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The Rhetorical Myth Of The Athlete As A Moral Hero: The Implications Of Steroids In Sport And The Threatened Myth, Karen L. Hartman Jan 2008

The Rhetorical Myth Of The Athlete As A Moral Hero: The Implications Of Steroids In Sport And The Threatened Myth, Karen L. Hartman

LSU Doctoral Dissertations

This research analyzes changes in the rhetoric of a sustaining myth in order to better assess what happens when a myth is threatened. By examining American sport and its current struggle to withstand the widespread use of steroids, the author investigates how public discourse about the scandal turns athletes from mythical heroes to cheaters. The author begins by explicating the rhetorical construction of the athlete as a moral hero in America and how this myth is perpetuated today. The author then examines how steroids threaten the myth of the moral athlete and uses Major League Baseball as a case study …

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A Rhetoric Of Existentialism, Zachary Gershberg Jan 2008

A Rhetoric Of Existentialism, Zachary Gershberg

LSU Doctoral Dissertations

Existentialism is often viewed as a morbid philosophy but adapting it to a rhetorical framework reveals a consistent interest in the ontological function of communication. The exchange of discourse and symbols is what ties humans together and existentialism examines the meaning that abounds in life as opposed to attempting to discover the meaning of life. As a rhetorical construct, existentialism provides a critical and unique view of agency and edification as a method of rhetorical practice.

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Syntheses Of Trimethylamine- And Phosphonate- Substituted Carboranylporphyrins For Application In Boron Neutron Cancer Therapy, Michael William Easson Jan 2008

Syntheses Of Trimethylamine- And Phosphonate- Substituted Carboranylporphyrins For Application In Boron Neutron Cancer Therapy, Michael William Easson

LSU Doctoral Dissertations

Chapter 1 contains an introduction to porphyrins and carboranylporphyrins, including their physical properties, history, methods of synthesis, medicinal applications, and an overview of recently published compounds. Chapter 2 contains the total synthesis of a series of trimethylamine- and phosphonate-substituted carboranylporphyrins for application in boron neutron capture therapy (BNCT), including characterization by proton nuclear magnetic resonance spectroscopy, matrix-assisted time-of-flight mass spectroscopy, high resolution mass spectroscopy, UV-Vis spectrophotometry, X-ray crystallography, and melting point apparatus. Chapter 3 contains synthetic methods which were investigated as alternate routes to porphyrin synthesis and functionalization. Chapter 4 presents the in vitro biological evaluation of trimethylamine- and phosphonate-substituted …

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Bioanalytical Methods For Studies Of Homocysteine And Novel Cardiovascular Disease Indicators, Arther T. Gates Jan 2008

Bioanalytical Methods For Studies Of Homocysteine And Novel Cardiovascular Disease Indicators, Arther T. Gates

LSU Doctoral Dissertations

This dissertation explores the development of analytical methods for studies of CVD biomarkers and related biomolecular indicators. Initially, spectroscopic studies were conducted to investigate the chemical reactivity of homocysteine (Hcy), an independent CVD risk factor and serological biomarker. Consequently, we proposed an alternate theory for in vivo Hcy clearance based on spontaneous pyridoxal tetrahydrothiazine (PT) formation from Hcy and pyridoxal. The validity of PT-assisted Hcy clearance was further evaluated by use of capillary electrophoretic methods, which allowed rapid monitoring of protein oligomerization in PT-protein reaction mixtures. The results of these studies suggest that PT formation is a plausible mechanism for …

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Catalytic Synthesis Of Ethanol From Biomass-Derived Syngas, Adefemi Adelanwa Egbebi Jan 2008

Catalytic Synthesis Of Ethanol From Biomass-Derived Syngas, Adefemi Adelanwa Egbebi

