Digital Commons Network^™

La Serreta Endokarst (Se Spain): A Sustainable Value?, Antonia D. Asencio, Teodoro Espinosa

International Journal of Speleology

La Serreta endokarst (SE Spain), which UNESCO declared a World Heritage Site in 1998, was considered a sanctuary with cave art and one of the most important archaeological sites in the Mediterranean region for both the remains it hosts and the spectacular karstic landscape at the site.

To coincide with the 40th anniversary of its discovery, the La Serreta cave-chasm was adapted for public use with the intention of showing visitors the remains, which date back to prehistoric times. The solution included attempts to minimize contact with the valuables in the cave in order to alter the existing remains as …

Verticordia Micropropagation Through Direct Ex Vitro Rooting, Belinda Delaney

Theses: Doctorates and Masters

The objective of this study was to improve the existing shoot multiplication protocol for Verticordia grandis (McComb, Arthur & Newll, 1986; Newell, Growns & McComb, 2005) and to investigate and establish reliable root induction and acclimatisation protocols to enhance survival of micropropagated plantlets. It was envisaged that these protocols would be successful in micropropagation, growth and survival of different V. grandis clones and possibly applicable to other Verticordia species.

The elongation of in vitro Verticordia shoots on multiplication media was improved by reducing the concentration of BAP from 1μM to 0.25 μM, which resulted in a more uniform shoot length …

Influence Of Soil Nutrients On Reproduction And Pathogenicity Of Rotylenchulus Reniformis On Cotton, Herath Mudiyanselage Manjula Thaminda Kularathna

LSU Master's Theses

Among the variety of pathogens of cotton (Gossypium hirsutum), nematodes play a major role in reducing yield. Across the U.S. cotton belt, millions of dollars are lost annually due to nematode infestation. In the Mid-South and Southeast United States, root-knot nematode (Meloidogyne incognita) and reniform nematode (Rotylenchulus reniformis) are responsible for the highest percentage of damage. Crop rotation and nematicides are currently the most commonly used management strategies for nematode management. Soil fertility, which has a direct effect on plant growth, is also known to influence disease severity. Therefore, soil fertility would be an additional factor to consider for management …

A Phylogenetic Analysis Of Species Diversity, Specificity, And Distribution Of Mycodiplosis On Rust Fungi, Donald Jay Nelsen

LSU Master's Theses

There are more than 7800 species of Pucciniales (rust fungi) described. Aeciospores and urediniospores of rust fungi are a food source for the larval stage of members of the fly genus Mycodiplosis, hence these could be of interest as potential biological control agents. Currently, Mycodiplosis contains 49 described species based on adult male morphology. A survey of 1,350 rust-infected plants from 44 countries was recently conducted to assess the occurrence of Mycodiplosis fly larvae across a broad spectrum of Pucciniales. Larvae were found on 261 collections from 25 countries. Statistical analyses explored the distribution of larvae in relation to host …

Sweetpotato Storage Root Rots: Flooding-Associated Bacterial Soft Rot Caused By Clostridium Spp. And Infection By Fungal End Rot Pathogens Prior To Harvest, Washington Luis Da Silva

LSU Master's Theses

Sweetpotato production in the southern United States is being threatened by a soft rot that develops in storage roots when fields are flooded and by an important post-harvest disease caused mainly by the fungi Fusarium solani and Macrophomina phaseolina. To identify the pathogens responsible for development of the soft rot, samples were collected from storage roots with soft rot from intentionally flooded fields and decayed tissue was streaked on plates of nutrient dextrose agar plus 0.05% cysteine and incubated anaerobically. Two distinct groups of Gram positive strict anaerobic bacteria were re-isolated from rotting storage roots. Endospores were observed in all …

Proteomics-Based Study Of Host-Fungus Interaction Between Soybean And Phakopsora Pachyrhizi Using Recombinant Inbred Line (Ril) Derived Sister Lines, Mala Ganiger

LSU Master's Theses

Phakopsora pachyrhizi, the causal agent of Asian soybean rust (ASR), has the potential to cause severe yield losses as all United States commercial soybean varieties are susceptible. In this study, 10 soybean recombinant inbred line (RIL) derived sibling lines of two populations (RN06-32-2 and RN06-16-1) were evaluated for differences in response to infection by P. pachyrhizi. These lines, which had previously shown differential responses to Florida soybean rust isolates, were evaluated using Louisiana soybean rust isolates under both detached leaf assay and greenhouse in planta inoculation conditions. Sibling lines showed significant differences in response to P. pachyrhizi infection under both …

