Validation Of Pcr-Based Assays And Laboratory Accreditation For Environmental Detection Of Aquatic Invasive Species, Invasive Species Advisory Committee

National Invasive Species Council

This white paper provides:

a) Background information on the use, accuracy and reliability of PCR-based assays such as environmentally sampled DNA (eDNA) for early detection of aquatic invasive species (AIS) and;

b) Recommendations for establishing a system for validating assays and accrediting laboratories that report on the presence or absence of AIS.

This white paper was developed by the members of ISAC and discusses the need for developing validation requirements for Polymerase Chain Reaction (PCR) and other DNA-based molecular assays that are increasingly being used to detect AIS. It does not provide a simplified checklist for evaluation of their ability …

Invasive Species And E-Commerce, Invasive Species Advisory Committee

National Invasive Species Council

ISSUE

Internet commerce (hereafter e-commerce) is a growing and vital part of the United States economy. Total e-commerce sales in the United States for 2011 totaled $194 billion, an increase of 16% over 2010. From 2002 to 2011, the proportion of reported e-commerce sales in the U. S. grew from about 1.4% to 5.5% of total retail sales (United States Census Bureau News 2012). Globally, e-commerce is expected to increase at a rate of 13.5% annually, amounting to $1.4 trillion in yearly sales by 2015 (Enright 2011). A portion of this activity includes the sale and trade of living organisms. …

Herbaceous 2012 (May 19), Amy L. Myers, Julia I. Chapman, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

Herbaceous vegetation data collected in 2012 at Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.

Traversing Swanton Road, 8th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …

In Vitro Regeneration Of Rudbeckia Hirta ‘Plainview Farm’ From Leaf Tissue, Youping Sun, Lijuan Han, Donglin Zhang

Plants, Soils, and Climate Faculty Publications

Rudbeckia hirta ‘Plainview Farm’, a new multiple-layered ray flowered cultivar, shows potential for potted plant production. After years of seed germination, this specific flower morphological trait was still unstable from generation to generation. To maintain its unique features, leaf sections (0.25 cm2 ) were cultured on Murashige and Skoog (MS) medium supplemented with either BA (0.5, 1.0, or 2.0 mg·L1 ), KIN (2.5, 5, or 10 mg·L-1 ), or ZT (0.5, 1.0, or 2.0 mg·L-1 )toinduce callus and microshoots. After cultivation for 33 days, all cytokinin treatments significantly induced callus and the callus size were 1.5- to-2.4-fold bigger than those …

Escape From Preferential Retention Following Repeated Whole Genome Duplications In Plants, James C. Schnable, Xiaowu Wang, J. Chris Pires, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

The well supported gene dosage hypothesis predicts that genes encoding proteins engaged in dose–sensitive interactions cannot be reduced back to single copies once all interacting partners are simultaneously duplicated in a whole genome duplication. The genomes of extant flowering plants are the result of many sequential rounds of whole genome duplication, yet the fraction of genomes devoted to encoding complex molecular machines does not increase as fast as expected through multiple rounds of whole genome duplications. Using parallel interspecies genomic comparisons in the grasses and crucifers, we demonstrate that genes retained as duplicates following a whole genome duplication have only …

Monitoring Ds Transposition In The Soybean Genome, Manmeet Singh

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Soybean (Glycine max (L.) Merrill) is a major oilseed commodity which partitions carbon and nitrogen flux during embryogenesis towards two primary storage reserves, protein and oil, at approximately 40% and 20%, respectively in the seed. This attribute makes soybean a valuable feedstock in many food, feed and industrial applications. Over the past decade, a wealth of genomic resources has been established for soybean that will aid in elucidating the underlying biology governing the growth and development of the crop. This in turn will foster innovative breeding and genetics approaches leading to improvements in agronomics and end-use quality. Loss- and …

Herbaceous 2012 (May 5), Amy L. Myers, Julia I. Chapman, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

Herbaceous vegetation data collected in 2012 at Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.

