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Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Manure Matters, Volume 9, Number 9, Christopher G. Henry, Mike Reynolds

Manure Matters (newsletter)

Low Water Stream Crossings for Cattle (LWSC)

Livestock access to streams, specifically, cattle access to streams in pastures has become of concern in the development of Total Maximum Daily Loads (TMDLs). There is very little information available to planners on the contribution of nitrogen, ammonia, phosphorus, and human health-related microorganisms to stream segments from rangeland cattle. A common modeling solution in the development of TMDLs is to exclude livestock from streams in order to reduce nutrient loads. Although this approach seems simplistic, it is problematic for owners and managers of rangeland livestock.
Many producers are now choosing to supply water …

Manure Matters, Volume 9, Number 8, Laura Hillenbrand

Manure Matters (newsletter)

Excerpt from Seabiscuit

The halcyon days at the Tijuana track came to a spectacular end. On the backstretch early each morning men guided teams of horses on circuits of the barns, shoveling the mucked-out manure into wagons and driving the teams up the hill behind the backstretch, where they would dump it. The pile had been accumulating since 1917, and because the city received little rain to wash it down, it was enormous. “Oh my gosh.” Remembered trainer Jimmy Jones. “ It was as big as the grandstand.” Inside its depths, the manure fermented, generating scalding heat.
To the locals, …

Manure Matters, Volume 9, Number 7, Christopher G. Henry

Manure Matters (newsletter)

Land Application Setback and Buffer Requirements for NPDES Permitted Large CAFOs

Large Concentrated Animal Feeding Operation (CAFO) owners/operators are required to implement setbacks, buffers, or an alternative conservation practice on all fields to which manure is applied. This newsletter discusses the federal rules and some guidance on how the rule may be applied to land application sites. The setbacks and buffer requirements apply to large CAFOs only. Most states are authorized to implement the CAFO program and may have additional, more stringent requirements. Check with your state permitting authority to determine the requirements that apply to your operation.

Manure Matters, Volume 9, Number 6, Ron Sheffield, Juli Paschold

Manure Matters (newsletter)

The New CAFO Rules: What is Required in a Nutrient Management Plan?

As a part of the U.S. Environmental Protection Agency’s (EPA) Concentrated Animal Feeding Operation (CAFO) Final Rule, all CAFO owners are required to apply for a National Pollutant Discharge Elimination System (NPDES) permit. Part of the process includes developing and implementing a Nutrient Management Plan (NMP). At a minimum, an NMP must include Best Management Practices (BMPs) and, for large CAFOs, procedures necessary to achieve effluent limitation guidelines (ELGs).

Manure Matters, Volume 9, Number 4, Richard K. Koelsch

Manure Matters (newsletter)

New EPA Rules Targeting Livestock and Poultry Industry Congress passed the Clean Water Act (CWA) in 1972 to "restore and maintain the chemical, physical, and biological integrity of the Nation's waters." Among its core provisions, it prohibits the discharge of pollutants from a point source to waters of the United States except as authorized by an NPDES permit. EPA's regulation of concentrated animal feeding operations (CAFOs) under the CWA dates to the 1970s. EPA established effluent guidelines for feedlots in 1974 based on the best available technology that was economically achievable for the industry. CAFO regulations issued in 1976 determined …

Manure Matters, Volume 9, Number 2, Richard K. Koelsch, Wendy Powers

Manure Matters (newsletter)

Integrating Animal Feeding Decisions into CNMP Processes: Part 2

This is part 2 of a two part series, part one was discussed in Volume 9, Number 1.

