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Mapping Quantitative Trait Loci For Resistance To Goss’S Bacterial Wilt And Leaf Blight In North American Maize By Joint Linkage Analysis, Amritpal Singh, Aaron P. Andersen, Tamra A. Jackson-Ziems, Aaron J. Lorenz

Department of Plant Pathology: Faculty Publications

Goss’s wilt and leaf blight is a bacterial disease of maize (Zea mays L.) caused by the Gram-positive bacterium Clavibacter michiganensis subsp. nebraskensis. Goss’s wilt has reemerged as an important disease in the western United States and is spreading to other areas. Although the reasons for this reemergence are not completely known, it is important to understand the genetic basis of resistance to Goss’s wilt. The objective of this study was to map the quantitative trait loci (QTL) underlying resistance to Goss’s wilt. To achieve this objective, joint linkage and linkage mapping in 3 of the 25 nested …

Corn Yield Loss Estimates Due To Diseases In The United States And Ontario, Canada From 2012 To 2015, Daren S. Mueller, Kiersten A. Wise, Adam J. Sisson, Tom W. Allen, Gary C. Bergstrom, D. Bruce Bosley, Carl A. Bradley, Kirk D. Broders, Emmanuel Byamukama, Martin I. Chilvers, Alyssa Collins, Travis R. Faske, Andrew J. Friskop, Ron W. Heiniger, Clayton A. Hollier, David C. Hooker, Tom Isakeit, Tamra A. Jackson-Ziems, Douglas J. Jardine, Heather M. Kelly, Kasia Kinzer, Steve R. Koenning, Dean K. Malvick, Marcia Mcmullen, Ron F. Meyer, Pierce A. Paul, Alison E. Robertson, Gregory W. Roth, Damon L. Smith, Connie A. Tande, Albert U. Tenuta, Paul Vincelli, Fred Warner

Department of Plant Pathology: Faculty Publications

Annual decreases in corn yield caused by diseases were estimated by surveying members of the Corn Disease Working Group in 22 corn-producing states in the United States and in Ontario, Canada, from 2012 through 2015. Estimated loss from each disease varied greatly by state and year. In general, foliar diseases such as northern corn leaf blight, gray leaf spot, and Goss’s wilt commonly caused the largest estimated yield loss in the northern United States and Ontario during nondrought years. Fusarium stalk rot and plant-parasitic nematodes caused the most estimated loss in the southern-most United States. The estimated mean economic loss …

Sheep Updates 2016, Mick Keogh, David Cornish, Michael Chilvers, Gerry Hinkley, Danielle England, Rosemary Bartle, Brad Wooldridge, Norm Santich, John Young, Ashley Herbert, Jamie Heinrich, Graeme Howie, Bruce Mullan

Sheep Updates

This session covers eleven papers from different authors:

1. Forward, Dr Bruce Mullan, Director Sheep Industry Development, Department of Agriculture and Food Western Australia

2. The Australian sheep industry in 2025, Mick Keogh, Australian Farm Institute

3. Decision making in a risky environment, David Cornish, Cornish Consulting

4. Business Transitioning, Michael Chilvers, Nile Tasmania

5. Advisory Boards in WA Agriculture - making life 'easier' for everyone, Gerry Hinkley, Producer, Tincurrin and Danielle England, Aginnovate

6. Principles of Successful Family Business Succession Strategies, Rosemary Bartle, Succession Planning Facilitator, Rabobank

7. Diversifying the Feedbase...is the …

Bacterial Leaf Streak Of Corn Confirmed In Nebraska, Other Corn Belt States August 26, 2016, Tamra Jackson-Ziems, Kevin A. Korus, Tony Adesemoye, Julie Van Meter