Cholesterol Lowering Effects Of Milk With Added Phytosterols, Laura Kells Cusack

Master's Theses

Foods incorporating plant sterols (PS) reduce Low-density lipoprotein (LDL) on average approximately 10%. PS with a higher lipid solubility may promote greater reductions. We examined the cholesterol lowering effect of a novel triglyceride recrystallized phystosterol (TRP). Twenty subjects (mean ± SD; age, 56 ± 10 years; BMI, 27 ± 5) with elevated LDL (>100 mg/dL) participated in three 4-week phases; Phase I, 2% milk; Phase II, milk with 2.0 grams (g) free PS; Phase III, milk with 2.0 g TRP. Before and after each phase two fasting blood draws were obtained for determination of serum cholesterol. Between the 2% …

An Investigation Of Fraxinus Americana Branch Sway Using A 3 Dimensional Motion Capture System, Anna T. Campiformio

Master's Theses

Abstract

Wind is a dominant abiotic factor that a tree experiences throughout its lifetime and can cause severe tree damage, resulting in risk of injury to humans, and economic and ecological losses. It is thought that trees develop structural properties and architectures that help withstand loading conditions by dissipating wind energy through damping mechanisms. The role of branch motion in reducing potential dangerous wind loads has been the focus of relatively few studies. Even fewer studies have examined tree sway response to natural wind loaded conditions. In this investigation, branch frequencies were calculated for three Fraxinus americana using a three-dimensional …

Ars Assists In Fight Against Kudzu Bug, Jan Suszkiw, Walker Jones

Agricultural Research Magazine

Sure, this distant relative of the brown marmorated stink bug will feed voraciously on the stems of kudzu, the “Vine That Ate the South.” But Megacopta cribraria also has a taste for soybean and other legumes. In Georgia, where this native of Asia was first discovered in the United States in October 2009, there’s worry that the pest will set its sights on peanut, endangering a $2 billion crop that supplies nearly 50 percent of America’s peanuts (Georgia Peanut Commission, 2009).

Like the brown marmorated stink bug, Megacopta—also known as the “bean plataspid”—seeks shelter inside homes, buildings, and vehicles …

Agricultural Research Magazine May-June 2012

Agricultural Research Magazine

Agricultural Research

150 Years of Making History

USDA's 150th Anniversary- May 15, 2012

Mutation And Complementation Of A Cellulose Synthase (Cesa) Gene, Ahmed Y. El-Araby

Senior Honors Projects

Cellulose is a carbohydrate polymer that is composed of repeating glucose subunits. Being the most abundant organic compound in the biosphere and comprising a large percentage of all plant biomass, cellulose is extremely plentiful and has a significant role in nature. Cellulose is present in plant cell walls, in commercial products such as those made from wood or cotton, and is of interest to the biofuel industry as a potential alternative fuel source. Although indigestible by humans, cellulose is nutritionally valuable, serving as a dietary fiber. Because of its ubiquity and importance in many areas, studying cellulose will prove to …

An Exploration Into Project Management: A Grassroots Experience In A Developing Nation, Brittany L. O'Brien

Senior Honors Projects

As a senior at the University of Rhode Island, it has been my experience that the knowledge that has been most successfully retained within my mind has been that which I gained through active learning. As an international development minor I have taken many courses on how developmental aid is provided around the world, and throughout these courses I have learned that the drive for creating those endeavors came from an understanding of need. In order to best complete my education on international development I created a sustainable developmental aid project which provided education for specific needs in Malawi, Africa. …

The Herring River Estuary: Understanding Salt Marsh Restoration And How To Predict The Response Of Phragmites Australis To Increased Tidal Flow, Theresa Murphy

Senior Honors Projects

My presentation invites you to learn about what is involved in planning a salt marsh restoration project. The Herring River Estuary is a disturbed salt marsh ecosystem at Cape Cod National Seashore which has been diked since 1909. The National Park Service (NPS), in cooperation with many partners, is engaged in efforts to restore the now impacted 1,100 acre estuary by re-introducing tidal flow. There is significant complexity involved in trying to restore such an intricate system- from the multitude of environmental factors to important social and economic consequences. There are also several benefits to restored tidal flow. My thesis …

Genotypic And Phenotypic Diversity Of Pyricularia Oryzae In The Contemporary Rice Blast Pathogen Population In Arkansas, Lu Zhai

Graduate Theses and Dissertations

Rice blast, caused by Pyricularia oryzae (teleomorph: Magnaporthe grisea), is one of the most economically important diseases of rice worldwide, including Arkansas. Rice blast has been severe the past few years on conventional cultivars and, more recently, has been observed on hybrid rice cultivars. The first objective of the current research was to examine the genotypic and phenotypic variation in the P. oryzae population in Arkansas during the 2009, 2010, and 2011 growing seasons and compare isolates from conventional cultivars with those from and hybrids. A total of 904 isolates were recovered from symptomatic rice cultivars in Arkansas and …

