Digital Commons Network^™

Species-Level View Of Population Structure And Gene Flow For A Critically Endangered Primate (Varecia Variegata), Andrea L. Baden, Sheila M. Holmes, Steig E. Johnson, Shannon E. Engberg, Edward E. Louis, Brenda J. Bradley

Publications and Research

Lemurs are among the world's most threatened mammals. The critically endangered black-and-white ruffed lemur (Varecia variegata), in particular, has recently experienced rapid population declines due to habitat loss, ecological sensitivities to habitat degradation, and extensive human hunting pressure. Despite this, a recent study indicates that ruffed lemurs retain among the highest levels of genetic diversity for primates. Identifying how this diversity is apportioned and whether gene flow is maintained among remnant populations will help to diagnose and target conservation priorities. We sampled 209 individuals from 19 sites throughout the remaining V. variegata range. We used 10 polymorphic microsatellite loci and …

Behavioral And Neuroanatomical Abnormalities In Pleiotrophin Knockout Mice, Jason W. Krellman, Henry H. Ruiz, Veronica A. Marciano, Bracha Mondrow, Susan D. Croll

Publications and Research

Pleiotrophin (PTN) is an extracellular matrix-associated protein with neurotrophic and neuroprotective effects that is involved in a variety of neurodevelopmental processes. Data regarding the cognitive-behavioral and neuroanatomical phenotype of pleiotrophin knockout (KO) mice is limited. The purpose of this study was to more fully characterize this phenotype, with emphasis on the domains of learning and memory, cognitive-behavioral flexibility, exploratory behavior and anxiety, social behavior, and the neuronal and vascular microstructure of the lateral entorhinal cortex (EC). PTN KOs exhibited cognitive rigidity, heightened anxiety, behavioral reticence in novel contexts and novel social interactions suggestive of neophobia, and lamina-specific decreases in neuronal …

Case Study: From Gummy Bears To Celery Stalks: Diffusion And Osmosis, Kevin M. Bonney

Publications and Research

The article describes a case study which interperses information on diffusion and osmosis with content review and knowledge application questions, as well as simple experiment that can be conducted without the use of a laboratory. Topics discussed include biological membranes, the use of gummy bears to demonstrate osmosis and osmosis in animal cells. Also mentioned is osmosis in plants. It notes that the case study was developed for use in an introductory undergraduate biology course.

Naïve Hosts Of Avian Brood Parasites Accept Foreign Eggs, Whereas Older Hosts Fine-Tune Foreign Egg Discrimination During Laying, Csaba Moskát, Miklós Bán, Mark E. Hauber

Publications and Research

Background: Many potential hosts of social parasites recognize and reject foreign intruders, and reduce or altogether escape the negative impacts of parasitism. The ontogenetic basis of whether and how avian hosts recognize their own and the brood parasitic eggs remains unclear. By repeatedly parasitizing the same hosts with a consistent parasitic egg type, and contrasting the responses of naïve and older breeders, we studied ontogenetic plasticity in the rejection of foreign eggs by the great reed warbler (Acrocephalus arundinaceus), a host species of the common cuckoo (Cuculus canorus).

Results: In response to experimental parasitism before the …

Deep Two-Photon Microscopic Imaging Through Brain Tissue Using The Second Singlet State From Fluorescent Agent Chlorophyll Α In Spinach Leaf, Lingyan Shi, Adrián Rodríguez-Contreras, Yury Budansky, Yang Pu, Thien An Nguyen, Robert R. Alfano

Publications and Research

Abstract. Two-photon (2P) excitation of the second singlet (S2) state was studied to achieve deep optical microscopic imaging in brain tissue when both the excitation (800 nm) and emission (685 nm) wavelengths lie in the “tissue optical window” (650 to 950 nm). S2 state technique was used to investigate chlorophyll α (Chl α) fluorescence inside a spinach leaf under a thick layer of freshly sliced rat brain tissue in combination with 2P microscopic imaging. Strong emission at the peak wavelength of 685 nm under the 2P S2 state of Chl α enabled the imaging depth up to 450 μm through …

Gene Set Enrichment Analysis (Gsea) Of Toxoplasma Gondii Expression Datasets Links Cell Cycle Progression And The Bradyzoite Developmental Program, Matthew Mcknight Croken, Weigang Qiu, Michael W. White, Kami Kim

