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California Polytechnic State University, San Luis Obispo

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Executive Committee - Meeting Minutes, 9/27/2016, Academic Senate Sep 2016

Executive Committee - Meeting Minutes, 9/27/2016, Academic Senate

Academic Senate Minutes

No abstract provided.


Optimizing Gdna Purification And Qpcr Techniques For Specific Detection And Enumeration Of Probiotic Bacteria, Katelynn C. Lee, Jennifer Vanderkelen, Christopher Kitts Sep 2016

Optimizing Gdna Purification And Qpcr Techniques For Specific Detection And Enumeration Of Probiotic Bacteria, Katelynn C. Lee, Jennifer Vanderkelen, Christopher Kitts

STAR Program Research Presentations

Probiotics are live microorganisms, like bacteria and yeast, which are thought to provide health benefits when ingested. Large manufacturers produce blends of probiotics for supplementing the diets of agriculturally important animals. Commercial probiotic products are labeled with the number of cells present at the time of manufacture. Our assignment was to develop an assay which can be used to quantify the number of specific lactic acid bacteria present in certain probiotic products, thus verifying the number reported on the product labels. This quantification process involves isolating genomic DNA (gDNA) from the given samples and then running the DNA through a …


Post-Liquefaction Residual Strength Assessment Of The Las Palmas, Chile Tailings Failure, Tristan Reyes Gebhart Sep 2016

Post-Liquefaction Residual Strength Assessment Of The Las Palmas, Chile Tailings Failure, Tristan Reyes Gebhart

Master's Theses

Assessment of post-liquefaction residual strength is needed for the development of empirically-based, predictive correlations for earthquake engineering design. Previous practice commonly assigned negligible strengths to liquefied materials for engineering analysis, producing overly-conservative designs. Increasingly available case history data, and improved analytical tools have allowed for more accurate and less overly-conservative estimation of soil residual strength, improving empirical predictive models. This study provides a new case history to the limited suite of (approximately 30) liquefaction failure case histories available for post-liquefaction in-situ strength predictive correlations.

This case history documents the Las Palmas gold mine tailings dam failure, resulting from seismic-induced liquefaction …


Physically Effective Fiber Threshold, Apparent Digestibility, And Novel Fecal Microbiome Identification Of The Leopard Tortoise (Stigmochelys Pardalis), Breanna Paige Modica Sep 2016

Physically Effective Fiber Threshold, Apparent Digestibility, And Novel Fecal Microbiome Identification Of The Leopard Tortoise (Stigmochelys Pardalis), Breanna Paige Modica

Master's Theses

Particle size distribution of diet, feces, and change from diet to feces, as well as apparent digestibility (aDig, %) of selected nutrients, and novel fecal microbiome identification of mature female leopard tortoises (Stigmochelys pardalis, n = 16) fed exclusively one of three, nutritionally complete, pelleted diets were evaluated in a blind, complete randomized design study. Two diets included insoluble fiber (powdered cellulose) consisting of either 2.0 mm or 0.2 mm length. Insoluble fiber provides nutritional and physical benefits to both the animal host and the microorganisms that inhabit the gastrointestinal tract. Insoluble fiber length was used as a …


A 3-D Multiplex Paper-Microfluidic Platform, Mitchell Patrick Young Sep 2016

A 3-D Multiplex Paper-Microfluidic Platform, Mitchell Patrick Young

Master's Theses

3-D paper-based microfluidic devices (micoPADs) are small and portable devices made out of paper that offer a promising platform for diagnostic applications outside of a laboratory. These devices are easy to use, low cost, require no power source, and capable of detecting multiple targets simultaneously. The work in this thesis demonstrated the ability of a 3-D paper-microfluidic platform to simultaneously detect 5 targets. Rubber cord stock was used in conjunction with an acrylic housing unit to apply pressure along the edge of the channel. The indirect pressure application was successful in promoting vertical fluid flow between layers. Average channel development …


Review Of The Moral Complexities Of Eating Meat, Andy Lamey Aug 2016

Review Of The Moral Complexities Of Eating Meat, Andy Lamey

Between the Species

No abstract provided.


