Digital Commons Network^™

Dna Methylomes Of Bovine Gametes And In Vivo Produced Preimplantation Embryos, Zongliang Jiang, Jianan Lin, Hong Dong, Xinbao Zheng, Sadie L. Marjani, Jingyue Duan, Zhengqing Ouyang, Jingbo Chen, Xiuchun Tian

Faculty Publications

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. DNA methylation is an important epigenetic modification that undergoes dynamic changes in mammalian embryogenesis, during which both parental genomes are reprogrammed. Despite the many immunostaining studies that have assessed global methylation, the gene-specific DNA methylation patterns in bovine preimplantation embryos are unknown. Using reduced representation bisulfite sequencing, we determined genome-scale DNA methylation of bovine sperm and individual in vivo developed oocytes and preimplantation embryos. We show that (1) the major wave of genome-wide demethylation was completed by the 8-cell stage; (2) promoter …

An Ethanolic Extract Of Artemisia Scoparia Inhibits Lipolysis In Vivo And Has Antilipolytic Effects On Murine Adipocytes In Vitro, Anik Boudreau, Allison J. Richard, Jasmine A. Burrell, William T. King, Ruth Dunn, Jean Marc Schwarz, David M. Ribnicky, Jennifer Rood, J. Michael Salbaum, Jacqueline M. Stephens

Faculty Publications

© 2018, American Physiological Society. All rights reserved. An ethanolic extract of Artemisia scoparia (SCO) has metabolically favorable effects on adipocyte development and function in vitro and in vivo. In diet-induced obese mice, SCO supplementation significantly reduced fasting glucose and insulin levels. Given the importance of adipocyte lipolysis in metabolic health, we hypothesized that SCO modulates lipolysis in vitro and in vivo. Free fatty acids and glycerol were measured in the sera of mice fed a high-fat diet with or without SCO supplementation. In cultured 3T3-L1 adipocytes, the effects of SCO on lipolysis were assessed by measuring glycerol and free …

Enhancer-Driven Alternative Promoters Of Imprinted Genes, Joomyeong Kim, Bambarendage P.U. Perera, Subash Ghimire

Faculty Publications

© 2018 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In the current study, we characterized the expression and histone modification profiles of the alternative promoters found within imprinted Igf2r, Mest, Zac1, Peg3, Snrpn and non-imprinted Myc loci. In terms of expression pattern, the alternative promoters are highly tissue-specific, which is in a stark contrast to the ubiquitous expression of the corresponding main promoters. The alternative promoters are associated with the …

A Discrete Element Method Study Of Granular Self-Organization In An Orbital-Driven Sphere And Computational Fluid Dynamics Simulations Of Slurry Flow In A Single-Loop Loop Reactor, Spencer Blum

Honors Capstones

No abstract provided.

Molecular Level Interactions And Transformations Of Natural Organic Matter Within The Environment, Benjamin J. Haywood

LSU Doctoral Dissertations

This work was done in order to deepen the understanding of interactions occurring during sorption of anthropogenic organic compounds and dissolved organic matter (DOM) onto soil. As well as the transformation DOM undergoes during the sorption process and the overall transformation OM undergoes in the environment. This was achieved through the use of a “bottom-up” synthetic investigation, “top-down” natural system study, and a combination of these approaches.

The “bottom-up” approach, discussed in Chapter 2, involved the synthesis of a modular multi-tiered engineered soil surrogate (ESS) designed to mimic OM and general trends identified in the literature that impacts sorption behavior. …

Intestinal Microbiome Composition Of An Acute In Vivo Alzheimer’S Disease Rat Model, Jimmy Saravia

Honors Capstones

No abstract provided.

