Digital Commons Network^™

Sirna-Directed Dna Methylation In Plants, Meng Xie, Bin Yu

Center for Plant Science Innovation: Faculty Publications

DNA cytosine methylationis an important epigenetic process that is correlated with transgene silencing, transposon suppression, and gene imprinting. In plants, small interfering RNAs (siRNAs) can trigger DNA methylation at loci containing their homolog sequences through a process called RNAdirected DNA methylation (RdDM). In canonical RdDM, 24 nucleotide (nt) siRNAs (ra-siRNAs) will be loaded into their effector protein called ARGONAUTE 4 (AGO4) and subsequently targeted to RdDM loci through base-pairing with the non-coding transcripts produced by DNA-directed RNA Polymerase V. Then, the AGO4-ra-siRNA will recruit the DNA methyltransferase to catalyze de novo DNA methylation. Recent studies also identified non-canonical RdDM pathways …

The Role Of Ethylene In Plant Responses To K+ Deficiency, Daniel P. Schachtman

Center for Plant Science Innovation: Faculty Publications

Potassium is an essential macronutrient that is involved in regulating turgor , in driving plant growth, and in modulating enzyme activation. The changes in root morphology, root function, as well as cellular and molecular responses to low potassium conditions have been studied in the model plant Arabidopsis and in other plant species. In Arabidopsis ethylene plays a key role in roots in the transduction of the low potassium signal, which results in altered root function and growth. The first clues regarding the role of ethylene were detected through transcriptional profiling experiments showing changes in the expression of genes related to …

Redirection Of Metabolic Flux For High Levels Of Omega-7 Monounsaturated Fatty Acid Accumulation In Camelina Seeds, Huu Tam Nguyen, Hyunwoo Park, Karen L. Koster, Rebecca E. Cahoon, Hanh Nguyen, John Shanklin, Thomas E. Clemente, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Seed oils enriched in omega-7 monounsaturated fatty acids, including palmitoleic acid (16:1Δ9) and cis-vaccenic acid (18:1Δ11), have nutraceutical and industrial value for polyethylene production and biofuels. Existing oilseed crops accumulate only small amounts (<2%) of these novel fatty acids in their seed oils. We demonstrate a strategy for enhanced production of omega-7 monounsaturated fatty acids in camelina (Camelina sativa) and soybean (Glycine max) that is dependent on redirection of metabolic flux from the typical Δ9 desaturation of stearoyl (18:0)-acyl carrier protein (ACP) to Δ9 desaturation of palmitoyl (16:0)-acyl carrier protein (ACP) and coenzyme A (CoA). This was achieved by seed-specific co-expression of a mutant Δ9-acyl-ACP and an acyl-CoA desaturase with high specificity for 16:0-ACP and CoA substrates, respectively. This strategy was most effective in camelina where seed oils with ~17% omega-7 monounsaturated fatty acids were obtained.

Further increases in omega-7 fatty acid accumulation to 60–65% of the total fatty acids in camelina seeds were achieved by inclusion of seed-specific suppression of 3-keto-acyl-ACP synthase II and the FatB 16:0-ACP thioesterase genes to increase substrate pool sizes of 16:0-ACP for the Δ9-acyl-ACP desaturase and by blocking C18 fatty acid elongation. Seeds from these lines also had total saturated fatty acids reduced to ~5% of …

Production Of High Levels Of Poly-3-Hydroxybutyrate In Plastids Of Camelina Sativa Seeds, Meghna R. Malik, Wenyu Tang, Nii Patterson, Jihong Tang, Rachel L. Wellinghoff, Mary L. Preuss, Claire Burkitt, Nirmala Sharma, Yuanyuan Ji, Joseph M. Jez, Oliver P. Peoples, Jan G. Jaworski, Edgar B. Cahoon, Kristi D. Snell

Center for Plant Science Innovation: Faculty Publications

Poly-3-hydroxybutyrate (PHB) production in plastids of Camelina sativa seeds was investigated by comparing levels of polymer produced upon transformation of plants with five different binary vectors containing combinations of five seed-specific promoters for expression of transgenes. Genes encoding PHB biosynthetic enzymes were modified at the N-terminus to encode a plastid targeting signal. PHB levels of up to 15% of the mature seed weight were measured in single sacrificed T1 seeds with a genetic construct containing the oleosin and glycinin promoters.

