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Articles 16741 - 16770 of 26310
Full-Text Articles in Entire DC Network
Chapter 17 - Rodents, Janice M. Glime
Chapter 17 - Rodents, Janice M. Glime
Bryophyte Ecology Volume 2: Bryological Interaction
Explore the contents of Bryophyte Ecology, Volume 2, Chapter 17 - Rodents by clicking the links above. For the Contents section of this ebook, as well as Volumes 1 through 5, please visit the Bryophyte Ecology Main Page.
Use CTRL+F to easily search within PDF files.
Ebook sponsored by Michigan Technological University and the International Association of Bryologists.
Foliar Fungicide Comparison For Southern Rust Management, Stay Green On Specific Leaves, And Yield In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems
Foliar Fungicide Comparison For Southern Rust Management, Stay Green On Specific Leaves, And Yield In Nebraska, 2015, J. D. Harbour, T. A. Jackson-Ziems
Department of Plant Pathology: Faculty Publications
The objective of the trial was to compare foliar fungicides for southern rust (SR) efficacy. Irrigated corn was grown based on Nebraska Extension irrigation recommendations at the South Central Ag Lab near Clay Center, NE. Soils were a silt loam with 6.7 pH and 1.8 % OM and the previous crop was soybean. Reduced tillage was performed to the field prior to planting. Corn (DKC 65-79 RIB, tolerant to gray leaf spot (GLS)) was planted at approximately 34,000 seed/A on 26 May. Five treatments were arranged in a randomized complete block design with six replications. . . . Phytotoxicity was …
Assessing How Disruption Of Methanogenic Communities And Their Syntrophic Relationships In Tidal Freshwater Marshes Via Saltwater Intrusion May Affect Ch4 Emissions, David J. Berrier, Scott C. Neubauer, Rima B. Franklin
Assessing How Disruption Of Methanogenic Communities And Their Syntrophic Relationships In Tidal Freshwater Marshes Via Saltwater Intrusion May Affect Ch4 Emissions, David J. Berrier, Scott C. Neubauer, Rima B. Franklin
Rice Rivers Center Research Symposium
Tidal freshwater wetlands (TFW), which lie at the interface of saltwater and freshwater ecosystems, are predicted to experience moderate salinity increases due to sea level rise. Increases in salinity generally suppress CH4 production, but it is uncertain to what extent elevated salinity will affect CH4 cycling in TFW. It is also unknown whether CH4 production will resume when freshwater conditions return. The ability to produce CH4 is limited to a monophyletic group of the Euryarchaeota phylum called methanogens (MG), who are limited to a small number of substrates (e.g., acetate, H2, and formate) produced from the breakdown of fermentation products. …
Chromosome-End Knockoff Strategy To Reshape Alkaloid Profiles Of A Fungal Endophyte, Simona Florea, Timothy D. Phillips, Daniel G. Panaccione, Mark L. Farman, Christopher L. Schardl
Chromosome-End Knockoff Strategy To Reshape Alkaloid Profiles Of A Fungal Endophyte, Simona Florea, Timothy D. Phillips, Daniel G. Panaccione, Mark L. Farman, Christopher L. Schardl
Faculty & Staff Scholarship
Molecular genetic techniques to precisely eliminate genes in asexual filamentous fungi require the introduction of a marker gene into the target genome. We developed a novel strategy to eliminate genes or gene clusters located in subterminal regions of chromosomes, and then eliminate the marker gene and vector backbone used in the transformation procedure. Because many toxin gene clusters are subterminal, this method is particularly suited to generating nontoxic fungal strains. We tested this technique on Epichloë coenophiala, a seed-transmissible symbiotic fungus (endophyte) of the important forage grass, tall fescue (Lolium arundinaceum). The endophyte is necessary for maximal productivity and sustainability …
Data Supporting The Nuclear Phylogenomics Of The Palm Subfamily Arecoideae (Arecaceae), Jason R. Comer, Wendy B. Zomlefer, Craig F. Barrett, Dennis Wm. Stevenson, Karolina Heyduk, James H. Leebens-Mack
Data Supporting The Nuclear Phylogenomics Of The Palm Subfamily Arecoideae (Arecaceae), Jason R. Comer, Wendy B. Zomlefer, Craig F. Barrett, Dennis Wm. Stevenson, Karolina Heyduk, James H. Leebens-Mack
Faculty & Staff Scholarship
This data article provides data and supplemental materials refer-enced in“Nuclear phylogenomics of the palm subfamily Arecoi-deae (Arecaceae)”(Comer et al., 2016)[1]. Raw sequence readsgenerated for this study are available through the Sequence ReadArchive (SRA Study Accession: SRP061467). An aligned super-matrix of 168 nuclear genes for 35 taxa (34 palms and one out-group taxon) is provided. Also provided are individual maximumlikelihood gene trees used for the coalescent based analyses, out-put from the maximum parsimony analyses, and twofigures.