Publications and Research

Background Large amounts of microarray expression data have been generated for the Apicomplexan parasite Toxoplasma gondii in an effort to identify genes critical for virulence or developmental transitions. However, researchers’ ability to analyze this data is limited by the large number of unannotated genes, including many that appear to be conserved hypothetical proteins restricted to Apicomplexa. Further, differential expression of individual genes is not always informative and often relies on investigators to draw big-picture inferences without the benefit of context. We hypothesized that customization of gene set enrichment analysis (GSEA) to T. gondii would enable us to rigorously test whether …

Comparative Population Assessments Of Nautilus Sp. In The Philippines, Australia, Fiji, And American Samoa Using Baited Remote Underwater Video Systems, Gregory Jeff Barord, Frederick Dooley, Andrew Dunstan, Anthony Ilano, Karen N. Keister, Heike Neumeister, Thomas Preuss, Shane Schoepfer, Peter D. Ward

Publications and Research

The extant species of Nautilus and Allonautilus (Cephalopoda) inhabit fore-reef slope environments across a large geographic area of the tropical western Pacific and eastern Indian Oceans. While many aspects of their biology and behavior are now well-documented, uncertainties concerning their current populations and ecological role in the deeper, fore-reef slope environments remain. Given the historical to current day presence of nautilus fisheries at various locales across the Pacific and Indian Oceans, a comparative assessment of the current state of nautilus populations is critical to determine whether conservation measures are warranted. We used baited remote underwater video systems (BRUVS) to make …

Motor Cortex Electrical Stimulation Augments Sprouting Of The Corticospinal Tract And Promotes Recovery Of Motor Function, Jason B. Carmel, John H. Martin

Publications and Research

The corticospinal system—with its direct spinal pathway, the corticospinal tract (CST) – is the primary system for controlling voluntary movement. Our approach to CST repair after injury in mature animals was informed by our finding that activity drives establishment of connections with spinal cord circuits during postnatal development. After incomplete injury in maturity, spared CST circuits sprout, and partially restore lost function. Our approach harnesses activity to augment this injury-dependent CST sprouting and to promote function. Lesion of the medullary pyramid unilaterally eliminates all CST axons from one hemisphere and allows examination of CST sprouting from the unaffected hemisphere. We …

Acupuncture And Multiple Sclerosis: A Review Of The Evidence, H. I. Karpatkin, D. Napolione, B. Siminovich-Blok

Publications and Research

Use of acupuncture to treat multiple sclerosis (MS) is fairly common, but little literature exists which studies its effectiveness. The purpose of this paper is to review the literature on the use of acupuncture to treat MS. A literature search resulted in twelve peer-reviewed articles on the subject that examined the use of acupuncture to treat MS related quality of life (QoL), fatigue, spasticity, and pain. The majority of the studies were poorly designed—without control, randomization, or blinding. Description of the subjects, interventions, and outcome measures as well as statistical analysis was often lacking or minimal. Although many of the …

Early Back-To-Africa Migration Into The Horn Of Africa, Jason A. Hodgson, Connie J. Mulligan, Ali Al-Meeri, Ryan L. Raaum

Publications and Research

Genetic studies have identified substantial non-African admixture in the Horn of Africa (HOA). In the most recent genomic studies, this non-African ancestry has been attributed to admixture with Middle Eastern populations during the last few thousand years. However, mitochondrial and Y chromosome data are suggestive of earlier episodes of admixture. To investigate this further, we generated new genome-wide SNP data for a Yemeni population sample and merged these new data with published genome-wide genetic data from the HOA and a broad selection of surrounding populations. We used multidimensional scaling and ADMIXTURE methods in an exploratory data analysis to develop hypotheses …

Ecto-Protein Kinases And Phosphatases: An Emerging Field For Translational Medicine, Garif Yalak, Yigal H. Ehrlich, Bjorn R. Olsen

Publications and Research

Progress in translational research has led to effective new treatments of a large number of diseases. Despite this progress, diseases including cancer and cardiovascular disorders still are at the top in death statistics and disorders such as osteoporosis and osteoarthritis represent an increasing disease burden in the aging population. Novel strategies in research are needed more than ever to overcome such diseases. The growing field of extracellular protein phosphorylation provides excellent opportunities to make major discoveries of disease mechanisms that can lead to novel therapies. Reversible phosphorylation/dephosphorylation of sites in the extracellular domains of matrix, cell-surface and trans-membrane proteins is …

From Data Snore, To Data Score: Communicating Library Assessment Data Visually Through Infographics, Cassi Pretlow

CUNY Library Assessment Conference

This presentation was offered as part of the CUNY Library Assessment Conference, Reinventing Libraries: Reinventing Assessment, held at the City University of New York in June 2014.

