Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Veterinary Medicine

Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 1831 - 1860 of 2153

Full-Text Articles in Entire DC Network

Michigan Bovine Tuberculosis Eradication Project: 2004 Activities Report And Conference Proceedings Jun 2004

Michigan Bovine Tuberculosis Eradication Project: 2004 Activities Report And Conference Proceedings

Michigan Bovine Tuberculosis Bibliography and Database

On June 9 and 10, 2004, the State of Michigan and U.S. Department of Agriculture hosted the eighth annual Bovine Tuberculosis (TB) Conference, with guest speakers from Georgia, Colorado, Iowa, New York and two Canadian provinces. Our guests came together from across North America to receive policy updates and share the latest scientific data and innovative research on bovine TB.

Among other things, the speakers shared insight on: the promotion of biosecurity; the $30 million annual costs of TB eradication in New Zealand; the latest research in new and improved blood tests; and the first season results of the Michigan …

Go to article

Rabies In Newfoundland & Labrador May 2004

Rabies In Newfoundland & Labrador

Zoonotics and Wildlife Disease: Publications

Rabies is a deadly viral disease of the brain spread by the bite of infected mammals. It is most commonly seen in red or arctic foxes in this province though other wild animals such as bats may carry the disease. Sick foxes can bite other foxes, dogs, cats, wolves, caribou, other animals and people and make them sick. Once bitten by an animal with rabies it can take from two weeks to six months before the animal shows signs of the disease. Though uncommon, this period can be as short as 4 days or as long as a year or …

Go to article

Animal Health Matters, David H. Zeman Apr 2004

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1-Equipment grant to support bioterrorism preparedness and TSE testing at the ADRDL [Page] 1- Call for Cases for the Summer Meeting Diagnostic News [Page] 2- BVD ear notch test – submission guidelines [Page] 2- Tritrichomonas pouch culture procedures at the ADRDL [Page] 2- Equine infectious anemia testing Research News [Page] 3- VSD and the Governor’s 2010 Research Initiative Extension News [Page] 3-Toxoplasma gondii and schizophrenia? [Page] 4- A survey of agents associated with neonatal diarrhea in Iowa swine including C. difficile and PRRSV [Page] 4- Avian influenza: should we be concerned about poultry in Asia?

Go to article

“French Heartworm” Infection Of Dogs & Foxes In Newfoundland Apr 2004

“French Heartworm” Infection Of Dogs & Foxes In Newfoundland

Zoonotics and Wildlife Disease: Publications

French Heartworm is the common name for a parasitic disease affecting foxes, dogs, and other members of the dog family. Caused by the nematode (worm) Angiostrongylus vasorum, this disease was first thought to exist in France in the mid 1800's. It is now considered to exist generally in Europe but has not been reported in North America other than on the Island of Newfoundland, with the exception of infected animals imported from Europe but diagnosed in North America. The first case recorded in Newfoundland was in Aquaforte (Avalon Peninsula) in 1973.
When and how it might have come over …

Go to article

Seroprevalence Of Bartonella Infection In American Free-Ranging And Captive Pumas (Felis Concolor) And Bobcats (Lynx Rufus), Bruno B. Chomel, Yoko Kikuchi, Janice S. Martenson, Melodie E. Roelke-Parker, Chao-Chin Chang, Rickie W. Kasten, Janet E. Foley, John Laudre, Kerry Murphy, Pamela K. Swift, Vicki L. Kramer, Stephen J. O'Brien Apr 2004

Seroprevalence Of Bartonella Infection In American Free-Ranging And Captive Pumas (Felis Concolor) And Bobcats (Lynx Rufus), Bruno B. Chomel, Yoko Kikuchi, Janice S. Martenson, Melodie E. Roelke-Parker, Chao-Chin Chang, Rickie W. Kasten, Janet E. Foley, John Laudre, Kerry Murphy, Pamela K. Swift, Vicki L. Kramer, Stephen J. O'Brien

Zoonotics and Wildlife Disease: Publications

Bartonella henselae is the main agent of cat scratch disease in humans and domestic cats are the main reservoir of this bacterium. We conducted a serosurvey to investigate the role of American wild felids as a potential reservoir of Bartonella species. A total of 479 samples (439 serum samples and 40 Nobuto strips) collected between 1984 and 1999 from pumas (Felis concolor) and 91 samples (58 serum samples and 33 Nobuto strips) collected from bobcats (Lynx rufus) in North America, Central America and South America were screened for B. henselae antibodies. The overall prevalence of B. …

