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Regulation Of Septum Formation By The Bud3–Rho4 Gtpase Module In Aspergillus Nidulans, Haoyu Si, Daniela Justa-Schuch, Stephan Seiler, Steven D. Harris

Department of Plant Pathology: Faculty Publications

The ability of fungi to generate polarized cells with a variety of shapes likely reflects precise temporal and spatial control over the formation of polarity axes. The bud site selection system of Saccharomyces cerevisiae represents the best-understood example of such a morphogenetic regulatory system. However, the extent to which this system is conserved in the highly polarized filamentous fungi remains unknown. Here, we describe the functional characterization and localization of the Aspergillus nidulans homolog of the axial bud site marker Bud3. Our results show that AnBud3 is not required for polarized hyphal growth per se, but is involved in septum …

An In Vitro Method For The Analysis Of Infection-Related Morphogenesis In Fusarium Graminearum, William R. Rittenour, Steven D. Harris

Department of Plant Pathology: Faculty Publications

Fusarium graminearum is a significant pathogen of many cereal crops. With its genetic tractability, ease of culture, genome sequence availability and economic significance, F. graminearum has become the subject of intensive molecular research. Although molecular tools have been developed to enhance research into virulence determinants of F. graminearum, simple assays for infection-related development are lacking. As such, the objective of this study was to develop an in vitro protocol for the analysis of infection-related morphogenesis in F. graminearum. We demonstrate that two morphologically distinct hyphal structures are produced by F. graminearum during the invasion of detached wheat glumes: subcuticular hyphae …

Copy Number Variation Shapes Genome Diversity In Arabidopsis Over Immediate Family Generational Scales, Seth Debolt

Horticulture Faculty Publications

Arabidopsis thaliana is the model plant and is grown worldwide by plant biologists seeking to dissect the molecular underpinning of plant growth and development. Gene copy number variation (CNV) is a common form of genome natural diversity that is currently poorly studied in plants and may have broad implications for model organism research, evolutionary biology, and crop science. Herein, comparative genomic hybridization (CGH) was used to identify and interrogate regions of gene CNV across the A. thaliana genome. A common temperature condition used for growth of A. thaliana in our laboratory and many around the globe is 22 degrees C. …

Assessing Genetic Differentiation Among Populations Of The Invasive Plant Impatiens Glandulifera In Maine, Jordan R. Schoonover

Honors Theses

The annual herbaceous plant Impatiens glandulifera Royle is native to the Himalayas and is a significant invasive species in Europe. In the past century, it was introduced to the United States, where it has become established in 12 states. This study evaluated genetic differentiation among four Maine populations, to address a theory that posits hybridization of distinct lineages as a trigger for invasiveness. Regions of microsatellite repeats were evaluated at two polymorphic loci for 41 plants sampled from the four populations. A striking finding was that the observed heterozygosity was substantially higher than the heterozygosity expected from random combination of …

The Wheat Bzip Factor, Taabf1, Mediates Aba-Induced Gene Expression In Bombarded Barley Aleurone Layers, Benjamin R. Keyser

Honors Theses

The plant hormone Abscisic acid (ABA) plays a central role in maturation and germination in seeds, as well as mediating adaptive responses to abiotic environmental stresses. ABA induces the expression of many genes, including late-embryogenesis-abundant genes such as HVA1. To elucidate the ABA signaling pathway leading to HVA1 expression, we focus on the bZIP factor TaABF1. Analysis of the interplay between ABA and TaABF1 in the aleurone cells of imbibing cereal grains indicated that the two are not additive in their induction of the HVA1 promoter. A synthetic ABA analog, PBI-51, did not specifically inhibit the effect of exogenous ABA …

The Role Of Taabf1 In Abscisic Acid-Mediated Suppression Of 𝒶-Amylase Gene Expression In Cereal Grains, Lauren J. Harris

Honors Theses

The phytohormones gibberellin (GA) and abscisic acid (ABA) regulate important developments events in germinating seeds. Specifically, GA induces the expression of hyrolase genes, like the α-amylase gene Amy32b, which mobilizes starch reserves to be used by the embryo, and ABA suppresses this induction. Recent advancements identified ABA and GA receptors and key components in the signaling pathways, however, the mechanism of crosstalk between the hormones remains largely unknown. To further elucidate the mechanism of ABA suppression of GA-induced genes, we focused on the transcription factor TaABF1, a member of the ABA response element binding factor family. TaABF1 has been shown …

