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Development Of A Time-Dependent, Audio-Visual, Stated Choice Method Of Data Collection For Hurricane Evacuation Behavior, Ravindra Gudishala
Development Of A Time-Dependent, Audio-Visual, Stated Choice Method Of Data Collection For Hurricane Evacuation Behavior, Ravindra Gudishala
LSU Doctoral Dissertations
Revealed preference is the traditional method to collect hurricane evacuation behavior data. However, revealed preference surveys, as they are currently administered, have the disadvantage that they are unable to collect time-sensitive and policy-sensitive data needed to test evacuation policies. In contrast, data collected from a time-dependent, stated-choice survey will allow researchers to collect not only time-sensitive and policy-sensitive data but also information that will allow testing potential new evacuation policies. However, no research has been conducted to establish the methodology of such a survey. To fill the gap, this study was conducted to develop a new time-dependent, audio-visual, stated-choice method …
Interpretation And Expressive Imagery In The Butterfly Lovers Violin Concerto On Double Bass: Transcribed By Yung-Chiao Wei, Min-Tzu Chao
Interpretation And Expressive Imagery In The Butterfly Lovers Violin Concerto On Double Bass: Transcribed By Yung-Chiao Wei, Min-Tzu Chao
LSU Doctoral Dissertations
The purpose of this research paper is to show the interpretation of the Chinese piece—The Butterfly Lovers Violin Concerto—through expressing imagery. The concerto is composed by Chen Gang (陳鋼) and He Zhan-hao (何占豪) in 1959. In 2004, Yung-Chiao Wei, a double bass professor at Louisiana State University transcribed the piece for double bass. It is a very useful repertoire for double bass players to experience Chinese music through the plot’s expressing imagery. The Chinese musical language is different from Western music language in the concept of pentatonic scale, tone color, and tempo. I believe that double bass players can effectively …
Marketing Forest-Based Ecotourism In Sri Lanka: Predicting The Ecotourism Behavior And Defining The Market Segment Through A Behavioral Approach, Polwattage K. Perera
Marketing Forest-Based Ecotourism In Sri Lanka: Predicting The Ecotourism Behavior And Defining The Market Segment Through A Behavioral Approach, Polwattage K. Perera
LSU Doctoral Dissertations
Tourism industry is one of the main drivers of the global economy and plays a key role in regional development. As negative environmental and socio-cultural impacts of mass tourism became apparent, the appeal of alternative forms of tourism, especially ecotourism, continued to gain a broad recognition. As a result, ecotourism became one of the fastest growing sectors in global tourism during the past decade. Being a fast-developing country with ample natural resources, Sri Lanka can benefit from adopting and promoting ecotourism. At present, Sri Lanka’s ecotourism resources remain largely under-utilized. With rising demand, ecotourism operators are under pressure to meet …
Introduction And Establishment Of Cyrtobagous Salviniae Calder And Sands (Coleoptera: Curculionidae) For The Control Of Salvinia Minima Baker (Salviniaceae), And Interspecies Interactions Possibly Limiting Successful Control In Louisiana, Katherine A. Parys
LSU Doctoral Dissertations
Common salvinia, Salvinia minima Baker, is a floating aquatic invasive macrophyte that obstructs waterways and causes problems in wetlands across Louisiana and Texas. The salvinia weevil, Cyrtobagous salviniae Calder and Sands, has been released in over 14 countries around the world for the biological control of Salvinia spp. We successfully monitored the introduction and establishment of C. salviniae on S. minima in southern Louisiana between 2006 and 2010. Cyrtobagous salviniae significantly lowered the biomass of S. minima and increased the number of terminal buds damaged but had no significant impact on pH, dissolved oxygen, conductivity, surface temperature, percent of surface …
Biochemical And Genetic Insights Into Asukamycin Biosynthesis, Zhe Rui
Biochemical And Genetic Insights Into Asukamycin Biosynthesis, Zhe Rui
