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Effects Of Red Clover Isoflavones On Tall Fescue Seed Fermentation And Microbial Populations In Vitro, Emily A. Melchior, Jason K. Smith, Liesel G. Schneider, Travis Mulliniks, Gary E. Bates, Zachary D. Mcfarlane, Michael D. Flythe, James L. Klotz, Jack P. Goodman, Huihua Ji, Phillip R. Meyer Jan 2018

Effects Of Red Clover Isoflavones On Tall Fescue Seed Fermentation And Microbial Populations In Vitro, Emily A. Melchior, Jason K. Smith, Liesel G. Schneider, Travis Mulliniks, Gary E. Bates, Zachary D. Mcfarlane, Michael D. Flythe, James L. Klotz, Jack P. Goodman, Huihua Ji, Phillip R. Meyer

West Central Research and Extension Center, North Platte

Negative impacts of endophyte-infected Lolium arundinaceum (Darbyshire) (tall fescue) are responsible for over $2 billion in losses to livestock producers annually. While the influence of endophyte-infected tall fescue has been studied for decades, mitigation methods have not been clearly elucidated. Isoflavones found in Trifolium pratense (red clover) have been the subject of recent research regarding tall fescue toxicosis mitigation. Therefore, the aim of this study was to determine the effect of ergovaline and red clover isoflavones on rumen microbial populations, fiber degradation, and volatile fatty acids (VFA) in an in vitro system. Using a dose of 1.10 mg …


Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 19 2018, Several Authors Jan 2018

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 19 2018, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Agricultural Information Needs And Food Access In The Stann Creek District Of Belize, Sam E. Harris, Donna L. Graham Jan 2018

Agricultural Information Needs And Food Access In The Stann Creek District Of Belize, Sam E. Harris, Donna L. Graham

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The purpose of this study was to describe agricultural information sources available to farmers and to describe food access and availability for the people of Dangriga, Stann Creek, Belize. This study used descriptive survey research methods with convenience sampling of the general public (n=22) and of farmers (n = 38) in the summer of 2017. Farmers use a variety of agricultural information sources with the extension service cited most often, followed by friends and fellow farmers. Weather, lack of information, pests, and inadequate access to capital were of primary concern for farmers. Face-to-face meetings were used most often by extension …


Tripartite Interactions Of Legumes With Arbuscular Mycorrhizal Fungi And Rhizobial Bacteria: Insight Into Plant Growth, Seed Yield, And Resource Exchange, Arjun Kafle Jan 2018

Tripartite Interactions Of Legumes With Arbuscular Mycorrhizal Fungi And Rhizobial Bacteria: Insight Into Plant Growth, Seed Yield, And Resource Exchange, Arjun Kafle

Electronic Theses and Dissertations

Under natural conditions, legumes, such as alfalfa (Medicago) and soybean (Glycine max) are colonized with arbuscular mycorrhizal (AM) fungi and rhizobial bacteria forming tripartite interactions. Legumes are important crop species due to their high nutritional and economic values. Most of the previous literatures focused on experiments with an individual symbiont: either AM fungi or rhizobial bacteria, but not with both symbionts at the same time, thus our current understanding of resource exchange in tripartite interactions is limited. It has been reported that AM fungi primarily provide phosphate (P), nitrogen (N), and other nutritional and non-nutritional benefits while rhizobial bacteria solely …


Genetic Component Effects Of Two Loci On Seed Dormancy, Awn, Low-Temperature Germination, Plant Height, And Flowering Time In Rice (Oryza Sativa L.), Ugur Korkmaz Jan 2018

Genetic Component Effects Of Two Loci On Seed Dormancy, Awn, Low-Temperature Germination, Plant Height, And Flowering Time In Rice (Oryza Sativa L.), Ugur Korkmaz

