Digital Commons Network^™

Isolation Of Sperm Storage Tubules From The Uterovaginal Junction Mucosa Of The Turkey, L. M. King, J. P. Brillard, M. R. Bakst, A. M. Donoghue

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study was performed to determine whether intact sperm storage tubules (SST) could be successfully isolated from the uterovaginal junction (UVJ) mucosa of the turkey. Large White BUTA hens were inseminated and euthanatized 24 to 48 h later. Oviducts were excised, UVJ tissue removed, and SST were procured by enzymatic digestion. Recovered SST were intact and contained motile sperm. The sperm were oriented with their acrosomes pointed towards the distal end of the SST, and their long axes in parallel with the long axis of the tubule’s lumen. This method for the isolation of intact SST can be readily applied …

Cytogenetic Assignment Of 53 Microsatellites From The Usda-Marc Porcine Genetic Map, N. L. Lopez-Corrales, C. W. Beattie, G. A. Rohrer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study provides 53 new fluorescent in situ hybridization cytogenetic assignments for microsatellite markers linked to the swine genetic map. Forty microsatellites are physically assigned for the first time. The chromosomal locations of eight markers were either confirmed or refined, while five loci were assigned to locations different from those given in previous reports. Markers were selected to provide physical anchors based on their presumed proximity to centromeres or telomeres and at approximately 30 cM invervals across the genetic map. The number of physical anchors for pig (SSC) chromosomes 8, 15, and 18 linkage groups was significantly improved. Centromeric regions …

Effect Of Airflow On House Fly (Diptera: Muscidae) Distribution In Poultry Houses, Christopher Geden, Jerome Hogsette, Roger Jacobs

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Numbers of fecal and vomit spots deposited by house flies, Musca domestica L., on spot cards were about twice as high on cards placed on the downwind sides as on the upwind sides of building support posts in caged-layer poultry houses with tunnel ventilation in Brooksville, FL. This trend was stronger at the ends of the houses where airflow is faster than in the relatively still-air center of the houses. A similar evaluation conducted in a pullet house (Zephyrhills, FL) with an evaporative cooling ventilation system revealed significantly higher fly counts on spot cards and sticky cards in downwind compared …

The Reproductive Biology And Effective Pollinators Of The Endangered Beardtongue Penstemon Penlandii (Scrophulariaceae), Vincent J. Tepedino, Sedonia D. Sipes, Terry L. Griswold

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Penland's beardtongue, a rare endemic plant of the Colorado Plateau, displays a mixed breeding system. Plants are partially self-compatible but set more fruits when cross-pollinated than when self-pollinated. Fruit production is significantly increased by pollinators. However, in two years of study there was no indication that fruit set was being limited by inadequate pollinator visitation. Pollinator effectiveness was judged by correlating bee behavior at the flowers with analysis of the pollen carried on bee bodies. The most important pollinators were native megachilid bees, particularly in the genus Osmia. The bees that pollinate Penland's beardtongue are essential to its reproduction …

Report Of The Brassica Crops Working Group, Chris Neeser

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Brassicaceae family comprises about 3000 species, the majority of which are found in the Northern Hemisphere. Many common agricultural weeds, such as Brassica nigra (L.) Koch, Brassica rapa L., Cardaria draba (L.) Desv., Raphanus raphanistrum L., and Sinapis arvensis L., belong to this family. The most important crop species from this family are the oilseed Brassicas; Brassica napus L., B rapa L., and B juncea Coss., which are generally referred to as rapeseed, oilseed rape, or canola. Other widely cultivated species in this family are: B. oleracea L. (cabbage, kale, kohlrabi, Brussels sprouts, cauliflower, and broccoli), B. chinensis L. …

A Review Of Issues Pertaining To Transgenic Turfgrasses, Paul G. Johnson, Terrance P. Riordan

Department of Agronomy and Horticulture: Faculty Publications

Recombinant DNA technology is a rapidly growing field in plant and animal breeding. This technology involves the transfer of pieces of DNA, or genes, regardless of the organisms involved or how they are related. Transformation procedures in plants were first developed for important crop and model plant systems. Although turfgrass management and production is one of the fastest growing areas of agriculture, genetic transformation of turfgrasses lags behind that of many other important crop plants. Turfgrasses are becoming more important primarily because of their association with the ever-increasing urban population. The size of the turfgrass seed market is second only …

