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Microtubules In Breast Cancer: Exploring The Α/Β-Tubulin Toggle Switch And Its Implications In Human Breast Cancer, Annemarie Ianos

Student Theses and Dissertations

Microtubules, composed of a/b-tubulin heterodimers, play a central role in breast cancer tumor growth by polymerizing, leading to metastasis and depolymerizing, contributing to proliferation. Human enzymes protein kinase Ca (PKC-a) and cyclin-dependent kinase 1 (Cdk-1) mediate phosphorylation at sites a:Ser165 and b:Ser172, respectively, influencing the growth of microtubules. It is possible that alternating phosphorylation at these sites contribute to an a/b-tubulin “toggle switch” that mediates microtubule instability and tumor growth.

The project investigates the influence of the toggle switch model on microtubule stability by determining the impact of mutants (a:S165D, a:S165N, a:S165S_P, b:S172S_P and a:S165S_P/b:S172S …

Human Physiology And Exercise Physiology Syllabus, Jean-Philippe Berteau

Open Educational Resources

No abstract provided.

Vertex-Based Analysis Of Cerebral Blood Flow And Fractional Amplitude Of Low-Frequency Fluctuations (Cbf-Falff) Coupling In Moderate-To-Severe Traumatic Brain Injury During The First Year Post-Injury, Vaidehi Hemkumar Patel

Dissertations, Theses, and Capstone Projects

Traumatic brain injury (TBI) is known to involve damage in the neurovascular unit. TBI may disrupt the brain’s neurovascular coupling–the relationship between cerebral blood flow and neural activity–that can lead to cognitive and functional impairments. This study attempted to non-invasively investigate neurovascular coupling in patients with moderate to severe TBI (msTBI) during the first year post-injury using a combined metric of cerebral blood flow (CBF) and fractional amplitude of low-frequency fluctuations (fALFF), a proxy for spontaneous neural activity. Using arterial spin labeling (ASL) and functional MRI sequences, CBF and fALFF were measured in 29 msTBI patients 3-, 6- and 12-months …

Drosophila Parasitoids Go To Space: Unexpected Effects Of Spaceflight On Hosts And Their Parasitoids, Jennifer Chou, Johnny R. Ramroop, Amanda M. Saravia-Butler, Brian Wey, Matthew P. Lera, Medaya L. Torres, Mary Ellen Heavner, Janani Iyer, Siddhita D. Mhatre, Sharmila Bhattacharya, Shubha Govind

Publications and Research

While fruit flies (Drosophila melanogaster) and humans exhibit immune system dysfunction in space, studies examining their immune systems’ interactions with natural parasites in space are lacking. Drosophila parasitoid wasps modify blood cell function to suppress host immunity. In this study, naive and parasitized ground and space flies from a tumor-free control and a blood tumor-bearing mutant strain were examined. Inflammation-related genes were activated in space in both fly strains. Whereas control flies did not develop tumors, tumor burden increased in the space-returned tumor-bearing mutants. Surprisingly, control flies were more sensitive to spaceflight than mutant flies; many of their …

A Biological Lens On Food Deserts [Biology], Claudette Davis

Open Educational Resources

SCB101 is a non-major biology course. In Fall 2022, the course was designated as an Experiential Learning-LaGuardia Humanitarian Initiative (LHI) course in CUNYFirst. LHI is LaGuardia's experiential learning program that helps students to critically reflect on global issues and develop sustainable solutions in partnership with local and global organizations. For the 2022-2023 academic year, LHI focused on two United Nations Sustainable Development Goals (UNSDG): Goal 2 and Goal 10. UNSDG 2 aims to end hunger, achieve food security, improve nutrition, and promote sustainable agriculture. UNSDG 10 addresses reducing inequality both within and among countries. Thus, I developed an assignment to …

Introduction To Human Biology, Sharon S. Ellerton, Christopher Roblodowski

Open Educational Resources

This OER is intended as a textbook for a one semester introductory course in Human Anatomy and Physiology for non-science majors. It covers the major topics typically covered in A&P, but in a simplified, easier to understand manner. This textbook aims to educate students interested in lower-level health careers and non-science majors without the intimidating detail found in current textbooks. Text and images were created to be more accessible for these student populations.

