Digital Commons Network^™

Determining Antimicrobial Activity In Hydrolysates Obtained From Buttermilk Substrates By Trypsin And Pepsin, Deborah Kim, Karen Ramun, Stephanie Carpintero, Suyapa Padillo, Antonio-Jose Trujillo, Rafael Jimenez-Flores

STAR Program Research Presentations

Milk proteins within their structure and sequence, contain peptides that exhibit antithrombotic, immunomodulatory, antimicrobial, antihypertensive, and other bioactivities. These peptides are released as the enzymes cleave milk proteins at certain locations of the amino acid chains. In this research, four different substrates were hydrolyzed with the gastrointestinal enzymes, pepsin and trypsin. The substrates were buttermilk (BM) and whey buttermilk (WBM), which are waste products of butter and cheese production, and supercritical CO₂ fluid (SFE) treated buttermilk (S-BM) and whey buttermilk (S-WBM). SFE acts as a solvent which removes triglycerides from the substrates and changes the overall composition of samples. …

Monitoring Abundance Of Carcinus Maenas Developmental Stages In Seadrift Lagoon, Andrea M. Fieber

STAR Program Research Presentations

Able to tolerate a wide range of habitats, temperatures, and salinities, Carcinus maenas thrives in much of its non-native range, out-competing native crabs for resources while diminishing food supplies for a variety of marine and estuarine organisms. In Seadrift Lagoon, a manmade inlet of Bolinas Lagoon in Marin County, California, green crabs comprise a significant portion of benthic fauna, and trapping efforts began in 2009 to eradicate them. We analyzed several years of trapping data to monitor demographic changes for this closed population, performed larvae and megalopae sampling with pot scrubbers, and counted eggs on ovigerous females captured in Seadrift …

Mathematically Modeling Synchrotron Radiation, Amy B. Knowles, Jeff Corbett

STAR Program Research Presentations

The Stanford Synchrotron Radiation Light Source accelerates electrons to relativistic speeds, creating an electron beam which emits radiation as it bends around the storage ring. The synchrotron radiation produced is valued by scientists for the high powered x-rays it gives off which allow them to study samples at the atomic and molecular level.

This project focuses on the mathematical modeling of synchrotron radiation using visible light. The current model used to characterize beam size at SSRL uses a Gaussian approximation for the radiation distribution, which is similar to but distinct from the Schwinger equations that are the theoretical model for …

Smooth Representation Of Functions On Non-Periodic Domains By Means Of The Fourier Continuation Method, Nicholas Rubel, David Bilyeu, Justin Koo

STAR Program Research Presentations

This report examines a new methodology in solving Partial Differential Equations (PDEs) numerically. The report also studies the accuracy of this new method as a PDE solver. This new Fourier Continuation (FC) method is one of a few that avoids the well-known Gibbs Phenomenon, which is the overestimation or underestimation of a function. These estimations are oscillations around a “jump” when a non-periodic function is expressed in terms of sines and cosines. Instead, the FC algorithm creates a smooth, periodic extension of a function over a general domain, as demonstrated by the many examples presented here. The FC algorithm was …

Characterization Of Putative Wnt3a-Inducible Enhancers, Katelynn C. Lee, Nicholas Hum, Aimy Sebastian, Gabriela Loots

STAR Program Research Presentations

The Wnt signaling pathway has been previously shown to play a major role in regulating bone metabolism and it is emerging as a target for the therapeutic intervention of bone thinning disorders such as osteoporosis. Several Wnt proteins have been shown to be expressed in bone and mutations in Wnt pathway members such as Wnt co-receptor Lrp5 and Wnt inhibitor Sost have been shown to be associated with low or high bone mass disorders, however, very little is known about specific roles played by different Wnt ligands in bone development, repair and remodeling. To identify downstream targets of Wnt signaling …

Identifying Glacial Movement Through Fluvial Sediment Analysis, Alexis D. Freeman

STAR Program Research Presentations

The primary purpose of this study is to better understand how climate affects river dynamics. It is also important to understand the relationship between glaciers and the transportation of sediments, as this allows us to study changes in transport through time. The studied sediments were gathered from outcrops located in Fresno Ca, off of Friant road. The origin of these sediments are the Sierra Nevada Mountains. Mesozoic metasedimentary and metavolcanic sediments are derived from the foothills, while the granitic sediments are derived from higher up in the mountains. The sediments are characterized by both their degree of rounding, and their …

