Digital Commons Network^™

Diversity And Community Of Macroinvertebrates In Timpanogos Caves, Utah, Erika Frandsen, C. Riley Nelson

Library/Life Sciences Undergraduate Poster Competition 2024

The Timpanogos Cave System consists of three caves in American Fork, Utah. In 2003, the National Park Service (NPS) commissioned Dr. Riley Nelson and laboratory students to conduct a survey of the diversity and abundance of terrestrial arthropod species found within the caves. Over the two years they sampled in Timpanogos Caves, they identified 55 species of macroinvertebrates in an unpublished report. In the 20 years since the last sampling, many environmental factors could have changed in these caves, contributing to an overall decrease in both abundance and biodiversity of fauna. Therefore, we repeated the sampling done by the Nelson …

Distinguishing Leukemic Cells Using Fractal Chromatin Patterns And Machine Learning, Abigail Gordhamer, Ryan Cordner, Paul Young

Library/Life Sciences Undergraduate Poster Competition 2024

One of the most important tests in the clinical laboratory is the Complete Blood Count, which involves identifying the white blood cells in a patient’s blood. The respective counts of the different white blood cell types correlate with various states of health and disease and are critical to diagnosing diseases such as leukemia. Leukemic cells (blasts) are considered especially difficult to distinguish, and it is of the upmost importance that these cells are identified correctly. To aid in the process of leukemic cell identification, we quantified fractal patterns in the chromatin of white blood cells and used the data to …

Achievement Goals And Burnout In Pre-Health Profession Undergraduate Students, Hunter Nelson, Jamie Jensen

Library/Life Sciences Undergraduate Poster Competition 2024

Oral Presentation Honorable

Relevant Background

• STEM majors are saturated with Pre-health students

• There is high demand for academic excellence and high competition

• Medical school programs (Dyrbye, 2016) and health careers (Shanafelt et al., 2022) show a high rate of burnout

• Symptoms of burnout are mental fatigue, less selfsatisfaction, and depression (Koutsimani, 2021).

• Achievement Goal Theory (AGT) may predict burnout (Nadon et al., 2020).

A Survey Of Molecular Diagnostics Education, Kristina Berman, Ryan Cordner, Mary Davis

Library/Life Sciences Undergraduate Poster Competition 2024

Medical Laboratory Science (MLS) is vital for healthcare, involving the analysis of clinical samples to diagnose and monitor diseases. However, there's a scarcity of courses focused on molecular diagnostics, which could greatly benefit medical laboratory scientists.

Lake Bonneville's Legacy: Genetic Drift And Diversity Loss In Snake Range Bonneville Cutthroat Trout, Tanner Van Orden, Dennis Shiozawa, Peter Searle, Ana Kokkonen, Jonathan Reynolds, R. Paul Evans

Library/Life Sciences Undergraduate Poster Competition 2024

• Many Bonneville Basin streams have been isolated for 18,000-plus years (Behnke 1976).
• Bonneville cutthroat trout in small streams may have been experiencing genetic drift since the desiccation of Lake Bonneville.
• Small streams in the west desert of Utah and Nevada are at a high risk of genetic drift and diversity loss due to their small size

Effects Of Predation On Cognition In Brachyrhaphis Rhabdophora, Elizabeth F. Jenkins, Jerry B. Johnson

Library/Life Sciences Undergraduate Poster Competition 2024

RESEARCH QUESTION

Does “predation environment” affect the cognitive abilities of the tropical fish Brachyrhaphis rhabdophora?

Using Chimeric Autoantigen Receptor (Caar) T Cells To Eliminate Autoreactive B Cells In Autoimmune Diseases, Abigail Cheever, Chloe Kang, Hunter Lindsey, Mackenzie Hansen, Kim O'Neill, Scott Weber

Library/Life Sciences Undergraduate Poster Competition 2024

• Graves’ disease (GD) is an antibody-mediated autoimmune disease affecting the thyroid and causes hyperthyroidism
• 4th most common autoimmune disease in the US
• Autoreactive B cells produce anti-TSHR antibodies (TRAbs) which mimic thyroid stimulating hormone (TSH) and chronically stimulate thyroid cells causing hyperthyroidism
• There currently is no treatment which addresses the root cause of the disease