Electronic Theses and Dissertations

Seed dormancy (SD) is an adaptive trait of both ecological and agricultural importance. Cereal crops have been selected for reduced SD to promote germination, which also caused the pre-harvest sprouting (PHS) problem in crop production. The previous research identified a set of quantitative trait loci (QTL) for SD, including qSD1- 2 and qSD8, in the conspecific weedy and cultivated rice (Oryza sativa). qSD1-2 carries a dormancy-enhancing allele from the cultivated rice and has a pleiotropic effect on plant height. qSD8 carries a dormancy-enhancing allele from the weedy rice and tightly links to qAL8 for awn length. The …


Exploiting The Genetic Diversity Of Wild Ancestors And Relatives Of Wheat For Its Improvement, Jagdeep Singh Sidhu Jan 2018

Exploiting The Genetic Diversity Of Wild Ancestors And Relatives Of Wheat For Its Improvement, Jagdeep Singh Sidhu

Electronic Theses and Dissertations

Wheat is the third most staple food worldwide but current 1% annual improvement in the wheat production is insufficient to meet the growing demands in future. The narrow genetic base of wheat limits continuous improvement in wheat productivity and tolerance to biotic and abiotic stresses under changing climate. Wild ancestors and relatives of wheat hold a potential in widening the genetic pool of wheat and enhance its resilience to biotic and abiotic stresses. This study was focused towards characterizing the genetic diversity in wild relatives of wheat for disease resistance and efficient association with diazotrophs. In the first study, we …


Application Of Genomic Approaches To Improve Yield And Bacterial Leaf Streak Resistance In Winter Wheat, Sai Mukund Ramakrishnan Jan 2018

Application Of Genomic Approaches To Improve Yield And Bacterial Leaf Streak Resistance In Winter Wheat, Sai Mukund Ramakrishnan

Electronic Theses and Dissertations

Global wheat production is threatened by the change in climate thus leading lead to the increase in the biotic and abiotic stresses. We need to increase wheat productivity at a faster pace and manage these challenges to meet the growing demand. Development of cultivars with durable disease resistance and enhancing the rate of genetic gain in wheat are the major goals in wheat breeding programs. Bacterial Leaf Streak (BLS) is one of the most threatening bacterial diseases to wheat in the US Northern Great Plains. Unlike fungal diseases, bacterial diseases cannot be effectively managed using chemicals and thus developing disease …


Study Of Seasonal Change And Water Stress Condition In Plant Leaf Using Polarimetric Lidar Measurement, Prabeen Kattel Jan 2018

Study Of Seasonal Change And Water Stress Condition In Plant Leaf Using Polarimetric Lidar Measurement, Prabeen Kattel

Electronic Theses and Dissertations

Study of vegetation is of great importance to the improvement of agriculture and forest management. Although there have been various attempts to characterize vegetation using remote sensing techniques, polarimetric lidar is a novel remote sensing tool that has shown potential in vegetation remote sensing. In this thesis, a near-infrared polarimetric lidar at 1064 nm was used to investigate the effects of seasonal change and water stress condition on plant leaves. Two variables, time and water content, affected the plant leaf laser depolarization ratio measurement. The first study focused on the maple tree in order to figure out how seasonal change …


Air-Propelled Organic Fertilizer Grits Can Be Used To Control Weeds And Provide Nitrogen, Michael Carlson Jan 2018

Air-Propelled Organic Fertilizer Grits Can Be Used To Control Weeds And Provide Nitrogen, Michael Carlson

Electronic Theses and Dissertations

Weeds are one of the biggest challenges for organic growers because of the alternative weed control methods. Air-propelled abrasive grit management has been reported to control weed seedlings in corn and soybeans while maintaining yield. This research examined the weed control, corn and soybean yields, nitrogen mineralization, and nitrogen yield responses from grits. The grits used in this research included: Phytaboost Plant Food 7-1-2 (soybean meal), Sustane 8-2-4 and 4-6-4 (turkey litters), and two non-fertilizer grits: Agra Grit (walnut shells) and corn cob meal. Field studies were conducted from 2015 to 2017 in Aurora, SD, Beresford, SD and Morris, MN. …


