Digital Commons Network^™

Detection Of Viable Microorganisms Using Propidium Monoazide, Erik J. Mcfarland, Adrian Ponce Dr.

STAR Program Research Presentations

Propidium monoazide (PMA) is a molecular tool used to assess viability of microorganisms. Currently, PMA is thought to discern viability through membrane permeability; PMA enters only membrane compromised cells, irreversibly crosslinks to theirDNAand precipitates theDNAout of solution, preventing it from being amplified during polymerase chain reaction (PCR). Using PMA on a sample of live and dead microorganisms results in only theDNAof living organisms being amplified and identified. Therefore, a comparison ofPCRresults with and without PMA allows one to determine the live fraction and total population, respectively.

Current literature provides conflicting evidence as to the effectiveness of the technique. Our research …

Disocvering Ionic Liquid Resistant Genes, Bree Person, Douglass Higgins, Michael Thelen

STAR Program Research Presentations

: Plant biomass is a rich source of sugars that can be converted to biofuels by engineered microbes. However, because the lignocellulose in biomass is insoluble in aqueous conditions and recalcitrant to enzymatic degradation, thermochemical treatment is required to break apart the lignin and cellulose polymers before sugars can be released. The most effective chemicals for doing this are known as ionic liquids, which are salts that are molten at temperatures below 100° C. Although these solvents have many unique properties that are ideal for solubilizing lignocellulose, they have been found to inhibit the growth of bacterial strains used to …

Seeing Through A Cloudy Glass: Putting Limits On Planetary Nebulae Abundances Using Photoionization Modeling., Peter R. Sullivan, Ravi Sankrit

STAR Program Research Presentations

Planetary nebulae (PNe) form around low to intermediate mass stars transitioning from the giant branch to white dwarf phase. The outer layer of the star is ejected during the transition and this gas, ionized by the central star, emits a line-spectrum. This spectrum traces the chemical abundances that were characteristic of the interstellar medium in which the star formed (e.g. oxygen) as well as of the elements created by these progenitor stars (e.g. nitrogen) aiding our understanding of chemical evolution of galaxies. In this project, we use modeling of the emission lines of PNe to determine the accuracy of direct …

Using Stable Isotope Analysis Of Zooplankton To Document Trophic And Biogeochemical Changes In The San Francisco Estuary, Steven C. Westbrook, Julien Moderan

STAR Program Research Presentations

Zooplankton represent a vital link between phytoplankton and fish, like the endangered Delta Smelt. Human interferences (nitrates from waste water, flow alteration, invasive species introduction…) have altered the structure of the San Francisco Estuary (SFE) ecosystem. We use stable isotope analysis to improve our knowledge of the planktonic food web in the SFE and gain insights into its evolution over the past decades. We use the ratios of certain isotopes (Nitrogen, Carbon, Sulfur, etc.) in different species of zooplankton to tell us what it is feeding on as well as the trophic level it feeds in. My research focused on …

Chemical Compatibility Study Of Anti-Corrosive Materials For Stratospheric Observatory For Infrared Astronomy (Sofia), Belyn Nicole Grant, Zaheer Ali, Greg Perryman, Stefan Teufel, Brian Eney

STAR Program Research Presentations

The Stratospheric Observatory for Infrared Astronomy (SOFIA) is a telescope designed to capture infrared light from deep space, mounted in a highly modified Boeing 747 SP. Portions of the aircraft interior are showing signs of corrosion, and need to be coated with a material that will prevent further corrosion. Up to date, current anti-corrosive materials commonly used on aircrafts are incompatible with the very thin aluminum surface of the telescope mirrors. The purpose of our study is to find an effective corrosion preventive material with low outgassing properties.

