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Phthalocyanine-Peptide Conjugates For Epidermal Growth Factor Receptor Targeting, Benson G. Ongarora, Krystal R. Fontenot, Xiaoke Hu, Inder Sehgal, Seetharama D. Satyanarayana-Jois, Dm Graça H. Vicente Apr 2012

Phthalocyanine-Peptide Conjugates For Epidermal Growth Factor Receptor Targeting, Benson G. Ongarora, Krystal R. Fontenot, Xiaoke Hu, Inder Sehgal, Seetharama D. Satyanarayana-Jois, Dm Graça H. Vicente

Faculty Publications

Four phthalocyanine (Pc)-peptide conjugates designed to target the epidermal growth factor receptor (EGFR) were synthesized and evaluated in vitro using four cell lines: human carcinoma A431 and HEp2, human colorectal HT-29, and kidney Vero (negative control) cells. Two peptide ligands for EGFR were investigated: EGFR-L1 and -L2, bearing 6 and 13 amino acid residues, respectively. The peptides and Pc-conjugates were shown to bind to EGFR using both theoretical (Autodock) and experimental (SPR) investigations. The Pc-EGFR-L1 conjugates 5a and 5b efficiently targeted EGFR and were internalized, in part due to their cationic charge, whereas the uncharged Pc-EGFR-L2 conjugates 4b and 6a …

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Sustainability: More About The Toolmaker Than The Tools, R. Eugene Turner Apr 2012

Sustainability: More About The Toolmaker Than The Tools, R. Eugene Turner

Faculty Publications

A sustainable system is not necessarily a high-quality one, but it could be. We could, for example, “survive” on the desperate edge, as the remnants in a self-fouled and deteriorating environment. Why won’t a future sustainable system be just another industrial model of mass efficiency and throughput? Perhaps the incompatible outcomes are a choice between the sometimes nearly invisible civilizing aspects of culture nurturing respect, equality, and cooperation on one hand, and the greed and self-indulgences undermining social tolerance, empathy, and cooperation that ends up promoting violence and dehumanization. The human heritage is subtle, indestructible, and worth nurturing if we …

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High Dispersal Ability Inhibits Speciation In A Continental Radiation Of Passerine Birds, Santiago Claramunt, Elizabeth P. Derryberry, Jr V. Remsen, Robb T. Brumfield Apr 2012

High Dispersal Ability Inhibits Speciation In A Continental Radiation Of Passerine Birds, Santiago Claramunt, Elizabeth P. Derryberry, Jr V. Remsen, Robb T. Brumfield

Faculty Publications

Dispersal can stimulate speciation byfacilitating geographical expansion across barriers or inhibit speciation by maintaining gene flow among populations. Therefore, the relationship between dispersal ability and speciation rates can be positive or negative. Furthermore, an 'intermediate dispersal' model that combines positive and negative effects predicts a unimodal relationship between dispersal and diversification. Because both dispersal ability and speciation rates are difficult to quantify, empirical evidence for the relationship between dispersal and diversification remains scarce. Using a surrogate for flight performance and a species-level DNA-based phylogeny of a large South American bird radiation (the Furnariidae), we found that lineages with higher dispersal …

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Salinity- And Population-Dependent Genome Regulatory Response During Osmotic Acclimation In The Killifish (Fundulus Heteroclitus) Gill, Andrew Whitehead, Jennifer L. Roach, Shujun Zhang, Fernando Galvez Apr 2012

Salinity- And Population-Dependent Genome Regulatory Response During Osmotic Acclimation In The Killifish (Fundulus Heteroclitus) Gill, Andrew Whitehead, Jennifer L. Roach, Shujun Zhang, Fernando Galvez

Faculty Publications

The killifish Fundulus heteroclitus is abundant in osmotically dynamic estuaries and it can quickly adjust to extremes in environmental salinity. We performed a comparative osmotic challenge experiment to track the transcriptomic and physiological responses to two salinities throughout a time course of acclimation, and to explore the genome regulatory mechanisms that enable extreme osmotic acclimation. One southern and one northern coastal population, known to differ in their tolerance to hypo-osmotic exposure, were used as our comparative model. Both populations could maintain osmotic homeostasis when transferred from 32 to 0.4 p.p.t., but diverged in their compensatory abilities when challenged down to …

