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Morphometric Analysis Of Enteric Lesions In C3h/Hen Mice Inoculated With Serpulina Hyodysenteriae Serotypes 2 And 4 With Or Without Oral Streptomycin Pretreatment, Jagannatha V. Mysore, Gerald Duhamel

School of Veterinary and Biomedical Sciences: Faculty Publications

The segmental distribution and sequential progression and the role of the indigenous bacterial flora in the development of enteric lesions associated with Serpulina hyodysenteriae infection in laboratory mice have not been defined. We examined the distribution and sequential morphometric changes in the large intestine of mice orally inoculated with S. hyodysenteriae serotypes 2 and 4. To determine the role of colonization resistance conferred by the indigenous bacterial flora, 40 female C3H/HeN mice were administered water alone or water containing 5 mg/mL streptomycin sulfate ad libitum for seven days prior to orogastric inoculation either with S. hyodysenteriae or sterile trypticase soy …

Rapid Assessment Of Drug Susceptibilities Of Mycobacterium Tuberculosis By Means Of Luciferase Reporter Phages, William R. Jacobs Jr., Raul G. Barletta, Rupa Udani, John Chan, Gary Kalkut, Gabriel Sosne, Tobias Kieser, Gary J. Sarkis, Graham F. Hatfull, Barry R. Bloom

School of Veterinary and Biomedical Sciences: Faculty Publications

Effective chemotherapy of tuberculosis requires rapid assessment of drug sensitivity because of the emergence of multidrug-resistant Mycobacterium tuberculosis. Drug susceptibility was assessed by a simple method based on the efficient production of photons by viable mycobacteria infected with specific reporter phages expressing the firefly luciferase gene. Light production was dependent on phage infection, expression of the luciferase gene, and the level of cellular adenosine triphosphate. Signals could be detected within minutes after infection of virulent M. tuberculosis with reporter phages. Culture of conventional strains with antituberculosis drugs, including isoniazid or rifampicin, resulted in extinction of light production. In contrast, …

Do Farmers Really Want To Eradicate Sheep Lice?, Chris Hawkins

Journal of the Department of Agriculture, Western Australia, Series 4

With the termination of Western Australia's Sheep Lice Eradication Campaign in 1993 farmers' views about the eradication of sheep lice are of greater relevance now than in the past. Lice control now rests with individual farmers and continuing local lice cell groups .

A recent survey of producers in the Moora Shire provides some key insights into farmers' thoughts about sheep lice and their eradication.

Genetic Identificationo F A Hantavirusa Ssociated With An Outbreako F Acute Respiratoryi Llness, Stuart T. Nichol, Christina F. Spiropoulou, Sergey Morzunov, Pierre E. Rollin, Thomas G. Ksiazek, Heinz Feldmann, Anthony Sanchez, James E. Childs, Sherif Zaki, Clarence J. Peters

Zoonotics and Wildlife Disease: Publications

A mysterious respiratory illness with high mortality was recently reported in the southwestern United States. Serologic studies implicated the hantaviruses, rodent-borne RNA viruses usually associated elsewhere in the world with hemorrhagic fever with renal syndrome. A genetic detection assay amplified hantavirus-specific DNA fragments from RNA extracted from the tissues of patients and deer mice (Peromyscus maniculatus) caught at or near patient residences. Nucleotide sequence analysis revealed the associated virus to be a new hantavirus and provided a direct genetic link between infection in patients and rodents.

Infection With A Ratborne Hantavirus In Us Residents Is Consistently Associated With Hypertensive Renal Disease, Gregory E. Glass, Alan J. Watson, James W. Leduc, Gabor D. Kelen, Thomas C. Quinn, James E. Childs

Zoonotics and Wildlife Disease: Publications

A survey of 8080 subjects was conducted in Baltimore, examining the association between infection with hantaviruses and renal disease. Two groups (N = 6060) with no known risk factors were selected to establish a baseline antibody prevalence. Overall, antibody prevalence was 0.25%. Seroprevalence increased with age, without sex- or race-related differences. Patients with proteinuria showed the same patterns of infection but were more commonly seropositive (1.46%) than the reference group( OR, 3.23; P < .05). Infection among dialysis patients with end-stage renal disease was 2.76%, significantly higher than in the reference group( OR, 5.03; P < .05). In the proteinuria and the dialysis groups, hantavirus infection was consistently associated with a diagnosis of hypertensive renal disease. The association was unrelated to other chronic renal disease diagnoses. Overall, 6.5% of patients with end-stage renal disease due to hypertension were seropositive for a hantavirus. These data suggest that hantavirus infection is associated with hypertensive renal disease.