Science Based Authentication Of Dietary Supplements: Studies On The Chemical Constituents, Analytical Method And Biological Activities Of Scutellaria Spp And Pfaffia Paniculata, Jing Li

Electronic Theses and Dissertations

During the last decade, the use of herbal medicine has expanded globally and gained popularity. With the tremendous expansion in the use of herbal medicine worldwide, safety and efficacy as well as quality control of herbal medicines have become more and more issues of concern for both health authorities and the public. Although herbal medicine has been in use for hundreds to thousands years, very limited science-based data exist to explicit its chemical constituents, pharmacological activities and toxicity. Therefore, lack of scientific evidence for herbal medicine usage elicits the need for systematic studies of herbal medicine, including phytochemical, analytical, and …

Global Assessment Of Nitrogen Deposition Effects On Terrestrial Plant Diversity : A Synthesis, R. Bobbink, K. Hicks, J. Galloway, T. Spranger, R. Alkemade, M. Ashmore, M. Bustamante, S. Cinderby, E. Davidson, F. Dentener, B. Emmett, J. W. Erisman, M. Fenn, Frank S. Gilliam, A. Nordin, L. Pardo, W. Devries

Biological Sciences Faculty Research

Atmospheric nitrogen (N) deposition is a recognized threat to plant diversity in temperate and northern parts of Europe and North America. This paper assesses evidence from field experiments for N deposition effects and thresholds for terrestrial plant diversity protection across a latitudinal range of main categories of ecosystems, from arctic and boreal systems to tropical forests. Current thinking on the mechanisms of N deposition effects on plant diversity, the global distribution of G200 ecoregions, and current and future (2030) estimates of atmospheric N-deposition rates are then used to identify the risks to plant diversity in all major ecosystem types now …

Comparison Between In Vitro And In Vivo Antibacterial Activity Of Curcuma Zedoaria From Malaysia., Behrooz Banisalam

Student Works (2010-2019)

The rhizomes of the Zingiberaceae family are vegetables widely used in many Asian countries and their medicinal functions have been broadly discussed and accepted in many traditional recipes. In this study, the antimicrobial activity of extracts of Curcuma zedoaria from Malaysia was compared between two systems as in vitro and in vivo. The comparison was performed against four bacterial strains including two gram negative strains (E.coli and Pseudomonas aeruginosa) and two gram positive strains (Bacillus cereus and Staphylococcus aureus) using the agar well diffusion method. Extracts were made in three different solvents; petroleum ether, chloroform and methanol extracts exhibited antibacterial …

Development Of Protocols For Tissue Culture And Mutagenesis In Two Variegated Sansevieria Sp., Irmawati Akma Abdul Hapiz

Student Works (2010-2019)

Studies were conducted to develop the tissue culture protocol and mutagenesis through Gamma ray irradiation for two variegated Sansevieria species known as Sansevieria trifasciata var. Laurentii (goldband Sansevieria) and Sansevieria trifasciata var. Hahnii (birdnest Sansevieria). Seven combinations of BAP and NAA were chosen which are represented by letter A= 1.00 mg/I BAP, B = 1.00 mg/I BAP + 0.25 mg/I NAA, C = 2.00 mg/I BAP + 0.50 mg/I NAA, D = 2.00 mg/I BAP + 1.00 mg/I NAA, E = 3.00 mg/I BAP + 1.00 mg/I NAA, F = 4.00 mg/I BAP + 1.00 mg/I NAA and G = …

Fluid Fertilizer’S Role In Sustaining Soils Used For Bio-Fuels Production, John L. Kovar, Douglas L. Karlen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The short- and long-term effects on soil nutrient cycling, physical properties, and biological activity of striving for higher grain yields and removing crop residues for bio-fuels production must be understood to provide more quantitative crop and soil management guidelines. This study focuses primarily on potassium (K) and sulfur (S) response by corn (Zea mays L.) grown for bio-energy feedstock production. Our objectives for 2009 were to evaluate (i) the performance of several S fertilizers, including liquid ammonium thiosulfate (12-0-0-26S), for corn grown in Iowa, and (ii) the use of surface or subsurface bands of N-P-K-S fluid fertilizers to optimize …

Procedures For Publishing In Non-Usda Media (Outside Publishing)

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This P&P is an update of a previous version dated March 10, 1998. This P&P outlines revised responsibilities of the ARS author, Research Leader (RL), and other ARS officials. The most salient features of the new P&P are that the manuscript approval authority and associated responsibilities are delegated to the RL. ARS Form 115 (ARS-115) is revised accordingly (see Exhibit 1). Among the essential RL responsibilities are to determine the need and method for manuscript peer review, approve the technical and interpretive content of the manuscript, notify upper management about manuscripts containing prominent issues, obtain proper clearance/approval from upper management …

