Digital Commons Network^™

Identifying Molecular Markers Associated With Salt And Boron Tolerance In Poplar Trees, Darshanpreet Gill, Nathan Follen, Davis W. Cheng, Kyan Salehi, Gary Banuelos, James P. Prince

STAR Program Research Presentations

Standard irrigation practices promote salt and boron accumulation in soils on the Westside of Central California. The build-up of these inorganic salts leads to the degradation of arable land and reduction of crop production. Different clones of poplar trees have been shown to grow in salt- and boron-contaminated soils, and in doing so, gradually remove significant amounts of these inorganic salts from the soil. In an effort to identify molecular markers linked with salt and boron tolerance in poplars, 31 PCR primer pairs have been designed based on candidate genes identified from the literature, and an additional 31 PCR primer …

From The Laboratory To The Classroom: Characterization Of Microorganisms Residing On Mars-Based Spacecraft, Ryan Hendrickson

STAR Program Research Presentations

Planetary Protection is a discipline that aims to prevent forward biological contamination of other celestial bodies from Earth sources that might interfere with the search and detection of extraterrestrial life on interplanetary missions, such as Mars lander missions. Before any Mars mission with planetary protection bioburden requirements can be launched, the Planetary Protection team at JPL collects samples from the spacecraft and performs the NASA Standard Assay to detect any aerobic, mesophilic microorganisms. My primary objective for the summer was to take 60 isolates from the MER rovers and create long-term glycerol stocks, one working stock, and perform DNA extraction …

Testing Compounds As Sole Carbon Sources Of Strains Isolated From The Hot Lake Phototrophic Mat, Lyzbeth Becerra, Stephen Lindemann

STAR Program Research Presentations

Hot Lake is a meromictic, epsomitic lake. It has a phototrophic microbial mat that reassembles every year. The mat community is subjected to large fluctuations of salinity and solar irradiance. PNNL is interested in studying how these communities respond to the fluctuating environment conditions. To date, 70 isolates have been obtained from Hot Lake with the intention of characterizing their physiology to gain a better understanding of how they are naturally functioning in the Hot Lake mat community. I tested eight of these isolates in media designed to mimic the chemistry of Hot Lake with a selection of sugars, amino …

Creating A Package In R, Brit Schneiders, Eric Archer

STAR Program Research Presentations

In a time of increasingly efficient technology and data production, scientists are producing data faster than it can be analyzed. Therefore, user accessibility to data analysis is becoming more and more critical. In general, researchers have a set of raw data and want an efficient means to their final analysis. A package serves as that means by creating a set of functions and making them accessible to the user. Often, a user has a small piece of code to run (a single R script, for example), and that script requires the use of certain functions, which are contained in a …

Jellyfish Identification And Quantification In The San Francisco Estuary, Amalia Borson, Lindsay L. Sullivan, Wim Kimmerer

STAR Program Research Presentations

As potential predators and competitors of plankton-eating fish, jellyfish have the potential to negatively impact fish populations. Jellyfish were collected weekly with plankton tows from the RombergTiburonCenterpier in Tiburon, CA. Since some jellyfish were too small to identify, one tow was collected and preserved to record abundances, and a second tow was collected to rear jellyfish until distinguishing characteristics were visible enough for identification. Jellyfish in the preserved tows were then identified, measured, and counted, and their abundance (number m^-3) was calculated. Jellyfish from the second tows were reared in plastic buckets that were lightly bubbled using aquarium …

Preservation Of Biosignature Molecules In Potential Sample Return Container Of The Mars 2020 Mission, Kimberly E. Lykens, Fei Chen Ph.D

STAR Program Research Presentations

Preservation of Biosignature Molecules in Potential Sample Return Container of the Mars 2020 Mission

Kimberly Lykens¹ and Fei Chen²

¹Wittenberg University, Springfield, Ohio 45501 ²Jet Propulsion Laboratory, Pasadena, California, 91109

One requirement for sustainable life on terrestrial planets includes the presence of organic polymers, compounds that are essential for major biological functions such as replication and catalysis. An identified goal of the Mars mission in the year 2020 is to implement a sample-return to identify and validate signs of life on Mars through the discovery of biosignature molecules in Martian core samples. Martian core samples recovered …

