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Articles 15241 - 15270 of 26354
Full-Text Articles in Entire DC Network
Phosphorus Limitation Of Aboveground Production In Northern Hardwood Forests, Shinjini Goswami, Melany C. Fisk, Matthew A. Vadeboncoeur, Mariann Garrison-Johnston, Ruth D. Yanai, Timothy J. Fahey
Phosphorus Limitation Of Aboveground Production In Northern Hardwood Forests, Shinjini Goswami, Melany C. Fisk, Matthew A. Vadeboncoeur, Mariann Garrison-Johnston, Ruth D. Yanai, Timothy J. Fahey
All Materials
Forest productivity on glacially derived soils with weatherable phosphorus (P) is expected to be limited by nitrogen (N), according to theories of long-term ecosystem development. However, recent studies and model simulations based on resource optimization theory indicate that productivity can be co-limited by N and P. We conducted a full factorial N × P fertilization experiment in 13 northern hardwood forest stands of three age classes in central New Hampshire, USA, to test the hypothesis that forest productivity is co-limited by N and P. We also asked whether the response of productivity to N and P addition differs among species …
Hydrological Niche Segregation Defines Forest Structure And Drought Tolerance Strategies In A Seasonal Amazon Forest, Mauro Brum, Matthew A. Vadeboncoeur, Valeriy Ivanov, Heidi Asbjornsen, Scott Saleska, Luciana F. Alves, Deliane Pinha, Jadson D. Dias, Luiz E O C Aragão, Fernanda Barros, Paulo Bittencourt, Luciano Pereira, Rafael S. Oliveira
Hydrological Niche Segregation Defines Forest Structure And Drought Tolerance Strategies In A Seasonal Amazon Forest, Mauro Brum, Matthew A. Vadeboncoeur, Valeriy Ivanov, Heidi Asbjornsen, Scott Saleska, Luciana F. Alves, Deliane Pinha, Jadson D. Dias, Luiz E O C Aragão, Fernanda Barros, Paulo Bittencourt, Luciano Pereira, Rafael S. Oliveira
All Materials
- The relationship between rooting depth and above‐ground hydraulic traits can potentially define drought resistance strategies that are important in determining species distribution and coexistence in seasonal tropical forests, and understanding this is important for predicting the effects of future climate change in these ecosystems.
- We assessed the rooting depth of 12 dominant tree species (representing c. 42% of the forest basal area) in a seasonal Amazon forest using the stable isotope ratios (δ18O and δ2H) of water collected from tree xylem and soils from a range of depths. We took advantage of a major ENSO‐related …
Floral Symmetry Genes Elucidate The Development And Evolution Of Oil-Bee Pollinated Flowers Of Malpighiaceae And Krameriaceae, Farahnoz N. Khojayori
Floral Symmetry Genes Elucidate The Development And Evolution Of Oil-Bee Pollinated Flowers Of Malpighiaceae And Krameriaceae, Farahnoz N. Khojayori
Theses and Dissertations
Specialization on insect and animal pollinators is thought to be the driving force for the evolution of floral traits. Specifically in the New World (NW), the oil-bee pollination syndrome has led to the convergence of floral characters in two distantly related families of core eudicots, Malpighiaceae and Krameriaceae. Both families display a flag-like structure that establishes a zygomorphic flower and floral oil rewards in epithelial elaiophores. These traits work concomitantly to attract and reward female oil-bees that help fertilize these flowers and in return receive oils. The underlying genetics of floral zygomorphy were studied in several clades of core eudicots, …
Heuchera Alba Twitter Identification, Christopher T. Martine
Heuchera Alba Twitter Identification, Christopher T. Martine
Other Faculty Research and Publications
The casual posting of a misidentified photograph led to a tweet exchange among Chris Martine, Bucknell University; Ryan Folk, Florida Museum of Natural History; and Scott Schuette, Pennsylvania Natural Heritage Program in June 2017. The scientists used their private accounts to conduct a discussion about the specific species identified. This exchange opened the possibility of a previously unknown range of a rare species, which Martine explored further and documented in the scientific publication PhytoKeys.