U.S. Meat Animal Research Center Clay Center, Ne

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mission

Research Units

U.S. Meat Animal Research Center Animal Health Research Unit

U.S. Meat Animal Research Center Environmental Management Research Unit

U.S. Meat Animal Research Center Genetics & Breeding Research Unit

U.S. Meat Animal Research Center Meat Safety and Quality Research Unit

U.S. Meat Animal Research Center Nutrition Research Unit

U.S. Meat Animal Research Center Reproduction Research Unit

Classical Biological Control For The Protection Of Natural Ecosystems, R.G. Van Driesche, R I. Carruthers, T. Center, M.S. Hoddle, J. Hough-Goldstein, L. Morin, L. Smith, D.L. Wagner, B. Blossey, V. Brancatini, R. Casagrande, C.E. Causton, J.A. Coetzee, J. Cuda, J. Ding, S.V. Fowler, J. H. Frank, R. Fuester, J. A. Goolsby, M. Grodowitz, T.A. Heard, M.P. Hill, J.H. Hoffmann, J. Huber, M. Julien, M.T.K. Kairo, M. Kenis, P. Mason, J. Medal, R. Messing, R. Miller, A. Moore, P. Neuenschwander, R. Newman, H. Norambuena, W.A. Palmer, R. Pemberton, A. Perez-Panduro, P.D. Pratt, M. Rayamajhi, S. Salom, D. Sands, S. Schooler, M. Schwarzländer, A. Sheppard, R. Shaw, P.W. Tipping, R.D. Van Klinken

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Of the 70 cases of classical biological control for the protection of nature found in our review, there were fewer projects against insect targets (21) than against invasive plants (49), in part, because many insect biological control projects were carried out against agricultural pests, while nearly all projects against plants targeted invasive plants in natural ecosystems. Of 21 insect projects, 81% (17) provided benefits to protection of biodiversity, while 48% (10) protected products harvested from natural systems, and 5% (1) preserved ecosystem services, with many projects contributing to more than one goal. In contrast, of the 49 projects against invasive …

Effects Of A New Waste-Processing By-Product On Soil And Vegetation At Fort Campbell, Tennessee, R. R. Busby, D. L. Gebhart, H. A. Torbert, J. O. Dawson, G. A. Bollero, K. N. Potter, D. R. Curtin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A garbage-processing technology has been developed that sterilizes and separates inorganic and organic components of municipal solid waste. A study was initiated to evaluate the uncomposted organic by-product of this process as a soil amendment for establishing native prairie grasses on disturbed Army training lands. The waste was incorporated into a silt loam soil at Fort Campbell Military Reservation in the central United States. The waste material was applied at rates of 0, 4.5, 9, 18, and 36 Mg ha^-1 and seeded with native prairie grasses to assess its effects on vegetation for two growing seasons, with an additional …

Combining A Dispersal Model With Network Theory To Assess Habitat Connectivity, Todd R. Lookingbill, Robert H. Gardner, Joseph R. Ferrari, Cherry E. Keller

Geography and the Environment Faculty Publications

Assessing the potential for threatened species to persist and spread within fragmented landscapes requires the identification of core areas that can sustain resident populations and dispersal corridors that can link these core areas with isolated patches of remnant habitat. We developed a set of GIS tools, simulation methods, and network analysis procedures to assess potential landscape connectivity for the Delmarva fox squirrel (DFS; Sciurus niger cinereus), an endangered species inhabiting forested areas on the Delmarva Peninsula, USA. Information on the DFS’s life history and dispersal characteristics, together with data on the composition and configuration of land cover on the peninsula, …

Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max) has undergone at least two rounds of polyploidization, resulting in a paleopolyploid genome that is a mosaic of homoeologous regions. To determine the structural and functional impact of these duplications, we sequenced two ~1-Mb homoeologous regions of soybean, Gm8 and Gm15, derived from the most recent ~13 million year duplication event and the orthologous region from common bean (Phaseolus vulgaris), Pv5. We observed inversions leading to major structural variation and a bias between the two chromosome segments as Gm15 experienced more gene movement (gene retention rate of 81% in Gm15 versus 91% in …