Interactions Of Eukaryotic Translation Initiation Factors And 3' Untranslated Region Of Barley Yellow Dwarf Virus Mrna During Protein Synthesis: A Study Of Equilibrium Binding, Kinetics And Thermodynamics, Bidisha Banerjee

Dissertations, Theses, and Capstone Projects

Eukaryotic initiation factor (eIF) 4F binding to mRNA is the first committed step in cap-dependent protein synthesis. Barley Yellow Dwarf Virus (BYDV) employs a cap-independent mechanism of translation initiation which is mediated by a structural element BTE (BYDV translation element) located in the 3’ UTR of its mRNA. eIF4F bound the BTE and a translational inactive mutant with high affinity; thus questioning the role of eIF4F in translation of BYDV. To examine the effects of eIF4F in BYDV translation initiation, BTE mutants with widely different in vitro translation efficiencies ranging from 5-164% compared to WT were studied. Using fluorescence anisotropy …

Don't Push Me Over The (Knowl)Edge: The Social Correlates Of Latino High School Dropouts, Robert Charles Baskerville

Dissertations, Theses, and Capstone Projects

According to the forecast of the US Census Bureau, Latinos are the largest, fastest-growing ethnic group within the United States today and will comprise the majority of the US labor force sometime during the mid-21st century. Yet today, the youth of this diverse segment of the population are plagued by alarmingly high high school dropout rates, about double that of African-Americans youth and triple that of white youth. This yawning disparity prompts the examination of the social conditions contributing to this social crisis. How do demographic, aspirational, school-level, and socioeconomic variables affect the decision that so many Latino youth make …

Evolution And Ethics, Franklin Roy Bennett

Dissertations, Theses, and Capstone Projects

Does evolution inform the ancient debate about the roles that instinct (emotion/passion/sentiment/feeling) and reason do and/or should play in how we decide what to do? Evolutionary ethicists typically adopt Darwinism as a suitable explanation for evolution, and on that basis draw conclusions about moral epistemology. However, if Darwinism is to be offered as a premise from which conclusions about moral epistemology are drawn, in order to assess such arguments we must assess that premise. This reveals the highly speculative and metaphysical quality of our theoretical explanations for how evolution happens. Clarifying that helps to facilitate an assessment of the epistemological …

Between Sites: Critical Convergences At The Personal, Interpersonal, And Institutional Levels In A Service Learning Course, Kendra Rashaun Brewster

Dissertations, Theses, and Capstone Projects

Set within the context of the increasing emphasis on civic engagement and transformative education, this work addresses service learning as a form of civic engagement that holds both the risks of acriticality and critical potential. This study examines the capacity for the critical consciousness and relationality that define the primary commitments of critical service learning (see Kinefuchi, 2010). Thus, this study is grounded in the ways that the circuits of privilege and dispossession were breached in a service learning course where college students travelled to mentor adolescent girls who were in a secure residential facility. The narratives of former service …

Roles Of Dopamine D1 And D2, Opioid And Glutamate Nmda Receptor Signaling In The Acquisition And Expression Of Fat- And Glucose-Conditioned Flavor Preferences In Rats And C-Fos Analysis Of The Dopamine Mesotelencephalic And Nigrostriatal Pathways Following Intake Of Sugars And Fats In Rats, Julie Dela Cruz

Dissertations, Theses, and Capstone Projects

Systemic administration of the non-competitive NMDA antagonist, MK-801, demonstrated that acquisition, but not expression, were found to affect the orosensory-mediated (flavor/flavor: f/f) fructose-conditioned flavor preference (CFP). The present studies demonstrated a similar outcome when f/f and f/n processes were combined. Fat-CFP and glucose-CFP studies indicated that systemic injections of the NMDA antagonist, MK-801, were able to significantly reduce acquisition, but not expression, of Corn-Oil (CO)-CFP and glucose-CFP. Both studies appear to have mitigated effects on acquisition as compared to the separate orosensory and postingestive CFP studies.