Introduction To Psychology, Gail Hayes

Open Educational Resources

Introduction to Psychology is a modified version of Psychology 2e - OpenStax.

Comparison Of Various Intermittent Fasting Dietary Interventions To The Ketogenic Dietary Interventions And Its Effects In Mitigating Hunger In Obese Adults, Aston Earl Francis Jr

Theses and Dissertations

There have been many dietary strategies that can be used for fat loss and the most recent popular dietary interventions have been intermittent fasting and the ketogenic diet. Intermittent fasting (IF) dietary interventions and the ketogenic diet (KD) are popular diets that claim to promote weight loss which has also been speculated to have enhanced benefits than caloric restriction. Although these dietary strategies are growing in popularity, it is unclear if the intervention can manage hunger, which may increase adherence. The purpose of this paper is to systematically review the literature on the impact of IF protocols compared to KD …

Cerebrovascular Impairment As A Potential Target For Neuromodulation Therapy In Moderate-Severe Traumatic Brain Injury, Naomi L. Gaggi

Dissertations, Theses, and Capstone Projects

The main purpose of this dissertation is to characterize noninvasive neuroimaging biomarkers of neurovascular injury in moderate-severe traumatic brain injury (msTBI) as potential treatment targets for neuromodulation therapy. In Chapter 1, I aim to introduce msTBI and two known sequelae of neurovascular damage in msTBI: reduced cerebral blood flow (Ware et al., 2020; Julliene et al., 2016) and altered low frequency fluctuations in blood oxygen level-dependent signal (Zhou et al., 2021; Palacios et al., 2013). Chapter 2 aims to identify the regional pattern of altered resting cerebral blood flow from a cohort of 29 patients with msTBI assessed at 3, …

Physiological Processes, Chirag B. Raval

Open Educational Resources

Physiological Processes is designed to introduce fundamental concepts of physiology to biomedical engineering learners. Areas covered include: neural, muscular, cardiovascular, respiratory and renal system function; bioelectrical signals, capillary-level transport, organ-level exchange. Whenever possible, analysis will be quantitative in order to fulfill the course goals and to build the foundation for the subsequent biomedical engineering curriculum. This course is for Biomedical Engineering majors only.

Unthinkable Conditions: Affect And Environment In Romanticism And Speculative Fiction, Amelia Z. Greene

Dissertations, Theses, and Capstone Projects

Unthinkable Conditions bridges two literary periods and two theoretical modes in order to illustrate important parallels between historical periods and the writers who attempted to approach the changing environmental conditions of their respective eras. Each chapter names and theorizes a unique form of feeling which then serves as a framework for eco-affective analysis, drawing from existing studies in the environmental humanities and in studies of affect in order to construct a hybrid theoretical model which more fully accounts for the work of the writer treated in each chapter. The central claim of this dissertation is that vital affective innovations accompany …

No Ordinary Proteins: Adsorption And Molecular Orientation Of Monoclonal Antibodies, Ankit D. Kanthe, Andrew Ilott, Mary Krause, Songyan Zheng, Jinjiang Li, Wei Bu, Mrinal K. Bera, Binhua Lin, Charles Maldarelli, Raymond S. Tu

Publications and Research

The interaction of monoclonal antibodies (mAbs) with air/water interfaces plays a crucial role in their overall stability in solution. We aim to understand this behavior using pendant bubble measurements to track the dynamic tension reduction and x-ray reflectivity to obtain the electron density profiles (EDPs) at the surface. Native immunoglobulin G mAb is a rigid molecule with a flat, “Y” shape, and simulated EDPs are obtained by rotating a homology construct at the surface. Comparing simulations with experimental EDPs, we obtain surface orientation probability maps showing mAbs transition from flat-on Y-shape configurations to side-on or end-on configurations with increasing concentration. …

Anatomy And Physiology Preparatory Course Textbook (2nd Edition), Carlos Liachovitzky

Open Educational Resources

The goal of this preparatory textbook is to give students a chance to become familiar with some terms and some basic concepts they will find later on in the Anatomy and Physiology course, especially during the first few weeks of the course.