The Role Of Sirt7 In Beta Cell Function And Insulin Secretion, Trevor C. Kendrick, Jeffrey S. Tessem, Connor Littlefield

Library/Life Sciences Undergraduate Poster Competition 2024

Diabetes is a chronic metabolic disease characterized by an inability of beta cells to produce or secrete insulin due to decreasing beta cell mass, a condition induced by beta cell death or overuse. Current treatment consists of daily administration of insulin to diabetic individuals. We have shown that Sirtuin 7 (Sirt7), a deacetylase located in the nucleus, directly interacts with Nkx6.1, a transcription factor essential for beta cell function and proliferation. We have shown that one of the post translational modifications that impinges on Nkx6.1 activity is acetylation. Given Sirt7’s role as a deacetylase, and published reports demonstrating its impact …

Microbial Thiamine And Riboflavin Influence Drosophila Melanogaster Dietary Preference For Yeast, Dean B. Peterson, John M. Chaston

Library/Life Sciences Undergraduate Poster Competition 2024

Naturally prefer a yeast restricted diet over a full yeast diet

• Restricted – 50g yeast and 100g glucose (50g Y:100g G)
• Full – 100g yeast and 100g glucose per (100g Y:100g G)
• Microbes affect this dietary preference for yeast (DPY) through an unknown mechanism
• Common microbes studied with flies are acetic acid bacteria (AAB) and lactic acid bacteria (LAB)

Tardigrade Niche Partitioning, Samantha Hougaard, Byron Adams

Library/Life Sciences Undergraduate Poster Competition 2024

The two types of Tardigrade (also known as Tardigrade), Ramazzottius and Echiniscus, coexist in the same environment. It could be assumed that they partition their resources by food type to avoid competition. To test this theory, I limited the two tardigrade groups to one food source.

Effects Of Post-Translational Histone Modifications On Transcription Rate, Jacob Hunter, Steven Johnson, Aaron Bohn, Sarah Ricks, Sarah Hodson, David Bates, Steven M. Johnson

Library/Life Sciences Undergraduate Poster Competition 2024

DNA structure and organization in eukaryotic cells significantly impacts the regulation of geneexpression, cellular properties and overall behavior. At the most fundamental unit of this organization,~147 bp of DNA wraps 1.7 times around a histone octamer core, forming a collective unit called thenucleosome. Positioning and occupancy of nucleosomes around promoter elements of genes is knownto be a strong regulator of transcription in eukaryotic nuclei. Post-translational modifications (PTM’s) tothe protruding N-terminal tails of histone proteins are known to influence chromatin structure and thusgene expression; however, relatively little is known about the residual effect of histone PTM’s ontranscription rate.

Variation In Vital Rates Among Least Chub (Iotichthy Phlegethontis), Lauren Applebee, Mark C. Belk, Nathan St. Andre

Library/Life Sciences Undergraduate Poster Competition 2024

Conservation plans for a species often requires knowledge of population trends. These trends can be difficult to acquire, but one piece of information that can be attained from populations is the catch per unit effort (CPUE). We are trying to use CPUE in a new way to determine population demography for populations of Iotichthys phlegethontis, Least Chub.

Targeting Hsp90: A Novel Approach To Combat Synovial Sarcomas, Peyton Worley, Jared Barrott, Matt Kirkham, Drake Heithoff

Library/Life Sciences Undergraduate Poster Competition 2024

Synovial sarcomas are rare muscle cancers that have very few targeted therapies. HSP90 is a protein upregulated in this form of cancer and many others. A screening of this cancer was done in vitro, using FDA-approved drugs, showing that HSP90 could be a promising treatment option. A novel HSP90 inhibitor was applied to human and mouse synovial sarcoma cell lines and was analyzed after receiving a drug treatment for HSP90.

