Digital Commons Network^™

Peanut Butter Jelly: An Animated Short Film, Alexander Beaty

All Theses

Peanut Butter Jelly is an animated short film based on the concept of two species of Jellyfish clashing over a jar of peanut butter. The production spanned a year and involved the artistic effort of 12 Digital Production Arts graduate students as well as a support team of 10 students from various programs within the University.

Physicocehmical Properties And Pre-Exposure Processes Govern Gold Nanoparticle Accumulation In Freshwater Species, Austin Thomas Wray

All Dissertations

Monitoring the distribution and subsequent effects of nanoparticle (NP) contaminants in aquatic ecosystems will be pivotal to developing regulations that minimize their environmental footprint. Regulators are in a unique position to take a proactive role in shaping how we produce and consume nanomaterials as opposed to the reactive role they have had to adopt with other contaminants. Over the last few decades, researchers have made great strides in describing the fate, behavior, and toxicity of NPs in environmental systems. Recent initiatives have made the transition to scenarios with greater environmental relevance, yet important aspects of fate and behavior remain unexplored. …

Arsenic Targets Neural Plate Border Specifier Cells In P19 Cells, Christopher Mccoy

All Theses

Epidemiological studies have shown that arsenic exposure during early embryogenesis can cause reduced weight gain and neurological deficits later on in life. In addition, in vitro and in vivo studies have indicated that arsenic suppresses neurogenesis and myogenesis. The exact mechanism of how arsenic causes these undesired developmental outcomes is poorly understood, however both skeletal muscle and sensory neuron development require the Wnt/β-catenin signaling pathway to initiate the specific differentiation of precursor cells. We were interested in determining the target cell population of arsenic and its metabolites. Arsenic's metabolites were of interest because they have been shown to be more …

Underwater God Rays From A Custom Volume Renderer, Gowthaman Ilango

All Theses

Peanut Butter Jelly, directed by Alexander Beaty, is a 51 second computer-animated short film produced by Digital Production Arts. The plot focuses on a fight sequence between a pirate jelly fish and a flyboy jelly fish over a peanut butter jar. The production demanded a photo-realistic computer generated underwater environment, which lead to the need for a custom built volume renderer to render high quality god rays. This thesis illustrates the requirement for a customized volume renderer for the production, the algorithm, and the implementation of the renderer. It also describes a tool created for Maya 2012 which gives the …

Sustenance, Brenton Pafford

All Theses

Utilizing the ceramic process I create objects that facilitate experiences in the domestic space. Developing sculptural elements that evoke memory traces, and in heightening the everyday to an aesthetic, I place value on the overlooked, under-thought items that sustain existence. 'It is the realm of these submerged memory-traces that creative art moves, bringing them into the orbit of everyday life and making them available to the experience of others by formalizing and projecting them onto elements of the familiar world which can receive and transmit them.' - Rawson The objects I created for this exhibition are rooted in memory traces, …

The Effects Of Conjugated Linoleic Acid Supplementation On Lipopolysaccharide-Challenged Synoviocytes, Allison Millican

All Theses

Osteoarthritis is one of the leading causes of lameness in the athletic horse. Cases of equine osteoarthritis typically present with synovitis, or inflammation of the synovium. This inflammation in the synovium leads to the production of inflammatory cytokines and eicosanoids. Although these downstream products can be beneficial, overproduction can lead to degradation of cartilage and joint disease. Opportunities to help mitigate or decrease inflammation within the joint are of current interest to the equine industry. One method being investigated is nutritional supplementation. The anti-inflammatory effects demonstrated by CLA supplementation make it a potential prospect for utilization in mitigating joint inflammation. …

What Adds Up To Being: The Work Of Tanna Burchinal, Tanna L. Burchinal

All Theses

My practice takes form around embodied experience. I affect signifiers of the human body within the ordered grid, the scientific text, and the logic of the machine, to highlight the interdependencies of physical bodies and those social constructs that produce and influence identity. We are a part of these constructs that both extend and limit; we are enacting and interacting with them. I do not aim to eradicate these structures of power (without them, our identities are in chaos). Instead, I point out the pitfalls of these constructs that are perceived as unchanging, by making interaction and experience integral to …

