Digital Commons Network^™

A Functional Calcium-Transporting Atpase Encoded By Chlorella Viruses, Maria Cristina Bonza, Holger Martin, Ming Kang, Gentry L. Lewis, Timo Greiner, Sonia Giacometti, James L. Van Etten, Maria Ida De Michelis, Gerhard Thiel, Anna Moroni

James Van Etten Publications

Calcium-transporting ATPases (Ca²⁺ pumps) are major players in maintaining calcium homeostasis in the cell and have been detected in all cellular organisms. Here, we report the identification of two putative Ca²⁺ pumps, M535L and C785L, encoded by chlorella viruses MT325 and AR158, respectively, and the functional characterization of M535L. Phylogenetic and sequence analyses place the viral proteins in group IIB of P-type ATPases even though they lack a typical feature of this class, a calmodulin-binding domain. A Ca²⁺ pump gene is present in 45 of 47 viruses tested and is transcribed during virus infection. Complementation analysis of …

Identification Of An L-Rhamnose Synthetic Pathway In Two Nucleocytoplasmic Large Dna Viruses, Madhu Parakkottil Chothi, Garry A. Duncan, Andrea Armirotti, Chantal Abergel, James R. Gurnon, James L. Van Etten, Cinzia Bernardi, Gianluca Damonte, Michela Tonetti

James Van Etten Publications

Nucleocytoplasmic large DNA viruses (NCLDVs) are characterized by large genomes that often encode proteins not commonly found in viruses. Two species in this group are Acanthocystis turfacea chlorella virus 1 (ATCV-1) (family Phycodnaviridae, genus Chlorovirus) and Acanthamoeba polyphaga mimivirus (family Mimiviridae), commonly known as mimivirus. ATCV-1 and other chlorovirus members encode enzymes involved in the synthesis and glycosylation of their structural proteins. In this study, we identified and characterized three enzymes responsible for the synthesis of the sugar L-rhamnose: two UDP-D-glucose 4,6-dehydratases (UGDs) encoded by ATCV-1 and mimivirus and a bifunctional UDP-4-keto-6-deoxy-D-glucose epimerase/reductase (UGER) from mimivirus. Phylogenetic …

A Comparison Of Costs And Returns For Organic And Conventional Cabernet Sauvignon Grape Production In San Luis Obispo County, Amanda N. Johansen

Agribusiness

This study examined the differences in costs and profitability for established conventional and organic Cabernet Sauvignon vineyards in the Edna Valley region of San Luis Obispo County, California. The study used a partial budgeting approach to compare cost and profitability for established vineyards for 2009, and thus ignored transition costs. Data were obtained from UC Davis cost studies for organic and conventional grape production in other regions of California in previous years. Adjustments made to these costs to account for changes in input use, input costs and grape prices in San Luis Obispo County during the 2009 growing season.

The …

Greening The Green Revolution: Moving Beyond Chemicals To Sustainable Agricultural Practices, Elizabeth G. Crawley

Electronic Theses and Dissertations

Agriculture has dominated the economy of many developing countries for centuries, which has led to development policies that center on agricultural development. An obvious approach to improved production is to decrease agricultural losses in the fields and increase efficiency to markets. This study focuses on the developmental role of pesticides and chemical fertilizers on non-traditional agricultural export farming and the adoption of IPM techniques. With farmers in the Comayagua Valley of Honduras, an Asian vegetable production center for the country, as the study population, the costs and benefits of planting oriental vegetables was examined. Statistical analysis was used to examine …

The Use Of Horticulture And Gardening As A Special Education Tool At The High School Level, Krysta Marie Johnson

Horticulture and Crop Science

THIS REPORT CONTAINS PLANNING METHODS AND INSTALLATION PROCEDURE FOR A RAISED VEGETABLE GARDEN FOR WILSONVILLE HIGH SCHOOL, IN WILSONVILLE, OREGON. THE PRIMARY PURPOSE OF THE GARDEN WAS TO USE IT AS A LEARNING TOOL FOR THE SPECIAL EDUCATION DEPARTMENT AT WILSONVILLE HIGH SCHOOL. UPON DOING MORE RESEARCH IT BECAME EVIDENT THAT THE GARDEN CAN ALSO BE USED AS A HEALING TOOL FOR STUDENTS WHO SUFFER FROM ATTENTION DISORDERS AND WHO HAVE EMOTIONAL AND LEARNING DISABILITIES. THE STUDENTS HAVE BECOME INVESTED IN THE GARDEN PROJECT AND LOOK FORWARD TO DOING MORE PROJECTS WITH THEIR CLASS.