Manure Matters, Volume 9, Number 1, Richard K. Koelsch, Wendy Powers

Manure Matters (newsletter)

Integrating Animal Feeding Decisions into CNMP Processes: Part 1

Environmental planning in animal production systems often requires an estimate of nutrient excretion. Standard values published by the Natural Resources Conservation Service (NRCS) (SCS 1992), American Society of Agricultural Engineers (ASAE 1999), and MidWest Plan Service (MWPS 2000) commonly have been used for this purpose. However, these current procedures do not reflect the impact of producers' animal dietary decisions on nutrient excretion. The increasing variety of feed ingredient options, changes in nutritional programs to match improving genetic potential, and feeding strategies designed to reduce nutrient excretion are influencing the amount of …

Nebraska Vine Lines, Volume Vi, Number 3, June/July 2003

Nebraska Vine Lines (2003–2006)

FIRST NEBRASKA WINE FESTIVAL A REAL “HAPPENING”

James A. Johnson

A FEW SUMMER REMINDERS

APPLE TWIG BORER UP-DATE

Grape Cane Borer

Grape Cane Borer Biology and Control

FIRST ANNUAL FOUR-STATE FIELD DAY FOR 2003

TELEPHONE NUMBERS NEEDED

The Nebraska State Fair, the NWGGA and the Nebraska Wine and Grape Industry

The NWGGA Board’s Message

From the NWGGA Membership Committee:

Winery updates

2003 Nebraska Entrepreneur Company of the Year

A Successful First Year!

TO OUR NEW FRIENDS:

SWING INTO SUMMER

LOVERS LEAP VINEYARD

Gold has struck us again! A Gold medal at the Tasters Guild for our 2002 Edelweiss and a …

Avoiding The “Fat” Of The Land: Case Studies Of Agricultural Nutrient Balance, David Weaver, Simon Neville

Journal articles

Let’s start with a simple analogy: if a person eats more than they need, they gain weight. That is: if our feed inputs (kilojoules in) are greater than our outputs (exercise — kilojoules out) then we will gain weight (kilojoules in storage).That’s our fat. If, on the other hand, our feed inputs are less than our outputs, then we will lose weight.And if our inputs are the same as our outputs, our weight will remain constant. In general, the further away you are from an ‘ideal’weight, the greater the health risks. And yes, other aspects of your body management — …

Olfactometric Evaluation Of Spatial Repellents For Aedes Aegypti, Daniel L. Kline, Ulrich R. Bernier, Kenneth H. Posey, Donald R. Barnard

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The spatial repellency responses of Aedes aegypti (L.) to deet, dehydrolinalool and linalool were evaluated using a dual port olfactometer. In the absence of human attractant mixture, each of the three chemicals resulted in activation and/or orientation of mosquitoes to the chemical source. Linalool was the most attractive compound. In the presence of human attractant mixture, activation and/or orientation of mosquitoes to each of the three chemicals was reduced. We compared reductions in mosquito responses to each of the three chemicals, in the presence of human attractant mixture, to estimate spatial repellency. As expected, lowest spatial repellency (7.3%) was observed …

Description And Analysis Of Two Internet-Based Databases Of Insect Pathogens: Edwip And Vidil, S. M. Braxton, D. W. Onstad, D. E. Docktor, R. Giordano, R. Larsson, R. A. Humber

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In 1996, two searchable databases covering insect pathogens were posted on the World Wide Web: the Ecological Database of the World's Insect Pathogens (EDWIP) and the Viral Diseases of Insects in the Literature database (VIDIL). In this paper, we describe the format and contents of EDWIP and VIDIL on the World Wide Web. EDWIP contains over 9400 pathogen–host association records, 677 negative test result or ‘‘no association’’ records, 4454 host species, 2285 pathogen species records, and 2057 bibliographical references. Species of Coleoptera and Lepidoptera are the best represented groups in EDWIP. Lepidopteran species account for the most associations of any …

Forage Yield And Economic Losses Associated With The Brown-Midrib Trait In Sudangrass, M. D. Casler, Jeffrey F. Pedersen, D. J. Undersander