Plant Hybrid Zones And Insect Host Range Expansion, Diana Pilson

Diana Pilson Publications

The hybrid bridge hypothesis suggests that plant hybrids ‘‘bridge’’ the genetic gap between actual and potential host species, and that, for this reason, herbivorous insects are more likely to evolve an expanded host range in the presence of hybrids. While intuitively appealing, the hypothesis has two implicit assumptions: that phenotypic gaps between potential hosts limit host range, and that characters controlling host use are additively inherited in plant hybrids. Evaluation of these assumptions suggests that operation of the hybrid bridge hypothesis is relatively uncommon. In addition, the hypothesis has not been well integrated into existing theoretical and empirical work on …

Report Of The Cucurbit Working Group, James D. Mccreight, Jack Staub

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cucumber (Cucumis sativus L.) originated in India, melon (C. melo L.) and watermelon (Citrullus lanatus) in Africa, and squash, pumpkin, and gourd (Cucurbita spp.) in the Americas. Thus, cucumber, melon, and watermelon (including citron) are relatively recent introductions to the New World. Most domesticated species of Cucurbita were introduced from Mexico, Central America, and South America with the migration of native Americans centuries earlier. Wax gourd (Benincasa hispida (Thunb.) Logr.) is from Southeast Asia. Bottle gourd (Lagenaria siceraria (Molina) Stand.) is of African origin. South Asia is the probable center of …

Physical Mapping Of The Bovine, Caprine And Ovine Homologues Of The Paired Box Gene Pax8, N. L. Lòpez-Corrales, T. S. Sonstegard, T. P. L. Smith

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The PAX8 gene, a member of the human paired box gene family, was mapped by FISH to chromosome 11 in cattle and goat and to the short arm of chromosome 3 in sheep. The cytogenetic position of PAX8 on BTA 11 and on its homologue OAR 3p lies in the region where the interleukin beta (IL1B) gene has been previously located, (BTA 11q22.1→q22.3 and OAR 3p25→q26 respectively; Lòpez-Corrales et al., 1998). The results indicated that PAX8 as well as interleukin beta and interleukin alpha (IL1B and IL1A) genes detected on the human chromosome segment HSA 2q13→q21 maintain a similar …

Analysis Of Human Skin Emanations By Gas Chromatography/Mass Spectrometry. 1. Thermal Desorption Of Attractants For The Yellow Fever Mosquito (Aedes Aegypti) From Handled Glass Beads, Ulrich R. Bernier, Matthew M. Booth, Richard A. Yost

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Handled glass has the ability to collect and concentrate nonaqueous human skin emanations while minimizing the collection of aqueous perspiration. Compounds originating from the skin and collected on glass have previously been found to attract the Aedes aegypti species of mosquito. Therefore, glass beads were used as the medium to collect skin emanations from humans for subsequent chemical analysis. This process consisted of a 5-15-min collection of sample on glass beads, followed by loading the beads into a gas chromatograph (GC) injector insert for subsequent desorption of the collected compounds onto the GC column. After cryofocusing by liquid nitrogen at …

Promotor (Flt) For The Full-Length Transcript Of Peanut Chlorotic Streak Caulimovirus (Pclsv) And Expression Of Chimeric Genes In Plants, Indu B. Maiti, Robert J. Shepherd

Kentucky Tobacco Research and Development Center Faculty Patents

The isolation, modification and use of wild-type and modified viral FLt promoters of peanut chlorotic streak caulimovirus (PClSV) in the expression of chimeric genes in plant cells. The FLt promoter from PClSV has been modified to have duplicated enhancer domains.

Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 1998, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This report of the Central Crops and Soils Research Station at Highmore, South Dakota is a progress report and, therefore, the results presented are not necessarily complete nor conclusive. Any interpretation given is tentative because additional data from continuation of these experiments may produce conclusions different from those of any one year. The data presented in this report reflect the 1998 growing season.

