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The Plant Structure Ontology, A Unified Vocabulary Of Anatomy And Morphology Of A Flowering Plant, Katica Ilic, Elizabeth Kellogg, Pankaj Jaiswal, Felipe Zapata, Peter Stevens, Leszek Vincent, Shulamit Avraham, Leonore Reiser, Anuradha Pujar, Martin Sachs, Noah Whitman, Susan Mccouch, Mary Schaeffer, Doreen Ware, Lincoln Stein, Seung Rhee Dec 2006

The Plant Structure Ontology, A Unified Vocabulary Of Anatomy And Morphology Of A Flowering Plant, Katica Ilic, Elizabeth Kellogg, Pankaj Jaiswal, Felipe Zapata, Peter Stevens, Leszek Vincent, Shulamit Avraham, Leonore Reiser, Anuradha Pujar, Martin Sachs, Noah Whitman, Susan Mccouch, Mary Schaeffer, Doreen Ware, Lincoln Stein, Seung Rhee

Biology Department Faculty Works

Formal description of plant phenotypes and standardized annotation of gene expression and protein localization data require uniform terminology that accurately describes plant anatomy and morphology. This facilitates cross species comparative studies and quantitative comparison of phenotypes and expression patterns. A major drawback is variable terminology that is used to describe plant anatomy and morphology in publications and genomic databases for different species. The same terms are sometimes applied to different plant structures in different taxonomic groups. Conversely, similar structures are named by their species-specific terms. To address this problem, we created the Plant Structure Ontology (PSO), the first generic ontological …


Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 2006, Agricultural Experiment Station, Plant Science Department Dec 2006

Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 2006, Agricultural Experiment Station, Plant Science Department

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2006 progress report for the Central Crops and Soils Research Station at Highmore, South Dakota State College. This document represents the research conducted at the Station during the 2006 crop season including: temperature and precipitation data, evaluation of native and naturalized grasses for reduced-input turf in the Northern Plains, 2006 NTEP tall fescue ancillary trial for drought tolerance, field evaluations of woody plant materials, alfalfa production, optimal management of drought -tolerant legumes and warm season annual grasses, winter wheat breeding, evaluation of sunflower germplasm for resistance to the red sunflower seed weevil, weed control, fertilizer influence on …


Intsormil’S Global Impact: A Revolution In West African Sorghum Production, Intsormil Dec 2006

Intsormil’S Global Impact: A Revolution In West African Sorghum Production, Intsormil

INTSORMIL Impacts and Bulletins

Sorghum and pearl millet are the staple grains for millions of people around the world, especially those residing in the semiarid margins of agricultural sustainability. For this reason, INTSORMIL has been working in the semiarid regions of Africa and Central America where it has been helping sorghum and millet farmers achieve the status of surplus grain production and the economic, social and health benefits thus provided. What impact has INTSORMIL had on the lives of these sorghum and millet farmers?

To determine the global impact of the USAID funded INTSORMIL program, Battelle, an independent agency, was chosen to conduct an …


Microarray Analysis Of Late-Season Velvetleaf (Abutilon Theophrasti) Effect On Corn, David P. Horvath, Robert Gulden, Sharon A. Clay Dec 2006

Microarray Analysis Of Late-Season Velvetleaf (Abutilon Theophrasti) Effect On Corn, David P. Horvath, Robert Gulden, Sharon A. Clay

Agronomy, Horticulture and Plant Science Faculty Publications

Microarray analysis was used to identify changes in gene expression in corn leaves collected from plants at the V11–14 growth stage that resulted from competition with velvetleaf. The plants were grown in field plots under adequate N (addition of 220 kg N ha1) and irrigation to minimize N and water stress. Consequently, only differences resulting from competition for micronutrients, light, and perhaps allelopathic stress were anticipated. Genes involved in carbon and nitrogen utilization, photosynthesis, growth and development, oxidative stress, signal transduction, responses to auxin and ethylene, and zinc transport were repressed in corn growing in competition with velvetleaf. …


Forage News [2006-12], Department Of Plant And Soil Sciences, University Of Kentucky Dec 2006