Progress Toward Eradication Of Virulent Footrot, Bob Mitchell

Journal of the Department of Agriculture, Western Australia, Series 4

Western Australia is well placed to eradicate virulent footrot from the State's sheep and goat populations.

Between 1950 and 1993 the proportion of sheep flocks with footrot fell from about 15 per cent to less than 2 percent, and the 1990-92 outbreak has been turned around. One hundred and eighty properties (]. 7 per cent) of flocks, mostly in the high rainfall South-West, are in quarantine for footrot today.

This high level of footrot control is the result of strong support from the sheep industry and years of good cooperation between farmers and the Department of Agriculture.

Western Australia leads …

Choosing Shower Dips For Sheep Lice, Tony Higgs, Bob Love

Journal of the Department of Agriculture, Western Australia, Series 4

The performance of all shower dip chemicals for sheep lice can vary depending on the active chemical and the conditions under which they are used.

Department of Agriculture trials have indicated that the wettable powders coumaphos and magnesium fluorosilicate were less effective at eradicating sheep lice than were synthetic pyrethroid and organophosphate dipping chemicals.

However, failure to eradicate sheep lice may be the result of several factors, many of them related to management.

Detection Of Serum Antibody Responses In Cattle With Natural Or Experimental Neospora Infections, Patricia Conrad, Karen Sverlow, Mark Anderson, Joan Rowe, Robert Bondurant, Gwen Tuter, Richard Breitmeyer, California Department Of Food And Agriculture, Animal Health Branch, Mark Thurmond, Alex Ardans, J. P. Dubey, Gerarld Duhamel, Bradd Barr

School of Veterinary and Biomedical Sciences: Faculty Publications

Parasite-specific antibody responses were detected using an indirect fluorescent antibody (IFA) test in cattle that were naturally or experimentally infected with Neospora parasites. The test was developed using Neospora tachyzoites isolated from an aborted bovine fetus and grown in bovine cell cultures (isolate BPA1). In all cases, infections were confirmed by the identification of Neospora tachyzoites and/or bradyzoite cysts in fetal or calf tissues using an immunoperoxidase test procedure. Fifty-five naturally infected cows that aborted Neospora-infected fetuses had titers of 320-5,120 at the time of abortion. The titer of 6 cows that were serologically monitored over a prolonged period decreased …

Reproductive Characteristics Of Rodent Assemblages In Cultivated Regions Of Central Argentina, James N. Mills, Barbara A. Ellis, Kelly T. Mckee, Julio I. Maiztegui, James E. Childs

Zoonotics and Wildlife Disease: Publications

Small mammals were trapped for 2 years at 16 localities on the central-Argentine pampa. Six species (Akodon azarae, Calomys musculinus, C. laucha, Bolomys obscurus, Oligory-zomys flavescens and Mus musculus) accounted for >95% of captures. The major breeding season, as assessed by pregnancies, was September or October through April or May. Mild weather in late autumn and winter of the second season resulted in a relatively longer breeding season during the 2nd year of the study. Females of all six species comprised significantly 50% of captures during the height of the breeding season. For most species, there was …

Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

An alternative approach to structurefunction analysis of vesicular stomatitis virus (VSV) gene products and their interactions with one another during each phase of the viral life cycle is described. We showed previously by using the vaccinia viruslT7 RNA polymerase expression system that when cells expressing the nucleocapsid protein (N), the phosphoprotein (NS), and the large polymerase protein (L) of VSV were superinfected with defective interfering (DI) particles, rapid and efflicient replication and amplification of DI particle RNA occurred. Here, we demonstrate that all five VSV proteins can be expressed simultaneously when cells are cotransfected with plasmids containing the matrix protein …

Habitat Associations And Relative Densities Of Rodent Populations In Cultivated Areas Of Central Argentina, James N. Mills, Barbara A. Ellis, Kelly T. Mckee, Julio I. Maiztegui, James E. Childs

Zoonotics and Wildlife Disease: Publications

Small mammals were livetrapped during 12 months in crop fields and weedy borders at 18 sites in central Argentina. A total of 1,652 mammals of 14 species was captured during3 3,060 trap-nights. Six species of rodents comprised > 95% of captures. Periodically disturbed fields of crops were dominated by Calomys musculinus and C. laucha, and to a lesser extent Mus musculus. A second group composed of Akodon azarae, Bolomys obscures, and Oligoryzomys flavescens primarily inhabited the more stable, weedy borders of cultivated fields. Peaks in relative densities of C. musculinus, C. laucha, and M . musculus …

