Digital Commons Network^™

Characterization Of A Novel Mitochondrial Plasmid In Brassica, Mackenzie Strehle

UCARE: Research Products

Possessing some of the largest and most complex genomes of any eukaryotic organelles, plant mitochondria are notorious for their rapidly rearranging genetic framework. In addition to containing a large and complex mitochondrial genome, the mitochondria of several plants in the genus Brassica have also been shown to contain an independent, self-replicating linear plasmid. Interestingly, the plasmid appears to be able to move independently between the cytoplasm and the mitochondria, and it can be paternally inherited, unlike the rest of the mitochondrial genome. The plasmid also has features similar to those of adenoviruses, including terminal inverted repeats and covalently bound proteins …

Photosensing And Thermosensing By Phytochrome B Require Both Proximal And Distal Allosteric Features Within The Dimeric Photoreceptor, E Sethe Burgie, Adam N. Bussell, Shu-Hui Lye, Tong Wang, Weiming Hu, Katrice E. Mcloughlin, Erin L. Weber, Huilin Li, Richard D. Vierstra

Biology Faculty Research

Phytochromes (Phys) encompass a diverse collection of bilin-containing photoreceptors that help plants and microorganisms perceive light through photointerconversion between red light (Pr) and far-red light (Pfr)-absorbing states. In addition, Pfr reverts thermally back to Pr via a highly enthalpic process that enables temperature sensation in plants and possibly other organisms. Through domain analysis of the Arabidopsis PhyB isoform assembled recombinantly, coupled with measurements of solution size, photoconversion, and thermal reversion, we identified both proximal and distal features that influence all three metrics. Included are the downstream C-terminal histidine kinase-related domain known to promote dimerization and a conserved patch just upstream …

Survey Of Invasive Lantana Camara At Makirovana-Tsihomanaomby Forest Complex, Eliza Pessereau

Independent Study Project (ISP) Collection

Lantana camara is a shrub known globally as an invasive pest that grows primarily in degraded areas. The species is known to exist at Makirovana-Tsihomanaomby, a forest complex in northeastern Madagascar with 167 endemic species of flora and fauna, several of which are on the IUCN Red List. The complex, specifically Tsihomanaomby forest, is used as a resource for the three rural communes that live on its outskirts, meaning that it experiences much human activity. The objective of this study was to survey the population of L. camara at two sites: one just outside of the Tsihomanaomby forest and one …

Agricultural Responses To Climate Change: A Study Of Adaptive Farming Methods In Kizanda Village, Bailey Smith-Helman

Independent Study Project (ISP) Collection

Agriculture is vital to the economic and social systems in Tanzania, composing 30% of the country’s GDP as well as 80% of employment (FAO, 2014). Despite agriculture’s important role, it remains one of the most vulnerable sectors to climate change. Current trends project global average temperature to increase by 0.8-2.6 degrees Celsius, leaving farmers to face changes in rainfall, soil quality, and new pests and diseases (IPCC, 2007). Farmers will be forced to adapt to the changing climate if they are to sustain their livelihoods and the Tanzanian economy. For these reasons, it is important to understand the types of …

Shift In Icelandic Plant Populations Due To Climate Change: Through The Lens Of Natural Dyes, Molly Pluenneke

Independent Study Project (ISP) Collection

Northern residing plant species are at the highest risk for extinction due to temperature rise related to climate change (Schöb, Manuel, Choler & Veit, 2009). Climate change has also led to a northern shift in the geographic distribution of plant species (Parmesan & Yohe, 2003). This could lead to a necessary alteration in the way natural resources are utilized in arctic countries like Iceland (Lim-Camacho et al, 2017). The purpose of this study is to analyze the way in which Icelandic plant species used in natural dye practices may shift in distribution due to climate change and the potential impact …

Managing Opuntioid Cacti In Australia - Best Practice Control Manual For Austrocylindropuntia, Cylindropuntia And Optunia Species, M. R. Sheehan, S. Potter

Biosecurity published reports

The twenty-seven species of opuntioid cacti that are recognised as Weeds of National Significance are some of Australia's worst weeds. They grow over a wide climatic range and have impacted on pastoral and agricultural use of large tracts of land; pose an environmental risk to wildlife; and reduce aesthetic and recreational use, with direct impact on human safety.