LSU Doctoral Dissertations

Syngas produced by gasification of biomass or coal can be converted directly to ethanol and higher alcohols by processes based on heterogeneous catalysts. Ethanol can be used as a neat fuel or fuel additive or as a hydrogen carrier. Rhodium-based catalysts have been found to be most selective for the formation of C2 oxygenates from the hydrogenation of CO when suitably promoted. Here, we explore the effects of Mn, Fe, and Li promoters on Rh/TiO2. Rh-TiO2, Rh-Li/TiO2, Rh-Mn/TiO2, Rh-Mn-Li/TiO2 and Rh-Mn-Li-Fe/TiO2 catalysts were tested for the hydrogenation of CO …

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Concentration Of Phosphorylated Proteins Using Modified Pmma Microanalytical Devices, Jowell Guerra Bolivar Jan 2008

Concentration Of Phosphorylated Proteins Using Modified Pmma Microanalytical Devices, Jowell Guerra Bolivar

LSU Doctoral Dissertations

This work describes the application of PMMA-based microanalytical devices for the affinity-type preconcentration of posttranslational modified proteins (PTMs). The choice of poly(methyl methacrylate), PMMA, is based on its biocompatibility, its functional methyl ester group for potential modification, and its extensive applications to create biological microelectromechanical systems (BioMEMS). Developing methodologies for preconcentration of PTMs is important for cancer diagnosis due to PTMs’ influence in the regulatory mechanism underlying the early stage of apoptosis or regulated cell death. Towards this goal, nitroavidin which can reversibly binds to biotin (and biotinylated proteins), was prepared using reported procedure and was characterized using several techniques …

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Novel Methods For The Control Of Phlebotomine Sand Flies (Diptera: Psychodidae), Thomas Michael Mascari Jan 2008

Novel Methods For The Control Of Phlebotomine Sand Flies (Diptera: Psychodidae), Thomas Michael Mascari

LSU Doctoral Dissertations

In arid and semi-arid parts of the Old World, Phlebotomus paptasi is a significant biting pest of man and is the primary vector of Leishmania major, the causative agent of zoonotic cutaneous leishmaniasis (ZCL). Phlebotomus papatasi exhibits a close association with the burrowing rodents that serve as the reservoirs of L. major. Rodent burrows are considered to be the primary habitat of immature P. papatasi in ZCL foci, and sand fly larvae have been observed feeding on rodent feces. In laboratory studies, five insecticides (diflubenzuron, novaluron, methoprene, pyriproxyfen, or ivermectin) were incorporated into the diet of Syrian hamsters and evaluated …

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The Synthesis Of Water Soluble N-Acyl Chitosan Derivatives For Characterization As Antibacterial Agents, Lakia Monique Champagne Jan 2008

The Synthesis Of Water Soluble N-Acyl Chitosan Derivatives For Characterization As Antibacterial Agents, Lakia Monique Champagne

LSU Doctoral Dissertations

Currently, natural polysaccharides are being utilized increasingly in the markets because they exhibit biodegradability, biocompatibility, versatility, and are found abundant in nature. The diversity of natural polysaccharides provides the chemist with a broad spectrum of raw materials that can be used in many biological applications. Chitosan is a natural polysaccharide that possesses excellent biological properties. It has been recognized for its antibacterial activity, in that it is destructive towards the bacterium Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The antibacterial property of chitosan can be enhanced by changing the hydrophobic/hydrophilic nature of the polysaccharide backbone. This research entails …

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Language Learning Through Storybook Reading In Headstart, Patricia Minnis Brazier-Carter Jan 2008

Language Learning Through Storybook Reading In Headstart, Patricia Minnis Brazier-Carter

LSU Doctoral Dissertations

The purpose of this study is to explore whether storybooks designed to elicit talk about letters and sounds, termed "alphabet-storybooks" will generate more print referencing behaviors from Head Start teachers than traditional storybooks, and if there is a concomitant positive impact on the learning of the children in these classrooms. In addition, the meaning reference behaviors of adults and impact on children also will be measured to determine if meaning is sacrificed at the expense of print referencing.