Forage News [2006-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

  • Grazing Conference
  • FEED: Biggest Cost Item
  • Heart of America Grazing Conference
  • KFGC Presents Annual Awards
  • KFGC Installs New Officers
  • Fayette County Producer Becomes Forage Spokesperson
  • Kentucky Hay – 2006
  • Methods to Manage Soil Phosphorus Levels on Kentucky Poultry Farms
  • Meat Goat Performance and Carcass Parameters When Finished on Orchardgrass, Red Clover, or Alfalfa Pastures
  • The Case for Forage Legumes
  • Upcoming Events


Evaluating Diet Composition Of Pronghorn In Wind Cave National Park, South Dakota, Christopher N. Jacques, Jaret D. Sievers, Jonathan A. Jenks, Chad L. Sexton, Daniel E. Roddy Dec 2006

Evaluating Diet Composition Of Pronghorn In Wind Cave National Park, South Dakota, Christopher N. Jacques, Jaret D. Sievers, Jonathan A. Jenks, Chad L. Sexton, Daniel E. Roddy

The Prairie Naturalist

The pronghorn (Antilocapra americana) was reintroduced into Wind Cave National Park (WCNP), South Dakota, in 1914, and thus, has inhabited the Park for nearly a century. During the 1990's, a decline in the population raised concern for the continued existence of pronghorn inside WCNP; an investigation into the observed decline was initiated. Primary objectives of our study were to evaluate diet composition and forage selection by pronghorn in WCNP. Microhistological analysis was conducted on 58 fecal samples collected opportunistically from pronghorn during 2002. Blue grama (Bouteloua gracilis), common juniper (Juniperus communis), and northern bedstraw …


Southeast South Dakota Experiment Farm Annual Progress Report, 2006, Agricultural Experiment Station Dec 2006

Southeast South Dakota Experiment Farm Annual Progress Report, 2006, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This document highlights 24 crop and livestock research and demonstration reports from projects conducted at Southeast Research Farm in 2006. It is published by the South Dakota Agricultural Experiment Station and Cooperative Extension Service at South Dakota State University in cooperation with the Southeast South Dakota Experiment Farm Corporation. Reports in this document include information on: temperatures and precipitation data, corn production and performance, soybean research and planting, soil testing, alfalfa yield test, fertilizer testing, herbicide research, crop rotation, sorghum, small grains, livestock research, and pest and weed control.


Perennial Pastures For Western Australia, Geoff Allan Moore, Paul Sanford, Tim Wiley Dec 2006

Perennial Pastures For Western Australia, Geoff Allan Moore, Paul Sanford, Tim Wiley

Bulletins 4000 -

The aim of this project was to identify potential new perennial legumes and grasses to complement lucerne in phase farming and to identify the role and potential for perennial grasses in Western Australia.


Feeding And Managing Sheep In Dry Times, Ian Mcfarland, Mandy Curnow, Mike Hyder, Brian Ashton, Danny Roberts Dec 2006

Feeding And Managing Sheep In Dry Times, Ian Mcfarland, Mandy Curnow, Mike Hyder, Brian Ashton, Danny Roberts

Bulletins 4000 -

Feeding sheep is a significant cost to any sheep or mixed farm enterprise in southern Australia. The cost is usually managed by annually sourcing feed on-farm. However, this feed source can become scarce when we experience unusual dry spells within seasons (termed a 'dry season', such as a dry winter or spring), a late break to the season, a drought year, or even worse, successive drought years.

Climate change research suggests that southern Australia will experience higher annual temperatures and a decline in mean growing season rainfall (particularly winter and spring) over the coming decades. This will affect the productivity …


Bovine Immune Response To Shiga-Toxigenic Escherichia Coli O157:H7, Mark A. Hoffman, Christian Menge, Thomas A. Casey, William Laegreid, Brad T. Bosworth, Evelyn A. Dean-Nystrom Dec 2006

Bovine Immune Response To Shiga-Toxigenic Escherichia Coli O157:H7, Mark A. Hoffman, Christian Menge, Thomas A. Casey, William Laegreid, Brad T. Bosworth, Evelyn A. Dean-Nystrom