Go to article

Chronic Wasting Disease—Prion Disease In The Wild, Steve Bunk Apr 2004

Chronic Wasting Disease—Prion Disease In The Wild, Steve Bunk

Zoonotics and Wildlife Disease: Publications

In 1967, mule deer in a research facility near Fort Collins, Colorado, in the United States apparently began to react badly to their captivity. At least, that was the guess of researchers working on the natural history and nutrition of the deer, which became listless and showed signs of depressed mood, hanging their heads and lowering their ears. They lost appetite and weight. Then they died—of emaciation, pneumonia, and other complications—or were euthanized. The scientists dubbed it chronic wasting disease (CWD), and for years they thought it might be caused by stress, nutritional deficiencies, or poisoning. A decade later, CWD …

Go to article

Genomically Intact Endogenous Feline Leukemia Viruses Of Recent Origin, Alfred L. Roca, Jill Pecon-Slattery, Stephen J. O'Brien Apr 2004

Genomically Intact Endogenous Feline Leukemia Viruses Of Recent Origin, Alfred L. Roca, Jill Pecon-Slattery, Stephen J. O'Brien

Biology Faculty Articles

We isolated and sequenced two complete endogenous feline leukemia viruses (enFeLVs), designated enFeLV-AGTT and enFeLV-GGAG. In enFeLV-AGTT, the open reading frames are reminiscent of a functioning FeLV genome, and the 5′ and 3′ long terminal repeat sequences are identical. Neither endogenous provirus is genetically fixed in cats but polymorphic, with 8.9 and 15.2% prevalence for enFeLV-AGTT and enFeLV-GGAG, respectively, among a survey of domestic cats. Neither provirus was found in the genomes of related species of the Felis genus, previously shown to harbor enFeLVs. The absence of mutational divergence, polymorphic incidence in cats, and absence in related species suggest that …

Go to article

Tuberculosis, John B. Kaneene, Charles O. Thoen Mar 2004

Tuberculosis, John B. Kaneene, Charles O. Thoen

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis is a term that encompasses various diseases caused by bacteria of the Mycobacterium tuberculosis complex, including M tuberculosis, M bovis, M africanum, and other mycobacterial species. Whereas M tuberculosis infection is largely spread from human to human, M bovis infection has been identified as a zoonotic disease with most cases of human infection attributable to animal sources. The mycobacteria other than tuberculosis complex (MOTT), which includes M avium subsp avium and M avium subsp intracellulare isolated from animals, has been isolated from immune-compromised humans (ie, those with human immunodeficiency virus [HIV] infection), but seldom from immunocompetent humans. Recently, …

Go to article

Chronic Wasting Disease Management In Nebraska Jan 2004

Chronic Wasting Disease Management In Nebraska

Zoonotics and Wildlife Disease: Publications

The presence of Chronic Wasting Disease (CWD) in free-roaming and domesticated cervine animal populations of deer and elk in the state of Nebraska presents a threat to the natural resources of the state. The presence of this disease could drastically restrict the successful achievement of the goals and objectives of the Nebraska Game and Parks Commission (NGPC) as set forth in their long range plan “Focusing on the Future…A Plan for Nebraska’s Fish, Wildlife and Parkland Resources”. Objective 3 in the Big Game section of this plan is to “Monitor the status and health of deer, so reasonable assumptions may …

Go to article

Decreased Shedding Of Escherichia Coli O157:H7 By Cattle Following Vaccination With Type Iii Secreted Proteins, Andrew A. Potter, Sandra Klashinsky, Yuling Li, Elizabeth Frey, Hugh Townsend, Dragan Rogan, Galen E. Erickson, Susanne Hinkley, Terry J. Klopfenstein, Rodney A. Moxley, David R. Smith, B. Brett Finlay Jan 2004

Decreased Shedding Of Escherichia Coli O157:H7 By Cattle Following Vaccination With Type Iii Secreted Proteins, Andrew A. Potter, Sandra Klashinsky, Yuling Li, Elizabeth Frey, Hugh Townsend, Dragan Rogan, Galen E. Erickson, Susanne Hinkley, Terry J. Klopfenstein, Rodney A. Moxley, David R. Smith, B. Brett Finlay