Waracs: Wrappers To Automate The Reconstruction Of Ancestral Character States, Michael Gruenstaeudl
Waracs: Wrappers To Automate The Reconstruction Of Ancestral Character States, Michael Gruenstaeudl
Biological Sciences Faculty Publications
Premise of the study: Reconstructions of ancestral character states are among the most widely used analyses for evaluating the morphological, cytological, or ecological evolution of an organismic lineage. The software application Mesquite remains the most popular application for such reconstructions among plant scientists, even though its support for automating complex analyses is limited. A software tool is needed that automates the reconstruction and visualization of ancestral character states with Mesquite and similar applications. Methods and Results: A set of command line–based Python scripts was developed that (a) communicates standardized input to and output from the software applications Mesquite, BayesTraits, and …
Annotated Checklist Of The Vascular Plant Species Observed At The Gordon Natural Area (West Chester University, Pa) - Version 10, Nur Ritter
Gordon Natural Area Baseline Plant Survey Documents
Information from previous versions of floral list are included in latest version.
Effects Of Sublethal Fungicides On Mutation Rates And Genomic Variation In Fungal Plant Pathogen, Sclerotinia Sclerotiorum, B. Sajeewa Amaradasa, Sydney E. Everhart
Effects Of Sublethal Fungicides On Mutation Rates And Genomic Variation In Fungal Plant Pathogen, Sclerotinia Sclerotiorum, B. Sajeewa Amaradasa, Sydney E. Everhart
Department of Plant Pathology: Faculty Publications
Pathogen exposure to sublethal doses of fungicides may result in mutations that may represent an important and largely overlooked mechanism of introducing new genetic variation into strictly clonal populations, including acquisition of fungicide resistance. We tested this hypothesis using the clonal plant pathogen, Sclerotinia sclerotiorum. Nine susceptible isolates were exposed independently to five commercial fungicides with different modes of action: boscalid (respiration inhibitor), iprodione (unclear mode of action), thiophanate methyl (inhibition of microtubulin synthesis) and azoxystrobin and pyraclostrobin (quinone outside inhibitors). Mycelium of each isolate was inoculated onto a fungicide gradient and sub-cultured from the 50±100% inhibition zone for 12 …
Global Synthesis Of The Classifications, Distributions, Benefits And Issues Of Terracing, Wei Wei, Die Chen, Lixin Wang, Stefani Daryanto, Liding Chen, Yang Yu, Yonglong Lu, Ge Sun, Tianjiao Feng
Global Synthesis Of The Classifications, Distributions, Benefits And Issues Of Terracing, Wei Wei, Die Chen, Lixin Wang, Stefani Daryanto, Liding Chen, Yang Yu, Yonglong Lu, Ge Sun, Tianjiao Feng
United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications
For thousands of years, humans have created different types of terraces in different sloping conditions, meant to mitigate flood risks, reduce soil erosion and conserve water. These anthropogenic landscapes can be found in tropical and subtropical rainforests, deserts, and arid and semiarid mountains across the globe. Despite the long history, the roles of and the mechanisms by which terracing improves ecosystem services (ESs) remain poorly understood. Using literature synthesis and quantitative analysis, the worldwide types, distributions, major benefits and issues of terracing are presented in this review. A key terracing indicator, defined as the ratio of different ESs under terraced …
Towards An Open Grapevine Information System, A-F Adam-Blondon, M. Alaux, C. Pommier, D. Cantu, Z-M Cheng, Gr Cramer, C. Davies, S. Delrot, L. Deluc, G. Di Gaspero, J. Grimplet, A. Fennell Dr, Et. Al