Cheating Cheatgrass: New Research To Combat A Wily Invasive Weed, Gail Wells

Joint Fire Science Program Digests

Cheatgrass and its cousin, red brome, are exotic annual grasses that have invaded and altered ecosystem dynamics in more than 41 million acres of desert shrublands between the Rockies and the Cascade-Sierra chain. A fungus naturally associated with these Bromus species has been found lethal to the plants’ soil-banked dormant seeds. Supported by the Joint Fire Science Program (JFSP), researchers Susan Meyer, Phil Allen, and Julie Beckstead cultured this fungus, Pyrenophora semeniperda, in the laboratory and developed an experimental field application that, in some trials, killed all the dormant soil-banked Bromus seeds, leaving none to germinate the following year. The …

Establishment Method And Cultural Practice Effects On Sports Turf, Josh Anderson

Graduate Theses and Dissertations

The use of soil-based sod on sand-based rootzones for the establishment of sports fields and golf courses is a common occurrence. Furthermore, the use of soil-based sod on sand-based rootzones can lead to potential problems associated with decreased water infiltration, gas exchange, rooting, and turfgrass quality caused by soil layering. Core-aerification and sand topdressing may help alleviate the problems associated with soil layering from the use of soil-based sod on sand-based rootzones. Therefore, the objectives of this research were to evaluate the effects of sod establishment methods and post- establishment cultural practices on the infiltration rate, moisture content, divot resistance, …

The Evolution Of Rural Farming In The Scottish Highlands And The Arkansas Delta: Investments And Inequalities, Madalyn Watkins

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

The development and evolution of an agricultural system is influenced by many factors including binding constraints (limiting factors), choice of investments, and historic presence of land and income inequality. This study analyzed the development of two farming systems: mechanized, “economies of scale” farming in the Arkansas Delta and crofting in the Scottish Highlands. The study hypothesized that the current farm size in each region can be partially attributed to the binding constraints of either land or labor. In Scotland, it was found that the continuous binding constraint was the availability of arable land. In Arkansas, the binding constraint began as …

Moss Mural, Chelsea Fredrikson

Senior Honors Projects

Moss Mural: Bridging the Arts and Sciences

Chelsea Fredrikson

Major: Fine Arts

Advisor: Robert Dilworth

Advisor Department: Fine Arts

Date 5-2012

Abstract

When we are young, in elementary school, we are encouraged to learn subjects together. Fine arts, science, math, and literature all work as one, and overlap to teach us valuable lessons and tools we can use as we grow. But as we become older school systems segregate subjects, forcing students to chose a direction. Do I have a creative mind, or a logical mind?

As an art student I’ve tried over the course of my college …

Exploring The Function Of Gt2 In Physcomitrella Patens, Tess Scavuzzo-Duggan

Senior Honors Projects

Plant cell walls are composed of a variety of carbohydrates, among them cellulose, pectin and hemicellulose. Cellulose is deposited in the cell wall as microfibrils via cellulose synthesis complexes (CSCs). These complexes contain the cellulose synthase proteins (CESAs) and come in two different morphological forms: rosettes and linear complexes. Rosette shaped cellulose synthesis complexes occur in land plants, whilst linear complexes are commonly found in red algae. However, some land plants, notably bryophytes (mosses) and seedless vascular plants, contain genes that encode both CESAs of the type that form rosette CSCs and also genes similar to those found in red …

Identification And Confirmation Of Ssr Markers And Qtl For Seed Calcium Content And Hardness Of Soybean, Moldir Orazaly

Graduate Theses and Dissertations

The health benefits and the nutritional values of soyfood products have expanded the demand for food-grade soybean varieties. For whole soyfood products such as natto, small seeded varieties with proper texture are essential. Seed calcium content and hardness are main factors in determining the seed texture. Utilizing molecular markers associated with quantitative trait loci (QTL) for marker assisted selection (MAS) has been an efficient technique for breeders. To find molecular markers associated with a trait is an initial step. The confirmation of QTL in different environments and in different genetic backgrounds is important for MAS. The objectives of this research …

Nebline, May 2012

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Lincoln’s Biosolids Land Application Program is 20 Years Old

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Principles Of Carbon Catabolite Repression In The Rice Blast Fungus: Tps1, Nmr1-3, And A Mate–Family Pump Regulate Glucose Metabolism During Infection, Jessie Fernandez, Janet D. Wright, David E. Hartline, Cristian Fernando Quispe, Nandakumar Madayiputhiya, Richard A. Wilson

