Digital Commons Network^™

Functional Blockade Of E-Selectin In Tumor-Associated Vessels Enhances Anti-Tumor Effect Of Doxorubicin In Breast Cancer, Yoshihiro Morita, Macall Leslie, Hiroyasu Kameyama, Ganesh L. Lokesh, Norihisa Ichimura, Rachel Davis, Natalie Hills, Nafis Hasan, Roy Zhang, Yuji Kondo, David G. Gorenstein, David E. Volk, Inna Chervoneva, Hallgeir Rui, Takemi Tanaka

Faculty Publications

Chemotherapy is a mainstay of treatment for solid tumors. However, little is known about how therapy-induced immune cell infiltration may affect therapy response. We found substantial CD45 immune cell density adjacent to E-selectin expressing inflamed vessels in doxorubicin (DOX)-treated residual human breast tumors. While CD45 level was significantly elevated in DOX-treated wildtype mice, it remained unchanged in DOX-treated tumors from E-selectin null mice. Similarly, intravenous administration of anti-E-selectin aptamer (ESTA) resulted in a significant reduction in CD45 immune cell density in DOX-treated residual tumors, which coincided with a delay in tumor growth and lung metastasis in MMTV-pyMT mice. Additionally, both …

Role Of Changes In Sars-Cov-2 Spike Protein In The Interaction With The Human Ace2 Receptor: An In Silico Analysis, Joseph Thomas Ortega

Faculty Scholarship

Many human viral diseases are a consequence of a zoonotic event. Some of the diseases caused by these zoono- tic events have affected millions of people around the world, some of which have resulted in high rates of mor- bidity/mortality in humans. Changes in the viral proteins that function as ligands of the host receptor may pro- mote the spillover between species. The most recent of these zoonotic events that have caused an ongoing epi- demic of high magnitude is the Covid-19 epidemics caused by SARS-CoV-2. The aim of this study was to de- termine the mutation(s) in the sequence …

Unrevealing Sequence And Structural Features Of Novel Coronavirus Using In Silico Approaches: The Main Protease As Molecular Target, Joseph Thomas Ortega

Faculty Scholarship

Direct-acting antivirals are effective tools to control viral infections. SARS-CoV-2 is a coronavirus associated with the epidemiological outbreak in late 2019. Previous reports showed that HIV-1 protease inhibitors could block SARS-CoV main protease. Based on that and using an in silico approach, we evaluated SARS-CoV-2 main protease as a target for HIV-1 protease inhibitors to reveal the structural features related to their antiviral effect. Our results showed that several HIV inhibitors such as lopinavir, ritonavir, and saquinavir produce strong interaction with the active site of SARS-CoV-2 main protease. Furthermore, broad library protease inhibitors obtained from PubChem and ZINC (www.zinc.docking.org) were …

2020-03-16 A Message From Chancellor Roth, Paul B. Roth

HSC Messages from the Chancellor

No abstract provided.

Network-Based Drug Repurposing For Novel Coronavirus 2019-Ncov/Sars-Cov-2, Yuan Hou, Feixiong Cheng

Faculty Scholarship

Human coronaviruses (HCoVs), including severe acute respiratory syndrome coronavirus (SARS-CoV) and 2019 novel coronavirus (2019-nCoV, also known as SARS-CoV-2), lead global epidemics with high morbidity and mortality. However, there are currently no effective drugs targeting 2019-nCoV/SARS-CoV-2. Drug repurposing, representing as an effective drug discovery strategy from existing drugs, could shorten the time and reduce the cost compared to de novo drug discovery. In this study, we present an integrative, antiviral drug repurposing methodology implementing a systems pharmacology-based network medicine platform, quantifying the interplay between the HCoV-host interactome and drug targets in the human protein-protein interaction network. Phylogenetic analyses of 15 …

Is Romania Ready To Face The Novel Coronavirus (Covid-19) Outbreak? The Role Of Incoming Travelers And That Of Romanian Diaspora, Iulian Gherghel

Student Scholarship

Letter to editor of Travel Medicine and Infectious Disease

Fate And Biofilm Formation Of Wild-Type And Pressure-Stressed Pathogens Of Public Health Concern In Surface Water And On Abiotic Surfaces, Md Niamul Kabir, Sadiye Aras, Sabrina Wadood, Shahid Chowdhury, Aliyar Cyrus Fouladkhah

