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Bumpers College Students In Action, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Political Ecology Of Medicinal Plant Use In Rural Nepal: Globalization, Environmental Degradation, And Cultural Transformation, Emily Dovydaitis

Honors Undergraduate Theses

Prior to the advent of biomedicine, rural communities in Nepal relied on phytochemically active compounds in medicinal plants as their primary source of medicine; however, ethnobotanical practices have shifted over time due to economic, environmental, and sociocultural stimuli. Findings from 2016 fieldwork conducted in Dumrikharka, Nepal and Tutung, Nepal are compared to existing literature to describe the political ecology of medicinal plants in rural Nepal.

Anthropogenic climate change threatens individual plant species and ecosystem biodiversity. Globalized markets unabated by weak conservation programs place increasing demands on medicinal plants. As indigenous plants become overharvested and more difficult to access, Nepalis incorporate …

A Survey Of Non-Crop Plants As Alternative Hosts To Raspberries For Drosophila Suzukii (Spotted Wing Drosophila), Bennur Agbaba

Electronic Theses and Dissertations

The spotted wing fruit fly (SWD), Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), is a pest of small fruit crops. Unlike most other Drosophila species, this insect can oviposit into ripe fruits, rendering them unmarketable. Drosophila suzukii is spreading quickly throughout the continental United States including South Dakota, and causing serious damage to horticultural crops, particularly those within the fruit industry. This study determines the D. suzukii host plant both commercial crops and native plants, and defines non-crop host plants by season in South Dakota. Further, it confirms the occurrence of the fly in southeastern South Dakota and identifies high-risk infestation areas …

Response Of Soil Properties To 23-25 Years Of Diverse Crop Rotations And Tillage Systems In South Dakota, Usa, Abdullah H. Alhameid

Electronic Theses and Dissertations

Diverse cropping systems and no-till (NT) are beneficial in improving soil organic carbon (SOC) and other soils properties. The present study was conducted to evaluate the interactions and impacts of long-term tillage and diverse crop rotation systems on soil quality indicators that include a range of soil chemical, physical, hydrological, and microbial properties. The study was established in 1991 at Beresford, South Dakota and included three crop rotation systems [2-yr; maize (Zea mays L.)-soybean (Glycine max L.), 3-yr; maize-soybean-oat (Avena sativa L.), and 4-yr; maize-soybean-oat-wheat (Triticum aestivum L.)] managed under two tillage systems (NT and CT; conventional- tillage). Soil sampling …

Diaporthe, Soybean Cyst Nematode, And Soybean Aphid: An Evaluation Of Potential Interactions Occurring Among Pests On Soybean In South Dakota, John Philip Posch

Electronic Theses and Dissertations

Soybean Glycine max (L.) Merr., is an important crop grown in South Dakota, with an estimated production of $2.33 billion in 2015. However, soybean production in South Dakota is compromised by four pests, the fungal pathogens Diaporthe longicolla (Hobbs) Santos, Vrandecic, and Phillips and Diaporthe caulivora (Athow and Caldwell) Santos, Vrandecic, and Phillips, the soybean cyst nematode Heterodera glycines Ichinohe (SCN), and the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae). Additionally, these pest can co-occur in fields within South Dakota and the implications associated with interactions among these pests are unknown. We hypothesized that both Diaporthe species would interact with …

Detection And Characterization Of Xanthomonas Vasicola Pv. Vasculorum (Cobb 1894) Comb. Nov. Causing Bacterial Leaf Streak Of Corn In The United States, J. M. Lang, E. Ducharme, J. Ibarra Caballero, E. Luna, T. Hartman, M. Ortiz-Castro, K. A. Korus, J. Rascoe, T. A. Jackson-Ziems, K. Broders, J. E. Leach

Department of Plant Pathology: Faculty Publications

Bacterial leaf streak of corn (Zea mays) recently reached epidemic levels in three corn-growing states and has been detected in another six states in the central United States. Xanthomonas vasicola was identified as the causal agent of this disease. A multilocus sequence alignment of six housekeeping genes and comparison of average nucleotide identity from draft genome sequence were used to confirm phylogenetic relationships and classification of this bacteria relative to other X. vasicola strains. X. vasicola isolates from Nebraska and South Africa were highly virulent on corn and sugarcane and less virulent on sorghum but caused water-soaking symptoms …

