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Development, Characterization, And Technical Applications Of An Atlantic Killifish Ahr-2 Specific Monoclonal Antibody (Mab 5b6), Josephine Wojdylo Dec 2010

Development, Characterization, And Technical Applications Of An Atlantic Killifish Ahr-2 Specific Monoclonal Antibody (Mab 5b6), Josephine Wojdylo

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The aryl hydrocarbon receptor (AhR) functions as a ligand-activated transcription factor normally found in the cytoplasmic compartment of cells held by chaperones and immunophilin-like proteins. Ligand binding dissociates the AhR/ligand from chaperone proteins, allowing translocation to the nucleus with subsequent transcription of a suite of responsive genes, most notably Phase I, II, and III drug metabolism genes. Select environmental contaminants such as co-planar PCBs, planar polyaromatic hydrocarbons (PAHs), and halogenated aromatic hydrocarbons (HAHs) are potent AhR agonists, with 2,3,7,8 -tetrachlorodibenzodioxin (TCDD) being one of the most potent. Adverse effects of exposure to these potent environmental AhR ligands include immune suppression, …

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A Re-Examination Of Diplectrona Modesta Banks 1908 (Trichoptera: Hydropsychidae) Using Morphological And Molecular Techniques, Lauren Harvey Dec 2010

A Re-Examination Of Diplectrona Modesta Banks 1908 (Trichoptera: Hydropsychidae) Using Morphological And Molecular Techniques, Lauren Harvey

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Diplectrona modesta Banks 1908 (Trichoptera: Hydropsychidae) is a common inhabitant of streams across eastern North America. This species is well known and used often in biomonitoring studies. Experts in the field have long noted the morphological variation in larval head color and patterns as well as the species presence in many different types of habitats, such as springs, seeps, streams, and some small rivers. This study examines the species both morphologically and molecularly using mitochondrial and nuclear genes in order to determine if the species is a complex of several species or one highly variable species. DNA sequencing using the …

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Use Of Sub-Saharan Vultures In Traditional Medicine And Conservation And Policy Issues For The African Grey Parrot (Psittacus Erithacus), Kristina Dunn Dec 2010

Use Of Sub-Saharan Vultures In Traditional Medicine And Conservation And Policy Issues For The African Grey Parrot (Psittacus Erithacus), Kristina Dunn

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ABSTRACT
Wildlife populations worldwide are being negatively affected by the illegal wildlife trade. The severity of the impact to both Sub-Saharan vultures and African Grey Parrot (Psittacus erithacus) (AGP) populations are explored in this thesis. Many species of Sub-Saharan vultures are used in the traditional medicinal trade. Previous studies have found that vultures have mystical powers attributed to them due to their keen
ability to find food. AGP's are sought after for international trade due to their ability to mimic human vocabularies and for their aesthetic beauty.
Due to the illegal, secret, and illusive nature of this trade, the monetary …

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Letting The Light In, Owen Riley Dec 2010

Letting The Light In, Owen Riley

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LETTING THE LIGHT IN
Owen Riley Jr.
December 2010
ABSTRACT
'Forget your perfect offering, there is a crack in everything. That's how the light gets in.' Leonard Cohen
My thesis is a visual meditation on the transitions of life, death, love and emotion that come with age and responsibility. It is what I can touch and feel viewed through the frame of domesticity. It is a ballad of introspection, melancholy and hope redefining the beauty that has been masked by the fractures of time.
My thesis presents my self-created visions of the reality that surrounds me channeled through the 19th. …

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Chemical Composition And Antioxidant Properties Of Maya Nut (Brosimum Alicastrum), Hatice Kubra Tokpunar Dec 2010

Chemical Composition And Antioxidant Properties Of Maya Nut (Brosimum Alicastrum), Hatice Kubra Tokpunar

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ABSTRACT
Maya nut was investigated in term of its moisture, ash content, antioxidant activities, phenolic components by high pressure liquid chromatography-ultraviolet detector (HPLC-UV) and fatty acid composition by gas chromatography-flame ionization detector (GC-FID). The results showed that Maya nut was mainly composed of 55.06% linoleic acid (C18:2 n6), 27.2% palmitic acid (C16:0), 6.26% stearic acid (C18:0), 9.9% linolenic acid (C18:3 n3), and 3.36% Cis-11-Eicosenoic (C20:1). Maya nut possesses a higher content of polyunsaturated fatty acids (PUFAs) (linoleic and linolenic acid), but lower content of monounsaturated fatty acids (MUFAs) than canola oil and olive oil. The antioxidant capacities and total phenolic …