Weedy infestations occur in all states and territories. Some are extensive, covering hundreds of thousands of hectares with a significant potential for range expansion

Over time there has been a substantial effort by government, industry, landholders and community groups to manage opuntioid …

Effects Of Nitrogen And Phosphorus Fertilizer And Topsoil Amendment On Native Plant Cover In Roadside Revegetation Projects, Heidi L. Hillhouse, Walter H. Schacht, Jonathan M. Soper, Carol E. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Establishing vegetation on roadsides following construction can be challenging, especially for relatively slow growing native species. Topsoil is generally removed during construction, and the surface soil following construction (“cut-slope soils”) is often compacted and low in nutrients, providing poor growing conditions for vegetation. Nebraska Department of Transportation (NDOT) protocols have historically called for nitrogen (N) and phosphorus (P) fertilization when planting roadside vegetation following construction, but these recommendations were developed for cool-season grass plantings and most current plantings use slower-establishing, native warmseason grasses that may benefit less than expected from current planting protocols. We evaluated the effects of nitrogen and …

Stability Of Cacopsylla Pyricola (Hemiptera: Psyllidae) Populations In Pacific Northwest Pear Orchards Managed With Long-Term Mating Disruption For Cydia Pomonella (Lepidoptera: Tortricidae), Kaushalya G. Amarasekare, Peter W. Shearer

Agricultural and Environmental Sciences Faculty Research

This study focused on conservation biological control of pear psylla, Cacopsylla pyricola, in the Pacific Northwest, USA. We hypothesized that insecticides applied against the primary insect pest, codling moth Cydia pomonella, negatively impact natural enemies of pear psylla, thus causing outbreaks of this secondary pest. Hence, the objective of this study was to understand how codling moth management influences the abundance of pear psylla and its natural enemy complex in pear orchards managed under long-term codling moth mating disruption programs. We conducted this study within a pear orchard that had previously been under seasonal mating disruption for codling moth for …

Phragmites Removal Increases Property Values In Michigan’S Lower Grand River Watershed, Paul Isely, Erik E. Nordman, Shaun Howard, Richard Bowman

Journal of Ocean and Coastal Economics

The presence of Phragmites australis, an invasive wetland plant, negatively affects coastal property values and home prices rise with distance from Phragmites. Home prices increased as distance to Phragmites increased at a rate of $3.90/meter. Removing Phragmites from a property so that the next closest Phragmites was 400 m away results in a property value increase of over $1,500. Removing all Phragmites within 400 m of any property results in a total property value impact of $837,000. This generates about $13,457-$15,121 in additional property taxes each year once the prices and taxes adjust to the plant’s removal. We …

Respons Tanaman Gandum Akibat Pemberian Sitokinin Berbagai Konsentrasi Dan Waktu Aplikasi Di Dataran Medium Jatinangor, Fiky Yulianto Wicaksono, Asyifa Fiani Putri, Yuyun Yuwariah, Yudithia Maxiselly, Tati Nurmala

Jurnal Kultivasi

Pertumbuhan dan hasil gandum (Triticum aestivum L.) diketahui dapat ditingkatkan dengan pemberian sitokinin. Sitokinin berperan juga dalam menunda penuaan daun gandum dan mengurangi cekaman panas di dataran medium. Penelitian ini bertujuan untuk mengetahui pengaruh interaksi berbagai konsentrasi sitokinin dengan berbagai waktu aplikasinya terhadap pertumbuhan dan hasil tanaman gandum. Penelitian menggunakan metode percobaan yang dilakukan di kebun percobaan Ciparanje Fakultas Pertanian Universitas Padjadjaran, Jatinangor, Sumedang mulai bulan November 2016 sampai dengan Februari 2017. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok, sementara rancangan perlakuan adalah faktorial. Perlakuan terdiri dari dua faktor, yaitu konsentrasi sitokinin (3 taraf) dan waktu aplikasi (5 taraf). …