LSU Doctoral Dissertations
Asukamycin, a member of the manumycin family metabolites, is an antimicrobial and potential antitumor agent isolated from Streptomyces nodosus subsp. asukaensis. The entire asukamycin biosynthetic gene cluster was cloned, assembled and expressed heterologously in Streptomyces lividans. Bioinformatic analysis and mutagenesis studies elucidated the biosynthetic pathway at the genetic and biochemical level. Four gene sets, asuA-D, govern the formation and assembly of the asukamycin building blocks, a 3-amino-4-hydroxybenzoic acid (3,4-AHBA) core component, a cyclohexane ring, two triene polyketide chains and a 2-amino-3-hydroxycyclopent-2-enone (C5N) moiety to form the intermediate protoasukamycin. AsuE1 and AsuE2 catalyze the conversion of protoasukamycin to 4-hydroxyprotoasukamycin, which is …
Contingent Constellations: Frederick Douglass And The Fact Freedom, Tomohiro Hori
Contingent Constellations: Frederick Douglass And The Fact Freedom, Tomohiro Hori
LSU Doctoral Dissertations
Reading the celebrated Narrative (1845) of Frederick Douglass (1817-95) as well as his second autobiography My Bondage and My Freedom (1855) alongside the theories of freedom including Immanuel Kant’s and G. W. F. Hegel’s among others, this dissertation examines the process through which the young American slave Douglass discovers the idea of freedom and turns it into the primary object of his pursuit to the point that he stakes his life in his famed battle with the overseer Edward Covey. The experience of hearing other slaves’ voices—such as Aunt Hester’s cries and slave songs—opens his eyes to the darkest reality …
Pharmacological And Non-Pharmacological Approaches To Prevent Hypertension-Induced Renal Disease In The Spontaneously Hypertensive Rate, Carrie Marie Elks
Pharmacological And Non-Pharmacological Approaches To Prevent Hypertension-Induced Renal Disease In The Spontaneously Hypertensive Rate, Carrie Marie Elks
LSU Doctoral Dissertations
Hypertension affects 50 million Americans and remains the second leading cause of renal failure in the United States. Current pharmacological and non-pharmacological approaches to treat hypertension have proven effective, but the complexities of the disease and its renal effects warrant the need for new treatments. The hypothesis of this dissertation was that pharmacological or non-pharmacological approaches to reducing inflammation and oxidative stress would prevent hypertension-induced renal injury in the spontaneously hypertensive rat (SHR). In the first study, we blocked the inflammatory transcription factor, nuclear factor-kappa B (NF-ĸB), with pyrrolidine dithiocarbamate in the SHR kidney. In treated SHR, blood pressure decreased, …
Purification Of Fish Oils And Production Of Protein Powders With Epa And Dha Enriched Fish Oils, Huaixia Yin
Purification Of Fish Oils And Production Of Protein Powders With Epa And Dha Enriched Fish Oils, Huaixia Yin
LSU Doctoral Dissertations
Eicosapentaenoic (EPA) and docosahexaenoic (DHA) in marine oils are known to provide health benefits. However, marine oils are accompanied by saturated fatty acids. By enriching fish oils with EPA and DHA the beneficial effect of fish oils can be increased while limiting the detrimental effect of the saturated fatty acids. Fish oils need to be purified before consumption. A number of methods have been used for extracting and purifying EPA and DHA enriched fish oils; however, thermal degradation and oxidation of polyunsaturated fatty acids (PUFAs) often occurs as a result of exposure to high temperatures, solvents and adverse conditions during …
Aquatic Macroinvertebrate And Nekton Community Structure In A Chenier Marsh Ecosystem: Implications For Whooping Crane Prey Availability, Sung-Ryong Kang
Aquatic Macroinvertebrate And Nekton Community Structure In A Chenier Marsh Ecosystem: Implications For Whooping Crane Prey Availability, Sung-Ryong Kang
LSU Doctoral Dissertations