A more detailed analysis of the PHB production potential of two of the best performing binary vectors in a Camelina …

Variable Frequency Of Plastid Rna Editing Among Ferns And Repeated Loss Of Uridine-To-Cytidine Editing From Vascular Plants, Wenhu Guo, Felix Grewe, Jeffrey P. Mower

Center for Plant Science Innovation: Faculty Publications

The distinct distribution and abundance of C-to-U and U-to-C RNA editing among land plants suggest that these two processes originated and evolve independently, but the paucity of information from several key lineages limits our understanding of their evolution. To examine the evolutionary diversity of RNA editing among ferns, we sequenced the plastid transcriptomes from two early diverging species, Ophioglossum californicum and Psilotum nudum. Using a relaxed automated approach to minimize false negatives combined with manual inspection to eliminate false positives, we identified 297 C-to-U and three U-to-C edit sites in the O. californicum plastid transcriptome but only 27 C-to-U …

Toward Production Of Jet Fuel Functionality In Oilseeds: Identification Of Fatb Acyl-Acyl Carrier Protein Thioesterases And Evaluation Of Combinatorial Expression Strategies In Camelina Seeds, Hae Jin Kim, Jillian E. Silva, Hieu Sy Vu, Keithanne Mockaitis, Jeong-Won Nam, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Seeds of members of the genus Cuphea accumulate medium-chain fatty acids (MCFAs; 8:0–14:0). MCFA- and palmitic acid- (16:0) rich vegetable oils have received attention for jet fuel production, given their similarity in chain length to Jet A fuel hydrocarbons. Studies were conducted to test genes, including those from Cuphea, for their ability to confer jet fuel-type fatty acid accumulation in seed oil of the emerging biofuel crop Camelina sativa. Transcriptomes from Cuphea viscosissima and Cuphea pulcherrima developing seeds that accumulate >90% of C8 and C10 fatty acids revealed three FatB cDNAs (CpuFatB3, CvFatB1, and CpuFatB4) …

Microrna Biogenesis, Degradation And Activity In Plants, Meng Xie, Shuxin Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

microRNAs (miRNAs) are important regulators of gene expression. After excised from primary miRNA transcript by dicer-like1 (DCL1, an RNAse III enzyme), miRNAs bind and guide their effector protein named argonaute 1 (AGO1) to silence the expression of target RNAs containing their complementary sequences in plants. miRNA levels and activities are tightly controlled to ensure their functions in various biological processes such as development, metabolism and responses to abiotic and biotic stresses. Studies have identified many factors that involve in miRNA accumulation and activities. Characterization of these factors in turn greatly improves our understanding of the processes related to miRNAs. Here, …

Modifications Of Membrane Lipids In Response To Wounding Of Arabidopsis Thaliana Leaves, Hieu Sy Vu, Rebecca Roston, Sunitha Shiva, Manhoi Hur, Eve Syrkin Wurtele, Xuemin Wang, Jyoti Shah, Ruth Welti

Center for Plant Science Innovation: Faculty Publications

Mechanical wounding of Arabidopsis thaliana leaves results in modifications of most membrane lipids within 6 hours. Here, we discuss the lipid changes, their underlying biochemistry, and possible relationships among activated pathways. New evidence is presented supporting the role of the processive galactosylating enzyme SENSITIVE TO FREEZING2 in the wounding response.

Resistance Inducers And Plant Growth Regulators Show Only Limited And Transient Effects On Infection Rates, Growth Rates And Symptom Expression Of Apple Trees Infected With ‘Candidatus Phytoplasma Mali’, Silva Schmidt, Sanja Baric, Maya Massenz, Thomas Letschka, Valerie Vanas, Manfred Wolf, Christine Kerschbamer, Roland Zelger, Wolfgang Schweigkofler

Collected Faculty Scholarship

The effects of four commercially available bio-active compounds on the infection rates, symptom expression and growth rates of apple trees (Malus × domestica Borkh.) cv. Golden Delicious infected with ‘Candidatus Phytoplasma mali’ (the so-called Apple Proliferation phytoplasma or AP) were tested over a three-year period under controlled conditions. Post-infection treatments using Bion® (active ingredient: Acibenzolar-S-Methyl), Messenger® (Harpin protein), Regalis® (Prohexadione-Ca) and Dormex® (Cyanamide) had no significant effect on infection rates. Terminal growth of apple trees (grown as one-shoot pruned trees) was increased significantly by AP infection; Prohexadione-Ca was the only compound which had a significant (inhibiting) effect on the growth …