Bulletin No. 36: Amphibians And Reptiles Of The Connecticut College Arboretum, Jill Devito, Joseph Markow

Bulletins

No abstract provided.

Southeast South Dakota Experiment Farm Annual Progress Report, 1998, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This thirty-eighth annual report of the research program at the Southeast South Dakota Experiment Farm has special significance for those engaged in agriculture and the agriculturally related businesses in the nine county area of southeast South Dakota. Reports in this document include information on: temperatures and precipitation data, corn production and performance, soybean research and planting, soil testing, alfalfa yield test, fertilizer testing, herbicide research, crop rotation, sorghum, small grains, livestock research, and pest and weed control.

Nebline, December 1998

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Holiday legends,traditions & fun
Gardening resolutions
Keep trees fresh
Cyclamen care
Painting with plants
Pruning vines and groundcovers
Combing—a non-toxic method that can eliminate head lice
Our Christmas tree
A mouse in the house
A “must read” book
It’s a hit–20,000 hits!
Use and care of home humidifiers
Deadline near for requesting free inspection in compliance with Livestock Waste Management Act
Private applicators training
Crop Protection Clinic
The Power of a PTO
Beef home study course begins fifth year
Landlords need to reduce cash crop rental rates for '99
Developing your marketing plan
Chain saw operation
Commercial handling of fresh …

Genetic Variation In Chrysopsis Floridana Small, The Endangered Florida Golden Aster, As Revealed By Random Amplification For Polymorphism Detection (Rapd), Laurie Lysle Walker Markham

USF Tampa Graduate Theses and Dissertations

Chrysopsis fioridana Small (Asteraceae) is a federally endangered plant endemic to the Tampa Bay area of west central Florida. It is confined to the sand pine scrub community, growing in open, sunny, and sandy areas or in disturbed areas at the edges of scrub. As a means ofassessing the genetic variability ofthis species, eight populations in Hillsborough County were compared with a seed stock collection housed at Bok Tower Gardens in Lake Wales using random amplification for polymorphism detection (RAPD). The objectives of this study were to (I) describe the overall genetic variation among and within populations and subpopulations of …

Fecal Bacteria Survival And Infiltration Through A Shallow Agricultural Soil: Timing And Tillage Effects, C. S. Stoddard, Mark S. Coyne, John H. Grove

Plant and Soil Sciences Faculty Publications

Human and livestock exposure to fecal pathogens via contaminated surface or groundwater is an important water quality concern for soils receiving animal wastes. The effects of manure application timing (spring or fall application) and soil management (no-tillage or conservation tillage) on fecal bacteria infiltration through shallow karst soils in central Kentucky (the Bluegrass region) have not been evaluated. We performed a field experiment to measure fecal coliforms and fecal streptococci in leachate from dairy manure-amended no-tillage and conservation tillage soils. Manure significantly increased fecal bacteria in leachate compared with unmanured treatments. After manure application, the leachate that collected in zero-tension …

Nebline, November 1998

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Auntie Bacteria's holiday advice
Gifts for the gardener
Forcing bulbs
Attracting birds with the right plants
Rodent damage
Winter care for strawberry plants
Dealing with head lice? Even for experts, it's a head scratcher
Good reasons to recycle aluminum
Milk carton bird feeder
Migrating birds face many hazards
Achieving cleaner indoor air
Holding wet corn with aeration
Grain storage information on the web
Pesticide container recycling a success
Farm finances tight? Fertilize with biosolids and save $$
Acreage reply
Native prairie preservation
United States using less water
Reduce orchard woes
Mealtime tips for the preschool child
Healthy Eating: Holiday Turkey …

Forage News [1998-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forage Conference a Success
• KFGC Award Winners
• Congratulations to Dr. Powell
• Landmark Event for Forages in Nebraska
• Hay Bales Don’t Always “Weigh-Up” to Expectations
• Minimize Losses in Hay Storage and Feeding
• Evening Grazing is More Efficient
• Roundup Ready Alfalfa?
• Endophyte in Perennial Ryegrass
• Preference by Ruminants Among Tall Fescue Cultivars
• Upcoming Events

