Digital Commons Network^™

Biotinylation Of K8 And K12 Co-Occurs With Acetylation And Mono-Methylation In Human Histone H4, Yap Ching Chew, Ashraf S. Raza, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Histones H1, H2A, H2B, H3, and H4 are proteins that are critical for folding of DNA into chromatin. Posttranslational acetylation, methylation, and biotinylation of histones participate in gene silencing, mitotic condensation of chromatin, and the cellular response to DNA damage. Various modifications of histones are known to interact ("cross-talk") in chromatin-remodeling events; interactions may be synergistic or antagonistic. Here, we sought to identify biotinylation sites in human histone H4 by using mass spectrometry (MS/MS), and we sought to determine whether biotinylation co-exists with acetylation and methylation in the same H4 molecule. Nuclear histones from human lymphoid (Jurkat) cells were digested …

Anthropogenic Drivers Of Ecosystem Change: An Overview, Gerald C. Nelson, Elena Bennett, Asmeret A. Berhe, Kenneth G. Cassman, Ruth Defries, Thomas Dietz, Achim R. Dobermann, Andrew Dobson, Anthony Janetos, Marc Levy, Diana Marco, Nebojsa Nakicenovic, Brian O'Neill, Richard Norgaard, Gerhard Petschel-Held, Dennis Ojima, Prabhu Pingali, Robert Watson, Monika Zurek

Department of Agronomy and Horticulture: Faculty Publications

This paper provides an overview of what the Millennium Ecosystem Assessment (MA) calls “indirect and direct drivers” of change in ecosystem services at a global level. The MA definition of a driver is any natural or human-induced factor that directly or indirectly causes a change in an ecosystem. A direct driver unequivocally influences ecosystem processes. An indirect driver operates more diffusely by altering one or more direct drivers. Global driving forces are categorized as demographic, economic, sociopolitical, cultural and religious, scientific and technological, and physical and biological. Drivers in all categories other than physical and biological are considered indirect. Important …

Ethylene Could Influence Ferric Reductase, Iron Transporter, And H+-Atpase Gene Expression By Affecting Fer (Or Fer-Like) Gene Activity, Carlos Lucera, Brian M. Waters, Francisco Javier Romera, María José García, María Morales, Esteban Alcántara, Rafael Pérez-Vicente

Department of Agronomy and Horticulture: Faculty Publications

In previous works, it has been shown, by using ethylene inhibitors and precursors, that ethylene could participate in the regulation of the enhanced ferric reductase activity of Fe-deficient Strategy I plants. However, it was not known whether ethylene regulates the ferric reductase gene expression or other aspects related to this activity. This paper is a study of the effects of ethylene inhibitors and precursors on the expression of the genes encoding the ferric reductases and iron transporters of Arabidopsis thaliana (FRO2 and IRT1) and Lycopersicon esculentum (= Solanum lycopersicum) (FRO1 and IRT1) plants. The effects …

Nutrient Uptake Of Maize Affected By Nitrogen And Potassium Fertility In A Humid Subtropical Environment, H. Arnold Bruns, M. Wayne Ebelhar

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen (N) and potassium (K) fertility management of maize (Zea mays L.) in the humid subtropical Mississippi Delta may differ from a temperate climate because of its use in rotation with cotton (Gossypium hirsutum L.), soil temperatures rarely falling to 0 °C, and heavy winter rains that facilitate nutrient losses. An experiment to determine the [N] (concentration = [ ]), phosphorus [P], [K], calcium [Ca], magnesium [Mg], iron [Fe], manganese [Mn], zinc [Zn], and copper [Cu] and their total contents plant^-1 of maize grown in rotation with cotton, using N fertility levels of (134, 179, 224, 269, …

International Shipping Of Fumonisins From Maize Extracts On C18 Sorbent, R. T. Riley, O. A. Torres, E. Palencia

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fumonisins are mycotoxins found in maize. In developing countries, the resources required for analysis are often lacking, and the shipping of maize between countries can be difficult since the importation of plant materials requires permits/ inspection to prevent the entry of pests that frequently infest maize. A simple, safe and legal method for shipping maize extracts to the USA was needed to conduct a survey of fumonisins in Central America. The objective was to develop a method for isolating and shipping maize extracts for fumonisin analysis so as to facilitate a survey of fumonisin exposure. The results indicate that fumonisins …

Wayne E. Sabbe Arkansas Soil Fertility Studies 2005, Nathan A. Slaton

Arkansas Agricultural Experiment Station Research Series

Rapid technological changes in crop management and production require that research efforts be presented in an expeditious manner. The contributions of soil fertility and fertilizers are major production factors in all Arkansas crops. The studies described within will allow producers to compare their practices with the university’s research efforts. Additionally, soil test data and fertilizer sales are presented to allow comparisons among years, crops, and other areas within Arkansas.