Loblolly Pine Growth Patterns On Reclaimed Mineland: Allometry, Biomass, And Volume, Jeremy Priest, Jeremy Stovall, Dean Coble, Brian Oswald, Hans Williams

Faculty Publications

Surface lignite coal mines in east Texas are commonly reforested using loblolly pine (Pinus taeda L.) following mining and reclamation activities. Due to the nature of such an extreme disturbance, altered growth patterns, growth rates, and productivity could be expected. We destructively sampled above- and belowground tissue to develop prediction equations specific to these sites. These prediction equations differed statistically from those found in the literature regarding unmined land. At the stand level, biomass and volume productivity appeared similar with young stands on reclaimed mineland performing slightly poorer than similarly managed unmined sites. Allometric partitioning of above- and belowground biomass …

Decomposition Of Three Common Moist-Soil Managed Wetland Plant Species, Daniel P. Collins, Warren C. Conway, Corey D. Mason, Jeffrey W. Gunnels

Faculty Publications

Moist-soil wetland management is used to precisely control delivery, duration, and timing of water addition to, and removal from, managed wetlands with targeted responses including germination and growth of desirable moist-soil plant species. Similarly, water delivery and removal drives decomposition of moist-soil plants as well as nutrient cycling within these systems, which is a key driver of productivity in such managed wetlands. Through deployment of litter bags, we examined rate of mass loss and decay coefficients of three locally abundant moist-soil annual species that are potentially valuable wintering-waterfowl food sources (nodding smartweed Persicaria lapathifolia, red-rooted flatnut sedge Cyperus erythrorhizos …

Biological Invasion Hotspots: A Trait-Based Perspective Reveals New Sub-Continental Patterns, Basil V. Iannone Iii, Kevin M. Potter, Qinfeng Guo, Andrew M. Liebhold, Bryan C. Pijanowski, Christopher M. Oswalt, Songlin Fei

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Invader traits (including plant growth form) may play an important, and perhaps overlooked, role in determining macroscale patterns of biological invasions and therefore warrant greater consideration in future investigations aimed at understanding these patterns. To assess this need, we used empirical data from a national-level survey of forest in the contiguous 48 states of the USA to identify geographic hotspots of forest plant invasion for three distinct invasion characteristics: invasive species richness, trait richness (defined as the number of the five following plant growth forms represented by the invasive plants present at a given location: forbs, grasses, shrubs, trees, and …

Riqueza De Helmintos Parásitos De Vertebrados Silvestres Del Estado De Hidalgo, México, Jorge Falcón-Ordaz, Scott Monks, Griselda Pulido-Flores, Luis García-Prieto, Georgina Lira-Guerrero

Estudios en Biodiversidad

La diversidad de helmintos parásitos de vertebrados del estado de Hidalgo, en México es poco conocida, a pesar de que su estudio se inició desde 1937. Asimismo, mucha de la información generada se ha publicado de manera dispersa, lo que dificulta aún más el establecer con precisión los valores actuales. Por ello, el objetivo de este trabajo fue determinar la riqueza de la fauna helmintológica asociada a vertebrados silvestres de Hidalgo. Para alcanzar dicho objetivo, se realizaron tres acciones: 1) recopilación bibliográfica retrospectiva de información; 2) consulta al acervo de diversas colecciones científicas nacionales e internacionales, e 3) incorporación de …

Calidad Del Agua En La Subcuenca Fluvial La Garita, Acapulco, Guerrero Por Monitoreo Con Insectos, Ana Yolanda Rosas-Acevedo, Audel Sánchez-Infante, José Luis Rosas-Acevedo

Estudios en Biodiversidad

La subcuenca La Garita (Región Hidrológica: Rh19Ac) ha sufrido la influencia del hombre, presenta escorrentía y momentos de estiaje, además de puntos aislados de contaminación por basura y descargas. Se realizó un monitoreo de insectos. Se exploró la posibilidad del uso del índice BMWP (Biological Monitoring Working Party), para arroyos y ríos, en la determinación de la calidad del agua y del ecosistema. En la parte alta o cabecera (BMWP=122) correspondió a excelente (ecosistema no impactado), en la zona media o de transferencia (BMWP-CR=118) fue buena (ecosistema no alterado de manera sensible) y en la parte baja o zona de …