Determining The Fate Of Non-O157 Shiga Toxin-Producing Escherichia Coli In Dairy Compost During Storage Using The Optimized Detection Method, Hongye Wang

All Theses

Shiga toxin-producing Escherichia coli (STEC), a member of Enterobacteriaceae family, has been recognized as emerging pathogens. Dairy compost is commonly applied to farmland as a soil amendment. Despite the agricultural benefit of manure-based soil amendment, the inadequately treated compost can contribute to fresh produce contamination on the farm. Moreover, the epidemiological results showed that the non-O157 STEC cases have surpassed those of E. coli O157. Therefore, it is critical to evaluate the behavior of non-O157 STEC strains in the dairy manure-based compost. The objectives of this study were to: 1) optimize a culturing method for detecting non-O157 STEC from dairy …

Microneedle Arrays For Injection Seeding Of Tissue Engineered Scaffolds, Katelyn Michelle Rye

All Theses

Tissue engineered constructs have proven to possess comparable mechanical and physical characteristics to autologous tissues making them suitable replacements fordiseased anatomy when autologous tissue transplants are unavailable. However, difficulty in reseeding the constructs with cells prevents the tissue-engineered scaffoldsfrom becoming an adequate, equivalent replacement for diseased tissues when compared to autologous transplants. Cells adhere to the outer surfaces of scaffolds but are incapable of penetrating the constructs to provide complete cellularization. If cells were able to populate the inner layers of the tissue, then they could likely diffuse into the intimal and external layers of the construct to create a …

Environmental Fx For Multiple Productions, Hugh Kinsey Iii

All Theses

This thesis presents methods for creating visually interesting environmental visual effects using Houdini. Four different types of environmental effects are shown: Dust trails, Waterfall mist, Snow, and Underwater dust. Each of these effects emulate real world phenomena, but also have the ability to be artistically driven to fit the visual artist’s need.

Ecological, Physiological, And Molecular Characterization Of Annual Bluegrass (Poa Annua L.) Herbicide Resistance And Its Control On Golf Courses, Robert Cross

All Dissertations

Annual bluegrass (Poa annua L.) is one of the most pervasive, adaptable, and variable plant species in the world and is the most problematic winter annual weed in managed turfgrass. Its prolific seedhead production regardless of mowing height, clumping growth habit, and lack of tolerance to stress reduces turfgrass aesthetic quality and playability on golf courses. Its ability to germinate almost year-round influences cultural practices and herbicide use. Herbicides are an integral part of a weed control program for all weeds that invade a desirable turfgrass stand. However, inappropriate use of herbicides results in herbicide resistance issues. In recent years, …

Pre-Service Mathematics Teachers' Knowledge Development And Belief Change Within A Technology-Enhanced Mathematics Course, Vecihi Zambak

All Dissertations

With the adoption of Common Core State Standards, mathematics teachers have been expected to emphasize conceptual understanding as much as procedural and computational fluency in their teaching. Development of a sound mathematical content knowledge during their teacher education could enable mathematics teachers to meet this expectation. The infusion of technology into the domain of mathematics education has also modified the nature of mathematical content knowledge. Regarding the expectations and changes in the nature of mathematical content knowledge, in this dissertation, I examined the influence of Geometer's Sketchpad (GSP) on pre-service middle grade mathematics teachers' Specialized Content Knowledge (SCK); how their …

Design, Analysis And Implementation Of An Experimental System To Harvest Energy From Atmospheric Temperature Variations Using Ethyl Chloride Filled Bellows, Gibran Ali

All Theses

The increase in global warming and the dwindling supplies of fossil fuels have shifted the focus from traditional to alternate sources of energy. This has resulted in a concerted effort towards finding new energy sources as well as better understanding traditional renewable energy sources such as wind and solar power. In addition to the shift in focus towards alternate energy, the last two decades have offered a dramatic rise in the use of digital technologies such as wireless sensor networks that require small but isolated power supplies. Energy harvesting, a method to gather energy from ambient sources including sunlight, vibrations, …