School of Veterinary and Biomedical Sciences: Faculty Publications

Cattle are an important reservoir of Escherichia coli O157:H7 leading to contamination of food and water, and subsequent human disease. This pathogen colonizes its hosts by producing several proteins such as Tir and EspA that are secreted by a type III secretion system. These proteins play a role in colonization of the intestine, suggesting that they might be useful targets for the development of a vaccine to reduce levels of this organism in cattle. Vaccination of cattle with proteins secreted by E. coli O157:H7 significantly reduced the numbers of bacteria shed in feces, the numbers of animals that shed, and …

Go to article

Estimating The True Prevalence Of Mycobacterium Bovis In Hunter-Harvested White-Tailed Deer In Michigan, Daniel J. O’Brien, Stephen M. Schmitt, Dale E. Berry, Scott D. Fitzgerald, Jolene R. Vanneste, Timothy J. Lyon, Diane Magsig, Jean S. Fierke, Thomas M. Cooley, Laura S. Zwick, Bruce V. Thomsen Jan 2004

Estimating The True Prevalence Of Mycobacterium Bovis In Hunter-Harvested White-Tailed Deer In Michigan, Daniel J. O’Brien, Stephen M. Schmitt, Dale E. Berry, Scott D. Fitzgerald, Jolene R. Vanneste, Timothy J. Lyon, Diane Magsig, Jean S. Fierke, Thomas M. Cooley, Laura S. Zwick, Bruce V. Thomsen

Michigan Bovine Tuberculosis Bibliography and Database

Apparent prevalence, although useful as a consistent index, may underestimate the true prevalence of disease. In Michigan, the ability to estimate the true prevalence of bovine tuberculosis (TB; caused by Mycobacterium bovis) in free-ranging white-tailed deer (Odocoileus virginianus) will become increasingly important to accurately assess progress towards eradication. Our objectives were threefold: to estimate the true prevalence of M. bovis in free-ranging deer in Michigan, to evaluate the effectiveness of existing TB surveillance methods, and to indirectly assess whether TB epidemiologic data from captive cervid herds can be meaningfully extrapolated to free-ranging populations. The study population consisted …

Go to article

A Processed Pseudogene Contributes To Apparent Mule Deer Prion Gene Heterogeneity, Kelly A. Brayton, Katherine I. O'Rourke, Amy K. Lyda, Michael W. Miller, Donald P. Knowles Jr. Jan 2004

A Processed Pseudogene Contributes To Apparent Mule Deer Prion Gene Heterogeneity, Kelly A. Brayton, Katherine I. O'Rourke, Amy K. Lyda, Michael W. Miller, Donald P. Knowles Jr.

Zoonotics and Wildlife Disease: Publications

Pathogenesis and transmission of the prion disorders (transmissible spongiform encephalopathies, TSEs) are mediated by a modified isoform of the prion protein (PrP). Prion protein gene (PRNP) alleles associated with relative susceptibility to TSE have been identified in sheep, humans and possibly elk. Comparable data have not been derived for mule deer, a species susceptible to the TSE chronic wasting disease (CWD). Initial analysis of the open reading frame (ORF) in exon 3 of the mule deer PRNP gene revealed polymorphisms in all 145 samples analyzed, with 10 potential polymorphic sites. Because 144/145 (99.3%) of the samples were heterozygous …

Go to article

Resistance To Scrapie In Prp Arr/Arq Heterozygous Sheep Is Not Caused By Preferential Allelic Use, P. A. Caplazi, Katherine I. O'Rourke, T. V. Baszler Jan 2004

Resistance To Scrapie In Prp Arr/Arq Heterozygous Sheep Is Not Caused By Preferential Allelic Use, P. A. Caplazi, Katherine I. O'Rourke, T. V. Baszler

Zoonotics and Wildlife Disease: Publications

Scrapie is a prion disease in sheep that, similar to Kuru or variant Creutzfeldt-Jakob disease (vCJD), requires both prion exposure and a genetic susceptibility. Susceptibility is defined by amino acid polymorphisms in the single copy prion protein (PrP) gene, Prnp. Specifically, in sheep homozygous for A at position 136 and R at position 154, susceptibility is defined by R or Q at position 171. The genotype ARQ/ARQ confers susceptibility, whereas (apart from a few reported exceptions) genotypes ARR/ARQ and ARR/ARR confer resistance. Similarly, a M/V polymorphism at Prnp codon 129 determines susceptibility to vCJD and Kuru,4 with partial resistance conferred …