Problemática De Contaminación En La Zona Agrícola De La Reserva De La Biosfera Barranca De Metztitlán, Hidalgo, México, Maritza López-Herrera, Leticia Romero-Bautista, Nahara Ayala-Sánchez, Irma E. Soria-Mercado, Amelia Portillo-López

Estudios en Biodiversidad

El presente trabajo se generó con el objetivo de dar a conocer la problemática que existe actualmente en una zona agrícola de gran importancia en el estado de Hidalgo, la “Vega de Metztitlán”, zona que forma parte de la Reserva de la Biosfera Barranca de Metztitlán, sitio declarado como reserva federal. El aporte de agua residual que se hace a través de los acuíferos que irrigan a la zona, ha generado que este sitio se vea expuesto a un gran riesgo de contaminación por metales pesados, hidrocarburos y demás contaminantes, los cuáles quedan biodisponibles para las plantas e indirectamente existe …

Los Hongos Poliporoides De La Subcuenca Del Río Metztitlán, Hidalgo, México, Leticia Romero-Bautista, Miguel Ángel Islas-Santillán, Maritza López-Herrera, Nahara Ayala-Sánchez, Irma Estela Soria-Mercado, Amelia Portillo-López, Tania Raymundo-Ojeda, Ricardo Valenzuela-Garza

Estudios en Biodiversidad

Los hongos poliporoides son excelentes descomponedores de la madera, aunque también hay ectomicorrizógenos; su importancia ecológica radica en que ayudan a mantener el equilibrio del ciclo del carbono en la naturaleza. Además presentan potencial biotecnológico dadas sus características nutrimentales, medicinales y degradadoras, por su capacidad de producir metabolitos secundarios y ser potencialmente cultivables. Se analiza la importancia del impacto ambiental en los ecosistemas de la subcuenca del río Metztitlán y la diversidad de este grupo de hongos asociados a ellos. Se revisaron ejemplares de herbario y se realizaron exploraciones micológicas en 13 comunidades vegetales de 11 municipios, y 37 localidades …

Algae Population Self-Replenishment, R. Corban Harwood

Faculty Publications - Department of Mathematics

This modeling scenario investigates the massive algal blooms that struck Lake Chapala, Mexico, in 1994. After reading a summary of news articles on the incident, students create an ODE system model from a verbal description of the factors, visualize this system using an executable Java applet (PPLANE) to predict overall behavior, and then analyze the nonlinear system using the Jacobian matrix, eigenvalues, phase plane, and feasibility conditions on parameters to fully describe the system behavior. Students are expected to be familiar with systems of differential equations, equilibria, jacobian matrices, and eigenvalues. Students will learn modeling from qualitative descriptions, nondimensionalization, applying …

Physical And Biological Effects Of Modified Polysorbate 20, Kevin Penfield, Bryan Young, Julie K. Young, Greg R. Kruger, Ryan Henry, Greg Lindner

West Central Research and Extension Center, North Platte

Three experimental polyoxyethylene sorbitan monolaurate derivatives were synthesized with molecular fingerprinting techniques applied to experimental materials, confirming the target compounds had been produced. Chemical property measurements were compiled that aligned with theoretical predictions and physical property measurements confirmed their intentional differences yielded the anticipated changes in surfactant behavior. Imidacloprid uptake data confirmed penetration of leaf cuticles was enhanced in the presence of polyoxyethylene sorbitan monolaurates with several experimental materials providing uptake equivalent to reference material. Select materials were included in field and greenhouse trials where observations included good biological response with a range of individual herbicides as well as improved …

Bacterial Wilt Of Dry-Edible Beans In The Central High Plains Of The U.S.: Past, Present, And Future, Robert M. Harveson, Howard F. Schwartz, Carlos A. Urrea, C. Dean Yonts

Panhandle Research and Extension Center

Bacterial wilt, caused by Curtobacterium flaccumfaciens pv. flaccumfaciens, was first recognized and described as a new dry bean disease near Redfield, South Dakota, after the 1921 growing season on the farm of the Office of Forage Investigations. The grower on this farm informed the federal investigators that he believed the disease to be the same one that killed 90% of his crop in 1920. For the 1921 season, he saved and planted the seed harvested from the previous year, resulting in an estimated 25% loss.

. . .