Towards An Open Grapevine Information System, A-F Adam-Blondon, M. Alaux, C. Pommier, D. Cantu, Z-M Cheng, Gr Cramer, C. Davies, S. Delrot, L. Deluc, G. Di Gaspero, J. Grimplet, A. Fennell Dr, Et. Al
Agronomy, Horticulture and Plant Science Faculty Publications
Viticulture, like other fields of agriculture, is currently facing important challenges that will be addressed only through sustained, dedicated and coordinated research. Although the methods used in biology have evolved tremendously in recent years and now involve the routine production of large data sets of varied nature, in many domains of study, including grapevine research, there is a need to improve the findability, accessibility, interoperability and reusability (FAIR-ness) of these data. Considering the heterogeneous nature of the data produced, the transnational nature of the scientific community and the experience gained elsewhere, we have formed an open working group, in the …
Chapter 5 - Ecophysiology Of Development, Janice M. Glime
Chapter 5 - Ecophysiology Of Development, Janice M. Glime
Bryophyte Ecology Volume 1: Physiological Ecology
Explore the contents of Bryophyte Ecology, Volume 1, Chapter 5 - Ecophysiology of Development by clicking the links above. For the Contents section of this ebook, as well as Volumes 1 through 5, please visit the Bryophyte Ecology Main Page.
Use CTRL+F to easily search within PDF files.
Ebook sponsored by Michigan Technological University and the International Association of Bryologists.
A Comparative Study Of The Flora And Soils Of Great Duck And Little Duck Islands, Maine, Usa, Luka Negoita, Matthew Dickinson, Glen Mittelhauser, Nishanta Rajakaruna
A Comparative Study Of The Flora And Soils Of Great Duck And Little Duck Islands, Maine, Usa, Luka Negoita, Matthew Dickinson, Glen Mittelhauser, Nishanta Rajakaruna
Faculty Publications, Biological Sciences
Strong environmental gradients and varied land-use practices have generated a mosaic of habitats harboring distinct plant communities on islands on the coast of Maine. Botanical studies of Maine's islands, however, are generally limited in number and scope. Baseline studies of Maine's islands are necessary for assessing vegetation dynamics and changes in habitat conditions in relation to environmental impacts imposed by climate change, rising sea levels, invasive species, pests and pathogens, introduced herbivores, and human disturbance. We conducted a survey of the vascular plants and soils of forest, field, and ocean-side communities of Great Duck and Little Duck Islands, ME. These …
Limestone Flora Of The Simonton Corner Quarry Preserve, Rockport, Maine, Usa, Margaret Stern, Ian Medeiros, Luka Negoita, Nishanta Rajakaruna
Limestone Flora Of The Simonton Corner Quarry Preserve, Rockport, Maine, Usa, Margaret Stern, Ian Medeiros, Luka Negoita, Nishanta Rajakaruna
Faculty Publications, Biological Sciences
Limestone is a distinctive substrate that has significant effects on soils and plants. The present study characterizes the diversity of vascular plants, bryophytes, and lichens at the Simonton Corner Quarry Preserve, an abandoned limestone quarry in Rockport, Maine, USA, which was in operation in the late 1800s. We document vascular plant diversity and associated edaphic features (i.e., soil pH and elemental chemistry) using 30 5×5 meter plots spread throughout the site. For vascular plants, 114 species in 96 genera and 50 families were observed; few of these species are known to prefer calcareous environments, and 38% are nonnative. Conversely, the …
Igrow Corn: Best Management Practices, David E. Clay, Gregg G. Carlson, Sharon A. Clay, Emmanuel Byamukama
Igrow Corn: Best Management Practices, David E. Clay, Gregg G. Carlson, Sharon A. Clay, Emmanuel Byamukama
Agronomy, Horticulture, and Plant Science Books
No abstract provided.