Dietary Behaviors And Nutrition Intervention Preferences Of Breast Cancer Survivors, Sherry A. Stokes

All Theses

Breast cancer is the most commonly diagnosed cancer among women and there are expected to be 1,665,540 new cases diagnosed in 2014. Advancements in early detection, have greatly improved treatment outcomes for the majority of these patients and the 5-year survival rate is 89.2%. The newly emerging field of survivorship care is an attempt to meet the unique needs of this expanding population. Research has demonstrated that an unhealthy body weight is an important modifiable risk factor for the recurrence of breast cancer. This study used an electronic version of the Block 2005 to collect dietary information on 30 breast …

Invisible Labor And The Preservation Of Dignity, Laken Bridges

All Theses

My art seeks to question the social value of labor. Throughout history, labor hierarchies influenced by social class and economic stigmas have informed how laborers are viewed in the United States. Physical jobs such as menial and domestic work are a common form of invisible labor that experience debasement and stereotyping. In my art, I use labor-based and ordinary objects as a metaphor for the worker, linking the value or disposability of the object to the societal value of labor. This critique of labor is enhanced by the manipulation of text, by the formal tools of scale and perspective, and …

Development Of A Translational, Tissue-Engineered Approach To Replacement Of Myocardial Infarct Scar Tissue, Jason Schulte

All Dissertations

Myocardial infarction (MI) affects nearly 600,000 individuals each year, and the resulting damage initiates a pathophysiological progression towards congestive heart failure (CHF). A shortage of donor organs precludes heart transplantation as a practical solution, and neither surgical intervention nor stem cell therapy have yielded consistent and sufficiently positive results in clinical investigation. To prevent CHF, prospective therapies must target the cause of the maladaptive cardiac remodeling which precedes it--the nonfunctional, dyskinetic infarct scar--and aim to replace it with functional cardiac muscle. Tissue engineering holds promise for the development of novel therapies to either halt or reverse post-MI cardiac remodeling. However, …

Targeted Delivery Of Anti-Inflammatory And Anti-Oxidant Drugs For Treatment Of Chronic Obstructive Pulmonary Diseases, Yun Xiang

All Dissertations

Chronic obstructive pulmonary disease (COPD) was found to be the fourth leading cause of death in 2004, but current treatments can only relieve the symptoms and improve the quality of life for the patients. Glucocorticoids have been broadly applied in other inflammatory conditions with promising therapeutic effects, but performed poorly in COPD. The phenomenon called glucocorticoid resistance is responsible for glucocorticoid insensitivity in COPD. Researchers have proven that elevated oxidative stress in the lung of a COPD patient is one of the main causes of blocking the function of glucocorticoids. We hypothesize that reducing the oxidative stress in the lung …

Design With Emotion: Improving Web Search Experience For Older Adults, Tamirat Abegaz

All Dissertations

Research indicates that older adults search for information all together about 15% less than younger adults prior to making decisions. Prior research findings associated such behavior mainly with age-related cognitive difficulties. However, recent studies indicate that emotion is linked to influence search decision quality. This research approaches questions about why older adults search less and how this search behavior could be improved. The research is motivated by the broader issues of older users' search behavior, while focusing on the emotional usability of search engine user interfaces. Therefore, this research attempts to accomplish the following three objectives: a) to explore the …

Development And Application Of Scalable Two-Dimensional High Performance Liquid Chromatography For Aromatic Selective Separations Of Biologically Active Phytochemicals From Oplopanax Horridus, Gregory Jones

All Dissertations

The high demand for more efficient purification processes with increased automation and throughput pushes the development of more advanced preparative, pilot, and process scale HPLC instrumentation that is capable of achieving higher purities in a shorter amount of time than are currently achieved using one dimensional separations. A preparative scale 2D HPLC system was designed and reduced to practice in order to demonstrate the capacity for scalability of on-line comprehensive 2D HPLC separations of basic compounds from a challenging natural product extract of Oplopanax horridus. The methodology and instrumentation design herein permits direct method transfer from analytical to preparative scale …