Tweet id: 873120823464218624
user ID: @MartineBotany
Related records included in this repository are "The hidden Heuchera: How science Twitter uncovered a globally imperiled species in Pennsylvania, …
Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault
Department of Agronomy and Horticulture: Faculty Publications
Transcriptomic approaches revealed thousands of genes differentially or specifically expressed during nodulation, a biological process resulting from the symbiosis between leguminous plant roots and rhizobia, atmospheric nitrogen-fixing symbiotic bacteria. Ultimately, nodulation will lead to the development of a new root organ, the nodule. Through functional genomic studies, plant transcriptomes have been used by scientists to reveal plant genes potentially controlling nodulation. However, it is important to acknowledge that the physiology, transcriptomic programs, and biochemical properties of the plant cells involved in nodulation are continuously regulated. They also differ between the different cell-types composing the nodules. To generate a more accurate …
First Case Of Glufosinate-Resistant Rigid Ryegrass (Lolium Rigidum Gaud.) In Greece, Ilias S. Travlos, Nikolina Cheimona, Rafael De Prado, Amit J. Jhala, Demosthenis Chachalis, Eleni Tani
First Case Of Glufosinate-Resistant Rigid Ryegrass (Lolium Rigidum Gaud.) In Greece, Ilias S. Travlos, Nikolina Cheimona, Rafael De Prado, Amit J. Jhala, Demosthenis Chachalis, Eleni Tani
Department of Agronomy and Horticulture: Faculty Publications
Repeated applications of the same herbicide(s), which are characterized by the same mode of action, increase selection pressure, which in turn favours the evolution of herbicide-resistant weeds. Glufosinate is a broad-spectrum non-selective herbicide being used for weed control for many years around the world. Rigid ryegrass (Lolium rigidum Gaud.) is an economically important grass weed in Greece. Recent complaints by growers about control failure of rigid ryegrass with glufosinate require further investigation and have been the basis of this study. The objectives of this study were to confirm the existence of glufosinate-resistant L. rigidum in Greece and evaluate the …
Empowerment And Tech Adoption: Introducing The Treadle Pump Triggers Farmers’ Innovation In Eastern Ethiopia, Shimelis Beyene, Teshome H. Regassa, Belaineh Legesse, Martha Mamo, Tsegaye Tadesse
Empowerment And Tech Adoption: Introducing The Treadle Pump Triggers Farmers’ Innovation In Eastern Ethiopia, Shimelis Beyene, Teshome H. Regassa, Belaineh Legesse, Martha Mamo, Tsegaye Tadesse
Department of Agronomy and Horticulture: Faculty Publications
In 2013, thirty-eight treadle pumps (TPs) were installed as low-cost technology introduction for small-scale irrigation in eastern Ethiopia. This pilot project also trained six farmers on tube well excavation, as well as the installation and maintenance of pumps. In June 2015, researchers visited nine of the thirty-eight TP villages and found only two TPs functioning as originally installed. The rest were replaced with a new technology developed by the trained farmers. Farmers, empowered by training, gained more control in developing technology options tailored to local needs and conditions of their communities. Adopters of the new technology stated that the limited …
Beyond The Plot: Technology Extrapolation Domains For Scaling Out Agronomic Science, Juan I. Rattalino Edreira, Kenneth G. Cassman, Zvi Hochman, Martin K. Van Ittersum, Lenny Van Bussel, Lieven Claessens, Patricio Grassini
Beyond The Plot: Technology Extrapolation Domains For Scaling Out Agronomic Science, Juan I. Rattalino Edreira, Kenneth G. Cassman, Zvi Hochman, Martin K. Van Ittersum, Lenny Van Bussel, Lieven Claessens, Patricio Grassini
Department of Agronomy and Horticulture: Faculty Publications
Ensuring an adequate food supply in systems that protect environmental quality and conserve natural resources requires productive and resource-efficient cropping systems on existing farmland.Meeting this challenge will be difficult without a robust spatial framework that facilitates rapid evaluation and scaling-out of currently available and emerging technologies. Here we develop a global spatial framework to delineate ‘technology extrapolation domains’ based on key climate and soil factors that govern crop yields and yield stability in rainfed crop production. The proposed framework adequately represents the spatial pattern of crop yields and stability when evaluated over the data-rich US Corn Belt. It also facilitates …