How To Become A Post-Dog. Animals In Transhumanism, Michael Hauskeller Aug 2016

How To Become A Post-Dog. Animals In Transhumanism, Michael Hauskeller

Between the Species

This paper analyses and deconstructs the transhumanist commitment to animal rights and the well-being of all sentient beings. Some transhumanists have argued that such a commitment entails a moral imperative to help non-human animals overcome their biological limitations by enhancing their cognitive abilities and generally “uplifting” them to a more human-like existence. I argue that the transhumanist approach to animal welfare ultimately aims at the destruction of the animal as an animal. By seeking to make animals more like us the freedom to live their life as the kind of creature they are is being denied to them. It is …


Optimization Of A Genomic Editing System Using Crispr/Cas9-Induced Site-Specific Gene Integration, Jillian L. Mccool Ms., Nick Hum, Gabriela G. Loots Aug 2016

Optimization Of A Genomic Editing System Using Crispr/Cas9-Induced Site-Specific Gene Integration, Jillian L. Mccool Ms., Nick Hum, Gabriela G. Loots

STAR Program Research Presentations

The CRISPR-Cas system is an adaptive immune system found in bacteria which helps protect against the invasion of other microorganisms. This system induces double stranded breaks at precise genomic loci (1) in which repairs are initiated and insertions of a target are completed in the process. This mechanism can be used in eukaryotic cells in combination with sgRNAs (1) as a tool for genome editing. By using this CRISPR-Cas system, in addition to the “safe harbor locus,” ROSAβ26, the incorporation of a target gene into a site that is not susceptible to gene silencing effects can be achieved through few …


Reverse-Engineering The Brain: The Parts Are As Complex As The Whole., Jens G. Pohl Aug 2016

Reverse-Engineering The Brain: The Parts Are As Complex As The Whole., Jens G. Pohl

Collaborative Agent Design (CAD) Research Center

The purpose of this paper is to review the current state of neuroscience research with a focus on what has been achieved to date in unraveling the mysteries of brain operations, major research initiatives, fundamental challenges, and potentially realizable objectives. General research approaches aimed at constructing a wiring diagram of the brain (i.e., connectome), determining how the brain encodes and computes information, and whole brain simulation attempts are reviewed in terms of strategies employed and difficulties encountered. While promising advances have been made during the past 50 years due to electron microscopy, the development of new experimental methods, and the …


Data Development And Analysis Pathways For Marine Mammals And Turtles: Creating A User Interface, Sarina Fernandez, Warren Asfazadour, Eric Archer, Lisa Komoroske Aug 2016

Data Development And Analysis Pathways For Marine Mammals And Turtles: Creating A User Interface, Sarina Fernandez, Warren Asfazadour, Eric Archer, Lisa Komoroske

STAR Program Research Presentations

A major obstacle in genetic research is developing streamlined methods for analyzing large amounts of data. The statistical computer programming language R provides users with the ability to develop packages containing specific functions in order to create more accessible data analysis pipelines. However, writing code in R can still be intimidating to those with little to no coding experience. Fortunately, the R package shiny provides a framework for developing web applications based on R functions. Using shiny, we developed a user-friendly web application containing functions of the R package strataG. The strataG package contains several functions for summarizing genetic data …


Comparative Analysis Of In Situ Fibronectin Using Tof-Sims, Spi-Ms, And Dropdesi-Ms In A Microfluidic Reactor, Shannon Fasing, Xiao-Ying Yu, Juan Yao, Jiachao Yu Aug 2016

Comparative Analysis Of In Situ Fibronectin Using Tof-Sims, Spi-Ms, And Dropdesi-Ms In A Microfluidic Reactor, Shannon Fasing, Xiao-Ying Yu, Juan Yao, Jiachao Yu

