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Articles 3271 - 3300 of 26282
Full-Text Articles in Entire DC Network
Invertebrate Pests Of Endophyte-Infected And Endophyte Free Perennial Ryegrass Swards In Northern New Zealand, R A. Prestidge, R N. Watson, E R. Thom
Invertebrate Pests Of Endophyte-Infected And Endophyte Free Perennial Ryegrass Swards In Northern New Zealand, R A. Prestidge, R N. Watson, E R. Thom
IGC Proceedings (1977-2023)
Plots of endophyte-infected and endophyte-free perennial ryegrass (Lollum perenne) cv. Ellett and white clover (Trifolium repens) cv, Grasslands Pitau were established in autumn 1990 by direct drilling into pastures previously sprayed with glyphosate herbicide. Plots were rotationally grazed by dairy cows. Insect pests were sampled by soil coring and extraction, and plant pathogenic nematodes by root staining and examination. Ryegrass tiller density and the proportion of ryegrass in the swards were-significantly less in November, March and May on plots sown with endophyte-free ryegrass c_ompared with endophyte-infected ryegrass. Conversely, the propo!'tion __ of white clover was greater on endophyte-free …
Invertebrate Pests Associated With Italian Ryegrass In Northern New Zealand, R A. Prestidge, R N. Watson, E R. Thom
Invertebrate Pests Associated With Italian Ryegrass In Northern New Zealand, R A. Prestidge, R N. Watson, E R. Thom
IGC Proceedings (1977-2023)
Damage threshold levels were established for adult Argentine stem weevil (Listronotus bonariensis (Kuschel)) feeding on Italian ryegrass (Lolium multiflorum Lam;) cv, Concord seedlings in a glasshouse pot trial. Population densities of 35-70/m1 reduced seedling survival by 34% and herbage yield after 28 days growth by 35%. A field trial was established In autumn 1991 by sowing Concord in early February, in late February and in March into areas previously treated with glyphosate herbicide, Insecticide application increased ryegrass tiller density by 36% from 2339/m' (April 1991) and herbage accumulation by 43% from 1.4 t DM/ha only up to the …
Screening For Biological Control Agents Of Root Pathogens Of Pasture Legumes, R A. Skipp, F S. Hay
Screening For Biological Control Agents Of Root Pathogens Of Pasture Legumes, R A. Skipp, F S. Hay
IGC Proceedings (1977-2023)
Fungi isolated from roots of legumes from pasture soil were applied to seeds of red clover (Trifolium pratense L.) on water agar in Petri plates in a seedling screening test designed to detect antagonism to the fungal pathogens Fusarium solani, Trichrocadium basicola and a Pythium sp. Isolates of Triclroderma koningii, T. /ramatum, a binucleate Rhizoc1011ia sp. or an unidentified basidiomycete protected seedling roots from damage by one or more of the fungal pathogens. Fungi isolated from cysts of the plant-parasitic nematode Helerodera trifolii obtained from pasture soil were tested for their ability to infect eggs of H. trifolii …
Effects Of Three Viruses On Productivity Of Annual Pasture Legume Swards, R A. C Jones
Effects Of Three Viruses On Productivity Of Annual Pasture Legume Swards, R A. C Jones
IGC Proceedings (1977-2023)
The effects of 3 non-persistently aphid-transmitted viruses on the productivity of swards of annual pasture legumes were investigated in 1988-91. Virus-host combinations studied were alfalfa mosaic virus (AMV) in burr medic (Medicago polymorplia), cucumber mosaic virus (CMV) in subterranean clover (Trifo/i111n subterra11eum) and bean yellow mosaic virus (BYMV) in subterranean clover. Swards were mown to simulate grazing, grazed or left undefoliated. Herbage (dry weight) and seed production were decreased significantly by infection with all three virus-host combinations. However, the extent of losses vnried with extent of virus spread, cultivar, site and season. Decreased seed yield resulted from both fewer and …
Burial Of Surface-Applied Lime By Earthworms In South Australian Pastures, G H. Baker, P. M.L Williams, V J. Barrett, P J. Carter
Burial Of Surface-Applied Lime By Earthworms In South Australian Pastures, G H. Baker, P. M.L Williams, V J. Barrett, P J. Carter
IGC Proceedings (1977-2023)