Characterization Of The Drilling Via The Vibration Augmenter Of Rotary-Drills And Sound Signal Processing Of Impacted Pipe As A Potential Water Height Assessment Tool, Nicholas Morris

STAR Program Research Presentations

The focus of the internship has been on two topics: a) Characterize the drilling performance of a novel percussive augmenter – this drill was developed by the JPL’s Advanced Technologies Group and its performance was characterized; and b) Examine the feasibility of striking a pipe as a means of assessing the water height inside the pipe. The purpose of this investigation is to examine the possibility of using a simple method of applying impacts to a pipe wall and determining the water height from the sonic characteristic differences including damping, resonance frequencies, etc. Due to multiple variables that are relevant …

Behavioral Variation Between Two Clades Of Leptasterias Spp., Ashley N. Contreras

STAR Program Research Presentations

Leptasterias spp. are six-rayed sea stars found along the rocky intertidal of the northeast Pacific Alaska to Santa Catalina Island, southern California. In central California, three clades of Leptasterias are found in separate or mixed populations, in diverse habitats that range from shallow pools of seagrass and algae to bare rock exposed to crashing waves. Initial field observations of two clades from different locations suggested that behavioral variation may relate to habitat differences among clades. To measure differences in activity, the righting response was timed at both field sites. As a result of behavioral variation observed in the field, more …

Behavioral Variation Between Two Clades Of Leptasterias Spp., Ashley Contreras, Sarah Cohen

STAR Program Research Presentations

Behavioral Variation Among Two Clades of Leptasterias spp.

Leptasterias spp. are six-rayed sea stars found along the rocky intertidal of the northeast Pacific Alaska to Santa Catalina Island, southern California. In central California, three clades of Leptasterias are found in separate or mixed populations, in diverse habitats that range from shallow pools of sea grass and algae to bare rock exposed to crashing waves. Two clades of Leptasterias were collected, Y-Clade from Mile Rock, San Francisco and L. aequalis from Pigeon Point, San Mateo to test for behavioral variation that may relate to habitat differences among clades. To measure differences …

Temporal Variation In Larval Release In Botrylloides Violaceous, Damion J. Delton, Sarah Cohen

STAR Program Research Presentations

Despite Botrylloides violaceous being a globally invasive species in temperate marine habitats, little is known about it’s natural dispersal behavior. Previous work (Delton et al, 2011) suggests behavioral inferences based on a related and much more commonly studied species may not be appropriate, potentially due to a large size difference between the non-feeding larvae of these two species. Here, we consider factors that may affect dispersal potential. To study how light affects larval release, we measured timing of larval release in the field and compared larval size and time of release. Colonies were collected from floating docks in Richmond, CA …

Estimating Genetic Diversity Of The Invasive Tunicate Didemnum Vexillum In Global Populations, Elizabeth S. Gutierrez, Sarah Cohen

STAR Program Research Presentations

Populations in Point Reyes National Seashore

The presence of the invasive tunicate Didemnum vexillum in the San Francisco Bay was first documented in 1993, and it has since been found in Point Reyes National Seashore. In 2012, 38 samples of D. vexillum were collected from two locations in Point Reyes: Tomales Bay and Drake’s Estero. This summer, DNA from the 38 samples was extracted and analyzed using microsatellite and mitochondrial markers. The goal of this analysis was to determine the level of genetic connectivity between these two populations and their relationship to other global populations in order to answer important …

Inter-Comparison Of Forward Radiative Transfer Computer Models, Jesse Venegas, Anthony Davis, Michael Garay, Olga Kalashnikova

STAR Program Research Presentations

Radiative Transfer Models for Aerosol Remote Sensing Signals with Polarization

This project is an inter-comparison task that began when the Jet Propulsion Laboratory (JPL) began moving into polarized (vector) radiative transfer. A number of codes existed and since then more have been developed. NASA has been developing new instrumentation to study aerosols from high-altitude aircraft, and eventually from satellites. The task at hand is to compare a total of eight physics-based computer models, contributed from researchers around the globe, to make sure the codes agree with one another across a set of cases that are representative of aerosols in the …

Depth To Bedrock Estimations Using The H/V Spectral Ratio In The San Joaquin Valley, Tyler A. Morelli

