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They Forgot We Were Seeds: Critical Counter-Narratives Supporting Culturally Informed Community Seed Work, Florentina Rodriguez

Antioch University Dissertations & Theses

This Professional Innovation dissertation utilizes Critical Counter-Narrative both as research methodology to generate narrative reflections and as praxis for liberatory transformation. Through research vignettes adapted from the author’s experience with critical collaborative autoethnographic seed work, this study produced critical counter-narratives aimed at addressing inequities in small-scale seed systems. These counter-narratives were incorporated into curriculum materials synthesized as an educational toolkit for a professional research

sister-project: the new Culturally Informed Community Seed Pledge and Code of Ethics (CICSP/COE). This project seeks to address disparity and extractive practices; outline relational pathways for reciprocity and respecting communities of origin; and provide evaluation metrics …

Characterization Of Major Foliar Fungal Species Affecting Cucurbits In South Dakota, Janani Perera Waduwarage Dona

Electronic Theses and Dissertations

Cucurbit crops comprise the largest area in vegetable production in South Dakota (USDA 2022). Information on the prevalence, distribution, and fungicide sensitivity of their fungal pathogens are unknown. This study characterized the major foliar fungal species affecting cucurbits in South Dakota through a 2-year, state-wide survey. Between 2023 and 2024, 789 leaves were recovered from pumpkin, cucumber, watermelon, squash, and bottle gourd. Alternaria spp. (70.72% of leaves) Fusarium spp. (6.84%), Podosphaera xanthii (4.18%), both Alternaria spp. and Fusarium spp. (3.42%), and both Alternaria spp. and Podosphaera xanthii (1.90%), Stagonosporopsis spp. (1.27%) and other genera of fungi (4.94%) were identified using …

Enhancing Alfalfa Persistence In Rangelands: Evaluating Morphological Traits Of Medicago Sativa Subsp. Falcata Germplasm, Abdulallah Mohammed Alomair

Electronic Theses and Dissertations

Rangelands cover nearly half of the Earth’s surface, representing about 54% (79 million km2), and occur in different types and climates worldwide. Alfalfa (Medicago sativa L.) is a major component in rangelands and covers about (320 thousand km2), representing about 0.4% of total global rangeland area. As a critical perennial legume crop, alfalfa can improve soil health by fixing approximately 336 kg/ha/year of nitrogen and sequestering up to 614 kg/ha/year of carbon, while enhancing livestock production due to its high content level of vitamins, magnesium, minerals, fiber, and protein. However, environmental stresses such as drought, freezing, and grazing limit alfalfa …

Epidemiological And Diagnostic Approaches For Soybean Cyst Nematode In South Dakota, Nabina Karki

Electronic Theses and Dissertations

Soybean cyst nematode (SCN), Heterodera glycines (HG) Ichinohe, is an endoparasitic nematode and one of the major yield-limiting pathogens of soybean (Glycine max L.) in the United States. Soybean cyst nematode alone accounts for more than $1.5 billion economic loss annually in North America. First reported in the U.S.A. in 1954 in New Hanover County, North Carolina and later in 1995 in Union County, South Dakota. Prior to this study, SCN has spread in 34 counties in the state. As a soil pathogen, which can adapt to most soil conditions under soybean production and beside soybean, it is hosted on …

Evaluation Of Two Stem Boring Insects In South Dakota: A Survey Of Wheat Stem Sawfly And Determining The Impact Of Planting Date On Dectes Stem Borer Infestations In Sunflowers, Khuma Kumari Bhusal

Electronic Theses and Dissertations

Wheat, Triticum aestivum L., and sunflower, Helianthus annuus L., are major crops in South Dakota that are threatened by stem-boring insect pests capable of reducing yield and harvestability. In wheat, the wheat stem sawfly, Cephus cinctus Norton, has long been recognized as a destructive pest in the Northern Great Plains, yet its actual distribution within South Dakota remained uncertain. To determine its presence, a two-year survey was conducted across the state from 2023–2024, during which one to four wheat fields per county were sampled using standardized sweep net transects. Wheat stem sawflies were collected only from Harding and Perkins Counties …