Inheritance Study Of Resistance To The Herbicide Quizalofop-P-Ethyl In Rice And Development Of Provisia Hybrid Rice Germplasm, Jose Roberto Camacho Montero

LSU Doctoral Dissertations

In 2017, Provisia^TM quizalofop-p-ethyl (QFE) resistant rice became a new alternative for the chemical control of in-season red rice (Oryza sativa L. sp. spontaneum) infestations in Louisiana rice farms. Until then, Clearfield^® imidazolinone resistant rice was the only alternative that US farmers had for the efficient in-season chemical control of red rice. Provisia^TM rice is a BASF Corporation patented technology that can serve as an alternative for rotation with Clearfield^® rice. This strategy seeks to prevent outcrosses between red rice and herbicide resistant varieties, and consequently extend the life span of both technologies. This …

Phytochemical Screening And Determination Of Antibacterial, Anti-Tumorigenic And Dna Protection Ability Of Root Extracts Of Saussurea Lappa, Sughra Arif Minhas, Fida Muhammad Khan, Fakhar-I- Abbas, Abu Ul Hassan Faiz

Journal of Bioresource Management

Saussurea lappa is a traditionally well-known plant for its medicinal uses in different indigenous systems of medicine. It is widely used in the treatment of asthma, ulcer, stomach problems and inflammatory diseases. In current study the phytochemical screening of S. lappa showed the presence of sesquiterpenes, anthraquinones, lignans, alkaloids, flavonoids, glycosides and steroids. Antimicrobial potential of subject plant was also investigated against three bacterial strains and maximum antibacterial activity was observed by CEE against S. aureus, CAE against P. aeruginosa, while CME, CEE, EAE and PEE showed almost same significant activity against E. coli. The CME (1000 …

A First Linkage Map And Downy Mildew Resistance Qtl Discovery For Sweet Basil (Ocimum Basilicum) Facilitated By Double Digestion Restriction Site Associated Dna Sequencing (Ddradseq), Robert M. Pyne, Josh Honig, Jennifer Vaiciunas, Adolfina R. Koroch, Christian Wyenandt, Stacy Bonos, James Simon

Publications and Research

Limited understanding of sweet basil (Ocimum basilicum L.) genetics and genome structure has reduced efficiency of breeding strategies. This is evidenced by the rapid, worldwide dissemination of basil downy mildew (Peronospora belbahrii) in the absence of resistant cultivars. In an effort to improve available genetic resources, expressed sequence tag simple sequence repeat (EST-SSR) and single nucleotide polymorphism (SNP) markers were developed and used to genotype the MRI x SB22 F2 mapping population, which segregates for response to downy mildew. SNP markers were generated from genomic sequences derived from double digestion restriction site associated DNA sequencing (ddRADseq). Disomic segregation was observed …

The Gmo/Ge Debate, Joanna K. Sax

Texas A&M Law Review

We live longer and healthier lives because advances in science create easier and better ways to sustain and survive. Society has an intricate relationship with biotechnology. Vaccines save lives. Fluoridated water decreases dental issues. Antibiotics treat bacterial infections. Nuclear power is a form of clean energy. With any emerging technology, the benefits do not exist in a vacuum, thus, negative consequences result as well. Our widespread uses of antibiotics are creating antibiotic-resistant strains of bacteria. Our research into nuclear energy also facilitated the creation of nuclear bombs. Perhaps it is human nature to use scientific advances for good and for …

Recognizing Challenges And Opportunities In The Quest To End Hunger, Jennifer Williams Zwagerman