Agricultural and Environmental Sciences Faculty Research

Since the historic outbreak near Broad Street in London, which serves as cornerstone of modern epidemiology, infectious diseases spread in surface and sub-surface water has been a persisting public health challenge. The current study investigated persistence of wild-type and pressure-stressed Listeria monocytogenes, Escherichia coli O157:H7, and non-typhoidal Salmonella enterica serovars in surface water stored aerobically for up to 28 days at 5, 25, and 37 °C. Additionally, biofilm formation of wild-type and pressure-stressed non-typhoidal Salmonella serovars were monitored on surface of stainless steel and rubber coupons for 28 days at 25 and 37 °C. While L. monocytogenes exhibited a lower …

Characterization Of Extracellular Vesicles Isolated From Human Milk Using A Precipitation-Based Method, Diana C. Bickmore, John Miklavcic

Food Science Faculty Articles and Research

Extracellular vesicles (EV) function in intercellular communication, and those in human milk may confer immunologic benefits to infants. Methods of EV isolation such as ultracentrifugation (UC) may not be feasible for the study of EVs in human milk due to the need for large sample volume. A technique to isolate EVs from a small volume of human milk using a precipitation reagent is described herein. Electron microscopy, nanoparticle tracking analysis, and semi-quantitative antibody array were conducted to confirm isolation of human milk EVs. Count, size, protein content, and fatty acid quantification of EVs were determined. This isolation technique yielded 8.9 …

Abriendo Futuros: A Program For Rural Indigenous Girls In Yucatan, Mexico, Fabiola Romero, Ludivine Cicolella, Silvana Larrea, A. Fallone, Isabel Vieitez Martínez

Poverty, Gender, and Youth

The Abriendo Futuros (AF) program builds on the Council’s global experience of designing and implementing successful girl-centered programs in Africa, Asia, the Middle East, and Latin America and the Caribbean. AF aims to improve the living conditions of Mayan girls (10–18 years) and contribute to the achievement of gender equity in the Yucatan. The program’s community-focused strategy seeks to empower girls through interpersonal communication and participatory workshops. Girls in selected communities are divided into age groups (10–14 and 15–18) that meet weekly with a mentor in safe spaces. The program’s mentors are young women aged 20–30 from the communities, who …

Louisville Coronavirus Surveillance Program, Julio A. Ramirez, Kenneth E. Palmer, Ruth Carrico, Forest W. Arnold, Donghoon Chung, Leslie A Wolf

The University of Louisville Journal of Respiratory Infections

An important feature of COVID-19, the disease produced by the new coronavirus SARS-CoV-2, is the high number of health care workers (HCWs) that acquire the disease. In an initial report of 138 patients hospitalized with COVID-19 pneumonia in China, 40 patients (29%) were HCWs. One reason why HCWs are at higher risk of acquiring COVID-19 is that some patients with COVID-19 are admitted to the hospital without the classical presentation and are therefore not tested for the disease early during hospitalization. Presently in the US, it is recommended to test for COVID-19 when physicians suspect the disease. This subjective approach …

Comorbidities Of Medicare Beneficiaries With Alzheimer's Disease In Florida, 2010, Tingting Hu, Henry J. Carretta

Florida Public Health Review

Comorbidity associated with Alzheimer’s Disease (AD) is highly prevalent but largely understudied. In this study, we sought to determine 1) the prevalence of AD by race (White, African American, and Hispanics); 2) 20 chronic conditions’ commonly comorbid with AD and the prevalence, mortality rate, and health care expenditure of common AD and comorbid condition by race. A sample of 86,875 Florida 2010 Medicare beneficiaries aged 65 and older with 12 months of fee-for-service (FFS) enrollment were used in this study. In the present analysis, the prevalence of AD was highest among elderly Hispanic beneficiaries. Among 20 chronic diseases, heart disease …

Coronavirus (“Covid-19”) Webpage, March 3, 2020, University Of Northern Iowa

UNI Response to COVID-19

A memo from the Director of Risk Management announcing the members of the COVID-19 Task Force to coordinate the response to the disease.

Synthetic Pesticides And Health In Vulnerable Populations: Agricultural Workers, Cynthia L. Curl, Meredith Spivak, Rachel Phinney, Luke Montrose

Public and Population Health Faculty Publications and Presentations

Purpose of Review: This review aims to summarize epidemiological literature published between May 15, 2018 and May 14, 2019 that examines the relationship between exposure to synthetic pesticides and health of agricultural workers.

