Digital Commons Network^™

The Language Of Rats: Unwelcome Animals And Interspecies Connection In Contemporary Anglophone Fiction, Kieran Leigh Lyons

LSU Doctoral Dissertations

The Language of Rats: Unwelcome Animals and Interspecies Connection in Global Contemporary Fiction consists of three essays examining the representation of what I call unwelcome animals in contemporary Anglophone novels from the United States, Nigeria, and India. These animals often live alongside humans yet are perceived as threats or annoyances. Literary depictions of this fraught relationship reveal, and sometimes critique, the intellectual structures that shape how we understand and represent interspecies connections. This dissertation contributes to our understanding of the interspecies dimensions of contemporary fiction by bringing together the fields of environmental criticism, animal studies, postcolonialism, and U.S. Southern studies. …

Development Of A Surgical Procedure For Removal Of A Placentome From A Pregnant Ewe During Gestation, Colleen A. Lambo, Ashley K. Edwards, Fuller W. Bazer, Kathrin Dunlap, M. Carey Satterfield

Faculty Publications

Background: In recent decades, there has been a growing interest in the impact of insults during pregnancy on postnatal health and disease. It is known that changes in placental development can impact fetal growth and subsequent susceptibility to adult onset diseases; however, a method to collect sufficient placental tissues for both histological and gene expression analyses during gestation without compromising the pregnancy has not been described. The ewe is an established biomedical model for the study of fetal development. Due to its cotyledonary placental type, the sheep has potential for surgical removal of materno-fetal exchange tissues, i.e., placentomes. A novel …

Cardiac Fibrosis And Cardiac Fibroblast Lineage-Tracing: Recent Advances, Xing Fu, Qianglin Liu, Chaoyang Li, Yuxia Li, Leshan Wang

Faculty Publications

© Copyright © 2020 Fu, Liu, Li, Li and Wang. Cardiac fibrosis is a common pathological change associated with cardiac injuries and diseases. Even though the accumulation of collagens and other extracellular matrix (ECM) proteins may have some protective effects in certain situations, prolonged fibrosis usually negatively affects cardiac function and often leads to deleterious consequences. While the development of cardiac fibrosis involves several cell types, the major source of ECM proteins is cardiac fibroblast. The high plasticity of cardiac fibroblasts enables them to quickly change their behaviors in response to injury and transition between several differentiation states. However, the …

Disturbance Legacies Increase And Synchronize Nutrient Concentrations And Bacterial Productivity In Coastal Ecosystems, John S. Kominoski, Evelyn E. Gaiser, Edward Castañeda-Moya, Stephen E. Davis, Shimelis B. Dessu, Paul Julian, Dong Yoon Lee, Luca Marazzi, Victor H. Rivera-Monroy, Andres Sola, Ulrich Stingl, Sandro Stumpf, Donatto Surratt, Rafael Travieso, Tiffany G. Troxler

Faculty Publications

Long-term ecological research can resolve effects of disturbance on ecosystem dynamics by capturing the scale of disturbance and interactions with environmental changes. To quantify how disturbances interact with long-term directional changes (sea-level rise, freshwater restoration), we studied 17 yr of monthly dissolved organic carbon (DOC), total nitrogen (TN), and phosphorus (TP) concentrations and bacterioplankton productivity across freshwater-to-marine estuary gradients exposed to multiple disturbance events (e.g., droughts, fire, hurricanes, and low-temperature anomalies) and long-term increases in water levels. By studying two neighboring drainages that differ in hydrologic connectivity, we additionally tested how disturbance legacies are shaped by hydrologic connectivity. We predicted …

Long-Term Trajectory Of Nitrogen Loading And Delivery From Mississippi River Basin To The Gulf Of Mexico, Hanqin Tian, Rongting Xu, Shufen Pan, Yuanzhi Yao, Zihao Bian, Wei Jun Cai, Charles S. Hopkinson, Dubravko Justic, Steven Lohrenz, Chaoqun Lu, Wei Ren, Jia Yang