Texas A&M Law Review

As an attorney and professor that does not focus on intellectual property law, I was a bit apprehensive about providing a keynote address for a Symposium focusing on “Agriculture, Intellectual Property, and Feeding the World in the 21st Century.” As I thought about this topic, knowing that there were other speakers who would focus more on the IP issues and technical aspects of various topics, I kept coming back to the importance of technology as we worktowards the goal of feeding the world, and the many ways in which innovation plays a role in meeting that goal. It also brought …

Seeds From Fresh Conventional Tomatoes Germinate Faster Than Dried Or Organic Seeds, Christopher G. Brown, Kautz Evan, Cameron Kilpatrick, Mary Vu, Mallory Weaver

Georgia Journal of Science

Conventionally-grown and organically-grown tomato seeds sourced from both fresh tomatoes and store-bought packages were germinated for seven days to evaluate the effect of seed source on germination rates. Seeds from fresh Roma tomatoes were prepared by allowing them to ferment in their own pulp for 24 hours, while commercially packaged dry seeds did not require any preparation. Once prepared, the seeds were spaced evenly on wet paper towels and stored in resealable plastic bags in groups of 10 for a total sample size of 100 seeds in each of four treatments. The number of germinated seeds and the length of …

N2o Emissions From California Farmlands: A Review, Elizabeth Verhoeven, Engil Isadora Pujol Pereira, Charlotte Decock, Gina Garland, Taryn Kennedy, Emma Suddick, William Horwath, Johan Six

School of Earth, Environmental, & Marine Sciences Faculty Publications

Of the greenhouse gases emitted from cropland, nitrous oxide (N2O) has the highest global warming potential. The state of California acknowledges that agriculture both contributes to and is affected by climate change, and in 2016 it adopted legislation to help growers reduce emissions of greenhouse gases, explicitly including N2O. Nitrous oxide emissions can vary widely due to environmental and agronomic factors with most emission estimates coming from temperate grain systems. There is, however, a dearth of emission estimates from perennial and vegetable cropping systems commonly found in California's Mediterranean climate. Therefore, emission factors (EFs) specific to California conditions are needed …

First Interspecific Genetic Linkage Map For Castanea Sativa X Castanea Crenata Revealed Qtls For Resistance To Phytophthora Cinnamomi, Carmen Santos, Charles Dana Nelson, Tetyana Zhebentyayeva, Helena Machado, José Gomes-Laranjo, Rita Lourenço Costa

Forest Health Research and Education Center Faculty Publications

The Japanese chestnut (Castanea crenata) carries resistance to Phytophthora cinnamomi, the destructive and widespread oomycete causing ink disease. The European chestnut (Castanea sativa), carrying little to no disease resistance, is currently threatened by the presence of the oomycete pathogen in forests, orchards and nurseries. Determining the genetic basis of P. cinnamomi resistance, for further selection of molecular markers and candidate genes, is a prominent issue for implementation of marker assisted selection in the breeding programs for resistance. In this study, the first interspecific genetic linkage map of C. sativa x C. crenata …

Urban Impacts To Forest Productivity, Soil Quality, And Canopy Structure In Forest Park, Portland, Oregon, Andrew David Addessi

Dissertations and Theses

Land use practices and exposure to low impact disturbances associated with an urban environment can alter forest structure and function. Past and ongoing research in Forest Park, a large urban forest in Portland, Oregon, suggests that mature mixed Douglas-fir (Psuedotsuga meziesii)-hardwood stands in the more urban end of the park lack a shade-tolerant conifer understory composed of the late successional conifer tree species, such western hemlock (Tsuga heterophylla) and western red-cedar (Thuja plicata). 5-year plot remeasurement data that characterizes productivity and mortality patterns did not show a strong relationship to urban proximity. Plot productivity …

The Soybean Rfg1 Gene Restricts Nodulation By Sinorhizobium Fredii Usda193, Yinglun Fan, Jinge Liu, Shanhua Lyu, Qi Wang, Shengming Yang, Hongyan Zhu