Nebline, June 2010

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 12 Reasons for Visiting a Farmers' Market

Farm Views

Horticulture

Food & Fitness

Home & Family Living

Environmental Focus

Urban Agriculture

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Cattle Grazing Reduces Survival And Reproduction Of The Western Prairie Fringed Orchid, Bonnie Warner Alexander, Donald Kirby, Mario Biondini, Edward Dekeyser

The Prairie Naturalist

QuantifYing impacts of livestock grazing and prairie management strategies on the threatened western prairie' fringed orchid (Platanthera praeclara) is difficult due to the erratic appearance of the orchid above-ground. We monitored above-ground survival of orchids from flowering to mature seed capsule production, comparing plant height, flower numbers, and seed capsule numbers from 2002-2004 in rotationally grazed pastures and non-grazed sites. Orchid survival differed significantly between grazed and non-grazed pastures, with the proportion of plants surviving from flower to capsule production consistently lower in grazed pastures. Mean orchid survival in grazed and non-grazed areas was 40% and 87%, respectively. …

The Prairie Naturalist, Volume 42, No. 1/2 June 2010, The Great Plains Natural Science Society

The Prairie Naturalist

CHANNEL CATFISH DIETS INCLUDE SUBSTANTIAL VEGETATION IN A MISSOURI RIVER RESERVOIR • J. D. Dagel, M. R. Wuellner, and D. W. Willis

USE OF LATE SEASON STANDING CORN BY FEMALE WHITE-TAILED DEER IN THE NORTHERN GREAT PLAINS DURING A MILD WINTER • T. W. Grovenburg, C. N. Jacques, C. C. Swanson, R. W. Klaver, and J. A. Jenks

COMPARISON OF TWO ZOOPLANKTON SAMPLING GEARS IN SHALLOW, HOMOGENOUS LAKES • M. E. Livings, C. W. Schoenebeck, and M. L. Brown

VEGETATION TRENDS ON A WASTE ROCK REPOSITORY CAP IN THE NORTHERN BLACK HILLS • A. C. Korth, G. E. Larson, L. …

Channel Catfish Diets Include Substantial Vegetation In A Missouri River Reservoir, Jonah D. Dagel, Melissa R. Wuellner, David W. Willis

The Prairie Naturalist

Channel catfish (Ictalurus punctatus) are native to Lake Sharpe, a Missouri River mainstem reservoir, and are common in angler catches. Channel catfish growth has declined since the formation of the reservoir in 1963. Mean lengths at time of capture for channel catfish ages 9, 10, II, and 12 have decreased by 69, 55, 115, and 21S mm, respectively, since impoundment. The objective of this study was to document monthly food habits of channel catfish throughout the growing season (May-August) in Lake Sharpe to assess potential effects of diet on growth. Although channel catfish consumed both macro invertebrates and …

Observations Of Little Blue Herons Nesting In North Dakota, And An Instance Of Probable Natural Hybridization Between A Little Blue Heron And A Cattle Egret, Alisa J. Bartos, Lawrence Igl, Marsha A. Sovada

The Prairie Naturalist

The little blue heron (Egretta caerulea) is native to North America and most commonly breeds along the coast of the southeastern United States and the Gulf of Mexico through Central America and into South America (Rodgers and Smith 1995). In North America, little blue herons rarely nest outside their coastal range. However, nesting has been documented at several locations in the northern plains including Brown, Kingsbury, and Charles Mix counties, South Dakota (Naugle et al. 1996, Tallman et al. 2002); Pope County, Minnesota (Green and Janssen 1975); and possibly in southeastern Saskatchewan (Nero and Lein 1971, Smith et al. 1996). …

Bat Survey Along The Missouri River In Central South Dakota, Alyssa M. Kiesow, Joshua A. Kiesow

The Prairie Naturalist

ats are efficient predators of night-flying insects (Whitaker 1993), particularly in urban, agricultural, and forested areas in South Dakota (Kiesow 2004). In South Dakota, 6 bat species are considered rare and presently monitored by the South Dakota Natural Heritage Program (SDNHP; South Dakota Natural Heritage Program 2002). Because bats serve a vital ecosystem function there is an increased need to conserve bats and their habitats. Hence, the objectives of this project were to determine bat species richness along the Missouri River in central South Dakota. We conducted surveys of bats using mist-nets and acoustic detection from early May to early …