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In Vitro Boosting And Expansion Of Tumor Infiltrating Killer T Cells, Chunlei Mei Dec 2010

In Vitro Boosting And Expansion Of Tumor Infiltrating Killer T Cells, Chunlei Mei

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Immune cell tumor infiltration represents one of the mechanisms that the immune system responds to tumor cells. The tumor infiltrated immune cells include lymphocytes (TIL), dendritic cells (DCs), macrophages, and natural killer (NK) cells. Although TILs have been extensively studied in order to develop adoptive transfer based immunotherapies, how to effectively isolate, culture, and in vitro boosting the killer cells from TILs remains a challenge. Meanwhile, OK-432, a heat and penicillin-treated lyophilized preparation of the low-virulence strain of Streptococcus pyogenes, has been shown to exhibit immunomodulatory activities and potential antitumor therapeutic function, including the activation of DCs, neutrophils, macrophages, lymphocytes, …

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The Synthesis, Characterization, And Electrochemical Study Of Tris(Pyrazolyl)-Type Iron(Ii) Complexes, Iron(Ii) Sulfur- And Selenium-Containing Complexes, And Tris(Pyrazolyl)-Type Ruthenium(Ii) Complexes, Christopher Underwood Dec 2010

The Synthesis, Characterization, And Electrochemical Study Of Tris(Pyrazolyl)-Type Iron(Ii) Complexes, Iron(Ii) Sulfur- And Selenium-Containing Complexes, And Tris(Pyrazolyl)-Type Ruthenium(Ii) Complexes, Christopher Underwood

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Highly reactive radical species such as hydroxyl radical cause oxidative stress, resulting in chronic or degenerative diseases. In the biological Fenton reaction, iron(II) generates hydroxyl radical, OH, but only within a specific electrochemical potential window (-324 mV to 640 mV). Selenium and sulfur compounds have been extensively studied for their antioxidant properties, and they may exert their effects by binding iron(II) and shifting its electrochemical potential out of this window. This work has investigated the synthesis and electrochemical characterization of iron(II) chalcogenate complexes and iron(II) chalcogenone complexes. Cyclic voltammetry (CV) of [Fe(EPh)4][(PPh4)2] (E = S, Se) complexes shows Fe2+/3+ redox …

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Femoral Loading Mechanics In Virginia Opossums (Didelphis Virginiana): Torsion And Mediolateral Bending In Mammalian Parasagittal Locomotion, William Gosnell Dec 2010

Femoral Loading Mechanics In Virginia Opossums (Didelphis Virginiana): Torsion And Mediolateral Bending In Mammalian Parasagittal Locomotion, William Gosnell

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Studies of limb bone loading in terrestrial mammals have typically found anteroposterior bending to be the primary loading regime, with torsion contributing minimally. However, previous studies have focused on large, cursorial eutherian species in which the limbs are held essentially upright. Recent in vivo strain data from the Virginia opossum Didelphis virginiana, a marsupial that uses a crouched rather than upright limb posture, have indicated that its femur experiences moderate torsion during locomotion as well as strong mediolateral bending. The elevated femoral torsion and strong mediolateral bending observed in opossums (compared to other mammals) might result from external forces such …

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Characterization Of Transgenic Plum Lines Expressing Gastrodia Antifungal Protein (Gafp)-1, Hetalben Kalariya Dec 2010

Characterization Of Transgenic Plum Lines Expressing Gastrodia Antifungal Protein (Gafp)-1, Hetalben Kalariya

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The Gastrodia antifungal protein (GAFP-1) is a mannose-binding lectin originating from the Asiatic orchid Gastrodia elata. It has potential for conferring resistance to fungal and non-fungal pathogens in other plants which is currently being investigated. The goals of this research project were to determine (i) the potential movement of GAFP-1 protein from transgenic rootstocks into the non-transgenic scion of chimeric-grafted trees (ii) the levels of GAFP-1 protein in lines of the cultivar Bluebyrd expressing the gene gafp-1 under the control of the polyubiquitin promoter bul409, and (iii) the susceptibility of selected lines to the root pathogens Phytophthora cinnamomi Rands and …