Determination Of The Primary Levels Of The Residues Of The Seeds Of Wheat, Maize, And Barley For The Elaboration Of The Food Mixture For Rabbits, Aida Esperanza Morejon Jacome

Theses and Dissertations

The present investigation was done in the parish of Chaltura, Granja "La Pradera" which belongs to the University Técnica del Norte. The main objective of this investigation was to determine the primary levels of the residues of the seeds of wheat, maize, barley for the elaboration of the food mixture for rabbits. The evaluated variables, food consumed, weight gain, food value, protein percentage, production cost per treatment. Previously to the initiation of the field phase bromatological analysis were done on the three food mixtures in the National Institute of Farming Investigation, Quality and Nutrition Area. The treatments were four: TI …

The Effect Of Nitrogen Fertilizer Application To Maize And Sorghum On The Bionomics Of Chilo Partellus (Lepidoptera: Crambidae) And The Performance Of Its Larval Parasitoid Cotesia Flavipes (Hymenoptera: Braconidae), N. Jiang, F. Schulthess

All Peer-Reviewed Publications

Laboratory and field trials were conducted to evaluate the effect of plant species (maize, sorghum), plant age (young, middle, old) and four different nitrogen fertilization levels (N0-N3) on the bionomics of the invasive crambid Chilo partellus and the performance of its braconid larval parasitoid Cotesia flavipes. Plant N varied significantly between N0 and N1-N3, but the differences among the latter were not significant. Intrinsic rates of increase and net-reproductive rates of C. partellus followed the same trends: they were lowest with N0 and similar among the other treatments. On maize only, mortality of C. partellus and parasitism by C. flavipes …

Effects Of Common Forage Phenolic Acids On Escherichia Coli O157:H7 Viability In Bovine Feces, J. E. Wells, E. D. Berry, V.H. Varel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ruminant animals are carriers of Escherichia coli O157:H7, and the transmission of E. coli O157:H7 from cattle to the environment and to humans is a concern. It is unclear if diet can influence the survivability of E. coliO157:H7 in the gastrointestinal system or in feces in the environment. Feces from cattle fed bromegrass hay or corn silage diets were inoculated with E. coliO157:H7, and the survival of this pathogen was analyzed. When animals consumed bromegrass hay for <1 month, viable E. coliO157:H7 was not recovered after 28 days postinoculation, but when animals consumed the diet for >1 month, E. coli …

Production And Handling Practices For Safe Produce, Pamela L. Brady, Justin R. Morris

AAES Research Reports and Research Bulletins

The University of Arkansas Division of Agriculture received a grant from the USDA’s Initiative for Future Agriculture and Food Systems program to help small- and medium-sized farms become more profitable and to therefore add stability to the family farm. One approach to doing this is to help farmers growing produce reduce or eliminate safety hazards which may be associated with their products.

Nebline, November/December 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Community Food Programs Help Limited-Resource Families Eat Healthy
NEP Teaches People How to Stretch Their Food Dollar
Get Involved in the Fight Against Hunger
Free Soybean Cyst Nematode Sample Kits Available
Don’t Forget to Winterize Your Underground Sprinkler System
Agricultural Tax Planning for 2005
Nebraska Arboretum Speakers Bureau
Setting Goals for Your Small Farm
Prepare Horses For Winter
Improve Nebraska Woodlands in Late Fall and Winter
“Woody Florals” Acreage Insights Clinic, Nov. 10
Prevent Soil Erosion
Thanksgiving — the Tradition Continues
Ginger Citrus Rice Recipe
FREE Healthy Desserts Presentation, Nov. 15
$tretch Your Food Dollar with Leftover Turkey
President’s Notes …

Production Techniques For Crimson Seedless Table Grapes In Western Australia, Ian Cameron

Viticulture published reports

Crimson Seedless is a late season, red seedless table grape developed by D. Ramming and R. Tarailo in Fresno California for the United States Department of Agriculture. It was bred in 1979 when a white, late ripening seedless hybrid variety was crossed with Red Emperor (see Figure 1). The seedling, which became Crimson Seedless, was selected in 1983 but not released to industry in California until 1989.