The Influence Of Commerce Silt Loam Soil Texture On Reproduction And Pathogenicity Of Rotylenchulus Reniformis On Cotton, Déborah Magalhães Xavier

LSU Master's Theses

Greenhouse and microplot studies were conducted to evaluate the influence of soil texture on reproduction and pathogenicity of Rotylenchulus reniformis (reniform nematode) on cotton. A 45 day duration greenhouse experiment confirmed the pathogenicity of an isolate of R. reniformis from Avoyelles Parish on Stoneville LA887 cotton. A series of greenhouse experiments were conducted with three geographic isolates of R. reniformis (identified as Avoyelles, Evangeline, and Rapides to indicate the Parish of origin) on Stoneville LA887, Stoneville 5288B2F, and Phytogen 375WF cotton growing in soils with varying textures for 60 days. Soil types with sand, silt, and clay contents ranging from …

Integrated Functional Anlaysis Of Quorum-Sensing In The Rice Pathogenic Bacterium Burkholderia Glumae, Ruoxi Chen

LSU Doctoral Dissertations

Quorum sensing (QS) is a cell-to-cell communication mechanism that allows bacterial cells to collectively behave like a multicellular organism. It regulates the expression of toxoflavin, one of the major virulence factors of the rice pathogen, Burkholderia glumae. The QS system of B. glumae is mediated by the core genes, tofI and tofR. N-octanoyl-L-homoserine lactone, the primary QS signal molecule of B. glumae, is synthesized by tofI and binds to the cognate receptor tofR at the quorum point. However, tofI and tofR null mutants produce toxoflavin in certain growth conditions, indicating the presence of tofI- and tofR-independent pathways for toxoflavin production. …

A Novel System To Study Seed Recalcitrance And Dormancy - Comparative Proteomics Between Two Spartina Species, Yi Wang

LSU Doctoral Dissertations

Spartina alterniflora is a dominant salt marsh cordgrass along the U.S. Atlantic and Gulf coasts, and the species is widely used for wetland restoration in Louisiana. S. alterniflora seeds are shed dormant from the mother plant. However, long-term preservation of S. alterniflora seeds is challenging because the seeds are recalcitrant, losing viability when dried below 45% water content. In this dissertation, I investigated the following three aspects of S. alterniflora: recalcitrance, dormancy release by cold stratification and the effect of drying temperature on the critical water content. Comparative proteomics between S. alterniflora and orthodox, desiccation tolerant Spartina pectinata seeds identified …

Chlorophyll Fluorescence And Thermal Stress In Archaias Angulatus (Class Foraminifera), Heidi M. Toomey

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Benthic foraminifers that host algal symbionts are similar to corals in that they rely on their algal endosymbionts for their energy needs, calcify prolifically, and are sensitive to changes in environmental conditions. They are abundant in the benthos of coastal coral-reef areas and are found throughout the tropical and subtropical regions. Pulse Amplitude Modulated (PAM) chlorophyll fluorometry and chlorophyll a extraction techniques were used to quantify and compare the photosynthetic responses of the benthic foraminiferal, Archaias angulatus and their isolated endosymbionts, Chlamydomonas hedleyi, to short-term changes in temperature. Maximum quantum efficiency (F_v/F_m) and rapid …

The Effect Of Temperature On Seed Quality And Quantity In Crosses Between European (Populus Tremula) And Hybrid Aspens (P. Tremula X P. Tremuloides), L. Koviuranta, T. Latva-Karjanmaa, P. Pulkkinen

Aspen Bibliography

Hybrid aspen (Populus tremula L. Populus tremuloides Michx.) plantations are expanding in Fennoscandia and the Baltic countries; however, the possible effects of plantations on the native European aspen (P. tremula) and the level of gene flow between European and hybrid aspen have not been investigated. We studied seed quantity and quality in intraspecific and interspecific crosses of the European and hybrid aspens over a two year period. In order to study whether elevated temperatures due to climate change would benefit the species differently, we performed the crosses in different temperatures. In both years, interspecific crosses produced more seeds with higher …

Bmp Planning To Address Urban Runoff: Plaster Creek Watershed Sustain Pilot, Tetra Tech, Inc.