Go to article

Polymorphisms In The Prion Precursor Functional Gene But Not The Pseudogene Are Associated With Susceptibility To Chronic Wasting Disease In White-Tailed Deer, Katherine I. O'Rourke, Terry R. Spraker, Linda K. Hamburg, Thomas E. Besser, Kelly A. Brayton, Donald P. Knowles Jan 2004

Polymorphisms In The Prion Precursor Functional Gene But Not The Pseudogene Are Associated With Susceptibility To Chronic Wasting Disease In White-Tailed Deer, Katherine I. O'Rourke, Terry R. Spraker, Linda K. Hamburg, Thomas E. Besser, Kelly A. Brayton, Donald P. Knowles

Zoonotics and Wildlife Disease: Publications

Chronic wasting disease (CWD) status and PrP genotypes were determined for a group of 133 wild white-tailed deer in a 780 acre enclosure in western Nebraska, USA. Approximately half of the deer tested showed evidence of PrPd in the brainstem or lymphoid tissues. Four PRNP alleles encoding amino acid substitutions were identified, with substitutions at residues 95 (Q→H), 96 (G→S) or 116 (A→G), each with serine (S) at residue 138. In addition, a processed pseudogene with two alleles encoding five or six copies of the octapeptide repeat was identified in 26% of the deer. Both alleles encoded asparagine (N) …

Go to article

Transmission Of Transmissible Mink Encephalopathy To Raccoons (Procyon Lotor) By Intracerebral Inoculation, Amir N. Hamir, Janice M. Miller, Katherine I. O'Rourke, Jason C. Bartz, Mick J. Stack, Melanie J. Chaplin Jan 2004

Transmission Of Transmissible Mink Encephalopathy To Raccoons (Procyon Lotor) By Intracerebral Inoculation, Amir N. Hamir, Janice M. Miller, Katherine I. O'Rourke, Jason C. Bartz, Mick J. Stack, Melanie J. Chaplin

Zoonotics and Wildlife Disease: Publications

To determine the transmissibility of transmissible mink encephalopathy (TME) agent to raccoons and to provide information about clinical course, lesions, and suitability of currently used diagnostic procedures for detection of transmissible spongiform encephalopathies (TSEs) in raccoons, 4 raccoon kits were inoculated intracerebrally with a brain suspension from mink experimentally infected with TME. One uninoculated raccoon kit served as a control. All 4 animals in the TME-inoculated group showed clinical signs of neurologic disorder and were euthanized between 21 and 23 weeks postinoculation (PI). Necropsy examinations revealed no gross lesions. Spongiform encephalopathy was observed by light microscopy, and the presence of …

Go to article

Variable Patterns Of Distribution Of Prpcwd In The Obex And Cranial Lymphoid Tissues Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Subclinical Chronic Wasting Disease, T. R. Spraker, A. Balachandran, D. Zhuang, Katherine I. O'Rourke Jan 2004

Variable Patterns Of Distribution Of Prpcwd In The Obex And Cranial Lymphoid Tissues Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Subclinical Chronic Wasting Disease, T. R. Spraker, A. Balachandran, D. Zhuang, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Sections of medulla oblongata. taken at the level of the obex, palatine tonsil and medial retropharyngeal lymph node from 10,269 captive Rocky Mountain elk (Cervus elaphus nelsoni), were examined by immunohistochemical staining with monoclonal antibody for the prion protein associated with the transmissible spongiform encephalopathy of cervids, chronic wasting disease (PrPCWD). The protein was detected in 226 of them. On the basis of the anatomical location of the deposits in the brainstem of 183 elk, four distinct patterns of distribution of PrPCWD within the parasympathetic region of the dorsal motor nucleus of the vagus nerve …

Go to article

Biology Of PrpSc Accumulation In Two Natural Scrapie-Infected Sheep Flocks, Patrick Caplazi, Katherine I. O'Rourke, Cynthia Wolf, Daniel Shaw, Timothy V. Baszler Jan 2004