Ua24 Mcicsl Newsletter - January 2016, Shannon R. Trimboli Education Coordinator
Ua24 Mcicsl Newsletter - January 2016, Shannon R. Trimboli Education Coordinator
MCICSL Newsletter
This issue includes the following:
Information about the upcoming 11th Research Symposium
New Stonefly species
Winter Bat Monitoring
as well as research highlights and education highlights
The Ecology And Evolution Of Rare, Soil Specialist Astragalus Plants In The Arid Western U.S., Joseph M. Statwick
The Ecology And Evolution Of Rare, Soil Specialist Astragalus Plants In The Arid Western U.S., Joseph M. Statwick
Electronic Theses and Dissertations
Organisms that specialize in uncommon habitats are, by their very nature, inherently uncommon. Specialization has its advantages, namely reduced competition and predation, but it also incurs costs. Specialists often have small population sizes, narrow ranges, and fragmented habitat, all of which engender negative consequences on an evolutionary timescale. Herein, I examine benefits and costs of specialization in selenium-hyperaccumulating plants in the genus Astragalus (Fabaceae). These plants are disproportionately likely to be rare and of conservation concern. Thus, I optimized germination pretreatments for Astragalus species such that seed loss can be minimized during ex situ cultivation, and found that physical scarification …
08. Strengthening Karner Blue Butterfly Metapopulations, John Shillinglaw
08. Strengthening Karner Blue Butterfly Metapopulations, John Shillinglaw
North American Prairie Conference Proceedings
Wild lupine (Lupinus perennis) is the obligate host plant for larvae of the Karner blue butterfly (Lycaeides melissa samuelis). The purpose of this study was to determine whether planting wild lupine in dry prairie sites (areas previously devoid of wild lupine but having appropriate habitat structure for its growth) would result in colonization from existing populations of Karner blue butterflies over time, thereby adding to metapopulation stability. The newly planted sites were on private and public land and ranged in area from 0.81 to 8.1 ha (from 2 to 20 acres). They were located within about 3.2 km (2 mi) …
15. The Conservation Significance Of Prairie Remnants In Missouri, Bruce Schuette
15. The Conservation Significance Of Prairie Remnants In Missouri, Bruce Schuette
North American Prairie Conference Proceedings
How to conserve the great biodiversity of the prairie resource is a question of continuous debate. In Missouri the estimated 6,070,284 ha (15 million acres) of prairie at the time of Euro-American settlement has been reduced to an estimated 20,234–24,281 ha (50,000–60,000 acres) today, with 8,093 ha (20,000 acres) in conservation ownership. This valuable resource is rare now and is continuing to disappear. The Missouri Prairie Foundation (MPF), a nongovernmental organization celebrating its 50th anniversary in 2016, has acquired and protects 22 tracts that cover more than 1,335 ha (3,300 acres), comprised mostly of original unplowed prairie. These parcels, though …
04. Population Trends Of Breeding Grassland Birds At Midewin National Tallgrass Prairie, 1985–2015, James R. Herkert, William D. Glass
04. Population Trends Of Breeding Grassland Birds At Midewin National Tallgrass Prairie, 1985–2015, James R. Herkert, William D. Glass
North American Prairie Conference Proceedings
We use data from ongoing bird monitoring programs to assess long-term population trends at Midewin National Tallgrass Prairie in northeastern Illinois. Midewin is the nation’s first National Tallgrass Prairie and was established in 1996 on the site of the former Joliet Army Ammunition Plant. Annual bird monitoring began at the site in the early 1980s when it was discovered that the pastures and hayfields maintained by the Army contained significant grassland bird populations. Ninety-four species of breeding birds were recorded at the site between 2009 and 2015, including large populations of several grasslandobligate birds including dickcissel (Spiza americana), eastern meadowlark …
Ecosystem Services Of Woody Crop Production Systems, Ronald S. Zalesny Jr., John A. Stanturf, Emile S. Gardiner, James H. Perdue, Timothy M. Young, David R. Coyle, William L. Headlee, Gary S. Bañuelos, Amir Hass