Faculty Publications

The large areal extent of hypoxia in the northern Gulf of Mexico has been partially attributed to substantial nitrogen (N) loading from the Mississippi River basin, which is driven by multiple natural and human factors. The available water quality monitoring data and most of the current models are insufficient to fully quantify N load magnitude and the underlying controls. Here we use a process-based Dynamic Land Ecosystem Model to examine how multiple factors (synthetic N fertilizer, atmospheric N deposition, land use changes, climate variability, and increasing atmospheric CO2) have affected the loading and delivery of total nitrogen (TN) consisting of …

Bionoinet: Ligand-Binding Site Classification With Off-The-Shelf Deep Neural Network, Wentao Shi, Jeffrey M. Lemoine, Abd El Monsif A. Shawky, Manali Singha, Limeng Pu, Shuangyan Yang, J. Ramanujam, Michal Brylinski

Faculty Publications

© The 2020 Author(s). Published by Oxford University Press. All rights reserved. Motivation: Fast and accurate classification of ligand-binding sites in proteins with respect to the class of binding molecules is invaluable not only to the automatic functional annotation of large datasets of protein structures but also to projects in protein evolution, protein engineering and drug development. Deep learning techniques, which have already been successfully applied to address challenging problems across various fields, are inherently suitable to classify ligand-binding pockets. Our goal is to demonstrate that off-the-shelf deep learning models can be employed with minimum development effort to recognize nucleotide-and …

Population Epigenetic Divergence Exceeds Genetic Divergence In The Eastern Oyster Crassostrea Virginica In The Northern Gulf Of Mexico, Kevin M. Johnson, Morgan W. Kelly

Faculty Publications

© 2019 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd Populations may respond to environmental heterogeneity via evolutionary divergence or phenotypic plasticity. While evolutionary divergence occurs through DNA sequence differences among populations, plastic divergence among populations may be generated by changes in the epigenome. Here, we present the results of a genome-wide comparison of DNA methylation patterns and genetic structure among four populations of Eastern oyster (Crassostrea virginica) in the northern Gulf of Mexico. We used a combination of restriction site-associated DNA sequencing (RADseq) and reduced representation bisulfite sequencing (RRBS) to explore population structure, gene-wide averages of …

Cave-Adapted Evolution In The North American Amblyopsid Fishes Inferred Using Phylogenomics And Geometric Morphometrics, Pamela B. Hart, Matthew L. Niemiller, Edward D. Burress, Jonathan W. Armbruster, William B. Ludt, Prosanta Chakrabarty

Faculty Publications

© 2020 The Authors. Evolution © 2020 The Society for the Study of Evolution. Cave adaptation has evolved repeatedly across the Tree of Life, famously leading to pigmentation and eye degeneration and loss, yet its macroevolutionary implications remain poorly understood. We use the North American amblyopsid fishes, a family spanning a wide degree of cave adaptation, to examine the impact of cave specialization on the modes and tempo of evolution. We reconstruct evolutionary relationships using ultraconserved element loci, estimate the ancestral histories of eye-state, and examine the impact of cave adaptation on body shape evolution. Our phylogenomic analyses provide a …

Consuming A Ketogenic Diet Leads To Altered Hypoglycemiccounter-Regulation In Mice, Christopher D. Morrison, Cristal M. Hill, Marina A. Duvall, Carolyn E. Coulter, Jasmin L. Gosey, Matthew J. Herrera, Lauren E. Maisano, Hunter X. Sikaffy, David H. Mcdougal

Division of Basic Science Faculty Publications

Ketogenic diets (KDs) are becoming increasingly popular for the treatment of diabetes, yet they are associated with increased frequency of hypoglycemia. Here we report that mice fed a KD display blunted glucagon release to hypoglycemia and neuroglucopenia, suggesting that consuming a KD may increase the risk for iatrogenic hypoglycemia.