Natural Resources Management and Environmental Sciences

Basin depth is a fundamental parameter in many types of geologic studies such as resource exploration and regional tectonic mapping. The Horizontal-to-Vertical Spectral Ratio (“H/V”) method has been successfully applied to estimating of basin depths up to several hundred meters. In this study the H/V method is applied to the deep basins on the western side of the San Joaquin Valley to determine the thickness of the sedimentary layers and depth to bedrock. Microtremor data from Earthscope Seismic Array stations are ideal to test the accuracy of the H/V method on deeper basins because the data is of high-quality and …

The Effects Of Microfluidization And Homogenization On The Composition And Structure Of Liposomal Aggregates From Whey Buttermilk And Commercial Buttermilk, Tracey Mai T. Nguyen

Master's Theses

Milk derived ingredients from the production of cheese and butter can be used as vehicles for nutrients. Buttermilk is a nutritious product of milk that comes from the churning of cream into butter. One of the advantages of buttermilk is that it is enriched in milk fat globule components, such as phospholipids and forms emulsions with fat when treated with high shear. The objective of this work was to explore the effects of shear on regular buttermilk and whey buttermilk in terms of liposomal aggregate size and chemical composition. The effects of microfluidization at 2000 psi and homogenization at 2000 …

The Design And Fabrication Of An Electrostatically Actuated Diaphragm With A Silicon-On-Insulator Wafer, Elizabeth L. Brooks

Master's Theses

Electrostatically actuated silicon membranes were designed, modeled, fabricated, and characterized. The intended application was for use in a microspeaker. Fabrication issues necessitated the use of thick diaphragms with a large gap between the electrodes. The devices did not function as speakers but did show actuation with a high DC voltage. Device dimensions were chosen by examining membrane mechanics, testing the processing steps required for device fabrication, and modeling with COMSOL. Several adhesives were researched to fabricate the device sidewalls, including BCB, PMMA, and TRA-Bond F112. A method for patterning PMMA through photolithography was found using a scanning electron microscope. Masks …

Finite Element Models Of The Knee & Hip Joints: Using Opensim To Predict Muscle Forces, Kevin S. Jones, Spencer D. Wangerin, Jeffrey D. Pyle, Stephen M. Klisch, Scott J. Hazelwood

STAR Program Research Presentations

Quantitative data of stresses and strains in the cartilage of the knee and hip joints are required to design prostheses and can be used to give accurate advice to patients with cartilage damage as to which activities should be avoided. Instrumented hip implants can only give the overall resultant force in the joint, not the stresses and strains throughout the cartilage. Finite Element (FE) models of the Knee and Hip are being constructed in order to obtain the stresses and strains in articular (of the joint) cartilage. Muscle forces and joint contact forces are required as inputs to these FE …

Lithic Analysis Of Coyote Canyon Mammoth Site Sediments, Kaitlin M. Hill, George V. Last

STAR Program Research Presentations

The landforms and geologic layers of Southeastern Washington record fascinating, unique geologic events, including repeated catastrophic flooding that occurred during the last Ice Age. These floods left behind many distinctive features, and a variety of fossils. After discovery of mammoth-sized bones in 1999, and confirmation that more bones were still in place in 2008, the Coyote Canyon Mammoth Site near Kennewick, Washington was secured for research, and formal excavation began in 2010.

Previous research suggests that the remains are buried in Ice Age flood deposits, which are overlain by eolian sediments, and those in turn overlain by slope wash. We …

Tools And Methods To Optimize The Analysis Of Telescopic Performance Metrics On Sofia, Steven R. Wilson, Holger Jakob, Stefan Teufel, Zaheer Ali, Jeffrey Van Cleve, Brian Eney, Greg Perryman

STAR Program Research Presentations

SOFIA is an infrared observatory mounted on a modified 747 engineered to do infrared astronomy at 45000 feet. The telescope equipment contains a number of sensors and stabilizers that allow the telescope to capture images while mounted in a moving plane. We have developed methods to analyze the performance of the telescope assembly that will help improve the stabilization and image capturing performance of the observatory. Here we present reusable methods to analyze telescope performance data that will enable improvements in the quality of the scientific data that is produced by the SOFIA. This poster focuses on the multi-flight performance …

Feeding Ecology Of Delta Smelt During A Seasonal Pulse Of Turbidity, William A. Hilton, Aaron Johnson, Wim Kimmerer