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Isolation, Characterization And Application Of Bacteriophage To Treat Hydrogen Sulfide Producing Bacteria In Raw Animal Materials Destined For The Rendering Process, Chao Gong Dec 2010

Isolation, Characterization And Application Of Bacteriophage To Treat Hydrogen Sulfide Producing Bacteria In Raw Animal Materials Destined For The Rendering Process, Chao Gong

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In the United States, billions of pounds of animal by-products are generated by the food processing industry every year. Hydrogen sulfide producing bacteria (SPB) can utilize the sulfur-containing proteins and amino acids in the raw animal materials destined for the rendering process to produce harmful hydrogen sulfide (H2S) gas rapidly under the ambient conditions, resulting in hazardous working environments and inferior quality of finished products. In this study, the application of bacteriophage was explored as an effective solution for the elimination of H2S production in the rendering industry. The objectives of this study were to: 1) isolate and characterize strains …

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Experiencing Transition, Kara Renfro Dec 2010

Experiencing Transition, Kara Renfro

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ABSTRACT
My sculpture offers visual cues and displayed experiences for the viewer to reflect
upon past experiences and recognize the transitions that have occurred throughout their
lives because of those memories or adventures that become important due to their fragile and brief nature. These transitions from one place to another shed light on our past and unfold a journey that leads us through the inevitably constant changing circumstances of everyday life.
The artifacts and documents displayed throughout this body of work speak to the
human need to memorialize the past through collecting, documenting, and performing. It
is through observing this …

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Identification And Characterization Of Micrornas In Porcine Gametes And Pre-Implantation Embryos, Erin Curry Dec 2010

Identification And Characterization Of Micrornas In Porcine Gametes And Pre-Implantation Embryos, Erin Curry

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MicroRNAs (miRNAs) are short ribonucleic acids that ultimately affect the production of proteins. Although miRNAs are involved in nearly every biological process examined to date, little is known of the identity or function of miRNA in porcine reproductive tissues or their potential involvement in reproductive processes in pigs or other species. The objective of this dissertation research was to determine the presence of miRNAs in porcine gametes and both in vivo- and in vitro- produced pre-implantation embryos and to identify differences in miRNA expression between normal and aberrant samples. Using a heterologous RT-PCR approach, we demonstrated the presence of a …

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Deadly Discourse: Negotiating Bureaucratic Consensus For The Final Solution Through Organizational And Technical Communication, Mark Ward Sr Dec 2010

Deadly Discourse: Negotiating Bureaucratic Consensus For The Final Solution Through Organizational And Technical Communication, Mark Ward Sr

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The Final Solution was largely accomplished in eleven months; its executors, the Nazi SS, faced the constant problem that as killing and plunder escalated so did internal competition and corruption; and the SS deliberately cultivated an intensely competitive and polycratic organizational culture that fit the Nazi worldview of life-as-struggle. By tying these three observations together—that the Final Solution was punctuated, entropic, and polycratic—the problem arises: How did SS organizational communications manage, just barely long enough, to create a temporary social reality that regulated the internal contradictions of its genocidal project and fragmented bureaucracy? This study contends that through its organizational …

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A Study Of African American Forest Landowners In South Carolina: Implications For Land Ethic And Forest Stewardship, Keenan Adams Dec 2010

A Study Of African American Forest Landowners In South Carolina: Implications For Land Ethic And Forest Stewardship, Keenan Adams

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African Americans account for a significant proportion of South Carolina`s
population but reportedly own a disproportionally smaller amount of land. Research indicates that landowners who hold significant sources of sustainable wealth and power can dramatically increase their quality of life and ultimately that of their communities. Current land loss trends for African Americans are increasing at an alarming rate, which may have larger social implications for this traditionally underserved population. The remaining African American landowners are a rapidly declining group, due to an assortment of factors (e.g., age, heirs property). Understanding the experiences of the residual African American forest landowners …