Recent Findings: Current research suggests that exposure to synthetic pesticides may be associated with adverse health outcomes. Agricultural workers represent a potentially vulnerable population, due to a combination of unique social and cultural risk factors as well as exposure to hazards inherent in farm work. Pesticide exposure among agricultural workers has been linked to certain cancers, DNA damage, oxidative stress, neurological disorders, as well as respiratory, metabolic, …

Electronic Image Detectability Under Varying Illumination Conditions, Jeremy J. Miller

Theses and Dissertations

Light in the built environment plays an essential role in the vision and the health of humans through non-visual receptors in the eyes. Unfortunately, image analysts and other Air Force personnel who engage in the detection of objects on softcopy displays are often required to work in very dimly-lit or dark environments as higher illumination reduces the contrast of displayed information. Literature has shown that increases in light exposure improves circadian rhythm entrainment and reduces the negative health consequences of insufficient lighting. This research examines the effects of indoor lighting to determine if increases in ambient illumination or changes to …

Detection And Quantification Of Bacterial Species Important To Mental And Physical Health, David G. Leonard

Theses and Dissertations

The human gut microbiome contains an abundance of microorganisms which could influence mental health as well as physical health. These microorganisms produce chemicals which affect the brain and the body in various ways. Probiotic bacteria and yeasts have been studied to determine effects they have on mice, rats, and humans to illustrate the importance these microorganisms on health. Studies have shown that adding beneficial microorganisms to the human diet can have positive effects on mental and physical health, to include lessening symptoms of depression and anxiety, lessen gastrointestinal inflammation, displacing pathogens, and improving immunomodulatory response. A quantitative way to identify …

Learning From Transmasculine Experiences With Health Care: Tangible Inlets For Reducing Health Disparities Through Patient-Provider Relationships, Nickolas H. Lambrou, Katherine M. Cochran, Samantha Everhart, Jason D. Flatt, Megan Zuelsdorff, John B. O'Hara, Lance Weinhardt, Susan Flowers Benton, Carey E. Gleason

School of Public Health Faculty Research

Purpose: We examined health care experiences of transmasculine young adults to clarify factors contributing to mistrust in the health care system and identify tangible and modifiable means to address health disparities through improved patient-provider interactions. Thematic analysis highlights patterns within historical relationships between medical models and transmasculine embodiment, and provides guidance for health care clinicians, researchers, and policy makers to deliver competent services for transgender and gender diverse (TGD) individuals. Methods: The study team used qualitative methodology guided by interpretive phenomenological analysis. Semistructured interviews with 12 participants who self-identified as transmasculine were conducted, transcribed, and coded thematically. Results: Participants were …

Retrospective Cohort Study Of Pure Tone Audiometry Threshold Shifts From Ototoxic Substance, Continuous Noise, And Impulse Noise Exposures At Tinker Air Force Base From 2005 To 2019, Marc J. Blair

Theses and Dissertations

This retrospective cohort epidemiology study sought to establish the comparative risks and potential indicators of hearing loss associated with combinations of ototoxic substances, impulse noise, and continuous noise exposure. Currently, there is not an existing model or methodology in the Department of Defense (DoD) that joins occupational exposure data and pure tone audiometric data. After developing an integrated database model for Tinker Air Force Base, the largest of three depot installations within Air Force Material Command, 2,372 individuals were grouped into eight combinations of exposure groups with a minimum three years exposure duration to hazards. The incidence rates and relative …

Development And Characterization Of A Filter-Based Bioaerosol Sampler Capable Of Integration Into Small Unmanned Aerial Systems, Stephanie A. Ohms

Theses and Dissertations

In developing functional SUAS, performance characteristics that indicate system capability should be developed prior to initiating initial system design. Key performance parameters should be developed involving all system elements (including vehicle body, operator, ground station, sensor, and algorithm or processing module). A bioaerosol sampler designed specifically for the use in SUAS was characterized based on designated performance measures to determine overall effectiveness compared to traditional bioaerosol samplers. For a system with a goal of accurately identifying and quantifying areas of airborne biological contamination or surveying background levels for longitudinal studies, performance parameters such as weight of the vehicle with payload …

Evidence For Gene-Smoking Interactions For Hearing Loss And Deafness In Japanese American Families, Jia Y Wan, Christina Cataby, Andrew Liem, Emily Jeffrey, Trina M Norden-Krichmar, Deborah Goodman, Stephanie A Santorico, Karen L Edwards

Faculty, Staff and Student Publications

BACKGROUND: This study investigated the relationship between smoking and hearing loss and deafness (HLD) and whether the relationship is modified by genetic variation. Data for these analyses was from the subset of Japanese American families collected as part of the American Diabetes Association Genetics of Non-insulin Dependent Diabetes Mellitus study. Logistic regression with generalized estimating equations assessed the relationship between HLD and smoking. Nonparametric linkage analysis identified genetic regions harboring HLD susceptibility genes and ordered subset analysis was used to identify regions showing evidence for gene-smoking interactions. Genetic variants within these candidate regions were then each tested for interaction with …