Comprehensive Assessment Of Mitochondrial Respiratory Function In Freshly Isolated Nephron Segments, Allison Mccrimmon, Mark Domondon, Regina F. Sultanova, Daria V. Ilatovskaya, Krisztian Stadler

Division of Basic Science Faculty Publications

Changes in mitochondrial function are central to many forms of kidney disease, including acute injury, diabetic nephropathy, hypertension, and chronic kidney diseases. As such, there is an increasing need for reliable and fast methods for assessing mitochondrial respiratory function in renal cells. Despite being indispensable for many mechanistic studies, cultured cells or isolated mitochondria, however, often do not recapitulate in vivo or close to in vivo situations. Cultured and/or immortalized cells often change their bioenergetic profile and phenotype compared with in vivo or ex vivo situations, and isolated mitochondria are simply removed from their cellular milieu. This is especially important …

A Pomc-Originated Circuit Regulates Stress-Induced Hypophagia, Depression, And Anhedonia, Na Qu, Yanlin He, Chunmei Wang, Pingwen Xu, Yongjie Yang, Xing Cai, Hesong Liu, Kaifan Yu, Zhou Pei, Ilirjana Hyseni, Zheng Sun, Makoto Fukuda, Yi Li, Qing Tian, Yong Xu

Division of Basic Science Faculty Publications

Chronic stress causes dysregulations of mood and energy homeostasis, but the neurocircuitry underlying these alterations remain to be fully elucidated. Here we demonstrate that chronic restraint stress in mice results in hyperactivity of pro-opiomelanocortin neurons in the arcuate nucleus of the hypothalamus (POMCARH neurons) associated with decreased neural activities of dopamine neurons in the ventral tegmental area (DAVTA neurons). We further revealed that POMCARH neurons project to the VTA and provide an inhibitory tone to DAVTA neurons via both direct and indirect neurotransmissions. Finally, we show that photoinhibition of the POMCARH→VTA circuit in mice increases body weight and food intake, …

Direct Measurement Of Sweetpotato Surface Area And Volume Using A Low-Cost 3d Scanner For Identification Of Shape Features Related To Processing Product Recovery, Arthur Villordon, Jeffrey C. Gregorie, Don Labonte

Faculty Publications

The growing demand for sweetpotato French fry and other processed products has increased the need for producing storage roots of desired shape profile (i.e., blocky and less tapered). Length-width ratio (LW) is the current de facto standard for characterizing sweetpotato shape. AlthoughLWis sensitive and descriptive of some types of shape variability, this index may be inadequate to measure taper and other subtle shape variations. Prior work has shown that surface area (SA) and volume (VOL) are important shape descriptors but current direct measurement methods are tedious, inconsistent, and often destructive. A low-cost three-dimensional (3D) scanner was used to acquire digital …

Conserved And Variable Responses Of The Gut Microbiome To Resistant Starch Type 2, Zachary A. Bendiks, Knud E.B. Knudsen, Michael J. Keenan, Maria L. Marco

Faculty Publications

Resistant starch type 2 (RS2), a dietary fiber comprised solely of glucose, has been extensively studied in clinical trials and animal models for its capacity to improve metabolic and systemic health. Because the health modulatory effects of RS2 and other dietary fibers are thought to occur through modification of the gut microbiome, those studies frequently include assessments of RS2-mediated changes to intestinal microbial composition and function. In this review, we identify the conserved responses of the gut microbiome among 13 human and 35 animal RS2 intervention studies. Consistent outcomes of RS2 interventions include reductions in bacterial α-diversity; increased production of …

Characterizing The Epidemiology Of Bluetongue And Epizootic Hemorrhagic Disease Virus In Louisiana, Michael Edward Becker