Ecosystem Services Of Woody Crop Production Systems, Ronald S. Zalesny Jr., John A. Stanturf, Emile S. Gardiner, James H. Perdue, Timothy M. Young, David R. Coyle, William L. Headlee, Gary S. Bañuelos, Amir Hass
United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications
Short-rotation woody crops are an integral component of regional and national energy portfolios, as well as providing essential ecosystem services such as biomass supplies, carbon sinks, clean water, and healthy soils. We review recent USDA Forest Service Research and Development efforts from the USDA Biomass Research Centers on the provisioning of these ecosystem services from woody crop production systems. For biomass, we highlight productivity and yield potential, pest susceptibility, and bioenergy siting applications. We describe carbon storage in aboveground woody biomass and studies assessing the provision of clean and plentiful water. Soil protection and wildlife habitat are also mentioned, in …
16. A Comparison Of Soil Biodiversity In Restored Prairie Plots And Agricultural Fields At A Biomass Production Farm In Southeastern Minnesota, Bruno Borsari, Neal Mundahl, Malcolm Vidrine
16. A Comparison Of Soil Biodiversity In Restored Prairie Plots And Agricultural Fields At A Biomass Production Farm In Southeastern Minnesota, Bruno Borsari, Neal Mundahl, Malcolm Vidrine
North American Prairie Conference Proceedings
Communities of soil-dwelling organisms contribute to soil fertility and nutrient cycling, but conventional farming practices can disturb and reduce these communities. In southeastern Minnesota, some farmers are planting simplified prairie vegetation to produce biomass fuels. Our study was designed to assess the species abundance and diversity of soil invertebrates in plantings of mixed grasses only (MG) and mixed grasses with forbs (MGF) that were planted for use as a biomass fuel source on a farm in southeastern Minnesota in 2007. Abundance and diversity of soil invertebrates also were examined in soils of corn (Zea mays L.) fields grown on the …
17. Prairie Reverie: What Does The Word Prairie Mean In Postprairie Illinois?, Jack Shouba
17. Prairie Reverie: What Does The Word Prairie Mean In Postprairie Illinois?, Jack Shouba
North American Prairie Conference Proceedings
The meaning of the word ‘‘prairie’’ seems to have changed in recent years, as the Silicon Prairie replaced the Silphium prairie. There are bewildering uses of prairie that have nothing to do with native grasslands. The word is often used in a generic way, such as calling farmland, a vacant lot, or other open space a prairie. It is frequently used evocatively, as in Lakewood Prairie, a subdivision without a lake, a wood, or a prairie; a shopping center with no prairie for miles around is called Prairie View. Oxymorons like prairie golf or prairie fishing are common. Prairie reverie …
03. Population Demographics Of The Loggerhead Shrike: Insights Into The Species Decline From A Long-Term Study In The Midewin National Tallgrass Prairie, Amy A. Chabot, Fran Harty, James Herkert, William Glass
03. Population Demographics Of The Loggerhead Shrike: Insights Into The Species Decline From A Long-Term Study In The Midewin National Tallgrass Prairie, Amy A. Chabot, Fran Harty, James Herkert, William Glass
North American Prairie Conference Proceedings
We monitored a breeding population of loggerhead shrikes (Lanius ludovicianus) in the Midewin National Tallgrass Prairie from 2005 to 2016. Demographic data were compiled annually, including information on population size, age structure, and reproductive success. We banded adult shrikes, with some additional effort focused on nestlings (2005–2007) and independent hatch-year birds (2014–2016). We measured return rate as the number of birds that were previously banded. Site reuse rates were quantified as the use of a breeding territory in multiple years. Site fidelity, the use of a site by the same male or female in consecutive years, and dispersal distance of …