Determining If Optic Tecti Of Larval Zebrafish Receive Input From Deep Brain Photoreceptors, Emalie A. Christensen, Allison G. Pickens, Colby Odom, Griffin Sauer, Sarah Y. Jarrett, Maurice Hunt, Annalie Martin, Arminda Suli

Library/Life Sciences Undergraduate Poster Competition 2024

Deep brain photoreceptors (DBPs) are light-responsive sensory cells present in mammals and non-mammalian vertebrates. In non-mammalian vertebrates, studies have shown DBP involvement in various time and light dependent behaviors including circadian rhythm, phototaxis, and seasonal reproduction. In adult telosts, DBPs are present throughout the brain, including the optic tectum (OT), a midbrain structure that receives and integrates input from multiple sensory modalities. In this study, we aimed to determine if DBPs are part of or send input to the OT of larval zebrafish. Single-cell RNA sequencing of the larval zebrafish OT showed the presence of several opsins. We focused on …

Making Sense Of Messy Data: Classifying Metadata Variables In Public Transcriptomic (Rna) Data, Grace S. Brown, Tolulope Akinbo, Stephen Piccolo

Library/Life Sciences Undergraduate Poster Competition 2024

US researchers are required to deposit data in public repositories so other researchers can validate their findings and reuse their data for new studies. There are > 100,000 transcriptomic (RNA) datasets in the public domain. However, it is of little use without metadata (details about individual samples e.g. sex, race). Having quality metadata provides many benefits including

• controlling for confounding factors.
• improving precision medicine.

Research has suggested

• males are studied more than females.
• people of European descent are studied most.

We need to make representation more equitable so genetic research will be relevant to all people. The first step is characterizing …

Framing An Adversary: Ethnic Nationalism In The First Khmer Newspaper, Ian Lowman

The Thetean: A Student Journal for Scholarly Historical Writing

The study of nationalism examines both global patterns and the singleness of local response. In most fledgling nation-states, the modern phenomena of print and western capitalism transformed indifferent individuals into a politicized public. Printing houses profitably publicized and glorified vernaculars while propagating European ideas of state borders, national cultures, and patriotism. By employing this medium, nationalists toyed with ideologies specific to the concerns of their community; French nationalism thrived on an abstract battle against tyranny while Algerians couched their nationality in the struggle for freedom from France. Each nation's self-conception reflected a search for an ideal of commonality and a …

“Creating And Maintaining Black Life-Worlds”: The Black Aesthetics Of Bernardine Evaristo’S Blonde Roots And Girl, Woman, Other, Sharanya Dg

Criterion: A Journal of Literary Criticism

Black Aesthetics is the philosophical inquiry into the objects and practices of expressions coming from people who have been racialized as black. These expressive practices then lend to the creation of the life-worlds of people subjected to racist discourses. One such author in the contemporary English society, Bernadine Evaristo, responds to anti-black racist discourses by exploring the cultural plurality of British black life-worlds. This paper is a textual and formal analysis of two experimental novels of Evaristo to study how they distinctly present the quotidian lives of various characters in their racialised bodies to reflect on the sociocultural and political …

Effect Of Evening Vs. Morning Exercise On The Appetite Hormones, Penny Akre

Undergraduate Honors Theses

This paper explores the potential impact of exercise timing on appetite hormones and metabolic outcomes, aiming to enhance understanding of the complex interplay between exercise, appetite regulation, and weight management. A randomized trial involving premenopausal women investigated the effects of morning versus evening exercise sessions over an eight-week intervention period. Participants underwent comprehensive screening and a supervised exercise regimen, with pre-and post-training laboratory sessions assessing body composition and appetite hormone levels. Statistical analyses revealed no significant differences in appetite hormone levels between morning and evening exercise groups, but notable correlations were observed between appetite hormones and body composition measures. Despite …

How Fundamental Epistemic Limitation Impacts My Human Experience, Matthew Mcnairy

Undergraduate Honors Theses

My thesis examines different ways that I am ignorant through a philosophical lens. Through examining my ignorance, I gain insight into myself and the world. However, the kinds of ignorance that interest and irk me the most are fundamental to life. I explore two major sources of ignorance and reference many more. The first source of ignorance is myself. Myself includes my body, my emotions, and my actions. I can partially control each of these, but they also are not fully understood by me. I explore who I am as a self and what my moral responsibility is in the …

