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Uncovering A Macrophage Transcriptional Program By Integrating Evidence From Motif Scanning And Expression Dynamics, Stephen A. Ramsey, Sandy L. Klemm, Daniel E. Zak, Kathleen A. Kennedy, Vesteinn Thorsson, Bin Li, Mark Gilchrist, Elizabeth S. Gold, Carrie D. Johnson, Vladimir Litvak, Garnet Navarro, Jared C. Roach, Carrie M. Rosenberger, Alistair G. Rust, Natalya Yudkovsky, Alan Aderem, Ilya Shmulevich Jan 2008

Uncovering A Macrophage Transcriptional Program By Integrating Evidence From Motif Scanning And Expression Dynamics, Stephen A. Ramsey, Sandy L. Klemm, Daniel E. Zak, Kathleen A. Kennedy, Vesteinn Thorsson, Bin Li, Mark Gilchrist, Elizabeth S. Gold, Carrie D. Johnson, Vladimir Litvak, Garnet Navarro, Jared C. Roach, Carrie M. Rosenberger, Alistair G. Rust, Natalya Yudkovsky, Alan Aderem, Ilya Shmulevich

Faculty Publications

Macrophages are versatile immune cells that can detect a variety of pathogen-associated molecular patterns through their Toll-like receptors (TLRs). In response to microbial challenge, the TLR-stimulated macrophage undergoes an activation program controlled by a dynamically inducible transcriptional regulatory network. Mapping a complex mammalian transcriptional network poses significant challenges and requires the integration of multiple experimental data types. In this work, we inferred a transcriptional network underlying TLR-stimulated murine macrophage activation. Microarray-based expression profiling and transcription factor binding site motif scanning were used to infer a network of associations between transcription factor genes and clusters of co-expressed target genes. The time-lagged …

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A Concordance Of Bosoms Reconstructing Lewis's Monk, Frederick S. Frank Jan 2008

A Concordance Of Bosoms Reconstructing Lewis's Monk, Frederick S. Frank

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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"From This Time, I Shall Survey Myself In The Glass With A Sort Of Philosophical Pleasure" Newton And Narcissism In Sir Isaac Newton's Philosophy Explain 'D For The Use Of The Ladies, Deborah N. Armintor Jan 2008

"From This Time, I Shall Survey Myself In The Glass With A Sort Of Philosophical Pleasure" Newton And Narcissism In Sir Isaac Newton's Philosophy Explain 'D For The Use Of The Ladies, Deborah N. Armintor

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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Abraham Cowley's Amiability, Tom Mason Jan 2008

Abraham Cowley's Amiability, Tom Mason

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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Critical Voices Humor, Irony, And Passion In The Literary Critics Of The Long Eighteenth Century, Philip Smallwood Jan 2008

Critical Voices Humor, Irony, And Passion In The Literary Critics Of The Long Eighteenth Century, Philip Smallwood

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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The Epic Dryden Never Wrote, Cedric D. Reverand Ii Jan 2008

The Epic Dryden Never Wrote, Cedric D. Reverand Ii

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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Voice And Laughter In Johnson's Criticism, Philip Smallwood Jan 2008

Voice And Laughter In Johnson's Criticism, Philip Smallwood

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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Unnatural Conjugation In Tristram Shandy, Book Vii, Arnd Bohm Jan 2008

Unnatural Conjugation In Tristram Shandy, Book Vii, Arnd Bohm

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

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Strategies For Commercialization Of Cryopreserved Fish Semen, Terrence R. Tiersch Jan 2008

Strategies For Commercialization Of Cryopreserved Fish Semen, Terrence R. Tiersch

Faculty Publications

Initial success in sperm cryopreservation occurred at about the same time for aquatic species and livestock. However, in the 50 plus years since then cryopreserved sperm of livestock has grown into a billion-dollar global industry, while cryopreserved sperm of aquatic species remains a research activity with little commercial application despite work in more than 90 species and more than 200 published reports. Most research work has focused on large-bodied culture and sport fishes, such as salmon, trout, carp, and catfish, and mollusks such as commercially important oyster and abalone species. However, only a few studies have addressed sperm cryopreservation in …

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Berberine Improves Glucose Metabolism Through Induction Of Glycolysis, Jun Yin, Zhanguo Gao, Dong Liu, Zhijun Liu, Jianping Ye Jan 2008

Berberine Improves Glucose Metabolism Through Induction Of Glycolysis, Jun Yin, Zhanguo Gao, Dong Liu, Zhijun Liu, Jianping Ye