STAR Program Research Presentations

The delta smelt (Hypomesus transpacificus) is a small, pelagic fish endemic to the San Francisco Estuary (SFE) and protected under federal and state endangered species acts. This study examines the diet of adult delta smelt during their spawning migration in the winters of 2010 and 2012. Delta smelt and their zooplankton prey were sampled concurrently during a seasonal pulse of turbidity at sites along their migratory route from the low salinity zone in Suisun Bay to the fresher waters of the Sacramento/San Joaquin Delta. Gut contents were identified to the lowest possible taxon and counted, along with zooplankton …

Feedback Stabilization At Spear3, Daniel Kelley, Jeff Corbett

STAR Program Research Presentations

The SPEAR3 synchrotron lightsource at SLAC relies on a sophisticated radio frequency (RF) timing system to maintain current – electrons – in the storage ring. One problem SPEAR3 operators have dealt with is the thermal expansion of one of the cables supporting this RF timing system. As the cable expands and contracts with the diurnal rise and fall of the sun, the phase of the RF in the cable shifts. This shifting phase affects the timing accuracy of electron injections into the storage ring.

A common feedback control algorithm PID (Proportional Integral Derivative) has countless applications in engineering. PID feedback …

Investigating Graph Clustering For The Power Grid, Gabriela Radu, Emilie Hogan

STAR Program Research Presentations

Given time series data from multiple power generators containing measurements of phase angle taken at many points in time. The goal is to cluster together generators which have similar phase angle behavior.

In order to achieve this goal, Fast Fourier Transforms and Euclidean distances were used to quantify similarities between generators. A graph was created in which two generators were connected if they were sufficiently similar (known as a nearest neighbor graph). Using different nearest neighbor values, matrices were generated based on similarities between generators. Specific time intervals were taken from the large data set to assess the time dependency …

The Standard New Astronomy Cryostat For Sofia: A Design For Cryogen-Free Infrared Astronomy, Carey F. Scott, Jeffrey Van Cleve, Eric Burgh, Earl T. Daley, Ali Kashani, Zaheer Ali

STAR Program Research Presentations

Astronomy at infrared wavelengths requires optical instruments that operate at low temperatures, which is typically done using liquid cryogens such as nitrogen and helium. These cryogens are costly and limit the operational time of the science instrument. The Standard New Astronomy Cryostat for SOFIA (SNACS) will provide a design for a helium cryocooler-cooled cryostat that meets the stringent airworthiness requirements of the Stratospheric Observatory for Infrared Astronomy (SOFIA) and can be used by future instrument builders to reduce the cost and risk of their instrument design and development. The SNACS dewar will provide approximately 3.4 x 10^-1 cubic meters …

Detection Of Salmonella Enterica, Escherichia Coli O157 And Listeria Monocytogenes Through Bead Based Magpix® Fluidics, Karamveer Singh, Francisco Fernandez, Sonam Sahni, Alejandro Calderón-Urrea

STAR Program Research Presentations

The research is testing the limits of detection and multiplexing with the use of three pathogens with the machine created from Luminex called Magpix. The pathogens are Salmonella enterica, Listeria monocytogenes, and Escherichia Coli O157. The gene that is being focused on for each pathogen is invA for Salmonella, hlyA for listeria, and hlyA for Escherichia Coli. In this research, we started with prepared probes that were attached to the magnetic microsphere beads. Then we extracted and amplify the DNA. Furthermore, the probes were combined to the amplified DNA and used this in Magpix which gave a corresponding MFI value.

International Space Station Organics Exposure In Orbit Experiment, Kegan W. Powers

STAR Program Research Presentations

ORganics Exposure in Orbit (OREOcube)

The OREOcube International Space Station experiment investigates [the] effects of solar and cosmic radiation on organic molecules in contact with inorganic substrates. The organic molecules and inorganic substrates were deposited as optically thin films on magnesium fluoride (MgF2) and aluminum oxide (Al2O3) windows. The organic samples include polycyclic aromatic hydrocarbons (PAHs), porphyrins and quinones. The inorganic substrates include titanium dioxide, a known photocatalyst. By measuring changes in the UV-Vis-NIR spectra of samples as a function of time while mounted on an external ISS platform, OREOcube measures, in real time, critical kinetic and mechanistic details of …