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A Structural Equation Model Of Leader Attributes In The Principalship, Amy Young Dec 2010

A Structural Equation Model Of Leader Attributes In The Principalship, Amy Young

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To become a principal in South Carolina, one must have teaching experience, a master's degree, and certification in the area of primary responsibility. Beyond what is learned through coursework, internships, and certification tests, what intangible factors critically influence a principal's actions and decisions, and (ultimately) success? Trait theory begs the question of whether any of these criteria truly influences the decisions and actions of the principalship. Are there interactive traits of leadership that describe the behaviors of school principals? This study revisits trait theory by examining leader attributes relative to the principalship of South Carolina public schools. This study replicates, …

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Novel Vascular Grafts Based On Polyphenol-Stabilized Acellular Tissue Scaffolds, Ting-Hsien Chuang Dec 2010

Novel Vascular Grafts Based On Polyphenol-Stabilized Acellular Tissue Scaffolds, Ting-Hsien Chuang

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The Cardiovascular diseases (CVDs) continue to be the leading cause of morbidity and mortality worldwide. The most common form of CVDs is occlusion of blood flow thus limiting blood supply to specific tissues or organs. Atherosclerosis is a leading cause of coronary heart disease and stroke, which were responsible for more than 25% of deaths in 2004.
The demand for vascular graft is huge. In United States alone, approximately 500,000 coronary artery bypass graft surgeries are performed annually. Synthetic polymers such as Dacron and ePTFE have been successfully applied in large diameter blood vessel prosthesis; however, for small diameter (inner …

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Evaluation Of Factors That Modulate Cellular Adipogenesis For Breast Tissue Engineering Strategies, Cheryl Gomillion Dec 2010

Evaluation Of Factors That Modulate Cellular Adipogenesis For Breast Tissue Engineering Strategies, Cheryl Gomillion

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It is estimated that this year more than 200,000 women in the United States will be diagnosed with breast cancer. Treatment for most occurrences of breast cancer will often include surgical removal of the tumorigenic tissue, resulting in a soft tissue defect within the subcutaneous tissue of the skin. Post-surgical reconstruction methods are often sought by patients to restore the aesthetic function of the breast via cellular or non-cellular methods; however, because of complications associated with currently used methods for breast reconstruction, researchers are investigating tissue engineering methods to produce viable autologous adipose tissue for breast reconstruction.
Previous research in …

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Development And Characterization Of Novel Sub-Coatings With Polymorphic Brookite Titania Nanoparticles: Enhanced Uv/Vis Photocatalytic Antibacterial And Anticancer Properties, Donna Weinbrenner Dec 2010

Development And Characterization Of Novel Sub-Coatings With Polymorphic Brookite Titania Nanoparticles: Enhanced Uv/Vis Photocatalytic Antibacterial And Anticancer Properties, Donna Weinbrenner

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ABSTRACT
Nosocomial and community infections and biofilm formation from bacteria has increased significantly through adaptation combined with overuse of broad spectrum antibiotics. Because the world population continues to escalate, hospitals and long-term managed care will also escalate, thereby increasing transmission of infections, lowering patients' quality of health. Another disease on the rise throughout the world is skin cancer. A treatment modality that would cause less deleterious effects on the patient would be ideal. These two seemingly different issues could be solved with one product. First an inexpensive, safe, and non-selective antimicrobial surface coating would enhance the hospital arena and a …

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Sequencing And Functional Analysis Of A Multi-Component Dioxygenase From Pah-Degrading Sphingomonas Paucimobilis Epa505, Renuka Miller Dec 2010

Sequencing And Functional Analysis Of A Multi-Component Dioxygenase From Pah-Degrading Sphingomonas Paucimobilis Epa505, Renuka Miller

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Polycyclic aromatic hydrocarbons (PAHs) are hydrophobic organic compounds consisting of two or more fused benzene rings. PAHs derive from many different sources including petroleum refining, wood treatment, and coal coking industries. Because of their structural stability and water insolubility, PAHs are extremely resistant to degradation. These compounds are also believed to have mutagenic, carcinogenic, and teratogenic effects. Therefore, there are currently 16 PAH compounds on the EPA's list of priority pollutants.
Many species of bacteria have the ability to breakdown these persistent pollutants. However, bioremediation strategies using these organisms have many unresolved issues. While laboratory experiments can easily demonstrate the …

