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Summaries Of Arkansas Cotton Research 2007, Derrick M. Oosterhuis

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, July 1 — September 30, 2008, Margaret N. Rees

Fire Science

• Cones monitored and reseeded to obtain plants for nursery study.

• Goodsprings revegetation studies monitored.

• Website developed for JFS study.

• Writing short communication for Journal of Arid Environments.

• Working on poster for NAA conference in October.

Lake Mead National Recreation Area Vegetation Monitoring And Analysis: Year-End Progress Report, October 1, 2007 To September 30, 2008, Margaret N. Rees

Vegetation Monitoring

Executive Summary

• The Weed Sentry program surveyed over 335 miles and 2,610 acres of transportation corridors on federal land in Clark County for incipient populations of exotic plants.
• 18 manuscripts attained in-press or published status and one manuscript was submitted for review this year. Among published manuscripts, are one documenting Sahara mustard germination patterns (Western North American Naturalist), one assessing exotic species distributions in Clark County (Environmental Monitoring and Assessment), and another is a synthesis of burro effects on Mojave Desert vegetation (Environmental Management), and one assessing vegetation in grassy remnants of the Las Vegas Valley (Desert Plants)
• Staff delivered …

The Very-Long-Chain Hydroxy Fatty Acyl-Coa Dehydratase Pasticcino2 Is Essential And Limiting For Plant Development, Liên Bach, Louise V. Michaelson, Richard Haslam, Yannick Bellec, Lionel Gissot, Jessica Marion, Marco Da Costa, Jean-Pierre Boutin, Martine Miquel, Frédérique Tellier, Frederic Domergue, Jennifer E. Markham, Frederic Beaudoin, Johnathan A. Napier, Jean-Denis Faure

Department of Biochemistry: Faculty Publications

Very-long-chain fatty acids (VLCFAs) are synthesized as acyl-CoAs by the endoplasmic reticulum-localized elongase multiprotein complex. Two Arabidopsis genes are putative homologues of the recently identified yeast 3-hydroxy-acyl-CoA dehydratase (PHS1), the third enzyme of the elongase complex. We showed that Arabidopsis PASTICCINO2 (PAS2) was able to restore phs1 cytokinesis defects and sphingolipid long chain base overaccumulation. Conversely, the expression of PHS1 was able to complement the developmental defects and the accumulation of long chain bases of the pas2–1 mutant. The pas2–1 mutant was characterized by a general reduction of VLCFA pools in seed storage triacylglycerols, cuticular waxes, …

Inhibitors Of Plant Peptide Deformylase For Use As Broad-Spectrum Herbicides And Methods For Identifying The Same, Robert L. Houtz, Lynnette M. A. Dirk, Mark Alan Wiliams

Horticulture Faculty Patents

The invention relates to a method of identifying herbicides and to the use of inhibitors of plant peptide deformylase as broad spectrum herbicides.

The Impact Of Outcrossing On Yields Of ‘Hass’ Avocado, Lauren C. Garner, Vanessa E.T.M. Ashworth, Michael T. Clegg, Carol J. Lovatt

Horticulture and Crop Science

‘Hass’ avocado (Persea americana Mill.) is characterized by excessive flower and fruit abscission, resulting in extremely low fruit set. Low outcrossing rates might be a factor contributing to low yields. It is hypothesized that self-fertilized flowers and resulting fruit abscise at a much higher rate than fruit that are the product of outcrossing. However, significant relationships between outcrossing rates and yields have only been established in a few avocado studies. The objective of this research was to investigate the importance of outcrossing to yield in a commercial ‘Hass’ orchard containing ‘Bacon’, an effective pollinizer of ‘Hass’. Microsatellite markers were used …

Shrub-Interspace Dynamics Alter Relationships Between Microbial Community Composition And Belowground Ecosystem Characteristics, Rebecca E. Drenovsky, Z. T. Aanderud, M. I. Shuldman, J. H. Richards