Department of Agronomy and Horticulture: Faculty Publications

Brown-midrib genes increase digestibility due to reduced lignification in sudangrass, Sorghum bicolor subsp. drummondii (Nees ex Steud.) de Wet & Harlan. Brown-midrib lines are known to be low in forage yield potential, but this reduction in forage yield has not been previously quantified. The objectives of this study were to quantify the increase in forage quality and decrease in forage yield and to provide an economic assessment of this dichotomy. Piper and Greenleaf (normal leaves) were compared with their brown-midrib counterparts and four highly selected brown-midrib (FG) lines at two locations for 2 yr. Brown-midrib lines averaged 9.0% lower in …

Are Chlorella Viruses A Rich Source Of Ion Channel Genes?, Ming Kang, Anna Moroni, Sabrina Gazzarrini, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Plaque-forming dsDNA (>330 kb) viruses that infect certain unicellular, eukaryotic chlorella-like green algae contain ~375 protein-encoding genes. These proteins include a 94 amino acid K+ channel protein, called Kcv, as well as two putative ligand-gated ion channels. The viruses also encode other proteins that could be involved in the assembly and/ or function of ion channels, including protein kinases and a phosphatase, polyamine biosynthetic enzymes and histamine decarboxylase.

Fungal Biology And Agriculture: Revisiting The Field, O. Yarden, D. J. Ebbole, S. Freeman, R. J. Rodriguez, M. B. Dickman

Department of Plant Pathology: Faculty Publications

Plant pathology has made significant progress over the years, a process that involved overcoming a variety of conceptual and technological hurdles. Descriptive mycology and the advent of chemical plant-disease management have been followed by biochemical and physiological studies of fungi and their hosts. The later establishment of biochemical genetics along with the introduction of DNA-mediated transformation have set the stage for dissection of gene function and advances in our understanding of fungal cell biology and plant–fungus interactions. Currently, with the advent of high-throughput technologies, we have the capacity to acquire vast data sets that have direct relevance to the numerous …

The Black Dump Fly: A Larval Predator Of House Flies, J.A. Hogsette, R.D. Jacobs

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The black dump fly ( Figure 1 ) is a shiny, black fly similar in size and appearance to the house fly. It is currently regaining popularity as a biological control agent for controlling house flies on poultry farms without the use of pesticides. The fly is native to the U.S., and Central and South America. The current scientific name of the fly is Hydrotaea aenescens, however it is sometimes referred to by its former name, Ophyra aenescens. The purpose of this document is to provide some general information about this fly, its biology and behavior, and how …

Growth Of Forage Legumes And Grasses In Acidic Soil Amended With Flue Gas Desulfurization Products, R. B. Clark, V. C. Baligar

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Large amounts of flue gas desulfurization products (FGDs) are produced when SO₂ emissions are trapped in the coal burning process for generation of electricity. FGDs are normally discarded instead of being reused, and reuse on soils could be important in overall management of these products. Glasshouse experiments were conducted to determine effects of various levels of three FGDs (a FGD gypsum, an oxidized FGD + Mg, and a stabilized FGD) and the control compounds CaCO₃, CaSO₃, and CaSO₄ on growth of alfalfa (Medicago sativa), white clover (Trifolium repens), orchardgrass ( …

Potential Relationships Between Physical Traits And Male Broiler Breeder Fertility, S. Mcgary, I. Estevez, M. R. Bakst

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Genetic selection in primary broiler breeders may modify skeletal structure, possibly impeding semen transfer, and could alter the size and degree of fluctuating asymmetry (FA) of bilateral traits associated with fertility. Hence, we hypothesized specific morphometric traits could predict differential fertility. Sixty primary broiler breeder males from Strains A and B (n = 30/strain) were individually housed with an average of 10 females per male. Male fertility and sperm penetration (SP) through the perivitelline layer were estimated on fresh eggs. At 50 wk, BW, keel length (KL), posterior pelvic width and length (PPW, PPL), dorsal pelvic width and length (DPW, …