Production techniques for Crimson Seedless were largely unknown when the variety was released to industry in Western Australia in 1996. Specific management techniques suitable for Californian conditions had been described by various authors. …

Corn Stalk Nitrate Concentration Profile, W. Wilhelm, G. E. Varvel, J. S. Schepers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The end-of-season nitrate test provides a method of assessing the N available to the corn (Zea mays L.) crop during the latter part of the season. This study was conducted to determine how stalk nitrate test results and interpretations are affected by sample composition. Stalks were collected from three filed sites and separated into phytomers (node plus internode above), which were subdivided into three or five segments after length was measured. Nitrate-N concentration of phytomers decreased linearly from the soil to the ear. Within a phytomer, segments also decreased acropetally (from base to apex). Node tissue NO₃-N …

Corn Stalk Nitrate Concentration Profile, Wally Wilhelm, Gary E. Varvel, James S. Schepers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The end-of-season stalk nitrate test provides a method of assessing the N available to the corn (Zea mays L.) crop during the latter part of the season. This study was conducted to determine how stalk nitrate test results and interpretations are affected by sample composition. Stalks were collected from three field sites and separated into phytomers (node plus internode above), which were subdivided into three or five segments after length was measured. Nitrate-N concentration of phytomers decreased linearly from the soil to the ear. Within a phytomer, segments also decreased acropetally (from base to apex). Node tissue NO_{3 …}

Nebraska Vine Lines, Volume Viii, Issue 5, October/November 2005

Nebraska Vine Lines (2003–2006)

• Highlights of The North American Grape Breeders Roundtable

• Paul Read to participate in Facultry Development Leave at the University of Tasmania

• Grape Breeding 101

• Small Scale Commercial Wine Making Workshop

• University Research Plots by Steve Gamet

• Fermenter’s Supply and Equipment

• Be Sure to put the 9th Annual Forum on your calendar

• The Japanese Beetle by Chelsey Wasem

• Reams Sprinkler Supply

Nebline, October 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Students Prepare for Careers at UNL College of Ag and Natural Resources
CASNR’s “Ensuring Your Future Guarantee”
Check Out UNL and CASNR
Drying Gourds
Deep Watering Trees in Fall can Prevent Winter Drying Injury
Gladiola Fall Care
Storing Vegetables
Farmer Question: How Can I Reduce Energy Costs When Drying Grain?
Avoid Pasture Damage During Fall Grazing
Food Safety 101: USDA Offers Tips for College Students
One-Dish Rosemary Chicken and White Beans Recipe
Healthy Desserts Presentation, Nov. 15
Breakfast Power
How Healthy is Your Home? Be Aware of Indoor Air Pollutants
President’s Notes — Janet’s Jargon
FCE News & Events
Household …

La Flore Qui A Faim : Une Enquête Chimique, Biologique Et Culturelle De La Dimorphisme Rouge/Verte Et Les Applications Médicinales De Nepenthes Madagascariensis, Keirnan Willett

Independent Study Project (ISP) Collection

Le Nepenthes madagascariensis est une plante carnivore endémique du côté sud-est de Madagascar qui attirent la proie arthropode (insectes, araignées etc.) en employant les bouts de feuilles convertis en forme des pichets ou urnes. Après d’être attiré par le suc au bord de l’urne, les insectes devint désorientés, perdent la traction et se coulent dans le liquide riche en enzymes digestifs au dessous (14). Les substances nutritives des cadavres sont absorbées par la plante en compensant pour la lacune d’azote dans le sol. Les couvercles des urnes expriment une couleur rouge ou verte. Notre étude examine les différences chimiques et …

Relationships Of Intercropped Maize, Stem Borer Damage To Maize Yield And Land-Use Efficiency In The Humid Forest Of Cameroon, A. Chabi-Olaye, C. Nolte, F. Schulthess, C. Borgemeister

All Peer-Reviewed Publications

Stem borers are the most important maize pests in the humid forest zone of Cameroon. Field trials were conducted in the long and short rainy seasons of 2002 and 2003 to assess the level of damage and yield reductions caused by stem borers in monocropped maize and in maize intercropped with non-host plants such as cassava, cowpea and soybean. The intercrops were planted in two spatial arrangements, i.e. alternating hills or alternating rows. All intercrops and the maize monocrop were grown with and without insecticide treatment for assessment of maize yield loss due to borer attacks. The land-use efficiency of …