Examining Measurement Reactivity In Daily Diary Data On Substance Use: Results From A Randomized Experiment, Anne Buu, Songshan Yang, Runze Li, Marc A Zimmerman, Rebecca M Cunningham, Maureen A Walton

Faculty, Staff and Student Publications

The debate about whether measurement reactivity exists in daily diary research on substance use is still unsettled due to the issues of study design and statistical methodology. This study proposes a time-varying effect model (TVEM) that characterizes the trajectory of substance use behaviors with nonparametric functions determined by the data rather than imposes presumed parametric functions. It also allows researchers to investigate the effect of measurement reactivity on not only the likelihood of using substances but also the amount of substance use. The TVEM was applied to analyze diary data on alcohol and marijuana use collected from an experiment, which …

Unpredictable Maternal Behavior Is Associated With A Blunted Infant Cortisol Response, Amanda N. Noroña-Zhou, Alyssa Morgan, Laura M. Glynn, Curt A. Sandman, Tallie Z. Baram, Hal S. Stern, Elyssia Poggi Davis

Psychology Faculty Articles and Research

Background

Dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis is associated with poor physical and mental health. Early-life adversity may dysregulate cortisol response to subsequent stress. This study examines the association between patterns of maternal behavior and infant stress response to a challenge. Specifically, we test whether infant exposure to unpredictable maternal sensory signals is related to the cortisol response to a painful stressor.

Method

Participants were 102 mothers and their children enrolled in a longitudinal study. Patterns of maternal sensory signals were evaluated at 6 and 12 months during a 10-min mother–infant play episode. Entropy rate was calculated as a quantitative …

Brain Candy: Wayne State University School Of Medicine Journal Of Arts And Culture, Special Edition: Reflections, Wayne State University School Of Medicine Gold Humanism Honor Society

Gold Humanism Honor Society

Brain Candy collects poetry, nonfiction essays, short fiction, photographs, and drawings to shed light on the creative process in medicine, the city of Detroit, and the experiences of health care providers. Each issue features submissions from medical students, physicians, and School of Medicine staff. With generous funding support from an alumnus, Dr. Thomas Janisse, Class of 1975, the journal was started in print in 2016.

Keberadaan Jentik Aedes Aegypti Dan Aedes Albopictus Berdasarkan Karakteristik Kontainer Di Sekolah Dasar, Keluharan Duren Sawit, Jakarta Timur, Tahun 2018, Bobby Ilham Ramadhan, Umar Fahmi Achmadi

Jurnal Nasional Kesehatan Lingkungan Global

Background. Kelurahan Duren Sawit is the highest kelurahan found cases of PE (+) and death cases of DHF in Primary school-aged children in Kecamatan Duren Sawit. This study aimed to see larva Aedes aegypti and Aedes albopictus based on container chracteristics include types, materials, volume, water condition and color in SD, Kelurahan Duren Sawit. Based on the identified container characteristics, then we can know the potential level of longing for the presence of larva Ae. aegypti and Ae. albopictus. Method. This study uses primary data by looking all containers in Kelurahan Duren Sawit Primary School. The design study was cross-sectional, …

Analisis Kadar Kadmium Dan Beberapa Parameter Kunci Pada Air Lindi Di Tempat Pengolahan Sampah Terpadu (Tpst) Bantar Gebang Tahun 2018, Inas Fadhilah, Laila Fitria

Jurnal Nasional Kesehatan Lingkungan Global

Background. Waste which accumulates in landfills can lead to contamination of the environment and risk to the health of the local population. One cause of contamination is leachate. Integrated Waste Treatment Plant (TPST) Bantar Gebang treated leachate water in Waste Water Management Site (IPAS). Method. This research was conducted to know leachate water treatment efficiency and to know cadmium content and some key parameters. This study was conducted by sampling on inlet, outlet and surface water on different days. This study also added measurement data conducted by Bantar Gebang. The results were analyzed and compared to the regulatory standards in …

Determinants Of Stunted Children In Indonesia: A Multilevel Analysis At The Individual, Household, And Community Levels, Febri Wicaksono, Titik Harsanti

