Digital Commons Network^™

Circularized Synthetic Oligodeoxynucleotides Serve As Promoterless Rna Polymerase Iii Templates For Small Rna Generation In Human Cells, Christine I. Seidl, Lodoe Lama, Kevin Ryan

Publications and Research

Synthetic RNA formulations and viral vectors are the two main approaches for delivering small therapeutic RNA to human cells. Here we report findings supporting an alternative strategy in which an endogenous human RNA polymerase (RNAP) is harnessed to make RNA hairpin-containing small RNA from synthetic single-stranded DNA oligonucleotides. We report that circularizing a DNA template strand encoding a pre-microRNA hairpin mimic can trigger its circumtranscription by human RNAP III in vitro and in human cells. Sequence and secondary structure preferences that appear to promote productive transcription are described. The circular topology of the template is required for productive transcription, at …

Senate Executive Committee Minutes December 17, 2012, Bronx Community College Senate Executive Committee

BCC Governance Archives

Minutes for the meeting of the Senate Executive Committee on December 17, 2012.

Senate Executive Committee Minutes December 17, 2012, Bronx Community College Senate Executive Committee

BCC Governance Archives

Minutes for the meeting of the Senate Executive Committee on December 17, 2013.

A Yeast Gsk-3 Kinase Mck1 Promotes Cdc6 Degradation To Inhibit Dna Re-Replication, Amy E. Ikui, Valentina Rossio, Lea Schroeder, Satoshi Yoshida

Publications and Research

Cdc6p is an essential component of the pre-replicative complex (pre-RC), which binds to DNA replication origins to promote initiation of DNA replication. Only once per cell cycle does DNA replication take place. After initiation, the pre-RC components are disassembled in order to prevent re-replication. It has been shown that the N-terminal region of Cdc6p is targeted for degradation after phosphorylation by Cyclin Dependent Kinase (CDK). Here we show that Mck1p, a yeast homologue of GSK-3 kinase, is also required for Cdc6 degradation through a distinct mechanism. Cdc6 is an unstable protein and is accumulated in the nucleus only during G1 …

A Study Of The Effect Of Heat-Treatment On The Morphology Of Nafion Ionomer Dispersion For Use In The Passive Direct Methanol Fuel Cell (Dmfc), Ting Yuan, Haifeng Zhang, Zhiqing Zou, Sufia Khatun, Daniel Akins, Yara Adam, Sophia Suarez

Publications and Research

Aggregation in heat-treated Nafion ionomer dispersion and 117 membrane are investigated by 1H and 19F Nuclear Magnetic Resonance (NMR) spectra, spin-lattice relaxation time, and self-diffusion coefficient measurements. Results demonstrate that heat-treatment affects the average Nafion particle size in aqueous dispersions. Measurements on heat-treated Nafion 117 membrane show changes in the 1H isotropic chemical shift and no significant changes in ionic conductivity. Scanning electron microscopy (SEM) analysis of prepared cathode catalyst layer containing the heat-treated dispersions reveals that the surface of the electrode with the catalyst ink that has been pretreated at ca. 80 °C exhibits a compact and uniform morphology. …

On The Social Construction Of Hellenism Cold War Narratives Of Modernity, Development And Democracy For Greece, Despina Lalaki

Publications and Research

Hellenism is one of those overarching, ever-changing narratives always subject to historical circumstances, intellectual fashions and political needs. Conversely, it is fraught with meaning and conditioning powers, enabling and constraining imagination and practical life. In this essay I tease out the hold that the idea of Hellas has had on post-war Greece and I explore the ways in which the American anti-communist rhetoric and discussions about political and economic stabilization appropriated and rearticulated Hellenism. Central to this history of transformations are the archaeologists; the archaeologists as intellectuals, as producers of culture who, while stepping in and out of their disciplinary …

What Can Phages Tell Us About Host-Pathogen Coevolution?, John J. Dennehy

Publications and Research

The outcomes of host-parasite interactions depend on the coevolutionary forces acting upon them, but because every host-parasite relation is enmeshed in a web of biotic and abiotic interactions across a heterogeneous landscape, host-parasite coevolution has proven difficult to study. Simple laboratory phage-bacteria microcosms can ameliorate this difficulty by allowing controlled, well-replicated experiments with a limited number of interactors. Genetic, population, and life history data obtained from these studies permit a closer examination of the fundamental correlates of host-parasite coevolution. In this paper, I describe the results of phage-bacteria coevolutionary studies and their implications for the study of host-parasite coevolution. Recent …

Lacuny Cataloging Roundtable Meeting Minutes, November 2012, Lacuny

Meeting Minutes

No abstract provided.