Program Performance Analysis For The Flight Opportunities Program, Kirsten M. Fogg, Ronald M. Young

STAR Program Research Presentations

The Flight Opportunities Program (FOP) provides access to relevant spaceflight environments to accelerate critical new technologies that will enable affordable access and future exploration of space. These relevant environments include frequent access to spaceflight altitudes, reduced gravity, and the ability for vertical takeoff and landing. The FOP provides access to technology demonstrations in relevant space-like environments by acquiring frequent flights on commercial suborbital reusable launch vehicles. A survey of program activities is being conducted to assess effectiveness of acceleration and maturation of new game-changing technologies. A portfolio of over 100 space-related technologies that have been accepted into the program’s payload …

Flitecam Data Process Validation, Jesse K. Tsai, Sachindev S. Shenoy, Brent Cedric Nicklas, Zaheer Ali, William T. Reach

STAR Program Research Presentations

FLITECAM Data Processing Validation

Many of the challenges that come from working with astronomical imaging arise from the reduction of raw data into scientifically meaningful data. First Light Infrared Test CAMera (FLITECAM) is an infrared camera operating in the 1.0–5.5 μm waveband on board SOFIA (Stratospheric Observatory For Infrared Astronomy). Due to the significant noise from the atmosphere and the camera itself, astronomers have developed many methods to reduce the effects of atmospheric and instrumental emission. The FLITECAM Data Reduction Program (FDRP) is a program, developed at SOFIA Science Center, subtracts darks, removes flats, and dithers images.

This project contains …

Solar Verification Project, Ariel Sitler, Christopher Clack

STAR Program Research Presentations

As we drain the Earth's fossil fuels, climate change increasingly puts pressure on scientists to find a cost-efficient renewable energy solution. A nation-wide system using weather resources already in place in combination with the construction of transmission lines and new electricity producing sites is proposed by mentor Chris Clack and associates. The model created optimizes this system with the projected electric load from 2006-2008. Real world publicly accessible solar data was collected and analyzed from ten sites across the contiguous U.S. Corresponding estimation data was computed by the model for solar and collected for comparison. The observation data had been …

Extending The Shelf Life Of Solid Rocket Motors Through Mechanical Activation Of, Emilio J. Cantu, Hope Klukovich, Timothy Haddad

STAR Program Research Presentations

A great deal of interest has been given to extending the life of our fleet of solid rocket motors. Once poured, these motors are essentially stored, unused, and are therefore subject to various environmental conditions. Cycling between high and low temperatures causes thermal expansion and contraction of motor materials. This will eventually lead to cracks forming and propagating in the rocket motor, rendering it unreliable and thus must be re-poured at great expense. A common binder material used in these motors is hydroxy-terminated polybutadiene (HTPB), which when cured with a diisocyanate, creates the solid matrix to house the energetic materials …

Characterization Of Bacillus Bacterial Strains; Optimum Growth Temperature, Proteolytic And Lipolytic Activity, Jamie Anne Boring

STAR Program Research Presentations

Characterization of Bacillus bacterial strains is an important step in identifying and removing biofilms contaminating processing equipment. These biofilms lead to large amounts of spores in the final product. Strains that produce spoilage enzymes cause off-flavors, structural defects and reduced shelf life of milk products. Using four treatments, we determined the best growth protocol for each strain to use in future experiments. Treatment 1: 80 ^o C heat shock and 32 ^o C 24hr incubation, Treatment 2: 80^o C/ 55 ^o C, Treatment 3: 100 ^o C/ 32 ^o C, Treatment 4: 100 ^o C/ 55 ^o C. Our …

Leatherback Hatchling Fitness, Violet Campbell, Shane Morales, Kelly Stewart

STAR Program Research Presentations

Leatherback hatchling fitness on land is essential for their success in entering the ocean without being predated. Two ways fitness on land may be measured are by how fast hatchlings crawl as well as how fast their righting responses are when they are flipped onto their carapaces. Hatchlings from certain nests have slower righting response times along with slower crawl times, both of which would increase the odds of predation and hinder the success of hatchlings reaching the ocean.

Hatchling crawling speed was determined by timing 12 randomly selected hatchlings from each nest to crawl a distance of two meters. …