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Functional Hierarchy Of Herpes Simplex Virus 1 Viral Glycoproteins In Cytoplasmic Virion Envelopment And Egress, Dmitry V. Chouljenko, In Joong Kim, Vladimir N. Chouljenko, Ramesh Subramanian, Jason D. Walker, Konstantin G. Kousoulas Apr 2012

Functional Hierarchy Of Herpes Simplex Virus 1 Viral Glycoproteins In Cytoplasmic Virion Envelopment And Egress, Dmitry V. Chouljenko, In Joong Kim, Vladimir N. Chouljenko, Ramesh Subramanian, Jason D. Walker, Konstantin G. Kousoulas

Faculty Publications

Herpes simplex virus 1 (HSV-1) viral glycoproteins gD (carboxyl terminus), gE, gK, and gM, the membrane protein UL20, and membrane-associated protein UL11 play important roles in cytoplasmic virion envelopment and egress from infected cells. We showed previously that a recombinant virus carrying a deletion of the carboxyl-terminal 29 amino acids of gD (gDΔct) and the entire gE gene (ΔgE) did not exhibit substantial defects in cytoplasmic virion envelopment and egress (H. C. Lee et al., J. Virol. 83:6115-6124, 2009). The recombinant virus ΔgM2, engineered not to express gM, produced a 3-to 4-fold decrease in viral titers and a 50% reduction …

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I Was A Saint A Translation Of Je Fus Un Saint By Henri Vincenot, Kimberly Johnson Apr 2012

I Was A Saint A Translation Of Je Fus Un Saint By Henri Vincenot, Kimberly Johnson

Honors Capstones

No abstract provided.

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Synthesis And Cellular Investigations Of The 131-Lysyl Derivative Of Chlorin E6, Stewart Wynn Humble Apr 2012

Synthesis And Cellular Investigations Of The 131-Lysyl Derivative Of Chlorin E6, Stewart Wynn Humble

Honors Capstones

No abstract provided.

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Understanding The Auto-Regulation Of Leukotriene Biosynthesis A Model Of Inactivation For 5-Lipoxgenase, Erin E. Schexnaydre Apr 2012

Understanding The Auto-Regulation Of Leukotriene Biosynthesis A Model Of Inactivation For 5-Lipoxgenase, Erin E. Schexnaydre

Honors Capstones

No abstract provided.

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Phylogeny And Phylogeography Of Mantophryne (Anura: Microhylidae) Reveals Cryptic Diversity In New Guinea, Lauren Oliver Apr 2012

Phylogeny And Phylogeography Of Mantophryne (Anura: Microhylidae) Reveals Cryptic Diversity In New Guinea, Lauren Oliver

Honors Capstones

No abstract provided.

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Louisiana Agriculture Spring, 2012 Apr 2012

Louisiana Agriculture Spring, 2012

Louisiana Agriculture

No abstract provided.

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Angiogenic Deficiency And Adipose Tissue Dysfunction Are Associated With Macrophage Malfunction In Sirt1 -/- Mice, Fen Xu, David Burk, Zhanguo Gao, Jun Yin, Xia Zhang, Jianping Weng, Jianping Ye Apr 2012

Angiogenic Deficiency And Adipose Tissue Dysfunction Are Associated With Macrophage Malfunction In Sirt1 -/- Mice, Fen Xu, David Burk, Zhanguo Gao, Jun Yin, Xia Zhang, Jianping Weng, Jianping Ye

Faculty Publications

The histone deacetylase sirtuin 1 (SIRT1) inhibits adipocyte differentiation and suppresses inflammation by targeting the transcription factors peroxisome proliferator-activated receptor γ and nuclear factor κB. Although this suggests that adiposity and inflammation should be enhanced when SIRT1 activity is inactivated in the body, this hypothesis has not been tested in SIRT1 null (SIRT1 -/-) mice. In this study, we addressed this issue by investigating the adipose tissue in SIRT1 -/-mice. Compared with their wild-type littermates, SIRT1 null mice exhibited a significant reduction in body weight. In adipose tissue, the average size of adipocytes was smaller, the content of extracellular matrix …

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Plant Science In Forest Canopies - The First 30 Years Of Advances And Challenges (1980-2010), Margaret D. Lowman, Timothy D. Schowalter Apr 2012