Naturalization Ceremonies And The Role Of Immigrants In The American Nation, Sofya Aptekar

Publications and Research

Although immigration is an essential element in the American national story, it presents difficulties for constructing national membership and national identity in terms of shared intrinsic values. In this article, I analyze speeches made at naturalization ceremonies during two time periods (1950 – 1970 and 2003 – present) to examine the evolving roles of immigrants, as articulated to immigrants themselves. Naturalization ceremonies are a unique research site because the usually implied nationalist content is made explicit to brand new members of the nation. I find a shift in the framing from immigrants as potential liabilities and weak links in the …

Combining Behavioral Weight Loss Treatment And A Commercial Program: A Randomized Clinical Trial, Angela Marinilli Pinto, Joseph L. Fava, Debra A. Hoffmann, Rena R. Wing

Publications and Research

Commercial weight loss programs such as Weight Watchers (WW) offer widely recognized and accessible treatment, but typically produce weight losses that are modest relative to professionally delivered programs. This study tested the hypothesis that a novel weight loss approach that combined the fundamental components of professionally delivered behavioral weight loss (BWL) treatment with the existing WW program would produce better weight losses than WW alone; no differences were expected between the novel treatment and BWL alone. Participants were 141 overweight and obese adults (90% women, 67% non-White, mean age = 49.7 ± 9.2 years, mean BMI = 36.2 ± 5.5 …

What Studios Do, Eliot Bates

Publications and Research

This essay is focused around a seemingly simple question – what do recording studios do? First, a clarification. I am not primarily asking “what are studios” or “what do people do in studios,” two comparatively straightforward questions that are tangentially addressed in academic and trade writing. Rather, I wish to consider some of the ways in which the studio itself shapes the kinds of social and musical performances and interactions that transpire within. I contend that studios must be understood simultaneously as acoustic environments, as meeting places, as container technologies, as a system of constraints on vision, sound and mobility, …

Intervening To Reduce Sedentary Behaviors And Childhood Obesity Among School-Age Youth: A Systematic Review Of Randomized Trials, May May Leung, Alen Agaronov, Kateryna Grytsenko, Ming-Chin Yeh

Publications and Research

Objective. To assess the effectiveness of interventions that focus on reducing sedentary behavior (SB) among school-age youth and to identify elements associated with interventions' potential for translation into practice settings. Methods. A comprehensive literature search was conducted using 4 databases for peer-reviewed studies published between 1980 and April 2011. Randomized trials, which lasted at least 12 weeks, aimed at decreasing SB among children aged 6 to 19 years were identified. Results. Twelve studies were included; 3 focused only on SB, 1 focused on physical activity (PA), 6 were combined SB and PA interventions, and 2 studies targeted SB, PA, and …

Teaching Information Literacy Online, Hal Grossman

Urban Library Journal

Book review

The New Distance Learners: Providing Customized Online Research Assistance To Urban Students On The Go, Melissa N. Mallon

Urban Library Journal

This article provides an overview of the pedagogical theories surrounding distance learning while discussing the learning styles and characteristics of distance learners. The author describes how the use of social media and learning objects supports the pedagogical theory of connectivism. The author discusses how a mid-sized, urban university library uses social media tools, including YouTube and Twitter, to offer distance learning students the same level of research support as those studying on campus while examining other libraries that do the same. This method provides students with the opportunity to pick and choose online research support that is customized to their …

Development Of A Ground Based Remote Sensing Approach For Direct Evaluation Of Aerosol-Cloud Interaction, Bomidi Lakshmi Madhavan, Yuzhe He, Yonghua Wu, Barry Gross, Fred Moshary, Samir Ahmed

Publications and Research

The possible interaction and modification of cloud properties due to aerosols is one of the most poorly understood mechanisms within climate studies, resulting in the most significant uncertainty as regards radiation budgeting. In this study, we explore direct ground based remote sensing methods to assess the Aerosol-Cloud Indirect Effect directly, as space-borne retrievals are not directly suitable for simultaneous aerosol/cloud retrievals. To illustrate some of these difficulties, a statistical assessment of existing multispectral imagers on geostationary (e.g., GOES)/Moderate Resolution Imaging Spectroradiometer (MODIS) satellite retrievals of the Cloud Droplet Effective Radius (Reff) showed significant biases especially at larger solar zenith angles, …

Government Information For Your Mobile Phone, Rebecca Arzola, Debbie Rabina

Publications and Research

A tasting menu of selected apps, created by U.S. government agencies, that provides information accessible from your mobile phone.

