Digital Commons Network^™

Suppression Of Rnai By Dsrna-Degrading Rnaseiii Enzymes Of Viruses In Animals And Plants, Isabel Weinheimer, Yaming Jiu, Minna Liisa Rajamäki, Olli Matilainen, Jukka Kallijärvi, Wilmer J. Cuellar, Rui Lu, Mart Saarma, Carina I. Holmberg, Jussi Jäntti, Jari P.T. Valkonen

Faculty Publications

© 2015 Weinheimer et al. Certain RNA and DNA viruses that infect plants, insects, fish or poikilothermic animals encode Class 1 RNaseIII endoribonuclease-like proteins. dsRNA-specific endoribonuclease activity of the RNaseIII of rock bream iridovirus infecting fish and Sweet potato chlorotic stunt crinivirus (SPCSV) infecting plants has been shown. Suppression of the host antiviral RNA interference (RNAi) pathway has been documented with the RNaseIII of SPCSV and Heliothis virescens ascovirus infecting insects. Suppression of RNAi by the viral RNaseIIIs in non-host organisms of different kingdoms is not known. Here we expressed PPR3, the RNaseIII of Pike-perch iridovirus, in the non-hosts Nicotiana …

Co-Evolution Of Proteins And Solutions: Protein Adaptation Versus Cytoprotective Micromolecules And Their Roles In Marine Organisms, Paul H. Yancey, Joseph F. Siebenaller

Faculty Publications

© 2015. Published by The Company of Biologists Ltd. Organisms experience a wide range of environmental factors such as temperature, salinity and hydrostatic pressure, which pose challenges to biochemical processes. Studies on adaptations to such factors have largely focused on macromolecules, especially intrinsic adaptations in protein structure and function. However, micromolecular cosolutes can act as cytoprotectants in the cellular milieu to affect biochemical function and they are now recognized as important extrinsic adaptations. These solutes, both inorganic and organic, have been best characterized as osmolytes, which accumulate to reduce osmotic water loss. Singly, and in combination, many cosolutes have properties …

Standards For Plant Synthetic Biology: A Common Syntax For Exchange Of Dna Parts, Nicola J. Patron, Diego Orzaez, Sylvestre Marillonnet, Heribert Warzecha, Colette Matthewman, Mark Youles, Oleg Raitskin, Aymeric Leveau, Gemma Farré, Christian Rogers, Alison Smith, Julian Hibberd, Alex A.R. Webb, James Locke, Sebastian Schornack, Jim Ajioka, David C. Baulcombe, Cyril Zipfel, Sophien Kamoun, Jonathan D.G. Jones, Hannah Kuhn, Silke Robatzek, H. Peter Van Esse, Dale Sanders, Giles Oldroyd, Cathie Martin, Rob Field, Sarah O'Connor, Samantha Fox, Brande Wulff, Ben Miller, Andy Breakspear, Guru Radhakrishnan

Faculty Publications

© 2015 New Phytologist Trust. Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the …

Diosgenin, 4-Hydroxyisoleucine, And Fiber From Fenugreek: Mechanisms Of Actions And Potential Effects On Metabolic Syndrome, Scott Fuller, Jacqueline M. Stephens

Faculty Publications

© 2015 American Society for Nutrition. Metabolic syndrome and its complications continue to rise in prevalence and show no signs of abating in the immediate future. Therefore, the search for effective treatments is a high priority in biomedical research. Products derived from botanicals have a time-honored history of use in the treatment of metabolic diseases including type 2 diabetes. Trigonella foenum-graecum, commonly known as fenugreek, is an annual herbaceous plant that has been a staple of traditional herbal medicine in many cultures. Although fenugreek has been studied in both clinical and basic research settings, questions remain about its efficacy and …

Fat In Flames: Influence Of Cytokines And Pattern Recognition Receptors On Adipocyte Lipolysis, Ryan W. Grant, Jacqueline M. Stephens