Cover Cropping Strategies For Improving Weed Suppression, Yashar Askarzadeh

Electronic Theses and Dissertations

Cover crops (CCs) play an essential role in sustainable agriculture by providing multiple ecosystem services. However, adoption remains low due to concerns about economic returns, management complexity, and potential yield reductions in cash crops. Applying CCs uniformly across fields without accounting for spatial variability can result in inconsistent outcomes. A precision cover cropping system (PCCS), which incorporates site-specific management zones (MZs), offers a more targeted approach to improving performance while minimizing costs and uncertainties. This study explores the interaction between CCs and field variability through complementary field and greenhouse experiments. The field experiment demonstrates that field heterogeneity significantly influences CC …

Isolation, Characterization & Identification Of Cultivable Field Pea Seed Bacteria In South Dakota, Diana Wairimu Njuguna

Electronic Theses and Dissertations

Pea (Pisum sativum L.) is a legume, cultivated on over 25 million acres, particularly in Canada, France, China, Russia, and the United States. Its seeds harbor microbiota including bacteria and fungi, either on the surface (epiphytes) or within seed tissues (endophytes). These microbes can be pathogenic or beneficial, enhancing seedling metabolism and protection from environmental stresses. Characterizing beneficial seed microbiota enables development of microbial inoculants for seed treatment. Seeds from 12 varieties (green or yellow seed coats) were harvested from Sturgis and Dakota Lakes in 2021. Epiphytes were extracted using sonication followed by grinding the seeds to obtain endophytes. Isolates …

Enhancing Bone Regeneration Using Kaempferol As An Osteoprotective Compound: Signaling Mechanisms, Delivery Strategies, And Potential Applications, Mojdeh Salehi Namini, Sanam Mohandesnezhad, Sadaf Mohandesnezhad, Vahid Mansouri, Lobat Tayebi, Nima Beheshtizadeh

Electrical & Computer Engineering Faculty Publications

Various plants, including fruits, vegetables, and spices, contain kaempferol, a bioflavonoid compound with diverse medicinal effects, such as antioxidant, antibacterial, and anti-inflammatory characteristics. Furthermore, this compound exhibits multiple health-promoting properties, including osteoprotection and osteogenesis, primarily by modulating various cell-signaling pathways. This review aims to illustrate the medical advantages of kaempferol and its role in regulating bone metabolism through cell signaling mechanisms. Numerous studies have demonstrated the bone-protective properties of kaempferol and its encapsulated form. Further research is needed to clarify the optimal dosages, toxicity, safety, and other potential mechanisms of action. This review demonstrates that several signaling pathways, including nuclear …

Phylogenomics And Biogeography Of Zinniinae (Asteraceae: Heliantheae) Using Plastome And Nuclear Datasets, Samuel Victor Skibicki

Graduate Theses, Dissertations, and Problem Reports (ETD)

Zinniinae is a subtribe of plants in the daisy family (Asteraceae) and the sunflower tribe (Heliantheae). Traditionally, seven genera were included in the subtribe: Echinacea, Heliopsis, Philactis, Sanvitalia, Tehuana, Trichocoryne, and Zinnia. The subtribe is poorly understood from a phylogenetic and taxonomic standpoint, and I undertook a phylogenetic investigation of Zinniinae, using both the plastid genome (plastomes) and the nuclear genome, to learn more about the evolutionary history of this clade. Plastomes were assembled to build a phylogenetic tree, targeted sequence capture was used to compile the nuclear datasets, and a chronogram was created to estimate date ranges for …

Comparison Of Compositional Characteristics Of Protein Extracted From Spent Hemp Seed Cakes By One-Step Defatting With Organic Solvents Followed By Isoelectric Solubilization Precipitation Using Strong And Weak Acids, Miah Nicole Gathman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hemp seed cakes (HSC) are the byproduct of pressing hemp seeds for oil and are a significant agricultural waste product, with hundreds of tons produced annually. These nutrient-rich cakes have potential for sustainable applications in animal feed and food products. However, they could offer even greater value to the food industry through further extraction of the lipids and separation of the proteins they contain. Protein is a key nutritional component and companies are seeking cost-effective, time-efficient processes to concentrate protein from underutilized resources like HSC. Hexane (HEX) is a common solvent used in one-step defatting processes but may cause issue …