STAR Program Research Presentations

Fibronectin is an important biomolecule due to its role in cell differentiation, growth, kinesis, and adhesion. Such biological responses are mediated through membrane recognition and signaling; where fibronectin is found. Studying the outer molecular surface of fibronectin allows deeper insight into the microbiological reactions that occur during these processes. In situ mass spectrometry analysis in aqueous solution accurately represents fibronectin’s chemical components, made possible by a vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface). SALVI was paired with the analytical tools: time-of-flight secondary ion mass spectrometer (ToF-SIMS), single photon ionization mass spectrometer (SPI-MS) and drop …


Investigating The Infection And Persistence Of Sindbis Virus In Host Neurons, Gavin Schroter, Sally Hall, Monica Borucki Aug 2016

Investigating The Infection And Persistence Of Sindbis Virus In Host Neurons, Gavin Schroter, Sally Hall, Monica Borucki

STAR Program Research Presentations

Sindbis virus, an Alphavirus in the Togaviridae family, is an enveloped, single-stranded positive-sense RNA virus. Found mostly in parts of Africa, Australia, Egypt, Philippines, and Northern Europe – it is known to cause Ockelbo or Pogosta disease [1]. This disease is characterized by the sudden onset of fever, headache, and arthralgia; followed by arthritis, rash, fatigue, and muscle pain. The symptoms are gone within 14 days, though cases have shown joint pain to last from 12 months to 2 and a half years [4]. Common to several other viruses, Sindbis is transmitted from birds (its reservoir) to humans via an …


Inducing Emotional Response In Interactive Media, Keenan M. Reimer Aug 2016

Inducing Emotional Response In Interactive Media, Keenan M. Reimer

Master's Theses

Video games, and entertainment media have been developed for many years, and eliciting emotional experiences is an integral part of that process. Producing and editing game content in order to affect desired emotional experiences can be expensive and cumbersome to developers. This paper presents a study intended to show that such experiences can be affected with simple after-the-fact audio-visual effects. As subjects of the study, participants experience three different emotional states, fear, peace, and none, over three rounds and in three different game environments. They are given a simple narrative in each environment that directs them to gather various objects. …


Media Bias: How Much Does It Hurt Or Help The Media-Public Relationship?, Aidan Mathews Aug 2016

Media Bias: How Much Does It Hurt Or Help The Media-Public Relationship?, Aidan Mathews

Journalism

The focus of this research paper is to qualify the relationship between the public and the Media and to discover how much Media bias, or the perception of Media bias affects that relationship. An important angle taken in this study is the question of whether or not bias on the part of the Media actually helps the public when forming educated opinions.

The review of existing literature on Media bias and public perception of the Media and the collection of data via interviews with expert, professional journalists have led to two conclusions about the Media-public relationship: (1) in order for …


The Effects Of 4-Nonylphenol On The Immune Response Of The Pacific Oyster, Crassostrea Gigas, Following Bacterial Infection (Vibrio Campbellii), Courtney Hart Aug 2016

The Effects Of 4-Nonylphenol On The Immune Response Of The Pacific Oyster, Crassostrea Gigas, Following Bacterial Infection (Vibrio Campbellii), Courtney Hart

Master's Theses

Endocrine disrupting chemicals (EDCs) are compounds that can interfere with hormone signaling pathways and are now recognized as pervasive in estuarine and marine waters. One prevalent EDC in California’s coastal waters is the xenoestrogen 4-nonylphenol (4-NP), which has been shown to impair reproduction, development, growth, and in some cases immune function of marine invertebrates. To further investigate effects of 4-NP on marine invertebrate immune function we measured total hemocyte counts (THC), relative transcript abundance of immune-relevant genes, and lysozyme activity in Pacific oysters (Crassostrea gigas) following bacterial infection. To quantify these effects we exposed oysters to dissolved phase …


Effects Of Experimental Noise Exposure On Songbird Nesting Behaviors And Nest Success, Tracy I. Mulholland Aug 2016

Effects Of Experimental Noise Exposure On Songbird Nesting Behaviors And Nest Success, Tracy I. Mulholland