The ability of earthworms, Aporrectodea trapewides, A. caliginosa, A. rosea (Lumbricidae) and Gemascolex walkeri (Megascolecidae), to bury surface-applied lime and offset soil acidity was investigated in 2 pastures in the Mount Lofty Ranges, South Australia, In one pasture, A. trapewides increased pH by 0.8 of a unit at 4-6 cm depth. A. caliginosa also increased soil pH compared with controls, but A. rosea and G. walkeri had no influence, A. trapewides is suggested as a valuable addition to acid pastures, if not already present,
Effects Of Fungicide Seed Treatments On Germination And Establishment Of Sainfoin And Other Legumes, Nan Zhi Biao, Liu Zhao Hui, R A. Skipp
Effects Of Fungicide Seed Treatments On Germination And Establishment Of Sainfoin And Other Legumes, Nan Zhi Biao, Liu Zhao Hui, R A. Skipp
IGC Proceedings (1977-2023)
Treatment of seed with thiram or thiophanate methyl at a rate of 3 g ai/kg seed increased germination of crownvetch (Coro11illa varia L.), milkvetch (Astragalus ads11rge11s Pall.), sainfoin (011obrycl1is viciifolia Scop.) and yellow sweet clover (Melilotus officinalis (L.) Lam.) in laboratory tests. The magnitude of the increases varied with fungicide and species. The greatest increase in germination relativ!) to that of untreated seed was 115% for sainfoin dusted with thiophanate methyl. Thiophanate methyl increased field emergence of sainfoin at 4 and 8 weeks after sowing by 41% and 55% respectively, and at 8 weeks increased the dry weights of shoots …
Pastures In Crop Rotations Enhance Earthworm Populations In Southern Australia, John C. Buckerfield
Pastures In Crop Rotations Enhance Earthworm Populations In Southern Australia, John C. Buckerfield
IGC Proceedings (1977-2023)
The effects of crop rotations on earthworm populations in seasonally dry arable lands was assessed on long-term field trials at 2 sites with an annual rainfall of 450-500 mm, In cropping and sheep-grazing areas of southern Australia. Earthworm densities were substantially higher in rotations of cereals with a volunteer-pasture phase than under continuous cropping with cereals and grain legumes. The degree and frequency of soil disturbance associated with cropping has been shown to influence earthworm numbers: trial plots In which crops were sown with minimal cultivation, in alternate years, supported earthworm populations 2-3 times higher than those conventionally cultivated and …
Costs And Benefits Of Controlling Grasshoppers On Rangeland, E W. Huddleston, R Sanderson, L A. Torell, J H. Davis, J G. Watts
Costs And Benefits Of Controlling Grasshoppers On Rangeland, E W. Huddleston, R Sanderson, L A. Torell, J H. Davis, J G. Watts
IGC Proceedings (1977-2023)
Ultra low volume (ULV) aerial application of malathion at 0.58 I/ha is the common method of rangeland grasshopper control in the USA. Aircraft often are unavailable or too expensive for treating small infestations; therefore, air-assist ground equipment was adjusted 10 apply the ULV rate from a small truck. In winds of 2.2 m/sec or greater, control with the ground equipment was equivalent to that expected from aircraft. The low spray volume, low sprayer cost and use of a small truck have increased the benefit/cost ratio. Using results from this research and site-specific estimates of control costs and forage value, the …
The Rodent Pests Of Pasture And Their Control In China, Xixian Hou, Weihui Dong, Yanlin Zhou
The Rodent Pests Of Pasture And Their Control In China, Xixian Hou, Weihui Dong, Yanlin Zhou
IGC Proceedings (1977-2023)
Rodent damage is increasingly recognised as a major problem for grasslands of China rodents damage up to an estimated 33 million ha each year, There are two reasons for rodent damage in grassland: one is overgrazing, which causes degeneration of grassland and provides good habitats for rodents; another is the ecological characteristics of rodents. A rodent control programme has been followed for years in China, and includes: (1) studies on the ecology of major rodent pests in pasture, (2) prediction of population, (3) setting up control indexes paying attention to economic benefits, (4) tightening up grassland management and developing integrated …
Stylo Host Heterogeneity For Anthracnose Management, S Chakraborty, A N. Petitt, R M. Boland, S Low Choy, D F. Cameron, J A.G Irwin, R D. Davis
Stylo Host Heterogeneity For Anthracnose Management, S Chakraborty, A N. Petitt, R M. Boland, S Low Choy, D F. Cameron, J A.G Irwin, R D. Davis