Faculty Publications

Berberine, a botanical alkaloid used to control blood glucose in type 2 diabetes in China, has recently been reported to activate AMPK. However, it is not clear how AMPK is activated by berberine. In this study, activity and action mechanism of berberine were investigated in vivo and in vitro. In dietary obese rats, berberine increased insulin sensitivity after 5-wk administration. Fasting insulin and HOMA-IR were decreased by 46 and 48%, respectively, in the rats. In cell lines including 3T3-L1 adipocytes, L6 myotubes, C 2C12 myotubes, and H4IIE hepatocytes, berberine was found to increase glucose consumption, 2-deoxyglucose uptake, and to a …

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Relative Economic Benefits Of Tactical Anthelmintic Treatment And Urea-Molasses Block Supplementation Of Boer Goats Raised Under Extensive Grazing Conditions At Onderstepoort, Pretoria, South Africa, A. F. Vatta, R. C. Krecek, R. A. Pearson, M. F. Smith, M. O. Stenson, E. F. Van Wijk, L. J.S. Harrison Jan 2008

Relative Economic Benefits Of Tactical Anthelmintic Treatment And Urea-Molasses Block Supplementation Of Boer Goats Raised Under Extensive Grazing Conditions At Onderstepoort, Pretoria, South Africa, A. F. Vatta, R. C. Krecek, R. A. Pearson, M. F. Smith, M. O. Stenson, E. F. Van Wijk, L. J.S. Harrison

Faculty Publications

The potential economic benefits of combining tactical anthelmintic treatment for gastrointestinal nematodes and nutritional supplementation with urea-molasses blocks were examined in Boer goats raised under extensive grazing conditions in the summer rainfall area of South Africa. Eight groups of nine goats were monitored over a 12-month period from 1 October 2002 to 9 October 2003. Ad libitum nutritional supplementation with urea-molasses blocks was provided when the goats were housed at night, during the summer (wet season-December 2002 to February 2003), and/or the winter (dry season-June 2003 to August 2003). All the goats were treated symptomatically for Haemonchus contortus infection when …

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Survey Of Forensically-Important Calliphoridae In Kingston And St. Andrew, Jamaica, West Indies, Wayne Anthony Cranston Jan 2008

Survey Of Forensically-Important Calliphoridae In Kingston And St. Andrew, Jamaica, West Indies, Wayne Anthony Cranston

LSU Master's Theses

The first research to be done in Jamaica on forensically-important species was conducted at the Government of Jamaica Forensic Laboratory in Kingston and St. Andrew in two phases. Phase 1was conducted from July 5 to July 24, 2007, and phase 2 was conducted from February 11 to February 28, 2008. In the phase 1 study, one local black colored Landrace pig (Sus scrofa L.) carcass that weighed 21kg was used as a model for human bodies to determine the rate of decomposition and the pattern of insect succession on decomposing bodies in Jamaica. Ants were the first arthropods to arrive …

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Dietary Resistant Starch Increases Hypothalamic Pomc Expression Independent Of Capsaicin-Sensitive Neurons In Rats, Li Shen Jan 2008

Dietary Resistant Starch Increases Hypothalamic Pomc Expression Independent Of Capsaicin-Sensitive Neurons In Rats, Li Shen

LSU Master's Theses

Resistant starch (RS) is fermentable dietary fiber. It has been shown that inclusion of resistant starch in the diet causes decreased body fat accumulation and altered gut hormone profile. Gut hormone has complex effect on neuropeptides’ expression in the brain hypothalamic area which is regarded as key factors in regulation of energy homeostasis. In this project, thereby, it is proposed that 1) the hypothalamic neuropeptide Y (NPY), agouti-related peptide (AgRP) and pro-opiomelanocortin (POMC) mRNA expression may be altered by RS feeding; 2) afferent vagal nerves might be involved in this process. Animal experiment was conducted to investigate the hypothesis. The …

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Modeling And Design Of Low Cost Customizable Household Robot, Bharat Narahari Jan 2008

Modeling And Design Of Low Cost Customizable Household Robot, Bharat Narahari

LSU Master's Theses

Just as the growth of Personal computer, Mobile phones and Automobiles took place in last 3 decades, the personal robotics industry still in its nascent stage, is heading in the same direction. This thesis explores the concept of customizable household robots (CHR) in the robotics community. An attempt has been made to design a customizable robot by extending the 2 wheel differential drive kinematic model to 4 wheel independent differential drive kinematic model. A framework for CHR is developed which will be able to do various household repetitive tasks. Just as we can assemble a PC by buying its individual …

