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Heirloom And Hybrid Tomato Yield And Quality In Organic And Conventional Production Systems, Diana J. Edlin

Masters Theses & Specialist Projects

Due to the recent changes in the economy of Kentucky tobacco production, some producers are seeking an alternative crop that will provide similar economic gains to tobacco without needing more acreage. Tomatoes are an existing crop in Kentucky that have been declining in acreage over the last five years. There is evidence to suggest that, through niche and local marketing, tomatoes may be able to fill the void left by tobacco. However, there is concern among producers that they will lose yield and/or quality if they switch to one of these niche production systems or cultivars.

A two year study …

Detection And Measurement Of Water Stress In Vegetation Using Visible Spectrum Reflectance, Arthur Zygielbaum

Department of Geography: Dissertations, Theses, and Student Research

At any scale, from a single microbe to the planet that nurtures us, water defines our place in the universe. It provides the hydraulic forces needed to give plants structure, and the medium enabling photosynthesis, the basis for most life on Earth, to occur. Knowledge of plant water status is vital to understanding the state or condition of vegetation, information which is essential to disciplines as diverse as agriculture, geography, and climatology. Non-destructive and remote sensing of plant water status allows the gathering of such information across wide geographic extents and over long periods of time. Monitoring vegetation remotely requires …

Temperature Compensation Of Auxin Dependent Developmental Patterning, Nicholas J. Kaplinsky

Biology Faculty Works

The establishment of localized auxin gradients plays a central role in developmental patterning in plants. Auxin levels and responses have been shown to increase with temperature although developmental patterning is not affected. This suggests the existence of a homeostatic mechanism that ensures that patterning occurs normally over a range of temperatures. We recently described the cloning and characterization of BOBBER1 (BOB1), an Arabidopsis gene which encodes a small heat shock protein. BOB1 is required for the establishment of auxin gradients and for normal developmental patterning. BOB1 is also required for organismal thermotolerance and localizes to heat shock granules at elevated …

Patterns Of Bison Hair Use In Nests Of Tallgrass Prairie Birds, Bryan R. Coppedge

The Prairie Naturalist

I examined patterns of bison (Bison bison L.) hair use by passerine birds nesting in the Tallgrass Prairie Preserve, Osage County, Oklahoma, 2002-2004. I collected and dissected 103 nests of 15 species into their constituent components. Nests were predominately composed of herbaceous material such as grass stems and leaves. Woody material and mud were rarely used. Bison hair was the most prominent zoological material used in nests, with lesser amounts and occurrence of arthropod silk, snake skin, feathers, jack rabbit (Lepus californicus Oray) fur, and man-made materials such as cellophane and string. At least one nest of 13 …

Long Distance Molt Migration By A Giant Canada Goose From Eastern South Dakota, Bobby J. Anderson, Charles D. Dieter

The Prairie Naturalist

To reduce crop damage by resident giant Canada geese (Schaible et al. 2005), the South Dakota Department of Game, Fish and Parks (SDGFP) initiated a program to reduce goose nesting success in eastern South Dakota. One management tool used by SDGFP personnel was the destruction of giant Canada goose nests. When a giant Canada goose has its nest destroyed, they are known to initiate a molt migration (Mykut 2002, Luukkonen et aI. 2008). We attached Platform Transmitting Terminals (PTT; model ST -19) to document and describe molt migrations of giant Canada geese following nest destruction. We captured 3 adult nesting …

Noteworthy Winter Prey Of Short-Eared Owls In Southern Texas: A Case Study, Damon Williford, Marc C. Woodin, Mary Kay Skoruppa

The Prairie Naturalist

The winter range of North American short-eared owls (Asio flammeus) encompasses much of the United States, including southern Texas, where it is a common winter resident (Oberholser 1974, Rappole and Blacklock 1985). Winter food habits of short-eared owls are relatively weJl documented, but the majority of investigations have been conducted in eastern Canada and northeastern U.S. (Clark 1975, Holt 1993); midwestern U.S. (Colvin and Spaulding 1983); and British Columbia and Pacific northwestern U.S. (Bogiatto et al. 2001). The short-eared owl has a narrow trophic niche, generally preying on small mammals, with voles (Microtus spp.) and deer mice …

Monitoring Meadows With A Modified Robel Pole In The Northern Black Hills, South Dakota, Daniel W, Uresk, Daryl E. Mergen, Ted A. Benzon