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Cryopreservation Of Adipose Derived Adult Stem Cells And Multidimensional Cell Sheets, Sreedhar Thirumala Jan 2008

Cryopreservation Of Adipose Derived Adult Stem Cells And Multidimensional Cell Sheets, Sreedhar Thirumala

LSU Doctoral Dissertations

Human adipose tissue provides a uniquely abundant and accessible source of adult stem cells. In response to chemical, hormonal or structural stimuli, these human adipose-derived adult stem (ASCs) cells can differentiate along multiple lineage pathways, including adipocytes, chondrocytes, myocytes, neurons and osteoblasts. Successful cryopreservation of scientifically and commercially important ASCs would revolutionize the tissue engineering and regenerative medicine industry. In this research, we have investigated the water transport phenomenon during freezing of several passages of adipose derived adult stem cells and generated the membrane permeability parameters in the presence and absence of cryoprotective agents. These measured permeability parameters were then …

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Revolutionizing Genomic Analyses: Mutation Analyses Using Novel Enzyme-Based Assays With Laser-Induced Fluorescence And Polymeric Microfluidic Devices As Electrophoretic Platforms, Rondedrick Deshaun Sinville Jan 2008

Revolutionizing Genomic Analyses: Mutation Analyses Using Novel Enzyme-Based Assays With Laser-Induced Fluorescence And Polymeric Microfluidic Devices As Electrophoretic Platforms, Rondedrick Deshaun Sinville

LSU Doctoral Dissertations

Polymer-based microelectrophoresis was investigated to analyze known (mutation detection) and unknown (mutation scanning) low-abundant mutations in genomic DNA with high diagnostic value for colorectal cancers. For our mutation detection assays, point mutations in the K-ras oncogene were identified using the ligase detection reaction (LDR). For the mutation scanning assay, which searches for sporadic mutations, an EndoV-LDR assay was utilized with mutations in the p53 tumor suppressor gene used as a model. A poly(methylmethacrylate), PMMA, microchip filled with a 4% linear polyacrylamide (LPA) gel was used to electrophoretically sort products formed from LDRs, which produced oligonucleotides <65 bp in length. Using microchip electrophoresis with the LPA, a 44 bp ligation product was resolved from a 100-fold molar excess of unligated primers (25 bp) in approximately 120 s, which was ~17 times faster than conventional capillary gel electrophoresis. In order to simplify the electrophoretic process and further reduce development time, the LDR products were sorted in the absence of the sieving gel using free solution conjugate electrophoresis (FSCE). FSCE incorporated polyamide “drag-tags” onto LDR primers, which provided DNA fragment mobilities in free solution that were dependent upon their size. LDR/drag-tagged (LDR-dt) products could be formed in a multiplexed format for mutant-to-wild-type ratios as low as 1 to 100 with single base resolution. Separations were conducted using capillary array electrophoresis (CAE) and PMMA microchips filled with only a TRIS buffer. Analysis times for the LDR-dt products were less than 11 min using CAE and ~85 s for PMMA microchips with high reproducible migration times within and between microchips. PMMA-based microchips were also evaluated for the identification of sporadic mutations using an endonuclease V – LDR (Endo V/LDR) technique. Endo V cleaves heteroduplexed DNA one base 3’ of single-base mismatched sites as well as nicking DNA at some matched sites as LDR reseals miscleaved sites to reduce false positive signals. Results suggested that Endo V/LDR products from p53 mutations could be successfully separated and detected using a PMMA microfluidic chip filled with a sparsely cross-linked replaceable polyacrylamide gel in less than 6 min, which was approximately 10-fold shorter compared to CAE.

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Chemotactic And Inflammatory Responses To And Recognition Of Encephalitozoon Spp. Of Microsporidia, Jeffrey Lynn Fischer Jan 2008

Chemotactic And Inflammatory Responses To And Recognition Of Encephalitozoon Spp. Of Microsporidia, Jeffrey Lynn Fischer

LSU Doctoral Dissertations

Microsporidiosis is an emerging disease among immunocompromised individuals who often present with chronic diarrhea. The intracellular, eukaryotic parasites responsible for this pathology can often disseminate, causing multiorgan infections. Dissemination of these pathogens is believed to occur through vehicular spread by macrophages. The macrophage response to microsporidia is poorly understood. The information, described herein, is focused on defining the host-pathogen interaction and subsequent inflammatory response in human monocyte-derived-macrophages (MDM) against Encephalitozoon spp. of microsporidia. Initial studies were designed to better define the infection kinetics in MDM using various microscopic analysis and novel staining approaches. Spore adherence and uptake occurs within the …