Plant and Soil Sciences Faculty Publications

Sinorhizobium fredii is a fast-growing rhizobial species that can establish a nitrogen-fixing symbiosis with a wide range of legume species including soybeans (Glycine max). In soybeans, this interaction shows a high level of specificity such that particular S. fredii strains nodulate only a limited set of plant genotypes. Here we report the identification of a dominant gene in soybeans that restricts nodulation with S. fredii USDA193. Genetic mapping in an F2 population revealed co-segregation of the underlying locus with the previously cloned Rfg1 gene. The Rfg1 allele encodes a member of the Toll-interleukin receptor/nucleotide-binding site/leucine-rich repeat class of …

Antibacterial Activity Of Endophytic Actinomycetes Isolated From The Medicinal Plant Vochysia Divergens (Pantanal, Brazil), Francielly M. W. Gos, Daiani C. Savi, Khaled A. Shaaban, Jon S. Thorson, Rodrigo Aluizio, Yvelise M. Possiede, Jürgen Rohr, Chirlei Glienke

Pharmaceutical Sciences Faculty Publications

Endophytic actinomycetes from medicinal plants produce a wide diversity of secondary metabolites (SM). However, to date, the knowledge about endophytes from Brazil remains scarce. Thus, we analyzed the antimicrobial potential of 10 actinomycetes isolated from the medicinal plant Vochysia divergens located in the Pantanal sul-mato-grossense, an unexplored wetland in Brazil. Strains were classified as belonging to the Aeromicrobium, Actinomadura, Microbacterium, Microbispora, Micrococcus, Sphaerisporangium, Streptomyces, and Williamsia genera, through morphological and 16S rRNA phylogenetic analyzes. A susceptibility analysis demonstrated that the strains were largely resistant to the antibiotics oxacillin and nalidixic acid. Additionally, different culture media (SG and R5A), and …

Plant-Expressed Cocaine Hydrolase Variants Of Butyrylcholinesterase Exhibit Altered Allosteric Effects Of Cholinesterase Activity And Increased Inhibitor Sensitivity, Katherine E. Larrimore, I. Can Kazan, Latha Kannan, R. Player Kendle, Tameem Jamal, Matthew Barcus, Ashini Bolia, Stephen Brimijoin, Chang-Guo Zhan, S. Banu Ozkan, Tsafrir S. Mor

Molecular Modeling and Biopharmaceutical Center Faculty Publications

Butyrylcholinesterase (BChE) is an enzyme with broad substrate and ligand specificities and may function as a generalized bioscavenger by binding and/or hydrolyzing various xenobiotic agents and toxicants, many of which target the central and peripheral nervous systems. Variants of BChE were rationally designed to increase the enzyme’s ability to hydrolyze the psychoactive enantiomer of cocaine. These variants were cloned, and then expressed using the magnICON transient expression system in plants and their enzymatic properties were investigated. In particular, we explored the effects that these site-directed mutations have over the enzyme kinetics with various substrates of BChE. We further compared the …

Plant Mechanosensitive Ion Channels: An Ocean Of Possibilities, Debarati Basu, Elizabeth S. Haswell

Biology Faculty Research

Mechanosensitive ion channels, transmembrane proteins that directly couple mechanical stimuli to ion flux, serve to sense and respond to changes in membrane tension in all branches of life. In plants, mechanosensitive channels have been implicated in the perception of important mechanical stimuli such as osmotic pressure, touch, gravity, and pathogenic invasion. Indeed, three established families of plant mechanosensitive ion channels play roles in cell and organelle osmoregulation and root mechanosensing - and it is likely that many other channels and functions await discovery. Inspired by recent discoveries in bacterial and animal systems, we are beginning to establish the conserved and …

Nebline, September 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Early Childhood Educators Benefit from Mindfulness Reflection