Classified Information: The Newsletter Of The Lloyd Sealy Library, Lloyd Sealy Library, Cuny John Jay College Of Criminal Justice

Reports and Publications from John Jay College

Classified Information was a publication that informed the College about Library services, personnel, upcoming events, new technologies, and available resources.

Students As Donors To University Archives: A Study Of Student Perceptions With Recommendations, Jessica Wagner, Debbi A. Smith

Publications and Research

Most of the literature that explores the relationship between students and university archives focuses on how to integrate archival materials into the undergraduate curriculum. Little exists that analyzes student perceptions of the types of materials that are found in archives; even less discusses how to induce students to donate materials that document life on campus. This case study examines student perceptions of various aspects of university archives, with the goal of developing better methods of reaching out to encourage donation of student life materials.

Competition Between Conjugation And M13 Phage Infection In Escherichia Coli In The Absence Of Selection Pressure: A Kinetic Study, Zhenmao Wan, Noel L. Goddard

Publications and Research

Inter- and intraspecies horizontal gene transfer enabled by bacterial secretion systems is a powerful mechanism for bacterial genome plasticity. The type IV secretion system of Escherichia coli, encoded by the F plasmid, enables cell-to-cell contact and subsequent DNA transfer known as conjugation. Conjugation is compromised by phage infection that specifically targets the secretion machinery. Hence, the use of phages to regulate the spread of genes, such as acquired antibiotic resistance or as general biosanitation agents, has gained interest. To predict the potential efficacy, the competition kinetics must first be understood. Using quantitative PCR to enumerate genomic loci in a resource-limited …

Multiculturalist Liberalism And Harms To Women: Lookin Through The Issue Of "The Veil", Anissa Helie, Marie Ashe

Publications and Research

Hélie & Ashe law review writing raises and responds to a reformulated and broadened version of Susan Okin’s 1999 inquiry, Is Multiculturalism Bad for Women? It identifies social and political developments, as well as legal and theoretical developments, that have occurred in the 21^st century and that demand that reformulation.

Not limiting itself (as did Okin’s question) to interrogating the relationship between women’s equality interests and interests in “religious freedom” advanced by minority-religious groups, Hélie & Ashe is the broader inquiry, critical for liberal theory of the 21^st century which has been greatly affected by the “ethos …

The Communicator, September 11, 2012, Bronx Community College

The Communicator

The Communicator newspaper published on September 11, 2012.

But Are They Connected?: A Report On The Queens College Technology Survey Of The Use Of Ubiquitous Tools For Learning, Eva M. Fernández, Michelle C. Fraboni

Publications and Research

The casual observer of the student population at Queens College (an urban, public, predominantly undergraduate campus) is awed by the ubiquity of technology, offering students the ability to communicate and participate in learning anytime, anywhere. They text, email, facebook, and tweet all day long. They have nearly instant access to the digital tools that have been touted as changing teaching and learning at all levels of education. With over 80% of our population born in or after 1980, there is an assumption that these digital natives intuitively know about and prefer digital tools for learning. With this in mind, and …

Catalog 2012-2013, Kingsborough Community College

Course Catalogs

No abstract provided.

Early Invasion Of Brain Parenchyma By African Trypanosomes, Ute Frevert, Alexandru Movila, Olga V. Nikolskaia, Jayne Raper, Zachary B. Mackey, Maha Abdulla, James Mckerrow, Dennis J. Grab

Publications and Research

Human African trypanosomiasis or sleeping sickness is a vector-borne parasitic disease that has a major impact on human health and welfare in sub-Saharan countries. Based mostly on data from animal models, it is currently thought that trypanosome entry into the brain occurs by initial infection of the choroid plexus and the circumventricular organs followed days to weeks later by entry into the brain parenchyma. However, Trypanosoma brucei bloodstream forms rapidly cross human brain microvascular endothelial cells in vitro and appear to be able to enter the murine brain without inflicting cerebral injury. Using a murine model and intravital brain imaging, …

The Structural Stability Of The Endothelial Glycocalyx After Enzymatic Removal Of Glycosaminoglycans, Ye Zeng, Eno E. Ebong, Bingmei M. Fu, John M. Tarbell