Insecticidal Seed And In-Furrow Treatment Recommendations For Soybean And Sunflower, Brady Hauswedell Jan 2018

Insecticidal Seed And In-Furrow Treatment Recommendations For Soybean And Sunflower, Brady Hauswedell

Electronic Theses and Dissertations

Throughout the 2016 and 2017 growing season, field research experiments were replicated across South Dakota. Many times seed treatments are used prophylactic, which is neither good for the producers or the environment. Producers will be able to reduce production costs, if they only use a seed treatment when necessary. The purpose of the first experiment was to determine the effects of seed treatments in combination with planting date and seeding rate on soybean yield. To determine the effects, two years of field data from four eastern South Dakota locations were compared. Within each year and location we compared two planting …


Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler Jan 2018

Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler

Electronic Theses and Dissertations

Plant feedstuff by-products such as soy processing wastewater, guar korma meal, and sorghum hominy are very different, however, all possess a rich nutrient profile. Lessening their value is the presence of lignocellulose, plant anti-nutritional factors, and unfavorable protein profiles. Fungal conversion processes are an attractive approach to improving the value of these by-products by degrading detrimental fractions of each while simultaneously creating nutrient-rich cell mass. The aim of this research was to improve the value of each by-product for potential application in monogastric diets such as fish. Fungal organisms, both yeast and filamentous fungi, were examined for their ability to …


Winter Surveys Of Cotinus Obovatus (American Smoketree) In The Ozark Mountains, Gary R. Graves Jan 2018

Winter Surveys Of Cotinus Obovatus (American Smoketree) In The Ozark Mountains, Gary R. Graves

Journal of the Arkansas Academy of Science

Cotinus obovatus (American smoketree) is a rare deciduous tree with a relictual distribution in southeastern North America. Efforts to map its fine-scale geographic distribution in the Ozark Mountains have been limited to the growing season when the distinctive blooming panicles and foliage facilitate detection in hardwood-cedar woodlands. I describe the physiognomic traits of leafless C. obovatus that permit effective population mapping in winter landscapes. Clumped growth and diagonally leaning stems facilitate detection at a distance. Bark texture, twig morphology, and sap odor confirm the identity of the tree at close range.


Black Cohosh Seed Germination And Conservation, W. John Hayden Jan 2018

Black Cohosh Seed Germination And Conservation, W. John Hayden

Biology Faculty Publications

Like many plant enthusiasts, I have spent a considerable amount of time planting seeds. Every year I grow many vegetables—my garden always includes some annual bedding plants—and I sow seeds of cover crops (winter wheat, winter rye, and buckwheat) by the tens of thousands. While I have committed vast numbers of propagules to moist soil, I cannot say that I have watched every single one sprout. Nevertheless, I certainly have observed the germination process many, many times for lots of different seeds. For these seeds of garden plants, germination is quite rapid, just a few days to maybe as much …


Characterization Of Neofabraea Actinidiae And N. Brasiliensis As Causal Agents Of Apple Bull’S-Eye Rot In Southern Brazil, Amauri Bogo, Carla C. Comparin, Rosa M. Valdebenito Sanhueza, Patricia Ritschel, Ricardo T. Casa, Fábio N. Silva, Sydney E. Everhart Jan 2018

Characterization Of Neofabraea Actinidiae And N. Brasiliensis As Causal Agents Of Apple Bull’S-Eye Rot In Southern Brazil, Amauri Bogo, Carla C. Comparin, Rosa M. Valdebenito Sanhueza, Patricia Ritschel, Ricardo T. Casa, Fábio N. Silva, Sydney E. Everhart

Department of Plant Pathology: Faculty Publications

The causal agents of apple bull’s-eye rot in southern Brazil have recently been described as Neofabraea actinidiae and N. brasiliensis. Isolates of both species were evaluated for response of mycelial growth index (MGI) to different temperatures, enzyme production, mycelial growth inhibition and effective concentrations (EC50 and EC100) of the fungicides triflumizole, pyrimethanil and thiophanate methyl, as well as aggressiveness on fruits of ‘Fuji’ hybrid and ‘Pink Lady’. There was significantly lower mycelium growth in N. brasiliensis compared with N. actinidiae at all temperatures tested. Neither species grew at 3 and 32°C. There were minor differences in …