Master's Theses

Anthropogenic noise is an increasingly prevalent global disturbance. Animals that rely on the acoustical environment, such as songbirds, are especially vulnerable to these sounds. Traffic noise, in particular, overlaps with the frequency range of songbirds, creating masking effects. We investigated the effects of chronic traffic noise on provisioning behaviors and breeding success of nesting western bluebirds (Sialia mexicana) and ash-throated flycatchers (Myiarchus cinerascens). Because anthropogenic noise exposure has the potential to interrupt parent-offspring communication and alter vigilance behaviors, we predicted that traffic noise would lead to changes in provisioning behaviors, such as fewer visits to the …


The Preservation And Protection Of Native Biodiversity In The Guadalupe Nipomo Dunes Complex, Lindsey M. Whitaker, Matthew Ritter, Scott J. Steinmaus, Jonathan Hall Aug 2016

The Preservation And Protection Of Native Biodiversity In The Guadalupe Nipomo Dunes Complex, Lindsey M. Whitaker, Matthew Ritter, Scott J. Steinmaus, Jonathan Hall

Master's Theses

The Guadalupe Nipomo Dunes Complex (GNDC) is located within the California Floristic Province, a biodiversity hotspot characterized by high rates of endemism and exceptional loss of habitat. In 1980, the US Fish and Wildlife Service described the GNDC as, “the most unique and fragile ecosystem in the State of California,” and ranked it first on a list of 49 habitat areas needing state protection. It is the largest coastal dune area in California and it is one of the last remaining, relatively intact ecosystems of its type and size in the western United States. The growing recognition of species decline …


The Proteomic Response Of Gill Tissue In Tidally And Subtidally-Acclimated California Mussels, Mytilus Californianus, To Acute Emersion-Induced Anoxia, Aubrie N. Fowler, Lars Tomanek Aug 2016

The Proteomic Response Of Gill Tissue In Tidally And Subtidally-Acclimated California Mussels, Mytilus Californianus, To Acute Emersion-Induced Anoxia, Aubrie N. Fowler, Lars Tomanek

Master's Theses

Intertidal mussels regularly experience emersion-induced anoxia, in contrast to normoxic conditions experienced during submersion. We therefore hypothesized that acclimation to a tidal rhythm, as opposed to a rhythm of constant submersion, preconditions the proteome of the California mussel, Mytilus californianus, to respond differently to emersion-induced anoxia. Following acclimation, mussels either continued to receive the acclimation conditions (control) or were exposed to 100% nitrogengas (anoxia) during aerial emersion. We collected gill tissue for subsequent analysis of protein abundance with 2D gel electrophoresis and protein identification with tandem mass spectrometry. Relative to subtidally-acclimated mussels, tidally-acclimated mussels showed a greater propensity to …


Functional Vasodilation And Vascular Reactivity In Arteriolar Collaterals In The Spinotrapezius Of Males And Females, Britta C. Nelson Aug 2016

Functional Vasodilation And Vascular Reactivity In Arteriolar Collaterals In The Spinotrapezius Of Males And Females, Britta C. Nelson

Biomedical Engineering

Peripheral arterial occlusive disease (PAOD) occurs when there is narrowing or blockage of the peripheral arteries that carry blood to the extremities, most commonly the legs. The most common symptom of PAOD is intermittent claudication, or ischemic pain during exercise. Women with PAOD experience a greater extremity functional impairment than men. Since impaired vasodilation might cause the ischemic pain from PAOD, we should evaluate vasodilation post ligation in males and females in collateral vessels, which connect two arterial segments to maintain blood flow to an otherwise hypoxic area. First, we need to examine collateral vasodilation in unoperated male and female …


Review Of Bug Music: How Insects Gave Us Rhythm And Noise, Jonathan L. Friedmann Jul 2016

Review Of Bug Music: How Insects Gave Us Rhythm And Noise, Jonathan L. Friedmann

Between the Species

Review of Bug Music: How Insects Gave Us Rhythm and Noise, by David Rothenberg. This book proposes that the human sense of rhythm derived in part from insect sounds.