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Heterogeneous Catalyzed Macromolecular Hydrogenations In Oscillating Systems, Alan Bussard Jan 2008

Heterogeneous Catalyzed Macromolecular Hydrogenations In Oscillating Systems, Alan Bussard

LSU Doctoral Dissertations

An examination of novel oscillatory (alternating gas and liquid phase) reactors and heterogeneous catalysts for multi-phase macromolecular reactions was carried out. A monolith-containing square die with Pd/Al2O3 catalysts was used to successfully hydrogenate poly(styrene) (PS). The inherent pulse behavior of the extruder was found to be sufficient to approach intrinsic kinetics at low polymer concentrations. At higher (10 wt% PS) concentrations, forced pulsing was shown to have a greater impact on observed reaction rates. Selectivity with this extruder-fed reactor was better than with a stirred tank in all cases, due to a more plug-flow-like residence time distribution. While accurate control …

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Phylogenetics And New Approaches To Vaccine Development For West Nile, Arun Venkatesh Iyer Jan 2008

Phylogenetics And New Approaches To Vaccine Development For West Nile, Arun Venkatesh Iyer

LSU Doctoral Dissertations

West Nile virus (WNV) was first isolated in 1937 from a febrile Ugandan woman. WNV now has a worldwide distribution from Australia and India in the east to Europe and the United States in the west. The first human cases of WNV in the United States were detected in New York in 1999. The North American isolates were also found to be highly neuroinvasive and neurovirulent and in many cases leading to mortality or permanent CNS sequelae among humans. WNV was first isolated in Louisiana in 2001. The genome of this virus named LSU-AR01was sequenced and a detailed genetic analysis …

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Dactyls Reveal Evolutionary Patterns In Decapod Crustaceans, Jeffrey Guy Agnew Jan 2008

Dactyls Reveal Evolutionary Patterns In Decapod Crustaceans, Jeffrey Guy Agnew

LSU Doctoral Dissertations

Because of their high preservation potential and uses in foraging and defense, decapod crustacean dactyls (movable fingers of claws) are potentially excellent test subjects for an ongoing debate concerning the relative importance of top-down (predators) and bottom-up (prey) controls on morphologic diversification and evolution. The utility of dactyls for inferring evolutionary patterns were evaluated using living and subfossil xanthoid crabs sampled from the southeast U.S. Atlantic and Gulf of Mexico coasts, and were used to: (1) distinguish the roles of selection and constraint on dactyl morphology through allometric and shape comparisons in the context of the crab’s phylogenetic relationships and …

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Multimedia Mathematics Intervention For Math-Delayed Middle School Students, Lisa L. Stokes Jan 2008

Multimedia Mathematics Intervention For Math-Delayed Middle School Students, Lisa L. Stokes

LSU Doctoral Dissertations

The purpose of this study is to determine if the Sharpening Math Skills Lab technology-mediated mathematics instructional practices for math-delayed middle school students have positive effects on their mathematics achievement and spatial visualization ability and to gauge student engagement in learning, implementation of the principles of instructional design, and attitudes toward mathematics instruction. The results of a recent meta-analysis report a range of significantly positive to significantly negative effect sizes which establish a need for further evaluation of academic achievement utilizing technology-mediated mathematics programs at the middle school level (Slavin, Lake, & Groff, 2007). The literature (Moreno & Mayer, 2000) …

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Moderately-Rapid Assessment Of Alkaline Desiccation Environmental Systems, David Alan Bates Jan 2008

Moderately-Rapid Assessment Of Alkaline Desiccation Environmental Systems, David Alan Bates

LSU Doctoral Dissertations

A moderately-rapid assessment tool was developed to analyze the waterless desiccation compost toilet investigated in a rural Mexican setting. Over 100 social factors were identified along with the applicable technical factors that influenced the low acceptability of the toilets. A 4-point rating scale was developed to increase the ability and speed of analyzing both the social and technical data. The treatment process was an alkaline-desiccation process with mean pH values of 8.2 ± sd 1.1 and water content of 18.3% ± sd 9.9, which resulted in mean fecal coliform values of 15.0 MPN/g ± sd 31.8, drastically lower than the …