Elymus Sosnowskyi (Hackel) Melderis (Poaceae), A Rare Endemic Species In Turkey, Evren Cabi̇, Musa Doğan, Özlem Mavi̇, Ersi̇n Karabacak, Bi̇rol Başer

Turkish Journal of Botany

Elymus sosnowskyi, a rare endemic species known from East Anatolia, Turkey, was first collected by D. Sosnowsky in 1912. After that it has been collected only one other time, by F. Sorger in 1981. During the "Taxonomic revision of tribe Triticeae in Turkey" project, which was supported by TÜBİTAK, E. sosnowskyi was recollected from 3 populations in Oltu and Narman (A8 Erzurum, Turkey) in 2007 and 2008. The present study aimed to provide detailed diagnostic characters of this rare endemic species, including its morphological, anatomical, and palynological features. Moreover, the amended and expanded description, distribution, phenology, and ecology of this …

Anomodon Longifolius (Anomodontaceae, Bryopsida) New To The Bryophyte Flora Of Turkey, Muhammet Ören, Güray Uyar, Tamer Keçeli̇

Turkish Journal of Botany

Anomodon longifolius (Schleich. ex Brid.) Hartm. is reported for the first time from Turkey, bringing the total number of Anomodon species known from Turkey to 6. This record extends its distribution range to the Western Black Sea region of Turkey. The nearest localities to Turkey for this species are in Bulgaria, Greece, the Caucasus, and Iran. This submeridional species was collected from 3 different localities (in Bolu and Bartın provinces) in Turkey. A site description, illustrations, and diagnostic characters of the Turkish specimens are given, together with notes on its distribution and ecology.

Ovule Ontogenesis And Megagametophyte Development In Onobrychis Schahuensis Bornm. (Fabaceae), Abdolkarim Chehregani, Nayereh Tanomi

Turkish Journal of Botany

The ovule ontogenesis and the megasporogenesis events in Onobrychis schahuensis Bornm. were studied with light microscopy. The primordium is tetra-zonate and gives rise to an anatropous ovule. The archesporium may consist of one or more archesporial cells, but only one of them undergoes meiosis, forming a linear shaped tetrad. Normally, only a single megaspore is functional, which is located in the chalazal position, while the others degenerate very soon. The young ovule is hemianatropous but the mature one is anatropous, crassinucellar, and bitegmic; integuments form a zig-zag micropyle. A 7-celled embryo-sac is formed corresponding to the Polygonum type. The present …

Macrofungi Of Hatila Valley National Park (Artvin, Turkey), Kenan Demi̇rel, Ömer Erdem, Yusuf Uzun, Abdullah Kaya

Turkish Journal of Botany

The taxonomic study of 382 samples collected from Hatila Valley National Park (Artvin, Turkey) revealed 126 taxa of macromycetes, belonging to 34 families and 60 genera. Two of them, Arrhenia acerosa (Fr.) Kühn. (Tricholomataceae) and Entoloma politum (Pers.: Fr.) Donk. (Entolomataceae), are new records for Turkey.

Phylogenetic Relationships Of Some Turkish Crocus (Iridaceae) Taxa Based On Morphological And Anatomical Characters, Fati̇h Coşkun, Selami̇ Selvi̇, Fati̇h Satil

Turkish Journal of Botany

In this study, relationships among the 15 taxa of the genus Crocus L. distributed in Turkey were analysed using 29 morphological and 4 anatomical characters. Analysis of the data set utilising maximum parsimony criterion with Branch-and-Bound search algorithm yielded 32 most parsimonious trees. Bootstrap analysis with the majority rule consensus algorithm generated a consensus tree supporting some branches. Our data mostly did not agree with the previous sectional and serial treatments. The most interesting result was the condition of Crocus pallasii, which was previously included in the section Crocus. C. pallasii showed a sister group relationship with C. cancellatus from …

A New Record For Turkey: Michauxia Nuda A.Dc. (Campanulaceae), Serdar Aslan, Meci̇t Vural, Bi̇lal Şahi̇n, Esra Ergi̇n, Ömer Faruk Kaya

Turkish Journal of Botany

Michauxia nuda A.DC. (Campanulaceae) is recorded for the first time (C8 Siirt province) from Turkey. The diagnostic characters of M. nuda are described and its habitat and conservation status are indicated. Besides, it is illustrated, and a distribution map is presented. Notes on collecting number of type specimen of this species are provided.