Faculty Publications

© 2015 the American Physiological Society. Adipose tissue has the largest capacity to store energy in the body and provides energy through the release of free fatty acids during times of energy need. Different types of immune cells are recruited to adipose tissue under various physiological conditions, indicating that these cells contribute to the regulation of adipose tissue. One major pathway influenced by a number of immune cells is the release of free fatty acids through lipolysis during both physiological (e.g., cold stress) and pathophysiological processes (e.g., obesity, type 2 diabetes). Adipose tissue expansion during obesity leads to immune cell …

Are Receptor Concentrations Correlated Across Tissues Within Individuals? A Case Study Examining Glucocorticoid And Mineralocorticoid Receptor Binding, Christine R. Lattin, Daniel E. Keniston, J. Michael Reed, L. Michael Romero

Faculty Publications

Copyright © 2015 by the Endocrine Society. Hormone receptors are a necessary (although not sufficient) part of the process through which hormones like corticosterone create physiological responses. However, it is currently unknown to what extent receptor concentrations across different target tissues may be correlated within individual animals. In this study, we examined this question using a large dataset of radioligand binding data for glucocorticoid receptors (GRs) and mineralocorticoid receptors (MRs) in 13 different tissues in the house sparrow (Passer domesticus) (n = 72). Our data revealed that individual house sparrows tended to exhibit higher or lower receptor binding across all …

Discordance Between Morphological And Molecular Species Boundaries Among Caribbean Species Of The Reef Sponge Callyspongia, Melissa B. Debiasse, Michael E. Hellberg

Faculty Publications

© 2015 The Authors. Sponges are among the most species-rich and ecologically important taxa on coral reefs, yet documenting their diversity is difficult due to the simplicity and plasticity of their morphological characters. Genetic attempts to identify species are hampered by the slow rate of mitochondrial sequence evolution characteristic of sponges and some other basal metazoans. Here we determine species boundaries of the Caribbean coral reef sponge genus Callyspongia using a multilocus, model-based approach. Based on sequence data from one mitochondrial (COI), one ribosomal (28S), and two single-copy nuclear protein-coding genes, we found evolutionarily distinct lineages were not concordant with …

Serotonin 5-Ht2 Receptor Activation Prevents Allergic Asthma In A Mouse Model, Felix Nau, Justin Miller, Jordy Saravia, Terry Ahlert, Bangning Yu, Kyle I. Happel, Stephania A. Cormier, Charles D. Nichols

Faculty Publications

© 2015 the American Physiological Society. Asthma is an inflammatory disease of the lung characterized by airways hyper-responsiveness (AHR), inflammation, and mucus hyperproduction. Current main-stream therapies include bronchodilators that relieve bronchoconstriction and inhaled glucocorticoids to reduce inflammation. The small molecule hormone and neurotransmitter serotonin has long been known to be involved in inflammatory processes; however, its precise role in asthma is unknown. We have previously established that activation of serotonin 5-hydroxytryptamine (5-HT)2A receptors has potent anti-inflammatory activity in primary cultures of vascular tissues and in the whole animal in vasculature and gut tissues. The 5-HT2A receptor agonist, (R)-2,5-dimethoxy-4-iodoamphetamine [(R)-DOI] is …

Discovery And Confirmation Of Genes/Proteins Associated With Maize Aflatoxin Resistance, Z. Y. Chen, K. Rajasekaran, R. L. Brown, R. J. Sayler, D. Bhatnagar

Faculty Publications

Maize (Zea mays L.) is one of the major crops susceptible to Aspergillus flavus infection and subsequent aflatoxin contamination. Many earlier studies indicated the roles of kernel proteins, especially constitutively expressed proteins, in maize resistance to A. flavus infection and aflatoxin production. In this review, we examined the past and current efforts in identifying maize genes and proteins from kernel, rachis, and silk tissues that may play an important role in resistance to A. flavus infection and aflatoxin contamination, as well as the efforts in determining the importance or involvement of them in maize resistance through biochemical, molecular and genetics …