Carnivorous Companions And The Vegetarian’S Dilemma, Patrick J. Clipsham Jul 2016

Carnivorous Companions And The Vegetarian’S Dilemma, Patrick J. Clipsham

Between the Species

This paper is concerned with a problem that arises within ethical frameworks that imply that it is wrong for humans to consume meat or other animal products when vegan alternatives are available. The specific problem relates to the ethical difficulties associated with beginning a relationship with a companion animal that may require at least some animal-based foods in order to survive. I follow some psychologists in referring to the ethical problems associated with such companionship as the Vegetarian’s Dilemma. After approaching this dilemma from the perspective the animal rights approach and welfarist consequentialism, I argue that some important insights can …


Automated Detection Of Deep-Sea Animals, Dallas J. Hollis, Duane Edgington, Danelle Cline Jul 2016

Automated Detection Of Deep-Sea Animals, Dallas J. Hollis, Duane Edgington, Danelle Cline

STAR Program Research Presentations

The Monterey Bay Aquarium Research Institute routinely deploys remotely operated underwater vehicles equipped with high definition cameras for use in scientific studies. Utilizing a video collection of over 22,000 hours and the Video Annotation and Reference System, we have set out to automate the detection and classification of deep-sea animals. This paper serves to explore the pitfalls of automation and suggest possible solutions to automated detection in diverse ecosystems with varying field conditions. Detection was tested using a saliency-based neuromorphic selective attention algorithm. The animals that were not detected were then used to tune saliency parameters. Once objects are detected, …


Plant Growth Promotion By Bradyrhizobium Japonicum Under Heavy Metal Stress, M. Seneviratne, S. Gunaratne, T. Bandara, L. Weerasundara, Nishanta Rajakaruna, G. Seneviratne, M. Vithanage Jul 2016

Plant Growth Promotion By Bradyrhizobium Japonicum Under Heavy Metal Stress, M. Seneviratne, S. Gunaratne, T. Bandara, L. Weerasundara, Nishanta Rajakaruna, G. Seneviratne, M. Vithanage

Biological Sciences

The increase in usage of heavy metals in different industrial activities causes their existence in effluents. Excessive concentrations of these heavy metals pollute soil and water. Heavy metals cause toxicities and other harmful effects not only in humans and animals but also in plants and soil microorganisms. Heavy metals disrupt many biochemical and physiological activities in bacteria, including growth, development, enzyme and hormone production. Indole acetic acid (IAA) is one of the most important hormones in plants, which is secreted by both bacteria and plants. The present study assessed the effects of Ni, Pb and Cu on the growth of …


The Effects Of A Uniformly Weighted Exercise Suit On Biomarkers Of Bone Turnover In Response To Aerobic Exercise In Postmenopausal Women With Low Bone Density, Haley Frances Terndrup, Alison K. Ventura, Todd Hagobian, Scott Hazelwood Jul 2016

The Effects Of A Uniformly Weighted Exercise Suit On Biomarkers Of Bone Turnover In Response To Aerobic Exercise In Postmenopausal Women With Low Bone Density, Haley Frances Terndrup, Alison K. Ventura, Todd Hagobian, Scott Hazelwood

Master's Theses

Current options for maintaining or slowing aging-related bone mineral density (BMD) loss in postmenopausal women primarily include pharmaceutical agents. More recently, physical activity and exercise have been suggested as highly effective, low cost alternatives. Weighted aerobic exercise, utilizing load carriage systems (LCS), is known to increase the gravitational forces impacting bone, creating a higher osteogenic stimulus than standard aerobic exercise. In response to the positive research on aerobic exercise with well-designed LCS, Dr. Lawrence Petrakis, MD, developed a unique 5.44 kg uniformly weighted exercise suit. This study aimed to examine the effects of the uniformly weighted exercise suit on serum …


The Owl, The Goldfish And The Bull - The Question Of The Animal And Romantic Poetry, Hui Zhang Jun 2016

The Owl, The Goldfish And The Bull - The Question Of The Animal And Romantic Poetry, Hui Zhang