Essays In Public And Corporate Finance, Joseph Newhard

All Dissertations

Each of the three chapters of this dissertation makes a unique contribution to the fields of public finance or corporate finance. In chapter one I show that tax-price is increasing in workplace risk due to a positive wage-risk response that is observed in the labor supply price for hazardous industries. This result implies that, holding human capital constant, workers in more dangerous industries will demand a relatively smaller public sector. I test this with county-level data on fatality rates and support for the two major party candidates in the 2004 US Presidential election. Taking Republicans to represent the party of …

The Development And Characterization Of Tannic Acid Cross-Linked Collagen Scaffolds For The Prevention Of Breast Cancer Recurrence In Lumpectomy Patients, Beau Inskeep

All Dissertations

Breast cancer is the second most common cancer amongst women, second only to lung cancer. In 2014, it was estimated that there were approximately 295,000 new cases of breast cancer diagnosed in women, with approximately 40,000 deaths caused by breast cancer in the United States. It has been reported that a woman has a 12% chance of developing breast cancer in her lifetime and a 3% chance of dying from breast cancer. There are several treatment options available for breast cancer, including chemotherapy, radiation therapy, as well as two different forms of surgery, mastectomy and lumpectomy. However, none of these …

Determining Dna Damage Prevention Mechanisms For Multifunctional Selenium And Sulfur Antioxidants And The Dna-Damaging Capabilities Of Clotrimadozle And Pseudoephedrine-Derived Metal Complexes, Matthew Todd Zimmerman

All Dissertations

DNA damage by reactive oxygen species (ROS) is a cause of many chronic diseases. This work examines the ability of sulfur and selenium antioxidants to prevent oxidative DNA damage and the mechanisms for this activity. Although iron- and copper-generated hydroxyl radical are primary causes of damage under oxidative stress conditions, studies typically focus on ROS scavenging rather than antioxidant-metal binding as a mechanism for sulfur and selenium antioxidant behavior. Mass spectrometry studies of sulfur and selenoamino acids (Chapter 2) show that most form CuI and FeII complexes, regardless of their metal-mediated DNA damage prevention abilities. Because their electrochemical properties do …

The Potential Of Exogenous Fatty Acids In Breast Tissue Engineering, Richard Thomas Pace

All Dissertations

More than 200,000 women are diagnosed with breast cancer each year in the United States, where the majority will undergo a lumpectomy procedure and then radiotherapy as preventative treatment. Both artificial and tissue transplants may obscure the detection of cancer recurrence or limit the effectiveness of radiation treatment. Previous research in our laboratory has been performed to develop injectable lumpectomy void fillers. This body of work seeks to continue and further develop the technology of the void fillers and further investigate the potential of fatty acids in breast tissue engineering.

Literature suggests that lipid peroxidation products from free fatty acids …

Earth Abundant Thin Film Technology For Next Generation Photovoltaic Modules, Githin Alapatt

All Dissertations

With a cumulative generation capacity of over 100 GW, Photovoltaics (PV) technology is uniquely poised to become increasingly popular in the coming decades. Although, several breakthroughs have propelled PV technology, it accounts for only less than 1% of the energy produced worldwide. This aspect of the PV technology is primarily due to the somewhat high cost per watt, which is dependent on the efficiency of the PV cells as well as the cost of manufacturing and installing them. Currently, the efficiency of the PV conversion process is limited to about 25% for commercial terrestrial cells; improving this efficiency can increase …

Sustainable Heritage Tourism: A Tourist-Oriented Approach For Managing Petra Archaeological Park, Jordan, Mohammad Alazaizeh

