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The Proteomic Response To Acute Heat Stress After Acclimation To Fluctuating Temperatures In The Eurythermal Porcelain Crab, Petrolisthes Cinctipes, Michael A. Garland, Holland Elder, David A. Hurt, Jonathon H. Stillman, Lars Tomanek

Biological Sciences

The porcelain crab Petrolisthes cinctipes inhabits the high intertidal along the Pacific coast between British Columbia and central California and is an emerging model organism to study the effects of environmental stress. In its habitat it experiences not only variability in average temperature but also variability in temperature fluctuations. To investigate the effect of temperature fluctuations, we characterized the heat shock response at 30°C for 6 h by acclimating individuals to three different acclimation treatments: 10°C, 10-20°C (4 h), and 10-30°C (4 h) on a daily basis for one month. We dissected claw muscle and separated proteins by 2D gel …

Experimentally Altered Navigational Demands Induce Changes In The Cortical Forebrain Of Free-Ranging Northern Pacific Rattlesnakes (Crotalus O. Oreganus), Matthew L. Holding, Julius A. Frazier, Emily N. Taylor, Christine R. Strand

Biological Sciences

The hippocampus of birds and mammals plays a crucial role in spatial memory and navigation. The hippocampus exhibits plasticity in adulthood in response to diverse environmental factors associated with spatial demands placed on an animal. The medial and dorsal cortices of the telencephalon of squamate reptiles have been implicated as functional homologues to the hippocampus. This study sought to experimentally manipulate the navigational demands placed on free-ranging northern Pacific rattlesnakes (Crotalus o. oreganus) to provide direct evidence of the relationship between spatial demands and neuroplasticity in the cortical telencephalon of the squamate brain. Adult male rattlesnakes were radio-tracked for 2 …

The Proteomic Response Of Mytilus Californianus To Acute Oxidative Stress In The Presence Of A Sirtuin Inhibitor, H. Chilton, J. Mier, M. Zuzow, L. Tomanek

Biological Sciences

The ribbed mussel (Mytilus californianus) occurs in the in the rocky intertidal zone along the Pacific coast of North America where it experiences greatly varying environmental conditions in terms of temperature and hypoxia, stressors that are known to induce oxidative stress. It has been hypothesized that while under acute heat stress, related Mytilus congeners undergo a shift in redox potential through the reduction of NADH fueled respiration pathways to the production of the reducing agent NADPH as a potential defensive mechanism against the production of reactive oxygen species. In addition, it has been hypothesized that sirtuins (a family …

Proteomic Responses Of Mytilus Congeners To Hypo-Saline Stress, M. Z. Zuzow, L. Serafini, L. Hitt, J. Valenzuela, L. Tomanek

Biological Sciences

The invading Mediterranean blue mussel species Mytilus galloprovincialis requires higher salinity levels than the more hypo-saline tolerant and native M.trossulus,which it replaced from its southern range in California, possibly due to climate change.It is also the warm-adapted congener.Hypo-salinity,due to an increase in extreme precipitation events,has been hypothesized to limit the expansion of the invader.By comparing the proteomic responses of the congeners to acute hypo-saline stress we were trying to identify the interspecific differences that set limits to hypo-saline stress and assess their contribution to the range shifts.We exposed mussels to acute hypo-saline(100%, 85% and 70% salinity)stress for 4h followed …

Proteomic Analysis Of Hyposalinity Stress In The Ascidian Species Ciona Savignyi And C. Intestinalis, James S. Koman, Lars Tomanek

Biological Sciences

The ascidian species Ciona savignyi and C. intestinalis are invasive species but show interspecific differences in their population response to hypo-saline stress associated with heavy winter-run off events that are predicted to become more frequent due to climate change. Despite an almost world-wide distribution, C. intestinalis seems to be more susceptible to hypo-saline stress than the geographically more limited C. savignyi. Given that the genomes of both species are fully sequenced, we were able to compare their proteomic response to both acute and chronic salinity to characterize the mechanisms that are responsible for setting tolerance limits to hyposaline conditions …

Proteomic Response Of Tidal-Rhythm Entrained Mytilus Californianus To Acute Aerial Heat Stress, D. Hernandez, M. Schuman, L. Tomanek

Biological Sciences

The rocky intertidal mussel species Mytilus californianus is native to the Pacific coast of North America. It is frequently exposed to temperatures that can induce the cellular stress response due to the tidal rhythm. Instead of acclimating mussels to common conditions in the laboratory for several weeks, we collected mussels from a site with mussel body temperature recordings (specimens’ thermal history) and immediately started experimentation by mimicking the tidal rhythm with different levels of heat stress over multiple low tides and with different recovery periods. By using individuals that were entrained to the tidal rhythm and by maintaining it, we …

Statistics Department 2012 Newsletter, Statistics Department

Statistics Department Newsletter

No abstract provided.