Watershed Reports

The Plaster Creek watershed is tributary to the Grand River in west Michigan. Plaster Creek is one of several impaired streams in this area, located in metropolitan Grand Rapids. There are several listed impairments for the Creek including 1) other indigenous aquatic life and wildlife, 2) warm water fishery, and 3) total and partial body contact recreation. Total maximum daily load (TMDL) studies were completed in 2002 for biota and E. coli to address the listed impairments. In 2008, a watershed management plan (WMP) was developed which outlines an implementation plan for water quality improvement in the watershed. This pilot …

Polyphenoloxidase And Perioxidase Activity During Open Air Ripening Storage Of Pineapple (Ananas Comosus L.), Mango (Mangifera Indica) And Papaya (Carica Papaya) Fruits Grown In Dar Es Salaam, Tanzania, Oc Othman

Tanzania Journal of Science

Polyphenoloxidase (PPO) and perioxidase (POD), the enzymes responsible for causing browning and change in texture and flavor of fruits and vegetables, were extracted and measured in harvested mature pineapple (Ananas comosus L.), mango (Mangifera indica) (Viringe and Dodo varieties) and papaya (Carica papaya) fruits during off vine, open air, room temperature ripening storage. The initial (at harvest) average PPO activity values in Δ Optical Density (OD) per minute per cm3 of enzyme solution were 0.00074, 0.00083 and 0.0010 for early, mid and late season pineapple fruits respectively. The initial average PPO activity values in ΔOD/min/ cm3 of enzyme solution were …

Heavy Metal Content In Some Commonly Consumed Vegetables From Kariakoo Market, Dar Es Salaam, Tanzania, Eb Mubofu

Tanzania Journal of Science

This work reports on the levels of cadmium, lead, copper, manganese and zinc in 174 samples from eight varieties of green vegetables (amaranth, Chinese cabbage, cowpea leaves, green pepper, leafy cabbage, lettuce, okra and pumpkin leaf) commonly grown in Dar es Salaam and marketed at Kariakoo, Tanzania. The amount of cadmium and lead ranged between 3-42 and 11-60 μg/kg respectively. The amount of copper, manganese and zinc ranged between 0.35 - 2.12, 1.01 - 7.57, and 1.19 - 6.33 μg/kg respectively. On average consumption pattern, the contribution of these vegetables to the recommended daily intakes (RDI) for cadmium and lead …

Electrochemical Behaviour Of Environmentally Friendly Inhibitor Of Aloe Secundiflora Extract In Corrosion Control Of Carbon Steel In Soft Water Media, J Mutasingwa, J Buchweishaija, Jeg Mdoe

Tanzania Journal of Science

Electrochemical behaviour of Aloe secundiflora on carbon steel corrosion control in neutral and aerated soft water solutions have been investigated using electrochemical impedance spectroscopy and Tafel polarization techniques. The investigation was performed at different inhibitor concentrations under static and dynamic conditions using a Rotating Disk Electrode (RDE). The impedance and polarization results revealed that A. Secundiflora could be a potential corrosion inhibitor for carbon steel undergoing corrosion in fresh water medium. It was found that Aloe secundiflora reduces the electrochemical processes taking place on carbon steel undergoing corrosion. The inhibitor efficiency increases with increase in A. Secundiflora concentration and gradually …

Effects Of Temperature On The Physicochemical Properties Of Traditionally Processed Vegetable Oils And Their Blends, Fn Ngassapa, Ss Nyandoroa, Tr Mwaisaka

Tanzania Journal of Science

The quality and stability of traditionally processed palm, sunflower and sesame oils and their blends as affected by temperature were evaluated by analyzing their physicochemical properties which included acid, saponification, peroxide, iodine values and refractive indices.. The initial quality of traditionally processed oils studied was found to be within the specifications of TZS and Codex requirements. The results further indicate that the quality of both unblended and blended traditionally processed vegetable oils deteriorate significantly at elevated temperatures (p < 0.05). However, the blends showed relatively better quality and stability than the unblended ones whereby the formulations of palm oil (PO) and sesame oil (SSO) appeared to be more stable than those of PO and sunflower oil (SO). This work suggests that blending of traditionally processed PO, SO and SSO improves the quality and stability of these oils.