LSU Doctoral Dissertations

Bluetongue virus (BTV) and Epizootic Hemorrhagic Disease Virus (EHDV) are arthropod-borne viruses (Reoviridae; Orbivirus) transmitted by biting midges in the genus Culicoides. These viruses cause hemorrhagic disease and induce serious morbidity and mortality rates in wild and captive deer. Cattle are normally asymptomatic but serve as the reservoir/amplifying host for these viruses and play an important role in the transmission cycle. The objectives of this study were to characterize the epidemiology of these viruses as they relate to white-tailed deer and cattle, evaluate different trap types for collecting Culicoides midges, and determine if these viruses can be detected …

Convergent Evolution Of Olfactory And Thermoregulatory Capacities In Small Amphibious Mammals, Quentin Martinez, Julien Clavel, Jacob A. Esselstyn, Anang S. Achmadi, Camille Grohé, Nelly Pirot, Pierre Henri Fabre

Faculty Publications

Olfaction and thermoregulation are key functions for mammals. The former is critical to feeding, mating, and predator avoidance behaviors, while the latter is essential for homeothermy. Aquatic and amphibious mammals face olfactory and thermoregulatory challenges not generally encountered by terrestrial species. In mammals, the nasal cavity houses a bony system supporting soft tissues and sensory organs implicated in either olfactory or thermoregulatory functions. It is hypothesized that to cope with aquatic environments, amphibious mammals have expanded their thermoregulatory capacity at the expense of their olfactory system. We investigated the evolutionary history of this potential trade-off using a comparative dataset of …

Heidegger's Phenomenology: 1919-1929, Joseph Arthur Giavotella

LSU Master's Theses

In this work, I show Martin Heidegger’s development of the phenomenological method from 1919 to 1929 as his main approach to all philosophical inquiry. In Chapter 1: Phenomenology as the Hermeneutics of Factical Life, I first show how Heidegger begins his philosophical career in 1919 with lectures that describe phenomenology as an ‘original science’ that seeks to study the structural character of life in itself. Through the four sub-sections of Chapter 1, I show how Heidegger continues to formulate distinct stages of phenomenological methodology through these early lectures that aid in his task to continue the explication of life through …

Dna Methylation Analysis Of Ecr3 Of Oxytocin Receptor Gene, Amanda Husein

Honors Capstones

No abstract provided.

Establishment Of Laboratory Methods To Assess The Application Of Blood Transfusions In Reptiles, Anke Cordula Stoehr

LSU Master's Theses

A broad knowledge base on veterinary transfusion medicine has been developed in common mammalian species, but little is known in reptiles. The goals of this thesis were to establish laboratory methods to assess the application of blood transfusions in reptiles. Morphological and physiological differences in reptiles, including their erythrocytes, hamper direct adaption of mammalian laboratory methods.

Quantification of percentage hemolysis is a crucial tool for quality assessment of stored red blood cell products. We evaluated measurement of the required analytes for this calculation (supernatant and total hemoglobin) with different methods in blood from American alligators and found that the use …

Moche Juvenile Burial Patterns, Audrey J. Deluca

LSU Master's Theses

This thesis examines Moche juvenile burial patterns as documented in the published literature. Reports of cemeteries and other burial excavations were compiled in order to identify the position of children in Moche society as well as ideology surrounding children and childhood. The data collected spans six valleys and fourteen archaeological sites along the north coast of Peru. This investigation revealed 191 juvenile burials dating from A.D. 200 – 850. The variables documented for each burial include site, period, age, sex, burial position, orientation, burial encasing, and description of grave goods, as well as documenting adult individuals buried with juveniles. This …

Virtual Touch: Embodied Experiences Of (Dis)Embodied Intimacy In Mediatized Performance, Naomi Petrea Bennett