Kesmas

This study aimed to examine the risk factors of childhood undernutrition in Indonesia. Determinants of childhood stunting were examined by using the 2013 Indonesia Basic Health Research Survey dataset. A total of 76,165 children aged under 5 years were included in this study. The analysis used multivariate multilevel logistic regression to determine adjusted odds ratios (aORs). The prevalence of stunting in the sample population was 36.7%. The odds of stunting increased significantly among the under-five boys, children living in slum area, and the increase of household member (aOR = 1.11, 95 %CI: 1.06–1.15; 1.09, 95%CI: 1.04–1.15; and 1.03, 95%CI: 1.02–1.04 …

Evidence To Support Hiv Prevention For Adolescent Girls And Young Women (Agyw) And Their Male Partners: Results From Malawi Dreams Studies With Agyw, Male Partners Of Agyw, Men Living With Hiv, And Program Implementing Partners, Nanlesta Pilgrim, Victor Mwapasa, Effie Chipeta, Wanangwa Chimwaza Manda, Nrupa Jani, Tracy Mcclair, Lyson Tenthani, Sanyukta Mathur

HIV and AIDS

Project SOAR, led by the Population Council, in partnership with the Center of Reproductive Health at the University of Malawi College of Medicine, conducted a research portfolio to generate evidence to reduce HIV risk among adolescent girls and young women (AGYW) and their male partners. The objectives of this implementation research were to generate evidence for describing HIV-related risk factors among AGYW; assess the extent to which the overall DREAMS project contributed toward the goal of reducing HIV risk among AGYW; and understand the characteristics of male partners and how to link them to HIV services, as well as retain …

Strengthening Capacity For Assessment Of Hiv-Related Data Needs Among Key Populations To Inform Evidence-Based Responses, Amrita Rao, Nikita Viswasam, Stefan Baral

HIV and AIDS

Defining the HIV prevention and treatment needs of key populations who have disproportionate HIV acquisition and transmission risks has been particularly difficult in the context of generalized HIV epidemics where less attention has historically been placed on the HIV prevention and treatment needs of these groups. There is a gap in our understanding of the specific needs of—and ultimately the investment case for the added value of supporting—disproportionately burdened key populations in these settings. In response to this gap, Johns Hopkins University under the United States Agency for International Development (USAID)-funded Project SOAR implemented a project in partnership with collaborators, …

Characterization Of Urinary Concentrations Of Heavy Metals Among Socioeconomically Disadvantaged Black Pregnant Women, Inkyu Han, Kristina W Whitworth, Xueying Zhang, Masoud Afshar, Pamela D Berens, Elaine Symanski

Faculty, Staff and Student Publications

The objective of this study was to characterize exposures to metals using biological samples collected on socioeconomically disadvantaged black pregnant women. We obtained 131 anonymous urine samples provided by black pregnant women visiting a Medicaid-serving prenatal clinic in Houston, TX, from March 27, 2017 to April 11, 2017. We analyzed urine samples for 15 metals including cadmium (Cd), arsenic (As), lead (Pb), and nickel (Ni) and for creatinine and cotinine. We found that median concentrations of zinc (Zn), selenium (Se), and aluminum (Al) among black pregnant women in this study were 1.5 to 3 times higher than levels reported among …

A Qualitative Study Of The Genesis Of A Community Food Pantry For Students Of Higher Education, Elizabeth Fast

Theses and Dissertations

Objective: The purpose of this case study was to understand the development of a food pantry for students of higher education at a Midwestern university.

Methods: The researchers recruited 11 individuals on the food pantry’s board using a snowball sampling method to participate in a 30-minute interview about the food pantry’s development from conception to implementation. The interviews were transcribed and then qualitatively analyzed by two researchers. The researchers coded interviews separately to identify frequent commonalities between participant responses. The researchers then organized the codes into themes, which were then summarized in the results of this study.

Results: The first …

Pesticides Decrease Bacterial Diversity And Abundance Of Irrigated Rice Fields, Michael Onwona-Kwakye, Kimberly Plants-Paris, Kadiatou Keita, Jessica Lee, Paul J Van Den Brink, Jonathan N Hogarh, Charles Darkoh

Faculty, Staff and Student Publications

Bacteria play an important role in soil ecosystems and their activities are crucial in nutrient composition and recycling. Pesticides are extensively used in agriculture to control pests and improve yield. However, increased use of pesticides on agricultural lands results in soil contamination, which could have adverse effect on its bacterial communities. Here, we investigated the effect of pesticides commonly used on irrigated rice fields on bacterial abundance and diversity. Irrigated soil samples collected from unexposed, pesticide-exposed, and residual exposure areas were cultured under aerobic and anaerobic conditions. DNA was extracted and analysed by 16S rRNA sequencing. The results showed overall …