Publications and Research

Rationale It is widely believed that glycosaminoglycans (GAGs) and bound plasma proteins form an interconnected gel-like structure on the surface of endothelial cells (the endothelial glycocalyx layer–EGL) that is stabilized by the interaction of its components. However, the structural organization of GAGs and proteins and the contribution of individual components to the stability of the EGL are largely unknown. Objective To evaluate the hypothesis that the interconnected gel-like glycocalyx would collapse when individual GAG components were almost completely removed by a specific enzyme. Methods and Results Using confocal microscopy, we observed that the coverage and thickness of heparan sulfate (HS), …

Γ-Synuclein Interacts With Phospholipase Cβ2 To Modulate G Protein Activation, Urszula Golebiewska, Yuanjian Guo, Narindra Khalikaprasad, Cassandra Zurawsky, V. Siddhartha Yerramilli, Suzanne Scarlata

Publications and Research

Phospholipase Cβ2 (PLC β2) is activated by G proteins and generates calcium signals in cells. PLCβ2 is absent in normal breast tissue, but is highly expressed in breast tumors where its expression is correlated with the progression and migration of the tumor. This pattern of expression parallels the expression of the breast cancer specific gene protein 1 which is also known as γ-synuclein. The cellular function of γ-synuclein and the role it plays in proliferation are unknown. Here, we determined whether γ-synuclein can interact with PLCβ2 and affect its activity. Using co-immunprecitation and co-immunofluorescence, we find that in both benign …

Weaponizing Tear Gas: Bahrain’S Unprecedented Use Of Toxic Chemical Agents Against Civilians., Richard Sollom, Holly G. Atkinson

Publications and Research

The Bahraini government’s response to the early 2011 pro-democracy protests was brutal, systematic, and violent. In addition to birdshot and rubber bullets, government law enforcement attacked unarmed protestors with toxic chemical agents including tear gas. The government’s crackdown on the medical profession was especially harmful, as security forces arrested and detained doctors, raided health facilities, and obstructed patients from receiving necessary care. This report’s findings are based on field research that the authors conducted in Bahrain (April 2012) to investigate excessive use of force by law enforcement officials since June 2011—the end of Bahrain’s state of emergency. The medico-legal team …

Loss Of A 20s Proteasome Activator In Saccharomyces Cerevisiae Downregulates Genes Important For Genomic Integrity, Increases Dna Damage, And Selectively Sensitizes Cells To Agents With Diverse Mechanisms Of Action, Kevin M. Doherty, Leah D. Pride, James Lukose, Brian E. Snydsman, Ronald Charles, Ajay Pramanik, Eric G. Muller, David Botstein, Carol Wood Moore

Publications and Research

Cytoprotective functions of a 20S proteasome activator were investigated. Saccharomyces cerevisiae Blm10 and human 20S proteasome activator 200 (PA200) are homologs. Comparative genome-wide analyses of untreated diploid cells lacking Blm10 and growing at steady state at defined growth rates revealed downregulation of numerous genes required for accurate chromosome structure, assembly and repair, and upregulation of a specific subset of genes encoding protein-folding chaperones. Blm10 loss or truncation of the Ubp3/Blm3 deubiquitinating enzyme caused massive chromosomal damage and cell death in homozygous diploids after phleomycin treatments, indicating that Blm10 and Ubp3/Blm3 function to stabilize the genome and protect against cell death. …

Halogen Activation Via Interactions With Environmental Ice And Snow In The Polar Lower Troposphere And Other Regions, J. P. D. Abbatt, J. L. Thomas, K. Abrahamsson, Chris Boxe, A. Granfors, A. E. Jones, M. D. King, A. Saiz-Lopez, P. B. Shepson, J. Sodeau, D. W. Toohey, C. Toubin, R. Von Glasow, S. N. Wren, X. Yang

Publications and Research

The role of ice in the formation of chemically active halogens in the environment requires a full understanding because of its role in atmospheric chemistry, including controlling the regional atmospheric oxidizing capacity in specific situations. In particular, ice and snow are important for facilitating multiphase oxidative chemistry and as media upon which marine algae live. This paper reviews the nature of environmental ice substrates that participate in halogen chemistry, describes the reactions that occur on such substrates, presents the field evidence for ice-mediated halogen activation, summarizes our best understanding of ice-halogen activation mechanisms, and describes the current state of modeling …

Comparing Unique Title Coverage Of Web Of Science And Scopus In Earth And Atmospheric Sciences, Philip Barnett, Claudia Lascar

Publications and Research

No abstract provided.