Design And Application Of Task-Specific Gumbos And Nanogumbos For Sensing And Separation, Mingyan Cong

LSU Doctoral Dissertations

The work presented in this dissertation employs task-specific materials for sensing and protein separation applications. These materials were derived from a group of uniform materials based on organic salts (GUMBOS). GUMBOS are organic salts similar to ionic liquids, but have melting points ranging from 25 to 250 °C. As with ionic liquids, the properties of GUMBOS can be easily tuned by changing the counter-ion. Thus, task-specific GUMBOS can be designed and prepared with properties that are beneficial for applications in sensing or protein separation. In this dissertation, the selective responsive behavior of a series of GUMBOS and nanomaterials derived from …

From The River To The Gulf: An Investigation Of Biogeochemical Cycling In Wetland Soils And Coastal Shelf Sediments, Katie Bowes

LSU Master's Theses

Louisiana is home to 40% of the coastal wetlands in the lower 48 states, yet accounts for 80% of the coastal wetland loss in this region. This loss is attributed to decreased sediment supply, levee alteration, sea level rise, channelization, and subsidence. The levee system in Louisiana disconnected coastal wetlands from the main stem of the Mississippi River (MSR), reducing the amount of land-building sediment that reaches coastal wetlands. This disconnection also allows a greater percentage of river discharge, including agricultural contaminants and runoff, to flow into the northern Gulf of Mexico (NGOM).

The 2012 Coastal Master Plan proposed eight …

Calculation Guided Rational Design And Synthesis Of Novel Cationic Fluorescent Meso–Pyridinium Bodipys For Bio-Imaging, Daniel J. Lamaster

LSU Doctoral Dissertations

Chapter 1 contains a brief overview of the history, synthesis, and properties of BODIPY dyes as well as that of the biomedical applications of bio-imaging and photodynamic therapy. Additionally, an overview of the theoretical framework of density functional theory and its time-dependent variant are provided. In Chapter 2, the effects of structural modification on the electronic structure and electron dynamics of cationic meso-(4-pyridyl)-BODIPYs were investigated. A library of 2,6-difunctionalized meso-(4-pyridyl)-BODIPYs bearing various electron-withdrawing substituents was designed and DFT calculations were used to model the redox properties, while TDDFT was used to determine the effects of functionalization on the excited …

Effect Of Heat Stress On Bovine Follicular Development And Epigenetic Changes, Fabian Andres Diaz

LSU Doctoral Dissertations

Climate is one of the most important limiting factors in animal production. Cattle under the effect of heat stress have reduced fertility. Negative effects during gamete maturation and embryo development have been observed at the morphological, biochemical, transcriptional and developmental levels. Epigenetic mechanisms play a fundamental role in the regulation of gamete and embryo development. Several genes activated during gamete maturation and early embryo development are controlled by epigenetic mechanisms. There are no studies evaluating the effect of heat-stress on the epigenetic profile of bovine oocytes and embryos. Similarly, there is no information on the effect of heat stress on …

Scaffolded Play As A Predictor Of Change In Language And Play In Children With Autism, Cara Tyson

LSU Doctoral Dissertations

A child’s play is facilitative of language development, creating a situation where words are needed to speak to and for toy characters, socially engage with peers, and develop play narratives. Children with language disorders, in particular autism spectrum disorder, often fail to engage in play activities at levels of their typically developing peers. However, under highly structured conditions, or scaffolded conditions, children with autism have demonstrated the ability to engage in play at similar levels as their typically developing peers. It remains unclear whether direct teaching of play under scaffolded conditions will predict language skills over time for this population. …

Longitudinal Study Of The Attitudes And Behaviors Of Environmental Education Program Participants., Kasie R. Dugas

LSU Master's Theses

The goal of this longitudinal study was to measure the attitudes and behaviors of participants of the Marsh Maneuvers program over the course of the last 25 years. The goal of the Marsh Maneuvers program is to educate students into becoming environmentally literate adults. Environmental literacy is considered herein to be people possessing knowledge about and positive attitudes and behaviors towards the environment. Although students learn basic science and environmental knowledge in the classroom, true environmental literacy is believed to require more hands-on understanding of the material. To evaluate the effectiveness of the program, the following three objectives were completed. …

Course Design…Online: Helping Students Perform In The Digital Age, Joseph W. Harris

LSU Doctoral Dissertations

The current study sought to test the relationship between course design, as described by the rubric produced by Quality Matters, and online university student performance. Due to the link between student motivation and active learning behaviors, and thus performance, it was predicted that the better-designed courses would facilitate student motivation. It was also predicted that goal orientation would moderate this relationship. While a significant relationship was observed between student motivation and course performance, no relationship was observed between course quality, as measured by the QM Rubric, and motivation, or performance. Only slight evidence was found for a moderating effect of …