A New Record For The Flora Of Turkey: Scorzonera Ketzkhovelii Grossh. (Asteraceae), Ergi̇n Hamzaoğlu, Ahmet Aksoy, Esra Marti̇n, Münevver Pinar, Hati̇ce Çölgeçen

Turkish Journal of Botany

Scorzonera ketzkhovelii Grossh. (Asteraceae) was recently collected from Yusufeli, Artvin (north-east Anatolia) and it has been reported as a new species record for the flora of Turkey. It is described and illustrated. In addition, its karyological features and pollen morphology are given.

New Additions To The Turkish Macromycota From Bingöl Province (Turkey), Yusuf Uzun, Abdullah Kaya, Mustafa Emre Akçay, Kenan Demi̇rel

Turkish Journal of Botany

Hymenoscyphus scutula (Pers.) W. Phillips, Crepidotus vulgaris Hesler & A.H. Sm., Psilocybe merdicola Huijsman, and Tricholoma inamoenum (Fr.) Gillet are recorded for the first time from Turkey. Short descriptions and photographs of the taxa are given.

Lophanthus (Lamiaceae) In Turkey: A New Generic Record And A New Species, Tuncay Di̇rmenci̇, Bayram Yildiz, Ian C. Hedge, Mehmet Firat

Turkish Journal of Botany

The genus Lophanthus Adans. is recorded for the first time from Turkey. Specimens collected from Van province, east Anatolia, are described here as the new species Lophanthus turcicus Dirmenci, Yıldız & Hedge. Diagnostic characteristics that distinguish it from allied species are presented; a description, distribution map, and taxonomic comments are given.

A New Subspecies Of Stachys Cretica (Section Eriostomum, Lamiaceae) From Turkey, Ekrem Akçi̇çek

Turkish Journal of Botany

Stachys cretica L. subsp. kutahyensis Akçiçek subsp. nova is described as a new subspecies from West Anatolia, Turkey. It is related to Stachys cretica L. subsp. vacillans Rech. f. and Stachys cretica L. subsp. anatolica Rech. f., from which it differs in stem, leaf, and flower characters. Diagnostic characters, description, detailed illustrations, and taxonomic comments of the new subspecies are given. The geographical distribution of the subspecies is mapped. IUCN threat category and observations on the population are noted.

Reseda Globulosa (Resedaceae): A New Record For The Flora Of Turkey, Zeki̇ Aytaç, Hayri̇ Duman

Turkish Journal of Botany

Reseda globulosa Fisch. & C.A.Mey. (Resedaceae) is reported as a new record for Turkish flora. It was identified using the collected specimens and Flora Palaestina. The description, illustration, and distribution of the species are given.

Internal Transcribed Spacer (Its) Polymorphism In The Wild Primula (Primulaceae) Taxa Of Turkey, Mutlu Gültepe, Uğur Uzuner, Kami̇l Coşkunçelebi̇, Ali̇ Osman Beldüz, Sali̇h Terzi̇oğlu

Turkish Journal of Botany

Twenty-three populations belonging to 10 wild Primula L. (Primulaceae) taxa of Turkey, some of which are morphologically quite similar, were investigated based on nrDNA ITS regions. The plant materials were collected from different geographical areas of Anatolia in vegetation periods of 2005 and 2006. Total genomic DNAs were isolated from the healthy leaves of each population. The entire ITS regions of the populations were amplified by universal primers with the aid of a polymerase chain reaction (PCR), and then the PCR products were sequenced. Neighbour-Joining (NJ) and Maximum Parsimony (MP) trees were constructed in order to identify the relationships among …

Morphological, Karyological And Phylogenetic Evaluation Of Cyclotrichium: A Piece In The Tribe Mentheae Puzzle, Tuncay Di̇rmenci̇, Ekrem Dündar, Görkem Deni̇z, Turan Arabaci, Esra Marti̇n, Zi̇ba Jamzad

Turkish Journal of Botany

The genus Cyclotrichium, a member of the tribe Mentheae subtribe Menthinae (Lamiaceae, Nepetoideae), was analysed with respect to morphological revision, phylogenetic analysis, and cytogenetic properties. All species of the genus were investigated for morphological characters and ITS (internal transcribed spacers) of nrDNA sequence comparison (except C. hausknechtii for ITS). Six members of the genus were also analysed for chromosome numbers. The combined results strongly suggested that Cyclotrichium is a separate genus in Nepetoideae with distinct morphological, phylogenetic, and cytogenetic characteristics. For intrageneric phylogeny of Cyclotrichium, 3 groups were recognised: 1. C. niveum; 2. C. origanifolium; and 3. the remaining 6 …