Response Of Sorghum Stalk Pathogens To Brown Midrib Plants And Soluble Phenolic Extracts From Near Isogenic Lines, Deanna L. Funnell-Harris, Patrick M. O'Neill, Scott E. Sattler, Tammy Gries, Mark A. Berhow, Jeffrey F. Pedersen

Department of Plant Pathology: Faculty Publications

Sorghum [Sorghum bicolor (L.) Moench] has drawn attention as potential feedstock for lignocellulosic biofuels production, and reducing lignin is one way to increase conversion efficiency. Little research has been previously conducted to assess the response of reduced lignin sorghum lines to the Fusarium stalk rot pathogens Fusarium verticillioides and Fusarium proliferatum and the charcoal rot pathogen, Macrophomina phaseolina. Loss of function mutations in either the Brown midrib (Bmr) 6 or 12 gene that both encode a monolignol biosynthetic enzyme in the pathway that produces subunits of the lignin polymer, results in reduced lignin content. Near-isogenic bmr6, …

Impact Of A High Oil And Protein On Agronomic Traits And Overall Seed Composition In Soybean, Maythem Al-Amery

Theses and Dissertations--Plant and Soil Sciences

New soybean lines have been developed with significantly higher oil, protein + oil and higher meal protein. These soybeans contain a VgD1 gene (highly active acyl-CoA:diacylglycerol acyltransferase, DGAT from Vernonia galamensis (VgDGAT1A) produces much higher oil synthesis and accumulation activity in soybean. Soybean with active DGAT from Vernonia galamensis (VgDGAT1A) has active TAG biosynthesis relative to other DGATs including from soybeans and Arabidopsis. DGATs catalyze the final step of TAG synthesis: DAG (diacylglycerol) + acyl-CoA → TAG + CoASH (Coenzyme A is notable for its role in the synthesis and oxidation of fatty acids, and the oxidation of pyruvate in …

Mitigating Risks Of Ochratoxin A Contamination In Oats, Bandana Dhungana

Electronic Theses and Dissertations

Ochratoxin A (OTA) is a toxic metabolite produced by several species of fungal genera Penicillium and Aspergillus. The major OTA producer in temperate regions (i.e. U.S., Canada, Europe) is Penicillium verrucosum. Oat (Avena sativa), like any other cereal grain, can be contaminated with OTA when storage conditions are favorable for fungal growth. The presence of OTA exceeding the European Union maximum limit of 3 ppb in processed food has been reported in some samples of oat-based breakfast cereals from the US. The use of oat genotypes with limited OTA accumulation would be an effective way to …

Perimeter Cropping As A Means Of Reducing Insect Injury And Increasing Crop Yield, Brett L. Owens

Electronic Theses and Dissertations

The squash vine borer, Melittia cucurbitae Harris, (Lepidoptera: Sesiidae) is a destructive insect known to injure a broad range of cucurbit crops. Many studies have focused on insecticide management of this insect in squash (Cucurbita) and pumpkin (Cucurbita pepo Linnaeus) production; however, little work has been documented on organic approaches to reduce or eliminate injury from this pest to these crops in the Midwest. This study used trap cropping and pollinator mixtures to test whether a reduction of squash vine borer could be achieved in a specific cultivar of pumpkins, Cucurbita pepo ‘Howden’. The study was …

Improvement Of Milling And Nutritional Quality Characteristics Of Oats Through Genomic Selection, Sudha Neupane Adhikari

Electronic Theses and Dissertations

Oats can lower cholesterol, reduce risks of type-2 diabetes, and help prevent heart diseases when consumed daily. Therefore, it is important to evaluate and select breeding lines with desirable milling and nutritional quality traits. Genomic selection, which uses genotyping data to predict the breeding value of an individual, is a promising method to increase genetic gain by selecting for quality traits earlier in the line development process. In this study, we collected phenotypic data for three nutritional traits (protein, β- glucan, and fat content) and five milling quality traits (percent plumps, percent thins, percent plump groat, groat percent and thousand …

