Digital Commons Network^™

Productivity Of Well-Watered Panicum Virgatum Does Not Increase With Co2 Enrichment, Philip A. Fay, H. Wayne Polley, Virginia L. Jin, Michael J. Aspinwall

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aims Rising atmospheric CO₂ has been shown to increase aboveground net primary productivity (ANPP) in water-limited perennial grasslands, in part by reducing stomatal conductance and transpiration, thereby reducing depletion of soil moisture. However, the benefits of CO₂ enrichment for ANPP will vary with soil type and may be reduced if water limitation is low. Little is known about CO₂ effects on ANPP of Panicum virgatum, a perennial C₄ tallgrass and potential bioenergy crop. We hypothesized that if water limitation is minimized, (i) CO₂ enrichmentwould not increase P. virgatum ANPP because photosynthetic rates of this C …

Growing South Dakota (Fall 2012), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Kudos To South Dakota Farmers: SDSU Study Credits Farmers For Conserving Soil, Sequestering More Carbon
[Page] 4 Fall College News
[Page] 6 Investing In Research: Additional Appropriations Being Sought For SDSU’s Ag Station Research
[Page] 8 Pertinent Publications: Plant Science Department Produces Several New Guide Books
[Page] 9 Special Pull-Out Section: Snapshot from SDSU’s College of Agriculture & Biological Sciences: Highlighting Academic Programs, Ag Experiment Station and SDSU Extension
[Page] 13 Plans Proceed: Efforts For Cow/Calf Training And Research Center Continue
[Page] 14 Outreach Through Horses: Initiative Teaches Equine Care On South Dakota Reservations
[Page] 16 Here And …

Functions Of The Arabidopsis Kinesin Superfamily Of Microtubule-Based Motor Proteins, Chuanmei Zhu, Ram Dixit

Biology Faculty Research

Plants possess a large number of microtubule-based kinesin motor proteins. While the kinesin-2, 3, 9, and 11 families are absent from land plants, the kinesin-7 and 14 families are greatly expanded. In addition, some kinesins are specifically present only in land plants. The distinctive inventory of plant kinesins suggests that kinesins have evolved to perform specialized functions in plants. Plants assemble unique microtubule arrays during their cell cycle, including the interphase cortical microtubule array, preprophase band, anastral spindle and phragmoplast. In this review, we explore the functions of plant kinesins from a microtubule array viewpoint, focusing mainly on Arabidopsis kinesins. …

Evolution On The Hawaiian Hotspot: Biogeography And Divergence Time Estimation Of Kadua (Rubiaceae), Kenneth L. Parker

Biological Sciences Theses & Dissertations

Recent studies on the colonization time of Hawaiian plant and animal lineages have suggested certain lineages arrived to the archipelago before the formation of the extant islands. Kadua, a recently resurrected genus, is mostly endemic to the Hawaiian Archipelago, with many member taxa autochthonous to individual islands. This work investigates the colonization time of Kadua to the Hawaiian Islands. Sequence analyses of the quickly evolving nuclear regions ITS, ETS and 5s-NTS regions for 20 Hawaiian and 7 French Polynesian species of Kadua were used to create dated phylogeny calibrated with island ages. The phylogeny suggests a single colonization of Hawaii …

Naturalization Of Sequoiadendron Giganteum (Cupressaceae) In Montane Southern California, Rudolf Schmid, Mena Schmid

Aliso: A Journal of Systematic and Floristic Botany

After the August 1974 fire in the upper Hall Canyon area on the southwestern flank of Black Mountain in the northwestern San Jacinto Mountains, Riverside County, California, the United States Forest Service revegetated the burn in the mixed-conifer forest with the Sierra Nevada endemic Sequoiadendron giganteum (Cupressaceae). On 1 May 2009 a GPS census starting at the head of Hall Canyon revealed both in the canyon and upslope beyond it at least 157 individuals in the vicinity of the Black Mountain Trail, plus an outlier 450 m distant near the summit. This species alien to southern California is regenerating prolifically …

Influence Of Microsite Disturbance On The Establishment Of Two Congeneric Invasive Thistles, Emily S.J. Rauschert, Katriona Shea

Biological, Geological, and Environmental Faculty Publications

The successful establishment of invasive species has been shown to depend on aspects of the invaded community, such as gap characteristics. Biotic resistance may be particularly critical for stopping invaders at early life history stages, but new species can often invade following disturbances, which may create microsites with very different characteristics than are usually present. We examine the response of two invasive thistle species, Carduus nutans L. and C. acanthoides L., to three different microsite characteristics: disturbance type, size, and water availability. The two species initially responded differently to the type of disturbance: C. acanthoides had higher emergence and survival …

Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston

Plant and Soil Sciences Faculty Patents

The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.