Biology

In desert ecosystems, belowground characteristics are influenced chiefly by the formation and persistence of “shrub-islands of fertility” in contrast to barren plant interspaces. If soil microbial communities are exclusively compared between these two biogeochemically distinct soil types, the impact of characteristics altered by shrub species, especially soil C and N, are likely to be overemphasized and overshadow the role of other characteristics in structuring microbial composition. To determine how belowground characteristics influence microbial community composition, and if the relative importance of these characteristics shifts across the landscape (i.e., between and within shrub and interspace soils), changes in microbial communities across …

A Case Study Of A Successful Lake Rehabilitation Project In South-Central Nebraska, Peter J. Spirk, Brad Newcomb, Keith D. Koupal

The Prairie Naturalist

Cottonmill Lake, a 17.4 ha impoundment located in Buffalo County, Nebraska, was a fishery dominated by common carp (Cyprinus carpio). As a result of the poor sportfish populations, angler participation in May and June of 1993 was low (503 ± 210 angler hours) and angler catch rates for all fish species (0.5 ± 0.4 fish/angler hour) was less than desired. In 1995, before rehabilitation, bluegill (Lepomis macrochirus) trap net catch per unit effort (CPUE) was 1.5 ± 0.9, largemouth bass (Micropterus salmoides) catch per hour of electrofishing was 8.0 ± 0.5, and channel catfish (Ictalurus punctatus) …

The Prairie Naturalist. Volume 40, No. 3/4 September/December 2008, The Great Plains Natural Science Society

The Prairie Naturalist

WINTERKILL AND BIOMASS OF THE PAINTED TURTLE IN A SOUTH DAKOTA WETLAND. S. G. Platt, Z. Fast Horse, W. Cross, S. Mannel, and T. R. Rainwater

NON-BLACKBIRD AVIAN OCCURRENCE AND ABUNDANCE IN NORTH DAKOTA SUNFLOWER FIELDS. O. A. Schaaf, G. M. Linz, C. Ooetkott, M. W. Lutman, and W. J. Bleier

USING LOCAL KNOWLEDGE AND REMOTE SENSING TO MAP KNOWN AND POTENTIAL PRAIRIE-CHICKEN DISTRIBUTION IN KANSAS. M. E. Houts, R. O. Rodgers, R. D. Applegate, and W. H. Busby

A CASE STUDY OF A SUCCESSFUL LAKE REHABILITATION PROJECT IN SOUTH-CENTRAL NEBRASKA. P. J. Spirk, B. A. Newcomb, and K. O. …

Non-Blackbird Avian Occurrence And Abundance In North Dakota Sunflower Fields, Dionn A. Schaaf, George M. Linz, Curt Doetkott, Mark W. Lutman, William J. Bleier

The Prairie Naturalist

Sunflower fields are well-documented as foraging habitat for fallmigrating blackbirds (Family Icteridae). There is, however, a paucity of information on the use of sunflower fields by non-blackbirds. We assessed non-blackbird use of 12 ripening sunflower fields in the Prairie Pothole Region of central North Dakota. From mid-August to mid-October 2000, we counted 4,129 individual birds, consisting of 22 families and 61 species, in the sample fields and within 5 m of the field edges. We saw the largest number of birds from 18 September to 27 September. The Family Emberizidae (sparrows) accounted for 26% of the species and 20% of …

Winterkill And Biomass Of The Painted Turtle In A South Dakota Wetland, Steven G. Platt, Zannita Fast Horse, Warren Cross, Sylvio Mannel, Thomas R. Rainwater

The Prairie Naturalist

Winterkill occurs when drought conditions expose hibernating turtles to desiccation and lethaly cold temperatures. Winterkill is thought to represent a major source of mortality in northern populations of the painted turtle (Chrysemys picta), but few field observations are available. We herein reported on catastrophic winterkill among western painted turtle (C. pieta bellii) at Limestone Butte Lake (LBL) in western South Dakota during the winter of 2003-2004. Additionally, we used the carcasses of winterkilled turtles (n = 86) to estimate the standing crop biomass of the painted turtle at LBL (0.6 kg/ha). This was the only estimate …

Using Local Knowledge And Remote Sensing To Map Known And Potential Prairie-Chicken Distribution In Kansas, Michael E. Houts, Randy D. Rodgers, Roger D. Applegate, William H. Busby