Soil Response To Cropping Sequences And Grazing Under Integrated Crop-Livestock System, Hanxiao Feng

Electronic Theses and Dissertations

Integrated crop-livestock system (ICLS) is helpful in diversifying a farm for improving its long-term sustainability and economic benefits. In the United States, the ICLS has been increasing in recent years because of its economic and environmental benefits. However, the impacts of ICLS on soil quality is not well documented in North Dakota. The objective of our study is to assess the impacts of cropping sequences and cattle grazing on the selected soil properties in the crop diversity and livestock integration practice. This study site was established in 2010 at the Dickinson Research Extension Center, Dickinson, North Dakota. The study design …

Effects Of Root Isoflavonoids And Hairy Root Transformation On The Soybean Rhizosphere Bacterial Community Structure, Laura White

Electronic Theses and Dissertations

Rhizodeposits play a key role in shaping rhizosphere microbial communities. In soybean, isoflavonoids are a key rhizodeposit component that aid in plant defense and enable symbiotic associations with rhizobia. However, it is uncertain if and how they influence rhizosphere microbial communities. Isoflavonoid biosynthesis was silenced via RNA interference of isoflavone synthase in soybean hairy root composite plants. Successive sonication was implemented to isolate soil fractions from 3 different rhizosphere zones at 1 and 3 weeks post planting. PCR amplicons from 16S rRNA gene variable regions V1- V3 and V3-V5 from these soil fractions were analyzed via denaturing gradient gel electrophoresis …

Impacts Of Integrated Crop-Livestock System On Soil Surface Greenhouse Gas Fluxes At A Farm Scale In South Dakota, Brant Douville

Electronic Theses and Dissertations

Grazing and cover crops (CC) can impact soil nutrient cycling but may increase the greenhouse gas (GHG) emissions from soils. However, little is known about their impacts on soil properties and soil surface GHG emissions in an integrated crop-livestock system (ICLS) in South Dakota, USA. The present study was conducted at a farm scale at the Research Farm of South Dakota State University, Brookings, South Dakota, to assess the impact of cover crops and grazing on soil pH, electrical conductivity (EC), and bulk density (BD), and soil surface carbon dioxide (CO₂), methane (CH₄), and nitrous oxide …

Interactive Effects Of Cover Crops, Invertebrate Communities And Soil Health In Corn Production Systems, Claire Lacanne

Electronic Theses and Dissertations

The adoption of regenerative farming practices is gaining traction, but the costs and benefits are not often considered on a systems level. Encouraging biodiversity and soil health is the goal of many agricultural practices used in regenerative farming; regenerative systems employ practices which abide by the two main principles of increasing biodiversity and decreasing disturbance, with the goal of encouraging ecosystem functioning to minimize inputs and maximize the productivity of a farm. I examined the management of corn (Zea mays) fields across four states in the Upper Midwest region of the United States. Regenerative systems in this study …

Identification Of Physiological And Morphological Traits Governing High Water Use Efficiency In Alfalfa, Krishna Ghimire

Electronic Theses and Dissertations

Alfalfa is an important forage crop worldwide. Being deep-rooted, N2-fixing and high yielding, alfalfa has great economic, ecological and nutritional benefits. While alfalfa is a high yielding crop, its high productivity depends on irrigation water in many areas and consumes the greatest amount of water among all the major crops. With a growing demand for water resources due to an increase in human population and industrial water use, plus frequent drought due to climate change, irrigation water has become increasingly scarce and expensive. To sustain high production of alfalfa with limited water resource, alfalfa cultivars with improved water use efficiency …

Elucidation Of Abscisic Acid In Stress Resistance Of Mnium Cuspidatum Through Salt Exposure And Chlorophyll Content Analysis, Kurt Corsbie

Undergraduate Honors Thesis Collection

Upon exposure to stresses, a battery of reactions occurs within the model moss Physcomitrella patens that induces the expression of defense-related genes. One of these reactions results in the production of abscisic acid (ABA), a plant hormone that helps a plant resist desiccation by inducing the activation of defense genes which results in protective physiological reactions. In this study, experiments were conducted on the moss species Mnium cuspidatum to see if it responds similarly to P. patens. This comparison was investigated as sample groups of M. cuspidatum and P. patens were pre-treated with 0µM, 50µM, or 500µM ABA to …

Guide To Quaking Aspen Ecology And Management With Emphasis On Bureau Of Land Management Lands In The Western United States, Paul C. Rogers

Aspen Bibliography

No abstract provided.