The Prairie Naturalist

We used a modified Robel pole to measure vegetation for a study conducted in the northern Black Hills, South Dakota. Objectives were to determine the relationship between visual obstruction readings and clipped standing herbage, and develop guidelines for monitoring standing herbage. The relationship between visual obstruction readings and standing herbage was linear and regression coefficients were significant (P≤ 0.001). Herbage ranged from 140 to 3313 kg· ha-1 with a mean of 1386 kg· ha^-1 (SE = 320 kg· ha^-1) for 123 transects. Visual obstruction readings (VOR) ranged from 0.6 to 30.4 (number of 1.27 cm bands …

A Partial Inventory Of Islands In North Dakota: Potential For Breeding Waterfowl Management, Michael L. Szymanski

The Prairie Naturalist

Islands can provide secure nesting habitat for ducks and other waterbirds, especially in agriculturally dominated landscapes. I inventoried natural and man-made islands in the portion of North Dakota covered by the Prairie Pothole Joint Venture (PPJV). I mapped 1,305 islands in this area; up to 46% of which could provide enhanced nest success with management (e.g., predator removal or establishment of brushy cover). Management of islands for breeding ducks may be an important method for achieving desired reproductive rates in the PP JV as substantial areas of perennial grass cover are lost from federal conservation programs, primarily the Conservation Reserve …

Education In Plant Pathology Present Status And Future Challenges, James Macdonald, Caitilyn Allen, David Gadoury, William Jacobi, Segenet Kelemu, James Moyer, Tim Murray, Kevin Ong, Charles Pearson, John Sherwood, Ann Vidaver

Department of Plant Pathology: Faculty Publications

Plant pathology is largely a mission driven discipline that seeks to increase the fundamental understanding of host–pathogen interactions and the etiology of plant diseases for the purpose of preventing or mitigating crop loss. This is an important mission because all nations face serious challenges in their efforts to prevent disease on plants cultivated for food, fiber, ornamental use, timber, and fuel, as well as those growing in native ecosystems. In 1994, it was estimated that global losses due to plant diseases ranged from 9.7 to 14.2% of potential yield (12).

Herbicide, Salinity, And Flooding Tolerance Of Foxtail Barley (Hordeum Jubatum L.) And Desirable Pasture Grasses, Karl R. Israelsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Research trials performed in the greenhouse compared the tolerance and response of Hordeum jubatum and desirable pasture grass species to herbicides, salinity, and flooding. Desirable grass species used in this study included: 'Fawn' tall fescue (Festuca arundinaceae), 'Garrison' creeping foxtail (Alopecurus arundinaceus), 'Palaton' reed canarygrass (Phalaris arundinacea), 'Climax' timothy (Phleum pratense), 'Alkar' tall wheatgrass (Thinopyrum ponticum), 'Potomac' orchardgrass (Dactylis glomerata), and 'Mustang' altai wildrye (Leymus angustus). Tolerance to herbicides, salinity, and flooding varied significantly among grass species. Herbicide tolerance was tested using four herbicides at five …

Techniques For Triggering Germination Of Adenostoma Fasciculatum In Revegetation Projects At Rocky Canyon Quarry, Stephanie B. Scolari

Master's Theses

Rocky Canyon Quarry is a granite mine located in the Santa Lucia Mountains about 5 miles southeast of the city of Atascadero. The Surface Mining and Reclamation Act (SMARA), which passed in 1975, dictates that California mines must rehabilitate lands disturbed by mining operations. Dr. V. L. Holland and his students in the Biological Sciences Department at California Polytechnic State University, San Luis Obispo (Cal Poly) have been working on restoration and revegetation of the Rocky Canyon Quarry since the early 1990’s.

Adenostoma fasciculatum (chamise) dominates the chaparral communities (chamisal chaparral) found in Rocky Canyon. Chamise is the most common …

The Malawi Project: From Conventional To Holistic Decision Making, Grace Wetmore

Animal Science

How the Cal Poly Malawi Appropriate Technologies Team, and other development groups, can use Holistic Management to aid developing countries in an effort towards a sustainable future.