Potential Of The Entomopathogenic Fungus, Metarhizium Anisopliae (Metsch.) Sorokin For Control Of The Legume Flower Thrips, Megalurothrips Sjostedti (Trybom) On Cowpea In Kenya, S. Ekesi, N. K. Maniania, K. Ampong-Nyarko, I. Onu

All Peer-Reviewed Publications

Field experiments were conducted at Mbita, western Kenya for two seasons to evaluate the potential of the entomopathogenic fungus, Metarhizium anisopliae (Metsh.) Sorokin, for biological control of the legume flower thrips, Megalurothrips sjostedti (Trybom), on cowpea. An ultra-low-volume (ULV) oil/aqueous formulation and a high-volume (HV) aqueous formulation of conidia were applied three times each at two concentrations of 1 x 1011 and 1 x 1013 conidia ha-1. Compared with the untreated controls, both formulations significantly reduced thrips populations and plant damage in both seasons. Adult insects collected from fungal-treated plots and reared in the laboratory showed a high mortality due …

Signaling Via Camp In Fungi: Interconnections With Mitogen-Activated Protein Kinase Pathways, James Kronstad, Adriana De Maria, Deanna Funnell, R. David Laidlaw, Nancy Lee, Mário Moniz De Sá, Marilee Ramesh

Department of Plant Pathology: Faculty Publications

The cAMP signal transduction pathway controls a wide variety of processes in fungi. For example, considerable progress has been made in describing the involvement of cAMP pathway components in the control of morphogenesis in Saccharomyces cerevisiae, Ustilago maydis, and Magnaporthe grisea. These morphological processes include the establishment of filamentous growth in S. cerevisiae and U. maydis, and the differentiation of an appressorial infection structure in M. grisea. The discovery that appressorium formation requires cAMP signaling provides an immediate connection to fungal virulence. This connection may have broader implications among fungal pathogens because recent work indicates …

Bird Use Of Riparian Vegetation Along The Truckee River, Califonia And Nevada, Suellen Lynn, Michael L. Morrison, Amy J. Kuenzi, Jennifer C. C. Neale, Benjamin N. Sacks, Robin Hamlin, Linnea S. Hall

Great Basin Naturalist

The Truckee River in California and Nevada is subject to diverse water regimes and a corresponding variety of flow rates. Original riparian vegetation has been altered by these variable flow rates and by a variety of human uses resulting in loss of native riparian vegetation from its historic extent. We conducted bird surveys along the Truckee River during spring 1993 to (1) determine relationships between birds and the present vegetation; (2) determine the importance of different vegetation types to sensitive bird species that have declined recently in the western United States due to competition from exotic plant species, cowbird ( …

The Pseudomonas Syringae Pv. Tomato Hrpw Protein Has Domains Similar To Harpins And Pectate Lyases And Can Elicit The Plant Hypersensitive Response And Bind To Pectate, Amy O. Charkowski, James R. Alfano, Gail Preston, Jing Yuan, Sheng Yang He, Alan Collmer

Center for Plant Science Innovation: Faculty Publications

The host-specific plant pathogen Pseudomonas syringae elicits the hypersensitive response (HR) in nonhost plants and secretes the HrpZ harpin in culture via the Hrp (type III) secretion system. Previous genetic evidence suggested the existence of another harpin gene in the P. syringae genome. hrpW was found in a region adjacent to the hrp cluster in P. syringae pv. tomato DC3000. hrpW encodes a 42.9-kDa protein with domains resembling harpins and pectate lyases (Pels), respectively. HrpW has key properties of harpins. It is heat stable and glycine rich, lacks cysteine, is secreted by the Hrp system, and is able to elicit …

Center For Grassland Studies Newsletter, Fall 1998, Volume 4, No. 4

Center for Grassland Studies: Newsletters

Contents:

Subirrigated Meadow Management Pratices by Jerry Volesky, West Central Research and Extension Center, UNL

Wildlife Habitat Incentive Program by Craig Derickson and Gerald Jasmer, USDA, Natural Resources Conservation Service

The Effect of Planting Date on Turfgrass Seed Production in the High Plains by Rebecca Harms, Turfgrass Project Coordinator, UNL