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

Managing Nitrous Oxide Emissions In Agricultural Fields, Mark S. Coyne, Wei Ren

Plant and Soil Sciences Research Report

Agriculture is a major contributor to atmospheric nitrous oxide (N₂O) (Smith et al., 2014; Tian et al., 2015). Unfortunately, nitrous oxide destroys stratospheric ozone (O₃) which protects us from ultraviolet radiation (Cicerone, 1989) and it increases ground level O_3, whichis an air pollutant threatening human health and food production. Nitrous oxide is also 298 times more potent than an equivalent amount of carbon dioxide (CO₂) in terms of trapping and absorbing reflected solar radiation (Forster et al., 2007). Basic chemistry and physics assure us that increased levels of N₂O in the …

Summaries Of Arkansas Cotton Research 2016, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

A Comparative Genome Analysis Of Cercospora Sojina With Other Members Of The Pathogen Genus Mycosphaerella On Different Plant Hosts, Fanchang Zeng, Xin Lian, Guirong Zhang, Xiaoman Yu, Carl A. Bradley, Ray Ming

Plant Pathology Faculty Publications

Fungi are the causal agents of many of the world's most serious plant diseases causing disastrous consequences for large-scale agricultural production. Pathogenicity genomic basis is complex in fungi as multicellular eukaryotic pathogens. Here, we report the genome sequence of C. sojina, and comparative genome analysis with plant pathogen members of the genus Mycosphaerella (Zymoseptoria. tritici (synonyms M. graminicola), M. pini, M. populorum and M. fijiensis - pathogens of wheat, pine, poplar and banana, respectively). Synteny or collinearity was limited between genomes of major Mycosphaerella pathogens. Comparative analysis with these related pathogen genomes indicated distinct genome-wide repeat …

Tropical Cyclones Disrupt The Relationship Between Tree Height And Species Diversity: Comment, Teng-Chiu Lin, Lixin Wang, Chengyang Zheng, Ryan W. Mcewan, Chung-Te Chang, Jyh-Min Chiang, Chung-Hao Chi

Biology Faculty Publications

In a recent report on the patterns of tree species richness in eastern and western North America, Marks et al. (2016) claimed to have identified an operational indicator of environmental harshness (maximum tree height) and concluded that environmental stressors that limit tree height also act as ecological filters on species richness. Marks et al. (2017) attributed the positive association between species richness and maximum tree height to both the direct effects of environmental harshness on species richness and the indirect effects of environmental harshness on species richness as mediated by maximum tree height.

This finding overlooked the fact that many …

Reciprocal Effect Of Parental Lines On The Physiological Potential And Seed Composition Of Corn Hybrid Seeds, Juliana F. Santos, Lynnette M. A. Dirk, A. Bruce Downie, Mauricio F. G. Sanches, Roberval D. Vieira

Horticulture Faculty Publications

Obtaining corn hybrid seeds (Zea mays L.) with high vigour depends on the parental lines and the direction of the cross, and this relates to seed desiccation tolerance and composition. This research studied reciprocal crosses between pairs of proprietary, elite parent lines (L1 and L5; L2 and L4) producing hybrid seeds with different qualities attempting to correlate vigour with seed composition, focusing on storage proteins, starch and soluble sugar amounts. Four corn hybrid seed lots produced from reciprocal crosses were compared (HS 15 with HS 51, and HS 24 with HS 42) by assessing germination, vigour, and seedling emergence …

Warming And Elevated Co2 Alter The Suberin Chemistry In Roots Of Photosynthetically Divergent Grass Species, Vidya Suseela, Nishanth Tharayil, Elise Pendall, Apparao M. Rao

Publications

A majority of soil carbon (C) is either directly or indirectly derived from fine roots, yet roots remain the least understood component of the terrestrial carbon cycle. The decomposability of fine roots and their potential to contribute to soil C is partly regulated by their tissue chemical composition. Roots rely heavily on heteropolymers such as suberins, lignins and tannins to adapt to various environmental pressures and to maximize their resource uptake functions. Since the chemical construction of roots is partly shaped by their immediate biotic/abiotic soil environments, global changes that perturb soil resource availability and plant growth could potentially alter …