Water Uptake Threshold Of Rabbiteye (Vaccinium Ashei) Blueberries And Its Influence On Fruit Splitting, Donna A. Marshall-Shaw, James M. Spiers, Kenneth J. Curry

Faculty Publications

Split-resistant and split-susceptible rabbiteye blueberry fruit were evaluated at all stages of development to determine ''water uptake thresholds'' by soaking in distilled water. Weight increase after soaking was measured, and percent weight gain was calculated to take into consideration the weight increase of the fruit from development. The ratio of percent increase in volume to weight increase resulting from water uptake was calculated. Ratios of percent water uptake to weight increase between splitsusceptible 'Tifblue' and split-resistant 'Premier' blueberries were found to be similar. The split-susceptible 'Tifblue' had a 1.6 g/50 fruit increase with a 1.7% water uptake and a ratio …

Observations Of Badgers Preying On Black-Tailed Prairie Dogs, Daniel S. Licht

The Prairie Naturalist

American badgers (Taxidea taxus) often visit black-tailed prairie dog (Cynomys ludovicianus) colonies, most likely in search of prey (Lomolino and Smith 2004, Shaughnessy and Cifelli 2004). Badgers are well suited to hunting fossorial prey such as prairie dogs by excavating burrows and capturing individuals belowground (Lindzey 2003). However, the ecological literature is sparse regarding details of how badgers hunt and capture prairie dogs underground. Eads and Biggins (2008) documented three occurrences of a badger excavating prairie dogs. That badger (apparently the same individual) had a den within the prairie dog town where captures occurred. This note …

Mortality Of Cranes (Gruidae) Associated With Powerlines Over A Major Roost On The Platte River, Nebraska, Gregory D. Wright, Timothy J. Smith, Robert K. Murphy, Jeffery T. Runge, Robert R. Harms

The Prairie Naturalist

Two 69-kilovolt powerlines spanning the Platte River in south central Nebraska are suspected to cause substantial mortality to sandhill cranes (Grus canadensis) and pose a threat to endangered whooping cranes (G. americana) that roost overnight on the river during spring and fall migrations. Most studies of crane collisions with powerlines in the region have focused on counts of carcasses away from night roosts on the river and none have accounted for potential biases in detecting carcasses. We found 61 carcasses of sandhill cranes below over-river segments of the two powerlines during 4 March to 7 April …

Bed Site Selection Of Fawn Pronghorn In Custer State Park, South Dakota, Chad P. Lehman, Jamin D. Hartland, Barbara J. Keller, Joshua J. Millspaugh, Gary C. Brundige

The Prairie Naturalist

We evaluated pronghorn (Antilocapra americana) fawn bedding site characteristics on a prairie and ponderosa pine (Pinus ponderosa) landscape interface in Custer State Park, South Dakota. We radio-marked 16 adult female pronghorn and collected bed site information from their fawns during 2007~2008. We compared bed site selection with random sites (n = 74) during 2 periods; the early hiding phase when fawns were 1 ~28 days of age (n = 23 bed sites) and the later group phase when fawns were 29~60 days of age (n = 52 bed sites). During the hiding phase fawns selected dry …

Discovering Metabolic Networks Of Bovine Fertilization, Erin Lynn Young

Undergraduate Honors Capstone Projects

At the time of fertilization, a dramatic change occurs in the oocyte that transforms this cell from a metaphase arrested state into a metabolically active and dynamic state. The view of the flow of biological processes within organisms has recently shifted from that of a linear path to a more complex network. Biological processes are no longer thought of in the simple terms of DNA to RNA, RNA to proteins, and proteins to final activity. It is now known that many biological processes involve interconnected networks and feedback loops in which DNA, RNA, proteins, and metabolites perform specific roles. We …

Characterization Of Genes Encoding Poly(A) Polymerases In Plants: Evidence For Duplication And Functional Specialization, Lisa R. Meeks, Bala Subrahmanyam Addepalli, Arthur G. Hunt

Plant and Soil Sciences Faculty Publications

Background: Poly(A) polymerase is a key enzyme in the machinery that mediates mRNA 3′ end formation in eukaryotes. In plants, poly(A) polymerases are encoded by modest gene families. To better understand this multiplicity of genes, poly(A) polymerase-encoding genes from several other plants, as well as from Selaginella, Physcomitrella, and Chlamydomonas, were studied.