New Technique Joins The Fight Against Footrot, Laurie Depiazzi, Mike Palmer, David Pitman

Journal of the Department of Agriculture, Western Australia, Series 4

The diagnosis of footrot in sheep and goats is not an easy task. Two main techniques are used for diagnosis - inspection of diseased feet on a farm and laboratory testing of bacteria isolated from foot scrapings. The interpretation of the results obtained by these methods requires a good understanding of the various forms of footrot.

A new laboratory technique has halved the time taken to detect those strains of the bacterium, Bacteroides (Dichelobacter) nodosus, that cause each form of the disease.

Monoclonal Antibodies To The Fusion Protein Of Bovine Respiratory Syncytial Virus, Kent M. Mulkey, Gary A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

Five monoclonal antibodies specific for bovine respiratory syncytial virus were characterized by Western immunoblotting, radioimmunoprecipitation, and epitope mapping assays. The monoclonal antibodies were found to be specific for the fusion protein, and there were at least two antigen binding sites, one of which was neutralizing.

Replication And Amplification Of Defective Interfering Particle Rn As Of Vesicular Stomatitis Virus In Cells Expressing Viral Proteins From Vectors Containing Cloned Cdnas, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

Replication and amplification of RNA genomes of defective interfering (DI) particles of vesicular stomatitis virus (VSV) depend on the expression of viral proteins and have untD now been attained only in ceUs coinfected with helper VSV. In the work described in this report, we used a recombinant vaccinia virus-T7 RNA polymerase expression system to synthesize individual VSV proteins in cells transfected with plasmid DNAs that contain cDNA copies of the VSV genes downstream of the T7 RNA polymerase promoter. In this way, we were able to examine the ability of VSV proteins, individually and in combination, to support DI particle …

Chemical Immobilization Of Red Foxes (Vulpes Vulpes), Terry J. Kreeger, Ulysses S. Seal, John R. Tester

Zoonotics and Wildlife Disease: Publications

Red foxes (Vulpes vulpes) were immobilized with one of the following drug combinations: ketamine/xylazine (n = 22), ketamine/ promazine (n = 35), ketamine/midazolam (n = 13), or tiletamine/zolazepam (n = 22). Foxes given ketamine/xylazine had the shortest induction and longest recovery times relative to other drug combinations, whereas foxes given ketamine/midazolam had the longest induction times. Recommended doses for the various combinations are given. Foxes given ketamine/xylazine were given either 0.1, 0.2, 0.4 mg/kg yohimbine, or saline 40 mm after anesthetic induction. Administration of yohimbine significantly shortened arousal and recovery times relative to control values (P < 0.001).

Determining Matrilines By Antibody Response To Exotic Antigens, Gregory E. Glass, James E. Childs, James W. Leduc, Sandra D. Cassard, Albert D. Donnenberg

Zoonotics and Wildlife Disease: Publications

The antibody responses of female Microtuspennsylvanicus inoculated with a series of antigens not normally encountered under field conditions were examined, and the kinetics of maternally acquired antibody loss in their offspring were determined. The initial antibody response in adults was rapid, peaking in 4-9 weeks, and long-lasting, with a half life of 4-5 months. Antibody levels in females were unaffected by parity, and more than one antigen could be given without affecting circulating-antibody titers. Antibody titers could be enhanced with additional inoculations. Maternal antibody in offspring increased until weaning at 3 weeks then declined exponentially. Minimal detectable titers were reached …

The Live Sheep Export Industry, Richard Norris, Barry Richards, Tony Higgs

Journal of the Department of Agriculture, Western Australia, Series 4

Australia's live sheep trade to Middle Eastern countries is an important export industry. However, about 2 per cent of the sheep die, mainly during the sea voyage. The Western Australian Department of Agriculture has conducted a major research programme to improve the health and welfare of sheep during live export. The programme has'examined many aspects of the export sheep industry, and a substantial part of it has focused on why sheep die during sea transport.