LSU Doctoral Dissertations

In this dissertation, I explore a phenomenon I call virtual touch, in which embodied sensations of touch are felt through non-tactile senses. In the digital age, online interactivity has expanded the ways in which individuals experience connection, intimacy, and touch. Digital media, which have traditionally been thought of as disembodied, nevertheless have the ability to elicit intense feelings of touch. Through analysis of digital and virtual installation art, I examine the ways that non-tactile touch remains rooted in the embodied experience. The works I include in this study create a feeling of virtual touch through a co-functioning of the …

Analyzing Climate Risk To Maritime Cypress Swamps And Pine Savannas Of The Gulf Of Mexico Coastal Plain, Clay Stephens Tucker

LSU Doctoral Dissertations

As sea levels rise, coastal trees are increasingly stressed by saltwater inundation, and stressed trees are ideal for studying changes in the environment through time. Dendrochronology has been used for more than a century to explain changes in past environments using tree rings. Using ring widths and stable isotopes from coastal trees, the effects of tropical cyclones and freshwater moisture availability are shown in regard to tree growth and ultimately are discussed in terms of change to the environment as a whole. Changes in tree growth as they relate to various climate parameters are measured using superposed epoch analysis, moving-window …

An Atmospheric And Spatiotemporal Examination Of Lightning-Initiated Terrestrial Gamma-Ray Flashes Detected By The Fermi Satellite And Tetra Ii, Deirdre Colleen Smith

LSU Doctoral Dissertations

Terrestrial Gamma-ray Flashes (TGFs) are sub-millisecond bursts of the highest naturally occurring light-energy found within Earth’s atmosphere. TGFs are associated with the electric fields produced in thunderstorms and are geolocated by coincident sferics from lightning strokes. Though billions of lightning strokes occur globally each year, fewer than 1,000 TGFs are detected via satellite and ground-based sensors and only a small fraction are geolocated via sferics.

To date, few studies have focused on individual thunderstorms and climates that produce TGFs. This dissertation examines TGFs from two differing data samples: 1) NASA's Fermi Gamma-Ray Burst Monitor (2013-2018) and 2) The TGF and …

Understanding The Impact Of Psychological Empowerment, Workplace Motivation, And Social Capital On The Job Performance Of Farmers In Honduras: A Mixed Methods Study, Susan Lale Karimiha

LSU Doctoral Dissertations

A farmer’s job performance is critical to the production of raw materials such as food, fiber, and fuel and is therefore an important concern for individuals, businesses, and economies across the world. The literature on improving farmers’ job performance has focused more on introducing new technologies, and less on the psychosocial factors that improve job performance. The primary purpose of this study was to examine the impact of psychological empowerment, workplace motivation, and social capital on farmers’ job performance in Honduras.

A mixed methods approach was used to collect and analyze both quantitative and qualitative data. For quantitative data, a …

Simulating Southern Rust Damage In Corn Through Defoliation, Michael Keith Shannon

LSU Master's Theses

The importance of leaf area to corn for grain production beginning at silking is well documented. However, being able to predict yield loss due to defoliators such as foliar plant diseases and insects that progressively increase in defoliation over time has been difficult to quantify. To address this issue, a leaf removal study was conducted at the Dean Lee Research and Extension Center located near Alexandria, Louisiana in 2017 and 2018. Two hybrids, differing in relative maturity were evaluated in this study. An early maturing hybrid (108 days in 2017 and 107 days in 2018) and a later maturing hybrid …

Louisiana Beef Cattle Marketing Strategies, Adam Barrilleaux

LSU Master's Theses

The purpose of this study was to estimate the financial costs and returns of selected marketing strategies for cattle producers in the state of Louisiana. Cattle for the project were from the Louisiana State University Agricultural Center and commercial producers. Cattle were grouped by weight and sex and vaccinated, castrated, and handled using standard best management practices. The standard marketing practice for weaned calves in Louisiana of selling weaned animals at local stockyards was compared against alternative marketing of preconditioned calves through video auctions or after feedlot finishing. Feedlot cattle were sold both on live weight value and carcass weight …

The Effects Of Skeletal Muscle Specific Cpt1b Knock Out On Genetically Obese Ay Mice, Allison Stone

LSU Master's Theses

Fatty acid oxidation inhibition is one approach to reducing blood glucose levels in type II diabetes. Skeletal muscle specific Carnitine Palmitoyltransferase 1b knockout mice (Cpt1b^m-/-) cannot transport long-chain fatty acids into the mitochondria to be oxidized in order to produce energy. Cpt1b^m-/- mice have debilitated fat oxidation, less fat mass and improved glucose utilization compared to control C57BL/6 mice fed a 25% fat diet.