Use Of Late Season Standing Corn By Female White-Tailed Deer In The Northern Great Plains During A Mild Winter, Troy W. Grovenburg, Christopher N. Jacques, Christopher C. Swanson, Robert W. Klaver, Jonathan Jenks

The Prairie Naturalist

Winter habitat and resource use of white-tailed deer (Odocoileus virginianus) have been studied extensively throughout their northern range. However, limited information exists on deer use of late season standing corn. We evaluated standing corn use by female white-tailed deer on winter range in north-central South Dakota during winter 2005-2006. Results indicate that cover type selection occurred at the population (P < 0.001) and home range (P < 0.001) levels. PopUlation level analysis indicated selection for standing corn (vi> = 4.31) and Conservation Reserve Program (CRP) grasslands (vi> = 2.81). Similarly, at the home range level, deer selected for standing corn (vi> = 1.35) and CRP grasslands (vi> = 1.44). Deer disproportionately increased use of standing corn and CRP …

Forage News [2010-06], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Update
• U.S. Supreme Court Reviews Ban on Roundup Ready® Alfalfa
• Increase Pasture Profits Using Cross Fences
• Plant Growth Regulator to Increase Pasture Growth
• Alfalfa Cutting Height to Maximize Forage Yield and Quality
• Traditional Ecosystem Services of Forage and Grazing Lands
• Performance and Profitability of Stocker Cattle Grazing KY 31, Jesup MaxQ & Winter Annual Pastures
• Forage Quality of Jesup MaxQ Tall Fescue

Effects Of Recycled Water On Landscape Plants, Casey R. Miranda

Master's Theses

ABSTRACT EFFECTS OF RECYCLED WATER ON LANDSCAPE PLANTS Casey Ray Miranda Recycled water is water that has been previously used, has suffered a loss of quality, and has been properly treated for redistribution (Wu et al. 2001). The use of recycled water as an alternative to fresh water in the landscape can have positive and negative effects. Experimentation on 40 different plant species during a 32 week period (2 phases of 16 weeks), was conducted to analyze the effects of recycled water irrigation on the appearance of landscape plants. Each species of plant was planted into 10 individual number 2 …

Fire: Ecology & Prevention, Justin R. Frey

Social Sciences

No abstract provided.

Poisonous Rangeland Plants In San Luis Obispo County, Sara Litten, Amanda Ou

Animal Science

Poisonous Rangeland Plants in San Luis Obispo County is a comprehensive educational guide to rangeland plants that are toxic to domestic livestock. This guide begins with an exploration of how the biological systems are affected by the poisonous plant toxins. The biochemistry behind these toxins is included in the discussion. Next, reference material for fourteen plants that inhabit San Luis Obispo County is provided. This information includes specific toxins found in poisonous plants, affected animals, symptoms of poisoning, stages of growth, lethal dose, and distribution of the plant in California. This section of the guide is filled with helpful photos …

Good Agricultural Practices For Food Safety Of Fresh Produce, Laurie Hodges

Department of Agronomy and Horticulture: Faculty Publications

What are the guiding principles of food safety for fresh produce?

• Once contaminated, removing or killing pathogens on produce is very difficult.

• Preventing microbial contamination at all steps, from production to distribution, is strongly favored over treatments to eliminate contamination that may have occurred.

• Document the implementation of prevention programs, and educate workers at all levels of the agricultural and packing environments on food safety.

Key Signatures of a Credible Food Safety Program

The following is a condensed checklist of practices and conditions that reduce the potential for fresh produce to be exposed to pathogenic microbes. Review …

Ring-Necked Pheasant Nest Parasitism Of Sharp-Tailed Grouse Nests In Southwest North Dakota, Benjamin A. Geaumont, Kevin K. Sedivec, Christopher S. Schauer

The Prairie Naturalist

Numerous investigators have reported observations of ring-necked pheasant (RNP; Phasianus colchicus) eggs in nests of other gamebirds and waterfowl species (Errington and Hamerstrom 1938, Westemeier et al. 1998, Hagen et al. 2002, Krakauer and Kimball 2009). Previously recorded hosts include wild turkey (Meleagris gallopavo; Schmutz 1988), northern bobwhite (Colinus virginianus; Westemeier et al. 1989), greater prairie-chickens (Tyrnpanuchus cupido; Westemeier et al. 1998), and lesser prairie-chickens (T. pallidicinctus; Hagen et al. 2002). The reported occurrence of nest parasitism during these studies has generally been low with the highest reported occurrences found in …