To Be Or Not To Be A Locust? A Comparative Analysis Of Behavioral Phase Change In Nymphs Of Schistocerca Americana And S. Gregaria, Gregory A. Sword

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Phenotypic plasticity in behavior induced by high rearing density is often part of a migratory syndrome in insects called phase polyphenism. Among locust species, swarming and the expression of phase polyphenism are highly correlated. The american grasshopper, Schistocerca americana, rarely swarms even though it is closely related to the swarming Old World desert locust, S. gregaria, as well as two swarming New World locusts. Anecdotal field observations of locust-like behavior in S. americana indicate that it may express behavioral phase polyphenism, but empirical investigations are lacking. In this study, I tested the hypothesis that S. americana expresses locust-like …

Nebline, January 2003

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Family Leadership Key to Community Leadership
Gardening Resolutions
Garden Guide
Humidity and Plants in the Home Environment
Horticulture Information Center
What Do Birds Like to Eat?
Dealing with Roosting Birds
Chicken Feathers Can Now be Used in Air Purifying Filters
Creepy-Crawlies in your Cereal
Put Farm Leases in Writing for Legal Protection
Private Pesticide Applicator Training Jan. 25, 29 & Feb 10
Check and Aerate Stored Grain
Computerized Financial Record Keeping Workshop March 18
Add Lime Now for Next Spring’s Alfalfa
Protecting Water from Freezing
Fire Safety in the Home
Latest U.S. Drought Monitor Map
Sizing Up Food Portion Sizes …

2003 Survey Of Priority Natural Communities In Jefferson County, Nebraska, Robert F. Steinauer

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

This survey was undertaken to update information on native plant communities and wildlife habitats in Nebraska to facilitate the development of the Nebraska Game and Parks Commission’s (NGPC) Comprehensive Wildlife Plan. The specific intent of the project was to identify and map all large blocks of privately owned native plant communities within the survey area. In 2003, the surveys focused on eastern Nebraska, specifically deciduous forest communities along the Missouri River, tallgrass prairies of southeastern Nebraska (Pawnee, Gage, Jefferson, and eastern Thayer counties), and loess mixed-grass prairies in central Nebraska. A brief survey of the Elkhorn River valley in Madison …

Letter From The Dean, Gregory J. Weidemann

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Single-Nucleotide Polymorphisms In Soybean, Y. L. Zhu, Q. J. Song, D. L. Hyten, C. P. Van Tassel, L. K. Matukumalli, D. R. Grimm, S. M. Hyatt, E. W. Fickus, N. D. Young, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Single-nucleotide polymorphisms (SNPs) provide an abundant source of DNA polymorphisms in a number of eukaryotic species. Information on the frequency, nature, and distribution of SNPs in plant genomes is limited. Thus, our objectives were (1) to determine SNP frequency in coding and noncoding soybean (Glycine max L. Merr.) DNA sequence amplified from genomic DNA using PCR primers designed to complete genes, cDNAs, and random genomic sequence; (2) to characterize haplotype variation in these sequences; and (3) to provide initial estimates of linkage disequilibrium (LD) in soybean. Approximately 28.7 kbp of coding sequence, 37.9 kbp of noncoding perigenic DNA, and …

Impact Of Egg Storage On Carbonic Anhydrase Activity During Early Embryogenesis In The Turkey, M. R. Bakst, L. Holm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Carbonic anhydrase is an enzyme that plays important roles in the conversion of carbon dioxide to bicarbonate, acid-base balance, and in subembryonic fluid formation in the early Japanese quail embryo. While turkey egg storage longer than 10 d is known to increase the rate of embryo mortality, little is known of the biological mechanisms that contribute to this phenomenon. In this study, we examined the impact of turkey egg storage on carbonic anhydrase activity in the freshly laid egg through 72 h of incubation. Carbonic anhydrase activity, which was not affected by egg storage for 21 d at 18°C, was …