Using Bromide Tracer To Measure Uranium Diffusivity In Ground Water Sediments, Francis Michael Tee, Morris E. Jones, Megan K. Dustin, Sharon Bone, John Bargar

STAR Program Research Presentations

More than 129 million liters of groundwater are contaminated with uranium at Old Rifle, Colorado – a former uranium-processing site that operated until 1958. The original Department of Energy (DOE) strategy for remediation, involving natural flushing of U from the groundwater through mixing with surface water, has not proven successful. Thin pockets of silt-, clay-, and organic-rich sediments referred to as naturally reduced zones (NRZs) act both as sinks and sources of U to the aquifer, contribute to plume persistence, and appear to be diffusion limited controlled.

To better understand how the NRZs are diffusion limited controlled, a bromide tracer …

Radiographic Density Of Selected Materials At Different Thicknesses, Thien Quang Vu-Nguyen, Bernice Mills

STAR Program Research Presentations

X-rays are widely used in medicine and materials science to identify impurities or fractures within the target object. In material science, x-rays can be used to identify the thickness of samples with different material densities. The current method is to collect a radiograph using estimated conditions, evaluate the results, then make changes to the conditions. The residual image can be rescanned making it possible to obtain an image of lower radiographic density. By tabulating or graphing the effects of energy changes and rescans, a more informed choice of conditions is possible.

For this study, six materials were chosen with a …

Radiocarbon Isotopic Classification Of Deep Tropical Forest Soils, Brooke Butler, Karis J. Mcfarlane, Jennifer Pett-Ridge, Katherine A. Heckman

STAR Program Research Presentations

Tropical forest soils have an important role in global carbon (C) stocks. Small changes in the cycling of C could drastically affect atmospheric carbon dioxide (CO2) concentrations and active cycling of carbon in a forest community. Currently, little is understood of how tropical forest soils will respond to the increasing global temperatures. To examine the effects of warming/ drought on losses of older versus younger soil C pools, we implemented radiocarbon (14C) isotopic characterization of various soil plot samples and depths from the Luquillo Experimental Forest, Puerto Rico. 14C was measured using Accelerated Mass Spectrometry (AMS) from catalytically condensed carbon …

Hypometabolic Stasis In Snails (Cornu Aspersum) For Adaptability And Survivability Under Extreme Environmental Conditions, Jamie Lyn D. Pacada, Lauren Mc Keown, Sadaf Esteaneh, Oscar L. Ramos, Yekaterina Popova, Yuri V. Griko

STAR Program Research Presentations

NASA’s endeavor to engage in long-haul space travel, requires a human defense system against harmful environments in space. Muscle atrophy and bone mass loss are documented effects of long term exposure to space flight environment and microgravity. Our aim is to approach an adaptation to extreme environments on the biological level, rather than simulate a terrestrial environment in space. A correlation has been shown between metabolic suppression and the protection of biological organisms from damaging effects of space environments. Certain bacteria and invertebrates have indicated a link between the reduction and suspension of metabolism, and surviving exposure to cosmic radiation. …

Resolving Emission Lines Of Sodiumlike Fe Xvi Using Ebit, Sandi Lavito

STAR Program Research Presentations

High resolution crystal spectrometers on sounding rockets and orbiting satellites, such as the Solar Maximum Mission, show strong X-ray emission from the n= 3 to 2 transitions in neon-like Fe XVII. Two of the strongest lines are the 3d to 2p resonance and inter combination lines at 15.01 Å (3C) and 15.26 Å (3D).

Intensity ratios of these solar lines range from ~ 1.6 to 2.8. The lower ratios are a result of a line from Na-like Fe XVI inner shell satellite line blending with the Fe XVII inter combination line, 3D. The wavelength of the Na-like line is not …

Designing A Remote Aerial System To Image And Analyze The Health Of Grape Crops At Cal Poly San Luis Obispo, Alexander D. Vice, Dylan Benton, Adipratnia Asmady, Andrew Mercier, Andrew Meyer, Eric Belfield, Aaron Drake