Participatory Evaluation Of Integrated Pest And Soil Fertility Management Options Using Ordered Categorical Data Analysis, Hugo De Groote, Esther Rutto, George Odhiambo, Fred Kanampiu, Zeyaur Khan, Richard Coe, Bernard Vanlauwe

All Peer-Reviewed Publications

Maize is becoming the major food crop around Lake Victoria. Major constraints to its production are Striga, stem borer, and declining soil fertility. Innovative integrated technologies have been developed: the 'push-pull' system (intercropping with Desmodium and surrounded by Napier grass), soybean and Crotalaria rotations, and imidazolinone-resistant (IR) maize seed. In 12 demonstration trials in four villages in Siaya and Vihiga districts (Kenya) and two villages in Busia (Uganda) in 2003 and 2004, 504 farmers evaluated all cropping systems and a mono-cropped continuous maize, each cropped with IR or local maize, and supplemented or not with fertilizer, totaling 16 treatments. Farmers …

Isoschaftoside, A C-Glycosylflavonoid From Desmodium Uncinatum Root Exudate, Is An Allelochemical Against The Development Of Striga, Antony M. Hooper, Muniru K. Tsanuo, Keith Chamberlain, Kay Tittcomb, Julie Scholes, Ahmed Hassanali, Zeyaur R. Khan, John A. Pickett

All Peer-Reviewed Publications

In East African small-holder farming of maize, the cattle forage legume, Desmodium uncinatum is used as an intercrop due to its allelopathic inhibition of parasitism by Striga hermonthica, an obligate parasitic weed that can devastate the maize crop. Bioassay-guided fractionation of the root extract of D. uncinatum revealed isoschaftoside to be the main compound in the most potent fraction inhibiting growth of germinated S. hermonthica radicles. Bioassays repeated with isoschaftoside isolated from a different plant source, Passiflora incarnata, proved it to be a biologically active component. Analysis of the root exudates produced by hydroponically grown D. uncinatum showed isoschaftoside to …

Vascular Plants Of The High Sierra San Pedro Mártir, Baja California, Mexico: An Annotated Checklist, Robert F. Thorne, Reid V. Moran, Richard A. Minnich

Aliso: A Journal of Systematic and Floristic Botany

The Sierra San Pedro Mártir (SSPM) is the highest mountain range in Baja California, Mexico, the summit of Picacho del Diablo peak attaining 3095 meters. An annotated checklist describes the vascular flora of the SSPM high country, defined here as above 1800 m. It comprises almost 500 species in 251 genera and 78 families. The high country is dominated by coniferous forest species known from montane California and Arizona whose southern ranges terminate in the SSPM. The annotated checklist identifies 453 species in 236 genera as indigenous, of which 23 species and one variety are endemic to the SSPM. Over …

Origin Of The Polycomb Repressive Complex 2 And Gene Silencing By An E(Z) Homolog In The Unicellular Alga Chlamydomonas, Scott Shaver, J. Armando Casas-Mollano, Ronald L. Cerny, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty Publications

Polycomb group proteins play an essential role in the maintenance of cell identity and the regulation of development in both animals and plants. The Polycomb Repressive Complex 2 (PRC2) is involved in the establishment of transcriptionally silent chromatin states, in part through its ability to methylate lysine 27 of histone H3 by the Enhancer of zeste [E(z)] subunit. The absence of PRC2 in unicellular model fungi and its function in the repression of genes vital for the development of higher eukaryotes led to the proposal that this complex may have evolved together with the emergence of multicellularity. However, we report …

Artificial Selection For Determinate Growth Habit In Soybean, Zhixi Tian, Xiaobo Wang, Rian Lee, Yinghui Li, James E. Specht, Randall L. Nelson, Phillip E. Mcclean, Lijuan Qiu, Jianxin Ma

Department of Agronomy and Horticulture: Faculty Publications

Determinacy is an agronomically important trait associated with the domestication in soybean (Glycine max). Most soybean cultivars are classifiable into indeterminate and determinate growth habit, whereas Glycine soja, the wild progenitor of soybean, is indeterminate. Indeterminate (Dt1/Dt1) and determinate (dt1/dt1) genotypes, when mated, produce progeny that segregate in a monogenic pattern. Here, we show evidence that Dt1 is a homolog (designated as GmTfl1) of Arabidopsis terminal flower 1 (TFL1), a regulatory gene encoding a signaling protein of shoot meristems. The transition from indeterminate to determinate phenotypes in soybean is associated …