Previous studies found that systemic administration of dopamine (DA) D1 (SCH23390) and D2 (raclopride) …

Mechanisms Of Regulation Of Mrna 3' Processing By P53 Pathway, Emral Cakmak Devany

Dissertations, Theses, and Capstone Projects

Although the p53 network has been intensively studied, genetic analyses long hinted at the existence of components that remained elusive. This dissertation focuses on the study of the regulation of mRNA 3' processing during DNA damage response (DDR) by the p53 pathway and the regulation of p53 expression by the mRNA 3' processing machinery. The results in this dissertation revealed new roles of tumor suppressor p53 in mRNA 3' processing. In Chapter II, I showed that p53 inhibits the cleavage step of polyadenylation reaction and that cells with different levels of p53 expression have different mRNA processing profiles. As part …

Environmental Progress Under China's New Leadership, Arielle Dumornay

Dissertations, Theses, and Capstone Projects

Environmental issues have been a consistent problem that many countries have had to adhere to. There have been many demands on global resources, it was not until the end of the 1960s did the ideals and visions about environmental protection begin to come to fruition. The UN in 1972 convened the United Nations Conference on the Human Environment in Stockholm which was considered to be a very pivotal moment in taking environmental problems more seriously. Declaring 19 principles that represent an environmental doctrine, this conference laid the groundwork for the new environmental agenda of the United Nations. The General Assembly …

Enchanted Entrepreneurs: The Labor Of Esoteric Practitioners In New York City, Karen Gregory

Dissertations, Theses, and Capstone Projects

Through participant observation and in-depth interviews, this dissertation weaves portraits of urban esoteric practitioners together with contemporary social theories of labor in order to explore the embodied and subjectifying project of becoming a psychic or intuitive practitioner capable of offering emotional and psychological "support" to city dwellers. By placing this project in a larger, contemporary political-economic framework, this dissertation looks to explore how spirituality is "entangled" (Bender 2010) in both social structures and cultural practices, as well as shifting configurations of work and the nature of labor. Here, we meet a network of individuals who are predominantly Tarot card readers …

Successful Aging: Use Of Communication Technology In An Adult Day Program, Wendy Johnson

Dissertations, Theses, and Capstone Projects

This dissertation investigated the relationship between learning digital communication technologies in an intergenerational intervention and successful aging among older adults. The specific goal of this study was to uncover the effects of this intervention on the cultural constructions of aging in an urban Adult Day Program in Trinidad and Tobago. This mixed method study utilized the Geriatric Depression Scale (GDS; Sheikh & Yesavage, 1986), a life satisfaction scale, well-being measurements, open-ended survey questionnaires and a focus group session. The results from the quantitative items indicated no significant differences after the intervention; however, the focus group discussion and open-ended surveys provided …

"We Are Refugees In Our Own Homeland": Land Dispossession And Resettlement Challenges In Post-Conflict Teso, Uganda, Matt Kandel

Dissertations, Theses, and Capstone Projects

This dissertation is based off of fieldwork that I conducted in post-conflict Teso region in northeastern Uganda from 2012-2013. It focuses primarily on land dispossession and challenges to resettlement. Conflicts over land intensified in the early 1990s, coinciding with the early stages of resettlement in southern Teso after a period of regional civil war and large-scale cattle rustling. In contrast to the large-scale "land grabs" in Sub-Saharan African that have occurred since the 2007-08 global commodities crisis, land expropriations occur mainly on a small-scale in Teso. I argue that there are a number of drivers to land dispossession in the …

An Equine-Facilitated Prison-Based Program: Human-Horse Relations And Effects On Inmate Emotions And Behaviors, Keren Bachi

Dissertations, Theses, and Capstone Projects

Policy makers and correctional authorities are seeking ways to enhance effectiveness of incarceration and reduce recidivism. Equine-facilitated prison-based vocational programs aim to rehabilitate inmates. Informed by the theories of attachment and desistance, this study evaluates the emotional and behavioral effects of such an intervention utilizing a quasi-experimental methodological triangulation design.

Recidivism and disciplinary misconduct are examined by clinical data-mining of institutional records. Propensity Score Matching, binary and multinomial logistic regressions are applied in a discrete-time event history analysis. Semi-structured interviews revealing the subjective experiences of participants are analyzed via the Listening Guide methodology. Quantitative questionnaires, exploring attachment and closeness to …

The Vastness Of Small Spaces: Self-Portraits Of The Artist As A Child Enclosed, Matthew John Burgess

Dissertations, Theses, and Capstone Projects

A tent of bed sheets, a furniture fort, a corner of the closet surrounded by chosen objects--the child finds or fashions these spaces and within them daydreaming begins. What do small spaces signify for the child, and why do scenes of enclosure emerge in autobiographical self-portraits of the artist? Sigmund Freud's theory that the literary vocation can be traced to childhood experiences is at the heart of this project, especially his observation that "the child at play behaves like a writer, in that he creates a world of his own, or rather, re-arranges the things of this world in a …