Analysis Of The Soil Health Of Pastures Over-Seeded With Winter Annuals, Kathleen M. Bridges

LSU Doctoral Dissertations

Utilizing winter annuals in livestock pastures reduces feed costs of hay, and provides additional vegetative cover that is beneficial to the soil. However, how this practice affects the soil microbial communities has not been well studied. Two sites were included in this study. Both were commercial cattle grazing operations that over-seeded a mixture of grasses, legumes, and brassicas into pastures dominated by bahiagrass (Paspalum notatum). Soil health was assessed using a suite of physical, chemical, and biological properties. Soil physical assessments included bulk density and aggregate stability. Soil chemical assessments included macronutrients, pH, soil organic matter (SOM), total …

Synergistic Effects Of Temperature And Salinity On The Gene Expression And Physiology Of Crassostrea Virginica, Hollis Jones

LSU Master's Theses

Crassostrea virginica, the eastern oyster, forms reefs that provide critical services and benefits to the resiliency of the surrounding ecosystem. Changes in environmental conditions, including salinity and temperature, can dramatically alter the services oysters provide by affecting their population dynamics. Climate warming may further exacerbate the effects of salinity changes as precipitation events increase in frequency, intensity, and duration. Temperature and salinity independently and synergistically influence gene expression and physiology in marine organisms. We used comparative transcriptomics, physiology, and a field assessment experiment to investigate whether Louisianan oyster are changing their phenotypes to cope with increased temperature and salinity …

Tidal-, Wind-, And Buoyancy-Driven Dynamics In The Barataria Estuary And Its Impact On Estuarine-Shelf Exchange Processes, Linlin Cui

LSU Doctoral Dissertations

A three-dimensional, high-resolution, Finite-Volume Coastal Ocean Model (FVCOM) was used to study the dynamics of Barataria Estuary located in the Southeastern Louisiana. Three numerical experiments with different discharge scenarios, including the actual discharge (average ~ 160 m³ s^-1) from the Davis Pond Diversion (DPD) over three months from April to June 2010, no discharge (NO), and the proposed Mid-Barataria Diversion (MBD) with a constant discharge of 850 m³ s^-1, were conducted to investigate the impacts of river diversions on salinity gradients and residence times in the estuary. The three-month average salinity indicated that surface …

First-Principles Study On The Catalytic Role Of Cerium Dioxide In The Conversion Of Organic Compounds, Chuanlin Zhao

LSU Doctoral Dissertations

Ceria is an earth-abundant material that has been widely used in heterogeneous catalysis, environmental catalysis, and energy applications thanks for its ability to readily convert between different oxidation states. The objective of this study is to theoretically elucidate the reaction mechanisms for the conversion of model organic compounds on ceria, in order to gain insights for the design of cost-effective and selective ceria-based catalysts. Acetaldehyde, acetic acid, and para-nitrophenyl phosphate monoester were selected as the model compounds to probe ceria surfaces. Density functional theory calculations can provide accurate predictions of adsorption and reaction energetics, which can be used to …

Soybean Response To Dicamba: Associated Injury Criteria And Development Of A Model To Predict Yield Loss, Matthew Ryan Foster

LSU Doctoral Dissertations

In research conducted using indeterminate soybean [Glycine max (L.) Merr.], fourteen injury criteria observed following dicamba at 0.6 to 280 g ae ha^-1 (1/1000 to ½ of 560 g ha^-1 use rate) were rated using a scale of 0= no injury, 1= slight, 2= slight to moderate, 3= moderate, 4= moderate to severe, and 5= severe. Greatest crop injury 15 d after treatment (DAT) was observed following dicamba applied at 0.6 to 4.4 g ha^-1 at V3/V4 for upper canopy pale leaf margins (3.8 to 4.2) and at R1/R2 for terminal leaf cupping (4.1 to 5.0) …

Thermal And Mechanical Energy Harvesting Using Lead Sulfide Colloidal Quantum Dots, Taher Ghomian