Methodology/Principal Findings: Using bioinformatics tools, poly(A) polymerase-encoding genes were identified in the genomes of eight species in the plant lineage. Whereas Chlamydomonas reinhardtii was found to possess a single poly(A) polymerase gene, other species possessed between two and six possible poly(A) polymerase genes. With the exception …

Non-Antibiotic Selection Systems For Soybean Somatic Embryos: The Lysine Analog Aminoethyl-Cysteine As A Selection Agent, Suryadevara S. Rao, Lewamy Mamadou, Matt Mcconnell, Raghuveer Polisetty, Prachuab Kwanyuen, David Hildebrand

Plant and Soil Sciences Faculty Publications

BACKGROUND: In soybean somatic embryo transformation, the standard selection agent currently used is hygromycin. It may be preferable to avoid use of antibiotic resistance genes in foods. The objective of these experiments was to develop a selection system for producing transgenic soybean somatic embryos without the use of antibiotics such as hygromycin.

RESULTS: When tested against different alternate selection agents our studies show that 0.16 microg/mL glufosinate, 40 mg/L isopropylamine-glyphosate, 0.5 mg/mL (S-(2 aminoethyl)-L-cysteine) (AEC) and the acetolactate synthase (ALS) inhibitors Exceed and Synchrony both at 150 microg/mL inhibited soybean somatic embryo growth. Even at the concentration of 2 mg/mL, …

Viral Vectors Useful In Soybean And Methods Of Use, Said A. Ghabrial, Chunquan Zhang, Honcgang Gu

Plant Pathology Faculty Patents

The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The invention also provides methods for expressing a heterologous polypeptide in a plant such as soybean. The invention additionally provides methods for virus-induced gene silencing, particularly in a soybean plant, which can be used to determine the function of a gene of interest.

Defective Interfering Rnas: Foes Of Viruses And Friends Of Virologists, Kunj Bihari Pathak, Peter D. Nagy

Plant Pathology Faculty Publications

Defective interfering (DI) RNAs are subviral RNAs produced during multiplication of RNA viruses by the error-prone viral replicase. DI-RNAs are parasitic RNAs that are derived from and associated with the parent virus, taking advantage of viral-coded protein factors for their multiplication. Recent advances in the field of DI RNA biology has led to a greater understanding about generation and evolution of DI-RNAs as well as the mechanism of symptom attenuation. Moreover, DI-RNAs are versatile tools in the hands of virologists and are used as less complex surrogate templates to understand the biology of their helper viruses. The ease of their …

Biological Control Of Purple Loosestrife (Lythrum Salicaria): Factors Affecting Galerucella Pusilla And Galerucella Calmariensis Establishment In Tidal Areas, Lynda Kathryn Moore

Dissertations and Theses

Galerucella pusilla and G. calmariensis have provided successful biological control of purple loosestrife (Lythrum salicaria L.) in non-tidal areas but only marginal control in areas of tidal influence. While a previous study identified mechanical scour by tidal waters as the main cause of establishment failure, purple loosestrife stem density explained more than 80% of the variability in presence and absence of Galerucella at my study sites in the Columbia River Estuary. A logistic regression model using purple loosestrife stem density, elevation, and their interaction as predictors accurately predicted 92.5% of Galerucella presence or absence observations of a test data …

Effect Of Different Schedules Of Baby Corn (Zea Mays L.) Harvests On Baby Corn Yield, Grain Yield, And Economic Profit Value, Zheng Wang

Masters Theses & Specialist Projects

Maize (Zea mays L.) ranks third as a food crop after wheat and rice and is characterized not only as a cereal crop but also as a vegetable. Maize used as a vegetable is known as “baby corn”. Baby corn consists of unfertilized young ears harvested 2 or 3 days after silk emergence. The present study was implemented in 2009 at Western Kentucky University Agriculture Research and Education Center (36.93 N, 86.47 E) in Bowling Green, Kentucky. The purpose of the study was to compare the effect of different schemes of harvest on baby corn (BC) yield, grain maize (GM) …

Ribosomal Protein L10 Is Encoded In The Mitochondrial Genome Of Many Land Plants And Green Algae, Jeffrey P. Mower, Linda Bonen

Center for Plant Science Innovation: Faculty Publications

Background: The mitochondrial genomes of plants generally encode 30-40 identified protein-coding genes and a large number of lineage-specific ORFs. The lack of wide conservation for most ORFs suggests they are unlikely to be functional. However, an ORF, termed orf-bryo1, was recently found to be conserved among bryophytes suggesting that it might indeed encode a functional mitochondrial protein.