Plant Science In Forest Canopies - The First 30 Years Of Advances And Challenges (1980-2010), Margaret D. Lowman, Timothy D. Schowalter

Faculty Publications

Contents: Summary 12 I. Introduction 12 II. History of canopy access 13 III. Consequences of whole-tree approaches to forest science 15 IV. Canopy communities - their inhabitants, environment, and processes 16 V. The role of forest canopies in providing ecosystem services 21 VI. Using canopy science as a 'hook' to inspire forest conservation 21 VII. Conclusions -'black boxes' in canopy science that remain 22 Acknowledgements 23 References 24 Summary: As an emerging subdiscipline of forest biology, canopy science has undergone a transition from observational, 'oh-wow' exploration to a more hypothesis-driven, experimental arena for rigorous field biology. Although efforts to explore …

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Phylogenetic Relationships In Sarraceniaceae Based On Conserved Sequences Generated From Sarracenia Alata, Danielle Fuselier Apr 2012

Phylogenetic Relationships In Sarraceniaceae Based On Conserved Sequences Generated From Sarracenia Alata, Danielle Fuselier

Honors Capstones

No abstract provided.

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Phosphorylation Studies Of Serine 663 On Human 5-Lipoxygenase, Pamela Arden Doerner Apr 2012

Phosphorylation Studies Of Serine 663 On Human 5-Lipoxygenase, Pamela Arden Doerner

Honors Capstones

No abstract provided.

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Ultraconserved Elements Are Novel Phylogenomic Markers That Resolve Placental Mammal Phylogeny When Combined With Species-Tree Analysis, John E. Mccormack, Brant C. Faircloth, Nicholas G. Crawford, Patricia Adair Gowaty, Robb T. Brumfield, Travis C. Glenn Apr 2012

Ultraconserved Elements Are Novel Phylogenomic Markers That Resolve Placental Mammal Phylogeny When Combined With Species-Tree Analysis, John E. Mccormack, Brant C. Faircloth, Nicholas G. Crawford, Patricia Adair Gowaty, Robb T. Brumfield, Travis C. Glenn

Faculty Publications

Phylogenomics offers the potential to fully resolve the Tree of Life, but increasing genomic coverage also reveals conflicting evolutionary histories among genes, demanding new analytical strategies for elucidating a single history of life. Here, we outline a phylogenomic approach using a novel class of phylogenetic markers derived from ultraconserved elements and flanking DNA. Using species-tree analysis that accounts for discord among hundreds of independent loci, we show that this class of marker is useful for recovering deep-level phylogeny in placental mammals. In broad outline, our phylogeny agrees with recent phylogenomic studies of mammals, including several formerly controversial relationships. Our results …

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Genomic Insights Into Adaptation To High-Altitude Environments, Z. A. Cheviron, R. T. Brumfield Apr 2012

Genomic Insights Into Adaptation To High-Altitude Environments, Z. A. Cheviron, R. T. Brumfield

Faculty Publications

Elucidating the molecular genetic basis of adaptive traits is a central goal of evolutionary genetics. The cold, hypoxic conditions of high-altitude habitats impose severe metabolic demands on endothermic vertebrates, and understanding how high-altitude endotherms cope with the combined effects of hypoxia and cold can provide important insights into the process of adaptive evolution. The physiological responses to high-altitude stress have been the subject of over a century of research, and recent advances in genomic technologies have opened up exciting opportunities to explore the molecular genetic basis of adaptive physiological traits. Here, we review recent literature on the use of genomic …

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Improvement Of Performance Of Cardiac Spect Camera Using Curved Detectors With Pinholes, Joyoni Dey Apr 2012

Improvement Of Performance Of Cardiac Spect Camera Using Curved Detectors With Pinholes, Joyoni Dey

Faculty Publications

SPECT is primarily used in the clinic for cardiac myocardial perfusion imaging. However, for SPECT, sensitivity is impaired due to the need for collimation. System resolution FWHM is poor as well (∼ 1 cm). In this work the resolution of a curved detector was theoretically derived. The advantage of a curved detector over a flat detector with pinhole collimation was demonstrated for cardiac applications using theoretical derivations as well as a ray-tracing voxel-based forward projector. For the flat detector using parameters close to what was expected for the new multi-pinhole GE Discovery system, it is shown that using a paraboloid …

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An Alu-Based Phylogeny Of Gibbons (Hylobatidae), James Michael Oldenburg Mar 2012

An Alu-Based Phylogeny Of Gibbons (Hylobatidae), James Michael Oldenburg

Honors Capstones

No abstract provided.