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Influence Of Row Spacing And Debris Distribution On Vegetation And Small Mammals In Louisiana Pine Plantations, Anne M. Bechard Jan 2008

Influence Of Row Spacing And Debris Distribution On Vegetation And Small Mammals In Louisiana Pine Plantations, Anne M. Bechard

LSU Master's Theses

Site preparation after clearcuts directly affects surrounding small mammal populations. Differences in bed row spacing and arrangement of debris can impact structure and composition of vegetation communities, which influence small mammal habitat. We surveyed vegetation and small mammals in 2 different row spacings (14 ft and 20 ft) and 2 different debris distributions (piled and scattered) in 4 clearcuts owned by Weyerhaeuser in Louisiana during 2006-2007. Our objectives were to examine effects of row spacing and debris distribution on vegetation, to look at responses of small mammal densities to row spacing and debris distribution, and to see how small mammals …

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Effects Of Centrifugation On Equine Spermatozoa Immediately And After Cooling For 24 Hours, Jose Augusto Len Jan 2008

Effects Of Centrifugation On Equine Spermatozoa Immediately And After Cooling For 24 Hours, Jose Augusto Len

LSU Master's Theses

Len, Jose Augusto, MVZ, Universidad de Guadalajara, 1994 Master of Science, Summer Commencement, 2008 Major: Veterinary Medical Sciences Effects of Centrifugation on Equine Spermatozoa Immediately and After Cooling for 24 Hours Thesis directed by Professor Bruce Eilts Pages in thesis, 60. Words in abstract, 245. ABSTRACT The objectives of this study were to determine the effects of centrifugation on equine sperm progressive motility, plasma membrane integrity (viability), and acrosome integrity. We hypothesized that high centrifugation forces would be detrimental to equine sperm, yet recovery rates would increase. Ejaculates from six stallions were collected, extended (INRA96) to a concentration of 25 …

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Comorbid Psychological Disorder In Individuals With Intellectual Disabilities And Autism Spectrum Disorders, Jessica Ann Boisjoli Jan 2008

Comorbid Psychological Disorder In Individuals With Intellectual Disabilities And Autism Spectrum Disorders, Jessica Ann Boisjoli

LSU Master's Theses

Autism spectrum disorders (ASD) are characterized by deficits in three areas of functioning: communication, socialization, and restricted interests/repetitive behavior. With the rise in diagnoses of ASD in recent years, these disorders have received increasing recognition by researchers and clinicians. These efforts have largely been with young children. However, the study of ASD and comorbid disorders in adults with intellectual disability (ID) has been almost nonexistent. While there are measures available to assess comorbid disorders in people with only ID, the differences in adults with ASD and ID and adults with ID alone may also be a good deal different than …

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A Trinity Of Beliefs And A Unity Of The Sacred: Modern Vodou Practices In New Orleans, Elizabeth Thomas Crocker Jan 2008

A Trinity Of Beliefs And A Unity Of The Sacred: Modern Vodou Practices In New Orleans, Elizabeth Thomas Crocker

LSU Master's Theses

This thesis explores the ways in which Vodou is practiced in New Orleans today. Tourism has capitalized off the exotic appeal of Vodou but that does not rule out the actual practice of the religion in these public retail settings. Generations of New Orleanians have been raised in the religion and while their practices are often secret, Vodou lies beneath the surface of spaces and events going on in the city. Immigrants and converts that have been trained in Haitian Vodou have come into New Orleans, influencing and interacting with the spirituality of the Crescent City. These practices separate themselves …

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Characterizing The Epidemiology Of Bluetongue Virus Serotype One In South Louisiana, Michael Edward Becker Jan 2008

Characterizing The Epidemiology Of Bluetongue Virus Serotype One In South Louisiana, Michael Edward Becker

LSU Master's Theses

In November 2004, bluetongue virus (BTV) serotype 1 was detected for the first time in the U.S. from a hunter-killed deer in the marsh area of the Atchafalaya Delta in St. Mary Parish, LA. Subsequent serum surveys of three cattle farms further inland from the area where the deer was shot found bluetongue virus serotype 1 positive cattle on two of the three farms. The purpose of this study was to determine potential BTV vectors in the area where BTV-1 positive animals were detected and compare different trapping techniques for capturing specimens of the genus Culicoides. The study was conducted …

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The Influence Of Calf Selenium Status On Gpx-1 And 3 Activity And Liver Gpx-1 Mrna, Genevieve Elizabeth Tanner Jan 2008

The Influence Of Calf Selenium Status On Gpx-1 And 3 Activity And Liver Gpx-1 Mrna, Genevieve Elizabeth Tanner