Atoxigenic Aspergillus Flavus Biological Control Of Aflatoxin Contamination: What Is The Mechanism?, K. E. Damann

Faculty Publications

The term 'competitive exclusion' involving physical blockage of growth or access of the toxigenic strain to the seed target has been used to describe the mechanism of biological control of aflatoxin contamination. However, recent evidence suggests that a form of intraspecific aflatoxin inhibition requiring growth of the competing strains together during the infection process in such a way that hyphae physically interact or touch is the trigger for preventing induction of aflatoxin synthesis. This direct touch-based inhibition of aflatoxin synthesis is posited to be the mechanistic basis of biological control in this system. Evidence for this idea comes from the …

Structure Activity Relationships Of Biotin Carboxyl Carrier Protein Of E. Coli Acetyl-Coa Carboxylase, Amanda Breaux

Honors Capstones

No abstract provided.

Components, Volume 11 (2015), Lsu Center For Computation And Technology

Components

No abstract provided.

Identification Of Potential Genetic Components Involved In The Deviant Quorum-Sensing Signaling Pathways Of Burkholderia Glumae Through A Functional Genomics Approach, Ruoxi Chen, Inderjit K. Barphagha, Jong Hyun Ham

Faculty Publications

Burkholderia glumae is the chief causal agent for bacterial panicle blight of rice. The acyl-homoserine lactone (AHL)-mediated quorum-sensing (QS) system dependent on a pair of luxI and luxR homologs, tofI and tofR, is the primary cell-to-cell signaling mechanism determining the virulence of this bacterium. Production of toxoflavin, a major virulence factor of B. glumae, is known to be dependent on the tofI/tofR QS system. In our previous study, however, it was observed that B. glumae mutants defective in tofI or tofR produced toxoflavin if they grew on the surface of a solid medium, suggesting that alternative signaling pathways independent of …

Lsu General Catalog 2015-2016, Louisiana State University And Agricultural And Mechanical College

LSU General Catalog

The LSU General Catalog describes all undergraduate and graduate departments and programs with degree requirements and courses offered for each one. The LSU General Catalog includes information on registration and financial aid as well as academic services offered to all students.

Aggressiveness In Inland And Coastal Colonies Of Solenopsis Invicta, Desmarie Stewart

Honors Capstones

No abstract provided.

The Many Chances Of Charles Darwin, Charles H. Pence

Faculty Publications

No abstract provided.

The Rebel Yell: A Cultural History, Rod Andrew Jr.

Civil War Book Review

“The Ugliest Sound that Any Mortal Ever Heard:" The Rebel Yell in American History and Culture

The famous, high-pitched “rebel yell" began its career as a distinctive war cry and a means of communication for Confederate soldiers, and soon became a marker of white southern identity. By th....

Can We Identify Genes With Increased Phylogenetic Reliability?, Vinson P. Doyle, Randee E. Young, Gavin J.P. Naylor, Jeremy M. Brown

Faculty Publications

© The Author(s) 2015. Topological heterogeneity among gene trees is widely observed in phylogenomic analyses and some of this variation is likely caused by systematic error in gene tree estimation. Systematic error can be mitigated by improving models of sequence evolution to account for all evolutionary processes relevant to each gene or identifying those genes whose evolution best conforms to existing models. However, the best method for identifying such genes is not well established. Here, we ask if filtering genes according to their clock-likeness or posterior predictive effect size (PPES, an inference-based measure of model violation) improves phylogenetic reliability and …

Jump-Diffusion Approximation Of Stochastic Reaction Dynamics: Error Bounds And Algorithms, Arnab Ganguly, Derya Altintan, Heinz Koeppl