All Dissertations

Although sustainability of heritage tourism has become a major concern from the perspectives of both academics and practitioners, the majority of research and studies have tended to understand the cultural and educational issues of heritage tourism rather than focusing on the application of sustainability in a practical context. To achieve sustainability in heritage tourism, heritage sites should be managed effectively in a way that ensures preservation of heritage resources and provides quality experiences to the tourists. Traditionally, heritage tourism planning and management relied on a top-down, professional-led approach that ignores the interests of different stakeholders (e.g., heritage tourists) in heritage …

Ahr-Related Activities In A Creosote-Adapted Population Of Adult Atlantic Killifish, Fundulus Heteroclitus, Two Decades Post-Epa Superfund Status, Josephine Wojdylo

All Dissertations

Atlantic killifish, Fundulus heteroclitus, have adapted to, or at least developed a level of resistance to creosote-based polycyclic aromatic hydrocarbons (PAHs) found at the Atlantic Wood (AW) superfund site in the southern branch of the Elizabeth River, VA USA. Historically, 90+% of the mummichogs at this site have hepatic lesions of varying severity, and 30+% of these fish have tumors of various origin. Many xenobiotics, including select PAHs found at the Atlantic Wood site, are ligands for the aryl hydrocarbon receptor (AhR), which is a promiscuous ligand-dependent transcription factor involved in developmental regulation, environmental sensing, and induction of a suite …

Allosteric Regulation Of Bacterial And Fungal Xylulose 5-Phosphate/ Fructose 6-Phosphate Phosphoketolases (Xfps), Katie Glenn

All Dissertations

Acetate is excreted as a metabolic end product in many microbes. Acetate production has primarily been studied in bacteria and archaea but is known to occur in eukaryotic organisms as well. For example, acetate is one of the most abundant metabolites excreted by the fungal pathogen Cryptococcus neoformans during cryptococcosis suggesting that acetate production may be important during pathogenesis. One possible pathway for acetate production in C. neoformans involves the enzymes xylulose 5-phosphate/ fructose 6-phosphate phosphoketolase (Xfp), which can generate acetyl phosphate from either fructose 6-phosphate (F6P) or xylulose 5-phosphate (X5P), and acetate kinase (Ack), which can then convert acetyl …

Biology And Control Of The Bed Bug Cimex Lectularius L., Kevin Hinson

All Dissertations

After vanishing from the public eye for more than 50 years, bed bugs have resurged to become one of the most widely discussed and heavily researched insect pests in the westernized world. Our inability to prevent and successfully treat infestations has been the driving force behind this wave of research. I addressed gaps in our understanding of bed bugs by examining behavioral and life history characteristics, as well as insecticide application responses. I showed that natural-based products are generally ineffective against bed bugs, particularly when used as a residual treatment. I also found that bed bugs may be killed through …

Development Of An In Vitro Three-Dimensional Breast Tissue Model To Decipher The Effects Of Stromal Heterogeneity On Normal And Cancerous Mammary Epithelial Cells, Erin Mccave

All Dissertations

A goal of breast cancer research is to develop a detailed understanding of the underlying mechanisms of disease progression and to generate approaches to improve early detection, monitoring, and treatment of breast cancerous lesions. Current breast cancer therapies are not successful for all cases as they were developed as a standard of care for 'average' breast cancer condition. To further current clinical success, the characteristics of each tumor mass must be considered. Such an individualized or personalized approach is incompatible with current understanding of 'average' breast cancer tumors and responses to treatments. The long-term objective of the present research is …

Phylogenomics And Historical Biogeography Of The Gooseneck Barnacle Pollicipes Elegans, Sergio Andres Marchant Rojas

All Dissertations

This dissertation explores the systematics, biogeography, and genomics of the gooseneck barnacle Pollicipes elegans, a marine crustacean of the tropical Eastern Pacific. In Chapter 1, I provide a broad framework for my research by introducing and focusing on the long-­‐standing debate of the mechanisms behind the latitudinal gradient in species diversity, which provided the initial motivation for using Pollicipes elegans as a model system to study the mechanisms leading to genetic differentiation and speciation in tropical regions. In Chapter 2, I examine the genetic structure, infer patterns of connectivity across the warm tropical waters of the eastern Pacific, and reconstruct …