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Although cattle develop humoral immune responses to Shiga-toxigenic (Stx+) Escherichia coli O157:H7, infections often result in long-term shedding of these human pathogenic bacteria. The objective of this study was to compare humoral and cellular immune responses to Stx+ and Stx- E. coli O157:H7. Three groups of calves were inoculated intrarumenally, twice in a 3-week interval, with different strains of E. coli: a Stx2- producing E. coli O157:H7 strain (Stx2+O157), a Shiga toxin-negative E. coli O157:H7 strain (Stx-O157), or a nonpathogenic E. coli strain (control). Fecal shedding of Stx2+O157 was …


Amphibians And Reptiles In A Mixed-Grass Prairie In Northwestern North Dakota, Robert K. Murphy, Robert F. Danley, Patricia K. Moore Dec 2006

Amphibians And Reptiles In A Mixed-Grass Prairie In Northwestern North Dakota, Robert K. Murphy, Robert F. Danley, Patricia K. Moore

The Prairie Naturalist

There have been almost no surveys of herpetofauna at 109 km2 Lostwood National Wildlife Refuge (LNWR) or surrounding counties in northwestern North Dakota, an area possibly undergoing significant environmental change from fossil fuel extraction and use. We used 30 m drift fences to survey amphibian and reptile species in prairie-wetland transition zones at LNWR during mid-May to early-July in 1985 to 1987, and again in 1999 and 2000. We captured only four amphibian and two reptilian species and noted one other reptilian species incidental to our survey. Several species expected to occur in the area were not detected.


Kfgc Forage Spokesman Contest, Todd A. Clark, Farms, Inc., Robey Farms Nov 2006

Kfgc Forage Spokesman Contest, Todd A. Clark, Farms, Inc., Robey Farms

Kentucky Grazing Conference

No abstract provided.


New Options For Extending Grazing, Edward N. Ballard Nov 2006

New Options For Extending Grazing, Edward N. Ballard

Kentucky Grazing Conference

Feed costs represent the major cost in most livestock production systems. A recently completed analysis of 225 Standardized Performance Analysis (SPA) Beef Cow Records on herds in Illinois and Iowa showed that feed cost was the overriding factor determining profitability, explaining over 57 percent of the herd-to-herd variation.


Dollars & Cents Of Intensive Grazing, Kenneth H. Burdine Nov 2006

Dollars & Cents Of Intensive Grazing, Kenneth H. Burdine

Kentucky Grazing Conference

A rotational grazing system can generally be defined as the use of several pastures with one being grazed while the others are rested. On the contrary, conventional or continuous grazing systems allow livestock access to the entire pasture area and let them decide where, what, and how long to graze. In most cases, Kentucky pastures employing continuous grazing systems are too large for efficient management and forage utilization. In such instances, cattle overgraze more palatable forages and areas close to shade and water, while other sections of the pasture are underutilized. This leads to lower animal output per acre than …


Uk Horse Pasture Evaluation Program, Tom Keene, S. Ray Smith Nov 2006

Uk Horse Pasture Evaluation Program, Tom Keene, S. Ray Smith

Kentucky Grazing Conference

The University of Kentucky is developing stronger ties with the state’s horse industry in the areas of research, extension and teaching. Many horse farms in the bluegrass region are interested in UK’s assistance with pasture evaluation. During the fall of 2005 a team from the UK Forage Extension Program (Ray Smith, Tom Keene, Jesse Morrison, Gabriel Roberts) conducted a pilot project to evaluate horse pastures on 14 central KY farms. One of the focuses of the pilot was the evaluation of pastures for percent tall fescue and the potential of fescue stands to cause fescue toxicity in pregnant broodmares. We …


Managing Pasture Growth And Quality With Grazing, S. Ray Smith Nov 2006

Managing Pasture Growth And Quality With Grazing, S. Ray Smith

Kentucky Grazing Conference

One of the keys to profitable livestock production is to minimize the costs of producing a marketable animal or animal product. Feed costs are commonly 70-80 percent of the cost of growing or maintaining an animal. Pastures provide feed at a cost of .01-.02 cents/lb of TDN while hay costs .04-.06 cents/lb TDN. Improved pasture management offers the single greatest opportunity to lower production costs, assuming that animal genetics, health, marketing procedures, and other areas of management have been addressed. A primary goal of livestock producers should be to utilize grazed forage for as many months of the year as …