Evaluating The Adaptation Of Taro (Colocasia Esculenta) To West Virginia Climatic Conditions, Kayode Joseph Ajayi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Climate change impacts agriculture and food security across the globe, therefore higher temperatures could increase in the length of the growing period and new crops would take advantages of. Research was conducted in Morgantown, WV in 2022 and 2023 to understand the growth pattern of Taro in West Virginia, and to analyzing the chemical composition of the Taro plant. The experiment was a completely randomized design in direct planting-low tunnel technology (2 replications, and 10- plants/plot), and two fertilization treatments (fertilized and non-fertilized). Spacing was 1 m within and between rows. Data were subjected to ANOVA using SAS. Dependent variables …

Bridging Prairie Restoration And Regenerative Agriculture With Compost Tea / The Rocket Incident: Post-Fire Soil Microbe Recovery On The Eastern Washington University Restoration Site, April Hersey

EWU Masters Thesis Collection

Inland Pacific Northwest prairie is an endangered ecosystem that has been reduced by conversion to agriculture. There is growing interest in the role of soil microorganisms in prairie ecosystems, which may be crucial for restoration of the plant community through interspecies interactions within the rhizosphere. One method for improving soil microorganism community composition is compost tea: enriched liquid compost extract. Compost tea is used in regenerative agriculture to improve crop yields and protect against pathogens. While research has supported compost tea application in regenerative agriculture, it has not been widely studied in the context of ecosystem restoration. After beginning a …

Effects Of Gas Exchange Rates Between C3 And C4 Plant Species, Samantha L. Moya, Mollie Ouk, Aisha Goni

UROP Posters

Spinach and basil (C3 plants) were compared with corn (C4 plant) to

explore how photosynthetic pathways affect CO2 consumption. Corn

seeds were obtained and planted in Miracle-Gro® soil to be observed

over the duration of a month. Leaves (starting from 3,4, and 10) from

each plant were then taken and placed into a bottle and closed off

with a CO2 gas probe that measured levels of carbon dioxide in ppm.

Spinach and basil leaves were used as a comparison for measuring

the rate of CO2 concentration. This is done to compare the gas

exchange rates between C3 and C4 plants. …

Longitudinal And Ensemble Modeling Of Beef Cattle Feed And Water Intakes, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Beef cattle production remains a cornerstone of global food systems, providing highly bioavailable protein and essential micronutrients that are difficult to secure from plant-based foods alone. Yet this production is also resource-intensive, requiring substantial land, feed, and water inputs while contributing significantly to greenhouse gas emissions. Efficiency of dry matter intake (DMI) and water intake (WI) is central to both productivity and sustainability, but accurate measurement of these traits—particularly in grazing systems—remains a major limitation. National WI standards continue to rely on pen-level data collected in the 1950s, while most existing DMI prediction equations perform poorly when applied beyond the …

Microbial And Plant Biodiversity In Appalachian Agroecosystems And Their Implications For Soil Health, Kinsey Reed Close

Graduate Theses, Dissertations, and Problem Reports (ETD)

The sustainability of agricultural landscapes depends on understanding the complex interactions between plants, soil, and microbes. In Appalachia, a region shaped by complex terrain and long histories of human and ecological interaction, small family farms produce hay, livestock, and row crops. These agroecosystems, though often treated as “artificial” by many ecologists, have the potential to support both food production and biodiversity conservation if managed with an understanding of ecological complexity. This dissertation examines how plant and microbial diversity interact with soil and management to influence nutrient cycling, soil health, and long-term resilience in Appalachian agricultural systems. A literature review revealed …

Determination Of Manganese Concentration In Forages, Soil, Sewage Water Of Three Suburban Areas Of Sargodha, Pakistan, Iqra Anwar, Zafar Iqbal Khan, Kafeel Ahmad, Naila Riaz, Faiza Zubair, Asma Ashfaq, Muhammad Fahad Ullah, Farzana Siddique, Ijaz Rasool Noorka, Abid Ejaz, Maloofa Manzoor, Umbreen Bibi, Hafiza Memona Nazir, Saif Ullah

Journal of Bioresource Management

For irrigation of soil utilization of wastewater has risen significantly in the past few years. This wastewater often have large amount of trace elements and heavy metals, many of which are non-essential and harmful to living organisms such as plants, animals, and humans. In Pakistan, particularly around suburban areas, it is common to use this polluted water for growing vegetables. When such water is used for irrigation over an extended period of time, toxic substances can accumulate in the soil, potentially causing toxicity to plants and degrading soil quality. The samples analyzed in the study were prepared using the wet …