Department of Plant Pathology: Faculty Publications

Bacterial leaf streak disease of corn, caused by Xanthomonas vasicola pv. vasculorum, has now been confirmed in Nebraska, as well as in Colorado, Illinois, Iowa, and Kansas. The disease had not been previously identified in the U.S., but had been reported on corn in South Africa. Surveys are currently underway across the Corn Belt to identify the disease distribution. Initial observations and survey results suggest that it may be widely distributed throughout the Corn Belt. Unusual symptoms were first reported on corn samples received by the University of Nebraska Plant and Pest Diagnostic Clinic. Over the last two years, the …

The Floral Charms Of Saint John’S: A Survey Of Botanical Communities, Stephen G. Saupe, Paul Melchior

Headwaters

Saint John’s is a “landscape paradise” in central Minnesota. Although no complete checklist of vegetation exists for the campus, various surveys reveal a diverse flora. This floral wealth is the result of several terrestrial biomes converging on campus — prairie, savanna, deciduous forest, and coniferous forest — as well as diverse aquatic habitats including both lakes and wetlands. A 56-acre restored prairie at the entrance of Saint John’s is dominated by big bluestem and little bluestem. A savanna has been restored to the north and east of the Gemini Lakes and is characterized by bur oaks scattered among prairie grasses …

In Vitro Motility Of Actin Filaments Powered By Plant Myosins Xi, Cesar A. Quintana-Cataño, Christopher J. Staiger, Weiwei Zhang

The Summer Undergraduate Research Fellowship (SURF) Symposium

The actomyosin network is thought to support fundamental processes of plant development and cell expansion such as polarized elongation of root hairs and the diffuse growth of epidermal and mesophyll cells. Inhibition of myosins via pharmacological treatments represents one of the key approaches for understanding of their roles in different cellular processes. However, the use of the standard plant myosin inhibitor, 2,3-butanedionemonoxime (BDM), is questioned as it requires a high concentration and may not be as specific as desired. By testing drugs that inhibit animal and yeast myosins V, the Staiger laboratory previously found pentabromopseudilin (PBP) as a potential inhibitor …

Bulletin No. 42: The Mamacoke Conservation Area, Glenn Dreyer, Robert Askins, Scott Peterson

Bulletins

No abstract provided.

Agro-Climatic Change, Crop Production And Mitigation Strategies-Case Studies In Arkansas, Usa And Kenya, John Westley Magugu

Graduate Theses and Dissertations

Although climate change impacts vary geographically and temporally, studies at local levels are not readily available for stakeholders to better understand how their local communities would be affected and what remedial measures could be more effective in their local contexts. This dissertation has examined climate change and its impacts in two different local contexts: eastern Arkansas in the USA and Nyando in Kenya. The first part of this dissertation develops agro-meteorological indicators and examines the relationship between agro-meteorological indicators and crop yields in eastern Arkansas between 1960 and 2014. Results reveal that temperature based indicators were more strongly correlated to …

Forage News [2016-08], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Eden Shale Farm to Host KFGC Field Day Sept. 13th
• Forage News Quote of the Month: "Forages, particularly the Grasses, are the most important plants on the face of the earth"
• Sugarcane Aphid Found in Kentucky
• UK Breeder Develops New Tall Fescue Variety
• Featured Publication: Stockpiling for Fall and Winter Pasture (AGR-162)
• Planting Brassicas and Cereals for Fall Forage
• Choose Varieties Wisely

Validation Of Reference Genes For Robust Qrt-Pcr Gene Expression Analysis In The Rice Blast Fungus Magnaporthe Oryzae, Sarena Che Omar, Michael A. Bentley, Giulia Morieri, Gail M. Preston, Sarah J. Gurr, Richard Wilson (Editor)

Department of Plant Pathology: Faculty Publications

The rice blast fungus causes significant annual harvest losses. It also serves as a genetically- tractable model to study fungal ingress. Whilst pathogenicity determinants have been unmasked and changes in global gene expression described, we know little about Magnaporthe oryzae cell wall remodelling. Our interests, in wall remodelling genes expressed during infection, vegetative growth and under exogenous wall stress, demand robust choice of reference genes for quantitative Real Time-PCR (qRT-PCR) data normalisation. We describe the expression stability of nine candidate reference genes profiled by qRT-PCR with cDNAs derived during asexual germling development, from sexual stage perithecia and from vegetative mycelium …