Elucidating The Role Of Highly Homologous Nicotiana Benthamiana Ubiquitin E2 Gene Family Members In Plant Immunity Through An Improved Virus‑Induced Gene Silencing Approach, Bangjun Zhou, Lirong Zeng Jan 2018

Elucidating The Role Of Highly Homologous Nicotiana Benthamiana Ubiquitin E2 Gene Family Members In Plant Immunity Through An Improved Virus‑Induced Gene Silencing Approach, Bangjun Zhou, Lirong Zeng

Department of Plant Pathology: Faculty Publications

Background: Virus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a high-throughput manner. However, the specificity and efficiency for knocking down members of a highly homologous gene family have remained to date a significant challenge in VIGS due to silencing of off-targets. Results: Here we present an improved method for the selection and evaluation of gene fragments used for VIGS to specifically and efficiently knock down members of a highly homologous gene family. Using this method, we knocked down …


The Tomato U-Box Type E3 Ligase Pub13 Acts With Group Iii Ubiquitin E2 Enzymes To Modulate Fls2-Mediated Immune Signaling, Bangjun Zhou, Lirong Zeng Jan 2018

The Tomato U-Box Type E3 Ligase Pub13 Acts With Group Iii Ubiquitin E2 Enzymes To Modulate Fls2-Mediated Immune Signaling, Bangjun Zhou, Lirong Zeng

Department of Plant Pathology: Faculty Publications

In Arabidopsis and rice, the ubiquitin ligase PUB13-mediated protein degradation plays a significant role in plant pattern-triggered immunity (PTI) and flowering time control. The Arabidopsis PUB13 has been shown to attenuate the pattern recognition receptor FLS2-mediated immune signaling by ubiquitinating FLS2 and consequently promoting its degradation by the 26S proteasome. Nevertheless, the cognate ubiquitin-conjugating enzymes (E2) with which PUB13 acts to modulate FLS2-mediated PTI are unknown. To address this question, we investigate here the tomato (Solanum lycopersicum) homolog of PUB13, SlPUB13 by utilizing the recently characterized complete set of tomato E2s. Of the 13 groups of tomato E2s, …


Proteomic Analysis Of Ubiquitinated Proteins In Rice (Oryza Sativa) After Treatment With Pathogen-Associated Molecular Pattern (Pamp) Elicitors, Xiao-Lin Chen, Xin Xie, Liye Wu, Caiyun Liu, Lirong Zeng, Xueping Zhou, Feng Luo, Guo-Liang Wang, Wende Liu Jan 2018

Proteomic Analysis Of Ubiquitinated Proteins In Rice (Oryza Sativa) After Treatment With Pathogen-Associated Molecular Pattern (Pamp) Elicitors, Xiao-Lin Chen, Xin Xie, Liye Wu, Caiyun Liu, Lirong Zeng, Xueping Zhou, Feng Luo, Guo-Liang Wang, Wende Liu

Department of Plant Pathology: Faculty Publications

Reversible protein ubiquitination plays essential roles in regulating cellular processes. Although many reports have described the functions of ubiquitination in plant defense responses, few have focused on global changes in the ubiquitome. To better understand the regulatory roles of ubiquitination in rice pattern-triggered immunity (PTI), we investigated the ubiquitome of rice seedlings after treatment with two pathogen-associated molecular patterns, the fungal-derived chitin or the bacterialderived flg22, using label-free quantitative proteomics. In chitin-treated samples, 144 and 167 lysine-ubiquitination sites in 121 and 162 proteins showed increased and decreased ubiquitination, respectively. In flg22-treated samples, 151 and 179 lysine-ubiquitination sites in 118 and …