Biphasic Models Improved S-Metolachlor Dissipation Endpoint Predictions, K. Badou-Jeremie Kouame, Mary Savin, Cammy Willett, Thomas R. Butts, Erin Grantz, Matthew Bertucci, Samantha E. Robinson, Rodrigo Werle, Nilda Roma-Burgos

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Accurate predictions of herbicide dissipation endpoints are essential for making environmental management decisions, estimating the duration of weed control, and deciding crop replant and rotation options. The performance of the single first-order (SFO), first-order multiple-compartment (FOMC), bi-exponential (BEXP), first-order double-exponential decay (FODED), and first-order two-compartment (FOTC) was evaluated to describe S-metolachlor dissipation. Results showed that the BEXP and FOTC were not appropriate. The FODED was ranked as the top model with the lowest Akaike's information criterion (AICc) value among the other three candidate models and was followed by the FOMC model. The SFO displayed the largest root mean square …

Function Over Form: The Benefits Of Aspen As Surrogate Brood-Rearing Habitat For Greater Sage-Grouse, Michel T. Kohl, Charles P. Sandford, Paul C. Rogers, Renee Chi, Terry A. Messmer, David K. Dahlgren

Aspen Bibliography

Species of conservation concern are often habitat specialists, posing significant risk to those species when specific plant communities are threatened. As a result, practitioners habitually focus conservation efforts on these communities while ignoring ecological mechanisms that explain the wildlife–plant relationships. In doing so, practitioners may overlook alternative vegetation communities that could maintain wildlife populations under alternative conditions (e.g., climate change). Here, we term these areas surrogate habitat, defined as vegetation communities or resource sites that provide similar critical resources as conventional sites, and assess their potential for conservation using a case study of greater sage-grouse (Centrocercus urophasianus) on …

Evaluating The Effectiveness Of Benguet Pine (Pinus Kesiya) Xylem For Deep Well Water Filtration, Allen Conner C. Hizon, Robert Zachary M. Santos, Juan Jose B. Lacson, Jefferson Danilo G. Villarin, Juan Carlo T. Gumangan

Sinaya: A Philippine Journal for Senior High School Teachers and Students

Even today, many rural and remote communities still do not have access to safe drinking water, which is a basic right of every human. To address this problem, the effectiveness of the Benguet pine tree (Pinus kesiya) xylem as a sustainable water filtration method for well water was investigated. The physical and chemical properties of the filtered and unfiltered deep well water were tested: the total dissolved solids (TDS) and the electrical conductivity using a 5-in-1 electronic water quality tester and the pH level using pH strips. On the other hand, the microbiological metrics, such as heterotrophic plate …

Re-Indigenizing And Collaboratively Tending Urban Greenspaces, Divinity Nnenne Adewale

University Honors Theses

This work details the active process of land tending with the intention of re-Indigenization in the Pacific Northwest at several Portland sites; The Native American Student Community Center, PSU Oak Savanna, and the Ridgefield National Wildlife Refuge. Land tending is an intuitive process, one rooted in love and respect for life of all forms as well as becoming the perpetual student; land tending is not a skill to be mastered, only practiced. Unlike environmental management, tending cannot be done separate from the earth, in a classroom or otherwise. Connections are what tending is built on top of: the foundation; effort …

Tree Rings Reveal Mixtures Of Aspen And Spruce Exhibit Greater Drought Resilience In A Planted Field Experiment, Jéssica Chaves Cardoso, Linhao Wu, Marcel Schneider, Charles A. Nock

Aspen Bibliography

Boreal forests provide a wealth of ecosystem services, many of which are linked to forest productivity. Climate change is expected to increase the frequency, duration, and severity of drought, and undermine the productivity of western boreal forests. Favouring species mixtures has emerged as a potential strategy to increase forest resilience to drought. To test the hypothesis that mixtures of trembling aspen and white spruce were more productive than pure stands because they were more resistant and resilient to drought, we analyzed community and species responses to drought utilizing measurements of annual growth obtained from stem disc samples from a planted …

Sensitivity Of Difficult-To-Control Palmer Amaranth Accessions To Fluridone And Diflufenican, Maria Carolina Souza, Matthew Coleman Woolard, Jason K. Norsworthy, Tristen H. Avent, Tristen H. Avent, Tom Barber, Rodrigo Botelho