Effects Of Enzymatic And Chemical Bisulfite Conversion On Cell-Free Dna Fragmentation, Caroline G. Ploeger

Undergraduate Honors Theses

This project aims to investigate the DNA fragmentation produced by enzymatic bisulfite conversion compared to DNA fragmentation produced by traditional chemical bisulfite conversion techniques for the analysis of cytosine methylation in seminal cell-free DNA (cfDNA). Traditional chemical bisulfite (HSO₃^-) conversion methods are known to introduce biases and damage DNA, potentially compromising methylation analysis accuracy. In contrast, enzymatic conversion, utilizing biological molecules, is hypothesized to offer more precise visualization with reduced fragmentation (Lambert et al., 2019). While previous studies have analyzed methylation conversion efficiency in genomic DNA, this project specifically examines how these conversion techniques impact cfDNA.

20 …

Isothiocyanates In The Prevention Of Neural Tube Defects In Chick Embryos, Katrina Lantz

Undergraduate Honors Theses

Neural tube defects (NTDs), including anencephaly and spina bifida, contribute significantly to neonatal morbidity and mortality worldwide. Despite the widespread implementation of folic acid (FA) supplementation, its ineffectiveness in certain genetic populations, alongside the persistent prevalence of NTDs, underscores the need for alternative preventative strategies. This study investigates the potential of isopropyl isothiocyanate (IPI), a compound derived from Moringa oleifera (M. oleifera), in mitigating NTDs through the modulation of oxidative stress pathways. Using the chick embryo model, we aimed to determine whether IPI significantly reduces the incidence of chemically-induced NTDs by activating the Nrf2 cofactor, a key regulator of cellular …

Leaving Home: Loss And Restoration In Marilynne Robinson's "Gilead" And "Housekeeping", Zachary Stevenson

Undergraduate Honors Theses

The concepts of restoration and reconciliation are central to Marilynne Robinson’s first two novels, Housekeeping (1980) and Gilead (2004). Housekeeping structures itself around the narrator Ruth Stone’s thirst for familial wholeness, and Gilead tracks the aging Congregationalist minister John Ames’s growing realization that there are rifts in both his town and his personal life that demand healing. The books are also united in their ambivalence towards the possibility of achieving total wholeness, as in each novel the movement towards restoration is undercut in important ways. However, the books ultimately diverge in the scope of the restitution that they allow. Whereas …

Visual Thinking Strategies (Vts) In The Clinic: How The Humanities Affect The Way We Make Visual Diagnoses Within Medicine, Mitchell Smith

Undergraduate Honors Theses

Visual Thinking Strategies (VTS), a system of humanistic critical analysis that was developed in the 1990’s by Abigail Housen and Philip Yenawine, affords viewers of art the opportunity to have an increased level of interaction with artwork through the use of questions posed by an instructor. For viewers, such a process of critical analysis affords greater visual literacy. The same is arguably true of medical personnel who have trained to use said process in the making of medical observations and diagnoses. The purpose of this study is to understand if VTS has direct application within the realm of medicine, and …

The Role Of Age, Sex, And Mglur5 Receptors In Hippocampal Depotentiation In Mice, Michael Dew

Undergraduate Honors Theses

Synaptic plasticity of excitatory hippocampal circuits is one of the primary cellular mechanisms of learning and memory. Synaptic plasticity is also implicated in many disease states such as depression, post-traumatic stress disorder, and Alzheimer disease. Long-term potentiation (LTP) is the plastic strengthening of synapses, while long-term depression (LTD) is the weakening of synapses. Depotentiation is the process of reversing previously established long-term potentiation. Depotentiation has not been studied as thoroughly as long-term potentiation and long-term depression. We examined the role of age, metabotropic glutamate receptor 5 (mGluR5), and sex in the process of CA1 hippocampal depotentiation in young CD-1 mice …

Rapid Discovery Of House-Keeping Genes In The Bacterium Kushneria, Using A Slalom-Library-Based Crispri Forward Genetic Screen, Andrew Jenkins