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Evaluation Of Management Practices And Remediation Techniques For Improving Water Quality In Agricultural Systems, Theophilus Kene Udeigwe Jan 2008

Evaluation Of Management Practices And Remediation Techniques For Improving Water Quality In Agricultural Systems, Theophilus Kene Udeigwe

LSU Doctoral Dissertations

Surface water quality impairment is often associated with agricultural activities. In this study, the effects of three sugarcane residue management techniques, namely burning (BR), shredding (SR), and retention (RR) of residues on: surface water quality, carbon export, and chemical composition of organic matter in the runoff sediments were characterized. Separate studies were conducted to evaluate predictive relationships for biochemical oxygen demand (BOD) in agricultural effluents, and the effectiveness of bauxite residues (red and brown muds) in reducing soluble nutrient/pollutant release from manure-impacted soils. All the selected water quality parameters were determined using EPA-approved analytical methods. The RR technique exported lower …

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Molecular Diversity And Coat Protein Expression Of Sweet Potato Leaf Curl Virus, Dina Lida Gutierrez Reynoso Jan 2008

Molecular Diversity And Coat Protein Expression Of Sweet Potato Leaf Curl Virus, Dina Lida Gutierrez Reynoso

LSU Doctoral Dissertations

Leaf curl virus diseases have been reported in sweetpotato throughout the world. One of the causal agents is Sweet potato leaf curl virus (SPLCV) which belongs to the genus Begomovirus (family Geminiviridae). In the United States, SPLCV has been found infecting an ornamental sweetpotato and several breeding lines but not in sweetpotatoes grown for commercial production. SPLCV does not cause symptoms on Beauregard, the predominant sweetpotato cultivar in the US, but it can reduce its yield. Since SPLCV could become an important constraint for sweetpotato production; diagnosis, identification, and characterization are essential steps to develop an effective management program. The …

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A Novel Automated Approach Of Multi-Modality Retinal Image Registration And Fusion, Hua Cao Jan 2008

A Novel Automated Approach Of Multi-Modality Retinal Image Registration And Fusion, Hua Cao

LSU Doctoral Dissertations

Biomedical image registration and fusion are usually scene dependent, and require intensive computational effort. A novel automated approach of feature-based control point detection and area-based registration and fusion of retinal images has been successfully designed and developed. The new algorithm, which is reliable and time-efficient, has an automatic adaptation from frame to frame with few tunable threshold parameters. The reference and the to-be-registered images are from two different modalities, i.e. angiogram grayscale images and fundus color images. The relative study of retinal images enhances the information on the fundus image by superimposing information contained in the angiogram image. Through the …

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Evaluation Of Three Methods For Teaching Intraverbals To Children With Language Delays, Nicole Trosclair-Lasserre Jan 2008

Evaluation Of Three Methods For Teaching Intraverbals To Children With Language Delays, Nicole Trosclair-Lasserre

LSU Doctoral Dissertations

Direct instruction is often necessary to develop language or expand language use in individuals with language delays. Previous research has begun to identify certain training conditions that result in more efficient use of instructional time devoted to language development. Specifically, incorporating mands into the instructional arrangement, increasing the quality of reinforcement delivered for interspersed tasks, and including instructive feedback stimuli into the consequences of learning trials have all demonstrated more efficient learning of targeted language skills. The purpose of the current investigation was to compare three methods for teaching intraverbals (i.e., conversation skills) to individuals with deficits in this area. …

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Wax Extraction And Characterization From Full-Fat And Defatted Rice Bran, Junghong Kim Jan 2008

Wax Extraction And Characterization From Full-Fat And Defatted Rice Bran, Junghong Kim