Everyday Cattle Graze Is Money Saved, Garry D. Lacefield Nov 2006

Everyday Cattle Graze Is Money Saved, Garry D. Lacefield

Kentucky Grazing Conference

During one of my first visits to New Zealand over twenty years ago, I had the opportunity to visit many different dairy, beef, sheep and deer farms. While visiting a dairy farm, I remember the farmer telling me about his grazing program and their goal to “optimize grazing and minimize stored feed.” I remember him saying, “everyday grazed is money saved”. I admit, the reality of that statement didn’t truly sink in that day; but over the years, the truth of what he said has not only “sunk in” but has been reinforced by visits to other farms literally around …


Nucleic Acids Encoding Pseudomonas Hop Proteins And Use Thereof, Alan Collmer, James R. Alfano, Xiaoyan Tang, C. Robin Buell, Gregory B. Martin Nov 2006

Nucleic Acids Encoding Pseudomonas Hop Proteins And Use Thereof, Alan Collmer, James R. Alfano, Xiaoyan Tang, C. Robin Buell, Gregory B. Martin

Department of Plant Pathology: Faculty Publications

The present invention relates to isolated nucleic acid molecules encoding a type III—secreted bacterial protein capable of modifying a cell death pathway in a plant cell. One aspect of the present invention involves an isolated nucleic acid molecule having a nucleotide sequence that encodes the HopPtoD2 protein of Pseudomonas syringae pv. syringae DC 3000. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. The nucleic acid mol ecules of the present invention can be used to impart disease resistance to a plant and to make a plant hypersusceptible to colonization …


Forage News [2006-11], Department Of Plant And Soil Sciences, University Of Kentucky Nov 2006

Forage News [2006-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

  • Kentucky Grazing Conference in Lexington, November 21
  • Cash Receipts Highlights – 2005
  • Loss of a Legend
  • Hay Is in the Barn…
  • New Executive Secretary for AFGC
  • Alfalfa’s Makeover
  • Effect of Sericea Lespedeza Hay on Gastrointestinal Nematode Infection in Goats
  • Upcoming Events


Nebline, November/December 2006 Nov 2006

Nebline, November/December 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Extension Offers Guardianship Trainings Mandated By the Nebraska Supreme Court
Guardianships Happen for Variety of Reasons
Pruning Ornamental Plants
Spark Up the Fireplace with Color
Test Nitrogen Levels this Fall, Begin Planning for Spring Fertilization
Free Soybean Cyst Nematode Sample Kits Available
Farming No-Till Increases for Major Nebraska Crops, Better for Environment, Fuel Saved
Computerized Farm Financial Recordkeeping Workshops
Preparing a Thanksgiving Feast Does Not Have to be Difficult!
Monte Cristo Sandwich Recipe
$tretch Your Food Dollar with Healthy Snacks
Time-Saving Kitchen Tools for Holiday Gifts
President’s Notes — Alice’s Analysis
Household Hints: Tips for a Warm, Cozy and Efficient …


Hydrologic Properties Of Pervious Concrete, Joe D. Luck, Stephen R. Workman, Stephen F. Higgins, Mark S. Coyne Nov 2006

Hydrologic Properties Of Pervious Concrete, Joe D. Luck, Stephen R. Workman, Stephen F. Higgins, Mark S. Coyne

Plant and Soil Sciences Faculty Publications

Pervious concrete is concrete made by eliminating most or all of the fine aggregate (sand) in the concrete mix, which allows interconnected void spaces to be formed in the hardened product. These interconnected void spaces allow the concrete to transmit water at relatively high rates. The main objective of this project was to conduct research on the potential application of pervious concrete in agricultural settings, specifically for use in animal feed lots, manure storage pads, animal manure and bedding compost facilities, or floor systems in animal buildings. Laboratory tests were conducted on replicated samples of pervious concrete formed from two …


Development Of Pcr-Based Chloroplast Dna Markers That Characterize Domesticated Cowpea (Vigna Unguiculata Ssp. Unguiculata Var. Unguiculata) And Highlight Its Crop-Weed Complex, Y. Feleke, R. S. Pasquet, P. Gepts Nov 2006