Asareca-Intsormil: Regional Sorghum Research And Development Workshop, September 2–6, 2012, Executive Hotel, Adama, Ethiopia, Kimberly Christiansen

INTSORMIL Scientific Publications

Priorities in Sorghum Research and Development Cross-Cutting Elements

All priorities should be cognizant and address:

Gender responsiveness

User friendliness in sharing knowledge and information

Sustainable growth

Capacity building

Collaboration with public and private partners especially NARS, and regional and international organizations

Production

Integrated soil and water management in response to climate change

Integrated pest management

Cropping systems

Mechanization to alleviate drudgery

Breeding/Genetics

Breeding high yielding sorghum cultivars with enhanced ecological adaptation (drought tolerance and Striga resistance and diseases and pests) and superior grain quality

Development, release and distribution of enhanced sorghum germplasm materials with desired end-use quality attributes (malt, biofuel, …

Local Conditions, Not Regional Gradients, Drive Demographic Variation Of Giant Ragweed (Ambrosia Trifida) And Common Sunflower (Helianthus Annuus) Across Northern U.S. Maize Belt, Sam E. Wortman, Adam S. Davis, Brian J. Schutte, John L. Lindquist, John Cardina, Joel Felix, Christy L. Sparague, Anita Dille, Analiza H.M. Ramirez, Graig Reicks, Sharon A. Clay

Agronomy, Horticulture and Plant Science Faculty Publications

Knowledge of environmental factors influencing demography of weed species will improve understanding of current and future weed invasions. The objective of this study was to quantify regional-scale variation in vital rates of giant ragweed and common sunflower. To accomplish this objective, a common field experiment was conducted across seven sites between 2006 and 2008 throughout the north central U.S. maize belt. Demographic parameters of both weed species were measured in intra- and interspecific competitive environments, and environmental data were collected within site-years. Site was the strongest predictor of belowground vital rates (summer and winter seed survival and seedling recruitment), indicating …

Forage News [2012-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Forage Field Day
• Kentucky Grazing School
• Beef Bash 2012
• National Hay Association to Feature Kentucky Speakers
• 13th Kentucky Grazing Conference
• Partridge Pea or Chamaecrista fasciculate (Michx.) Greene
• Forage Brassica Crops in Grazing Systems
• Darkling/Mealworm Beetles can cause "Blister Beetle Scare"

Functional Analysis Of Three Arabidopsis Argonautes Using Slicer-Defective Mutants, Alberto Carbonell, Noah Fahlgren, Hernan Garcia-Ruiz, Kerrigan B. Gilbert, Taiowa A. Montgomery, Tammy Nguyen, Josh T. Cuperus, James C. Carrington

Department of Plant Pathology: Faculty Publications

In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicerindependent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were used to examine several AGO functions, including small RNA binding, interaction with target RNA, slicing or destabilization of target RNA, secondary small interfering RNA formation, and antiviral activity. Complementation analyses in ago mutant plants revealed that the catalytic residues of AGO1, AGO2, and AGO7 are required to restore the defects …

Rosette Iron Deficiency Transcript And Microrna Profiling Reveals Links Between Copper And Iron Homeostasis In Arabidopsis Thaliana, Brian M. Waters, Samuel A. Mcinturf, Ricardo J. Stein

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) is an essential plant micronutrient, and its deficiency limits plant growth and development on alkaline soils. Under Fe deficiency, plant responses include up-regulation of genes involved in Fe uptake from the soil. However, little is known about shoot responses to Fe deficiency. Using microarrays to probe gene expression in Kas-1 and Tsu-1 ecotypes of Arabidopsis thaliana, and comparison with existing Col-0 data, revealed conserved rosette gene expression responses to Fe deficiency. Fe-regulated genes included known metal homeostasis-related genes, and a number of genes of unknown function. Several genes responded to Fe deficiency in both roots and rosettes. …