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Reply To Jenkins Et Al.: Evidence For Contaminating Oil Exposure Is Closely Linked In Space And Time To Biological Effects, Andrew Whitehead, Benjamin Dubansky, Charlotte Bodinier, Tzintzuni I. Garcia, Scott Miles, Chet Pilley, Vandana Raghunathan, Jennifer L. Roach, Nan Walker, Ronald B. Walter, Charles D. Rice, Fernando Galvez Mar 2012

Reply To Jenkins Et Al.: Evidence For Contaminating Oil Exposure Is Closely Linked In Space And Time To Biological Effects, Andrew Whitehead, Benjamin Dubansky, Charlotte Bodinier, Tzintzuni I. Garcia, Scott Miles, Chet Pilley, Vandana Raghunathan, Jennifer L. Roach, Nan Walker, Ronald B. Walter, Charles D. Rice, Fernando Galvez

Faculty Publications

No abstract provided.

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Faculty Senate Resolution 12-03, Louisiana State University And Agricultural & Mechanical College Mar 2012

Faculty Senate Resolution 12-03, Louisiana State University And Agricultural & Mechanical College

LSU Faculty Senate Publications

Faculty Senate Resolution on including class attendance among course grading criteria, adopted on March 14, 2012.

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Buffalo, Bush Meat, And The Zoonotic Threat Of Brucellosis In Botswana, Kathleen Anne Alexander, Jason Kenna Blackburn, Mark Eric Vandewalle, Risa Pesapane, Eddie Kekgonne Baipoledi, Phil H. Elzer Mar 2012

Buffalo, Bush Meat, And The Zoonotic Threat Of Brucellosis In Botswana, Kathleen Anne Alexander, Jason Kenna Blackburn, Mark Eric Vandewalle, Risa Pesapane, Eddie Kekgonne Baipoledi, Phil H. Elzer

Faculty Publications

Background: Brucellosis is a zoonotic disease of global importance infecting humans, domestic animals, and wildlife. Little is known about the epidemiology and persistence of brucellosis in wildlife in Southern Africa, particularly in Botswana. Methods: Archived wildlife samples from Botswana (1995-2000) were screened with the Rose Bengal Test (RBT) and fluorescence polarization assay (FPA) and included the African buffalo (247), bushbuck (1), eland (5), elephant (25), gemsbok (1), giraffe (9), hartebeest (12), impala (171), kudu (27), red lechwe (10), reedbuck (1), rhino (2), springbok (5), steenbok (2), warthog (24), waterbuck (1), wildebeest (33), honey badger (1), lion (43), and zebra (21). …

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Preserving And Using Germplasm And Dissociated Embryonic Cells For Conserving Caribbean And Pacific Coral, Mary Hagedorn, Virginia Carter, Kelly Martorana, Malia K. Paresa, Jason Acker, Iliana B. Baums, Eric Borneman, Michael Brittsan, Michael Byers, Michael Henley, Michael Laterveer, Jo Ann Leong, Megan Mccarthy, Stuart Meyers, Brian D. Nelson, Dirk Petersen, Terrence Tiersch, Rafael Cuevas Uribe, Erik Woods, David Wildt Mar 2012

Preserving And Using Germplasm And Dissociated Embryonic Cells For Conserving Caribbean And Pacific Coral, Mary Hagedorn, Virginia Carter, Kelly Martorana, Malia K. Paresa, Jason Acker, Iliana B. Baums, Eric Borneman, Michael Brittsan, Michael Byers, Michael Henley, Michael Laterveer, Jo Ann Leong, Megan Mccarthy, Stuart Meyers, Brian D. Nelson, Dirk Petersen, Terrence Tiersch, Rafael Cuevas Uribe, Erik Woods, David Wildt