Mapping And Analyzing Energy Use And Efficiency In A Modified Hydroponic Shipping Container, Rachel E. Sparks

Open Access Theses

In urban centers today, vertical farming is becoming a popular alternative to conventional agriculture in an effort to increase local food production and improve urban food security by growing crops using hydroponic methods in controlled environment spaces. More specifically, one vertical method involves growing crops inside refurbished shipping containers, or a “farm-in-a-box” concept, which offers a flexible, mobile, and scalable means of year-round food production in a variety of climates. Despite benefits of producing food locally, some of the concerns associated with these vertical farming systems include high energy consumption from climate control and electric lighting systems as well as …

Understanding Plant Response To Stress Using Gene Model Quality Evaluation And Transcriptome Analysis, Karthik Ramaswamy Padmanabhan

Open Access Dissertations

The overall aim of the project was to understand how plants reacted to environmental stress and evolved to overcome it. The land plants that we see today evolved from a green algal ancestor around 510 million years ago. Plants had to make significant changes to their cellular, morphological, regulatory and physiological processes during their adaptation to the terrestrial environment from an aquatic environment. The first part of the project was to find out how these changes were reflected on the protein makeup of the early land plants. The gene model sequence data of two early land plants, Physcomitrella patens (moss) …

Nebline, August 2016

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Clover College 20th Anniversary — Grows From 8 to 58 Workshops

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

Extension Calendar

and other extension news and events

Special pullout section: Lancaster County Super Fair 2016 Schedule & Map

Genetic Basis Of Biosynthesis And Cytotoxic Activity Of Medicago Truncatula Triterpene Saponins, Brynn Kathleen Lawrence

Graduate Theses and Dissertations

Saponins are a large family of specialized metabolites produced in many plants. They can have negative effects on a number of plant pests and are thought to play a role in plant defense. With current and possible future uses in industry and agriculture, saponins have also been shown to be hypocholesterolemic, hypoglycemic, immunostimulatory, antioxidative, anti-inflammatory, and cytotoxic. In spite of their usefulness, our understanding of the genetic basis for saponin biosynthesis is still incomplete. We generated recombinant populations with parents from genetically distinct accessions of Medicago truncatula, with either high or low accumulation and varying profiles of saponins. Primers for …

Chromosome-End Knockoff Strategy To Reshape Alkaloid Profiles Of A Fungal Endophyte, Simona Florea, Timothy D. Phillips, Daniel G. Panaccione, Mark L. Farman, Christopher L. Schardl

Plant Pathology Faculty Publications

Molecular genetic techniques to precisely eliminate genes in asexual filamentous fungi require the introduction of a marker gene into the target genome. We developed a novel strategy to eliminate genes or gene clusters located in subterminal regions of chromosomes, and then eliminate the marker gene and vector backbone used in the transformation procedure. Because many toxin gene clusters are subterminal, this method is particularly suited to generating nontoxic fungal strains. We tested this technique on Epichloë coenophiala, a seed-transmissible symbiotic fungus (endophyte) of the important forage grass, tall fescue (Lolium arundinaceum). The endophyte is necessary for maximal …

Identification Of Diverse Mycoviruses Through Metatranscriptomics Characterization Of The Viromes Of Five Major Fungal Plant Pathogens, Shin-Yi Lee Marzano, Berlin D. Nelson, Olutoyosi Ajayi-Oyetunde, Carl A. Bradley, Teresa J. Hughes, Glen L. Hartman, Darin M. Eastburn, Leslie L. Domier