Genes For Membrane Transport Proteins: Not So Rare In Viruses, Timo Greiner, Anna Moroni, James L. Van Etten, Gerhard Thiel Jan 2018

Genes For Membrane Transport Proteins: Not So Rare In Viruses, Timo Greiner, Anna Moroni, James L. Van Etten, Gerhard Thiel

James Van Etten Publications

Some viruses have genes encoding proteins with membrane transport functions. It is unknown if these types of proteins are rare or are common in viruses. In particular, the evolutionary origin of some of the viral genes is obscure, where other viral proteins have homologs in prokaryotic and eukaryotic organisms. We searched virus genomes in databases looking for transmembrane proteins with possible transport function. This effort led to the detection of 18 different types of putative membrane transport proteins indicating that they are not a rarity in viral genomes. The most abundant proteins are K+ channels. Their predicted structures vary between …


Size-Dependent Catalysis Of Chlorovirus Population Growth By A Messy Feeding Predator, John Delong, Zeina Al-Ameeli, Shelby Lyon, James L. Van Etten, David Dunigan Jan 2018

Size-Dependent Catalysis Of Chlorovirus Population Growth By A Messy Feeding Predator, John Delong, Zeina Al-Ameeli, Shelby Lyon, James L. Van Etten, David Dunigan

James Van Etten Publications

Many chloroviruses replicate in endosymbiotic zoochlorellae that are protected from infection by their symbiotic host. To reach the high virus concentrations that often occur in natural systems, a mechanism is needed to release zoochlorellae from their hosts. We demonstrate that the ciliate predator Didinium nasutum foraging on zoochlorellae-bearing Paramecium bursaria can release live zoochlorellae from the ruptured prey cell that can then be infected by chloroviruses. The catalysis process is very effective, yielding roughly 95% of the theoretical infectious virus yield as determined by sonication of P. bursaria. Chlorovirus activation is more effective with smaller Didinia, as larger …


Influence Of Kernel Shape And Size On The Packing Ratio And Compressibility Of Hard Red Winter Wheat, Marvin C. Petingco, Mark E. Casada, Ronaldo G. Maghirang, Sidney A. Thompson, Samuel G. Mcneill, Michael D. Montross, Aaron P. Turner Jan 2018

Influence Of Kernel Shape And Size On The Packing Ratio And Compressibility Of Hard Red Winter Wheat, Marvin C. Petingco, Mark E. Casada, Ronaldo G. Maghirang, Sidney A. Thompson, Samuel G. Mcneill, Michael D. Montross, Aaron P. Turner

Research and Education Center at Princeton Faculty Publications

Grain compaction occurs during bin storage, and its determination is important for the grain mass estimation needed for inventory and auditing. The degree of compaction is dependent on grain type, bin type, moisture content, amount of grain, initial grain bulk density, coefficients of friction, lateral-to-vertical pressure coefficient, and variation in kernel size. Previous studies have correlated several of these parameters, such as bulk density and grain packing, with moisture content. This study investigated the influence of wheat kernel shape and size distribution on packing ratio and compressibility. Two dockage-free hard red winter (HRW) wheat samples, with no shrunken or broken …


Investigating Phosphorylation Patterns And Their Effect On The Activity Of Transcription Factor Taabf1 In Imbibing Cereal Grains, Grace Uwase Jan 2018

Investigating Phosphorylation Patterns And Their Effect On The Activity Of Transcription Factor Taabf1 In Imbibing Cereal Grains, Grace Uwase

Honors Theses

The wheat transcription factor TaABF1 plays an important role in hormone-mediated regulation of seed dormancy and germination of cereal grains. Evidence shows that TaABF1 activity is regulated by phosphorylation, and previous work in our lab showed that when serine residues in its conserved regions; S36, S37, S113, S115 were altered to phosphomimetic aspartate, the 4xD TaABF1 mutant had increased activity as a transcription factor. However, when only S113 was altered, TaABF1’s activity was greatly reduced. The work presented here explored whether the S36D/S37D/S115D mutant would have stronger activity than the 4xD mutant. Using the particle bombardment technique to introduce the …