IGC Proceedings (1977-2023)
Small plots of 3 different mixtures of Stylosallllles. scabra genotypes were established within background paddocks of either pure cv. Seca, or a mixture of 6 S. scabra genotypes including Seca, to study anthracnose development in the three mixtures under exogenous inoculum pressures arising from the two backgrounds. There was no difference in disease severity between the background paddocks after the first year. In the small plots, severity of nearest neighbours significantly influenced anthracnose severity on the partially resistant accession 55860 in the first year; this effect disappeared as the disease became more widespread throughout the plot in later years. Replacing …
Increased Resistance To Sclerotinia Crown And Stem Rot Of Alfalfa Through Recurrent Selection Of Surviving Plants In Inoculated Field Plots, M Kanbe, F Fujimoto, S Inami
Increased Resistance To Sclerotinia Crown And Stem Rot Of Alfalfa Through Recurrent Selection Of Surviving Plants In Inoculated Field Plots, M Kanbe, F Fujimoto, S Inami
IGC Proceedings (1977-2023)
ABSTRACT Breeding alfalfa (Medicago saliva L.) for resistance to Sclerotinia crown and stem rot (SCR; causal agent Sclerotitivia trifoliorum Eriks.), which is a serious disease in Japan, was started in 1983. Increased resistance to this disease has been examined after repeated selected of surviving plants from artificially inoculated field plots to the 5th generation. The strains selected for SCR resistance showed higher resistance than Tachiwakaba, which was the main breeding material. The effectiveness of selection judged by the survival rate of progenies was low in the early selection generations but high in the later ones, It is considered that …
Biological Control Of Alfalfa Aphids In Japan Using Coccinella Septempunctata Brucki, Keiichi Takashi
Biological Control Of Alfalfa Aphids In Japan Using Coccinella Septempunctata Brucki, Keiichi Takashi
IGC Proceedings (1977-2023)
The oviposition, predation, and survival abilities of Coccinella septempuncrara bmcki Mulsanl (lady beetle), a natural enemy of alfalfa aphids (Acyrtllosyphon kondoi Shinji), increased as temperatures rose in laboratory tests. The second-instar larvae were released into cages in alfalfa (Medicago saliva L.) fields in spring 1991. One month later, alfalfa plants in the control cages without larvae showed much lower yield; however, no damage was observed in cages in which larvae had been released. There is a strong probability that alfalfa aphids can be controlled by the timely release of lady beetle larvae in the field.
Twas Facilitates Gene-Scale Trait Genetic Dissection Through Gene Expression, Structural Variations, And Alternative Splicing In Soybean, Delin Li, Qi Wang, Yu Tian, Xiangguang Lyv, Hao Zhang, Huilong Hong, Huawei Gao, Yan-Fei Li, Chaosen Zhao, Jiajun Wang, Ruizhen Wang, Jinliang Yang, Bin Liu, Patrick S. Schnable, James C. Schnable, Ying-Hui Li, Li-Juan Qiu
Twas Facilitates Gene-Scale Trait Genetic Dissection Through Gene Expression, Structural Variations, And Alternative Splicing In Soybean, Delin Li, Qi Wang, Yu Tian, Xiangguang Lyv, Hao Zhang, Huilong Hong, Huawei Gao, Yan-Fei Li, Chaosen Zhao, Jiajun Wang, Ruizhen Wang, Jinliang Yang, Bin Liu, Patrick S. Schnable, James C. Schnable, Ying-Hui Li, Li-Juan Qiu
Department of Agronomy and Horticulture: Faculty Publications
A genome-wide association study (GWAS) identifies trait-associated loci, but identifying the causal genes can be a bottleneck, due in part to slow decay of linkage disequilibrium (LD). A transcriptome-wide association study (TWAS) addresses this issue by identifying gene expression–phenotype associations or integrating gene expression quantitative trait loci with GWAS results. Here, we used self-pollinated soybean (Glycine max [L.] Merr.) as a model to evaluate the application of TWAS to the genetic dissection of traits in plant species with slow LD decay. We generated RNA sequencing data for a soybean diversity panel and identified the genetic expression regulation of 29 …
Scaling Individual Tree Transpiration With Thermal Cameras Reveals Interspecies Differences To Drought Vulnerability, Mostafa Javadian, Donald M. Aubrecht, Joshua B. Fisher, Russell L. Scott, Sean P. Burns, Jen L. Diehl, J. William Munger, Andrew D. Richardson