Results: From a broad survey of land plants, we have found that the orf-bryo1 gene is also conserved in the mitochondria of vascular plants and charophycean green algae. This gene is actively transcribed and RNA edited in many flowering plants. Comparative sequence …

Nebline, November/December 2009

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Middle School Youth on a “Nutrition Mission”
Roasts – Easy to Make for the Holidays and Any Other Time, Too!
Family & Community Education (FCE) Clubs: Presidents’ Notes—Bonnie’s Bits
FCE News & Events
Household Hints: Get a Handle on Holiday Cleaning
Radon—A Health Hazard
Computer Cleaning—Hotspots for Germs
Flexible Cash Lease Provisions
Thistle Control Still Possible This Fall — but Hurry
Use Season-Long Records to Assess Pumping Plant Performance
Diversity is the Key to Attracting Wildlife
Provide Basic Needs to Attract Birds
Relocating Problem Wild Animals: Not as Humane as Most People Think
Evergreen Colors for Winter
Fall Vegetable Garden …

No-Till Tomato Production, Bradley L. Booker

Master's Theses

Tillage increases erosion rates and diminishes the quality of soils but has traditionally been a way to manage weeds and prepare a seedbed. No-till vegetable production can ameliorate the ill-effects of tillage in an economically effective way but has not been studied much in California. The objective of this thesis was to determine the viability of no-till vegetable cropping on the Central Coast of California. Tomatoes were grown in no-till and conventionally tilled treatments. Total yield, fruit weight, weed emergence, soil bulk density, soil aggregate stability, and soil organic carbon content were measured and compared. Yields and soil tests in …

Proximity To A Forest Leads To Higher Honey Yield: Another Reason To Conserve, Susan O. Sande, Robin M. Crewe, Suresh K. Raina, Susan W. Nicolson, Ian Gordon

All Peer-Reviewed Publications

Although tropical forest conservation is a top priority for human and environmental health, deforestation persists, mainly because of food and economic needs. No community will totally give up economic activities for the sake of ecological integrity, unless it is given alternative economic activities from which to draw its livelihood. Beekeeping in the forest buffer zone instead of traditional destructive honey-harvesting from forest trees is one such option at Arabuko Sokoke Forest (ASF) in Kenya. ASF is a dry coastal forest which is home to endangered and threatened fauna and is a hotspot considered a priority for conservation. In order to …

Genetic Insulator For Preventing Influence By Another Gene Promoter, Susheng Gan, Mingtang Xie

Plant and Soil Sciences Faculty Patents

A 16 bp polynucleotide sequence of Arabidopsis thaliana is a genetic insulator that can effectively isolate a transgene from positional effects of neighboring gene activities in transgenic plant cells.

Inhibition Of Rna Recruitment And Replication Of An Rna Virus By Acridine Derivatives With Known Anti-Prion Activities, Zsuzsanna Sasvari, Stéphane Bach, Marc Blondel, Peter D. Nagy

Plant Pathology Faculty Publications

BACKGROUND: Small molecule inhibitors of RNA virus replication are potent antiviral drugs and useful to dissect selected steps in the replication process. To identify antiviral compounds against Tomato bushy stunt virus (TBSV), a model positive stranded RNA virus, we tested acridine derivatives, such as chlorpromazine (CPZ) and quinacrine (QC), which are active against prion-based diseases.

METHODOLOGY/PRINCIPAL FINDINGS: Here, we report that CPZ and QC compounds inhibited TBSV RNA accumulation in plants and in protoplasts. In vitro assays revealed that the inhibitory effects of these compounds were manifested at different steps of TBSV replication. QC was shown to have an effect …

Insertion Sequence Content Reflects Genome Plasticity In Strains Of The Root Nodule Actinobacterium Frankia, Derek M. Bickhart, Johann P. Gogarten, Pascal Lapierre, Louis S. Tisa, Philippe Normand, David R. Benson

Molecular, Cellular & Biomedical Sciences

Background: Genome analysis of three Frankia sp. strains has revealed a high number of transposable elements in two of the strains. Twelve out of the 20 major families of bacterial Insertion Sequence (IS) elements are represented in the 148 annotated transposases of Frankia strain HFPCcI3 (CcI3) comprising 3% of its total coding sequences (CDS). EAN1pec (EAN) has 183 transposase ORFs from 13 IS families comprising 2.2% of its CDS. Strain ACN14a (ACN) differs significantly from the other strains with only 33 transposase ORFs (0.5% of the total CDS) from 9 IS families. Results: Insertion sequences in the Frankia genomes were …