Beneath The Surface Overview, Elizabeth Sikkema

Biological Sciences

As a Wildlife Biology major at Cal Poly, I was able to express my passion for the ocean and beaches in planning and co-designing a beach and dive clean up Earth Day celebration as my senior project. This event took place at Avila Beach on April 21st and was hosted by the Central Coast Aquarium. It was open to the public in attempts to promote outreach and awareness of coastal conservation, allowing volunteers to participate in the clean-ups and visit the Aquarium. The event was a success, involving over 100 volunteers, collecting and recycling 150 pounds of trash and also …

Archaeological Perspectives On Prehistoric Conservation In Western North America, Terry L. Jones

Social Sciences

The archaeological record represents a potentially critical source of information on past relationships between human hunters and populations of game animals. Archaeological research in the last 40–50 years has produced two alternative views on these relationships: one that Native people were knowledgeable, benevolent conservators of game and an alternative that suggests that they depleted and suppressed game populations through overhunting. A brief review of the history of this research shows that neither position is well-supported by empirical facts and that researchers have demonstrated a certain overzealousness in attempting to support one or the other of these interpretations. The facts are …

Investigation Of A Pregnancy Lifestyle Intervention Using Mediation Analysis And A Power Analysis Simulation, Kelsey Grantham

Statistics

No abstract provided.

Rainwater To Flush Toilets On A Volcano, Robert A. Buchan

STAR Program Research Presentations

Objective: Investigate the feasibility of a rainwater collection system to provide for a sustainable source of non-potable or potable water at a potentially isolated solar observatory site (likely Mauna Loa Observatory in Hawaii).

Introduction: The National Center for Atmospheric Research/High Altitude Observatory (NCAR/HAO) proposes to build a solar observatory comprising a trio of instruments for studying solar coronal magnetism.

Methods: Each square foot of collection surface will experience approximately 0.62 gallons of runoff from 1 inch of rainfall (12 in x 12 in x 1 in = 0.62 gallons). Some water will be lost to evaporation, splashing, overflows, and other …

Statistics Department 2013 Newsletter, Statistics Department

Statistics Department Newsletter

No abstract provided.

Table Of Contents

Cal Poly Magazine

No abstract provided.

Optimizing Electrode Design For Microbial Fuel Cells Used For Wastewater Treatment, Lindsay Nichols, John A. Hogan

STAR Program Research Presentations

Microbial fuel cells (MFC) utilize bacteria to generate an electrical current that can be used in the decomposition of sludge and human urine. In a MFC there is an anode (for oxidation of organic compounds), cathode (reduction of oxygen or carbon dioxide), and a proton exchange membrane (PEM, allows protons to migrate); reduction-oxidation reactions between the anode and cathode produce a measurable current. Bacteria that are found in sludge can be used to produce electrons in a voltaic cell, but optimizing conditions for harnessing the energy is crucial to making a MFC efficient. Research has shown that the ratios of …

Cal Poly Magazine, Winter 2012

Cal Poly Magazine

No abstract provided.

A Tunable Electromagnetic Band-Gap Microstrip Filter, Greg A. Lancaster

Master's Theses

In high frequency design, harmonic suppression is a persistent struggle. Non-linear devices such as switches and amplifiers produce unwanted harmonics which may interfere with other frequency bands. Filtering is a widely accepted solution, however there are various shortcomings involved. Suppressing multiple harmonics, if desired, with traditional lumped element and distributed component band-stop filters requires using multiple filters. These topologies are not easily made tunable either. A new filter topology is investigated called Electromagnetic Band-Gap (EBG) structures.

EBG structures have recently gained the interest of microwave designers due to their periodic nature which prohibits the propagation of certain frequency bands. EBG …

Breaking Weak 1024-Bit Rsa Keys Using Cuda, Kerry Scharfglass, Darrin Weng, Joseph White, Christopher Lupo

Computer Science and Software Engineering

An exploit involving the greatest common divisor (GCD) of RSA moduli was recently discovered [1]. This paper presents a tool that can efficiently and completely compare a large number of 1024-bit RSA public keys, and identify any keys that are susceptible to this weakness. NVIDIA's graphics processing units (GPU) and the CUDA massively-parallel programming model are powerful tools that can be used to accelerate this tool. Our method using CUDA has a measured performance speedup of 27.5 compared to a sequential CPU implementation, making it a more practical method to compare large sets of keys. A computation for finding GCDs …

December 5, 2012 Cal Poly Report, Public Affairs Office

Cal Poly Report

No abstract provided.