Activity Of Enzyme Trypsin Immobilized Onto Macroporous Poly(Epoxy-Acrylamide) Cryogel, W Johnson, Ymm Makame

Tanzania Journal of Science

Bovine pancreatic trypsin has been covalently immobilized onto monolithic polyacrylamide cryogels with epoxy functionality (Epoxy-MPAAGs). The covalent immobilization of trypsin onto Epoxy-MPAAGs was achieved through incorporation of ethylenediamine-glutaraldehyde spacers on the cryogels’ surfaces. The immobilization yield and the influence of pH and temperature conditions on the activity of the immobilized trypsin were assessed by using the hydrolysis of a low molecular weight substrate, N-α-benzoyl-D, L-arginine-p-nitroanilide (BAPNA). Optimal pH and temperature values of 9 and 50 °C, respectively, were established for both the immobilized trypsin and the free trypsin. Activity measurements from the native trypsin before immobilization and the residual free …

Local Filters Limit Species Diversity, But Species Pools Determine Composition, Tara K. Rajaniemi, Deborah E. Goldberg, Roy Turkington, Andrew R. Dyer

Faculty Publications

In a series of three experiments, we tested for effects of species pools, resource stress, and species interactions on four aspects of community structure: species richness, evenness, species composition, and functional group composition. We also examined whether the impacts of species interactions on the community varied with resource availability or species pool. Communities of sand dune annuals grew from seed bank samples collected from two sites in three different years, so that the species pool differed at two levels: the source site and the year of seed bank collection. Communities experienced one of three irrigation treatments and a range of …

Human Calmodulin Methyltransferase: Expression, Activity On Calmodulin, And Hsp90 Dependence, Sophia Magen, Roberta Magnani, Sitvanit Haziza, Eli Hershkovitz, Robert Houtz, Franca Cambi, Ruti Parvari

Horticulture Faculty Publications

Deletion of the first exon of calmodulin-lysine N-methyltransferase (CaM KMT, previously C2orf34) has been reported in two multigene deletion syndromes, but additional studies on the gene have not been reported. Here we show that in the cells from 2p21 deletion patients the loss of CaM KMT expression results in accumulation of hypomethylated calmodulin compared to normal controls, suggesting that CaM KMT is essential for calmodulin methylation and there are no compensatory mechanisms for CaM methylation in humans. We have further studied the expression of this gene at the transcript and protein levels. We have identified 2 additional …

Traversing Swanton Road, 10th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …

Characterization Of Chemotype And Aggressiveness Of Nebraska Isolates Of Fusarium Graminearum, Anita Panthi

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

Fusarium head blight (FHB), caused mainly by Fusarium graminearum, is a devastating disease of wheat and other small grain cereals. FHB lowers grain yield and quality and contaminates grain with mycotoxins, predominantly deoxynivalenol (DON) and its acetylated derivatives 3-ADON and 15-ADON. Forty one Fusarium isolates collected from grain elevators and wheat fields in Nebraska in 2009 and 2010 were sequenced for molecular identification. Forty isolates were identified as F. graminearum and one isolate was identified as F. culmorum. Seventy seven F. graminearum isolates collected from grain elevators and wheat fields in Nebraska from 2007 to 2010 were tested …

Major Fusarium Diseases On Corn, Wheat, And Soybeans In Nebraska, Bo Liu, Loren J. Giesler, Tamra A. Jackson-Ziems, Stephen N. Wegulo, Robert M. Harveson, Kevin A. Korus, Robert N. Klein

Department of Plant Pathology: Faculty Publications

Fusarium species are associated with diseases of corn, wheat, and soybean, causing significant yield loss in Nebraska. Some produce mycotoxins that are harmful to humans and animals.

Resistance Screening And Control Options For Glyphosate-Resistant Palmer Amaranth (Amaranthus Palmeri) In Cotton (Gossypium Hirsutum), Ryan Christopher Doherty

Graduate Theses and Dissertations

In the mid-2000's, glyphosate-resistant Palmer amaranth biotypes began to emerge in many southern states. In 2006, glyphosate-resistant Palmer amaranth was identified in a field in Mississippi County, Arkansas. A greenhouse experiment was conducted in 2008 to screen Palmer amaranth accessions, collected in this survey, for glyphosate resistance. Inflorescence were collected from a total of 276 plants from fields were glyphosate failure occurred, representing 74 accessions in 14 counties, including Clay, Craighead, Crittenden, Greene, Jackson, Jefferson, Lawrence, Lee, Mississippi, Phillips, Poinsett, Randolph, St. Francis, and White Counties. Eight of the 74 accessions did not produce viable seed. In the greenhouse, 32 …

Updated Manuscript Submission Guidelines For The Prairie Naturalist, Christopher N. Jacques, Troy W. Grovenburg, Jonathan A. Jenks

The Prairie Naturalist

These guidelines present updated policies and procedures for submitting scientific manuscripts for consideration for publication in The Prairie Naturalist (PNAT). These guidelines incorporate substantial changes that have occurred following a change in Editorial staff during January 2009, and update the online “Suggestions for Contributors” guidelines provided on the PNAT website (http://www.sdstate.edu/nrm/organizations/gpnss/tpn/index.cfm); these instructions supersede all previous guidelines.