09. Matching Long-Term Fire Effects Research To Pressing Questions Facing Tallgrass Prairie Managers Across The Upper Midwest, Tom Bragg, Craig Maier, Yari Johnson
09. Matching Long-Term Fire Effects Research To Pressing Questions Facing Tallgrass Prairie Managers Across The Upper Midwest, Tom Bragg, Craig Maier, Yari Johnson
North American Prairie Conference Proceedings
The goal for this paper is to explore how a network of coordinated prescribed fire experiments could be developed and applied to tallgrass prairie management. In a 2011 survey conducted by the Tallgrass Prairie and Oak Savanna Fire Science Consortium in their region, 61% of 207 land managers indicated that their greatest need with respect to fire regimes was information on the outcome of variations in fire frequency and season, with information on these variables ranging from limited to completely lacking. Need for this kind of information was echoed during a breakout discussion session at the 2016 North American Prairie …
Agroforestry Horizons: Experiences And Reflections: Report-Out, Steve Rasmussen
Agroforestry Horizons: Experiences And Reflections: Report-Out, Steve Rasmussen
United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications
The USDA National Agroforestry Center (NAC) is charting a course for the next five years. As part of this process, NAC and the USDA Interagency Agroforestry Team hosted a meeting of leaders in the fields of agroforestry, agriculture, and forestry, from government, universities, and the private sector, to share experiences and insights on agroforestry opportunities and challenges. The meeting occurred on April 27th and 28th, 2016, in Washington, DC, and entailed presentations and discussions on current and potential roles of agroforestry in supporting water quality, soil health, economic diversification, food security, and pollinator/wildlife habitat. This report-out provides a summary of …
Screen Of Non-Annotated Small Secreted Proteins Of Pseudomonas Syringae Reveals A Virulence Factor That Inhibits Tomato Immune Proteases, Takayuki Shindo, Farnusch Kaschani, Fan Yang, Judit Kovács, Fang Tian, Jiorgos Kourelis, Tram Ngoc Hong, Tom Colby, Mohammed Shabab, Rohini Chawla, Selva Kumari, Muhammed Ilyas, Anja C. Hörger, James R. Alfano, Renier A. L. Van Der Hoorn
Screen Of Non-Annotated Small Secreted Proteins Of Pseudomonas Syringae Reveals A Virulence Factor That Inhibits Tomato Immune Proteases, Takayuki Shindo, Farnusch Kaschani, Fan Yang, Judit Kovács, Fang Tian, Jiorgos Kourelis, Tram Ngoc Hong, Tom Colby, Mohammed Shabab, Rohini Chawla, Selva Kumari, Muhammed Ilyas, Anja C. Hörger, James R. Alfano, Renier A. L. Van Der Hoorn
Center for Plant Science Innovation: Faculty Publications
Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. Here we identified 131 candidate small, secreted, non-annotated proteins from the PtoDC3000 genome, most of which are common to Pseudomonas species and potentially expressed during apoplastic colonization. We produced 43 of these proteins through a custom-made gateway-compatible expression system for extracellular bacterial proteins, and screened them for their ability to inhibit the secreted immune protease C14 of tomato using competitive activity-based protein profiling. This screen revealed C14-inhibiting protein-1 (Cip1), which contains …
Residues Responsible For The Selectivity Of Α-Conotoxins For Ac-Achbp Or Nachrs, Bo Lin, Shi-Hua Xiang, Mengsen Li
Residues Responsible For The Selectivity Of Α-Conotoxins For Ac-Achbp Or Nachrs, Bo Lin, Shi-Hua Xiang, Mengsen Li
Center for Plant Science Innovation: Faculty Publications
Nicotinic acetylcholine receptors (nAChRs) are targets for developing new drugs to treat severe pain, nicotine addiction, Alzheimer disease, epilepsy, etc. α-Conotoxins are biologically and chemically diverse. With 12–19 residues and two disulfides, they can be specifically selected for different nAChRs. Acetylcholine-binding proteins from Aplysia californica (Ac-AChBP) are homologous to the ligand-binding domains of nAChRs and pharmacologically similar. X-ray structures of the α-conotoxin in complex with Ac-AChBP in addition to computer modeling have helped to determine the binding site of the important residues of α-conotoxin and its affinity for nAChR subtypes. Here, we present the various α-conotoxin residues that are selective …