Efficacy Of A Cell Extract From Actinobacillus (Haemophilus) Pleuropneumoniae Serotype 1 Against Disease In Swine, P. J. Fedorka-Cray, M.J. Huether, D.L. Stine, G. A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

We partially characterized a cell extract (CE) from Actinobacillus pleuropneumoniae serotype 1 and used the CE to test the efficacy of secreted proteins against disease. Secreted products from 4-h culture supernatants were precipitated with 20% polyethylene glycol. Analysis of the CE indicated the presence of protein, endotoxin, and carbohydrate. Hemolytic activity to bovine erythrocytes and cytotoxic activity to porcine mononuclear leukocytes was also demonstrated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the CE from a 4-h culture showed a major band at 110 kilodaltons (kDa), while a CE of a 26-h culture indicated the presence of a number of additional …

Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon

School of Veterinary and Biomedical Sciences: Faculty Publications

An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 ,ug of spectinomycin per ml (TSA-S400), TSA-S400 with 25 ,ug each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers …

Late Fall Transmission Of Nematodirus Battus (Nematoda: Trichostrongyloidea) In Western Oregon, Lora G. Rickard, Eric P. Hoberg, Gary L. Zimmerman, Jannell K. Erno

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Studies on Nematodirus battusCrofton and Thomas, 1951, have shown a marked seasonality in the pattern of transmission. The life cycle normally involves only one parasitic generation per year. The infective third-stage larvae develop over the summer within eggs deposited on pasture. Eggs are sensitized by low temperatures during fall and winter; larval hatching follows in the presence of adequate moisture and proper osmotic conditions as the temperature rises above 10°C. This set of conditions, conducive to hatching, is regularly present only during spring; thus, hatching of eggs is concentrated into a few weeks during this time. Outbreaks of clinical …

Natural And Experimental Infection With An Attaching And Effacing Strain Of Escherichia Coli In Calvest, Rodney A. Moxley, David H. Francis

School of Veterinary and Biomedical Sciences: Faculty Publications

Gnotobiotic calves were inoculated with an 05:K4:H^-, urease-positive strain of Escherichia coli isolated from a 2-day-old calf with diarrhea. The calves developed elevated temperatures and passed loose mucoid feces, with or without blood. The E. coli strain was negative for heat-stable and heat-labile enterotoxins but produced high levels of Shiga-like toxin. Bacteria attached diffusely to the epithelium of the large intestine and multifocally to the epithelium of the ileum. The duodenum and jejunum were not affected. At the sites of bacterial attachment, microvilli were effaced, enterocytes were degenerate, and necrosis and exfoliation had occurred. These results confirm a …

The Incidence Of Cryptosporidial Infections In Nebraska Dairy Calves, Douglas Lee Varner

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Within the last decade a new etiologic agent has been identified as a possible cause of diarrhea in humans and domestic animals—that being the protozoan parasite Cryptosporidium. The purpose of this study was to determine the incidence and distribution of Cryptosporidium in the state of Nebraska, to determine if Cryptosporidium occurs more frequently in association with other enteropathogens and to determine about the relation of scouring in the calves to infection with Cryptosporidium. A total of 620 fecal samples from 334 dairy calves from 71 herds in Nebraska were examined for cyptosporidial oocysts using the Sheather’s sugar flotation …

Atrophic Rhinitis In Pigs, A R. Mercy

Journal of the Department of Agriculture, Western Australia, Series 4

Severe atrophic rhinitis in pigs recurred recently in Australia after an apparent absence of some 20 years. The first of these recent cases occurred in a Westerm Australian herd in late 1984 and since then six other local herds have reported the disease. Severe atrophic rhinitis has also been seen recently in South Australia.

Atrophic rhinitis is a complex disease which causes degeneration of the nasal cavity, twisting of the snout and sometimes occasional bleeding from the nose.

It occurs only in pigs and severely affected animals may have a slower growth rate.

Bulletin No 4101 - Flystrike - A Manual For Its Prevention And Control, N Monzu Dr

Bulletins 4000 -

The sheep blowfly is still regarded as the Australian sheep industry's most­damaging pest. Estimates of the cost to the industry, through production losses and prevention and control measures, exceed $ I 00 million in a year. This bulletin comprises the most relevant information available on:

• Blowfly types and life cycles

• Conditions predisposing sheep to flystrike

• The mechanism of flystrike

• Methods of preventing and controlling strike.

• Current research - including the development of a forecasting system for body strike.