Plant Pathology Faculty Publications

Mycoviruses can have a marked effect on natural fungal communities and influence plant health and productivity. However, a comprehensive picture of mycoviral diversity is still lacking. To characterize the viromes of five widely dispersed plant-pathogenic fungi, Colletotrichum truncatum, Macrophomina phaseolina, Diaporthe longicolla, Rhizoctonia solani, and Sclerotinia sclerotiorum, a high-throughput sequencing-based metatranscriptomic approach was used to detect viral sequences. Total RNA and double-stranded RNA (dsRNA) from mycelia and RNA from samples enriched for virus particles were sequenced. Sequence data were assembled de novo, and contigs with predicted amino acid sequence similarities to viruses in the …

A Review On The Invasion Ecology Of Amur Honeysuckle (Lonicera Maackii, Caprifoliaceae), A Case Study Of Ecological Impacts At Multiple Scales, Rachel E. Mcneish, Ryan W. Mcewan

Biology Faculty Publications

Invasive species are of global importance because of their impacts on ecological communities, habitat structure, native community dynamics, and ecosystem processes and function. Scientists and conservation managers are increasingly focusing on the biological impacts of invasive species and on devising management practices that emphasize the health of ecosystems based on measured biological processes. Amur honeysuckle (Lonicera maackii (Rupr.) Herder) is a highly successful invasive shrub in forests of eastern North America. The scientific literature surrounding this species has grown in the past several decades as researchers have investigated L. maackii impacts across multiple ecological scales. In this review we …

Amplified Fragment Length Polymorphism (Aflp) Analysis Of Taeniatherum Caput-Medusae, An Invader Of Western U.S. Rangelands, Elizabeth Catherine Kent

Boise State University Theses and Dissertations

Biological invasions are one of the main drivers of global change, and thus one of the main factors contributing to a loss of biodiversity world-wide. Introduced species can destroy habitat through predation, grazing, and competition for resources; spread disease; alter disturbance regimes; and disrupt ecosystem services. Taeniatherum caput-medusae subsp. asperum (medusahead) is a winter-annual grass native to Eurasia and invasive in the westernUnited States. Medusahead dominates one million hectares of its invasive range and detrimentally affects the areas it inhabits through degradation of foraging value for livestock, increasing fire frequencies, and decreasing biodiversity. Previously, allozyme analyses have suggested this …

Morphological And Molecular Identification And Characterization Of Dry Bean Fungal Root Rot Pathogens In Zambia, Chikoti Mukuma

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Dry bean is among the most important food legume crops for direct human consumption in Latin America and Africa. Recently, root and crown rot (RCR) has emerged as an important production constraint. Root and crown rot often involve fungal complexes. Thus, the straw, detached leaf, cup and stem tests were compared on their ability to detect the most common pathogens reported to be associated with RCR: Fusarium solani, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Macrophomina phaseolina.Significant differences (P

Next Generation Sequencing (NGS), conventional Polymerase Chain Reaction (PCR), and classical fungal culturing methods were compared in identification of …

Effect Of Dung Beetles On Dung Decomposition And Nutrient Cycling In A Nebraska Rangeland, Kenneth Shay Evans

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Management practice can have impacts on the abundance and frequency of dung beetle populations and nutrient cycling in grazing systems. Also, agriculture and livestock production land use is a considerable source of anthropogenic greenhouse gas (GHG) emissions, which are known to be one of the causes of global climate change. In this study, we evaluated the effect of dung beetle presence on the fluxes of greenhouse gasses (GHG’s) from dung pats in the semi-arid Sandhills region of Nebraska, by using closed chambers to measure the fluxes of carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (N …

The Preservation And Protection Of Native Biodiversity In The Guadalupe Nipomo Dunes Complex, Lindsey M. Whitaker, Matthew Ritter, Scott J. Steinmaus, Jonathan Hall

Master's Theses

The Guadalupe Nipomo Dunes Complex (GNDC) is located within the California Floristic Province, a biodiversity hotspot characterized by high rates of endemism and exceptional loss of habitat. In 1980, the US Fish and Wildlife Service described the GNDC as, “the most unique and fragile ecosystem in the State of California,” and ranked it first on a list of 49 habitat areas needing state protection. It is the largest coastal dune area in California and it is one of the last remaining, relatively intact ecosystems of its type and size in the western United States. The growing recognition of species decline …