The Prairie Naturalist

The greater prairie-chicken (Tympanuchus cupido) and lesser prairie-chicken (Tympanuchus pallidicinctus) have experienced considerable fluctuations in their range and distribution over time. Having current range maps would help wildlife managers and policy makers with decisions regarding prairie-chicken habitat. To create an updated and accurate map of the Kansas prairie-chicken range, a two-pronged approach was implemented. First, a map of potential habitat was created by using known habitat preferences and avoidance factors. Second, a preliminary map showing the distribution of greater and lesser prairie-chickens was created and mailed to regional experts for comments and edits. The returned edits …

Epigenetic Transcriptional Repression Of Cellular Genes By A Viral Set Protein, Shiraz Mujtaba, Karishma L. Manzur, James Gurnon, Ming Kang, James L. Van Etten, Ming-Ming Zhou

Department of Plant Pathology: Faculty Publications

Viruses recruit host proteins to secure viral genome maintenance and replication. However, whether they modify host histones directly to interfere with chromatin-based transcription is unknown. Here we report that Paramecium bursaria chlorella virus 1 (PBCV-1) encodes a functional SET domain histone Lys methyltransferase (HKMTase) termed vSET, which is linked to rapid inhibition of host transcription after viral infection. We show that vSET is packaged in the PBCV-1 virion, and that it contains a nuclear localization signal and probably represses host transcription by methylating histone H3 at Lys 27 (H3K27), a modification known to trigger gene silencing in eukaryotes. We also …

Estimating Soil Seed Bank Characteristics In Ponderosa Pine Forests Using Vegetation And Forest-Floor Data, Scott R. Abella, Judith D. Springer

Public Policy and Leadership Faculty Research

Soil seed banks are important for vegetation management because they contain propagules of species that may be considered desirable or undesirable for site colonization after management and disturbance events. Knowledge of seed bank size and composition before planning management activities facilitates proactive management by providing early alerts of exotic species presence and of abilities of seed banks to promote colonization by desirable species. We developed models in ponderosa pine (Pinus ponderosa) forests in northern Arizona to estimate the size and richness of mineral soil seed banks using readily observable vegetation and forestfloor characteristics. Regression models using three or fewer predictors …

Nest Site Preference And Nesting Success Of Upland Sandpiper On Grazing Systems In East Central North Dakota-, Blane A. Klemek

The Prairie Naturalist

require ranchers to move their cattle throughout the range from one paddock to another, thereby allowing formerly grazed paddocks to regenerate. This is beneficial in a number of ways: overgrazing is reduced, cattle weight-gains are improved, suitable nesting cover for ground nesting birds is produced, and the overall health of the grassland is enhanced (Sedivec and Barker 1991). The objectives of my study were to investigate nesting success of the upland sandpiper as a function of grazing regime. Specifically, I was interested in comparing nesting success between RGS and non-rotational grazing systems (NRGS) and to examine relationships between nest site …

Semi-Melanistic White-Tailed Deer In Northern Wisconsin, Christopher N. Jacques, Keith R. Mccaffery, Jonathan Jenks, John T. Baccus

The Prairie Naturalist

Melanistic color morphs of white-tailed deer (Odocoileus virginianus) are differentiated from other recognized color morphs by having uniform black hairs on the dorsal surface with subdued black hairs on the ventral surface, dark face and ears, a distinctive mid-dorsal stripe extending from the head to the apex of the tail, and a tail with black dorsally and white ventrally (Baccus and Posey 1999). Melanism results from the overproduction of the skin pigment melanin and is considered rare in white-tailed deer populations (Severinghaus and Cheatum 1956, Sauer 1984, Smith et al. 1984). Semi-melanistic deer have the same dark pelage …

The Prairie Naturalist 2008, Volume 40: Reviewers, Author Index, & Subject Index

The Prairie Naturalist

No abstract provided.