Faculty Publications

Biochemical processes in living cells are comprised of reactions with vastly varying speeds and molecular counts of the reactant species. Classical deterministic or stochastic approaches to modeling often fail to exploit this multiscale nature of the reaction systems. In this paper, we propose a jump-diffusion approximation to these types of multiscale systems that couples the two traditional modeling approaches. An error bound of the proposed approximation is derived and used to partition the reactions into two sets, where one set is modeled by continuous stochastic differential equations and the other by discrete state processes. The methodology leads to a very …

Manual For Ageing And Sexing Birds Of Bosque Fray Jorge National Park And Northcentral Chile, With Notes On Range And Breeding Seasonality, Peter Pyle, Andrew Engilis, Douglas A. Kelt

Special Publications of the Museum of Natural Science

Bosque Fray Jorge National Park (hereafter "Fray Jorge") comprises 9,959 ha on the coast of Chile’s Region IV (Coquimbo), approximately 400 km north of Santiago and 100 km south of La Serena (30°41’S, 71°40’W) (Fig. 1). It is a Biosphere Reserve and has been protected from grazing and disturbance since 1941 (Squeo et al. 2004). As such, it is a biotic oasis surrounded by agricultural and increasingly disturbed terrain (Bahre 1979). The climate is Mediterranean, with ca. 130 mm of annual precipitation measured since 1989 at an on-site meteorological station, 90% falling in winter (May-Sept). Summers are warm and dry, …

Manual Para Estimar Edad Y Sexo En Aves Del Parque Nacional Bosque Fray Jorge Y Chile Central, Con Notas Sobre Rangos De Distribución Y Estación Reproductiva, Peter Pyle, Andrew Engilis Jr., Douglas A. Kelt

Special Publications of the Museum of Natural Science

El Parque Nacional Fray Jorge (en adelante "Fray Jorge") comprende 9.959 ha. en la costa de la IV Región de Chile (Coquimbo), a unos 400 km al norte de Santiago y a 100 km al sur de La Serena (30 ° 41'S, 71 ° 40'W) (Fig. 1). Se trata de una Reserva de la Biosfera que se ha protegido del pastoreo y las perturbaciones antrópicas desde 1941 (Squeo et al. 2004). Como tal, es un oasis biótico rodeado de áreas agrícolas cada vez más intervenidas por el hombre (Bahre 1979). El clima es mediterráneo, con 130 mm de precipitación anual, …

Birthing The Poet Elizabeth Singer Rowe And The Athenian Mercury, Heidi Laudien

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

The Unending Dunciad Pope's Weird Revenge, Cedric D. Reverand Ii

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Three Bibliopoles, J. T. Scanlan

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

The Arabian Nights And The Oriental Tale Sustainable Enlightenment Texts, Malcolm Jack

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

"Iron War" As "Daily Care"Sustainability And The Dialectic Of Care In Dryden's Georgics, Erin Drew

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

"Go Not Far To Dine" Pedagogical Approaches To Sustainable Consumption In The Eighteenth-Century Studies Classroom And Beyond, Marlon Pareja, Sher Li Ong, Michelle Merrill, Samara Anne Cahill

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

An Eighteenth-Century Plea For Sustainable Forestry Ostervald's Description Des Montagnes & Vallees Du Pays De Neuchatel (1764), Alexandra Cook

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Book Reviews

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Assessing Uncertainty In Coastal Marsh Core Sampling For Waterfowl Foods, Kevin M. Ringelman, Christopher K. Williams, John M. Coluccy

Faculty Publications

Quantifying foraging resources available to waterfowl in different habitat types is important for estimating energetic carrying capacity. To accomplish this, most studies collect soil-core samples from the marsh substrate, sieve and sort food items, and extrapolate energy values to wetland or landscape scales. This is a costly and time-intensive process; furthermore, extrapolation methods yield energy estimates with large variances relative to the mean. From both research and management perspectives, it is important to understand sources of this variation and estimate the number of soil cores needed to reduce the variance to desired levels. Using 2,341 cores collected from freshwater and …