Biotinylation Of K12 In Histone H4 Decreases In Response To Dna Double-Strand Breaks In Human Jar Choriocarcinoma Cells1,2, Nagarama Kothapalli, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We tested the hypothesis that biotinylation of K12 in histone H4 plays a role in the cellular response to double-strand breaks (DSB) of DNA in human cells. DSB were caused by treating choriocarcinoma JAr cells with etoposide. Biotinylation of K12 in histone H4 decreased by 50% as early as 10–20 min after initiation of treatment with etoposide. Biotinylation returned to initial levels 30–40 min after the addition of etoposide to the medium. Temporal patterns of K12-biotinylation were similar for human lymphoma cells. Phosphorylation of S14 of histone H2B and poly(ADP-ribosylation) of glutamate residues on histone H2A are known markers of …

Effects Of Summer Grazing Strategies On Organic Reserves And Root Characteristics Of Big Bluestem, E. M. Mousel, Walter H. Schacht, C. W. Zanner, Lowell E. Moser

Department of Agronomy and Horticulture: Faculty Publications

Quantifying root structure response to multiple defoliation events in a grazing situation is critical in developing management plans for warm-season tall grasses. A pasture experiment was conducted in 1999, 2000, and 2001 near Mead, NE. The objective of the experiment was to determine the effect of timing and frequency of grazing on big blue- stem (Andropogon gerardii Vitman) etiolated tiller growth and root and rhizome structure. Paddocks were grazed at a stocking rate of 9.9 Animal Unit Month (AUM) ha^-1 in two to four cycles from mid-May to early-September. In April 2002, five 6.6- 132-cm soil cores were …

Composition And Methods Of Using The Mirabilis Mosaic Caulimovirus Sub-Genomic Transcript (Sgt) Promoter For Plant Genetic Engineering, Indu B. Maiti, Nrisingha Dey

Kentucky Tobacco Research and Development Center Faculty Patents

The isolation of and methods of using a sub-genomic transcript (Sgt) promoter from Mirabilis mosaic virus (MMV) are described. A 333 bp MMV Sgt promoter fragment (sequence−306 to +27 from the transcription start site, TSS) was found to be sufficient for strongest promoter activity. This MMV Sgt promoter fragment shows comparable promoter activity to the MMV FLt promoter both in transgenic plants and in protoplasts. The MMV Sgt promoter also demonstrates much greater activity compared to Cauliflower mosaic virus (CaMV) 19S promoter and 35S promoter. The MMV Sgt promoter fragment and any chimeric gene to which it may be linked …

K4, K9 And K18 In Human Histone H3 Are Targets For Biotinylation By Biotinidase, Kenya A. Kobza, Gabriela Camporeale, Brian Rueckert, Alice Kueh, Jacob B. Griffin, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Histones are modified post-translationally, e.g. by methylation of lysine and arginine residues, and by phosphorylation of serine residues. These modifications regulate processes such as gene expression, DNA repair, and mitosis and meiosis. Recently, evidence has been provided that histones are also modified by covalent binding of the vitamin biotin. The aims of this study were to identify biotinylation sites in histone H3, and to investigate the crosstalk among histone biotinylation, methylation and phosphorylation. Synthetic peptides based on the sequence of human histone H3 were used as substrates for enzymatic biotinylation by biotinidase; biotin in peptides was probed using streptavidin peroxidase. …

Nebraska Vine Lines, Volume Viii, Issue 4. August/September 2005.

Nebraska Vine Lines (2003–2006)

• Imad Dami to speak at November 5th Workshop

• Cutting Edge Cultivars: A Report on the Eastern Region ASEV Meeting by Paul E. Read

• Grape Crown Gall Biology and Strategies for Control by Thomas J. Burr

• Fermenter’s Supply and Equipment

• Interdisciplinary Research Program by Chelsey Wasem

• Nebraska Vine Lines by E-mail

• Be sure to put the 9th Annual Forum on your calendar

• Multi-colored Asian Lady Beetles: Good guys with some bad habits by Dr. Frederick Baxendale

• Insect Spotlight: Grape Phylloxera

• Apple Flea Beetles found in Ogallala by Chelsey Wasem

Nebline, August 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

4-H Food Booth at County Fair: Volunteers Have Fun, Rewarding Experience
Support 4-H by Supporting the 4-H Corner Stop
Booth Funds 4-H Council Activities
Household Hints: Energy Saving Ideas
  President’s Notes — Janet’s Jargon
Praise and Encouragement
2005 Scholarship Winner
Pack Your Street Sense When You Pack Your Bags
New Effective Mosquito Repellents Less Greasy, Odor Free
Control Mosquito Populations
Scrap Tire Collection Sept. 24 & 25
Using Natural Enemies to Control Pests
Some Beneficial Predators
Soybean Management Field Day, Aug. 12
Plant Alfalfa in August
St. Johnswort in Lancaster County
Free Soybean Cyst Nematode Sample Kits Available
Ammoniate Wheat …