A suitable foraging habitat model based on prey density, biomass, and energy values in varying hydrologic conditions can provide an objective quantifiable method to assess habitat conditions for the Whooping Crane (Grus americana). Nekton and macroinvertebrates are considered the important prey sources in Whooping Crane's diet at different times. This study focuses on the understanding of habitat relationships of nekton and macroinvertebrate assemblage in the Chenier Plain to provide a foundation for the development for foraging suitability models for the reintroduced Whooping Crane. In this dissertation, in a laboratory study I experimentally examined the effect of salinity on …
Student Problem Solving Communication Processes While Completing Multimedia Case Studies: A Look Into The Relationship Among Levels Of Collaboration, Problem Solving Processes, And Problem Solving Performance On Individual And Group Levels, Jan Elizabeth Broussard
LSU Doctoral Dissertations
In an effort to better prepare students to become productive members of the work force, educators must grant students the opportunities to become critical thinkers. Students need to be able to learn to inquire, create, and think critically in order to obtain meaningful information from the abundance of knowledge that is accessible to them through the Internet and the World Wide Web. This quasi-experimental study examined the effects of online collaborative problem solving processes during a multimedia case study. Sixty-one students in two undergraduate management information systems classes agreed to participate. The experimental class, section 01, was required to use …
Mapping And Modeling Of Neglected Tropical Diseases In Brazil And Bolivia, Paula Mischler
Mapping And Modeling Of Neglected Tropical Diseases In Brazil And Bolivia, Paula Mischler
LSU Doctoral Dissertations
Accurately defining disease distributions and calculating disease risk is an important step in the control and prevention of diseases. This study used geographical information systems and remote sensing technologies within the MaxEnt ecological niche modeling program to create predictive risk maps for leprosy and Schistosomiasis in Brazil and Chagas disease in both Brazil and Bolivia. New disease cases were compiled for leprosy, Schistosomiasis, and Chagas disease from the Brazilian ministry of Health for 2001 to 2009 and the data was stratified to a 10,000 population for each municipality. Bolivian Chagas prevalence rates were calculated from 2007 to 2009 survey data. …
The Tick Response To Rickettsial Dissemination During Typical And Atypical Rickettsial Infection, Piyanate Sunyakumthorn
The Tick Response To Rickettsial Dissemination During Typical And Atypical Rickettsial Infection, Piyanate Sunyakumthorn
LSU Doctoral Dissertations
Ticks are the only disease vectors for spotted fever group (SFG) Rickettsia which are obligate intracellular bacteria belonging to the genus Rickettsia. In nature, ticks maintain the infection of SFG Rickettsia via vertical and horizontal transmission. However, the prevalence of rickettsial transmission is limited to certain species of ticks, and this limitation is known as a specific tick/Rickettsia relationship. Due to the continuous increase of tick-borne rickettsial disease cases in the United States, which contrasts with very low prevalence of Rickettsia in tick vectors, the study of vector competence of tick to Rickettsia is needed in order to understand the …
Calcium-Dependent Mechanisms In The Chicken Retina, Merve Tekmen
Calcium-Dependent Mechanisms In The Chicken Retina, Merve Tekmen
LSU Doctoral Dissertations
Multiple classes of retinal amacrine cells use L-type Ca2+ channels to mediate synaptic transmission. I have used whole cell voltage clamp recordings from primary cultures of amacrine cells to investigate the regulation of these channels. In this study, I show that inhibiting mitochondrial calcium uptake (MCU) caused a reversible reduction in the Ca2+ current amplitude. Replacing external Ca2+ with Ba2+ minimized the effects of blocking MCU indicating that the Ca2+ influx is the primary source of the inhibition. With 1,2-bis-(o-aminophenonxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA) in the recording pipette, MCU inhibition caused an increase in the current amplitude indicating that the fast buffering …