Between the Species

This article argues that the representation of animals in Romantic poetry contributes to the contemporary philosophical and ethical discussion of the question of animals by providing a literary expression of the latter. Conversely, reading depictions of animals in Romantic poetry with their philosophical implications in mind throws light on the oppositions between different human groups, such as between Orientals and Occidentals, or between males and females, in Romantic poetry. These categories connect with each other in different ways in the works of three prominent Romantic poets: William Wordsworth, Lord Byron and Alexander Pushkin. Animals in their poetry reflect their views …


Influence Of Nanosilver Surface Charge On Cytotoxic Efficacy Against Cancer Cells, Elliot Frey Jun 2016

Influence Of Nanosilver Surface Charge On Cytotoxic Efficacy Against Cancer Cells, Elliot Frey

Baker/Koob Endowments Awarded Projects

Silver and silver-based products are known to cause cytotoxic effects to both microbes and eukaryotic cells. Because of this property, silver nanoparticles (AgNPs) are being studied for their potential in targeted tumor treatments. Previous studies with microbes suggest that AgNPs with cationic capping agents possess enhanced cytotoxicity by virtue of Coulombic attraction between the nanoparticle and the negatively-charged cell wall. Since animal cells possess similar negatively-charged plasma membranes, this research hypothesized that human cells would be more susceptible to positively-charged AgNPs than to negatively-charged AgNPs. To investigate this hypothesis, cancerous cervical cells (HeLa) and healthy fibroblast cells (3T3) were subjected …


Effects Of Oral Ingestion Of Bisphenol A On Type 2 Diabetes Risk Markers Ii. Project, Alyssa Bird, Dana Williams Jun 2016

Effects Of Oral Ingestion Of Bisphenol A On Type 2 Diabetes Risk Markers Ii. Project, Alyssa Bird, Dana Williams

Baker/Koob Endowments Awarded Projects

Eleven healthy adults (3 M, 8 F; 21.3 ± 0.8 yr; 24.0 ± 3.8 kg/m2) were block randomized to a single ingestion of placebo, low dose BPA (4 μg/kg-bw), or high dose BPA (50 μg/kg-bw) on separate days in a double-blinded, crossover fashion. Blood was drawn at baseline and repeatedly for 3 hours following each condition. Data collection has been completed for 11 subjects, and analyses of plasma BPA concentrations in these subjects have shown three distinct concentrations for high, low, and placebo conditions. Preliminary analyses of plasma insulin concentrations in 3 subjects appear to show differences in concentrations between …


From Downtown To The Inner Harbor: Baltimore's Sustainable Revitalization, Vicente Del Rio Jun 2016

From Downtown To The Inner Harbor: Baltimore's Sustainable Revitalization, Vicente Del Rio

Focus

The revitalization of Baltimore's Inner Harbor and waterfront is internationally recognized as a planning and urban design model. This successful story started in the late 1950s with the Charles Center redevelopment plan for the core of the Central Business Distric and its positive effects on the city's economy. In the first of a twopart article, Vicente del Rio writes about this early plan and its role in Baltimore's efforts towards a sustainable revitalization. Next FOCUS will feature his account of the plan for the Inner Harbor and beyond.


Fernjo Farms Case Study, Michelle Cheda, Luis Trujillo, Lauren Ott, Andrew Lucchesi Jun 2016

Fernjo Farms Case Study, Michelle Cheda, Luis Trujillo, Lauren Ott, Andrew Lucchesi

Case Studies in Agribusiness

The history of Fernjo Farms is similar by many California dairy farms. It was started by a Portuguese immigrant from the Azores in the 1920s, who milked someone else’s cows for decades before he had saved enough to start his own farm. Today, Fernjo Farms milks 3,500 cows on three operations, and is expanding into permanent crops such as almonds and pistachios. The case explores the California dairy industry and challenges such as labor and regulations. Given current dairy costs and returns, Fernjo Dairy is considering other enterprises or moving.


Swine Handbook For Novice Ffa Members, Amelia Brown Jun 2016

Swine Handbook For Novice Ffa Members, Amelia Brown

Agricultural Education and Communication

No abstract provided.