LSU Master's Theses

The purpose of this research was to determine the influence of dietary Se on glutathione peroxidase (GPx)-1 and 3 activities and relative liver GPx mRNA levels in growing Holstein bull calves. Calves (n = 14) were started 28 d after birth on either a Se adequate (0.15 ppm Se) or deficient (0.01 ppm Se) diet consisting of 3 growth phases and maintained on the diet until 180 d of age. Blood samples were taken from each calf for determination of GPx-1 and GPx-3 activity. Three calves were euthanized at d 21 of age for determination of baseline liver GPx-1 mRNA …

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Effects Of Probiotics And Yeast Culture On Rumen Development And Growth Of Dairy Calves, Jennifer Marie Laborde Jan 2008

Effects Of Probiotics And Yeast Culture On Rumen Development And Growth Of Dairy Calves, Jennifer Marie Laborde

LSU Master's Theses

Forty-eight calves (heifers n=20, bulls n=28) were randomly assigned one of four dietary treatments which included no additive (C); the yeast culture Saccharomyces cerevisiae (YC); the probiotics Bacillus lichenformis and Bacillus subtilis (P); and both yeast culture and probiotics (YCP). Calves were administered treatments from day 2 to 56 in starter and from day 57 to 84 in grower diets. Body weights were measured at birth and weekly thereafter until day 112 of age. Wither and hip heights were measured weekly. Feed intake, water intake, and fecal scores were recorded twice daily until day 56. Rumen fluid was collected on …

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Hatchability Of Post-Peak Egg Production Broiler Breeder Eggs As Influenced By Pre-Incubation Warming, Cameron Benjamin Wiggins Ii Jan 2008

Hatchability Of Post-Peak Egg Production Broiler Breeder Eggs As Influenced By Pre-Incubation Warming, Cameron Benjamin Wiggins Ii

LSU Master's Theses

This research was conducted to determine the effects of pre-incubation warming on the hatchability of post-peak egg production broiler breeder eggs. An experiment with six trials was conducted with 7,920 freshly laid eggs from Ross 308 and 708 broiler breeders from 61-67-wks of age. For each trial, 1,320 eggs were used to determine if pre-incubation warming treatments of 0, 2, 4, and 6, or 0, 3, 6, and 9, or 0, 9, 12 and 15 hrs (at 37.6°C) could improve the hatchability of eggs stored (at 15.5°C) for three days. After a storage period of three days, the eggs were …

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Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee Jan 2008

Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee

LSU Master's Theses

Identification of synoviocytes surrounding the canine cranial cruciate ligament (CrCL) has not been investigated. Objectives 1) Develop and validate a technique to identify and quantify normal canine CrCL associated synoviocytes. 2) Compare synoviocyte phenotype proportions surrounding normal and abnormal canine CrCLs Design In vitro experimental Animals Cranial cruciate ligaments from 4 intact female and 6 intact adult male mixed-breed dogs (objective 1) and from 8 adult female hound dogs (objective 2) Methods Objective 1) Normal CrCLs - CD18 and HSP25 epitopes were colocalized using immunohistochemistry. Sagittal sections were quantified in the proximal, middle, and distal aspects of each section. Western …

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Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong Jan 2008

Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong

LSU Master's Theses

A conditional silencing system using anti-gB siRNAs was devised to investigate the structure and function of the KSHV gB. Transient co-transfection of plasmids constitutively expressing gB and anti-gB siRNAs in 293 cells substantially inhibited gB mRNA levels and protein production. Similarly, transient expression of siRNAs into the basal cavity-based lymphoma cells (BCBL-1) caused substantial reduction of gB transcription and protein synthesis. TaqMan real-time PCR and infectivity assays showed that gB was essential for virion egress and infectivity. Transfection of a codon-optimized gB not recognized by the anti-gB siRNAs, efficiently rescued virion egress and infectivity in BCBL-1 cells in the presence …

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Transcriptional Control Of The Adiponectin Gene By Stat5a, Joel Maier Jan 2008

Transcriptional Control Of The Adiponectin Gene By Stat5a, Joel Maier

LSU Master's Theses

Adiponectin is a hormone secreted from adipocytes that plays an important role in insulin sensitivity and fatty acid oxidation. The secretion of adiponectin from adipose tissue has been shown to be attenuated by treatment with prolactin (PRL). PRL is a potent STAT5 activator, and studies have indicated that it can modulate the expression of several genes in adipocytes. In this study, we demonstrate that 3T3-L1 adipocytes treated with PRL exhibit a reduction in adiponectin levels. Furthermore, we identified three putative STAT5 binding sites in the mouse adiponectin promoter and show that only one of these, located at -3809, binds nuclear …