It is likely that wilt has “reappeared” and become widely established throughout …

Corn Residue Stocking Rate Affects Cattle Performance But Not Subsequent Grain Yield, L. A. Stalker, Humberto Blanco-Canqui, Jennifer A. Gigax, A. L. Mcgee, T. M. Shaver, S. J. Van Donk

West Central Research and Extension Center, North Platte

This study investigated effects of stocking rate on cattle performance, quality and quantity of corn residue, and impact of residue removal on grain yield for 5 yr at the University of Nebraska – Lincoln West Central Water Resources Field Laboratory near Brule, NE. Four removal treatments—1) no removal (control), 2) grazing at 2.5 animal unit month (AUM)/ ha, 3) grazing at 5.0 AUM/ha, and 4) baling—were applied to a center pivot–irrigated corn field (53 ha). The field was divided into eight 6.6-ha paddocks to which replicated treatments were assigned. Samples of residue were collected in October and March (before and …

Indicadores Bióticos De Calidad Ambiental Del Sur Del Estado De Tamaulipas, México. Caso De Estudio: Aves, René Bernardo Elías Cabrera-Cruz, Marco Antonio González-González, Julio César Rolón-Aguilar, Juan Carlos Gaytán-Oyarzún

Estudios en Biodiversidad

Este trabajo plantea una evaluación de prioridad biológica para la conservación de aves en la región sur de Tamaulipas, México. La metodología empleada fue la técnica Evaluación Rápida de Prioridad Biológica (ERPBIO). Esta permite determinar las principales zonas y grupos a conservar en flora y fauna para amplias zonas de estudio de manera rápida y precisa. La región sur de Tamaulipas comprende los municipios de Aldama, Altamira, Ciudad Madero, González y Tampico, con una superficie total de 8,969.05 km2. Dentro de los resultados obtenidos se elaboró una base de datos por municipio, encontrando una amplia cantidad de estudios con un …

La Biodiversidad De Los Hongos Ectomicorrízicos Y Su Importancia Para La Conservación Del Bosque En La Zona Poblana Del Parque Nacional Malintzi, Marco Antonio Marín-Castro, Virginia Silva-Díaz, Gladys Linares-Fleites, Ana María Castagnino, José Antonio Ticante-Roldán

Estudios en Biodiversidad

El Parque Nacional Malintzi comprende una superficie de 45,852 hectáreas, de las cuales corresponden 14,544 al estado de Puebla. El deterioro a su riqueza forestal tiene sus inicios desde la época colonial y hasta nuestros días la presión sobre los recursos de este parque nacional aumenta debido a que los pobladores de la zona, tradicionalmente ven en la montaña su fuente de sustento mediante la agricultura y la extracción de madera, que aunadas a la tala clandestina inmoderada, han influido drásticamente en la pérdida de biodiversidad y cambio de uso del suelo. Los objetivos de este trabajo fueron describir la …

Los Hongos Agaricales De Las Áreas De Encino Del Estado De Baja California, México, Nahara Ayala-Sánchez, Irma E. Soria-Mercado, Leticia Romero-Bautista, Maritza López-Herrera, Roxana Rico-Mora, Amelia Portillo-López

Estudios en Biodiversidad

Se realizó una recopilación de las especies de hongos del orden Agaricales (regionalmente conocido como “agaricoides”) de los bosques Quercus spp. de la península de Baja California (México), con la pretensión de contar con un panorama de la diversidad de este grupo taxonómico en el área, su distribución y dinámica dentro de estos ecosistemas y el papel ecológico de cada una de las especies citadas. Para la realización del presente estudio se reunió la información obtenida de las especies recolectadas de las diferentes localidades de las áreas de encino del estado de Baja California desde 1996 hasta el presente, ejemplares …

Los Agaves Mezcaleros Del Altiplano Potosino Y Zacatecano, Edgar León Esparza-Ibarra, Juan Violante-González, Scott Monks, Jorge Cadena Iñiguez, Cuauhtémoc Araujo-Andrade, Erich Dietmar Rössel-Kipping

Estudios en Biodiversidad

En el Altiplano Potosino y Zacatecano, los agaves mezcaleros son un componente socioeconómico importante ya que se pueden aprovechar desde el punto de vista económico, ya que tiene valor en el mercado para la producción de bebidas espirituosas como el mezcal; social, ya que su producción y modo de empleo genera jornales disminuyendo la migración; en la salud, por la producción de aguamiel, jarabes e inulinas como complemento nutricional; científico, por los aportes al conocimiento en la variación biológica de los agaves; debido a la diversidad y su plasticidad para enfrentar temperaturas extremas y sequías prolongadas, entre otros. Es por …