Solar Energy Collection Analysis Tool For Conceptual Aircraft Design, Grant Glazebrook

Aerospace Engineering

As battery energy storage and solar cell technology improve, solar aircraft are increasingly being considered for High Altitude Long Endurance missions. Although solar vehicles may theoretically remain on-station indefinitely using the sun as a power source, their design and feasibility is sensitive to mission planning details as specific as the time history of the vehicle’s deck orientation relative to the sun; the energy available for capture by the on-board solar array is governed by the solar incidence angle, and at certain orientations, the vehicle may cast shadows on itself and further reduce its energy capture capabilities. To quantify these losses, …

An Analysis Of Risk Reduction Choices In Dcis Breast Cancer Patients, Lauren Soltesz

Statistics

The main focus of this paper was to evaluate possible demographic and clinical characteristics associated with a woman’s choice of breast conserving surgery (BCS), unilateral mastectomy (ULM), or bilateral risk reduction mastectomy (BRRM). The cohort consisted of patients presenting to the City of Hope National Medical Center with ductal carcinoma in situ breast cancer who elected to have cancer directed surgery (N=305). Analyses to examine associations of patient characteristics with type of surgery were conducted using a multinomial logistic regression. Results showed that older women were more likely to choose breast conserving surgery over bilateral risk reduction mastectomy than younger …

Landscape Design, Cost Estimate, And Management Plan, Garrett Talbot Williams

Horticulture and Crop Science

The purpose of this project is to evolve a bleak landscape into something that the home owner can appreciate and love. This project will demonstrate the knowledge of this process to provide as a guide for others. This project gives an overview of all aspects of a landscape project including: drawings of an irrigation layout, planting plan, drainage plan, and a construction plan. This project takes place in Riverbank, California, which is located in the Central Valley. The property’s main centerpiece is a pool and adjacent waterfall. However, the landscape currently surrounding the pool is very bare and lacks aesthetic …

Investigation Of Optical Dipole Traps For Trapping Neutral Atoms For Quantum Computing, Danielle May

Physics

No abstract provided.

Analysis Of Alcohol Use Among Pregnant Women In San Luis Obispo County, Samantha Law

Statistics

Drinking alcohol during pregnancy is harmful to the fetus, and can lead to serious alcohol related developmental birth defects. Utilizing prenatal screening, such as the 4P’s Plus© screening tool, during a woman’s first prenatal doctors visit can help educate women and reduce continued alcohol use during pregnancy. Currently the CDC reports that 1 in 13 women in the US drink alcohol while pregnant compared to local reports that 1 in 3 women in San Luis Obispo County continue to drink alcohol during pregnancy. A primary concern for many local county health care experts and organizations is to raise awareness that …

Space Vehicle Testing, Charlotte Ann Belsick

Master's Theses

Requirement verification and validation is a critical component of building and delivering space vehicles with testing as the preferred method. This Master’s Project presents the space vehicle test process from planning through test design and execution. It starts with an overview of the requirements, validation, and verification. The four different verification methods are explained including examples as to what can go wrong if the verification is done incorrectly. Since the focus of this project is on test, test verification is emphasized. The philosophy behind testing, including the “why” and the methods, is presented. The different levels of testing, the test …

Early Forest Fire Detection Using Texture, Blob Threshold, And Motion Analysis Of Principal Components, Georges Fouad Moussa

Master's Theses

Forest fires constantly threaten ecological systems, infrastructure and human lives. The purpose behind this study is minimizing the devastating damage caused by forest fires. Since it is impossible to completely avoid their occurrences, it is essential to accomplish a fast and appropriate intervention to minimize their destructive consequences. The most traditional method for detecting forest fires is human based surveillance through lookout towers. However, this study presents a more modern technique. It utilizes land-based real-time multispectral video processing to identify and determine the possibility of fire occurring within the camera’s field of view. The temporal, spectral, and spatial signatures of …