Biology Of PrpSc Accumulation In Two Natural Scrapie-Infected Sheep Flocks, Patrick Caplazi, Katherine I. O'Rourke, Cynthia Wolf, Daniel Shaw, Timothy V. Baszler

Zoonotics and Wildlife Disease: Publications

Sheep scrapie is a prion disease that requires interaction of exogenous prions with host prion protein (PrP) supporting prion formation. Disease is associated with deposition of a host-generated conformational variant of PrP, PrPsc, in a variety of tissues, including brain, resulting in fatal spongiform encephalopathy. Efficiency of PrPsc formation is determined by polymorphisms in the PrP-coding sequence. This article adds to previous data of natural sheep scrapie, concentrating on the effect of host genotype and age on PrPsc accumulation patterns during preclinical and clinical disease. Two entire scrapie-infected, predominantly Suffolk-cross, sheep flocks euthanized for regulatory purposes …

Go to article

Bovine Tuberculosis In Swedish Farmed Deer: Detection And Control Of The Disease, Helene Wahlström Jan 2004

Bovine Tuberculosis In Swedish Farmed Deer: Detection And Control Of The Disease, Helene Wahlström

Michigan Bovine Tuberculosis Bibliography and Database

Bovine tuberculosis (BTB) was introduced into Swedish farmed deer herds in 1987. Epidemiological investigations showed that 10 deer herds had become infected (July 1994) and a common source of infection, a consignment of 168 imported farmed fallow deer, was identified (I).

As trace-back of all imported and in-contact deer was not possible, a control program, based on tuberculin testing, was implemented in July 1994. As Sweden has been free from BTB since 1958, few practicing veterinarians had experience in tuberculin testing. In this test, result relies on the skill, experience and conscientiousness of the testing veterinarian. Deficiencies in performing the …

Go to article

Department Of Veterinary And Biomedical Sciences 2004 Annual Report Jan 2004

Department Of Veterinary And Biomedical Sciences 2004 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Veterinary and Biomedical Sciences Personnel
VBMS Teaching Program
VBMS Research Program
International Activities, 2004
Veterinary Extension Program. 2004
VBMS Grants and Contracts Program. 2004
Refereed Publications by VBMS Faculty in 2004
Department of Veterinary and Biomedical Sciences. Selected Committees, Editorial and Other Appointments. 2004
Articles Regarding the Department in 2004
Departmental Budget Summaries. 2004
Nebraska Agricultural Statistics. 2003/2004

Go to article

Phosphorylation Of Vesicular Stomatitis Virus Phosphoprotein P Is Indispensable For Virus Growth†, Subash C. Das, Asit K. Pattnaik Jan 2004

Phosphorylation Of Vesicular Stomatitis Virus Phosphoprotein P Is Indispensable For Virus Growth†, Subash C. Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P) of vesicular stomatitis virus (VSV) is an essential subunit of the viral RNAdependent RNA polymerase (RdRp) complex. It is phosphorylated at two different domains. Using defective interfering (DI) RNA or minigenomic RNA templates, we previously demonstrated that phosphorylation within the amino-terminal domain I is essential for transcription, whereas phosphorylation within the carboxyterminal domain II is necessary for replication. For the present study, we examined the role of the phosphorylation of residues in these domains in the life cycle of VSV. Various mutant P coding sequences were inserted into a full-length cDNA clone of VSV, and the virus …

Go to article

Can We Use Vitamin E As An Anti-Tumoral Agent For The Treatment Of Tumors In Fish?, Olcay Hi̇sar Jan 2004

Can We Use Vitamin E As An Anti-Tumoral Agent For The Treatment Of Tumors In Fish?, Olcay Hi̇sar

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Go to article

Bovine Tuberculosis (Mycobacterium Bovis) In Wildlife In Spain, Alicia Aranaz, Lucía De Juan, Natalia Montero, Celia Sanchez, Margarita Galka, Consuelo Delso, Julio Alvarez, Beatriz Romero, Javier Bezos, Ana I. Vela, Victor Briones, Ana Mateos, Lucas Domínguez Jan 2004

Bovine Tuberculosis (Mycobacterium Bovis) In Wildlife In Spain, Alicia Aranaz, Lucía De Juan, Natalia Montero, Celia Sanchez, Margarita Galka, Consuelo Delso, Julio Alvarez, Beatriz Romero, Javier Bezos, Ana I. Vela, Victor Briones, Ana Mateos, Lucas Domínguez