Lawn Fertilizers For Cool Season Turf, Taun Beddes, Kelly Kopp

All Current Publications

Lawns provide a durable and functional surface for many outdoor activities while adding beauty to the landscape. For these reasons, a healthy, green lawn is the goal of many homeowners and landscape managers. Keeping a lawn healthy and green may require many management practices such as mowing, irrigation and aerification. Often, these practices will include fertilizing with supplemental nutrients at various times during the growing season. This promotes optimum turf color, improves re-growth, and increases tolerance to wear, drought, insects and disease. The broad number of fertilizer options available from retailers can make choosing a fertilizer difficult. However, choosing an …

Plant Calcium Content: Ready To Remodel, Jian Yang, Tracy Punshon, Mary Lou Guerinot, Kendal D. Hirschi

Dartmouth Scholarship

By identifying the relationship between calcium location in the plant cell and nutrient bioavailability, the plant characteristics leading to maximal calcium absorption by humans can be identified. Knowledge of plant cellular and molecular targets controlling calcium location in plants is emerging. These insights should allow for better strategies for increasing the nutritional content of foods. In particular, the use of preparation-free elemental imaging technologies such as synchrotron X-ray fluorescence (SXRF) microscopy in plant biology may allow researchers to understand the relationship between subcellular location and nutrient bioavailability. These approaches may lead to better strategies for altering the location of calcium …

Nebline, August 2012

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 9 Reasons to Shop at Farmers Markets

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

High School Horticulture Curriculum, Margaret Maratsos

Horticulture and Crop Science

Horticulture is just one of the many topics covered in a high school agriculture curriculum, and yet, there are very few lesson plans or resources available to teachers for these classes specifically. The objectives of this project were to compile a set of lesson plans, lab plans, and tests that would emphasize interactive and investigative learning. The lesson plans were written in such a way that they reflected a certain set of standards, set down by the state of California, and were meant to serve as a bare outline of topics that would be discussed within a week’s worth of …

Effects Of Meloidogyne Incognita, Soil Physical Parameters, And Thielaviopsis Basicola On Cotton Root Architecture And Plant Growth, Jianbing Ma

Graduate Theses and Dissertations

The root-knot nematode, Meloidogyne incognita, and the seedling pathogen, Thielaviopsis basicola, commonly co-exist in Arkansas cotton fields and may interact resulting in increased losses. The primary objective of this research was to evaluate the effects of soil physical parameters on these soilborne pathogens and cotton growth in controlled environmental, field, and microplot studies. Controlled environmental experiments used two soil bulk densities and four pathogen treatments: non-infested soil, soil infested with M. incognita or T. basicola and soil infested with both pathogens. The results indicated bulk density generally did not affect seedling growth or disease since soils had low penetration resistance …

Forage News [2012-08], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Field Day - Hart County - September 6
• Drought and Forages
• Choose the Best Variety for Fall Plantings
• Plan to Attend the AFGC Meeting in Covington, KY - Jan. 7-9, 2013
• High Nitrates Especially in Drought Stressed Corn
• Biofuels will Continue to Drive Feed Prices
• Mother Nature Throws a Wrench into 2012

The Identification Of Quantitative Trait Loci For Sucrose And Stachyose Content In Soybean Seed, Ailan Zeng

Graduate Theses and Dissertations

Sucrose is a desirable sugar in soybean seed that affects the quality and taste of soyfoods, while stachyose is a non-digestible sugar that induces flatulence in non-ruminant animals. Therefore, soybean cultivars with high sucrose and/or low stachyose would be valuable for soyfood and meal markets. The objectives of this study were to identify quantitative trait loci (QTL) or genes associated with seed sucrose and stachyose content using simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers. A low sucrose line, MFS-553, was crossed with a high sucrose plant introduction, PI 243545, to develop the sucrose QTL mapping population. A …

High-Throughput Transcriptome Sequencing For Snp And Gene Discovery In A Moth, Nicholas J. Miller, Jing Sun, Thomas W. Sappington