Physio-Chemical And Functional Properties Of Nine Proso Millet Cultivars, Manjot Singh, Akinbode A. Adedeji, Dipak Santra
Physio-Chemical And Functional Properties Of Nine Proso Millet Cultivars, Manjot Singh, Akinbode A. Adedeji, Dipak Santra
Biosystems and Agricultural Engineering Faculty Publications
Evaluation of the postharvest properties of nine proso millet cultivars was carried out to determine their physical and engineering properties, which are very useful for designing appropriate systems for process operations such as sorting, drying, heating, cooling, and milling. Nine cultivars of proso millet comprising waxy and non-waxy types, namely Cope, Earlybird, Huntsman, Minco, Plateau, Sunrise, Rise, Dawn, and Panhandle, were obtained from the Panhandle Research and Extension Center, University of Nebraska, Scottsbluff. Results showed significant (p < 0.05) differences in their physical properties, such as sphericity, volume, bulk density, porosity, and angle of repose, which ranged from 0.86 to 0.91, from 3.94 to 5.14 mm3, from 765.49 to 809.67 kg m-3, from 42.49% to 44.20%, and from 22.98° to 25.74°, respectively. The cultivars were …
Stored Grain Pack Factor Measurements For Soybeans, Grain Sorghum, Oats, Barley, And Wheat, Rumela Bhadra, Mark E. Casada, Aaron P. Turner, Michael D. Montross, Sidney A. Thompson, Samuel G. Mcneill, Ronaldo G. Maghirang, Josephine M. Boac
Stored Grain Pack Factor Measurements For Soybeans, Grain Sorghum, Oats, Barley, And Wheat, Rumela Bhadra, Mark E. Casada, Aaron P. Turner, Michael D. Montross, Sidney A. Thompson, Samuel G. Mcneill, Ronaldo G. Maghirang, Josephine M. Boac
Biosystems and Agricultural Engineering Faculty Publications
Grain and oilseed crops stored in bins undergo compaction due to overbearing pressure of the grain inside the structure. Thus, volume measurements of grain in bins need to be combined with the amount of packing (usually called pack factor) in addition to the initial density so that the mass in the structure can be calculated. Multiple pack factor prediction methods are in use in the grain industry, but they have only been validated in the literature and compared with field data for corn and hard red winter wheat. Predictions from WPACKING, the program in ASABE Standard EP413.2, and two standard …
Morphology, Taxonomy And Mating‑Type Loci In Natural Populations Of Volvox Carteri In Taiwan, Hisayoshi Nozaki, Noriko Ueki, Mari Takusagawa, Shota Yamashita, Osami Misumi, Ryo Matsuzaki, Masanobu Kawachi, Yin-Ru Chiang, Jiunn-Tzong Wu
Morphology, Taxonomy And Mating‑Type Loci In Natural Populations Of Volvox Carteri In Taiwan, Hisayoshi Nozaki, Noriko Ueki, Mari Takusagawa, Shota Yamashita, Osami Misumi, Ryo Matsuzaki, Masanobu Kawachi, Yin-Ru Chiang, Jiunn-Tzong Wu
Publications and Research
Background: Volvox carteri f. nagariensis is a model taxon that has been studied extensively at the cellular and molecular level. The most distinctive morphological attribute of V. carteri f. nagariensis within V. carteri is the production of sexual male spheroids with only a 1:1 ratio of somatic cells to sperm packets or androgonidia (sperm packet initials). However, the morphology of male spheroids of V. carteri f. nagariensis has been examined only in Japanese strains. In addition, V. carteri f. nagariensis has heterothallic sexuality; male and female sexes are determined by the sex-determining chromosomal region or mating-type locus composed of a …
New Additions To The Flora Of San Nicolas Island, Ventura County, California, Benjamin E. Carter, William F. Hoyer Iii, Jonathan Dunn, C. Matt Guilliams
New Additions To The Flora Of San Nicolas Island, Ventura County, California, Benjamin E. Carter, William F. Hoyer Iii, Jonathan Dunn, C. Matt Guilliams
Aliso: A Journal of Systematic and Floristic Botany
Ongoing collecting efforts on San Nicolas Island have substantially increased the number of plant species documented from the island. Here we report thirty-one plants previously unrecorded from the island. The list includes six eudicots, one monocot, four liverworts and twenty mosses. Five of these species are understood to be introduced on San Nicolas and the remainder are believed to be native. The native vascular plants are Logfia filaginoides, Cistanthe maritima and Muhlenbergia microsperma. Of the twenty-four new bryophytes, one—Asterella bolanderi—is the first record from the Channel Islands. Specific ecological and locality information are provided for the …
Contents 36(2)
Aliso: A Journal of Systematic and Floristic Botany
No abstract provided.