Michigan Bovine Tuberculosis Bibliography and Database

Mycobacterium bovis infection in wildlife and feral species is a potential source of infection for livestock and a threat to protected and endangered species. The aim of this study was to identify Spanish wild animal species infected with M. bovis through bacteriological culture and spacer oligonucleotide typing (spoligotyping) of isolates for epidemiological purposes. This study included samples from red deer (Cervus elaphus), fallow deer (Dama dama), wild boar (Sus scrofa), Iberian lynx (Lynx pardina), hare (Lepus europaeus), and cattle (Bos taurus). They were collected in several geographical areas …

Go to article

Ultrastructure Of Mycobacterium Marinum Granuloma In Striped Bass Morone Saxatilis, D. T. Gauthier, W. K. Vogelbein, C. A. Ottinger Jan 2004

Ultrastructure Of Mycobacterium Marinum Granuloma In Striped Bass Morone Saxatilis, D. T. Gauthier, W. K. Vogelbein, C. A. Ottinger

Biological Sciences Faculty Publications

An emerging epizootic of mycobacteriosis currently threatens striped bass Morone saxatilis populations in Chesapeake Bay, USA. Several species of mycobacteria, including Mycobacterium marinum, species resembling M. avium, M. gordonae, M. peregrinum, M. scrofulaceum and M. terrae, and the new species M. shottsii have been isolated from diseased and healthy bass. In this study, we describe the ultrastructure of developing M. marinum granulomas in experimentally infected bass over a period of 45 wk. The primary host response to injected mycobacteria was formation of large macrophage aggregations containing phagocytosed bacilli. M. marinum were always contained within phagosomes. Close …

Go to article

Histamine As An Aid To Biopsy Of Third Eyelid Lymphoid Tissue In Sheep, S. Bender, J. Alverson, L. M. Herrmann, Katherine I. O'Rourke Jan 2004

Histamine As An Aid To Biopsy Of Third Eyelid Lymphoid Tissue In Sheep, S. Bender, J. Alverson, L. M. Herrmann, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

TRANSMISSIBLE spongiform encephalopathies, of which ovine scrapie is a prototype, are neurodegenerative disorders characterised by the accumulation of an abnormal isoform (PrP-scrapie or PrPSc) of a normal cellular protein (PrPC) (Prusiner 1982). Characteristics of the disease include long incubation periods, and a progressive and chronic clinical course resulting in death (Fraser 1976). PrPsc is detectable during the preclinical stages of scrapie in peripheral lymph nodes (Ikegami and others 1991, Muramatsu and others 1993), in lymphoid follicles of the tonsils (Schreuder and others 1996) and in lymphoid follicles of the nictitating membrane or third eyelid (O'Rourke …

Go to article

Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally Jan 2004

Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally

LSU Doctoral Dissertations

The overall goals of this research were to develop a reproducible method of detecting stable DNA insertion into Japanese quail and provide a method for gene location on avian chromosomes. This research resulted in the development of a different method of obtaining chromosome spreads in Japanese quail, the establishment of primed in situ hybridization as a method for the chromosomal gene detection in birds, development of Teflon-coated coverslip slides to facilitate laser microdissection of 0.5 ƒÝm samples, and chromosomal identification of proinsulin transgene insertions by laser microdissection and nucleotide sequence from G2 Japanese quail. The 28S rDNA was found on …

Go to article

Estimation Of The Effect Of Misclassifications On Diagnostic Test Performance In Two Persistent Bovine Viral Infections, Kimberly Orr Jan 2004

Estimation Of The Effect Of Misclassifications On Diagnostic Test Performance In Two Persistent Bovine Viral Infections, Kimberly Orr

LSU Doctoral Dissertations

Validation of diagnostic assay performance is hampered where no gold standard exists. Bayesian estimates of the sensitivity and specificity were applied to studies of two persistent bovine infections: bovine immunodeficiency virus (BIV), and bovine viral diarrhea virus (BVDV). BVDV pathogenesis is well described. Losses of $20 million in 1992 alone led the Danish government to mandate eradication. Two enzyme-linked immunosorbent assay (ELISA) tests were developed; one antibody-based to detect exposure, one antigen-based to detect persistent infection. Bayesian estimates of the sensitivity and specificity were 96.6% and 99.2%, and 98.2% and 99.8% for the antibody and antigen ELISA, respectively. Maximum Likelihood …