Aspen Health On National Forests In The Northern Rocky Mountain Region, James T. Blodgett, Kurt K. Allen, Kendra Schotzko, Al Dymerski

Aspen Bibliography

No abstract provided.

Assessment Of Aspen Ecosystem Vulnerability To Climate Change For The Uinta-Wasatch- Cache And Ashley National Forests, Utah, Janine Rice, Tim Bardsley, Pete Gomben, Dustin Bambrough, Stacey Weems, Allen Huber, Linda A. Joyce

Aspen Bibliography

No abstract provided.

Reproductive Biology Of The Critically Endangered Endemic Planterodium Somanum In Turkey, Di̇lek Oskay

Turkish Journal of Botany

The reproductive characteristics of Erodium somanum (Geraniaceae), a critically endangered endemic species, were investigated in order to evaluate the main factors that affect its reproductive success and to evaluate their importance on its conservation. The estimated pollen/ovule ratio was calculated as 1187. Pollen viability and stigma receptivity experiments showed that stigma receptivity and pollen viability were high and synchronous throughout the anthesis. E. somanum is a strictly dioecious species and pollinated by insects. Flower visitors in the studied populations were mostly beetles and ants. The average percentage of fruit formation for each plant was determined as 46%. Capacity of seed …

Dianthus Ucarii (Caryophyllaceae): A New Species From The Northwest Of Turkey, Ergi̇n Hamzaoğlu, Murat Koç, İlker Büyük

Turkish Journal of Botany

Dianthus ucarii Hamzaoğlu & Koç (Caryophyllaceae) is described as a new species to science based on material collected in Kırklareli Province in northwestern Turkey. The new taxon belongs to sect. Carthusiani (Boiss.) F.N.Williams and it is closely related to Dianthus pinifolius Sm., an Eastern Mediterranean element. A description, illustration, and identification key for the new species are given. In addition, D. ucarii and D. pinifolius are compared in terms of general morphology and molecular phylogeny.

An Ethnobotanical Survey In Selected Districts Of The Black Sea Region (Turkey), Emi̇ne Burcu Yeşi̇lyurt, Işil Şi̇mşek, Gali̇p Akaydin, Erdem Yeşi̇lada

Turkish Journal of Botany

The current study was carried out in selected districts of the Black Sea region of Turkey, in Trabzon (center), Çorum (İskilip town), Karabük (Yenice town), Amasya (Kapıkaya village), and Amasya (Belmebük village) in order to document the wild plants used by the local inhabitants, i.e. as a remedy or foodstuff or for other purposes. The data were collected from 352 individuals residing in these districts and volunteering to participate in the research through face-to-face interviewing. Depending on the data collected, the parts of the plants used, the purpose in using them, and the ways of preparation (simply cooking or decoction, …

Studies On The Floral Anatomy And Scent Chemistry Of Titan Arum(Amorphophallus Titanum, Araceae), Vijayasankar Raman, Nurhayat Tabanca, Betül Demi̇rci̇, Ikhlas Khan

Turkish Journal of Botany

Amorphophallus titanum (Becc.) Becc. ex Arcang., the titan arum, is popularly known for having the world's largest unbranched inflorescence. The enormous flower-like inflorescence is also called 'corpse flower' or 'carrion flower' due to the characteristic putrid odor of the bloom. The present study illustrates detailed anatomy and micromorphology of the inflorescence by light and scanning electron microscopy. Collenchyma was found to be the major tissue providing mechanical support in the spathe. The appendix consisted of a dense parenchymatous cortex enclosing a hollow center traversed by loose networks of aerenchyma. Anthocyanin pigments found in the epidermises and cortical tissues imparted a …