LSU Doctoral Dissertations

The human body is an abundant source of energy in the form of heat and mechanical movement. The ability to harvest this energy can be useful for supplying low-consumption wearable and implantable devices. Thermoelectric materials are usually used to harvest human body heat for wearable devices; however, thermoelectric generators require temperature gradient across the device to perform appropriately. Since they need to attach to the heat source to absorb the heat, temperature equalization decreases their efficiencies. Moreover, the electrostatic energy harvester, working based on the variable capacitor structure, is the most compatible candidate for harvesting low-frequency-movement of the human body. …

The Use Of Systematic Distractions To Increase Sustained Attention In School-Aged Children With Attention Problems, Emma Larson

LSU Master's Theses

With the growing prevalence of Attention Deficit Hyperactivity Disorder (ADHD) in children (DSM-5, 2013), it has been established that the ability of these children to sustain attention is marked with difficulty (Barkley, 2006). Prior research has addressed the need to increase sustained attention in children with ADHD, however, not all of the promising methods take into consideration that children with ADHD have difficulty ignoring distractions (Rapport et al., 2009). This is problematic because the typical classroom environment is filled with distractions. The current study attempted to fill this research gap by training participants to sustain attention while systematically introducing distractions …

An Evaluation Of Sciaenid Growth In The Gulf Of Mexico, Shane Flinn

LSU Master's Theses

Growth models estimate life history parameters that are used in the management of fisheries stocks. The most commonly used growth model in fisheries is the von Bertalanffy growth model (VBGM), yet it has been shown to provide a poor fit for length-at-age data of some species and other models exist. I reviewed 196 peer-reviewed age and growth studies and 50 NOAA stock assessments to examine temporal trends in the use of growth models and model selection in fisheries. I found that the use of multi-model frameworks has increased since the year 2000 and information theoretic approaches are replacing goodness-of-fit and …

Crash/Near-Crash: Impact Of Secondary Tasks And Real-Time Detection Of Distracted Driving, Peter Ramzy Zaki Bakhit

LSU Doctoral Dissertations

The main goal of this dissertation is to investigate the problem of distracted driving from two different perspectives. First, the identification of possible sources of distraction and their associated crash/near-crash risk. That can assist government officials toward more informed decision-making process, allowing for optimized allocation of available resources to reduce roadway crashes and improve traffic safety. Second, actively counteracting the distracted driving phenomenon by quantitative evaluation of eye glance patterns.

This dissertation research consists of two different parts. The first part provides an in-depth analysis for the increased crash/near-crash risk associated with different secondary task activities using the largest real-world …

Aldosterone Mediated Regulation Of Epithelial Sodium Channel (Enac) Subunits In The Rat Hypothalamus, Natalie J. Mills, Kaustubh Sharma, Masudul Haque, Meagan Moore, Ryoichi Teruyama

Faculty Publications

© 2018 IBRO Current evidence suggests that the epithelial Na + channel (ENaC) in the brain plays a significant role in the development of hypertension. ENaC is present in vasopressin (VP) neurons in the hypothalamus, suggesting that ENaC in VP neurons is involved in the regulation of blood pressure. Our recent study demonstrated that high dietary salt intake caused an increase in the expression and activity of ENaC that were responsible for the more depolarized basal membrane potential in VP neurons. A known regulator of ENaC expression, the mineralocorticoid receptor (MR), is present in VP neurons, suggesting that ENaC expression …

Genome-Wide Screening Identifies Genes And Biological Processes Implicated In Chemoresistance And Oncogene-Induced Apoptosis, Tengyu Ko

LSU Doctoral Dissertations

Anti-proliferative responses such as senescence and apoptosis are often used by normal cells to combat oncogenic insults and to prevent tumorigenesis. However, oncogenic mutations are frequently found in cancers, suggesting that additional mutations may occur to facilitate the bypass of these anti-proliferative responses. It is believed that some of these additional mutations may also contribute to the chemoresistance of cancers. This dissertation focused on identifying novel genes and biological processes implicated in chemoresistance and tumorigenesis.