Intsormil Scientists Develop Insect Resistant Sorghum And Pearl Millet For Africa And The Usa, Intsormil

INTSORMIL Impacts and Bulletins

The aphids Melanaphis sacchari (sugarcane aphid) in Africa and Schizaphis graminum (greenbug, see above) in the United States; panicle bugs; sorghum midge, Stenodiplosis sorghicola; stalk borers and termites infest and reduce yields of sorghum. The millet head miner, Heliocheilus albipunctella, reduces grain yield and quality of pearl millet.

INTSORMIL entomologist Bonnie Pendleton, West Texas A&M University, collaborates with African scientists to develop management strategies for sorghum and millet insects. Emphasis is on developing plants resistant to pests. Hundreds of sorghum and pearl millet genotypes have been evaluated by INTSORMIL scientists for grain yield and quality and resistance to …

Cover Page

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

Alternation Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Steven W. Bowen, Balazs Siminszky, Lily Gavilano

Plant and Soil Sciences Faculty Patents

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) inNicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in …

The Leaf Ionome As A Multivariable System To Detect A Plant's Physiological Status, Ivan R. Baxter, Olga Vitek, Brett Lahner, Balasubramaniam Muthukumar, Monica Borghi, Joe Morrissey, Mary Lou Guerinot, David E. Salt

Dartmouth Scholarship

The contention that quantitative profiles of biomolecules contain information about the physiological state of the organism has motivated a variety of high-throughput molecular profiling experiments. However, unbiased discovery and validation of biomolecular signatures from these experiments remains a challenge. Here we show that the Arabidopsis thaliana (Arabidopsis) leaf ionome, or elemental composition, contains such signatures, and we establish statistical models that connect these multivariable signatures to defined physiological responses, such as iron (Fe) and phosphorus (P) homeostasis. Iron is essential for plant growth and development, but potentially toxic at elevated levels. Because of this, shoot Fe concentrations are …

Impacts Of Root Invigoration And Its Individual Components On The Performance Of Red Maple (Acer Rubrum), Kelby Fite

All Dissertations

The Root Invigoration process involves soil decompaction with an air tool, amendment with organic matter and prescription fertilizer, and mulching. In the current study, we measured soil chemical and physical properties, tree characteristics, and root system responses to this process and its individual components. Treatments included Root Invigoration (AFM), mulch only (M), fertilization only (F), Airspade® tillage only (A), and an untreated control (C). The experiment was conducted from 2005-2007 at four urban sites: Anderson, SC; Boston, MA; Myrtle Beach, SC and Pittsburgh, PA. Soil strength was initially reduced by Airspade®, mulch and AFM; however only AFM-treated soils sustained this …

Characterization Of The Pathway Leading To The Synthesis Of Salicylic Acid In Plants Resisting Pathogen Infection., Alexander Eddo

Electronic Theses and Dissertations

Salicylic acid is a plant hormone that accumulates with plant-pathogen interaction. This accumulation corresponds to the plant being resistant to infection and without it the plant is susceptible. In this study, primers of genes involved in the normal synthesis of SA were used in RT-PCR to compare gene expression levels in susceptible and resistant plants challenged with tobacco mosaic virus. Because SA synthesis shares chorismate as a common substrate with the synthesis of aromatic amino acids, HPLC was used to determine whether the increase in SA could be attributed to a decrease in amino acid levels. The results suggest that …

Synthesis Of Novel Agrochemicals As Potential Plant Immunization Agents., Arrey Besong Enyong

Electronic Theses and Dissertations

The world's population is expected to grow from 6 billion to about 10 billion by 2050. The greatest population increase is expected to occur in Africa, Latin America, and Asia. To feed a world with huge increases in population and to sustain the well-being of humans, a large increase in food production must be achieved. The projected increase in food production must be accomplished on the existing cultivated areas because the expansion of new land is limited by environmental concerns, urbanization and increasing water scarcity.