Proteomic Analysis Of The Blood Of Alligator Mississippiensis, Lancia Nadinia Fallen Darville-Bowleg
Proteomic Analysis Of The Blood Of Alligator Mississippiensis, Lancia Nadinia Fallen Darville-Bowleg
LSU Doctoral Dissertations
My research focus was to investigate alligator blood using mass spectrometry-based proteomics methods to understand their innate immune systems. The first goal was to sequence peptides and proteins from the blood serum and leukocytes using tandem mass spectrometry and de novo sequencing. The second goal was to determine the function of these biological molecules and their relationship to the immune system. One- and two-dimensional gel electrophoresis was used to separate proteins from alligator leukocytes, which were enzymatically digested. The peptides were measured using reversed phase nano-high performance liquid chromatography coupled with tandem mass spectrometry (nano-HPLC-MS/MS) followed with de novo sequencing. …
Spatial Ecology Of Adult Spotted Seatrout, Cynoscion Nebulosus, In Louisiana Coastal Waters, Jody Lynn Callihan
Spatial Ecology Of Adult Spotted Seatrout, Cynoscion Nebulosus, In Louisiana Coastal Waters, Jody Lynn Callihan
LSU Doctoral Dissertations
Spotted seatrout, Cynoscion nebulosus, are common in estuaries and coastal waters of the south Atlantic and Gulf of Mexico and are of considerable recreational and economic importance. Still, the spatial ecology of this species is under-studied and poorly resolved, especially in Louisiana waters. To address this important knowledge gap, I examined the movements, distribution, and habitat use of adult spotted seatrout in coastal Louisiana primarily using high-resolution acoustic telemetry and secondarily, conventional tagging (mark-recapture) data. At the largest spatial scale investigated, I found that adults exhibited a high degree of estuarine fidelity and rarely undertook large-scale movements in excess of …
Preferences Of Louisiana Agronomic Crop Producers And Crop Consultants Regarding Sources Of Information Related To Agricultural Production, Kenneth Craig Gautreaux
Preferences Of Louisiana Agronomic Crop Producers And Crop Consultants Regarding Sources Of Information Related To Agricultural Production, Kenneth Craig Gautreaux
LSU Doctoral Dissertations
The dissemination of information related to agronomic crop production is crucial to the success of the agriculture industry in Louisiana. This information is distributed by various sources and through multiple methods. The primary purpose of this study was to determine the preferences of Louisiana agronomic crop producers and crop consultants in regards to acquiring information related to their agricultural operations. A secondary purpose was to determine how agronomic crop producers perceived selected LSU AgCenter information sources on accuracy, awareness and frequency of use. Data for the study were obtained through surveys completed by 176 agronomic crop producers and 32 crop …
The Role Of Ad-36 And Its E4orf-1 Protein In Modulating Glycemic Control, Emily Jane Dhurandhar
The Role Of Ad-36 And Its E4orf-1 Protein In Modulating Glycemic Control, Emily Jane Dhurandhar
LSU Doctoral Dissertations
Current treatment strategies for Type 2 Diabetes Mellitus (T2DM) include a range of anti-diabetic drugs, supplemented by lifestyle modifications to reduce dietary fat intake and body fat. However, for their anti-diabetic action, most drugs recruit insulin signaling pathways, which are already impaired in T2DM. Also, compliance and success in achieving sustained improvements in diet or obesity over the long term is marginal. Therefore, an agent that improves diabetes independent of insulin signaling or lifestyle changes may be highly useful. Human adenovirus Ad36 offers such a model. Ad36 improves glycemic control in chow-fed mice or rats and attenuates diabetes and hepatic …
Structure-Function Relationships In Poly(3-Hexylthiophene)S Formed Via Manipulation Of Oxidative Coupling Routes, Warren James Solfiell
Structure-Function Relationships In Poly(3-Hexylthiophene)S Formed Via Manipulation Of Oxidative Coupling Routes, Warren James Solfiell
LSU Doctoral Dissertations