Structure And Function In Bacteriophage Phi6, James Carpino

Dissertations, Theses, and Capstone Projects

The present study of bacteriophage Phi6 has been preceded by a great number of exploratory studies of its structure and function, and these studies have formed a basis for Phi6's development into a model organism. In this study, two aspects of the model organism have been examined. 1. There are several uncharacterized and presumed untranslated regions (UTRs) in Phi6's 13.3 kilobase-pair dsRNA genome. I examined the impact of specific modification to the 3' UTR of the small segment of bacteriophage Phi6. I determined that modification to the purported UTR of the small segment resulted in severe fitness costs, supporting a …

Recruitment Of The Ribosomal 40s Subunit To The 3'Untranslated Region Of A Viral Mrna, Via The Eif4 Complex, Facilitates Cap-Independent Translation, Sohani Das Sharma

Dissertations, Theses, and Capstone Projects

Translation of uncapped plant viral RNAs can be facilitated by either an internal ribosomal entry site (IRES) in the 5' untranslated region (UTR) or a cap-independent translation element (CITE) in the 3' UTR. Barley yellow dwarf virus (BYDV) mRNA, which lacks both cap and poly(A) tail, has a translation element (3'BTE) in its 3' UTR that is essential for efficient translation initiation at the 5'-proximal AUG. This mechanism requires binding of the eukaryotic initiation factor 4G (eIF4G) subunit of the heterodimer eIF4F to the 3'BTE and base pairing between the 3'BTE and the 5' UTR. Here we investigate how this …

Comparative Phylogeography, Phylogenetics, And Population Genomics Of East African Montane Small Mammals, Terrence Constant Demos

Dissertations, Theses, and Capstone Projects

The Eastern Afromontane region of Africa is characterized by striking levels of endemism and species richness which rank it as a global biodiversity hotspot for diverse plants and animals including mammals, but has been poorly sampled and little studied to date. Using mtDNA and multi-locus nDNA sequence data, genome-wide RAD-Seq SNP data, and morphological data, I identify major cryptic biogeographic patterns within and between 11 co-distributed small mammal species/species groups across the Eastern Afromontane region. I focus on two endemic montane small mammal species complexes, Hylomyscus mice and Sylvisorex shrews, co-distributed across the Albertine Rift (AR) and Kenya Highlands (KH) …

Huu-Fa Thesis Dat?: A Syntactic Analysis Of Possessive Jamaican Creole Possessive Wh-Elements, Toni Ashlie Foster

Dissertations, Theses, and Capstone Projects

This thesis discusses the differences between the Jamaican Creole expressions huu-fa and fi-huu. Jamaican Creole is a language that was born from a combination of the lexifier language English and the substrate language Twi, therefore it is reasonable to check whether the features of JC were derived from these languages. The distribution of huu-fa and fi-huu resembles the distribution of English whose. Fi-huu and huu-fa are WH-elements that show possession, similar to the English word whose. They are made of a WH-pronoun and a form of the preposition fi "for". Both terms differ in internal structure, and distribution. The difference …

Homeostasis-Driven Responses To Consumer Sensations, Rhonda Hadi

Dissertations, Theses, and Capstone Projects

This research examines the effect of experienced physical temperature on an individual's decision-making process. Reliance on emotions can function as a warming process and reliance on cognitions can function as a cooling process, hence individuals are nonconsciously induced to alter their decision-making style according to their thermoregulatory objectives. My first two studies support a thermoregulatory account by demonstrating that the mere use of cognitive versus affective processing leads to both self-reported and objective changes in body temperature, and that the adoption of a compensatory pathway can indeed aid in providing temperature-related comfort. My last three studies demonstrate that individuals adopt …

Reading Nation In Translation: The Spectral Transnationality Of The Malaysian Racial Imaginary, Fiona Hsiao Yen Lee

Dissertations, Theses, and Capstone Projects

In recent decades, literary studies has experienced a global turn, often understood as a move beyond national paradigms of analysis, which are deemed to be narrow and particularistic. Although wary of the tacit universalizing tendencies of global frames, scholars of race and postcoloniality have critically embraced the global by arguing for the need to theorize transnationalism from marginalized perspectives. However, casting the global and the national in oppositional terms ignores the fact that national racial ideologies both actively shape and are shaped by globally circulating ideas about race. An understudied site in postcolonial studies, Malaysia--formerly known as Malaya--is an exemplary …