B.R. Wells Rice Research Studies 2004, R. J. Norman, J. F. Meullenet, K. A.K. Moldenhauer

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Sheep Updates 2005 - Part 6, Sarah Pugh, Giles Glasson, Ed Barrett-Lennard, Hayley Norman, Matt Wilmot, Meir Altman, Kelly Pearce, Sally Phelan, David Masters, Nadine Eva, Brad Wooldridge, Stephen Gherardi, Lucy Anderton, Gonzalo Mata, P. Sanford, J Young, Tim Wiley, Richard Quinlan, Chris Oldham, Mike Hyder, Mandy Curnow, Samantha Giles, Andrew Thompson

Sheep Updates

This session covers seven papers from different authors:

PASTURES/GRAZING

1. New annual pastures - quality and quantity for fodder conservation?, Sarah Pugh and Giles Glasson, Department of Agriculture Western Australia

2. Saltland Pastures: Dispelling some Myths, Ed Barrett-Lennard^1,3, Hayley Norman^2,3, Matt Wilmat^2,3, Meir Altman,³, Kelly Pearce^2,3, Sally Phelan⁴, David Masters^2,3, ^1. Department of Agriculture, Western Australia, ² CSIRO Livestock Industries, Floreat, WA, ³. CRC for Plant-based Management of Dryland Salinity ⁴. Saltland Pastures Association

3. Pastures: Putting profit back into sandplain, Nadine Eva, Department …

Sheep Updates 2005 - Part 3, Rob Davidson, David Pethick, C. F. Engelke, B. D. Siebert, K. Gregg, A-D G. Wright, P. E. Vercoe, Robin Jacob, Russell Barnett, Joanne Sneddon, Sandra Brown, G. Rose, C. Kabore, J Dart, Stuart Adams

Sheep Updates

This session covers seven papers from different authors: CUSTOMER 1. Benefits VIAscanR to producers and WAMMCO, Rob Davidson, Supply Development Manager, David Pethick, School of Veterinary and Biomedical Studies, Murdock University. 2. Healthy fats in lamb: how WA lambs compare with others, C. F. Engelke Animal Biology, University of Western Australia, bCSIRO Livestock Industries, Western Australia B.D. Siebert, Department of Animal Science, University of Adelaide, South Australia, K. Gregg, Centre for High-Throughput Agricultural Genetic Analysis, Murdoch University, Western Australia. A-D.G. Wright CSIRO Livestock Industries, Western Australia, P.E Vercoe Animal Biology, University of Western Australia 3. Shelf life of fresh lamb …

Spatial Variation Of Protein, Oil, And Starch In Corn, Samuel G. Mcneill, Michael D. Montross, Scott A. Shearer

Biosystems and Agricultural Engineering Faculty Publications

Significant spatial yield variations are known to exist in cornfields with different soil types, topsoil depth, and other variables. Similarly, variations might also be found among the highly valued chemical components (oil, protein, and starch) in corn kernels due to local differences in soil type, fertility, acidity/pH, organic matter, etc. This study quantified the spatial variability of protein, oil, and starch content of corn from two conventional cornfields and two high-oil cornfields. Whole ears were harvested by hand from 20 to 40 randomly selected locations within each field. A differential global positioning system (DGPS) receiver recorded the location of each …

Nebline, July 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Avoid Portion Distortion With MyPyramid’s Specific Guidelines
Mandevilla — Tropical Vine with Showy Flowers
Fragrance in the Garden
Garden Guide: Things to do this month
Most Leaf Galls Don’t Harm Trees
Traveling This Summer? Find Bedbugs in Hotel Rooms Before They Find You!
Household Hazardous Waste Collections, June 24 & 25
Some Insects Thrive on Rat Poison
P3 Intern Working at Extension
Pine Wilt Disease
How to Sample for Pinewood Nematode
Pine Species to Avoid Planting
Presentation on Sustainable Agriculture, July 8
Predicting the Last Irrigation for Corn, Milo and Soybeans
Irrigating Summer Turf
In Hot Weather, Raise Mower Height …

Horticultural Classification Of Fruits, Larry A. Sagers

All Archived Publications

No abstract provided.