Opposing Roles Of Foliar And Glandular Trichome Volatile Components In Cultivated Nightshade Interaction With A Specialist Herbivore, Lucy Kananu Murungi, Hillary Kirwa, Daisy Salifu, Baldwyn Torto

All Peer-Reviewed Publications

Plant chemistry is an important contributor to the interaction with herbivores. Here, we report on a previously unknown role for foliar and glandular trichome volatiles in their interaction with the specialist herbivore of solanaceous plants, the tomato red spider mite Tetranychus evansi.We used various bioassays and chemical analyses including coupled gas chromatography- mass spectrometry (GC/MS) and liquid chromatography coupled to quadrupole time of flight mass spectrometry (LC-QToF-MS) to investigate this interaction between cultivated African nightshades and T. evansi.We show that, whereas morphologically different cultivated African nightshade species released similar foliar volatile organic compounds (VOCs) that attracted T. evansi, VOCs released …

Coevolution Of Cyanogenic Bamboos And Bamboo Lemurs On Madagascar, Daniel J. Ballhorn, Fanny Patrika Rakotoarivelo, Stefanie Kautz

Biology Faculty Publications and Presentations

Feeding strategies of specialist herbivores often originate from the coevolutionary arms race of plant defenses and counter-adaptations of herbivores. The interaction between bamboo lemurs and cyanogenic bamboos on Madagascar represents a unique system to study diffuse coevolutionary processes between mammalian herbivores and plant defenses. Bamboo lemurs have different degrees of dietary specialization while bamboos show different levels of chemical defense. In this study, we found variation in cyanogenic potential (HCNp) and nutritive characteristics among five sympatric bamboo species in the Ranomafana area, southeastern Madagascar. The HCNp ranged from 209±72 μmol cyanide* g^-1 dwt in Cathariostachys madagascariensis to no cyanide …

High Throughput Phenotypic Evaluation Of Drought-Related Traits In Soybean, Hua Bai

Graduate Theses and Dissertations

Drought limits crop growth and yield in soybean. Rapid and effective methods of screening large numbers of soybean lines for drought tolerance are urgently needed. Two experiments were conducted to evaluate the effects of drought in soybean during reproductive stages. In the first experiment five genotypes from maturity groups 2 through 5 were tested under well-irrigated and drought conditions. Beginning at R5, leaf samples were taken for nitrogen concentration analysis. Pictures were taken across the top of each plot to determine the intensity of greenness using the Dark Green Color Index (DGCI). Aerial photographs were also taken to determine aerial …

Assessment Of Concentrations Of Trace And Toxic Heavy Metals In Soil And Vegetables Grown In The Vicinity Of Manyoni Uranium Depositin Tanzania, F.A. Kapile, In Makundi

Tanzania Journal of Science

This study reports on determination of concentrations of trace and toxic heavy metals in soil and vegetables grown near of Manyoni uranium deposit. Soil and vegetable samples were collected from five sites namely Mitoo Mbuga, farming area, Miyomboni, Tambukareli and near water pump. The concentrations of heavy metals in soil and edible vegetables samples were analyzed using Energy Dispersive X-ray Fluorescence Spectrometry (EDXRF).All vegetable samples were found to have higher concentrations (in μg/g) of trace elements such as Ni (67.3) in pea leaves, Cu (14.9) in pumpkin leaves, Fe (478.6), (200.5) and (337.1) in pea, pumpkin and spinach leaves respectively, …

Functional Diversity Of Small And Large Trees Along Secondary Succession In A Tropical Dry Forest, Lucía Sanaphre-Villanueva, Juan Manuel Dupuy, José Luis Andrade, Casandra Reyes-García, Horacio Paz, Paula C. Jackson