Effects Of Red Clover Isoflavones On Tall Fescue Seed Fermentation And Microbial Populations In Vitro, Emily A. Melchior, Jason K. Smith, Liesel G. Schneider, Travis Mulliniks, Gary E. Bates, Zachary D. Mcfarlane, Michael D. Flythe, James L. Klotz, Jack P. Goodman, Huihua Ji, Phillip R. Meyer
Effects Of Red Clover Isoflavones On Tall Fescue Seed Fermentation And Microbial Populations In Vitro, Emily A. Melchior, Jason K. Smith, Liesel G. Schneider, Travis Mulliniks, Gary E. Bates, Zachary D. Mcfarlane, Michael D. Flythe, James L. Klotz, Jack P. Goodman, Huihua Ji, Phillip R. Meyer
West Central Research and Extension Center, North Platte
Negative impacts of endophyte-infected Lolium arundinaceum (Darbyshire) (tall fescue) are responsible for over $2 billion in losses to livestock producers annually. While the influence of endophyte-infected tall fescue has been studied for decades, mitigation methods have not been clearly elucidated. Isoflavones found in Trifolium pratense (red clover) have been the subject of recent research regarding tall fescue toxicosis mitigation. Therefore, the aim of this study was to determine the effect of ergovaline and red clover isoflavones on rumen microbial populations, fiber degradation, and volatile fatty acids (VFA) in an in vitro system. Using a dose of 1.10 mg …
Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 19 2018, Several Authors
Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 19 2018, Several Authors
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
No abstract provided.
Agricultural Information Needs And Food Access In The Stann Creek District Of Belize, Sam E. Harris, Donna L. Graham
Agricultural Information Needs And Food Access In The Stann Creek District Of Belize, Sam E. Harris, Donna L. Graham
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
The purpose of this study was to describe agricultural information sources available to farmers and to describe food access and availability for the people of Dangriga, Stann Creek, Belize. This study used descriptive survey research methods with convenience sampling of the general public (n=22) and of farmers (n = 38) in the summer of 2017. Farmers use a variety of agricultural information sources with the extension service cited most often, followed by friends and fellow farmers. Weather, lack of information, pests, and inadequate access to capital were of primary concern for farmers. Face-to-face meetings were used most often by extension …
Tripartite Interactions Of Legumes With Arbuscular Mycorrhizal Fungi And Rhizobial Bacteria: Insight Into Plant Growth, Seed Yield, And Resource Exchange, Arjun Kafle
Electronic Theses and Dissertations
Under natural conditions, legumes, such as alfalfa (Medicago) and soybean (Glycine max) are colonized with arbuscular mycorrhizal (AM) fungi and rhizobial bacteria forming tripartite interactions. Legumes are important crop species due to their high nutritional and economic values. Most of the previous literatures focused on experiments with an individual symbiont: either AM fungi or rhizobial bacteria, but not with both symbionts at the same time, thus our current understanding of resource exchange in tripartite interactions is limited. It has been reported that AM fungi primarily provide phosphate (P), nitrogen (N), and other nutritional and non-nutritional benefits while rhizobial bacteria solely …
Genetic Component Effects Of Two Loci On Seed Dormancy, Awn, Low-Temperature Germination, Plant Height, And Flowering Time In Rice (Oryza Sativa L.), Ugur Korkmaz
Electronic Theses and Dissertations
Seed dormancy (SD) is an adaptive trait of both ecological and agricultural importance. Cereal crops have been selected for reduced SD to promote germination, which also caused the pre-harvest sprouting (PHS) problem in crop production. The previous research identified a set of quantitative trait loci (QTL) for SD, including qSD1- 2 and qSD8, in the conspecific weedy and cultivated rice (Oryza sativa). qSD1-2 carries a dormancy-enhancing allele from the cultivated rice and has a pleiotropic effect on plant height. qSD8 carries a dormancy-enhancing allele from the weedy rice and tightly links to qAL8 for awn length. The …