Detection Of Enterohemorrhagic Escherichia Coli O157:H7 By Using A Multiplex Real-Time Pcr Assay For Genes Encoding Intimin And Shiga Toxins, Vijay K. Sharma, Evelyn A. Dean-Nystrom

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A multiplex real-time PCR (R-PCR) assay was designed and evaluated on the ABI 7700 sequence detection system (TaqMan) to detect enterohemorrhagic Escherichia coli (EHEC) O157:H7 in pure cultures, feces, and tissues. Three sets of primers and fluorogenic probes were used for amplification and real-time detection of a 106-bp region of the eae gene encoding EHEC O157:H7-specific intimin, and 150-bp and 200-bp segments of genes stx1 and stx2 encoding Shiga toxins 1 and 2, respectively. Analysis of 67 bacterial strains demonstrated that the R-PCR assay successfully distinguished EHEC O157:H7 serotype from non-O157 serotypes and provided accurate profiling of genes encoding intimin …

Enhanced Bioremediation Of Soil Containing 2,4-Dinitrotoluene By A Genetically Modified Sinorhizobium Meliloti, Sisir K. Dutta, Gail P. Hollowell, Fawzy M. Hashem, L. David Kuykendall

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The symbiotic nitrogen-fixing soil bacterium, Sinorhizobium meliloti, is well known for its ability to interact with the leguminous plant Medicago sativa L. It has, however, not been reported that this species possesses the capability to degrade toxic nitroaromatic compounds, such as 2,4-dinitrotoluene (DNT) which is commonly associated with the degradation of the explosive trinitrotoluene (TNT). In this study, the pJS1 DNT-biodegradative plasmid was genetically transferred to S. meliloti strain USDA 1936, which was confirmed by plasmid profile analysis. Several standard analytical and chemical tests including high performance liquid chromatography (HPLC), nitrite (NO2) release assays, rhizosphere population and plant greenhouse …

Upland Rice And Allelopathy, N. K. Fageria, V. C. Baligar

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Upland rice (Oryza sativa L.) is mainly grown in Asia, Africa and Latin America. Yield potential of upland rice is quite low and invariably this crop is subjected to many environmental stresses. Further, when upland rice is grown in monoculture for more than two to three years on the same land, allelopathy or autotoxicity is frequently reported. Allelopathy involves complex plant and plant chemical interactions. The level of phytotoxicity of allelochemicals is influenced by abiotic and biotic soil factors. Adopting suitable management strategies in crop rotation can reduce or eliminate allelochemicals phytotoxicity. Rice yields can be improved by growing …

Leaching Of Phosphorus Fractions Following Manure Or Compost Application, Bahman Eghball

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Phosphorus (P) leaching in manure-amended soil can be influenced by the P fractions added when manure or composted manure is applied. This study was conducted to determine leaching of different P fractions following beef cattle feedlot manure or compost application. Manure and composted manure were applied to meet the nitrogen (N) or P needs of corn (Zea mays L.) for either a one or two-yr period. Fertilized plots and unfertilized control plots were also used. The P-based treatments also received additional N fertilizer. Soil P fractions were determined for various soil depth increments. After 4 years of manure and …

Evaluation Of Quinoa (Chenopodium Quinoa Willd) At Different Spacings Between Furrows And Plants In The Mamani Community, José Ferrufino Miranda

Theses and Dissertations

This study addressed spacing between furrows and plants in the cultivation of the quinoa variety Patacamaya. This study took place from 1999 to 2000 in the Mamani community, Ingavi, La Paz, Bolivia. The Patacamaya variety is a cross between Samaranti (sweet) and Kaslala (bitter), with a vegetative cycle of 145 days (semi-early). It has large grains, is sweet, and has a high grain yield. The spaces between furrows were 40, 60, and 80 cm in an experimental design of randomly divided plots with four repetitions. The spaces between furrows were implemented in large plots and the plant spaces as sub-treatments …