Faculty Articles

Functional Diversity is considered an important driver of community assembly in environmental and successional gradients. To understand tree assembly processes in a semideciduous tropical forest, we analyzed the variation of Functional Richness (FRic), Functional Divergence (FDiv), and Functional Evenness (FEve) of small vs. large trees in relation to fallow age after slash-and-burn agriculture and topographical position (flat sites vs. hills). FRic of small trees was lower than null model predicted values across the successional gradient, and decreased unexpectedly in older successional ages. FRic of large trees was higher than null model predictions early in succession and lower in late-successional stands …

Storage Of Pigeonpea Grain (Cajanus Cajan (L.) Millsp.) In Hermetic Triple-Layer Bags Prevents Losses Caused By Callosobruchus Maculatus (F.) (Coleoptera: Bruchidae), H. D. Affognon, A. W. Njoroge, C. M. Mutungi, J. Manono, D. Baributsa, L. L. Murdock

All Peer-Reviewed Publications

Dry pigeonpea grain is susceptible to infestation from the cowpea bruchid during storage. This study investigated the potential of Purdue Improved Cowpea Storage (PICS™) and polypropylene (PP) bags for reducing insect infestation under ambient laboratory conditions for six months. Some of the bags were artificially infested with cowpea bruchid Callosobruchus maculatus (PICS1, PP1) while others were not (PICS0, PP0). In an additional trial, PP bags containing pigeonpeas were treated with Actellic Super® dust before being artificially infested (PP1Ac). Moisture content, number of live C. maculatus adults, grain damage, and weight loss were determined at monthly intervals. Temperature and relative humidity …

Leaf Protein And Mineral Concentrations Across The “Miracle Tree” Genus Moringa, Mark E. Olson, Renuka P. Sankaran, Jed W. Fahey, Michael A. Grusak, David Odee, Wasif Nouman

Publications and Research

The moringa tree Moringa oleifera is a fast-growing, drought-resistant tree cultivated across the lowland dry tropics worldwide for its nutritious leaves. Despite its nutritious reputation, there has been no systematic survey of the variation in leaf nutritional quality across M. oleifera grown worldwide, or of the other species of the genus. To guide informed use of moringa, we surveyed protein, macro-, and micro- nutrients across 67 common garden samples of 12 Moringa taxa, including 23 samples of M. oleifera. Moringa oleifera, M. concanensis, M. stenopetala, an M. concanensis X oleifera hybrid, and M. longituba were highest in protein, with M. …

Assessment Of Potential Risks Of Dietary Rnai To A Soil Micro-Arthropod, Sinella Curviseta Brook (Collembola: Entomobryidae), Huipeng Pan, Linghua Xu, Jeffrey E. Noland, Hu Li, Blair D. Siegfried, Xuguo Zhou

Entomology Faculty Publications

RNAi-based genetically engineered (GE) crops for the management of insect pests are likely to be commercialized by the end of this decade. Without a workable framework for conducting the ecological risk assessment (ERA) and a standardized ERA protocol, however, the utility of RNAi transgenic crops in pest management remains uncertain. The overall goal of this study is to assess the risks of RNAi-based GE crops on a non-target soil micro-arthropod, Sinella curviseta, which could be exposed to plant-protected dsRNAs deposited in crop residues. Based on the preliminary research, we hypothesized that insecticidal dsRNAs targeting at the western corn rootworm, …

Regulation Of Plant Productivity By Carbon-Based Nanomaterials: Approach And Evaluation Of Risks, Mohamed Hassen Lahiani

Theses and Dissertations

Carbon-based nanomaterials (CBN) are a unique type of nanomaterials with promising application in different fields including, medicine, genetic engineering, and plant biology. Nanomaterials-plant interaction is a new and emerging area of Nanobiotechnology. Some nano-sized materials are described as promising candidates for the regulation of plant growth, reduction of plant diseases, and delivery of molecules (herbicides, DNA, proteins) inside plant cells and to specific tissues. This study evaluated the potential use of different CBN to stimulate seed germination and plant growth of wide-range of valuable crops. We demonstrated the positive effect of short-term and long-term CBN exposure on the seed germination, …