Development Of Pcr-Based Chloroplast Dna Markers That Characterize Domesticated Cowpea (Vigna Unguiculata Ssp. Unguiculata Var. Unguiculata) And Highlight Its Crop-Weed Complex, Y. Feleke, R. S. Pasquet, P. Gepts

All Peer-Reviewed Publications

In 1992, Vaillancourt and Weeden discovered a very important mutation for studying cowpea evolution and domestication. A loss of a BamHI restriction site in chloroplast DNA characterized all domesticated accessions and a few wild (Vigna unguiculata ssp. unguiculata var. spontanea) accessions. In order to screen a larger number of accessions, primers were designed to check this mutation using PCR RFLP or direct PCR methods. Using these new primers, 54 domesticated cowpea accessions and 130 accessions from the wild progenitor were screened. The absence of haplotype 0 was confirmed within domesticated accessions, including primitive landraces from cultivar-groups Biflora and Textilis, suggesting …


Grazing Management Effects On Sediment And Phosphorus In Surface Runoff, Matthew M. Haan, James R. Russell, Wendy J. Powers, John L. Kovar, Jamie L. Benning Nov 2006

Grazing Management Effects On Sediment And Phosphorus In Surface Runoff, Matthew M. Haan, James R. Russell, Wendy J. Powers, John L. Kovar, Jamie L. Benning

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Sediment and phosphorus (P) in runoff from pastures are potential non–point-source pollutants in surface waters that may be influenced by surface cover, sward height, treading damage, surface slope, soil moisture, and soil P. The objectives of the current study were to quantify sediment and total P loads in runoff produced during simulated rainfall from pastures and to evaluate their relationships with the physical and chemical characteristics of the soil and sward. Five forage management treatments— ungrazed (U), hay harvest/fall stockpile grazing (HS), continuous stocking to a sward height of 5 cm (5C), and rotational stocking to sward heights of 5 …


Convergence Of Agriculture And Energy: Implications For Research And Policy, Kenneth Cassman, Vernon Eidman, Eugene Simpson, Larry Berger, Robert Loomis, Marie Walsh, Todd A. Peterson, Edward Runge Nov 2006

Convergence Of Agriculture And Energy: Implications For Research And Policy, Kenneth Cassman, Vernon Eidman, Eugene Simpson, Larry Berger, Robert Loomis, Marie Walsh, Todd A. Peterson, Edward Runge

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Access to an adequate energy supply at reasonable cost is crucial for sustained economic growth. Unfortunately, oil prices and the need to import from politically unstable countries lowers the reliability of the U.S. energy supply and hinders economic development. Although biofuels have been identified as an important component of the national strategy to decrease U.S. dependence on imported oil, the ability to sustain a rapid expansion of biofuel production capacity raises new research and policy issues. This document seeks to identify the most critical of these issues to help inform the policy development process. The goal is to enhance the …


Plant Reproductive Allocation Predicts Herbivore Dynamics Across Spatial And Temporal Scales, Tom E. X. Miller, Andrew J. Tyre, Svata M. Louda Nov 2006

Plant Reproductive Allocation Predicts Herbivore Dynamics Across Spatial And Temporal Scales, Tom E. X. Miller, Andrew J. Tyre, Svata M. Louda

School of Biological Sciences: Faculty Publications

Life-history theory suggests that iteroparous plants should be flexible in their allocation of resources toward growth and reproduction. Such plasticity could have consequences for herbivores that prefer or specialize on vegetative versus reproductive structures. To test this prediction, we studied the response of the cactus bug (Narnia pallidicornis) to meristem allocation by tree cholla cactus (Opuntia imbricata). We evaluated the explanatory power of demographic models that incorporated variation in cactus relative reproductive effort (RRE; the proportion of meristems allocated toward reproduction). Field data provided strong support for a single model that defined herbivore fecundity as a …


Heat Stress Risk Factors Of Feedlot Heifers, Tami M. Brown-Brandl, Roger A. Eigenberg, John A. Nienaber Oct 2006

Heat Stress Risk Factors Of Feedlot Heifers, Tami M. Brown-Brandl, Roger A. Eigenberg, John A. Nienaber