Tables and appendices are included for common word expressions with superfluous wording (Table 1), examples of correct format and style guidelines for tables accompanying manuscripts (Table 2, Appendix A), guidance in properly preparing Research notes (Appendix B) and citing literature (Appendix …

Terrestrial And Semi-Aquatic Vertebrates In Diets Of Largemouth Bass In Central Nebraska, Seth A. Lundgren, Keith Geluso, Casey W. Schoenebeck

The Prairie Naturalist

Largemouth bass (Micropterus salmoides) are opportunistic predators often feeding in littoral areas during crepuscular periods (Snow 1971). Adult largemouth bass primarily are piscivorous, whereas juveniles generally consume zooplankton, insects, and small fish (Zweiacker and Summerfelt 1974, Carlander 1977, Post 2003). Age-0 largemouth bass generally consume zooplankton and macroinvertebrates (Keast and Webb 1966, Keast 1985, Phillips et al. 1995, Post 2003) and shift to piscivory at 50-70 mm in length (Phillips et al. 1995, Olson 1996, Post 2003). Few studies have documented detailed accounts of other vertebrates besides fish in diets of largemouth bass, which include amphibians (Hodgson and …

The Incentive And Benefits To Publish Research, Christopher N. Jacques

The Prairie Naturalist

In the northern Great Plains, December is a month marked by the end of the year and a period of much-needed rest and relaxation during the start of the winter season. Landscapes will soon be blanketed with snow and local floral and faunal species have adapted to the ensuing winter months ahead. Winter is a season that reminds you who you live in the Plains states, and as such, should expect bitter cold temperatures, strong winds, and blowing and drifting snow. However, December is a great time to enjoy the outdoors across the Great Plains, a time to reflect on …

Plant Community Composition And Structure Monitoring For Scotts Bluff National Monument, 2012 Annual Report, Isabel W. Ashton, Michael Prowatzke, Stephen K. Wilson

United States National Park Service: Publications

Executive Summary

the last remnants of native mixed-grass prairie in the region. The Northern Great Plains Inventory & Monitoring Network (NGPN) surveyed 8 long-term monitoring plots in Scotts Bluff National Monument in 2012 as part of an effort to better understand the condition of plant communities in the park. We measured plant diversity and cover, estimated tree and shrub density, looked for the presence of exotic species that are of concern to park management, and evaluated the amount of human and natural disturbance at all plots. This effort was the second year in a multiple-year venture to document the current …

Foliar Applied Urea Nitrogen Metabolism In Warm-Season Turfgrass Under Salinity Stress, Nicholas Menchyk

All Dissertations

The most widely used foliar nitrogen (N) source for warm-season turfgrass and agriculture is urea [(NH2)2CO], due to its low cost, high percentage of N (46% by mass), and completely soluble nature. Since urea is a soluble N source, it is commonly utilized as a foliar N source when tank mixed with pesticides in warm-season turfgrass management. The N in urea is not directly available to the plant until it is hydrolyzed into ammonia by the enzyme urease in the cytosol. Urease is a nickel (Ni2+) dependant enzyme that is ubiquitous in plants. Its main biochemical function is the hydrolysis …

Effects Of Slash Pile Burning After Restoring Conifer- Encroached Aspen, Christa M. Dagley, John-Pascal Berrill, Stephanie Coppeto, Kyle Jacobson

Aspen Bibliography

Removal of conifers encroaching aspen stands has been advocated and is being practiced in the Lake Tahoe Basin (EIP Project #10080: Aspen Community Restoration Projects). In remote and roadless areas, thinning of conifers is generating large volumes of wood and pile burning is currently being implemented to handle this biomass on site. However, the effects of pile burning on aspen are unknown, and there is an urgent need for guidelines to support design of thinning treatment prescriptions; specifically burn pile size and safe distances from live aspen trees of any size to prevent injury.

Southeast South Dakota Experiment Farm Annual Progress Report, 2012, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This document highlights 23 crop and livestock research and demonstration reports from projects conducted at Southeast Research Farm in 2012. It is published by the South Dakota Agricultural Experiment Station and Cooperative Extension Service at South Dakota State University in cooperation with the Southeast South Dakota Experiment Farm Corporation. Reports in this document include information on: weather and climate data, corn production and performance, soybean research and planting, soil testing, alfalfa yield test, fertilizer testing, herbicide research, crop rotation, sorghum, small grains, swine research, and pest and weed control.