Medicinal Properties Of Cyperus Species (Sedge Family, Cyperaceae), Lata Maishaya Udari Jan 2018

Medicinal Properties Of Cyperus Species (Sedge Family, Cyperaceae), Lata Maishaya Udari

Masters Theses

The grasses that yield therapeutically important products are among the least studied in the Cyperaceae family. Herb-based medicine from ancient times have played a vital role in ailments of various disease and nowadays it has been a particular area of interest in medicine. Phytochemicals are inherent compounds derived from plants that are biologically active, non-nutritive chemicals that act as a defensive or prophylactic medicine in humans. Their extracts have proven to show inhibition properties against different microbes. Despite the prevalence of Cyperus species and their traditional use in medicine, the chemical components responsible for their attributes remain largely unknown. This …


Co-Product Potential Of Algae Biocake, Elizabeth J. Nixon Jan 2018

Co-Product Potential Of Algae Biocake, Elizabeth J. Nixon

Masters Theses

Society needs to find replacements for fossil fuels, which are finite resources that may be fully depleted within a few generations. While solar and wind have great potential as alternative energy sources, they are unlikely to completely replace all of the current fuel sources, particularly liquid fuels. Plant biomass has great potential for this market, and is already used in many forms for heat energy (e.g. direct combustion). However, the bulk density of important bioenergy crops, particularly grasses, is often low, which necessitates the use of binders in densification strategies. In the present study, waste algae biocake from a proprietary …


Distiphallus Morphology And Its Role In Copulation Dynamics In Anastrepha Suspensa (Loew), Taylor J. Inboden Jan 2018

Distiphallus Morphology And Its Role In Copulation Dynamics In Anastrepha Suspensa (Loew), Taylor J. Inboden

Masters Theses

Anastrepha suspensa (Diptera; Tephritidae) is an agricultural pest species causing severe economic damage and is controlled, in part, by applying knowledge of this species' reproduction (e.g., disrupting fertile copulations by SIT). During copulation, males transfer sperm as well as protein rich fluids through an aedeagus and distiphallus, which females then store in three spermathecae and one ventral receptacle. Within the female reproductive tract, the ventral receptacle and the three spermathecae are strategically separated from each other. I hypothesized males' ability to direct sperm transfer within the female reproductive tract is through the structures found in the distiphallus. Utilizing scanning electron …


New Species, New Combinations And New Synonymies Towards A Treatment Of Acanthaceae For The Manual De Plantas De Costa Rica, Lucinda A. Mcdade, Barry E. Hammel, Carrie A. Kiel Jan 2018

New Species, New Combinations And New Synonymies Towards A Treatment Of Acanthaceae For The Manual De Plantas De Costa Rica, Lucinda A. Mcdade, Barry E. Hammel, Carrie A. Kiel

Aliso: A Journal of Systematic and Floristic Botany

In preparation for the publication of the Manual de Plantas de Costa Rica, new species, names, combinations, and synonymies are provided in six genera of Acanthaceae: Anisacanthus, Chamaeranthemum, Dicliptera, Justicia, Ruellia and Stenostephanus. The new species are A. grace-woodiae, J. altior, J. lithophila and S. chavesii. A new name at the species level, R. leonardiana, is provided for R. tubiflora var. hirsuta. With Habracanthus, Hansteinia, Kalbreyeriella and Razisea being subsumed within Stenostephanus, the new combinations S. blepharorhachis, S. citrinus, S. leiorhachis (= Razisea spicata …


Measuring The Influence Of Bedding On Soil Nitrogen Losses And Corn Crop Nitrogen Characteristics For Fall Applied Solid Beef Cattle Manure In Eastern South Dakota, Mukesh Mehata Jan 2018

Measuring The Influence Of Bedding On Soil Nitrogen Losses And Corn Crop Nitrogen Characteristics For Fall Applied Solid Beef Cattle Manure In Eastern South Dakota, Mukesh Mehata