Characteristics Of Nectar Production And Standing Crop In Campsis Radicans (Bignoniaceae)., Andrea Alexis Edge

Electronic Theses and Dissertations

We examined several aspects of nectar production in Campsis radicans to better understand how standing crop is affected and how production affects pollinator visitation. In all experiments, nectar and concentration of flowers were measured and total sugar was calculated. Flowers do not produce additional nectar unless nectar is removed, and it is not resorbed. Volume of standing crop and total sugar fluctuates throughout the day, whereas concentration remains constant. Age and time of day significantly affect regeneration of nectar and sugar. The number of removals did not significantly affect the amount of nectar or sugar regenerated; however, concentration declined significantly …

Nebline, May 2010

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Groundwater, a Vital Nebraska Resource

Farm Views

Horticulture

Food & Fitness

Home & Family Living

Environmental Focus

Urban Agriculture

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Establishment And Management Practices Of Two New Warm-Season Turfgrasses In The Southern Transition Zone, John Stiglbauer

All Theses

Seashore paspalum (Paspalum vaginatum O. Swartz) is a turfgrass that has become increasingly popular in southern coastal regions of the US because of its ability to tolerate salt stress. Another reason that this turfgrass is increasing in popularity is its aesthetically appealing striping ability. Seashore paspalum could also be beneficial in areas where potable water cannot be used for irrigation. Information on this turfgrass is limited in literature, particularly on the topics of establishment, scalping, and growing this turfgrass on inland sites in the transition zone. A field experiment was conducted in Clemson, SC from May 2007 and 2008 to …

Forage News [2010-05], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Grazing School
• Barren County to Host KFGC Field Day
• National and International Meeting Coming to Kentucky
• Forage Quality, Ethanol Production Studied
• Teff Grass
• Pennsylvania Forage and Grassland Council Celebrating 50th Birthday
• Spontaneous Combustion in Hay
• Change in Yield and Quality of Alfalfa
• Hay Inoculants and Preservatives

Dna Viruses: The Really Big Ones (Giruses), James L. Van Etten, Leslie C. Lane, David Dunigan

Department of Plant Pathology: Faculty Publications

Viruses with genomes greater than 300 kb and up to 1200 kb are being discovered with increasing frequency. These large viruses (often called giruses) can encode up to 900 proteins and also many tRNAs. Consequently, these viruses have more protein-encoding genes than many bacteria, and the concept of small particle/small genome that once defined viruses is no longer valid. Giruses infect bacteria and animals although most of the recently discovered ones infect protists. Thus, genome gigantism is not restricted to a specific host or phylogenetic clade. To date, most of the giruses are associated with aqueous environments. Many of these …

Effect Of Grazing System On Livestock Performance, Botanical Composition, And Standing Crop In The Nebraska Sandhills, Mitchell B. Stephenson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A 10-year study compared a 4-pasture deferred-rotation (DR) grazing system with an 8-pasture short duration grazing (SDG) system at the Barta Brothers Ranch near Rose, NE to determine differences in livestock performance, botanical composition, and standing crop. Pastures were grazed by 50 to100 cow-calf pairs with both single (DR system) and multiple (SDG system) grazing periods from 15 May to 15 October. Livestock performance data were collected from spayed heifers substituted into each grazing system during the last 3 years of the study. Botanical composition was collected on 275 frequency of occurrence transects in 1998, 2003, and 2008. Standing crop …

Comparative Performance Of Fluorometry And High Performance Liquid Chromatography In The Detection Of Aflatoxin M1 In Two Commercial Cheeses, Gustavo Peña

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Aflatoxin M1 (AFM₁) is frequently found in milk and dairy products. It is a metabolite formed in cows from aflatoxin B1 (AFB₁), contained in animal feeds. In cheese production AFM₁ distributes between curds and whey. In this study, cows were fed 64 µg/AFB₁/d for the high treatment, and 5 µg/AFB₁/d for the low treatment, to obtain milk contaminated with AFM1 over the 0.5 µg/L and under 0.05 µg/L restrictions, respectively. Cheese was manufactured with milk contaminated with AFM₁ at 0.8 and 0.03 ìg/kg by the higher and lower …