Undergraduate Honors Theses

Rising soil salinity levels worldwide have resulted in the loss of millions of hectares of viable cropland. Several solutions to this problem have been posited, including looking into the properties of salt-tolerant plants. Recent research into salttolerant plants in southern Utah has found a host of microorganisms isolated from the soil, plant surfaces, or plant tissues that stimulate salt tolerance in alfalfa. Leveraging a Mobile-CRISPRi system to repress a library of genes created through the recently developed sgRNA Library Assembly by Ligation onto Magnetic Beads (SLALOM) protocol, we induced genome-wide repression of genes in Kushneria spp. grown under optimal and …

The Effects Of Diesel Exhaust Particle Exposure On Adipos Mitochondrial Bioenergetics And Inflammation, Cali Elizabeth Warren

Theses and Dissertations

Fine particulate matter (PM2.5) constitutes a significant component of ambient air pollution that has been implicated in the pathogenesis of metabolic disorders, including insulin resistance and type 2 diabetes. Among PM2.5 constituents, diesel exhaust particles (DEP) are prevalent particulates that infiltrate the bloodstream to drive systemic pathologies. The purpose of this study was to characterize the metabolic response of adipose tissue to DEP. We aimed to provide a comprehensive understanding by exploring mitochondrial bioenergetics, characterizing the inflammatory marker profile, including adipokines, and conducting a detailed histological analysis of adipocytes to provide valuable insights to the evolving understanding of the intricate …

The Mutation Of The Model Man: 1936-1945, Andrea Rassmussen

The Thetean: A Student Journal for Scholarly Historical Writing

Masculinity, or the ideal male model, differed significantly in the war years from the late 1930s. This evolution can be seen through articles in Coronet, in which the majority of stories had male heroes whose physical characteristics, personalities, and social graces all changed as the war started and progressed. The ideal man shifted from the Successful Businessman of the 30s to the Individualistic Team Player of the 40s. I chose these names because they encapsulate the contradiction that made up the model man of the war years. No more was the ideal a cutthroat businessman concerned with nothing except succeeding, …

Tractor Beams And Disease: Probing Repeat Rna Structure Through Single Molecule Force Spectroscopy, Joshua Webster-Ford

Undergraduate Honors Theses

Huntington’s Disease is characterized by an extended (CAG)n repeat found in the huntingtin (Htt) gene. While the typical human contains 6-20 CAG repeats, people with over 36 repeats manifest symptoms of the disease. The extended CAG repeats allow the Htt pre-mRNA transcript to form a hairpin loop structure which can sequester certain vital proteins such as Muscleblind like protein 1 (MBNL1), hindering their functional role. Further understanding the structure of the MBNL1-RNA complex is possible through force spectroscopy, or “optical tweezers”, applied on the single molecule level. Force spectroscopy generates force versus distance plots for individual RNA molecules, revealing the …

Development Of Bifunctional Peptides As Scaffolds For Bifunctional Catalysis And A Novel Method Of Peptide Stapling Using Squaric Esters, Adam X. Wayment

Theses and Dissertations

Enzymes are some of nature's most powerful tools in chemical processes. However, their molecular complexity makes them difficult to synthesize and complicates their application in traditional organic synthesis. Peptides, a building block of enzymes, can be rapidly synthesized and have been used as a possible alternative in achieving enzyme-like catalysis. However, most peptide-based catalysts are limited in reaction-scope and are unable to incorporate traditional organic catalysts. We have designed a helical peptide scaffold capable of being functionalized with a wide variety of organocatalysts as well as transition-metal based catalysts. In order to understand how the peptide structure effects reactivity and …

Transgenic Beetle Modification Via Nanoinjection, Abigail Wallace, Abraham B. Alton, Jane Lundgren, Marianna Ransom, Michelle Teh, Kai Jeffery, Norman Rasmussen, Alex Rolfson, Brian D. Jensen, Colin Dale, Seth Taylor, Sandra Hope

Student Works

The bacterium Sodalis praecaptivus can be transformed with a transgene for expression in the insect. Symbiotic bacteria in the insect’s reproductive tract will be passed on to their offspring. Red fluorescent protein is used for nanoinjection studies.