Exploiting The Genetic Diversity Of Wild Ancestors And Relatives Of Wheat For Its Improvement, Jagdeep Singh Sidhu
Exploiting The Genetic Diversity Of Wild Ancestors And Relatives Of Wheat For Its Improvement, Jagdeep Singh Sidhu
Electronic Theses and Dissertations
Wheat is the third most staple food worldwide but current 1% annual improvement in the wheat production is insufficient to meet the growing demands in future. The narrow genetic base of wheat limits continuous improvement in wheat productivity and tolerance to biotic and abiotic stresses under changing climate. Wild ancestors and relatives of wheat hold a potential in widening the genetic pool of wheat and enhance its resilience to biotic and abiotic stresses. This study was focused towards characterizing the genetic diversity in wild relatives of wheat for disease resistance and efficient association with diazotrophs. In the first study, we …
Application Of Genomic Approaches To Improve Yield And Bacterial Leaf Streak Resistance In Winter Wheat, Sai Mukund Ramakrishnan
Application Of Genomic Approaches To Improve Yield And Bacterial Leaf Streak Resistance In Winter Wheat, Sai Mukund Ramakrishnan
Electronic Theses and Dissertations
Global wheat production is threatened by the change in climate thus leading lead to the increase in the biotic and abiotic stresses. We need to increase wheat productivity at a faster pace and manage these challenges to meet the growing demand. Development of cultivars with durable disease resistance and enhancing the rate of genetic gain in wheat are the major goals in wheat breeding programs. Bacterial Leaf Streak (BLS) is one of the most threatening bacterial diseases to wheat in the US Northern Great Plains. Unlike fungal diseases, bacterial diseases cannot be effectively managed using chemicals and thus developing disease …
Study Of Seasonal Change And Water Stress Condition In Plant Leaf Using Polarimetric Lidar Measurement, Prabeen Kattel
Study Of Seasonal Change And Water Stress Condition In Plant Leaf Using Polarimetric Lidar Measurement, Prabeen Kattel
Electronic Theses and Dissertations
Study of vegetation is of great importance to the improvement of agriculture and forest management. Although there have been various attempts to characterize vegetation using remote sensing techniques, polarimetric lidar is a novel remote sensing tool that has shown potential in vegetation remote sensing. In this thesis, a near-infrared polarimetric lidar at 1064 nm was used to investigate the effects of seasonal change and water stress condition on plant leaves. Two variables, time and water content, affected the plant leaf laser depolarization ratio measurement. The first study focused on the maple tree in order to figure out how seasonal change …
Air-Propelled Organic Fertilizer Grits Can Be Used To Control Weeds And Provide Nitrogen, Michael Carlson
Air-Propelled Organic Fertilizer Grits Can Be Used To Control Weeds And Provide Nitrogen, Michael Carlson
Electronic Theses and Dissertations
Weeds are one of the biggest challenges for organic growers because of the alternative weed control methods. Air-propelled abrasive grit management has been reported to control weed seedlings in corn and soybeans while maintaining yield. This research examined the weed control, corn and soybean yields, nitrogen mineralization, and nitrogen yield responses from grits. The grits used in this research included: Phytaboost Plant Food 7-1-2 (soybean meal), Sustane 8-2-4 and 4-6-4 (turkey litters), and two non-fertilizer grits: Agra Grit (walnut shells) and corn cob meal. Field studies were conducted from 2015 to 2017 in Aurora, SD, Beresford, SD and Morris, MN. …