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Characterization Of Anti-Proteolytic And Anti-Proliferative Activities Of Pentagalloylglucose; Its Potential Application As A Therapeutic Agent In Vascular Diseases, Chaitra Cheluvaraju Dec 2010

Characterization Of Anti-Proteolytic And Anti-Proliferative Activities Of Pentagalloylglucose; Its Potential Application As A Therapeutic Agent In Vascular Diseases, Chaitra Cheluvaraju

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Cardiovascular diseases are the leading causes of mortality in the United States and will cost around $500 billion this year alone. Elevated proteolytic activity, increased proliferation and migration of vascular smooth muscle cells are hallmarks of atherosclerosis, stenosis and aortic aneurysms. These diseases often manifest the transdifferentiation of vascular smooth muscle cells into osteoblast-like cells followed by deposition of hydroxyapatite–like mineral in the arterial walls.
Currently, there are no standard treatments available for vascular calcification or aneurysms. Atherosclerosis treatment options are limited to statins while balloon angioplasty and stenting – surgical procedures for stenosis, often end in restenosis. Therefore, we …

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Breaking The Ice: Prospects For Canadian-American Institutional Change In The Governance Of The Northwest Passage, Jeffrey Parkey Dec 2010

Breaking The Ice: Prospects For Canadian-American Institutional Change In The Governance Of The Northwest Passage, Jeffrey Parkey

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This study assesses four institutional approaches to governing the use of the Northwest Passage, including the current rules in use. The assessment is conducted through the use of expert interviews, a review of the theoretical literature, and an examination of comparative cases. Because of significant environmental changes underway in the Arctic region, institutional change for Northwest Passage management is receiving increased attention. Due to the potential environmental and security impacts of regularized ship transits through the Northwest Passage, a number of informed observers have discussed the need for considering alternative means of governing the waterway. The advantages and disadvantages of …

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Comprehensive Thermal Modeling Of Power Split Hybrid Power-Train And Electronics, Abdel Mayyas Dec 2010

Comprehensive Thermal Modeling Of Power Split Hybrid Power-Train And Electronics, Abdel Mayyas

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Hybrid electric vehicle (HEV) uses both internal combustion engine (ICE) with an electric system. The combination of the electric power train with the ICE is intended to achieve both better fuel economies than the conventional vehicles and better performance. Several types of HEV exist with different layouts. Recent HEVs' make use of regenerative braking, which converts the vehicles' kinetic energy into electric energy instead of wasting it as heat as conventional brakes do. A hybrid-electric is more fuel efficient than ICE and has less environmental impact.
The new HEV with its new Key Characteristics and Configurations (i.e. Mechanical complexity, Multiple …

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Modulated Properties Of Fully Absorbable Bicomponent Meshes, Shawn Peniston Dec 2010

Modulated Properties Of Fully Absorbable Bicomponent Meshes, Shawn Peniston

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Current meshes used for soft-tissue repair are mostly composed of single component, nonabsorbable yarn constructions, limiting the ability to modulate their properties. This situation has left the majority of soft tissue repair load-bearing applications to suffer distinctly from undesirable features associated, in part, with mesh inability to (1) possess short-term stiffness to facilitate tissue stability during the development of wound strength; (2) gradually transfer the perceived mechanical load as the wound builds mechanical integrity; and (3) provide compliance with load transfer to the remodeling and maturing mesh/tissue complex. The likelihood of long-term complications is reduced for fully absorbable systems with …

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Successional Vegetation In The Jocassee Gorges, South Carolina, Maxwell Boyle Dec 2010

Successional Vegetation In The Jocassee Gorges, South Carolina, Maxwell Boyle

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Multifactor ecosystem classification systems provide a three-pronged approach to identifying site units across the landscape based on repeating patterns of vegetation, soil, and geomorphology. Ecosystem classification models have been developed for a diversity of forest landscapes throughout North America, and are beneficial as an ecosystem management tool because the outcome yields data models that can be utilized by scientists and natural resource managers alike. In contrast to the enormous amount of classification studies undertaken in relatively stable, older-aged forests in eastern North America, there have been few studies that have employed multifactor classification techniques across a successional gradient, or heavily …