Scaling Individual Tree Transpiration With Thermal Cameras Reveals Interspecies Differences To Drought Vulnerability, Mostafa Javadian, Donald M. Aubrecht, Joshua B. Fisher, Russell L. Scott, Sean P. Burns, Jen L. Diehl, J. William Munger, Andrew D. Richardson
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Understanding tree transpiration variability is vital for assessing ecosystem water-use efficiency and forest health amid climate change, yet most landscape-level measurements do not differentiate individual trees. Using canopy temperature data from thermal cameras, we estimated the transpiration rates of individual trees at Harvard Forest and Niwot Ridge. PT-JPL model was used to derive latent heat flux from thermal images at the canopy-level, showing strong agreement with tower measurements (R2 = 0.70–0.96 at Niwot, 0.59–0.78 at Harvard at half-hourly to monthly scales) and daily RMSE of 33.5 W/m2 (Niwot) and 52.8 W/m2 (Harvard). Tree-level analysis revealed species-specific responses to …
Curating And Validating Universally Applicable Primers For Efficient Its2-Based Dna Barcoding Across Plant Taxa, Ajay R Bhat, Subrahmanya Hegde, Supriya S Kammar, Mb Muthamma, Gaurav Mudgal, Prashanth Babu H, Rajulu Charukesi, Thotegowdanapalya C Mohan
Curating And Validating Universally Applicable Primers For Efficient Its2-Based Dna Barcoding Across Plant Taxa, Ajay R Bhat, Subrahmanya Hegde, Supriya S Kammar, Mb Muthamma, Gaurav Mudgal, Prashanth Babu H, Rajulu Charukesi, Thotegowdanapalya C Mohan
International Journal of Health and Allied Sciences
Abstract: Rapid and accurate identification of plant species increasingly seeks to employ molecular techniques. The ITS2 region is highly regarded in DNA barcoding due to its short length and ease of sequencing, making it an ideal candidate for species identification
Ai And Future-Making: Design, Biases, And Human-Plant Interactions, Maliheh Ghajargar
Ai And Future-Making: Design, Biases, And Human-Plant Interactions, Maliheh Ghajargar
Art Faculty Articles and Research
Design researchers and practitioners are turning to generative AI (genAI) to support activities such as ideation and concept development in pursuit of preferred futures. At the same time, genAI is known to have biases, which prompts questions about how these biases might adversely affect design practices. In the domain of sustainable HCI, with its recent trends in human-nature interactions and more-than-human design, the question can be further refined into whether and how genAI biases might perpetuate anthropocentric biases that these practices are increasingly seeking to confront. In the present research, we conducted three workshops, focusing on genAI for human-plant interactions; …
"The Words We Do Not Yet Have." A Creative Inquiry Into Human-Plant Relationships, Maliheh Ghajargar
"The Words We Do Not Yet Have." A Creative Inquiry Into Human-Plant Relationships, Maliheh Ghajargar
Art Faculty Articles and Research
Climate change, loss of plant biodiversity, and ocean pollution signal the drastic changes in our ecology that call us to attend to the needs of more than human forms of life on Earth. Sustainable design and HCI research are responding to this call by offering methods and approaches to design more sustainable products and systems and recently, more than human design is building momentum. This agenda seeks to reform traditional design processes by decentering the creative agency of the dominant socio-economical group of humans and foregrounding those of diverse Others. In this paper, I focus on plants as a nonhuman …
Genetic Diversity Of Soybean Dwarf Virus In Two Regions Of Mainland Australia, Benjamin S. Congdon, Murray Sharman, Monica Kehoe
Genetic Diversity Of Soybean Dwarf Virus In Two Regions Of Mainland Australia, Benjamin S. Congdon, Murray Sharman, Monica Kehoe
Biosecurity Research Articles
Soybean dwarf virus (SbDV; family Tombusviridae, genus Luteovirus, species Luteovirus glycinis) is an RNA plant virus that is transmitted solely by aphids in a persistent, circulative and non-propagative manner. SbDV causes significant losses in cultivated Fabaceae, especially in subterranean clover (Trifolium subterraneum) pastures of mainland Australia. SbDV isolates are classified into four phenotypically distinguishable strains: YP, YS, DP, and DS. Y and D strains differ primarily in their host range, and P and S strains in their primary vector species. Genetically, Y and D strains separate into two clades in every genomic region except for …