Insecticidal Seed And In-Furrow Treatment Recommendations For Soybean And Sunflower, Brady Hauswedell
Insecticidal Seed And In-Furrow Treatment Recommendations For Soybean And Sunflower, Brady Hauswedell
Electronic Theses and Dissertations
Throughout the 2016 and 2017 growing season, field research experiments were replicated across South Dakota. Many times seed treatments are used prophylactic, which is neither good for the producers or the environment. Producers will be able to reduce production costs, if they only use a seed treatment when necessary. The purpose of the first experiment was to determine the effects of seed treatments in combination with planting date and seeding rate on soybean yield. To determine the effects, two years of field data from four eastern South Dakota locations were compared. Within each year and location we compared two planting …
Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler
Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler
Electronic Theses and Dissertations
Plant feedstuff by-products such as soy processing wastewater, guar korma meal, and sorghum hominy are very different, however, all possess a rich nutrient profile. Lessening their value is the presence of lignocellulose, plant anti-nutritional factors, and unfavorable protein profiles. Fungal conversion processes are an attractive approach to improving the value of these by-products by degrading detrimental fractions of each while simultaneously creating nutrient-rich cell mass. The aim of this research was to improve the value of each by-product for potential application in monogastric diets such as fish. Fungal organisms, both yeast and filamentous fungi, were examined for their ability to …
Winter Surveys Of Cotinus Obovatus (American Smoketree) In The Ozark Mountains, Gary R. Graves
Winter Surveys Of Cotinus Obovatus (American Smoketree) In The Ozark Mountains, Gary R. Graves
Journal of the Arkansas Academy of Science
Cotinus obovatus (American smoketree) is a rare deciduous tree with a relictual distribution in southeastern North America. Efforts to map its fine-scale geographic distribution in the Ozark Mountains have been limited to the growing season when the distinctive blooming panicles and foliage facilitate detection in hardwood-cedar woodlands. I describe the physiognomic traits of leafless C. obovatus that permit effective population mapping in winter landscapes. Clumped growth and diagonally leaning stems facilitate detection at a distance. Bark texture, twig morphology, and sap odor confirm the identity of the tree at close range.
Black Cohosh Seed Germination And Conservation, W. John Hayden
Black Cohosh Seed Germination And Conservation, W. John Hayden
Biology Faculty Publications
Like many plant enthusiasts, I have spent a considerable amount of time planting seeds. Every year I grow many vegetables—my garden always includes some annual bedding plants—and I sow seeds of cover crops (winter wheat, winter rye, and buckwheat) by the tens of thousands. While I have committed vast numbers of propagules to moist soil, I cannot say that I have watched every single one sprout. Nevertheless, I certainly have observed the germination process many, many times for lots of different seeds. For these seeds of garden plants, germination is quite rapid, just a few days to maybe as much …
Characterization Of Neofabraea Actinidiae And N. Brasiliensis As Causal Agents Of Apple Bull’S-Eye Rot In Southern Brazil, Amauri Bogo, Carla C. Comparin, Rosa M. Valdebenito Sanhueza, Patricia Ritschel, Ricardo T. Casa, Fábio N. Silva, Sydney E. Everhart
Characterization Of Neofabraea Actinidiae And N. Brasiliensis As Causal Agents Of Apple Bull’S-Eye Rot In Southern Brazil, Amauri Bogo, Carla C. Comparin, Rosa M. Valdebenito Sanhueza, Patricia Ritschel, Ricardo T. Casa, Fábio N. Silva, Sydney E. Everhart
Department of Plant Pathology: Faculty Publications