Organization and functioning of the human organism are generally presented starting from the simplest building blocks, and then moving into levels of increasing complexity. This textbook follows the same presentation. It begins introducing the concept of homeostasis, then covers the chemical level, and later on a basic introduction to cellular level, organ level, and organ system level. This …

Transit Mobility And Premature Birth In New York City: A Population-Based Study, Ross B. Maddux

Theses and Dissertations

Transit mobility has been recognized as an essential element to economic attainment, environmental sustainability, and as a vital part of public health⁠. Social and economic disparities paired with elusive and inefficient public transit present real barriers for the ability of efficient planning and use of time⁠. Lengthier commutes and generally less efficient travel contribute to stress during a pregnancy and limit the ability to access work and prenatal care. In survey studies of women utilizing clinics as their prenatal care provider, nearly 25% listed transportation as a barrier to accessing care.

Prior research has not linked directly premature birth and …

Teacher Wellbeing In A Juvenile Detention Facility, Elizabeth G. Baker

Dissertations, Theses, and Capstone Projects

“Teaching is inextricably linked to teachers’ personal lives; teachers invest their selves and their sense of identity and self-esteem in their work” (Rinchen, Ritchie, & Bellocchi, 2016, p. 604).

The teaching profession in the United States of America can be a highly stressful one, especially in America’s largest urban centers, due to the high incidences of violence, lack of resources and insufficient funding (Tobin, Roth & Zimmermann, 2001). The situation is even worse for teachers teaching in juvenile detention centers with traumatized youth and many teachers may internalize this trauma. Teachers may experience tremendous emotional tensions, such as …

Vulnerability: Sensation And Subjectivity In The Late Victorian Novel, Michael Shelichach

Dissertations, Theses, and Capstone Projects

“Vulnerability: Sensation and Subjectivity in the Late Victorian Novel” explores how developments in the science of psychology in the second half of the nineteenth century destabilized the genre of literary realism in Britain. Prominent mid-Victorian psychologists theorized a subject with a highly impressionable brain and nervous system. This new understanding of the mind’s potential vulnerability to external influence impelled contemporary novelists to contemplate the extent to which subjective interiority could be altered by the environment. Through close readings of canonical realist novels like Charles Dickens’s Great Expectations, George Eliot’s The Mill on the Floss and Wilkie Collins’s The Moonstone …

Human Health And Wellness In Space: A Review Of Common Effects, Current Research, And Methods Of Prevention, Sonnet Xu, Rajendra Persaud, Nazrul I. Khandaker, Jasmin Budhan

Publications and Research

Space poses a threat to space crew wellness, where microgravity, isolation, radiation, and other conditions exert strain on human health. This study explores the changes to the human body in space and how it contributes to major threats to health during spaceflights. The effects of the hostile environment of outer space are all examined here, along with resultant changes to behavior, physiology, and the difficulty of remote diagnosis for health treatment in space. This review also considers the implications to the human cartilage, brain, and eyes along with the various other hazards and physiological alterations.

Human health in space is …

Microgravity And Its Bearing With Space Flight-Related Research, Farhan Tanvir, Nazrul I. Khandaker, Rajendra Persaud, Jeff Guan

Publications and Research

This study investigates the unique properties and applications of microgravity in the context of spaceflight research. Understanding and harnessing the behaviors of a microgravity environment opens up the doors to long-term space travel and consequently, future missions to other planets within our solar system. More specifically, simulating and conducting experiments in microgravity environments helps us explore its effects on the various necessary tasks and behaviors that are carried out during spaceflight. The most immediate and pronounced effects of microgravity are on human physiology. In particular, studies have shown that prolonged exposure to microgravity can result in a 20% decrease in …

Raw Veganism: The Human Diet, Carlo Alvaro

Publications and Research

In this chapter, I present some compelling evidence that points to the conclusion that the human species has a specific diet, which is a raw vegan diet of fresh fruit, tender leafy greens, and a moderate amount of nuts and seeds. Out of the hundreds of thousands of species in the world, the only one—ours—cooks food. Not surprisingly, our species is the only one that suffers from numerous maladies. In other words, the argument is that the consumption of cooked food (especially animal-based) is unhealthful and undermines our nature. I present undeniable evidence, from evolutionary science to nutrition science, showing …