LSU Doctoral Dissertations

Rice bran oil (RBO) contains 3-4% waxes (rice bran wax, RBW), which are composed of wax esters (WE), hydrocarbons, and other minor constituents. Saponified rice bran wax esters (RBWE) generate fatty acids, long chain alcohols, and phytosterols. Phytosterols and long chain fatty alcohols (policosanol) are known to reduce serum cholesterol and inhibit hepatic cholesterol synthesis. The yields and RBWE contents of RBO and RBW extracted from full-fat RB (FFRB) and defatted RB (DFRB) were determined using 6 different conditions with Soxhlet and Microwave-assisted extraction (MAE). The compositions of WEs from RBO and RBW were also compared. RBW was obtained from …

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Species Boundaries, Biogeography, And Intra-Archipelago Genetic Variation Within The Emoia Samoensis Species Group In The Vanuatu Archipelago And Oceania, Alison Madeline Hamilton Jan 2008

Species Boundaries, Biogeography, And Intra-Archipelago Genetic Variation Within The Emoia Samoensis Species Group In The Vanuatu Archipelago And Oceania, Alison Madeline Hamilton

LSU Doctoral Dissertations

Speciation, geographic variation, and genetic differentiation are fundamental processes that generate diversity, and understanding these processes are major goals of evolutionary biology. Evolutionary phenomena may be more observable on islands as compared to continental landmasses as a result of small population sizes, unoccupied niches, and the relative simplicity of island systems and their populations: physical isolation, shorter (and often well documented) geologic time scale, reduced faunal diversity, and lack of outside faunal influence. Yet, despite their incredible diversity, Pacific island faunas have received little research attention relative to other tropical regions. Using molecular data from several species of scincid lizards …

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Degradation And Water Quality Dynamics Of Sugarcane Residue In South Louisiana, Jose Roberto Deras Jan 2008

Degradation And Water Quality Dynamics Of Sugarcane Residue In South Louisiana, Jose Roberto Deras

LSU Doctoral Dissertations

PURPOSE: This research was conducted to assess methods to manage the residue blanket to avoid open field burning in sugarcane. Experiments were conducted in the state of Louisiana to evaluate methods to asses and potentially reduce water quality issues. METHODS: Concentrations and frequencies of biological compost tea were sprayed on sugarcane combine-harvester residue blanket to increase degradation rate and reduce potential for runoff water to transport nutrients and sediments that may impair water bodies, while sustaining suitable sugarcane yield. Carbon dioxide evolution rate (mg CO2-C) and CO2 fluxes were measured in laboratory and in open field conditions; as an index …

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Redutive Dehalogenation Of Chlorinated Alkanes By Novel Bacteria At The Petroprocessor Of Louisiana Inc. Superfund Site, Jun Yan Jan 2008

Redutive Dehalogenation Of Chlorinated Alkanes By Novel Bacteria At The Petroprocessor Of Louisiana Inc. Superfund Site, Jun Yan

LSU Doctoral Dissertations

A reductively dehalogenating enrichment culture was established using chloroalkane-contaminated groundwater from the PetroProcessors of Louisiana, Inc. (PPI) Superfund site. Two novel, strictly anaerobic bacterial strains, designated as BL-DC-8 and BL-DC-9, were isolated from the enrichment culture. These strains represent the first bacteria known to anaerobically dehalogenate 1,2,3-trichloropropane, the degradation pathway of which was determined. Both strains could be cultured in completely defined basal medium and were also able to dehalogenate a variety of other vicinally chlorinated alkanes including 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane, 1,2-dichloroethane, and 1,2-dichloropropane via dichloroelimination reactions. Chlorinated alkanes containing only a single chlorine substituent (1-chloropropane, 2-chloropropane), chlorinated alkenes (tetrachloroethene, trichloroethene, …

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Phylogeogaphy Of Nothoprocta Tinamous And The The Phylogeny Of The Tinamidae, Thomas Valqui Jan 2008

Phylogeogaphy Of Nothoprocta Tinamous And The The Phylogeny Of The Tinamidae, Thomas Valqui