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The Effects Of Quercetin On Skeletal Muscle Mitochondrial Biogenesis And Oxphos Regulation, Jeff Lee Soileau Jan 2008

The Effects Of Quercetin On Skeletal Muscle Mitochondrial Biogenesis And Oxphos Regulation, Jeff Lee Soileau

LSU Master's Theses

A high fat diet causes a reduction in the expression of the genes of the electron transport chain, mitochondrial carrier proteins, and PGC-1α and PGC-1ß mRNA. Mitochondrial dysfunction has been linked to numerous chronic diseases. The antioxidant quercetin has been shown to protect against damage caused by exogenous and endogenous free radicals. The purpose of this study was to determine if quercetin would protect the genes of mitochondrial biogenesis (PGC-1α and PGC-1ß) and OXPHOS regulation (NDUFB5 and SDHB) from the downregulation caused by consumption of a high fat diet. The quadriceps muscle was removed from 21 male, C57BL/6J mice that …

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Land Use And Water Quality Characterization Of Boeuf Basin, La, Joseph Wesley Leblanc Jan 2008

Land Use And Water Quality Characterization Of Boeuf Basin, La, Joseph Wesley Leblanc

LSU Master's Theses

The three primary goals of this project were to establish the ambient background of water quality in the Lake Boeuf basin in southeast Louisiana; establish land use patterns in the basin and the relation to water quality; and develop a preliminary plan to improve water quality through the use of best management practices and wetland assimilation. Urban and agricultural acreage borders the basin and runoff from these lands forces loadings of nutrients into the adjacent canals where they become channelized and ultimately exit the Boeuf Basin into Lac des Allemandes. From September 2007 until February 2008, water sampling occurred monthly …

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Postcolonial Writing In Louisiana: Surpassing The Role Of French Traditionalism In Alfred Mercier's L'Habitation Saint-Ybars, Mary Florence Cashell Jan 2008

Postcolonial Writing In Louisiana: Surpassing The Role Of French Traditionalism In Alfred Mercier's L'Habitation Saint-Ybars, Mary Florence Cashell

LSU Master's Theses

This thesis explores the roles of French patriarchal ideologies of the Enlightenment and exoticism in Alfred Mercier’s novel, L’habitation Saint-Ybars. His novel portrays the antebellum Creole plantation as a hierarchy of strict gender roles similar to those that Enlightenment philosophy espoused. I use the family in Bernardin de Saint-Pierre’s classic, Paul et Virginie, as one example of such a hierarchy. There are also, however, several instances where Mercier departs from the paternalistic norm. I interpret Mercier’s moving away from this model to be a declaration of a unique Louisianian identity.

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A Multi-Scale Assessment Of Den Selection Of Louisiana Black Bears (Ursus Americanus Luteolus) In Northern And Central Louisiana, Annelie Crook Crook Jan 2008

A Multi-Scale Assessment Of Den Selection Of Louisiana Black Bears (Ursus Americanus Luteolus) In Northern And Central Louisiana, Annelie Crook Crook

LSU Master's Theses

Understanding den selection for the Louisiana black bear (Ursus americanus luteolus) may provide insight into habitat requirements of the subspecies and assist in conservation and management efforts. With that goal, I assessed den selection of female Louisiana black bears at multiple spatial scales in northern and central Louisiana. I used 230 den-years to examine den type (tree or ground), microhabitat characteristics at dens, and effects of landscape characteristics on den selection. We also evaluated tree availability and reuse. Solitary and parturient females selected tree dens more frequently (65%) than ground dens. However, tree dens were not required for successful denning …

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Factors Potentially Influencing The Abundance Of Mosquitoes In Louisiana Rice Fields, Ana Maria Sanchez De Cuadra Jan 2008

Factors Potentially Influencing The Abundance Of Mosquitoes In Louisiana Rice Fields, Ana Maria Sanchez De Cuadra

LSU Master's Theses

The rice stink bug (Oebalus pugnax) is the major pest of late-season rice in Louisiana. Prior studies indicate that large populations of mosquitoes are often present in rice fields during the time that rice fields are infested with rice stink bugs. Lambda-cyhalothrin (Karate®) is widely used to control rice stink bugs. The purpose of this study was to determine if application of the insecticide Karate® at a rate of 0.033 kg/ha, for rice stink bug control had an effect on the population of mosquitoes in rice fields. This effect was assessed by monitoring natural populations of mosquitoes before and after …

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