Cisplatin-based chemotherapeutic regimens are frequently used for treatments of solid tumors. However, tumor cells may have inherent or acquired resistance to cisplatin, and the underlying …

Cactusnets: Layer Applicability As A Metric For Transfer Learning, Edward Collier, Robert Dibiano, Supratik Mukhopadhyay

Faculty Publications

Deep neural networks trained over large datasets learn features that are both generic to the whole dataset, and specific to individual classes in the dataset. Learned features tend towards generic in the lower layers and specific in the higher layers of a network. Methods like fine-tuning are made possible because of the ability for one filter to apply to multiple target classes. Much like the human brain this behavior, can also be used to cluster and separate classes. However, to the best of our knowledge there is no metric for how applicable learned features are to specific classes. In this …

A Review Of Current Knowledge Concerning The Breeding And Summer Distribution Of The Cordilleran Flycatcher (Empidonax Occidentalis) In Mexico, Harold F. Greeney, Matthew J. Miller, Charles Van Riper Iii

Occasional Papers of the Museum of Natural Science, Louisiana State University

The Cordilleran Flycatcher (Empidonax occidentalis) is one of many North American birds whose distribution crosses the US–Mexican border and for which little is presently known about summer distribution and breeding biology south of this border. In addition, the Cordilleran Flycatcher presents ornithologists with a number of challenging questions surrounding its taxonomy, migration, distribution, population structure, and species limits. In particular, there remains a good deal of uncertainty concerning the species-level relationships, seasonal movements, and the wintering and breeding distributions of both recognized subspecies of Cordilleran Flycatcher in Mexico. Using field observations, a thorough review of the literature, and …

Fibroblast Growth Factor 21, Adiposity, And Macronutrient Balance In A Healthy, Pregnant Population With Overweight And Obesity, Elizabeth F. Sutton, Christopher D. Morrison, Jacqueline M. Stephens, Leanne M. Redman

Faculty Publications

© 2018, © 2018 Taylor & Francis. Aim of the study: The regulation and actions of fibroblast growth factor 21 (FGF21) are responsive to energy status and macronutrient balance, and investigations of FGF21 in normal pregnancy, which could be informative for FGF21 biology, are seldom. The goal of our study was to examine FGF21 levels in a contemporary healthy, pregnant population. Methods: We phenotyped 43 women with overweight and obesity during pregnancy for weight, body composition, and fasting blood. Serum FGF21 was measured during the first and third trimesters. Placentas were collected at delivery. Results: Maternal FGF21 concentrations were positively …

Contact-Type Endoscope Sers Probe, And Related Methods, Martin Feldman, Dooyoung Hah

College of Engineering Patents

A contact-type endoscope surface enhanced Raman scattering (SERS) probe includes a gradient-index (GRIN) lens, a transparent substrate adhered to the GRIN lens, and a rough metallic layer adhered to an opposite side of the transparent substrate from the GRIN lens. The GRIN lens focuses light from a Raman spectrometer onto the rough metallic layer, and the rough metallic layer is positioned at the distal end of the contact-type endoscope SERS probe.

3d Mri Modeling Of Thin And Spatially Complex Soft Tissue Structures Without Shrinkage: Lamprey Myosepta As An Example, Bradley M. Wood, Guang Jia, Owen Carmichael, Kevin Mcklveen, Dominique G. Homberger

Faculty Publications

© 2018 Wiley Periodicals, Inc. 3D imaging techniques enable the nondestructive analysis and modeling of complex structures. Among these, MRI exhibits good soft tissue contrast, but is currently less commonly used for nonclinical research than X-ray CT, even though the latter requires contrast-staining that shrinks and distorts soft tissues. When the objective is the creation of a realistic and complete 3D model of soft tissue structures, MRI data are more demanding to acquire and visualize and require extensive post-processing because they comprise noncubic voxels with dimensions that represent a trade-off between tissue contrast and image resolution. Therefore, thin soft tissue …

Spreading The News About The Novel Conjugation Mechanism In Streptomyces Bacteria, Gregg S. Pettis

Faculty Publications

© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd The hallmark of mycelial spore-forming bacteria of the genus Streptomyces is their prolific production of antibiotics and other bioactive secondary metabolites as part of a complex morphological and physiological developmental program. They are further distinguished by a conjugation mechanism that differs substantially from the single-strand mode of DNA transfer via Type IV secretion, which is exhibited by numerous unicellular Gram-negative and Gram-positive bacteria. At the crux of the novel intermycelial transfer event in Streptomyces spp. is a membrane pore composed of a single plasmid protein (TraB), which also …