A Comparative Study Of Botryllid Tunicates Regeneration In The San Francisco Bay, Michelle Wray, C. Sarah Cohen

STAR Program Research Presentations

Regeneration of body parts is a remarkable phenomenon that is shared by phylogenetically diverse organisms, including some chordates. Lizards can regenerate their tails and sea stars their arms when injured by predators. Humans can even regenerate small portions of the liver. However, colonial botryllid tunicates, basal chordates, have the incredible ability to regenerate the entire functioning body (zooids) from only a small sample of vasculature in a process called vascular budding. Regeneration processes for various botryllid species show some characteristic features in laboratory studies using application of retinoic acid as a developmental initiator. The three botryllid species, Botrylloides violaceus (n …

Informing Drug Design: Human Acetylcholinesterase Response To Organophosphate Poisoning, Aiyana Emigh, Sebnem Essiz-Gokhan, Brian Bennion

STAR Program Research Presentations

Acetylcholinesterase (AChE) is target enzyme of organophosphate (OP). Curent treatments for OP poisoning i.e. oximes, have limited success, especially without pre-treatment. This study uses molecular dynamic analysis to shine light on structure and dynamical fluctuations of free AChE and OP-inhibited AChE. Knowledge gained by the study of OP inhibition of acetylocholinesterase should guide future drug designs of more effective antitoxins.

First Record Of Serropalpus Substriatus Haldeman, 1848 (Coleoptera: Melandryidae) On Giant Sequoia, Sequoiadendron Giganteum (Lindl.) J. Buchholz (Cupressaceae): New Larval Host, Kim S. Camilli, Joshua D. Soderlund, David Wood, Jim Kral, Douglas D. Piirto

Natural Resources Management and Environmental Sciences

No abstract provided.

Where We Stand: 1975-2011, Laura Sorvetti, Catherine Trujillo, Josh Harmon

Creative Works

Where We Stand is an extension of the 2009 exhibit Strive & Struggle: Documenting the Civil Rights Movement at Cal Poly, 1967 – 1975 and similarly draws from Mustang Daily articles, student club documents, and oral accounts to construct a brief history of African American advocacy on the Cal Poly campus from the Civil Rights Movement to the present day.

The exhibit documents the roles that student and faculty organizations played in building awareness of issues of diversity and identity on campus. Assessing their efforts reveals both what has changed since 1975 and what remains to be addressed on the …

The Discovery Of Insulin: A Case Study Of Scientific Methodology, William D. Stansfield

Biological Sciences

The nature of scientific research sometimes involves a trial-and-error procedure. Popular reviews of successful results from this approach often sanitize the story by omitting unsuccessful trials, thus painting the rosy impression that research simply follows a direct route from hypothesis to experiment to scientific discovery. The discovery of insulin is a classical case study in this genre that begs for an explanation to our students because it is so often ignored or misrepresented even in biology and physiology textbooks.

Bioluminescence In The High Arctic During The Polar Night, J. Berge, A.S. Båtnes, G. Johnsen, S.M. Blackwell, Mark A. Moline

Biological Sciences

This study examines the composition and activity of the planktonic community during the polar night in the high Arctic Kongsfjord, Svalbard. Our results are the first published evidence of bioluminescence among zooplankton during the Arctic polar night. The observations were collected by a bathyphotometer detecting bioluminescence, integrated into an autonomous underwater vehicle, to determine the concentration and intensity of bioluminescent flashes as a function of time of day and depth. To further understand community dynamics and composition, plankton nets were used to collect organisms passing through the bathyphotometer along with traditional vertical net tows. Additionally, using a moored bathyphotometer closed …

Design And Experimental Validation Of Continuous Dna Separation Using Itp-Based Microfluidic Device, Nora C. M. Goscinski, Mary E. Rosa, Elizabeth K. Wheeler, Maxim Shusteff