Latticed Stinkhorn, Emilee G. Seigworth
Latticed Stinkhorn, Emilee G. Seigworth
PANDION: The Osprey Journal of Research and Ideas
Artist Statement
Latticed Stinkhorn is a watercolor piece originally created for the special gallery of the 2023 Scientific Illustration course at the University of North Florida. It was inspired by my enduring fascination with the world of fungi, particularly specimens of the family Phallaceae. This watercolor piece offers a simple perspective of Clathrus ruber, a species commonly referred to as Latticed Stinkhorn. Like many members of the family, the reproductive fruiting body first surfaces encased in a cream-colored soft shell with a texture comparable to a leathery reptile egg. As the body ages, the initial shell disintegrates to reveal a …
Growth Of Passionflower (Passiflora Incarnata) On A Green Roof Using Commercial Fertilizer Vs. Natural Compost, Siobhan Day, Corinne Parker, Carmyn Harrison, Lauren York
Growth Of Passionflower (Passiflora Incarnata) On A Green Roof Using Commercial Fertilizer Vs. Natural Compost, Siobhan Day, Corinne Parker, Carmyn Harrison, Lauren York
Science University Research Symposium (SURS)
Passionflower (Passiflora incarnata), Tennessee’s state wildflower, is valued for its ecological role as a pollinator plant and its historical significance to the Cherokee, making it an ideal candidate for green roofs that support biodiversity and urban sustainability. This study examined the feasibility of growing passionflower on a green roof and tested whether commercial fertilizer (Miracle-Gro) or natural compost would lead to greater growth compared to no fertilizer. Twelve plants were divided equally into three treatment groups: control, Miracle-Gro, and compost, with growth measurements taken weekly from September to November. Results suggest that passionflowers treated with Miracle-Gro achieved the most growth, …
Fire And Ant Interactions Mediated By Honeydew And Extrafloral Nectar In An Australian Tropical Savanna, Fernanda M. P. Oliveira, Carlos H. F Silva, Melinda L. Moir, Inara R. Leal, Alan N. Andersen
Fire And Ant Interactions Mediated By Honeydew And Extrafloral Nectar In An Australian Tropical Savanna, Fernanda M. P. Oliveira, Carlos H. F Silva, Melinda L. Moir, Inara R. Leal, Alan N. Andersen
Natural Resources Research Articles
Fire is a major disturbance affecting ecosystems globally, but its impact on mutualisms has received minimal attention. Here, we use a long-term field experiment to investigate the impact of different fire regimes on globally important ant–honeydew and ant–extrafloral nectar (EFN) mutualistic interactions in an Australian tropical savanna. These interactions provide ants with a key energy source, while their plant and hemipteran hosts receive protection services. We examined ant interactions on species of Eucalyptus (lacking EFNs) and Acacia (with EFNs) in three replicate plots each of burning every 2 and 3 years early in the dry season, burning late in the …
2024 Fall Newsletter, Plaster Creek Stewards
2024 Fall Newsletter, Plaster Creek Stewards
Newsletters
Highlights:
Project GreenER Returns in 2025
Sustainable Business of the Year
PCS Is Showing Up Around the Community
Botanical Highlight: Discovery of a New Kent County Native Grass
Fall Event Furthers New Tree Planting Initiatives
Plaster Creek: Unsalted?
Making Connections at Calvin
Parkway Pocket Garden Maintenance Booklet, Plaster Creek Stewards
Parkway Pocket Garden Maintenance Booklet, Plaster Creek Stewards
Print Materials
Parkway pocket gardens are designed for areas between the sidewalk and street, but without a basin and curb-cut. By replacing lawn in parkways with native plants, it can soak up rainwater that falls on the gardens, provide habitat and a food source for pollinators and birds, and increase biodiversity in your neighborhood. This maintenance guide covers the Michigan native plants that may be found in a parkway pocket garden, maintenance recommendations, and weeds you may encounter.