A Neural Mechanism For Capnotaxis In The Naked Mole-Rat, Michael Zions

Dissertations, Theses, and Capstone Projects

A Neural Mechanism for Capnotaxis in the Naked Mole-Rat

The Naked Mole-Rat (NM-R) is a small hairless rodent that has thrived underground in the Horn of Africa for the past 40 million years. It was first discovered in 1847, but has only been maintained in captivity and studied as a laboratory animal over the past 40 years. To date, more than 400 scientific papers have been published on this species. This is relatively few compared to established lab animals like rats and mice, but those papers contain a trove of information that has changed our understanding of the constraints of …

Hegemony Versus Pluralism: Ayurveda And The Movement For Global Mental Health, Murphy Halliburton

Publications and Research

Under the aegis of the World Health Organization, the Movement for Global Mental Health and an Indian Supreme Court ruling, biomedical psychiatric interventions have expanded in India augmenting biomedical hegemony in a place that is known for its variety of healing modalities. This is occurring despite the fact that studies by the WHO show a better outcome in India for people suffering schizophrenia and related diagnoses when compared to people in developed countries who have greater access to biomedical psychiatry. Practitioners of ayurvedic medicine in Kerala have been mounting a claim for a significant role in public mental health in …

Adapting To Extreme Heat: Social, Atmospheric, And Infrastructure Impacts Of Air Conditioning In Megacities - The Case Of New York City, Harold Gamarro

Dissertations and Theses

Extreme heat events are becoming more frequent and intense in most large cities. Built-up surfaces also limit cooling mechanisms, leading to warmer conditions in cities, a phenomenon called the Urban Heat Island (UHI). This presents major challenges to reduce adverse health effects of hot weather, particularly in vulnerable populations like the elderly and low-income communities. Here we explore the overall impacts of increasing air conditioning (AC) system adoption in residences as an adaptive measure to reduce human health risks under heat waves, with New York City (NYC) as a case study. This study uses AC adoption data from the 2017 …

Convergence Of Human And Old World Monkey Gut Microbiomes Demonstrates The Importance Of Human Ecology Over Phylogeny, Katherine R. Amato, Elizabeth K. Mallott, Daniel Mcdonald, Nathaniel J. Dominy, Tony Goldberg, Joanna E. Lambert, Larissa Swedell, Jessica L. Metcalf, Andres Gomez, Gillian A. O. Britton, Rebecca M. Stumpf, Steven R. Leigh, Rob Knight

Publications and Research

Background

Comparative data from non-human primates provide insight into the processes that shaped the evolution of the human gut microbiome and highlight microbiome traits that differentiate humans from other primates. Here, in an effort to improve our understanding of the human microbiome, we compare gut microbiome composition and functional potential in 14 populations of humans from ten nations and 18 species of wild, non-human primates.

Results

Contrary to expectations from host phylogenetics, we find that human gut microbiome composition and functional potential are more similar to those of cercopithecines, a subfamily of Old World monkey, particularly baboons, than to those …

Composite Biomaterial Repair Strategy To Restore Biomechanical Function And Reduce Herniation Risk In An Ex Vivo Large Animal Model Of Intervertebral Disc Herniation With Varying Injury Severity, Warren W. Hom, Melanie Tschopp, Huizi A. Lin, Philip Nasser, Damien M. Laudier, Andrew C. Hecht, Steven B. Nicoll, James C. Iatridis

Publications and Research

Back pain commonly arises from intervertebral disc (IVD) damage including annulus fibrosus (AF) defects and nucleus pulposus (NP) loss. Poor IVD healing motivates developing tissue engineering repair strategies. This study evaluated a composite injectable IVD biomaterial repair strategy using carboxymethylcellulose-methylcellulose (CMC-MC) and genipincrosslinked fibrin (FibGen) that mimic NP and AF properties, respectively. Bovine ex vivo caudal IVDs were evaluated in cyclic compression-tension, torsion, and compression-to-failure tests to determine IVD biomechanical properties, height loss, and herniation risk following experimentally-induced severe herniation injury and discectomy (4 mm biopsy defect with 20% NP removed). FibGen with and without CMC-MC had failure strength similar …

Development And Psychometric Analysis Of The Roy Adaptation Modes Scale (Rams) To Measure Coping And Adaptation, Sandra A. Russo