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Heat stress in cattle results in millions of dollars in lost revenue each year due to production losses, and in extreme cases, death. Death losses are more likely to result from animals vulnerable to heat stress. A study was conducted to determine risk factors for heat stress in feedlot heifers. Over two consecutive summers, a total of 256 feedlot heifers (32/ breed/ year) of four breeds were observed. As a measure of stress, respiration rates and panting scores were taken twice daily (morning and afternoon) on a random sample of 10 heifers/ breed. Weights, condition scores, and temperament scores were …


Comparison Of Heat Tolerance Of Feedlot Heifers Of Different Breeds, T. M. Brown-Brandl, J. A. Nienaber, Roger A. Eigenberg, Terry L. Mader, J. L. Morrow, J. W. Dailey Oct 2006

Comparison Of Heat Tolerance Of Feedlot Heifers Of Different Breeds, T. M. Brown-Brandl, J. A. Nienaber, Roger A. Eigenberg, Terry L. Mader, J. L. Morrow, J. W. Dailey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Heat stress in cattle causes decreases in feed intake and feed efficiency; in extreme cases, it can cause death. These losses amount to millions of dollars each year. A study was designed to determine severity of heat stress among four breeds of cattle. Throughout two summers, 256 feedlot heifers of four different breeds were observed. Respiration rates, panting scores, and surface temperatures were taken twice each day on 10 animals/breed for several weeks during the summers of 2002 and 2003. Twenty-four-hour behavior measurements were recorded for four heat-stress and four thermoneutral days. Results showed during the afternoon, Angus cattle (black) …


Heat Stress Risk Factors Of Feedlot Heifers, T. M. Brown-Brandl, Roger A. Eigenberg, J. A. Nienaber Oct 2006

Heat Stress Risk Factors Of Feedlot Heifers, T. M. Brown-Brandl, Roger A. Eigenberg, J. A. Nienaber

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Heat stress in cattle results in millions of dollars in lost revenue each year due to production losses, and in extreme cases, death. Death losses are more likely to result from animals vulnerable to heat stress. A study was conducted to determine risk factors for heat stress in feedlot heifers. Over two consecutive summers, a total of 256 feedlot heifers (32/ breed/ year) of four breeds were observed. As a measure of stress, respiration rates and panting scores were taken twice daily (morning and afternoon) on a random sample of 10 heifers/ breed. Weights, condition scores, and temperament scores were …


Microbial Content Of Abattoir Wastewater And Its Contaminated Soil In Lagos, Nigeria, A. O. Adesemoye, B. O. Opere, S. C. O. Makinde Oct 2006

Microbial Content Of Abattoir Wastewater And Its Contaminated Soil In Lagos, Nigeria, A. O. Adesemoye, B. O. Opere, S. C. O. Makinde

Department of Plant Pathology: Faculty Publications

Microbial content of wastewater in two abattoirs and the impact on microbial population of receiving soil was studied in Agege and Ojo Local Government Areas in Lagos State, Nigeria. Wastewater samples were collected from each of the abattoirs over three months period and examined for microbial content. Soil samples contaminated with the wastewaters were also collected and analyzed for microbial content as compared to soil without wastewater contamination in the neighborhood (control). Some physico-chemical parameters of the samples such as total dissolved solid, chemical oxygen demand, etc., were examined. The wastewater samples from both abattoirs were highly contaminated; Agege abattoir …


Genetic Characterization And Diversity Of Rathayibacter Toxicus, Irina V. Agarkova, A. K. Vidaver, E. N. Postnikova, I. T. Riley, N. W. Schaad Oct 2006

Genetic Characterization And Diversity Of Rathayibacter Toxicus, Irina V. Agarkova, A. K. Vidaver, E. N. Postnikova, I. T. Riley, N. W. Schaad

Department of Plant Pathology: Faculty Publications

Rathayibacter toxicus is a nematode-vectored gram-positive bacterium responsible for a gumming disease of grasses and production of a highly potent animal and human toxin that is often fatal to livestock and has a history of occurring in unexpected circumstances. DNA of 22 strains of R. toxicus from Australia were characterized using amplified fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE). AFLP analysis grouped the 22 strains into three genetic clusters that correspond to their geographic origin. The mean similarity between the three clusters was 85 to 86%. PFGE analysis generated three different banding patterns that enabled typing the strains …