Department of Entomology: Faculty Publications

The western bean cutworm, Striacosta albicosta (Smith) (Leptidoptera: Noctuidae) is a pest of corn (Zea mays L.) and dry beans that underwent a dramatic range expansion in North America during the first decade of the 21st century. Research into the population genetics of this species has been hindered by a lack of genetic markers. The transcriptome of adult male S. albicosta was partially sequenced using Illumina sequencing-by-synthesis. Assembly of the sequence reads yielded 16,847 transcript sequences, of which 6,631 could be assigned a putative function. A search for single nucleotide polymorphisms (SNPs) identified 2,487 candidate SNPs distributed among 1,265 …

Is Local Food In Your Future?: An Analysis Of The Viability Of The Local Food Movement, Jennifer A. Sliney

Pell Scholars and Senior Theses

The Local Food Movement has arisen in recent decades as a proposed solution to problems in the current food distribution system. Proponents of the movement look to solve problems such as unsustainable farming practices, greenhouse gas emissions, and unhealthy communities. Skeptics raise questions as to whether local food is truly capable of accomplishing any of those. The author proposes that as a solution, aspects of the Local Food Movement such as urban agriculture and attempts at community development should be taken more seriously in order to create a better future for the land and people alike.

Genetic Characterization Of Fine-Leaved Festuca Valesiaca Germplasm And Evaluation Of Their Relationship To The Festuca Ovina Complex, Yingmei Ma

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fine-leaved Festuca valesiaca has stress tolerance. However, its agronomic performance in the western United States and its genetic relationship to species of the Festuca ovina complex has not been investigated. Also, natural hybridization makes them difficult to identify. Given the species' potentials, our project was designed to identify Festuca valesiaca accessions that possess high biomass and seed yield and to examine their relatedness with the Festuca ovina complex.

The Festuca valesiaca accessions examined produced many small seeds. Seed production was correlated with the total biomass, plant height, and plant vigor. The Festuca valesiaca accessions examined were shorter than the control …

The Impact Of Selected Forage Legumes On Cattle Performance, Forage Production, And Soil Quality, And Evaluation Of Legume Persistence Under Grazing, Bradley Edward Briggs

Graduate Theses and Dissertations

Interest in substituting legumes for N fertilizer in beef cattle grazing systems has recently increased with rising fertilizer prices. Legumes are well known for their ability to fix atmospheric N and decrease dependence on input of N fertilizer. However, there are still difficulties associated with legume utilization including establishment and persistence. Two experiments were conducted to evaluate legume performance under herbivory. The objective of Experiment 1 was to compare forage production and beef cattle gains from annual ryegrass [Lolium multiflorum (L.)] and bermudagrass [Cynodon dactylon (L.) Pers.] pastures fertilized with N or overseeded with legumes. Gelbvieh × Angus …

Flight Period And Species Composition Of Sirex (Hymenoptera: Siricidae) And Associated Deladenus (Nematoda: Neotylenchidae) Within Arkansas Pine Forests, Danielle Keeler

Graduate Theses and Dissertations

The European woodwasp, Sirex noctilio F. (Hymenoptera: Siricidae), which is a known destructive pest of pine in the southern hemisphere was recently discovered in the eastern United States. Before we can understand how S. noctilio may affect pine forests throughout the United States, we need a better understanding of native Sirex and the role they play in the ecosystem. The objectives of this research were to 1) determine species composition and flight period for native Sirex; 2) confirm presence of parasitic nematodes, Deladenus (Nematoda: Neotylenchidae), within adult Sirex; 3) investigate Deladenus parasitism rates; 4) verify the number of Deladenus species …

Regulation Of Mirna Abundance By Rna Binding Protein Tough In Arabidopsis, Guodong Ren, Meng Xie, Yongchao Dou, Shuxin Zhang, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MicroRNAs (miRNAs) are regulators of gene expression in plants and animals. The biogenesis of miRNAs is precisely controlled to secure normal development of organisms. Here we report that TOUGH (TGH) is a component of the DCL1–HYL1–SERRATE complex that processes primary transcripts of miRNAs [i.e., primary miRNAs (pri-miRNAs)] into miRNAs in Arabidopsis. Lack of TGH impairs multiple DCL activities in vitro and reduces the accumulation of miRNAs and siRNAs in vivo. TGH is an RNA-binding protein, binds pri-miRNAs and precursor miRNAs in vivo, and contributes to pri-miRNA–HYL1 interaction. These results indicate that TGH might regulate abundance of miRNAs through promoting DCL1 …