The causal agents of apple bull’s-eye rot in southern Brazil have recently been described as Neofabraea actinidiae and N. brasiliensis. Isolates of both species were evaluated for response of mycelial growth index (MGI) to different temperatures, enzyme production, mycelial growth inhibition and effective concentrations (EC50 and EC100) of the fungicides triflumizole, pyrimethanil and thiophanate methyl, as well as aggressiveness on fruits of ‘Fuji’ hybrid and ‘Pink Lady’. There was significantly lower mycelium growth in N. brasiliensis compared with N. actinidiae at all temperatures tested. Neither species grew at 3 and 32°C. There were minor differences in …
Elucidating The Role Of Highly Homologous Nicotiana Benthamiana Ubiquitin E2 Gene Family Members In Plant Immunity Through An Improved Virus‑Induced Gene Silencing Approach, Bangjun Zhou, Lirong Zeng
Elucidating The Role Of Highly Homologous Nicotiana Benthamiana Ubiquitin E2 Gene Family Members In Plant Immunity Through An Improved Virus‑Induced Gene Silencing Approach, Bangjun Zhou, Lirong Zeng
Department of Plant Pathology: Faculty Publications
Background: Virus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a high-throughput manner. However, the specificity and efficiency for knocking down members of a highly homologous gene family have remained to date a significant challenge in VIGS due to silencing of off-targets. Results: Here we present an improved method for the selection and evaluation of gene fragments used for VIGS to specifically and efficiently knock down members of a highly homologous gene family. Using this method, we knocked down …
The Tomato U-Box Type E3 Ligase Pub13 Acts With Group Iii Ubiquitin E2 Enzymes To Modulate Fls2-Mediated Immune Signaling, Bangjun Zhou, Lirong Zeng
The Tomato U-Box Type E3 Ligase Pub13 Acts With Group Iii Ubiquitin E2 Enzymes To Modulate Fls2-Mediated Immune Signaling, Bangjun Zhou, Lirong Zeng
Department of Plant Pathology: Faculty Publications
In Arabidopsis and rice, the ubiquitin ligase PUB13-mediated protein degradation plays a significant role in plant pattern-triggered immunity (PTI) and flowering time control. The Arabidopsis PUB13 has been shown to attenuate the pattern recognition receptor FLS2-mediated immune signaling by ubiquitinating FLS2 and consequently promoting its degradation by the 26S proteasome. Nevertheless, the cognate ubiquitin-conjugating enzymes (E2) with which PUB13 acts to modulate FLS2-mediated PTI are unknown. To address this question, we investigate here the tomato (Solanum lycopersicum) homolog of PUB13, SlPUB13 by utilizing the recently characterized complete set of tomato E2s. Of the 13 groups of tomato E2s, …
Proteomic Analysis Of Ubiquitinated Proteins In Rice (Oryza Sativa) After Treatment With Pathogen-Associated Molecular Pattern (Pamp) Elicitors, Xiao-Lin Chen, Xin Xie, Liye Wu, Caiyun Liu, Lirong Zeng, Xueping Zhou, Feng Luo, Guo-Liang Wang, Wende Liu
Proteomic Analysis Of Ubiquitinated Proteins In Rice (Oryza Sativa) After Treatment With Pathogen-Associated Molecular Pattern (Pamp) Elicitors, Xiao-Lin Chen, Xin Xie, Liye Wu, Caiyun Liu, Lirong Zeng, Xueping Zhou, Feng Luo, Guo-Liang Wang, Wende Liu
Department of Plant Pathology: Faculty Publications
Reversible protein ubiquitination plays essential roles in regulating cellular processes. Although many reports have described the functions of ubiquitination in plant defense responses, few have focused on global changes in the ubiquitome. To better understand the regulatory roles of ubiquitination in rice pattern-triggered immunity (PTI), we investigated the ubiquitome of rice seedlings after treatment with two pathogen-associated molecular patterns, the fungal-derived chitin or the bacterialderived flg22, using label-free quantitative proteomics. In chitin-treated samples, 144 and 167 lysine-ubiquitination sites in 121 and 162 proteins showed increased and decreased ubiquitination, respectively. In flg22-treated samples, 151 and 179 lysine-ubiquitination sites in 118 and …