Go to article

Herpes Simplex Virus Type 2 Induces Rapid Cell Death And Functional Impairment Of Murine Dendritic Cells In Vitro, Clinton J. Jones, M. Fernandez, K. Herc, L. Bosnjak, M. Miranda-Saksena, R. A. Boadle, A. Cunningham Oct 2003

Herpes Simplex Virus Type 2 Induces Rapid Cell Death And Functional Impairment Of Murine Dendritic Cells In Vitro, Clinton J. Jones, M. Fernandez, K. Herc, L. Bosnjak, M. Miranda-Saksena, R. A. Boadle, A. Cunningham

School of Veterinary and Biomedical Sciences: Faculty Publications

Dendritic cells (DC) are critical for stimulation of naive T cells. Little is known about the effect of herpes simplex virus type 2 (HSV-2) infection on DC structure or function or if the observed effects of HSV-1 on human DC are reproduced in murine DC. Here, we demonstrate that by 12 h postinfection, wild-type (wt) HSV-2 (186) abortively infected murine bone marrow-derived DC and induced early cell death compared to UV-inactivated HSV-2 or mock-infected DC. HSV-2-induced loss of DC viability was more rapid than that induced by HSV-1 and was due, in part, to apoptosis, as shown by TEM, caspase-3 …

Go to article

Sheep Updates 2003 - Plenaries, Ashley Mercy, David Scobie, Neil Buchanan, Kevin Bell, John Milton, Graeme Martin, Bob Hall, Maurie Stephen, Rob Woodgate Aug 2003

Sheep Updates 2003 - Plenaries, Ashley Mercy, David Scobie, Neil Buchanan, Kevin Bell, John Milton, Graeme Martin, Bob Hall, Maurie Stephen, Rob Woodgate

Sheep Updates

This session covers eight papers from different authors:1. Biosecurity in the sheep industry will improve production and profits Ashley Mercy, Department of Agriculture, Western Australia 2. Easy care sheep David Scobie, AgResearch, PO Box 60 Lincoln, 8152, New Zealand 3. Targeting the market’s requirements - live sheep exports Neil Buchanan, Manager Animal Health Operations, Primary Industries & Resources, South Australia 4. Setting flock breeding objectives Kevin Bell, Sheep Management and Production Consultants, Kojonup WA 5. Future feeding of sheep in Western Australia John Milton and Graeme Martin, The University of Western Australia 6. Recipe for getting back into Sheep Bob …

Go to article

The Economics Of Managing Wildlife Disease, Richard D. Horan, Christopher A. Wolf Jul 2003

The Economics Of Managing Wildlife Disease, Richard D. Horan, Christopher A. Wolf

Michigan Bovine Tuberculosis Bibliography and Database

The spread of infectious disease among and between wild and domesticated animals has become a major problem worldwide. Upon analyzing the dynamics of wildlife growth and infection when the diseased animals cannot be identified separately from healthy wildlife prior to the kill, we find that harvest-based strategies alone have no impact on disease transmission. Other controls that directly influence disease transmission and/or mortality are required. Next, we analyze the socially optimal management of infectious wildlife. The model is applied to the problem of bovine tuberculosis among Michigan white-tailed deer, with non-selective harvests and supplemental feeding being the control variables. Using …

Go to article

Review Of Molecular Infection Biology: Interactions Between Mircoorganisms And Cells By Jörg Hacker And Jürgen Heesemann, Jeffrey D. Cirillo Jun 2003

Review Of Molecular Infection Biology: Interactions Between Mircoorganisms And Cells By Jörg Hacker And Jürgen Heesemann, Jeffrey D. Cirillo

School of Veterinary and Biomedical Sciences: Faculty Publications

Pathogenesis is one potential outcome of the constant struggle between host defenses and the desire of microorganisms to acquire a privileged niche. This mutually competitive evolutionary process has resulted in interactions among complex and often elegant systems throughout the course of infections. The complexity of these interactions and the large number of infectious agents that exist makes the introduction of students to infectious diseases, as well as a comprehensive review of the field, an extremely daunting task. This book provides just such a comprehensive and timely description of the field in a manner that is easily understandable and enjoyable to …

Go to article

First Prev Next Last