This investigation focused on the analytical characterization of poly(3-hexylthiophene), a heterocyclic conducting polymer system, with an overall objective of elucidating the effects that the experimental conditions during chemical oxidation using iron(III) chloride as an oxidant impart on the structure of the resulting polymers. Poly(3-alkylthiophene)s are extensively studied materials due to their high electrical conductivities and unique optical properties that make them extremely valuable for use in contemporary technological applications and devices. Specifically, the aim of this research was to determine the dependence of structural characteristics, such as endgroups, molecular weights, and regioregularities, on the solvent employed for the reaction and …
Stories Of The Proterozoic Earth: Paleoproterozoic Chuanlinggou Arcitarchs And Neoproterozoic ¹⁷O-Depleted Barite, Yongbo Peng
Stories Of The Proterozoic Earth: Paleoproterozoic Chuanlinggou Arcitarchs And Neoproterozoic ¹⁷O-Depleted Barite, Yongbo Peng
LSU Doctoral Dissertations
This dissertation consists of two independent topics, both critical to understanding Earth history during the Proterozoic Eon. (1) Paleoproterozoic Chuanlinggou Acritarchs: The eukaryotic affinity of acritarchs from the Chuanlinggou Formation has been questioned because of lack of indisputable morphological evidence. In this study, Chuanlinggou acritarchs were examined by jointly using optical microscopy, scanning electron microscopy, and transmission electron microscopy. In most cases, an ovoidal group of acritarchs represents a half-vesicle following a complete longitudinal rupture, which is a morphological model different from a whole envelope with medial splits, as proposed by earlier studies. This ovoidal group displays a bipolar morphology, …
Transnational Spaces And Communal Land Tenure In A Caribbean Place: "Barbuda Is For Barbudans", Amy E. Potter
Transnational Spaces And Communal Land Tenure In A Caribbean Place: "Barbuda Is For Barbudans", Amy E. Potter
LSU Doctoral Dissertations
In the last decade, transnational migration research has gained considerable ground in geography. There is still more to be done, however, in order to understand the complex relationship between migrants and the lands they leave behind. The island of Barbuda in the Lesser Antilles is the ideal place to study the larger issues of transnational migration on a smaller scale, particularly research that focuses on both migration and land tenure. Barbudan land tenure is common property, something that was in practice for more than a century and formalized into law in January of 2008. Because of this particular system of …
Decoupling User Interface Design Using Libraries Of Reusable Components, Rajesh Sankaran
Decoupling User Interface Design Using Libraries Of Reusable Components, Rajesh Sankaran
LSU Doctoral Dissertations
The integration of electronic and mechanical hardware, software and interaction design presents a challenging design space for researchers developing physical user interfaces and interactive artifacts. Currently in the academic research community, physical user interfaces and interactive artifacts are predominantly designed and prototyped either as one-off instances from the ground up, or using functionally rich hardware toolkits and prototyping systems. During this prototyping phase, undertaking an integral design of the interface or interactive artifact’s electronic hardware is frequently constraining due to the tight couplings between the different design realms and the typical need for iterations as the design matures. Several current …
Influences Of Landscape Characteristics On The Nesting Ecology Of Female Wild Turkeys And Behavior Of Raccoons, Michael E. Byrne
Influences Of Landscape Characteristics On The Nesting Ecology Of Female Wild Turkeys And Behavior Of Raccoons, Michael E. Byrne
LSU Doctoral Dissertations