Different compounds have been developed for the "immunization" of plants against several pathogens. These compounds …

Gender Variation In The Prezygotic Reproductive Effort Of The Common Silver Moss, Kimberley Horsley, Lloyd Stark

Undergraduate Research Opportunities Program (UROP)

The bryophyte Bryum argenteum is a vigorous moss with a cosmopolitan distribution and high tolerance to desiccation and temperature stress. Due to its widespread nature, B. argenteum has been highly investigated (e.g., Chopra and Bhatla 1981). However, very little research has been done on the reproductive aspects of this moss with respect to gender differences. This project will address the prefertilization reproductive efforts of the moss, and look for any differences in the rate of growth and sexual expression between the sexes. The hypothesis to be tested is that males actually have a higher prefertilization reproductive effort than females and …

Decoding The Protein Interaction Network - An Approach Integrating Biology And Math, Ryan Huang, Lingkun Gu, Qingxi J. Shen

Undergraduate Research Opportunities Program (UROP)

The WRKY super family is known to play a major role during the plant stress response and development. My project focuses on the protein-protein interaction of an Oryzasativa (rice) transcription factor, OsWRKY71 which functions as the repressor of gibberellins signaling pathway. Previous literature revealed that OsWRKY71 can interact with itself or OsWRKY51 to form dimmers by using bimolecular fluorescence complementation (BiFC). To confirm this result, we use yeast two-hybrid system. As our data showed, OsWRKY71 seems to suppress the reporter gene expression of the conventional yeast two-hybrid system, so we use a modified yeast two-hybrid, Mating-based Split Ubiquitin System (MbSUS). …

Relative Crystallinity Of Plant Biomass: Studies On Assembly, Adaptation And Acclimation, Darby Harris, Seth Debolt

Horticulture Faculty Publications

Plant biomechanical design is central to cell shape, morphogenesis, reproductive performance and protection against environmental and mechanical stress. The cell wall forms the central load bearing support structure for plant design, yet a mechanistic understanding of its synthesis is incomplete. A key tool for studying the structure of cellulose polymorphs has been x-ray diffraction and fourier transform infrared spectroscopy (FTIR). Relative crystallinity index (RCI) is based on the x-ray diffraction characteristics of two signature peaks and we used this technique to probe plant assembly, adaptation and acclimation. Confocal microscopy was used to visualize the dynamics of cellulose synthase in transgenic …

Genetic Diversity In Native And Invasive Rubus (Rosaceae), Ashley A. Wint

Masters Theses & Specialist Projects

Invasive species are an increasing threat to biological diversity as well as a leading cause of recent species’ extinctions. Invasives spread quickly and efficiently, and the U.S spends millions of dollars annually in the control and eradication of these species. More information is necessary in order to predict which species may become invasive. Rubus (Rosaceae) was chosen for study because this genus includes various ploidy levels, reproductive modes, and species that are invasive as well as native.

Three Rubus species were chosen to represent apomictic and tetraploid invasives (Rubus armeniacus), a sexual and diploid native species (R. occidentalis), and a …

Phytopathogen Type Iii Effector Weaponry And Their Plant Targets, Anna Block, Guangyong Li, Zheng Qing Fu, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

Phytopathogenic bacteria suppress plant innate immunity and promote pathogenesis by injecting proteins called type III effectors into plant cells using a type III protein secretion system. These type III effectors use at least three major strategies to alter host responses. One strategy is to alter host protein turnover, either by direct cleavage or by modulating ubiquitination and targeting to the 26S proteasome. Another strategy involves alteration of RNA metabolism by transcriptional activation or ADP-ribosylation of RNA-binding proteins. A third major strategy is to inhibit the kinases involved in plant defence signalling, either by removing phosphates or by direct inhibition. The …

Conversion Of Traditional Observation-Based Botany Labs To Investigative Inquiry Learning, Hajara Mahmood

Masters Theses & Specialist Projects

“Tell me and I forget, show me and I remember, involve me and I understand.” - Chinese Proverb. Involvement in learning implies possessing skills and attitudes that permit students to seek resolutions to questions and issues while constructing new knowledge.

Low enrollment in Plant Biology and Diversity and upper level plant science courses has been noticed at Western Kentucky University. In addition, graduating students performed below the national average on the senior assessment examination in the area of botany content knowledge offered by WKU’s Biology Department. This may be due to the fact that observation-based botany has been taught in …