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Modeling Microbial And Nutrient Dynamics In Zero-Discharge Aquaculture Systems, Kendall Kirk Dec 2010

Modeling Microbial And Nutrient Dynamics In Zero-Discharge Aquaculture Systems, Kendall Kirk

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A finite element, finite difference model was developed to predict nutrient and microbial dynamics in recirculating aquaculture systems. Total ammonia nitrogen, nitrite, nitrate, chemical oxygen demand, and dissolved oxygen concentrations are predicted in addition to growth and decay of algae, Nitrosomonas, Nitrobacter, denitrifying heterotrophs, and aerobic heterotrophs. Inputs to the model include temperature, photosynthetically available radiation, feed application rate, pH, aeration/mixing energy, water exchange configuration, water exchange rate, tilapia biomass, and dissolved oxygen concentration. Up to 10 exchanged units may be included, user-definable as continuous stirred tank reactors or sequencing batch reactors. Demonstration of the model's utility was demonstrated through …

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Preparation And Studies Of Fluorescent Carbon Nanomaterials, Xin Wang Dec 2010

Preparation And Studies Of Fluorescent Carbon Nanomaterials, Xin Wang

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There have been rapid advances in the development and applications of semiconductor quantum dots (QDs) represented by CdSe/ZnS. However, a serious limitation of these QDs is the necessary use of toxic heavy metals. Fluorescent 'carbon dots' (surface passivated carbon nanoparticles) are developed as alternative to classical semiconductor QDs.
The carbon dots could be made to be highly fluorescence, with emission quantum yields close to 60%. Their optical properties resemble bandgap transitions found in nanoscale semiconductors, suggesting that carbon particles at the nanoscale acquire essentially semiconductor-like characteristics.
The fluorescence in carbon dots could be quenched efficiently by electron acceptor or donor …

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The Tiger Vol. 104 Issue 24 2010-11-19, Clemson University Nov 2010

The Tiger Vol. 104 Issue 24 2010-11-19, Clemson University

Tiger Newspapers

No abstract provided.

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The Tiger Vol. 104 Issue 23 2010-11-12, Clemson University Nov 2010

The Tiger Vol. 104 Issue 23 2010-11-12, Clemson University

Tiger Newspapers

No abstract provided.

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The Impact Of A Youth Development Program On Secondary Students’ Career Aspirations, Bonita Williams, Jody Thompson, Tonya Taylor, Karen Eley Sanders Nov 2010

The Impact Of A Youth Development Program On Secondary Students’ Career Aspirations, Bonita Williams, Jody Thompson, Tonya Taylor, Karen Eley Sanders

Journal of Youth Development

This study’s purpose determined the extent to which adolescents’ participation in a youth development program may be linked to the participants’ post-secondary education and career aspirations. One hundred and seven adolescents, ages 14-19 in grades 8-12 completed Holland’s Vocational Interest Survey and the 4-H Career Decision Survey. Ordinal regression analysis indicated participation in 4-H had a positive impact on career decisions for students who participated in 4-H for two years (p< .038) and six years (p< .001). Significant differences were apparent with 80% of the racial/ethnic groups surveyed concerning a college fair’s impact on career choice and college major determination.

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Skill Transfer From Sight Translation To Simultaneous Interpreting: A Case Study Of An Effective Teaching Technique, Zhongwei Song Nov 2010

Skill Transfer From Sight Translation To Simultaneous Interpreting: A Case Study Of An Effective Teaching Technique, Zhongwei Song

International Journal of Interpreter Education

In this article, a case study is presented that demonstrates the potential of a new sight translation (ST) teaching technique for simultaneous interpreting (SI) training. By using animated, time-controlled PowerPoint presentations instead of texts on paper, this method induces constraints such as time pressure and attention splitting, thus making ST essentially an on-line information processing activity, closely resembling SI. Apart from reviewing how to design the method, the author compares it with the two existing methods (i.e., ST with prior reading and ST without prior reading), makes some hypothetical analysis of its functionality in SI training, and discusses some preliminary …

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