Screen Of Non-Annotated Small Secreted Proteins Of Pseudomonas Syringae Reveals A Virulence Factor That Inhibits Tomato Immune Proteases, Takayuki Shindo, Farnusch Kaschani, Fan Yang, Judit Kovács, Fang Tian, Jiorgos Kourelis, Tram Ngoc Hong, Tom Colby, Mohammed Shabab, Rohini Chawla, Selva Kumari, Muhammed Ilyas, Anja C. Hörger, James R. Alfano, Renier A. L. Van Der Hoorn
Screen Of Non-Annotated Small Secreted Proteins Of Pseudomonas Syringae Reveals A Virulence Factor That Inhibits Tomato Immune Proteases, Takayuki Shindo, Farnusch Kaschani, Fan Yang, Judit Kovács, Fang Tian, Jiorgos Kourelis, Tram Ngoc Hong, Tom Colby, Mohammed Shabab, Rohini Chawla, Selva Kumari, Muhammed Ilyas, Anja C. Hörger, James R. Alfano, Renier A. L. Van Der Hoorn
Center for Plant Science Innovation: Faculty Publications
Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. Here we identified 131 candidate small, secreted, non-annotated proteins from the PtoDC3000 genome, most of which are common to Pseudomonas species and potentially expressed during apoplastic colonization. We produced 43 of these proteins through a custom-made gateway-compatible expression system for extracellular bacterial proteins, and screened them for their ability to inhibit the secreted immune protease C14 of tomato using competitive activity-based protein profiling. This screen revealed C14-inhibiting protein-1 (Cip1), which contains motifs of …
Limited Mitogenomic Degradation In Response To A Parasitic Lifestyle In Orobanchaceae, Weishu Fan, Andan Zhu, Melisa Kozaczek, Neethu Shah, Natalia Pabón-Mora, Favio González, Jeffrey P. Mower
Limited Mitogenomic Degradation In Response To A Parasitic Lifestyle In Orobanchaceae, Weishu Fan, Andan Zhu, Melisa Kozaczek, Neethu Shah, Natalia Pabón-Mora, Favio González, Jeffrey P. Mower
Center for Plant Science Innovation: Faculty Publications
In parasitic plants, the reduction in plastid genome (plastome) size and content is driven predominantly by the loss of photosynthetic genes. The first completed mitochondrial genomes (mitogenomes) from parasitic mistletoes also exhibit significant degradation, but the generality of this observation for other parasitic plants is unclear. We sequenced the complete mitogenome and plastome of the hemiparasite Castilleja paramensis (Orobanchaceae) and compared them with additional holoparasitic, hemiparasitic and nonparasitic species from Orobanchaceae. Comparative mitogenomic analysis revealed minimal gene loss among the seven Orobanchaceae species, indicating the retention of typical mitochondrial function among Orobanchaceae species. Phylogenetic analysis demonstrated that the mobile cox1 …
Identification Of Homogentisate Dioxygenase As A Target For Vitamin E Biofortification In Oilseeds, Minviluz G. Stacey, Rebecca E. Cahoon, Hanh T. Nguyen, Yaya Cui, Shirley Sato, Cuong T. Nguyen, Nongnat Phoka, Kerry M. Clark, Yan Liang, Joe Forrester, Josef Batek, Phat Tien Do, David A. Sleper, Thomas E. Clemente, Edgar B. Cahoon, Gary Stacey
Identification Of Homogentisate Dioxygenase As A Target For Vitamin E Biofortification In Oilseeds, Minviluz G. Stacey, Rebecca E. Cahoon, Hanh T. Nguyen, Yaya Cui, Shirley Sato, Cuong T. Nguyen, Nongnat Phoka, Kerry M. Clark, Yan Liang, Joe Forrester, Josef Batek, Phat Tien Do, David A. Sleper, Thomas E. Clemente, Edgar B. Cahoon, Gary Stacey
Center for Plant Science Innovation: Faculty Publications
Soybean (Glycine max) is a major plant source of protein and oil and produces important secondary metabolites beneficial for human health. As a tool for gene function discovery and improvement of this important crop, a mutant population was generated using fast neutron irradiation. Visual screening of mutagenized seeds identified a mutant line, designated MO12, which produced brown seeds as opposed to the yellow seeds produced by the unmodified Williams 82 parental cultivar. Using forward genetic methods combined with comparative genome hybridization analysis, we were able to establish that deletion of the GmHGO1 gene is the genetic basis of …