Electronic Theses and Dissertations

Nitrogen (N) is a major component of chlorophyll which plays a key role in the photosynthesis process in crops. The N is one of the highest demanded nutrients by all plants for their growth and reproduction. Manure or inorganic fertilizer is often applied to fulfill the crops’ N demand. However, the applied N sources have the potential of N losses in different forms from the soil volume in many ways such as ammonia (NH3) volatilization, aerial nitrous oxide (N2O) loss, nitrate (NO3 --N) leaching, and runoff and/or erosion. Soil fertility, crop yield, water quality, and …


Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa Jan 2018

Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa

Electronic Theses and Dissertations

Root provides plant water, nutrients and anchorage from soil. Most our knowledge of molecular mechanisms of root development is from the dicot model plant Arabidopsis, but very few studies have done in monocot crop systems like rice, maize, and wheat. We are studying very short root (VSR) phenotype in wheat, and lack of a sequenced reference genome in wheat prompted us to sequence and assemble the root transcriptome of the reference cultivar Chinese Spring (CS). A root transcriptome was assembled from the sequenced reads generated from root tip and the mature root tissues of CS. Approximately 169 million reads were …


Efficacy Of Seed Treatments, Microbial And Biochemical Pesticides For Managing Early Tan Spot And Stripe Rust Diseases Of Wheat, Collins Bugingo Jan 2018

Efficacy Of Seed Treatments, Microbial And Biochemical Pesticides For Managing Early Tan Spot And Stripe Rust Diseases Of Wheat, Collins Bugingo

Electronic Theses and Dissertations

Wheat is a major cereal crop in the U.S.A and in the world. However, its production is hampered by a number of factors both biotic and abiotic. Foliar diseases like tan spot caused by Pyrenophora tritici-repentis and stripe rust caused by Puccinia striiformis f. sp. tritici, are economically important diseases causing over 5-55 % yield loss. To assess the efficacy of fungicide seed treatments in the management of early tan spot, two hard red spring wheat cultivars “Select” and “Brick” were treated with seedapplied commercially available combo fungicides metalaxyl + pyraclostrobin + triticonazole and difenoconazole + mefenoxam + sedaxane …


Impacts Of Diverse Crop Rotations And Integrated Crop-Livestock System On Soil Quality In South Dakota, Atilla Polat Jan 2018

Impacts Of Diverse Crop Rotations And Integrated Crop-Livestock System On Soil Quality In South Dakota, Atilla Polat

Electronic Theses and Dissertations

Crop diversification can be beneficial in improving soil quality parameters. Diverse crop rotations and integrated crop-livestock systems (ICLS), are two examples of crop diversification used in this study to assess soil quality. The objectives of this study were to: (i) assess the impacts of diverse rotations on soil quality parameters, and (ii) assess the impacts of low and high stocking density on soil quality parameters. Both of the studies were conducted separately and are presented as two different chapters. To accomplish objective (i), a long-term experiment was selected which was established near Brookings, South Dakota, USA at USDA-ARS lab in …


Soybean Iron Deficiency Chlorosis: Quantitative Trait Locus Validation And Grain Yield Evaluation, Ahmed Charif Jan 2018

Soybean Iron Deficiency Chlorosis: Quantitative Trait Locus Validation And Grain Yield Evaluation, Ahmed Charif

Electronic Theses and Dissertations

Soybean production in most of the Northern Great Plain area has been challenged by iron deficiency chlorosis (IDC), which is a physiological problem with a plant grown in high pH, calcareous soil. Developing IDC-resistant cultivars is the best approach to meet this challenge. Currently, this approach is limited by lack of knowledge about genetic resources and mechanisms for resistance to IDC. The objectives of this research were to validate quantitative trait loci (QTL) associated with IDC and to evaluate the effect of IDC on yield in soybean cultivars. To validate the QTL, a population of 201 recombinant inbred lines, which …