Discovery Of Novel Dsrna Viral Sequences By In Silico Cloning And Implications For Viral Diversity, Host Range And Evolution, Huiquan Liu, Yanping Fu, Jiatao Xie, Jiasen Cheng, Said A. Ghabrial, Guoqing Li, Xianhong Yi, Daohong Jiang

Plant Pathology Faculty Publications

Genome sequence of viruses can contribute greatly to the study of viral evolution, diversity and the interaction between viruses and hosts. Traditional molecular cloning methods for obtaining RNA viral genomes are time-consuming and often difficult because many viruses occur in extremely low titers. DsRNA viruses in the families, Partitiviridae, Totiviridae, Endornaviridae, Chrysoviridae, and other related unclassified dsRNA viruses are generally associated with symptomless or persistent infections of their hosts. These characteristics indicate that samples or materials derived from eukaryotic organisms used to construct cDNA libraries and EST sequencing might carry these viruses, which were not easily detected by the researchers. …

Utility Of Phylloplanins As Antibiotics, Selective Fungicides And For Enhancing Microbial Resistance In Plants, George Wagner, Ryan Sheperd

Plant and Soil Sciences Faculty Patents

The present invention relates to plant proteins produced by a plant's epidermal layer that contribute to the innate pest/disease resistance of the plant (“phylloplanins”), compositions comprising the phylloplanins and methods of using them. In particular the methods relate to inhibiting or preventing microbial, e.g., fungal or bacterial, growth on a subject, organism or surface by administering a phylloplanin. The invention also relates to transformed host cells that produce phylloplanins, and to transgenic plants producing phylloplanins conferring increased resistance to microbial infections/growth.

Genetic Structure And Mating System Of Wild Cowpea Populations In West Africa, Eric B. Kouam, Remy S. Pasquet, Pascal Campagne, Jean Baptiste Tignegre, Kevin Thoen, Remi Gaudin, Jeremy T. Ouedraogo, Abdulai B. Salifu, Geoffrey M. Muluvi, Paul Gepts

All Peer-Reviewed Publications

Background: Cowpea is a highly inbred crop. It is part of a crop-weed complex, whose origin and dynamics is unknown, which is distributed across the African continent. This study examined outcrossing rates and genetic structures in 35 wild cowpea (Vigna unguiculata ssp. unguiculata var. spontanea) populations from West Africa, using 21 isozyme loci, 9 of them showing polymorphism.Results: Outcrossing rates ranged from 1% to 9.5% (mean 3.4%), which classifies the wild cowpea breeding system as primarily selfing, though rare outcrossing events were detected in each population studied. Furthermore, the analyses of both the genetic structure of populations and the relationships …

Feed The Future Releases Two New Sorghum Varieties In Nicaragua, Kimberly Christiansen

INTSORMIL Impacts and Bulletins

de Tecnologia Agropecuaria (INTA), the Collaborative Research Support Program for Sorghum, Millet and Other Grains (INTSORMIL) has released two new varieties of sorghum in Nicaragua that will be used for forage (plant material eaten by grazing livestock).

These new varieties exhibit the “brown midrib” trait (bmr), which has been used for many years by sorghum producers in the United States. The bmr trait increases the digestibility of sorghum by reducing the amount of lignin, a chemical compound found in the cell walls of plants. The more digestible sorghum is for the livestock that consume it, the higher the quality of …

Invasional Interference Due To Similar Inter- And Intraspecific Competition Between Invaders May Affect Management, Emily S.J. Rauschert, Katriona Shea

Biological, Geological, and Environmental Faculty Publications

As the number of biological invasions increases, the potential for invader– invader interactions also rises. The effect of multiple invaders can be superadditive (invasional meltdown), additive, or subadditive (invasional interference); which of these situations occurs has critical implications for prioritization of management efforts. Carduus nutans and C. acanthoides, two congeneric invasive weeds, have a striking, segregated distribution in central Pennsylvania, USA. Possible hypotheses for this pattern include invasion history and chance, direct competition, or negative interactions mediated by other species, such as shared pollinators. To explore the role of resource competition in generating this pattern, we conducted three related experiments …