STAR Program Research Presentations

In the last decade the prevalence of unmanned aerial vehicles (UAVs) has exploded; however, the presence of UAVs in research situations is still a relatively new and untested field. The autonomous flight lab (AFL) at Cal Poly San Luis Obispo is a new lab site that is dedicated to using UAVs to benefit research for all backgrounds. Before AFL was able to fly missions we first needed to actually receive our vehicles, create a lab space that would provide all of the necessary resources and equipment necessary to fly, and to be granted permission by the Federal Aviation Administration (FAA) …

Directed Energy Deflection Laboratory Measurements, Travis Brashears, Philip Lubin, Gary B. Hughes, Peter Meinhold, Jonathan Suen, Payton Batliner, Caio Motta, Janelle Griswold, Miikka Kangas, Isabella Johansson, Yusuf Alnawakhtha, Kenyon Prater, Alex Lang, Jonathan Madajian

Statistics

We report on laboratory studies of the effectiveness of directed energy planetary defense as a part of the DE-STAR (Directed Energy System for Targeting of Asteroids and exploRation) program. DE-STAR and DE-STARLITE are directed energy "stand-off" and "stand-on" programs, respectively. These systems consist of a modular array of kilowatt-class lasers powered by photovoltaics, and are capable of heating a spot on the surface of an asteroid to the point of vaporization. Mass ejection, as a plume of evaporated material, creates a reactionary thrust capable of diverting the asteroid’s orbit. In a series of papers, we have developed a theoretical basis …

Simulations Of Directed Energy Thrust On Rotating Asteroids, Janelle Griswold, Jonathan Madajian, Isabella Johansson, Krysten Pfau, Philip Lubin, Gary B. Hughes, Aidan Gilkes, Peter Meinhold, Caio Motta, Travis Brashears, Qicheng Zhang

Statistics

Asteroids that threaten Earth could be deflected from their orbits using directed energy to vaporize the surface, as the ejected plume creates a reaction thrust that alters the asteroid’s trajectory. In this situation, a critical issue is the rotation of the asteroid relative to the directed energy beam, as this will reduce the average thrust magnitude and modify the thrust direction. Flux levels required to evaporate surface material depend on the surface material composition, rotation rate, albedo, and thermal and bulk mechanical properties of the asteroid. The observed distribution of asteroid rotation rates is used, along with an estimated range …

An Investigation Of The Effect Of Malathion On Adaptive Plasticity Of Pseudacris Sierra, Michael Jonathan Maples

Master's Theses

This thesis is composed of two chapters. Chapter one reviews what is known about adaptive plasticity in response to predators, describes the physiological systems involved in such plasticity, and outlines the evolutionary consequences of adaptive plasticity. Chapter two describes a scientific experiment that investigates how malathion may impact adaptive plasticity in the Sierran Treefrog, Pseudacris sierra. Anuran tadpoles suffer high mortality rates due to predation. In response to strong selective forces relating to these high predation rates, tadpoles evolved the ability to adaptively respond to predators through morphological and behavioral plasticity. The morphological and behavioral responses are varied and depend …

Identification And Abundance Of Jellyfish In The San Francisco Estuary, Carrie Fong

STAR Program Research Presentations

In 2010 the delta smelt (Hypomesus transpacificus), became protected under the California Endangered Species Act. Endemic to the San Francisco Estuary, their numbers have been declining since the mid 1990s. Many factors have contributed to their low numbers, such as alterations to their habitat, predation, water diversions, and prey abundance. Several invasive species of jellyfish may be sources of competition for the native delta smelt because they both eat the same food source of copepods. From July 2010 to December 2012, samples of zooplankton, including jellyfish, were taken from 9 different stations in the high and low salinity …

Wet Chemical Synthesis And Characterization Of Nanomaterials For Solar Cell Applications, Krystle N. Sy, Ramprasad Gandhiraman, Jessica E. Koehne

STAR Program Research Presentations

During long term space missions, it is necessary to have a reliable source of energy. Solar cells are an easy and reliable way to convert energy from the sun to electrical energy. NASA has used solar cells manufactured on Earth as an energy source for many of its missions. In order to develop technologies that will enable high efficiency solar cells, we are synthesizing nanostructured materials. A range of nanostructured materials, such as titanium dioxide nanowires, nickel nanoparticles, copper nanoparticles, and silver nanoparticles/nanowires, are synthesized. In this work, we are reporting on the synthesis of these nanomaterials and the electron …