Chemistry and Biochemistry

We have demonstrated a chip-based microfluidic device suitable for rapid and high-throughput DNA separation and purification for downstream analysis. The glass-based device incorporates a transverse free-flow isotachophoresis (tFF-ITP) that overcomes the volume limitations of CE approaches, and allows continuous sample processing in contrast to batch-mode solid-phase extraction (SPE). The device is able to focus DNA at flow rates up to 100 uL/min, and sample conductivity up to 2 mS/cm. Downstream of the chip, 30-40% of DNA from the input sample is recovered as a result of ITP focusing, and preliminary results indicate that this design is able to purify DNA …

Β-Casein–Phospholipid Monolayers As Model Systems To Understand Lipid–Protein Interactions In The Milk Fat Globule Membrane, Sophie Gallier, Derek E. Gragson, Rafael Jiménez-Flores, David W. Everett

Chemistry and Biochemistry

Phospholipid–protein monolayer films were studied as model systems to mimic the structure of the native bovine milk fat globule membrane (MFGM) and to understand lipid–protein interactions at the surface of the globule. Phospholipids extracted from bovine raw milk, raw cream, processed milk and buttermilk powder were spread onto the air–water interface of a Langmuir trough, β-casein was then added to the sub-phase, and Langmuir–Blodgett films were studied by epifluorescence microscopy and atomic force microscopy. In all films, β-casein was responsible for clustering of the sphingomyelin- and cholesterol-rich microdomains into larger platforms. This suggests that the same phenomenon may happen at …

New Metrics For Managing And Sustaining The Ocean’S Bounty, Heather Tallis, Sarah E. Lester, Mary Ruckelshaus, Mark Plummer, Karen Mcleod, Anne Guerry, Sandy Andelman, Margaret R. Caldwell, Marc Conte, Stephen Copps, David Fox, Rod Fujita, Steven D. Gaines, Guy Gelfenbaum, Barry Gold, Peter Kareiva, Choong-Ki Kim, Kai Lee, Michael Papenfus, Scott Redman, Brian Silliman, Lisa Wainger, Crow White

Biological Sciences

Policies are arising around the world, most recently in the United States, that mandate the implementation of marine spatial planning as a practical pathway towards ecosystem-based management. In the new United States ocean policy, and several other cases around the globe, ecosystem services are at the core of marine spatial planning, but there is little guidance on how ecosystem services should be measured, making it hard to implement this new approach. A new framework is shown here for practical, rigorous ecosystem service measurement that highlights contributions from both natural and social systems. The novel three-step framework addresses traditional shortcomings of …

Mycorrhizal Colonization Of Hypericum Perforatum L. (Hypericaceae) From Serpentine And Granite Outcrops On The Deer Isles, Maine, Naveed Davoodian, Jason Bosworth, Nishanta Rajakaruna

Biological Sciences

Given the paucity of literature on plant-fungal interactions on serpentine soils and limited investigation of serpentine geoecology in eastern North America, we examined mycorrhizal colonization of Hypericum perforatum from adjacent serpentine and granite outcrops on the Deer Isles, ME to determine whether plants were differentially colonized based on substrate. We coincided our sampling with three phenologic stages of H. perforatum (preflowering, flowering, postflowering) to determine possible differences in colonization based on plant phenology. The levels of mycorrhizal colonization in H. perforatum were not significantly different between serpentine and granite sites, while levels of colonization in postflowering plants were significantly higher …

A Collaborative Approach To Investigate Site Fidelity, Home Range, And Homing Behavior Of Cabezon (Scorpaenichthys Marmoratus), Carlos Mireles, Royden Nakamura, Dean Wendt

Biological Sciences

Understanding the area use requirements of species targeted for protection by marine protected areas (MPAs) is critical to the future conservation efforts of economically important fish species. Knowledge of home range size and site fidelity is essential in determining whether species will benefit from the protection offered by a MPA, the size of the area needed to protect individuals, and the extent to which surrounding unprotected areas may be supplemented through post-recruitment movement or “spillover”. We utilized a traditional mark and recapture approach, along with GIS spatial analysis to investigate the site fidelity, home range, and homing behavior of the …

Agriview, Spring 2012, College Of Agriculture, Food, And Environmental Sciences: California Polytechnic State University - San Luis Obispo

AGRIVIEW

No abstract provided.