Faculty Publications

Coral reefs are experiencing unprecedented degradation due to human activities, and protecting specific reef habitats may not stop this decline, because the most serious threats are global (i.e., climate change), not local. However, ex situ preservation practices can provide safeguards for coral reef conservation. Specifically, modern advances in cryobiology and genome banking could secure existing species and genetic diversity until genotypes can be introduced into rehabilitated habitats. We assessed the feasibility of recovering viable sperm and embryonic cells post-thaw from two coral species, Acropora palmata and Fungia scutaria that have diffferent evolutionary histories, ecological niches and reproductive strategies. In vitro …

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Spike Resonance Properties In Hippocampal O-Lm Cells Are Dependent On Refractory Dynamics., Kispersky Tj, Fernandez Fr, Economo Mn, White Ja Mar 2012

Spike Resonance Properties In Hippocampal O-Lm Cells Are Dependent On Refractory Dynamics., Kispersky Tj, Fernandez Fr, Economo Mn, White Ja

Faculty Publications

No abstract provided.

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Faculty Senate Newsletter, March 2012, Louisiana State University And Agricultural & Mechanical College Mar 2012

Faculty Senate Newsletter, March 2012, Louisiana State University And Agricultural & Mechanical College

LSU Faculty Senate Publications

Message from President: With the advent of the political season and the opening of the Louisiana Legislature, universities up, down, and across our marshy state go public. Lacking competition from football and situated in a media market that generates fewer distractions than do major metropolitan areas, universities and their troubles routinely grab frontpage or top-of-the-hour placement in journalistic productions. Loud and colorful battles, whether against budget woes or recalcitrant lawmakers or consultants with reorganization plans, draw public attention to the big issues surrounding education but also distract public attention from the more subtle causes of university distress, many of which …

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Impact Of The Red Imported Fire Ant, Solenopsis Invicta (Hymenoptera: Formicidae), On Biological Control Of Salvinia Minima (Hydropteridales: Salviniaceae) By Cyrtobagous Salviniae (Coleoptera: Curculionidae), Katherine A. Parys, Seth J. Johnson Mar 2012

Impact Of The Red Imported Fire Ant, Solenopsis Invicta (Hymenoptera: Formicidae), On Biological Control Of Salvinia Minima (Hydropteridales: Salviniaceae) By Cyrtobagous Salviniae (Coleoptera: Curculionidae), Katherine A. Parys, Seth J. Johnson

Faculty Publications

A 2-yr study of the impacts of the red imported fire ant, Solenopsis invicta Buren, on the success of Cyrtobagous salviniae Calder & Sands as a biological control agent of common salvinia, Salvinia minima Baker, was undertaken in southern Louisiana. Floating moats were constructed around 8 release sites where C. salviniae was introduced as a biological control agent. These moats were used to restrict access to the release site by red imported fire ants in half the quadrats. Moating was successful in limiting the number of red imported fire ants in the release sites over the course of the study …

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Pathogen Persistence In The Environment And Insect-Baculovirus Interactions: Disease-Density Thresholds, Epidemic Burnout, And Insect Outbreaks, Emma Fuller, Bret D. Elderd, Greg Dwyer Mar 2012

Pathogen Persistence In The Environment And Insect-Baculovirus Interactions: Disease-Density Thresholds, Epidemic Burnout, And Insect Outbreaks, Emma Fuller, Bret D. Elderd, Greg Dwyer

Faculty Publications

Classical epidemic theory focuses on directly transmitted pathogens, but many pathogens are instead transmitted when hosts encounter infectious particles. Theory has shown that for such diseases pathogen persistence time in the environment can strongly affect disease dynamics, but estimates of persistence time, and consequently tests of the theory, are extremely rare. We consider the consequences of persistence time for the dynamics of the gypsy moth baculovirus, a pathogen transmitted when larvae consume foliage contaminated with particles released from infectious cadavers. Using field-transmission experiments, we are able to estimate persistence time under natural conditions, and inserting our estimates into a standard …

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Tolerance, Fermentation, And Cytokine Expression In Healthy Aged Male C57bl/6j Mice Fed Resistant Starch, June Zhou, Michael J. Keenan, Jeffrey Keller, Sun O. Fernandez-Kim, Paul J. Pistell, Richard T. Tulley, Anne M. Raggio, Li Shen, Hanjie Zhang, Roy J. Martin, Marc R. Blackman Mar 2012