We hypothesized that CPT1b inhibition could reduce fat mass and lower blood glucose levels in a genetic mouse model of obesity and diabetes. To test this, we bred Cpt1b^m-/- mice to A …

Impacts Of Commercial Biopesticides On Crapemyrtle Bark Scale (Acanthococcus Lagerstroemiae) And Beneficial Insects, Giovana Matos Franco

LSU Master's Theses

The crapemyrtle bark scale (CMBS), Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is an important pest of crapemyrtles, Lagerstromia spp. (Myrtales: Lythraceae) since its damage results in an unpleasant aesthetic. Current CMBS management methods depend heavily on pesticides which impact on beneficial insects. Biopesticides show potential for pest control, host specificity, and low impact towards non-target organisms. The objectives of my thesis were to determine (1) if biopesticides are effective against CMBS infestations when applied in different seasons, and (2) their effects towards coccinellids known to attack CMBS.

To test the efficacy of selected biopesticides, treatments were delivered to potted plants or …

Larval Survival And Movement Of The Fall Armyworm And Corn Earworm (Lepidoptera: Noctuidae) In Seed Blends Of Non-Bt And Pyramided Bt Corn: Implications For Resistance Management, Marcelo Dimase

LSU Master's Theses

A seed blend refuge has been used in the U.S. Corn Belt for Bt corn insect resistance management (IRM). The fall armyworm, Spodoptera frugiperda (F.), and corn earworm, Helicoverpa zea (Boddie) are major target pests of Bt corn in the Americas. One major concern for the use of a seed blend refuge is that larval movement of insects among non-Bt and Bt plants could affect its efficacy for resistance management. To address this concern, field and greenhouse trials were conducted to determine 1) if seed blend refugia could provide comparable levels of susceptible S. frugiperda (aabb) as structured refugia and …

Spatial Distribution Of Lycorma Delicatula (Hemiptera: Fulgoridae) Egg Masses On Tree-Of-Heaven, Black Walnut, And Siberian Elm In North America, Houping Liu, Richard J. Hartlieb, Timothy Schowalter

Faculty Publications

The spatial distribution of Lycorma delicatula (White) egg masses on three species of trees were studied in Pennsylvania. Five tree-of-heaven, five black walnut, and one Siberian elm trees were felled in early spring 2019 to sample for egg masses. Each egg mass was marked for its cardinal direction, position in the tree, height above ground, and spread from the bole. A total of 214 egg masses were found on tree-of-heaven and black walnut, with 38.3, 29.4, 22.0, and 10.3% on the north, west, south, and east quadrant, respectively. No significant difference in cardinal direction was found for either species. Equal …

The Effects Of Oral And Venereal Transmission Of Deformed Wing Virus On Honey Bee (Apis Mellifera) Queen And Colony Health, Sarah Lang

LSU Master's Theses

Honey bees are important pollinators necessary for the production of many foods. Managed honey bee colonies have been experiencing high levels of colony loss over the last decade due to a combination of different factors. Parasites, pathogens, and queen failure are repeatedly reported as major causes for colony loss, but there is little research exploring the relationship between honey bee viruses and queens. Deformed wing virus, a major honey bee virus, has a worldwide distribution, is extremely prevalent, and can infect all castes and life stages. Honey bee queens can be infected with the virus through multiple transmission routes …