Fortifying Glass, David Guo

STAR Program Research Presentations

Glass can be very fragile, and many techniques have appeared to try to resolve this problem. One technique that has been developed to fortify glass is the depositing of metal atoms into the glass. The specific term for this technique is called “doping.” By combining different metals at different composition ratios, different levels of glass fortifications can be achieved. A pump-probe is applied to the glass which expands and contracts. By shooting electrons at the glass films, an electron diffraction pattern is achieved which contains data from the contraction and expansion of the glass film. This is where this poster …

Design Of A Non-Contact Home Monitoring System For Audio Detection Of Infant Apnea, Daniel T. White

Master's Theses

Infant apnea is a widespread condition in which infants fail to effectively breathe, and can lead to death. Clinical solutions exist for continuous monitoring of respirations in a hospital setting and requiring constant skin contact. This thesis investigates the construction of a proof of concept device that performs in-home monitoring without skin contact and with commonly available off-the-shelf components.

The device constructed used a directional microphone to detect breathing sounds, an omnidirectional microphone to detect ambient noise as a baseline to help isolate the breathing sounds, and LabVIEW software deployed on an inexpensive laptop computer to quantify incidents of apparent …

A Concept Validation Of A Magnetometry-Based Technology For Detecting Concealed Weapons In Vehicle Door Panels, Nar Vang

Master's Theses

Acts of insurgency have become an increasing threat resulting in extensive measures being taken by the law enforcement authorities to mitigate their devastating effects on human life and infrastructure. This thesis introduces a magnetometry-based information, and signal processing methodology for detecting concealed ferrous objects in vehicle body panels. From extensive literature research, it was observed that while magnetic sensors have been used in a variety of related applications, but they have not been extensively applied to the on-road detection of firearms and explosives concealed in vehicles. This study utilized an extensive experimental protocol for preliminary concept validation. The main idea …

Composition Effects On Sheen And Spread Rate Of An Interior Flat, One Coat Latex Paint Formulation, Dana James Christensen Ii

Master's Theses

Interior flat, white latex paint is a common coating applied to walls around the world. Development of a coating with one coat hide capability is a pinnacle achievement for paint formulators as it has the potential to save consumers both time and money. One coat paints already exist on the market, but they are limited in color, coverage, and often have many disclaimers. Work done was part of a project initiated by ChemoursTM Titanium Technologies.

The goal of the project presented in this article was to create an interior flat, white latex paint that yields a spread rate of greater …

Yielding And Microstructure In A 2d Jammed Material Under Shear Deformation, Nathan C, Keim, Paulo E. Arratia

Physics

The question of how a disordered material's microstructure translates into macroscopic mechanical response is central to understanding and designing materials like pastes, foams and metallic glasses. Here, we examine a 2D soft jammed material under cyclic shear, imaging the structure of ∼5 × 10⁴ particles. Below a certain strain amplitude, the structure becomes conserved at long times, while above, it continually rearranges. We identify the boundary between these regimes as a yield strain, defined without rheological measurement. Its value is consistent with a simultaneous but independent measurement of yielding by stress-controlled bulk rheometry. While there are virtually no irreversible …

Poly(N-Isopropylacrylamide) Hydrogels For Storage And Delivery Of Reagents To Paper-Based Analytical Devices, Haydn T. Mitchell, Spencer Schultz, Philip Costanzo, Andres W. Martinez

Chemistry and Biochemistry

The thermally responsive hydrogel N,N'-methylenebisacrylamide-cross-linked poly(N-isopropylacrylamide) (PNIPAM) was developed and evaluated as a reagent storage and delivery system for microfluidic paper-based analytical devices (microPADs). PNIPAM was shown to successfully deliver multiple solutions to microPADs in specific sequences or simultaneously in laminar-flow configuration and was found to be suitable for delivering four classes of reagents to the devices: Small molecules, enzymes, antibodies and DNA. PNIPAM was also able to successfully deliver a series of standard glucose solutions to microPADs equipped to perform a colorimetric glucose assay. The results of these tests were used to produce an external calibration …

Towards Bioregenerative Life Support For Extended Human Exploration: Experiment Development For Testing The Fitness Of Algae In Space, Audrey Fry, R. Craig Everroad, Jessica E. Koehne, Thomas E. Murphy, Brad M. Bebout