Nest predation is the principle source of reproductive failure in many bird species. Understanding nest predation requires knowledge of interactions between landscape characteristics, and the ecology and behavior of birds and local nest predators. I studied nesting ecology and multi-scale habitat selection of female wild turkeys and the habitat selection and searching behaviors of raccoons, an important nest predator, in a bottomland hardwood forest in Louisiana. My objective was to evaluate the relationships between habitat, wild turkey nest site selection, and raccoon foraging behavior. I used first-passage time (FPT) analysis on nightly foraging tracks of raccoons during the turkey nesting …
Rights, Needs, And The Creation Of Ethical Community, Natalie Susan Gaines
Rights, Needs, And The Creation Of Ethical Community, Natalie Susan Gaines
LSU Doctoral Dissertations
Despite a half-century of dominance by the rights discourse, the supremacy of rights in theories of human obligation has recently come under attack from political theorists. Though scholars have questioned the ability of rights to explain satisfactorily the responsibility we have for the well-being of others, there are few viable alternatives offered. In this dissertation, I argue that a theory of needs provides a better explanation of the intellectual and moral foundations of obligation. Human need is deeply rooted in subjective potentiality, but studies in human psychology have also provided an increasingly universal picture of the needs human beings experience. …
Characterization Of Novel Akermanite:Poly-E-Caprolactone Scaffolds For Bone Tissue Engineering Aapplications Combined With Human Adipose-Derived Stem Cells, Andre S. Zanetti
Characterization Of Novel Akermanite:Poly-E-Caprolactone Scaffolds For Bone Tissue Engineering Aapplications Combined With Human Adipose-Derived Stem Cells, Andre S. Zanetti
LSU Doctoral Dissertations
The development of porous materials useful as scaffolds for the sustained three-dimensional (3D) growth of human adipose-derived stem cells (hASC) is of particular interest to facilitate healing after musculoskeletal injuries. In this study, a composite porous material obtained by blending akermanite with poly-e-caprolactone (PCL) is proposed as novel alternative to bone tissue regeneration. The objectives of this study are (1) to characterize the akermanite:PCL scaffold properties; (2) to investigate the in vitro osteogenic potential of hASC loaded to optimal akermanite:PCL scaffolds; (3) to assess the metabolic activity and osteogenesis of hASC loaded to optimal akermanite:PCL scaffolds post-thawing using optimal cryopreservation …
Development And Application Of Serogroup-Independent And Serogroup-Specific Loop-Mediated Isothermal Amplification Assays For Detecting Shiga Toxin-Producing Escherichia Coli, Fei Wang
LSU Doctoral Dissertations
Shiga toxin-producing Escherichia coli (STEC), encompassing E. coli O157:H7 and non-O157 STEC, is a significant cause of foodborne illnesses and deaths in the United States and worldwide. Shiga toxins (encoded by stx) and intimin (encoded by eae) are important virulence factors for STEC strains causing infection. Although E. coli O157:H7 remains to be the single most common STEC causing disease, the clinical importance of non-O157 STEC is on the rise worldwide. And six major serogroups (O26, O45, O103, O111, O121, and O145) accounted for over 70% of non-O157 STEC infections in the United States. Loop-mediated isothermal amplification (LAMP) is a …
Arginine Metabolism In The Edwardsiella Ictaluri- Channel Catfish Macrophage Dynamic, Wes Arend Baumgartner
Arginine Metabolism In The Edwardsiella Ictaluri- Channel Catfish Macrophage Dynamic, Wes Arend Baumgartner
LSU Doctoral Dissertations
Edwardsiella ictaluri encodes a urease operon and an arginine decarboxylase (AdiA) that are required for virulence in head kidney derived macrophages (HKDM). The urease produces ammonia in amounts sufficient to alter environmental pH from acid to neutral. A hypothetical model was proposed, involving arginine metabolism in E. ictaluri infected HKDM, focusing on bacterial urease, AdiA, a second arginine decarboxylase (SpeA), and agmatinase (SpeB). Using fluorescence based ratiometric pH determination of E. ictaluri in live HKDM, it was shown that E. ictaluri modulates HKDM phagosome pH to above six. Urease and AdiA mutants failed to up-regulate vacuole pH, while vacuole pH …
