics that we must have for the Other. This involves a certain conception of what it means to be hospitable which turns out to be impossible. In order to talk about how this impossible relationship can occur, I use Heidegger’s description of the existential Being-with structure and Derrida’s conception of the event in order to make sense of how this Levinasian relationship can possibly exist in spite of—or thanks to—its impossibility.

News(?)Papers: A Typology Of Fake News, 1880-1920, Olivia G. Romaguera

LSU Master's Theses

“Fake news” is a malleable concept. It can be beaten and reshaped to fit many different contexts. One widely accepted definition of fake news is false information purporting itself to be factual. Another is information that is factual but called “fake” in order to discredit it. Concern over the spread of fake news increased in recent years. But preoccupation with what is happening today has left a gap in our understanding of the phenomenon, specifically its roots in the past. “Fake news” was present when news technology was relatively primitive; it is not essentially a function of such modern advancements …

Natural Lights & Natural Rights: The Problem Of The New Classical Natural Law Theory, Charles Neville Cacciatore

LSU Master's Theses

The present work examines the natural law jurisprudence of John Finnis. It argues that Finnis’s teaching is a genuinely new natural law theory. Finnis’s jurisprudence is not a re- presentation of the jurisprudence of St. Thomas Aquinas because its central element—a doctrine of natural rights—is a departure from Aquinas’s natural law teaching. In support of these claims, the present work relies upon the scholarship of Ernest L. Fortin, A.A. Following Fr. Fortin, it presents an understanding of the natural law that endorses a clear distinction between natural right and natural rights—between premodern political philosophy and modern political philosophy.

The Nature And Role Of Phenomenology In Hegel And Heidegger, Gabriel W. Connor

LSU Master's Theses

In this work I compare Hegel and Heidegger’s conception of phenomenology and its role in their thinking. Though these two thinkers are not often examined from this angle, and though there is controversy surrounding just how phenomenological each thinker might actually be, an examination of the two thinkers in this regard serves to identify interesting connections between Hegel and Heidegger while also raising questions about phenomenology in general. In short, I seek to establish that phenomenology in both Hegel and Heidegger is not adequately understood unless it is placed in the context of each thinker’s conception of human freedom along …

Switch-Like Behavior Of Lysosomes And Vcp Supports Spermatocyte Health And Development In Drosophila, Tyler James Butsch

LSU Doctoral Dissertations

Changes to societal norms, such as the educational, marital, and child-bearing expectations have coincided with significant increases in infertility worldwide. Surprisingly, male infertility is responsible for approximately half of all infertility cases worldwide. Thus, a better understanding of sperm development, and how it is affected by age, may permit the design and application of therapeutics to treat various cases male infertility. Here, I have found that lysosomes acidify as germ cells enter the spermatocyte stage. Once active, lysosomes turn over E-cadherin, and likely other proteins, to support plasma membrane stability. Notably, aging negatively impacts lysosome acidification, which can be reversed …

Svip Dependent Tubular Lysosomal Restoration: A New Direction In Vcp Diseases, Ankita Basu

LSU Doctoral Dissertations

Multisystem Proteinopathy-1 (MSP-1) is a rare, autosomal dominant, degenerative disease resulting from single amino acid mis-sense mutations in the AAA+ ATPase, Valosin containing protein (VCP). Patients with VCP mutations vary widely in their pathology and clinical penetrance, making it difficult to devise effective treatment strategies. To investigate the pathology of VCP- related disease, we engineered CRISPR knock-in Drosophila models for nine MSP-1 associated VCP mutations. These mutant animals mimic human MSP-1 disease phenotypes, including progressive mobility decline and accumulation of protein aggregates, making them excellent models to study the pathology of VCP associated mutations and test potential treatment strategies. At …

Contribution Of The Microbiome On Reproductive Outcomes During Pregnancy, Kalie Beckers

LSU Doctoral Dissertations

A series of translational comparative studies were performed to investigate the effects of the maternal microbiome on reproductive function and outcomes. With the launch of the human microbiome project in 2007, the next generation sequencing of microbiomes is booming. It is not only in the human medicine field, but also in veterinary species. This study specifically investigates the fecal microbiome in blood pressure high sublime 5 (BPH/5) mouse model that spontaneously develops preeclampsia (PE) and obesity. Additionally, the equine reproductive tract microbiome was explored and compared to microbiome of other host body sites. The BPH/5 mouse is a translational model …

Phonic Faces As A Method For Improving Decoding For Children With Persistent Decoding Deficits, Chantal H Farag Hanna

LSU Master's Theses

Background: Decoding is a foundational skill for reading, contributing to both reading fluency and comprehension (Lyon et al., 2003). Visual enhancements of alphabetic letters such as shaping letters to resemble words beginning with that sound (e.g., “f” drawn as a flower) (Handler & Fierson, 2011) and associating photographs of lips producing the sounds (Lindamood & Lindamood, 1998) have been shown to improve decoding skills. This study investigated whether a more direct pictured association using faces with alphabet letters placed in the mouth to cue speech sounds, termed Phonic Faces (Norris,2001), would enable students with persistent decoding impairment to acquire orthographic …

Snacking Consumption Among Adults In The United States: A Scoping Review, Jean Pierre Enriquez, Elizabeth Gollub

Faculty Publications

Snacks are a staple of the American diet, contributing to approximately 20% of energy intake. Most U.S. adults consume one to three snacks/day, yet few reviews have focused on snacking among this population. This scoping review was conducted to characterize snacks and snacking occasions among U.S. adults to further inform healthy eating practices. The protocol was prepared following the PRISMA-Extension for Scoping Reviews. Three web databases were used to identify articles using snacking or eating occasions as primary or secondary outcomes among U.S. adults. A search strategy was developed using subject headings, truncation, and phrase searching in the title and …

Spring 2023 Catalog, Lsu Press

LSU Press Seasonal Catalogs

No abstract provided.