Cold Stress Mechanisms In Asian Rice Subspecies And Overexpression Of Osubc7 For Enhancing Rice Cold Tolerance, Huy Minh Phan
Cold Stress Mechanisms In Asian Rice Subspecies And Overexpression Of Osubc7 For Enhancing Rice Cold Tolerance, Huy Minh Phan
Dissertations (1934 -)
By 2050, the projected 9.7 billion global population will increase food demand, especially for staple crops. Climate change, with extreme temperature fluctuations, heavily impacts cold-sensitive Asian rice (Oryza sativa L.) grown in tropical and subtropical areas. Therefore, understanding the response mechanisms of two Asian rice subspecies, japonica and indica, which have distinctive tolerance to cold stress, becomes important for improving crop cold tolerance. Hence, this study tested our hypotheses to address how japonica can tolerate cold exposure better than indica: (1) japonica selectively adjusts antioxidant activities to counter reactive oxygen species (ROS), while indica rapidly increases antioxidant activities early; (2) …
Of Attachment And Connection: Auxin Signaling In The Cambium Promotes Successful Plant Grafting, Janlo M. Robil
Of Attachment And Connection: Auxin Signaling In The Cambium Promotes Successful Plant Grafting, Janlo M. Robil
Biology Faculty Publications
No abstract provided.
Field-Based Courses Still Matter, But Not Like They Used To, Christopher T. Martine
Field-Based Courses Still Matter, But Not Like They Used To, Christopher T. Martine
Faculty Journal Articles
Increasingly, field-based courses are providing the first real opportunities for students to experience nature in meaningful ways. As a baseline, even before the content delivery and the graded assessments, this is already enough to change the way a person feels in the world and to spark an appreciation for the life around them.
(Part of a special feature on education in which recent winners of the Botanical Society of America's Charles E. Bessey Award share teaching philosophies and strategies.)
Oxidative Stress Responses In Arabidopsis Thaliana Plants To Uv-B Irradiation, Bolaji Olalere
Oxidative Stress Responses In Arabidopsis Thaliana Plants To Uv-B Irradiation, Bolaji Olalere
Theses
The depletion of the stratospheric ozone layer has led to increased levels of harmful UV-B radiation reaching the Earth's surface, posing significant challenges for plant health and development. This study investigates the oxidative stress responses in Arabidopsis thaliana when exposed to UV-B irradiation. Arabidopsis thaliana, a well-established model organism, is particularly sensitive to UV-B, making it ideal for understanding the effects of UV-B-induced oxidative stress. UV-B radiation, a mutagenic component of sunlight, disrupts key metabolic, molecular, and developmental processes, leading to DNA damage, impaired metabolism, and morphological changes. This research aims to explore how UV-B exposure affects the activities of …
Control Small Weeds In Crops, Department Of Primary Industries And Regional Development, Western Australia
Control Small Weeds In Crops, Department Of Primary Industries And Regional Development, Western Australia
Grains and other field crops factsheets
In an integrated weed management program, control of weeds should occur in the fallow, pre-sowing, early post-emergent, and in-pasture phases. Weed control can be achieved through killing weeds (particularly seedlings) with cultivation, herbicides, targeted weed control in wide-row cropping, targeted control of small patches of weeds, and biological control tactics.
Managing Frost Risk, Department Of Primary Industries And Regional Development, Western Australia
Managing Frost Risk, Department Of Primary Industries And Regional Development, Western Australia
Grains and other field crops factsheets
Frost occurs on clear nights in early spring when the air temperature drops to 2°C or less. Crop damage from frost may occur at any stage of development but is most damaging at or around flowering.
Frost is difficult to manage, and damage will be unavoidable in some seasons. Risk management options proven to reduce frost risk include paddock zoning, crop and variety selection, time of sowing, stubble management and managing inputs. The greatest gains result from in paddock choice/zoning, crop and variety choice, and manipulating the canopy.
Zadoks Growth Scale, Department Of Primary Industries And Regional Development, Western Australia
Zadoks Growth Scale, Department Of Primary Industries And Regional Development, Western Australia
Grains and other field crops factsheets
Zadoks growth scale is a 0-99 scale of development that is recognised internationally for research, advisory work, and farm practice, particularly to time the application of chemicals and fertilisers.