Crisis Management In Organizations: An Exploratory Study Of Factors That Affect Strategy Formation And Selection, Tulika Sinha
Crisis Management In Organizations: An Exploratory Study Of Factors That Affect Strategy Formation And Selection, Tulika Sinha
LSU Doctoral Dissertations
ABSTRACT This study investigated factors that influence the strategic decision-making process, specifically, strategy formation and selection during a crisis. It accomplished this by integrating theoretical concepts from both strategic management and crisis communication literature. Key organizational, environmental, and management factors--comprehensiveness, formalization, uncertainty, politicization, external corporate environment, crisis responsibility, impact of the crisis, stakeholder interests, and top management characteristics --were tested for their role in the strategy formation and selection process using regression analysis. This study used both quantitative and qualitative research methods. The quantitative aspect of the research involved conducting online surveys of senior management within the chemical industry and …
Formation And Stabilization Of Combustion-Generated Environmentally Persistent Free Radicals On Transition Metal Oxides Supported On Silica, Eric Warren Pimentel Vejerano
Formation And Stabilization Of Combustion-Generated Environmentally Persistent Free Radicals On Transition Metal Oxides Supported On Silica, Eric Warren Pimentel Vejerano
LSU Doctoral Dissertations
The formation of environmentally persistent free radicals in combustion system was investigated from chemisorptions of chlorine- and hydroxy-substituted benzenes on transition metal oxide surface under post-combustion conditions. This manuscript reports the formation of EPFRs on silica particles containing 5% Fe(III)2O3, Ni(II)O, and Zn(II)O. The EPFRs are formed by the chemisorption of substituted aromatic molecular adsorbates on the metal cation center followed by electron transfer from the adsorbate to the metal ion at temperatures from 150 to 400 oC. Depending on the nature of the adsorbate and the temperature, two organic EPFRs were formed: a phenoxyl-type radical, which has a lower …
Quantitative Mapping Of Cyanobacterial Blooms Using Oceansat-1 Ocm Satellite Data, Padmanava Dash
Quantitative Mapping Of Cyanobacterial Blooms Using Oceansat-1 Ocm Satellite Data, Padmanava Dash
LSU Doctoral Dissertations
Cyanobacteria represent a major harmful algal group in fresh to brackish water environments. Lac des Allemands, a freshwater lake of 49 km2 southwest of New Orleans, Louisiana, provides a natural laboratory for remote characterization of cyanobacteria blooms because of their seasonal occurrence. This dissertation makes a contribution to research methodology pertaining to atmospheric correction of satellite data and development of remote sensing algorithms to quantify cyanobacterial pigments. The Ocean Color Monitor (OCM) sensor provides radiance measurements similar to Sea-viewing Wide Field-of-View Sensor (SeaWiFS) but with higher spatial resolution. However, OCM does not have a standard atmospheric correction procedure and …
Inhibition And Nucleic Acid Binding Studies Of The Carboxyltransferase Component Of Bacterial Acetyl-Coa Carboxylase, Brian Benson
Inhibition And Nucleic Acid Binding Studies Of The Carboxyltransferase Component Of Bacterial Acetyl-Coa Carboxylase, Brian Benson
LSU Doctoral Dissertations
Acetyl-CoA carboxylase is an essential enzyme, as it catalyzes the first committed and regulated step in fatty-acid biosynthesis in all organisms excepting few Archaea and Eubacteria. Acetyl-CoA carboxylase from gram-negative and gram-positive bacteria is a multifunctional enzyme composed of three separate proteins. The carboxyltransferase subunit catalyzes the transfer of a carboxyl group from carboxybiotin to acetyl-CoA, forming malonyl-CoA. The crystal structure of the Escherichia coli (E. coli) carboxyltransferase component of acetyl-CoA carboxylase revealed a unique Zn-domain, presumed to mediate nucleic acid binding, that is absent in the eukaryotic enzyme. Notably, the Zn-domain, adjacent to the active site of carboxyltransferase, makes …