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Palmer amaranth (Amaranthus palmeri S. Watson) has evolved resistance to herbicides targeting nine sites of action. Phytoene desaturase-inhibiting herbicides remain among the few sites of action that are still effective in controlling Palmer amaranth. Greenhouse experiments were conducted to evaluate the effectiveness of fluridone and diflufenican in controlling difficult-to-control Palmer amaranth accessions collected in Arkansas from 2016 to 2022, to quantify the response of three accessions that exhibited low sensitivity to both herbicides, and to evaluate the effectiveness of photosystem II inhibitors alone and with fluridone or diflufenican on three accessions. At 14 days after a preemergence application, Palmer …

Investigation And Computational Analysis Of The Iqd Gene Family In Common Bean (Phaseolus Vulgaris L.): Expression Profiling Under Salt Stress, Tarik Kirlioğlu, Aybüke Okay, Emi̇ne Sümer Aras, İlker Büyük

Turkish Journal of Botany

Common bean is one of the most nutritionally important legumes because of its high-quality protein content. While its genome has been widely studied however, the IQD gene family has not been extensively investigated in common bean to date. The IQD proteins (IQ-67 domain), a type of calmodulin-binding protein, are involved in plant growth and development, as well as the plant's response to environmental stressors. In this study, thirty-six PvIQD genes were discovered and their proteins were found to be classified into four groups according to the phylogenetic tree. The proteins which are encoded by PvIQD genes with similar exon-intron pattern …

Primary Distribution And Rt-Pcr-Based Detection Of Citrus Dwarfing Viroid (Cdvd) In Citrus Cultivars Of Pakistan, Zia Ul Haq, Amjad Ali, Syed A.H. Naqvi, Faheem Shehzad Baloch, Ummad U.D. Umar

Turkish Journal of Botany

Viroid diseases have emerged as a significant threat to citrus orchards globally. In Pakistan, viroid infections have rapidly spread across different citrus cultivar, posing serious challenges to the survival of orchards. This study encompasses the determination of the prevalence and detection of Citrus dwarfing viroid (CDVd) in both reported and newly introduced citrus cultivars in Pakistan using RT-PCR. RNA was extracted from 122 infected citrus samples and examined for the presence of viroids following the successful optimization of newly designed CDVd primers (CDVd AF1/AR1). This led to the confirmation of CDVd in 72 samples (59.01%).. Biological indexing was performed using …

Functional Characterization Of Haloarchaeal Udp-Glucose 4-Epimerase And 3-Hydroxybutyryl-Coa Dehydrogenase Genes In Nicotiana Tabacum, Çi̇ğdem Otur, Sezer Okay, Aslihan Kurt Kizildoğan

Turkish Journal of Botany

The objective of the studyAbiotic stress poses a significant threat to plant survival. In response to the challenging conditions, plants have evolved various resistance mechanisms. Recently, genetic strategies have become increasingly prevalent in efforts to enhance abiotic stress tolerance and develop stress-resistant plant varieties. In this study, we employed heterologous expression of two metabolism-related haloarchaeal genes in the Nicotiana tabacum genome to improve its mechanism for salt and drought tolerance.Experimental procedure(s)The U2642 and CL165 genes encoding 3-hydroxybutyryl-CoA dehydrogenase and UDP-glucose 4-epimerase, respectively, were subcloned into the pIPKb004 vector under the control of Cauliflower mosaic virus 35S (CaMV35S) promoter using the …

Genome-Wide Identification And Characterization Of The Lea Gene Family In Common Bean And The Role Of The Dehydrin Gene Pvhdn3 In Enhancing Salt And Drought Tolerance In Transgenic Tobacco Plant., Gökhan Gökdemi̇r, Zafer Seçgin, Karam Mostafa, Mohamed Farah Abdulla, Musa Kavas

Turkish Journal of Botany

Global climate change exposes crops to numerous abiotic and biotic stress factors, significantly threatening their growth, development, and overall productivity. Among the various abiotic stresses, salt and drought are the most detrimental, having a severe impact on yield stability. Late Embryogenesis Abundant proteins (LEA) represent a broad and varied class of polypeptides that accumulate in response to cellular dehydration across diverse organisms. Nonetheless, there has been a scarcity of studies on this protein family in common beans prior to this investigation. In this study, we identified 69 members of the LEA gene family in common bean, characterized by conserved LEA …