Smart Wall Plug Design For The Dc House Project, Edward Constant Sibal

Master's Theses

The DC House project at Cal Poly State University faces a challenge of supplying DC voltage to household appliances. Each appliance in the DC House constitutes a DC load that has a unique voltage and power rating, hence the need to develop a smart DC wall plug that will automatically adjust to the operating voltage required by any DC load. This thesis entails a proof of concept design of the smart DC wall plug which can automatically detect an appliance’s voltage rating. The design employs a dc-dc converter in conjunction with a microcontroller to sense load current to properly adjust …

The Application Of Systems Engineering Principles To Model Lithium Ion Battery Voltage, George Gibbs

Master's Theses

The objective of this project is to present a Lithium Ion battery voltage model derived using systems engineering principles. This paper will describe the details of the model and the implementation of the model in practical use in a power system. Additionally, the model code is described and results of the model output are compared to battery cell test data. Finally, recommendations for increased model fidelity and capability are summarized.

The modeling theory has been previously documented in the literature but detailed implementation and application of the modeling theory is shown. The detailed battery cell test voltage profiles are proprietary; …

Aspects Of General Relativity In 1+1 Dimensions, Richard D. Mellinger Jr

Physics

What would be the properties of a universe with only one spatial dimension and one time dimension? General relativity in 1+1 dimensions is unique since the two curvature terms in the Einstein field equations cancel. This makes the Einstein field equations algebraic rather than differential equations. This special feature can make 1+1 dimensionality attractive as an instructional tool to simplify the mathematics that many beginners find opaque. We explore the implications and features of the Einstein field equations in 1+1 dimensions and find they provide a surprisingly rich and interesting model. We then study an alternate theory and its implications …

Communication Of Recycling Through Labeling And Packaging, Serena Mistry

Graphic Communication

Recycling has become a more prevalent practice in the last few decades. However, the labeling system on today’s packaging is not always clear in indicating whether something is recyclable. Recently, there has been a great deal of attention directed at a new system, known as the How2Recycle label, an innovative labeling system expected to launch in the next few years. As the population continues to grow at unprecedented levels and more strain is placed on the global environment, recycling becomes more crucial. In the United States, the lack of a standard system for recycling on labeling and packaging deters people …

Determining Stellar Velocity Dispersion In Active Galaxies: Is The [Oiii] Width A Valid Surrogate?, Kelsi Flatland

Physics

The tight empirical relation between the stellar velocity dispersion (sigma) of the bulge and the mass of the supermassive black hole (BH) at its center indicates a close connection between galactic evolution and BH growth. The evolution of this relation with cosmic time provides valuable clues to its origin. While the mass of the BH can be easily estimated using the Doppler broadening of the Hbeta emission line in type I active galactic nuclei (AGNs), measuring sigma simultaneously is challenging, since the nuclear emission outshines the host galaxy. Thus, it is highly desirable to find an alternative way to estimate …

Automated Student Code Assessment With Symbolic Execution And Java Pathfinder, Karl Bell

Master's Theses

The assessment of student code is a necessary part of most programming courses. However, many ways of assessing the correctness of student code can be very time-consuming and may be error-prone. This paper presents JSymTester, a tool which uses the symbolic execution framework of the Java PathFinder to find test inputs for student code and uses these inputs to extensively compare its functionality to a reference implementation. This allows for automatic testing of student code, relying only on the reference implementation and the student's own implementation, eliminating the need to manually write tests. This tool was tested on small assignments …

Implementation And Validation Of Fault-Rupture Response Spectrum Analysis Procedure In Csibridge For Bridges Crossing Earthquake Fault Ruptures, Jennifer Evelyn Tures

Master's Theses

This thesis evaluates the application of a simplified analysis procedure as implemented in version 16 of CSiBridge^TM for design of bridges crossing earthquake fault ruptures. The fault-rupture response spectrum analysis (FR-RSA) approximation method has been proved adequate for both straight and curved ordinary bridges, but lacked a comfortable interface to accommodate the method users. Computers and Structure, Inc. has implemented the FR-RSA procedure into CSiBridge^TM, a user-friendly integrated 3-D bridge design software, as an added seismic design feature. By combining the response of the bridge due to the quasi-static displacement from the fault strike-slip rupture and the …