STAR Program Research Presentations

Microbes such as algae and bacteria are promising candidates for supporting extended human space travel, as they are robust sources of food, fuel, waste cycling, and oxygen production. Growing microbes on membranes reduces the mass and water demands of a bioreactor system, both of which are important considerations in space travel. The European Modular Cultivation System (EMCS) on the International Space Station (ISS) provides an ideal opportunity to test the effects of microgravity and other aspects of the space environment on algal growth on membranes. This project aims to find optimal growing conditions and measurement technologies that conform to the …

How To Save The World: One Life (And Uav) At A Time!, Natalie A. Wright

STAR Program Research Presentations

The proliferation of Uninhabited Aerial Vehicles (UAV’s) in commercial and public service aviation arenas has begun to gain the attention of many citizens. Popularized vehicles such as quadcopters have applications in aerial photography, small package delivery and hobbyist/consumer interests. Use of small-scale, fixed- and rotary-wing, remotely operated systems has been popularized as a result of such vehicles’ size and operational simplicity, which widens the range of application of this technology. Public service applications have the potential to advance emergency services management, which can substantially contribute to the public wellbeing. This research will examine the utility of multi-rotor systems in the …

Eating On Campus: Assessing The Nutrition Environment For Students, Kelsey Degreef, Madison Fishler, Rachel Gipson, Kelly Koyano, Jansen Lei

Baker/Koob Endowments Awarded Projects

The food environment is increasingly thought to have a major influence on eating behavior. University campuses offer a unique opportunity to study the extent to which food environments influence eating, but such studies must be based on reliable measures. The objective of this project is to use a standard and reliable instrument, the Nutrition Environment Measures Survey (NEMS), to describe dining venues on the Cal Poly campus, and to compare Cal Poly food stores with off-campus alternatives. The five student investigators on this multidisciplinary, team-­based project will undergo training to use standard protocols to apply the NEMS to 18 campus …

Development Of An Interactive Wave Drag Capability For The Openvsp Parametric Geometry Tool, Michael Jon Waddington

Master's Theses

Minimizing wave drag is critical to successful and efficient transonic and supersonic flight. Area-ruling is the process of managing the cross-sectional area of an aircraft to lessen the wave drag experienced in flight. Effectively calculating the necessary areas for a given aircraft can be difficult, and existing tools for conducting a wave drag analysis often carry limitations in both functionality and availability.

In this work, the author utilized an existing parametric geometry tool named OpenVSP to create an interactive design tool for approximating zero-lift wave drag. Here, the wave drag calculation methodology used in industry for decades is combined with …

A Lego® Brewster Angle Microscope For Quantitative Monolayer Film Analysis, Nicholas Benz

Physics

In order to study single-molecule thick films and their phase behavior we built a Brewster Angle Microscope (BAM). BAM’s are inherently expensive due to their accuracy and precision. We built a fully functional BAM using Lego® Mindstorm® kits for the fraction of the price of a commercial BAM. And by utilizing the 10µm patented Lego® tolerance, comparable accuracy was attained. The BAM was mounted to a Langmuir-trough and will be used for laboratory experiments for optics and physical chemistry along with research on lung surfactant and on liquid crystals.

Enhanced Presentation Of Tomographic Data, Carmen Watts Clayton, Bernice Mills, George Beffleben, Thien Vu-Nguyen

STAR Program Research Presentations

X-ray tomography yields a very large amount of data in three dimensions. Effectively displaying this data to a broad audience is a challenge. Techniques are discussed to improve presentation of movies of both 2D and 3D tomographic data using commercially available softwares.

Measuring Blood Flow In The Skeletal Muscle Microcirculation Using Laser Speckle Flowmetry, Kenneth Howard Gouin Iii

Biomedical Engineering

The presence of a native collateral circulation, which serves as a natural bypass for blood flow around an occlusion, improves prognosis for patients with ischemic diseases, such as peripheral arterial occlusive disease (PAOD). However, not all patients have a native collateral circulation, and animal models suggest a genetic basis for this variability. In mice, such as the BALB/c, that lack native arteriolar collaterals, neocollateral formation from capillaries that connect two arterial trees can occur after arterial occlusion, resulting in reperfusion of the ischemic watershed. Immature arterialized collateral capillaries (ACCs) at 7 days post arterial occlusion do not vasodilate in response …