Determining Diurnal And Seasonal Changes In Melatonin Andtryptophan Contents Of Eggplant (Solanum Melongena L.), Ahmet Korkmaz, Gökçen Yakupoğlu, Şebnem Köklü, Yakup Cuci̇, Feri̇t Kocaçi̇nar

Turkish Journal of Botany

The objective of this study was to simultaneously determine changes in tryptophan and melatonin contents of eggplant seedlings at two different growth stages during a 24-h period. Variations in tryptophan and melatonin levels in different organs (roots, leaves, fruits, and seeds) and the distribution and accumulation of these molecules in these organs during various growth stages were also investigated. In eggplant seedlings, melatonin showed a strong peak at the beginning of the dark period, while tryptophan levels were at the lowest. Melatonin was also high in the middle of the light period and an inverse relationship was observed between melatonin …

Karyotype Analysis Of Some Taxa Of Dianthus Section Verruculosi (Caryophyllaceae, Sileneae), Derya Altay, Hali̇l Erhan Eroğlu, Ergi̇n Hamzaoğlu, Murat Koç

Turkish Journal of Botany

The purpose of this study was to determine chromosome numbers and karyological features of six Dianthus taxa belonging to section Verruculosi from Anatolia, Turkey. The chromosome numbers were determined as 2n = 30 in all taxa. Karyological measurements were made in detail for the first time for all taxa. Chromosome counts were given for the first time for all taxa except D. cyri and D. strictus. A new chromosome number was determined for D. strictus. The intrachromosomal and interchromosomal karyotype asymmetries were estimated with MCA and CVCL values.

Zn-Use Efficiency For Optimization Of Symbiotic Nitrogenfixation In Chickpea (Cicer Arietinum L.), Igor Kryvoruchko

Turkish Journal of Botany

Zn deficiency is widespread in traditional areas of chickpea cultivation worldwide. It limits chickpea productivity and causes significant losses to the economies of the world?s largest chickpea exporters. This review may be of interest to researchers who would like to contribute to the improvement of chickpea cultivation on Zn-depleted soils in an environmentally sustainable manner, namely via identification of genotypes with superior symbiotic performance under Zn-limited conditions. The primary aim of the current work is to familiarize the readers with the biology and symbiotic characteristics of chickpea, and also to provide the necessary background on Zn as an essential nutrient …

Yuhina Nigrimenta Blyth (Zosteropidae) As A Bird Pollinator Ofbrandisia Hancei Hook.F. (Scrophulariaceae) During Winter, Yifan Qian, Yun Xiang Li, Xuemei Zhang, Qiu Mei Quan

Turkish Journal of Botany

Winter-flowering plants commonly attract an insufficient number of pollinators. Harsh climate conditions, such as low temperature, make it impossible for insects to evolve as reliable pollinators. Birds can buffer against changes in weather to be more reliable pollinators for these kinds of plants, such as Brandisia hancei Hook.f. (Scrophulariaceae), a shrub endemic to the Northeast Sichuan Basin in China. In this study, the flower visitors, flowering phenology, pollinators, breeding system, and nectar availability of B. hancei Hook.f. were investigated. Results show that the flowering period duration was approximately 3 months for the whole population and more than 10 days per …

Structural Studies Demonstrating A Bacteriophage-Like Replication Cycle Of The Eukaryote-Infecting Paramecium Bursaria Chlorella Virus-1, Elad Milrot, Eyal Shimoni, Katya Rechav, Tamar Unger, James L. Van Etten, Abraham Minsky

James Van Etten Publications

A fundamental stage in viral infection is the internalization of viral genomes in host cells. Although extensively studied, the mechanisms and factors responsible for the genome internalization process remain poorly understood. Here we report our observations, derived from diverse imaging methods on genome internalization of the large dsDNA Paramecium bursaria chlorella virus-1 (PBCV-1). Our studies reveal that early infection stages of this eukaryotic- infecting virus occurs by a bacteriophage-like pathway, whereby PBCV-1 generates a hole in the host cell wall and ejects its dsDNA genome in a linear, base-pair-by-base-pair process, through a membrane tunnel generated by the fusion of the …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.