LSU Doctoral Dissertations

Understanding how diversity is attained and maintained is one of the central questions in biology. In the Neotropics most attention has centered on the Amazonian lowlands, despite the recognized importance of the role of the Andes in South American diversity. In this dissertation, I address the question by using Nothoprocta tinamous as a model case for examining diversification in the Andes. As a group, they are a manageable size, they exhibit near-restriction to the Andes, they show multi-species parapatric distributions, and they have presumed limited dispersal ability. These are all useful attributes for a system to study speciation and diversification. …

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Retrotransposon-Mediated Instability In The Human Genome, Shurjo Kumar Sen Jan 2008

Retrotransposon-Mediated Instability In The Human Genome, Shurjo Kumar Sen

LSU Doctoral Dissertations

LINE-1 (Long Interspersed Element-1/L1) and Alu are two active retrotransposon families in the human genome that have the potential to create genomic instability either during the insertion of new elements or through ectopic recombination. However, recent in vitro analyses have demonstrated that these elements also repair DNA double-strand breaks, hence contributing to the maintenance of genomic integrity. As such, the comprehensive role of mobile elements in either creating or mitigating instability in primate genomes remains unclear. The recent sequencing of the chimpanzee and rhesus macaque genomes uniquely facilitates the accurate resolution of this question, as three-way computational alignment of the …

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How To Remember Thee?: Problems Of Memorialization In English Writing, 1558-1625, Sean Flory Jan 2008

How To Remember Thee?: Problems Of Memorialization In English Writing, 1558-1625, Sean Flory

LSU Doctoral Dissertations

This dissertation focuses on the use of funeral commemoration in religious and political controversies in early modern England. By examining the rhetoric used in funeral sermons and elegies, I show that commemorative writers use figural interpretation of the Bible to legitimize praise by linking the deceased to characters from scripture. Figural interpretation places the dead into a framework of ecclesiastical history and creates Protestant saints used as exempla in political and religious debates. This dissertation examines funeral sermons, elegies, and other commemorative poems written between 1558 and 1625. Chapter one discusses the development of figural interpretation in Elizabethan funeral sermons. …

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Characterization And Comparison Of Cell Frequency, Growth, And Multipotential Differentiation Of Adult Mesenchymal Stromal Cells Derived From Equine Bone Marrow And Adipose Tissue, Martin Andreas Vidal Jan 2008

Characterization And Comparison Of Cell Frequency, Growth, And Multipotential Differentiation Of Adult Mesenchymal Stromal Cells Derived From Equine Bone Marrow And Adipose Tissue, Martin Andreas Vidal

LSU Doctoral Dissertations

Equine bone marrow-derived mesenchymal stromal cells (MSCs) and adipose tissue-derived stromal cells (ASCs) were compared for frequency within their respective tissues, cell doubling characteristics and differentiation multipotential in culture based on histochemical staining and compositional analysis. Equine MSCs and ASCs from young adult horses were harvested and isolated from sternal bone marrow and supragluteal subcutaneous adipose tissue, respectively, and grown up to passage 10 (P10) to determine cell doubling characteristics. Limit dilution assays were performed on primary and passaged (P2, P4) MSCs and ASCs to determine the frequency of colony forming units with a fibroblastic phenotype (CFU-F), and the frequency …

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Isolation And Characterization Of Two Siamese Phenotypic Modifiers And Their Role In Endoreplication And Trichome Development, Remmy Wekesa Kasili Jan 2008

Isolation And Characterization Of Two Siamese Phenotypic Modifiers And Their Role In Endoreplication And Trichome Development, Remmy Wekesa Kasili

LSU Doctoral Dissertations

Recessive mutations in the ENS1 gene of Arabidopsis thaliana result in reduced trichome branching without altering the ploidy level of trichomes. This implies that ENS1 regulates trichome branching in an endoreplication-independent manner. Mutations in ENS1 also enhance the multicellularity of sim mutant trichomes indicating that ENS1 plays a role in the regulation of the cell cycle during trichome cell differentiation. We have shown that ENS1 is required by the negative regulators of trichome branching for the trichomes to achieve their supernumerary branching. The interaction between ens1-1 and try-JC suggests that ENS1 is involved in both primary and secondary branching events …

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