Fungal Molecular Plant-Microbe Interactions

Understanding the genetic pathways that regulate how pathogenic fungi respond to their environment is paramount to developing effective mitigation strategies against disease. Carbon catabolite repression (CCR) is a global regulatory mechanism found in a wide range of microbial organisms that ensures the preferential utilization of glucose over less favourable carbon sources, but little is known about the components of CCR in filamentous fungi. Here we report three new mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae: the sugar sensor Tps1, the Nmr1-3 inhibitor proteins, and the multidrug and toxin extrusion (MATE)-family pump, Mdt1. Using simple plate …

Modifying The Fatty Acid Profile Of Soybean Oil For Nutritional And Industrial Applications, Hyunwoo Park

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The objectives of this study are: 1. To develop of soybean oil low in palmitic acid and elevated in stearic and oleic acids for margarine source. 2. To produce the high omega-3 fatty acid and astaxanthin in soybean seed as a feedstock for aquaculture. Over expression of mangosteen stearoyl-ACP thioesterase gene increased stearic acid content up to 10% to 15%. The identified elevated stearic acid events were crossed with our high oleic acid/low palmitic acid event. Average stearic and oleic acid content ranged 14 to 19% and 68 to 73% respectively. Stearic acid content of elevated stearic acid soybean event …

The Role Of Ascorbic Acid In The Plant-Herbivore Interaction, Kelly Ann Carruthers

Graduate Theses and Dissertations

Ascorbic acid (AsA), vitamin C, is an important molecule that is vital for both human and plant health, because it aids in the detoxification of reactive oxygen species (ROS) caused by various abiotic and biotic stresses including UV, salinity, ozone, and attack from pathogens In order to understand the role of AsA in the plant-herbivore interaction, we looked at the effects of the wound-responsive hormones, jasmonate, wounding, and herbivory on AsA content. Our study is the first to directly compare the effects of wounding and jasmonates on AsA levels in plants. We looked at the effects of wounding and methyl …

Effect Of Water-Deficit Stress On Cotton During Reproductive Development, Dimitra Loka

Graduate Theses and Dissertations

Water deficit is a major abiotic factor limiting plant growth and crop productivity around the world. Cotton (Gossypium hirsutum L.) is considered to be relatively tolerant to drought and the effects of water stress on leaf physiology and metabolism have been extensively documented. However, information is lacking on the effect of water-deficit stress on the cotton flower. It was hypothesized that water-deficit stress would impair gas exchange functions which consequently would result in perturbation of carbohydrates of cotton reproductive units. To investigate this hypothesis growth room studies and field studies were conducted with the objectives being to document the physiological …

Integrated Management Of Common Reed Along The Platte River In Nebraska: Control, Timing, Water Use, And Economic Analysis, Ryan E. Rapp

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

There are two biotypes of common reed, which includes the native common reed (Phragmites australis subsp. americanus) and non-native (invasive) common reed (Phragmites australis subsp. australis).The non-native biotype of common reed has invaded wetland habitats in many states of the US, including Nebraska.

Three studies, disking followed by herbicide, mowing followed by herbicide, and herbicide followed by mechanical treatment were initiated in 2008 in Nebraska. The objective was to evaluate common reed control along the Platte River using an integrated management. Herbicide followed by mechanical treatment had excellent control (≥92%) with all treatments except glyphosate applied …

Comparison Of Methods And Corn Root Types For Efficient Extraction Of Endoparasitic Nematodes, Jae L. Behn

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Endoparasitic nematodes annually reduced the yield of corn in the United States. Pratylenchus spp. and Hoplolaimus spp. are endoparasitic nematode genera that parasitize corn in the Midwest. Previous research has shown nematode population densities to be highly variable and extraction methods may not provide consistent results. In order to determine more consistent and time efficient results, comparisons were made between four standard nematode extraction techniques for preferences of nematode genera for extraction method, corn root type, and extraction time. The extraction methods evaluated were aeration-incubation, a modified Baermann funnel, Seinhorst’s mister, and shaker incubation. Research plots were established at two …

Stocking Density Affects Trampling And Use Of Vegetation On Nebraska Sandhills Meadow, Jordan R. Johnson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Ultrahigh stocking density (113,000 kg·ha^-1 to as high as 1 million kg·ha^-1 of beef) or mob grazing has been suggested to build soil, increase forage production and plant diversity, and improve grazing distribution compared to less intensive grazing systems. Experimental evidence does not completely support such conclusions. The overall focus of this research is based on the approach of building soil by optimizing above ground plant growth coming in contact with soil surface by trampling 60% of above ground plant growth. The objective of the study was to compare the effect of mob grazing and simple rotational grazing …