Beef Home Study Courses Begin Fifth Year

Plan Now to Attend SRM/AFGC Joint Meeting

Forage News [1998-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Fall Grazing School
• One Day Grazing Schools
• Forage Conference
• Forages at KCA
• Prussic Acid Poisoning
• Drought Stressed Pastures
• Upcoming Events

Forage News [1998-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Dollars & Cents of Stockpiling Tall Fescue
• KFGC Award Nominations Due October 9
• Kentucky Grazing School
• KFGC Fall Forage Conference
• Alfalfa Conference March 4
• Forages at the State Fair
• Maximize Grazing - Minimize Stored Feed
• Warm Season Grass Field Day
• Kentucky Pasture & Hay Conditions
• Forages at KCA
• Hay Quality Standards
• Upcoming Events

The Prairie Naturalist Volume 30, No. 3 September 1998

The Prairie Naturalist

GROWTH OF SPOTIED BASS IN KANSAS STREAMS AND IMPOUNDMENTS ▪ J. S. Tillma and C. S. Guy

SIZE SELECTION OF RAINBOW SMELT BY SMALL MESH GILL NETS ▪ C. E. Freiburger and W. G. Duffy

ABUNDANCE AND HABITAT AFFINITIES OF Cyclotracheius substriatus (Coleoptera: Carabidae) ON NORTHERN SHORTGRASS PRAIRIE ▪ N. E. Mcintyre 157

THE REPRODUCTIVE BIOLOGY OF CANDLE ANEMONE ▪ B. Molano-Flores and S. D. Hendrix

NOTES

Noteworthy Records of Bats from Southern Colorado ▪ E. W. Valdez

Eggshell Removal by Duck Hens Following

Partial Nest Depredation by Striped Skunk ▪ S. Lariviere and L. R. Walton

New Records of …

Optimization Of Solid-State Fermentation Parameters For The Production Of Xylanase By Trichoderma Longibrachiatum On Wheat Bran, Elizabeth R. Ridder, Sue E. Nokes, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

Solid-state fermentation has the potential to produce inexpensive enzymes for use in high-volume industrial applications. Process parameters such as substrate moisture content and length of fermentation can have a significant effect on the amount and timing of enzyme production. This study was conducted in two stages, a screening stage and an optimization stage, to determine the effects of moisture content of the substrate, surfactant addition upon inoculation, depth of the substrate, and duration of fermentation on xylanase activity produced by Trichoderma longibrachiatum. Screening fermentations were conducted at 25°C, 50 and 75% wet basis moisture content (w.b.), 0.0 and 0.2% …

Interactions Between The Structural Domains Of The Rna Replication Proteins Of Plant-Infecting Rna Viruses, Erin K. O'Reilly, Zhaohui Wang, Roy C. French, C. Cheng Kao

Department of Plant Pathology: Faculty Publications

Brome mosaic virus (BMV), a positive-strand RNA virus, encodes two replication proteins: the 2a protein, which contains polymerase-like sequences, and the 1a protein, with N-terminal putative capping and C-terminal helicase-like sequences. These two proteins are part of a multisubunit complex which is necessary for viral RNA replication. We have previously shown that the yeast two-hybrid assay consistently duplicated results obtained from in vivo RNA replication assays and biochemical assays of protein-protein interaction, thus permitting the identification of additional interacting domains. We now map an interaction found to take place between two 1a proteins. Using previously characterized 1a mutants, a perfect …

Nebline, September 1998

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

National 4-H Week: October 4-10
Saving geraniums over winter
Perennials forever
Drying gourds
Seasonal needle drop on evergreens
Shades of autumn
“Why are we finding all these little reddish-brown beetles in our new home?”
Celebrate National Pollution Prevention (P2) Week
Birds of wonder: chimney swifts
Managing deer populations and damage
Bumblebees pollinating more
Recycling facts
Making the “right” management choices
Hard to control pasture weeds—fall is the time for control
Handle hay bales safely
Are you looking for the latest wheat variety test results?
Farm first-aid kits need to be user-friendly
Yes, no, or what?
Are you considering non-traditional livestock …