Dissertations, Theses, and Capstone Projects

Peoples’ lives are often interrupted or changed by experiencing a serious illness. It is important to study and understand the various coping processes that people have in adapting to illness. The purpose of this study was to develop an instrument to measure a person’s coping response to illness-related stimuli using the Roy Adaptation Model (RAM) as its structure and framework. This study is quantitative descriptive design to develop the validity and reliability of a new instrument the Roy Adaptation Modes Scale (RAMS). A review of RAM based measurement instruments found no instrument that measured all four adaptive modes that is …

Stabilizing Forces In Acoustic Cultural Evolution: Comparing Humans And Birds, Daniel C. Mann

Dissertations, Theses, and Capstone Projects

Learned acoustic communication systems, like birdsong and spoken human language, can be described from two seemingly contradictory perspectives. On one hand, learned acoustic communication systems can be remarkably consistent. Substantive and descriptive generalizations can be made which hold for a majority of populations within a species. On the other hand, learned acoustic communication systems are often highly variable. The degree of variation is often so great that few, if any, substantive generalizations hold for all populations in a species.

Within my dissertation, I explore the interplay of variation and uniformity in three vocal learning species: budgerigars (Melopsittacus undulatus), …

A Critical Evaluation Of The Biological Construct Skeletal Muscle Hypertrophy: Size Matters But So Does The Measurement, Cody T. Haun, Christopher G. Vann, Brandon M. Roberts, Andrew D. Vigotsky, Brad J. Schoenfeld, Michael D. Roberts

Publications and Research

Skeletal muscle is highly adaptable and has consistently been shown to morphologically respond to exercise training. Skeletal muscle growth during periods of resistance training has traditionally been referred to as skeletal muscle hypertrophy, and this manifests as increases in muscle mass, muscle thickness, muscle area, muscle volume, and muscle fiber cross-sectional area (fCSA). Delicate electron microscopy and biochemical techniques have also been used to demonstrate that resistance exercise promotes ultrastructural adaptations within muscle fibers. Decades of research in this area of exercise physiology have promulgated a widespread hypothetical model of training-induced skeletal muscle hypertrophy; specifically, fCSA increases are accompanied by …

Writing To Learn: Embedding A Crucial Study Skill In A Gateway Science Course, Shylaja Akkaraju

Publications and Research

Handwriting is a multisensory process known to enhance memory, focus, engagement, and cognition in the learner, thereby making it a valuable study skill. In an attempt to embed handwriting as a study skill in a gateway science course, I used a combination of scaffolding and individual student consultations. Scaffolding emphasized the use of desirable difficulties and retrieval practice through the implementation of techniques such as the flipped learning approach and take home-in class hybrid essay exams. While the former helped to increase overall student success the latter technique was aimed at enhancing written fluency in the subject, curbing procrastination, and …

Ligand Modulation Of Sidechain Dynamics In A Wild-Type Human Gpcr, Lindsay D. Clark, Igor Dikiy, Karen Chapman, Karin Ej Rodstrom, James Aramini, Michael V. Levine, George Khelashvili, Soren Gf Rasmussen, Kevin H. Gardner, Daniel M. Rosenbaum

Publications and Research

GPCRs regulate all aspects of human physiology, and biophysical studies have deepened our understanding of GPCR conformational regulation by different ligands. Yet there is no experimental evidence for how sidechain dynamics control allosteric transitions between GPCR conformations. To address this deficit, we generated samples of a wild-type GPCR (A2AR) that are deuterated apart from 1H/13C NMR probes at isoleucine d1 methyl groups, which facilitated 1H/13C methyl TROSY NMR measurements with opposing ligands. Our data indicate that low [Na+] is required to allow large agonist-induced structural changes in A2AR, and that patterns of sidechain dynamics substantially differ between agonist (NECA) and …

Pulling The Plug On Microscopes In The Anatomy And Physiology Laboratory, Zvi Ostrin, Vyacheslav Dushenkov

Publications and Research

Virtual microscopy (VM) has been widely available for more than a decade, especially in clinical settings and medical schools. In recent years the movement away from conventional optical microscopy (OM) and towards VM has been accelerating and several VM websites are now available online and readily accessible to educators. VM can enhance the instructor’s ability to teach the histology component of anatomy and physiology classes, facilitate student learning, save time for both students and instructors, and ultimately save money that can be more productively used for other facets of the laboratory. The many pedagogical and practical advantages provided by VM …