Tolerance, Fermentation, And Cytokine Expression In Healthy Aged Male C57bl/6j Mice Fed Resistant Starch, June Zhou, Michael J. Keenan, Jeffrey Keller, Sun O. Fernandez-Kim, Paul J. Pistell, Richard T. Tulley, Anne M. Raggio, Li Shen, Hanjie Zhang, Roy J. Martin, Marc R. Blackman

Faculty Publications

Health benefits of resistant starch (RS), a dietary fermentable fiber, have been well documented in young, but not in old populations. As the essential step of more comprehensive evaluations of RS on healthy aging, we examined the effects of dietary RS on tolerance, colonic fermentation, and cytokine expression in aged mice. Healthy older (18-20 months) C57BL/6J male mice were fed control, 18% RS, or 36% RS diets for 10weeks. Body weight gain, body composition, and fat pad weights did not differ among the three groups after 10weeks, indicating good tolerance of the RS diet. Fermentation indicators (cecum weights, and cecal …

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Inactivation Of Androgen-Induced Regulator Ard1 Inhibits Androgen Receptor Acetylation And Prostate Tumorigenesis, Zehua Wang, Zemin Wang, Jianhui Guo, Yingchun Li, Jasmin H. Bavarva, Chiping Qian, M. Christiane Brahimi-Horn, Deyong Tan, Wanguo Liu Feb 2012

Inactivation Of Androgen-Induced Regulator Ard1 Inhibits Androgen Receptor Acetylation And Prostate Tumorigenesis, Zehua Wang, Zemin Wang, Jianhui Guo, Yingchun Li, Jasmin H. Bavarva, Chiping Qian, M. Christiane Brahimi-Horn, Deyong Tan, Wanguo Liu

Faculty Publications

Androgen signaling through androgen receptor (AR) is critical for prostate tumorigenesis. Given that AR-mediated gene regulation is enhanced by AR coregulators, inactivation of those coregulators is emerging as a promising therapy for prostate cancer (PCa). Here, weshowthat the N-acetyltransferase arrest-defect 1 protein (ARD1) functions as a unique AR regulator in PCa cells. ARD1 is up-regulated in human PCa cell lines and primary tumor biopsies. The expression of ARD1 was augmented by treatment with synthetic androgen (R1881) unless AR is deficient or is inhibited by AR-specific siRNA or androgen inhibitor bicalutamide (Casodex). Depletion of ARD1 by shRNA suppressed PCa cell proliferation, …

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Epithelial Na + Sodium Channels In Magnocellular Cells Of The Rat Supraoptic And Paraventricular Nuclei, Ryoichi Teruyama, Mayumi Sakuraba, Lori L. Wilson, Narine E.J. Wandrey, William E. Armstrong Feb 2012

Epithelial Na + Sodium Channels In Magnocellular Cells Of The Rat Supraoptic And Paraventricular Nuclei, Ryoichi Teruyama, Mayumi Sakuraba, Lori L. Wilson, Narine E.J. Wandrey, William E. Armstrong

Faculty Publications

The epithelial Na + channels (ENaCs) are present in kidney and contribute to Na + and water homeostasis. All three ENaC subunits (α, β, and γ) were demonstrated in the cardiovascular regulatory centers of the rat brain, including the magnocellular neurons (MNCs) in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). However, the functional significance of ENaCs in vasopressin (VP) and oxytocin (OT) synthesizing MNCs is completely unknown. In this study, we show with immunocytochemical double-labeling that the α-ENaC is colocalized with either VP or OT in MNCs in the SON and PVN. In addition, parvocellular neurons in the …

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Faculty Senate Newsletter, February 2012, Louisiana State University And Agricultural & Mechanical College Feb 2012

Faculty Senate Newsletter, February 2012, Louisiana State University And Agricultural & Mechanical College

LSU Faculty Senate Publications

Message from President: Nearly two months have slipped away since LSU completed a football season that produced an astonishing record of victories: a record that, even without a national championship as garnish, must surely count as a super-size main course in the meatiest of sports. Remarkably, what lingers from the past season is not the sweet aroma of victory but rather something of a bad taste. Football, like most sports, allegorizes the institutions that sponsor it. Identifying the reason that one of the greatest seasons in sports history is creating only a paucity of post-season joy is a good way …

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