El Arbolado De Los Espacios Públicos Abiertos De La Zona De Monumentos Del Centro Histórico De La Ciudad De Puebla, Víctor Gutiérrez-Pacheco, Sonia Emilia Silva-Gómez, José Silvestre Toxtle-Tlamani, J. Santos Hernández-Zepeda

Estudios en Biodiversidad

Los conglomerados humanos, presentan espacios públicos abiertos (EPA´s), sobre todo arbolados, con funciones estéticas, sociales y ambientales. Se requiere información e indicadores con un enfoque ecológico, para que su atención descanse sobre una planeación documentada. Así, se pretende conocer la composición arbórea, su distribución y la biodiversidad en los Espacios Públicos Abiertos (EPA´s) de la Zona de Monumentos del Centro Histórico de la Ciudad de Puebla (ZMCHP). Por tanto, se contaron los especímenes, se ubicaron en los EPA´s y se identificaron. Para determinar la biodiversidad se aplicó el Índice de Diversidad de Shannon-Wiener (H´). En 25 EPA´s, se censaron 1,120 …

Belowground Bud Bank Of Bromus Inermis In Response To Mowing Frequency Over Two Growing Seasons, Denise Olson, Lan Xu, Arvid Boe, N. H. Troelstrup Jr.

Native Plant Focused Publications

Smooth bromegrass (Bromus inermis) is a Eurasian C3 perennial grass. It was introduced to North America in 1884 to control soil erosion and for pasture improvement, but has been outcompeting the native grasses of the northern Great Plains, decreasing diversity and altering habitats. Control of smooth bromegrass is a key to improve degraded native dominated grasslands. However, current practices have minimal or only short term effects due to extensive rhizome and tiller production by bromegrass. Even though vegetative reproduction via the belowground bud bank is the primary means for its invasiveness and persistence, the effect of management on its bud …

Using The Past To Restore The Future: Quantifying Historical Vegetation To Assist In Tidal Freshwater Wetland Restoration, Christopher D. Gatens

UROP Posters

Wetlands have been providing humans with critical natural ecosystem services throughout our time on Earth. Nevertheless, these invaluable ecosystems have been habitually altered as a cost of human progression. Two of the most common alterations to wetlands are damming and filling. Both occurred along Kimages Creek in Charles City County, VA during the 19^th and 20^th centuries. In 2010 the Lake Charles dam was partially removed, restoring the creek’s tidal communication with the James River. Upon the recession of the body of water, numerous woody stumps were revealed. We studied these stump remnants in an attempt to assess …

Two Hundred Years Of Forest Change: Effects Of Urbanization On Tree Species Composition And Structure, Rebecca W. Dolan

Scholarship and Professional Work - LAS

Despite their importance, the dynamics of urban floras are not well understood and quantitative historical data are rare. The current study used three data sets for trees in Indianapolis/Marion County, Indiana, U.S., to document change over 200 years to the original beech-maple forest and to examine future implications of contemporary tree planting efforts in light of these changes. Data on tree composition and size collected before significant settlement in the early 1800s are compared with recent surveys of trees in remnant natural areas and with trees found on city streets and rights-of-way. All the species recorded in historical surveys are …

Establishment Of Biotrophy By The Maize Anthracnose Pathogen Colletotrichum Graminicola: Use Of Bioinformatics And Transcriptomics To Address The Potential Roles Of Secretion, Stress Response, And Secreted Proteins, Ester Alvarenga Santos Buiate

Theses and Dissertations--Plant Pathology

Colletotrichum graminicola is a hemibiotrophic pathogen of maize that causes anthracnose leaf and stalk rot diseases. The pathogen penetrates the host and initially establishes an intracellular biotrophic infection, in which the hyphae are separated from the living host cell by a membrane that is elaborated by the host, apparently in response to pathogen signals. A nonpathogenic mutant (MT) of C. graminicola was generated that germinates and penetrates the host normally, but is incapable of establishing a normal biotrophic infection. The mutated gene is Cpr1, conserved in eukaryotes and predicted to encode a component of the signal peptidase complex. How …

Robert Folger Thorne—A Botanical Legacy

Aliso: A Journal of Systematic and Floristic Botany

Robert F. Thorne, distinguished botanist and former curator of the herbarium at Rancho Santa Ana Botanic Garden, left a rich botanical legacy. Contributions featured include a list of his articles appearing in Aliso: A Journal of Systematic and Evolutionary Botany, as well a list of plant taxa named for him.