Formation Of Influenza Virus Particles Lacking Hemagglutinin On The Viral Envelope, Asit K. Pattnaik, Donald J. Brown, Debi P. Nayak

School of Veterinary and Biomedical Sciences: Faculty Publications

We investigated the intraceUular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (Is) mutants (1s134 and 1s61S) of inOuenza virus AlWSN/33. We found that at the nonpermissive temperature (39.5°C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi complex was blocked and that no additional block was apparent in either the exit from the Golgi complex or post-Golgi complex transport. When MDBK ceUs were infected with these mutant viruses, they produced noninfectious virus particles at 39.5°C. The efficiency of particle formation at 39.5°C was essentiaUy …

Dispelling Some Myths About 'Dermo', J R. Edwards

Journal of the Department of Agriculture, Western Australia, Series 4

There are a lot of misconceptions and myths about dermatophilosis, a disease which can kill young lambs, affect wool quality, make shearing difficult and make sheep susceptible to fly strike.

One misconception is perpetuated by the incorrect use of the name mycotic dermatitis, which implies that a fungus causes the disease. This is not so.

The disease is caused by the bacteria Dermatophilus congolensis, which attacks the sheep's skin and eventually forms a lesion or scab in the wool, and so is better called dermatophilosis, 'dermo' or 'lumpy wool'.

Veterinary Epidemiologist at the Albany Regional Office, J.R. Edwards, discusses …

Identification Of Four Complementary Rna Species In Akabane Virus-Infected Cells, Asit K. Pattnaik, G. Abraham

School of Veterinary and Biomedical Sciences: Faculty Publications

The analysis of RNA extracted from purified Akabane virus demonstrated the presence of three size classes of single-stranded RNAs with sedimentation coefficients of 31S (large, L), 26S (medium, M), and 13S (small, S). Molecular weights of these RNA species were estimated to be 2.15 x 10⁶,1.5 X 10⁶, and 0.48 x 10⁶ for the L, M, and S RNAs, respectively. Hybridization analysis involving viral genomic RNA and RNA from virus-infected cells resulted in the identification of four virus-specific cRNA species in infected cells. These cRNAs were found to be nonpolyadenylated by their inability to bind …

Escherichia Coli Strains Producing Streptococcus Mutans Proteins Responsible For Colonization And Virulence, Roy Curtiss Iii, Robert G. Holt, Raul G. Barletta, James P. Robeson, Shigeno Saito

School of Veterinary and Biomedical Sciences: Faculty Publications

Streptococcus mutans is a principal etiologic agent of dental caries and is likely one of the most ubiquitous bacterial infectious disease agents worldwide. The ability of S. mutans to colonize the oral cavity is due to sucroseindependent and sucrose-dependent adherence to the pellicle-coated tooth surface with glucan facilitated aggregation between cells to result in plaque. Cariogenicity is then caused by the ability of S. mutans in plaque to metabolize free sugars and both extra and intracellular complex carbohydrates to yield predominantly lactic acid. The S. mutans gene products that contribute to colonizing ability and thus virulence include glucosyltransferases, glucanbinding proteins, …

Prostaglandin F2a Induced Nest Building Behavior In The Non-Pregnant Sow, And Some Welfare Considerations, Judith K. Blackshaw

Agribusiness Collection

Nest building behavior, induced with intramuscular injections of prostaglandin F2_a (PGF2_a), was studied in non-pregnant sows. Acute effects, which included salivation, scratching, vomiting, defaecation and ataxia, were also recorded. Sows (Large White x Landrace) were housed in two different environments; six sows in bare pens and six sows in pens provided with bedding material. In all cases except one (bare pen) nest building sequences of differing intensities were recorded. Welfare suggestions include questioning the justification of using a drug (PGF2_a) in pig husbandry, which has unpleasant acute effects, and the suggestion that the provision of …

Infectious Pancreatic Necrosis (Ipn) Of Salmonid Fishes, Phillip E. Mcallister

United States Fish and Wildlife Service: Publications

Infectious pancreatic necrosis (IPN) is a viral disease principally associated with salmonids, although IPN and lPN-like viruses have been isolated from various nonsa1rnonid fish and marine invertebrates. Acute infection occurs in 1- to 4-month-old salmonids and can result in cumulative mortality approaching 100%. In contrast, fish 6 months old or older can undergo subclinical or inapparent infection, but experience no significant mortality. Survivors of the disease can become lifelong carriers of the virus. Epizootics of IPN have been reported worldwide, and several virus serotypes are recognized. The disease known as IPN was probably first described by M'Gonigle (1941) as acute …