Phylogenomics Of The Tribe Leonureae Reveals A New Genus: Paraleonurus (Lamiaceae, Lamioideae), Mei-Lin Qian, Ya-Ping Chen, Tuncay Di̇rmenci̇, Yue Zhao, Guo-Xiong Hu, Fei Zhao, Ferhat Celep, Bo Li, Chunlei Xiang

Turkish Journal of Botany

With approximately 80 species across six genera (Leonurus, Lagopsis, Panzerina, Lagochilus, Chaiturus, and Loxocalyx), the tribe Leonureae represents one of the most morphologically diverse tribes within the subfamily Lamioideae of Lamiaceae. Previous studies indicated that Leonurus and Lagopsis are not monophyletic, and relationships among and within these genera remain unclear due to limited sampling and DNA markers. In this study, we present the most comprehensive phylogenomic analyses of Leonureae based on complete plastome sequences and 127 nuclear loci retrieved from next-generation sequencing data. Our results provide strong evidence for the monophyly of Leonureae, which can be further divided into six …

Heterologous Expression Of Haloarchaeal Transporter Genes In Nicotiana Tabacum Under Salt And Drought Stresses, Çi̇ğdem Otur, Musa Kavas, Aslihan Kurt Kizildoğan

Turkish Journal of Botany

The objective of the study: Climate change and human activities impose severe biotic and abiotic stresses that threaten plant survival. To cope with these extreme conditions, plants have evolved various resistance mechanisms. In recent years, genetic approaches have been increasingly employed to enhance abiotic stress tolerance and develop stress-resistant plants. In this study, we introduced three transporter-related genes from extreme haloarchaea, which thrive in saturated NaCl environments, into the Nicotiana tabacum genome to improve its salt and drought tolerance mechanisms.E xperimental procedure(s) : The U2845, U3508, and CL165 genes were subcloned into the pIPKb004 vector, which includes the cauliflower mosaic …

Innovations In Plant Genome Editing And Biotechnology: Addressing Global Agricultural Challenges, Bayram Ali̇ Yerli̇kaya, Karam Mostafa, Abdullah Aydin, Seher Yerli̇kaya, Momna Mehmood, Ni̇sa Nur Yilmaz, Kubi̇lay Yildirim, Musa Kavas

Turkish Journal of Botany

Developments in genome editing and plant biotechnology in recent years, especially CRISPR-Cas9 and RNA interference (RNAi) technologies, offer essential tools for solving global agricultural problems that are worsened by climate change and the increasing food demand caused by the rising world population. This review focuses on the urgent need to feed a growing global population. It explores how key biotechnological tools can enhance crop resilience and nutritional quality amid climate variability and extreme weather. The applicability of CRISPR-Cas technologies in the development of climate-friendly, more nutritious, and higher-yielding crops has opened a biotechnological avenue for the development of both transgenic …

Advancements In Qtl Mapping And Gwas Application In Plant Improvement, Muhammad Tanveer Altaf, Muhammed Tatar, Amjad Ali, Waqas Liaqat, Parnaz Mortazvi, Ceyhun Kayihan, Fati̇h Ölmez, Muhammad Azhar Nadeem, Jazib Javed, Jin-Ying Gou, Meng-Lu Wang, Ummad Ud Din Umar, Hayri̇ye Yildiz Daşgan, Cemal Kurt, Mehtap Yildiz, Sheikh Mansoor, Abdelfattah A. Dababat, Nafiz Çeli̇ktaş, Faheem Shehzad Baloch

Turkish Journal of Botany

Molecular markers play a crucial role in modern plant breeding by enabling the precise identification of genetic loci associated with important agronomic traits. This article focuses on the advancements in Quantitative Trait Locus (QTL) mapping and Genome-Wide Association Studies (GWAS), which are pivotal in identifying and dissecting complex traits such as stress tolerance, yield improvement, and nutrient efficiency. Through QTL mapping, significant genetic regions linked to desired traits are identified, while GWAS leverages larger populations for higher resolution in genetic association studies. The integration of next-generation sequencing, CRISPR, and transcriptomics technologies has revolutionized the precision and efficiency of these methods, …