Forum History At Forty (2012), David Hafemeister

Physics

Physics is a major component of many of society's difficult issues: nuclear arms and their proliferation, energy shortages and energy impacts, climate change, and technical innovation. Because physics principles underlie so many of these societal issues and because physics offers a way to quantify some aspects of them, members of the American Physical Society (APS) should be encouraged to understand, analyze and debate them. This is precisely why APS members formed the Forum on Physics and Society (FPS). To those of us who have been involved in FPS affairs for a long time, it seems like only yesterday that we …

Improving Model Performance Using Season-Based Evaluation, Misgana Muleta

Civil and Environmental Engineering

Computer models have become vital decision-making tools in many areas of science and engineering including water resources. However, models should be properly evaluated before use to improve the likelihood of making sound decisions based on their results. The model evaluation technique practiced today in hydrology assumes that model parameters are season insensitive and attempts to identify “optimal” values that would describe watershed behavior during dry and wet seasons. This assumption could compromise accuracy of model predictions. This study demonstrates performance improvement that would be achieved when a season-based model evaluation approach is pursued. A global sensitivity analysis (SA) model has …

Exploring The Physics With Computer Animation, T. J. Bensky

Physics

No abstract provided.

Automatic Document Classification In Small Environments, Jonathan David Mcelroy

Master's Theses

Document classification is used to sort and label documents. This gives users quicker access to relevant data. Users that work with large inflow of documents spend time filing and categorizing them to allow for easier procurement. The Automatic Classification and Document Filing (ACDF) system proposed here is designed to allow users working with files or documents to rely on the system to classify and store them with little manual attention. By using a system built on Hidden Markov Models, the documents in a smaller desktop environment are categorized with better results than the traditional Naive Bayes implementation of classification.

Fluid Elasticity Can Enable Propulsion At Low Reynolds Number, Nathan C. Keim, Mike Garcia, Paulo E. Arratia

Physics

Conventionally, a microscopic particle that performs a reciprocal stroke cannot move through its environment. This is because at small scales, the response of simple Newtonian fluids is purely viscous and flows are time-reversible. We show that by contrast, fluid elasticity enables propulsion by reciprocal forcing that is otherwise impossible. We present experiments on rigid objects actuated reciprocally in viscous fluids, demonstrating for the first time a purely elastic propulsion set by the object’s shape and boundary conditions. We describe two different artificial “swimmers” that experimentally realize this principle.

Determining Cell Markers For The Evaluation Of Huvec And Huvsmc Phenotypes On Formalin Fixed, Paraffin Embedded Blood Vessel Mimics, Christopher Michael Miracle

Biomedical Engineering

No abstract provided.

Cal Poly Professor, Colleagues Discover New Magnetic Bacterium, Public Affairs Office

Press Releases

No abstract provided.

Why Animal Ethics Committees Don't Work, Denise Russell

Between the Species

Abstract

Animal ethics committees have been set up in many countries as a way to scrutinize animal experimentation and to assure the public that if animals are used in research then it is for a worthwhile cause and suffering is kept to a minimum. The ideals of Refinement, Reduction and Replacement are commonly upheld. However while refinement and reduction receive much attention in animal ethics committees the replacement of animals is much more difficult to incorporate into the committees’ deliberations. At least in Australia there are certain structural reasons for this but it is likely that most of the reasons …

Ap French Language And Culture: Strategizing For Success, Brian G. Kennelly

World Languages and Cultures

No abstract provided.

Polymorphic Variation Of The Dgat1 Gene In Cal Poly Holstein And Jersey Breeds And The Correlation Of Milk Fat Content, And Genetic Values, Andrea M. Laubscher

Master's Theses

The selection of dairy cattle is dictated by improvement of yield and composition of milk. Genotyping tests give breeders the opportunity to use previously unavailable information to increase the frequency of beneficial alleles in their herds. A QTL influencing milk yield and composition is located in the centromeric end of chromosome 14. The polymorphism being analyzed is a K232A substitution (lysine to alanine amino acid change) in exon VII of the diacylglycerol acyl transferase 1 (DGAT1) gene. DGAT1 is a key acyltranferase in triglyceride biosynthesis. DGAT1 encodes an enzyme which catalyzes the final step of mammalian triglyceride synthesis from carbohydrates. …

Animals-As-Patients: Improving The Practice Of Animal Experimentation, Jane Johnson, Christopher Degeling

Between the Species

In this paper we propose a new way of conceptualizing animals in experimentation – the animal-as-patient. Construing and treating animals as patients offers a way of successfully addressing some of the entrenched epistemological and ethical problems within a practice of animal experimentation directed to human clinical benefit. This approach is grounded in an epistemological insight and builds on work with so-called ‘pet models’. It relies upon the occurrence and characterization of analogous human and nonhuman animal diseases, where, if certain criteria of homology and mechanism are met, the animal simultaneously becomes a patient and a spontaneous model of the human …